LOCUS       NZ_CP043410             4072 bp    DNA     circular CON 13-JUN-2023
DEFINITION  Escherichia coli strain NMBU-W13E19 plasmid pNMBU-W13E19_04,
            complete sequence.
ACCESSION   NZ_CP043410
VERSION     NZ_CP043410.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12640810
            Assembly: GCF_013282335.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Finton,M.D., Meisal,R., Porcellato,D., Brandal,L.T. and
            Lindstedt,B.-A.
  TITLE     Multidrug-resistant environmental bacterial isolates, Norway
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Finton,M.D., Meisal,R., Porcellato,D., Brandal,L.T. and
            Lindstedt,B.-A.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-AUG-2019) Faculty of Chemistry, Biotechnology and Food
            Science, Norwegian University of Life Sciences, Chr. Magnus
            Falsensv. 1, Aas N-1430, Norway
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 27-AUG-2019
            Assembly Method                   :: Unicycler v. 0.4.7.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 98.0x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/13/2023 03:47:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,247
            CDSs (total)                      :: 5,126
            Genes (coding)                    :: 4,919
            CDSs (with protein)               :: 4,919
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 56 of 207
            Pseudo Genes (incomplete)         :: 158 of 207
            Pseudo Genes (internal stop)      :: 36 of 207
            Pseudo Genes (multiple problems)  :: 41 of 207
            Pseudo Genes (short protein)      :: 4 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP043410.1.
            Bacteria and source DNA available from Prof. B-A. Lindstedt, KBM,
            Norwegian University of Life Sciences, P.O.Box 5003, N-1432 Aas,
            Norway.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="NMBU-W13E19"
                     /serotype="O25:H18"
                     /isolation_source="surface water"
                     /db_xref="taxon:562"
                     /plasmid="pNMBU-W13E19_04"
                     /geo_loc_name="Norway:Aas"
                     /lat_lon="59.667 N 10.769 E"
                     /collection_date="2019-02"
                     /collected_by="Oda Ytreoy"
                     /genotype="ST69"
     gene            complement(join(3550..4072,1..1637))
                     /gene="mobQ"
                     /locus_tag="FZN32_RS25910"
                     /old_locus_tag="FZN32_26645"
     CDS             complement(join(3550..4072,1..1637))
                     /gene="mobQ"
                     /locus_tag="FZN32_RS25910"
                     /old_locus_tag="FZN32_26645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029353.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAECRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     gene            1816..2070
                     /gene="mobC"
                     /locus_tag="FZN32_RS25915"
                     /old_locus_tag="FZN32_26650"
     CDS             1816..2070
                     /gene="mobC"
                     /locus_tag="FZN32_RS25915"
                     /old_locus_tag="FZN32_26650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091747.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     gene            2602..3294
                     /locus_tag="FZN32_RS25920"
                     /old_locus_tag="FZN32_26660"
     CDS             2602..3294
                     /locus_tag="FZN32_RS25920"
                     /old_locus_tag="FZN32_26660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281403.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCISRLKSGADAELQKT
                     MTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKNDEKREEFRWIQYRAQYAKG
                     EAKARITFSDAVMPYLTQLKGQFTRVVIKNISGLSSSYSIRIYEFLQQFRSTGERIIAL
                     NDFRSMLGIENKYKQFRDLNKILIKPCVDELNKKSDLAVTVETIKKGRTVVALHFRFKE
                     DKQIKMTI"
                     /name="replication initiation protein"
     Typing          complement(1280..1638)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.056"
                     /coverage="100.0"
                     /evalue="3.94e-177"
     Typing          1639..1815
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          2603..3294
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.965"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4..1724)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.303"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gactccggcc atgtcttcgg ctggcggaga ttgcgggtat attgcggcca tgtctggcaa
       61 acctgcgtca gaagttgctc aaaggtcgca gaggaataaa cacgttgcag tggtggatgt
      121 tgaccgattt cgcttcggtc acagatataa ccactcctgt cacgcattaa atcaacacgc
      181 tcaaagattc tggtgcggta tttctccagc tcgttccccc agagcgtgta ttctccgttc
      241 cggttgtgaa gcagaaggta acaggtcatt tccccgttac caggcatggg ggcctcgcca
      301 taccccgtaa cgactgcgct cagtacccct gaatccataa cggcattgtc atttttcctg
      361 agctgttccg ctcgtcgctc cgcgcgacta cgttctgtat cctcctgctc cttccggcgc
      421 aattcctctt cacgtttaag caactggact ctgagagctg caaggccgga tgccccccac
      481 tgctgccact gtttttcaaa tgccgtctga ctcgcgggaa tgtcctgttt ctccaccttc
      541 cggtaatgct gtcgcaattt ttccatttgc tggctcagcg actggatttt tccctgttcg
      601 ctacggtatt ccggtgccgc ccattgcatc aggggaatac gggaaaaacc ggacgaaaac
      661 ggcgatgatt ttttcaggct ggcagcatgg ctcatcgctt cctttcgttc ggtgttcagc
      721 tcttcccagc gtctgcgcgt cgcctgcata tctgccatca tcgacatggc attactggtg
      781 agtttccatg ccgcattact gtccttcggc aacgcttcga tgatgtcacg tagtgtgtac
      841 gaagttgcgt tgtcaggaag cacgatattc agaaggtcag ccacacggcg ggcaggctct
      901 gtcagcccgc tgttcctgag ttcagtgcgg gaaatctgta tttccaggtt aagcctgtta
      961 atcagacgat tctgttcgcc gcgttctgtt tcgataccgc gtgtttccat cgcacaggca
     1021 gactttccga ggtgaatacc aggcgttttc tcaagcccct gtcgctcgta tgaacgatgg
     1081 tctatctctt cctgataacc ggcgttcgcc agtgctctgt ttgcatggtc agcccatgat
     1141 gcacgccacg tctcggcatg tgtccggtcg ttccagtccc tgacctttcc gccgaaccca
     1201 gcaggtccga cagccctcgt ggtcagcatg atgtgcgcat ggggatttgt tttatccatg
     1261 tgatggaacg ccacatcagc aatcatgccc tgactgacaa agttttctct gacaaaggcg
     1321 agaacggttt cccgtgcggc atcctgcggc agttcacggg ggatagcgag ttcaatttcc
     1381 cgcgcaagtt gtgagttctt ccggcattcg gccttctcaa cggcattcca gagttcggca
     1441 cggttcgtac accaggcagg ggctgtcgcg ggggtcagaa tcacagcttc agccacaccg
     1501 cttttgcggg tgtagtcgtg agtcagtccg gtacgctcat caagcatcag tgaaccggaa
     1561 cgataggcag cggaagctac ggcgctgtag ccgctgcttc tggagataat tttcatgctg
     1621 agatgataaa tcgccatttc tgccgtaatg cctttttgta gttttctttc agccccgcag
     1681 ggcgcacaca atcgaaacgg agtggagatt gtataagtgc gcactttgtg atttgtcgcg
     1741 cattatgcca gctcagtttt taaactacca gtgacgcgct aaaatgcgat ttttcatcgc
     1801 gcacggagtt acatcatgag cagaacgctc gaacagaaga ttgctgatgc tgaggcgaga
     1861 ttacagcgcc tgaaggcgaa gagccggagc ctggacacag cgcagaaggt tgttgtgggt
     1921 gcagcattgc tggcaaaggt cagaaagccg gaggaggtgc agttgcgtgc ctggttactc
     1981 cagttcctga aggcagaggt gacacgacag gctgatgtga cgcgcatact gccgctgatt
     2041 aacgagctgg aggcgctgcc aggacagtga ttgtatcgtc cgctcgccgc agccccagcg
     2101 accgagcgtt agcgagcgag tgggcgagga agcggaagtg aatccgtcgc caggttgagc
     2161 acatacgcac ggtttttcgg taggtgatat gcgctgaacc cgcatgaata ctggatttcc
     2221 acagggttgg aaggggagta ttcggaactt gcaattctgc tttcttgcaa cacattgttt
     2281 ttattggttt tttgttttca gcgccccaag ttatccacag gctcgccgtt aagtttttag
     2341 atcttgtttt taagatcttg tttttaatac ttgttttagg gtcaaaattc attaataaaa
     2401 tcaatatgtt atagaggtat atgtggagga atttaaggta aaggtggagg aatttaaggt
     2461 gtttaggtgg aggaatttaa ggtaaaggtg gaggaattta aggtgttatt cacagaaatg
     2521 tgaggatata aaaaaaaata ttccacattt cttatttgtg gggcacatta aagcatcacc
     2581 acatttgaac gggatctttg tatgtcggac ttgattgcct acaagtcgaa tgctttggtt
     2641 gaggccagct acaagttgac cctacaggaa caacgttttc tgttgctgtg tatcagtcga
     2701 ttgaaatctg gtgctgatgc tgaattgcaa aagacaatga ccattaccgc tgcggaatat
     2761 tttgattcat tccctgacat ggggcggaaa aatgccgagg ttcagttaca ggaagcgatc
     2821 gatcgtttat gggacaggtc gatcattctc aagaatgacg agaagcgtga ggagttccgc
     2881 tggatccagt atcgggcaca gtacgctaaa ggtgaggcca aggctcgaat aaccttttct
     2941 gacgcggtaa tgccttatct gacgcaactg aaggggcaat tcacgcgcgt tgttataaaa
     3001 aacatatcag gtttatcaag tagttacagt ataagaatat acgagttcct ccagcaattc
     3061 cgcagtacgg gagagagaat tattgctctt aatgactttc gttcaatgtt agggattgaa
     3121 aataaataca aacaattcag agacttaaat aaaatactaa taaaaccctg tgttgacgaa
     3181 ctgaacaaaa aaagcgacct ggctgtaacg gttgagacga tcaaaaaggg gcgcacggtt
     3241 gtagccctgc attttcgctt caaggaagac aaacagatca agatgacgat ctaaaatcga
     3301 ttttaaggca cctaaaacgc tccctgctgc gttttttttg tgttagtagt gcgaaggtac
     3361 tattgaagtg aaaaattgcg tcaaaagcgc tctaatgcag cgaaaaaaga acccccaatt
     3421 tttgggggcg gaaaaacaaa actatgcttg cattatgtgt gtgcttcttg cattcatccc
     3481 tgtcgatgaa catagaagaa catcgacgaa cataacacga tgttaaacaa aacaaaatat
     3541 tgttcacatt cactgtgacc aaccgcgatc atgtctcctc tcggtggtgt gtgttcttgc
     3601 atcttcctcg cgccgccatt catcccaggt acgattgaaa cccagagagc ggaacagatg
     3661 atccggctgt ttttctggtg tgttgacggc agcaaaaagc ggtgcctcac gggttttcag
     3721 atgaaactcc ataagttccg gcgcaggtga tatgtggctg tctgcatcgt gatttacggc
     3781 aacaccatac cctgcatacc gtaacccctt atcggtcagt gcactgatga ccagctggct
     3841 gttatcggct gagggaacgg caaacgcggg atacagcgta ccattaagcc ggacaacacc
     3901 gcccagagag tcatccacgc ccatgaccag caaaagctga agctggcggg tttcccggtc
     3961 gacagaactc attaccggca tcagcgtcgg tagcagacgt ccctgcgtaa aatccacctt
     4021 ccttgctgcc agtgatggca tggctggctg gcggtcttct ccaagacaga aa
//
