LOCUS       NZ_CP043360            10060 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain WCHKP020120 plasmid
            p2_020120, complete sequence.
ACCESSION   NZ_CP043360
VERSION     NZ_CP043360.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07609123
            Assembly: GCF_002852785.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Liu,L., Hu,Y., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Klebsiella pneumoniae strain WCHKP020120
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Liu,L., Hu,Y., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-AUG-2019) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
REFERENCE   3  (bases 1 to 10060)
  AUTHORS   Liu,L., Lu,Y., Lin,J., Yang,B., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2017) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 01:32:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,858
            CDSs (total)                      :: 5,738
            Genes (coding)                    :: 5,589
            CDSs (with protein)               :: 5,589
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 57 of 149
            Pseudo Genes (incomplete)         :: 97 of 149
            Pseudo Genes (internal stop)      :: 17 of 149
            Pseudo Genes (multiple problems)  :: 22 of 149
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP043360.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WCHKP020120"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="p2_020120"
                     /geo_loc_name="China: Sichuan, Chengdu"
                     /collection_date="2017-01-05"
     gene            join(8350..10060,1..254)
                     /locus_tag="CLQ78_RS28985"
                     /old_locus_tag="CLQ78_28965"
     CDS             join(8350..10060,1..254)
                     /locus_tag="CLQ78_RS28985"
                     /old_locus_tag="CLQ78_28965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            254..985
                     /gene="mobC"
                     /locus_tag="CLQ78_RS28990"
                     /old_locus_tag="CLQ78_28970"
     CDS             254..985
                     /gene="mobC"
                     /locus_tag="CLQ78_RS28990"
                     /old_locus_tag="CLQ78_28970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            992..1522
                     /locus_tag="CLQ78_RS28995"
                     /old_locus_tag="CLQ78_28975"
     CDS             992..1522
                     /locus_tag="CLQ78_RS28995"
                     /old_locus_tag="CLQ78_28975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(1549..1728)
                     /locus_tag="CLQ78_RS29000"
                     /old_locus_tag="CLQ78_28980"
     CDS             complement(1549..1728)
                     /locus_tag="CLQ78_RS29000"
                     /old_locus_tag="CLQ78_28980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1754..2182)
                     /locus_tag="CLQ78_RS29005"
                     /old_locus_tag="CLQ78_28985"
     CDS             complement(1754..2182)
                     /locus_tag="CLQ78_RS29005"
                     /old_locus_tag="CLQ78_28985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(2346..2660)
                     /locus_tag="CLQ78_RS29010"
                     /old_locus_tag="CLQ78_28990"
     CDS             complement(2346..2660)
                     /locus_tag="CLQ78_RS29010"
                     /old_locus_tag="CLQ78_28990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2648..2926)
                     /locus_tag="CLQ78_RS29015"
                     /old_locus_tag="CLQ78_28995"
     CDS             complement(2648..2926)
                     /locus_tag="CLQ78_RS29015"
                     /old_locus_tag="CLQ78_28995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(3101..3466)
                     /locus_tag="CLQ78_RS29370"
     CDS             complement(3101..3466)
                     /locus_tag="CLQ78_RS29370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(3463..3834)
                     /gene="tolA"
                     /locus_tag="CLQ78_RS29025"
                     /old_locus_tag="CLQ78_29005"
     CDS             complement(3463..3834)
                     /gene="tolA"
                     /locus_tag="CLQ78_RS29025"
                     /old_locus_tag="CLQ78_29005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(4108..4353)
                     /locus_tag="CLQ78_RS29030"
                     /old_locus_tag="CLQ78_29010"
     CDS             complement(4108..4353)
                     /locus_tag="CLQ78_RS29030"
                     /old_locus_tag="CLQ78_29010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            4680..6365
                     /locus_tag="CLQ78_RS29035"
                     /old_locus_tag="CLQ78_29015"
     CDS             4680..6365
                     /locus_tag="CLQ78_RS29035"
                     /old_locus_tag="CLQ78_29015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            6375..6632
                     /locus_tag="CLQ78_RS29040"
                     /old_locus_tag="CLQ78_29020"
     CDS             6375..6632
                     /locus_tag="CLQ78_RS29040"
                     /old_locus_tag="CLQ78_29020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            6717..6866
                     /locus_tag="CLQ78_RS29045"
                     /old_locus_tag="CLQ78_29025"
     CDS             6717..6866
                     /locus_tag="CLQ78_RS29045"
                     /old_locus_tag="CLQ78_29025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             1..10060
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(6970..7548)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          255..982
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 acagcttcat ccagaaggag gcgcggccgc tgtttgacgt gaacaccctg caacacctgc
       61 ctgacgggtt cgcggtgatg accgggctgg gcgtggcgcg gctggcgttc agcagcccgc
      121 tgcgggttga gcgccgggaa atttcgctga aaaccttccc ggtgctggcg aaaacggacc
      181 cgctggcgga gtacctgcct ccggccgacg gcggcggacc aaaagcggat gatgattttg
      241 cggggcttta ctgatgacac tggagcagta caacgtcagc cacgcgaagc ggcaggcgcg
      301 gaacgcggag aaaacacggc tgaccctgcg ctggctgcgg gaagagctgt gttccacggc
      361 ggagctggtt gcgcgacggc tggggattgc ggcggtacag ccggtttacc gcttcctgga
      421 cagtctggtg gcgaaagggc tgctggtccg ggcgaaatac ccggttgacg gccgtcaggt
      481 cagcgtgtgg gggctgacgc cgcacggggt ggcgttctcg ttcgacgagg acgagccgct
      541 gacggacgtt attccgtttc agccgtcaag ggtgtcggcg gcgcagctgc cgcaccggct
      601 ggccgtgcag tccctgcgcc tctccatgga gggccggggg gccaccggct ggcggtacct
      661 gcaccgtatg gccctgaaag ggatgaaagt gccggacgcg ctggcggagc tggacggcag
      721 aacggtggca tttgaggtag agcgcaccgt caagagccgc aggcggtatc aggaagtggt
      781 gtccgggtat ctgttcaacc gccgggcgaa cggcatcgat gaaatctggt acatctgccc
      841 tgaccggccg acgcttgtcc gggtacagcg ggcgattctg gcggtggatg aaatcgtgaa
      901 tccacagacc ggcgaggccc gcaaaacggt ggagctggac cgggagaggc tgttcgcgtg
      961 ctttaaattt atgacaacgg agtgagggat tatggcgctg tacatggtat ttattgagga
     1021 cgtggaagac aaagacggcg agcgctgcat tatgccaacc agcgagcatc tggaagacat
     1081 ggtggtgctc gcctggcaga ccggggcgta tgttttctgt tttgtcgacc gggcccagca
     1141 tctggcgatg atgaaatcca tcgggatgta tgacgagcgg ctcaacagcg acgaaaaagc
     1201 cacgtacttt ttcaccggtg aaaatccgca atgggaacgg gacaaaccca caggaatgaa
     1261 gctgtgtgat tacctctatc gaaaggtgga gcggttcgcc atcgagaacg gtgtgcaggt
     1321 ggttgatgcc gggaagtgct caccggagca gattaacgcg atgattgagg tggtcagcga
     1381 gcggcacggg gaaagcgcgg tgaacgagaa gctgaaagcg gagctgatgc gggaatataa
     1441 cagccgctat tttaacgtgc cggtgcctga actgccggag gacaaacccg cccgccggtg
     1501 gtggccgttc ggcaaaaaat aacgccagca ccggcatggc cggtgctgtt attcttcccc
     1561 gagcttatcc cgcatcacct gccagagtgt ttccgcatcc tcgtgaagcc gttcgcacat
     1621 gtcggccggg gtatccatgt ccagctcgtt cagcttttcc agcaacagca gcgactgatc
     1681 cttaatgaat ttagccatat tgagggcagt tttttcctgc ttcgtcatgt tctccattcc
     1741 cttcggtaag ttctcagcta aaccagccgg accagaagcg ttttttcttc tgtgtcagtt
     1801 ctgttgtcaa tctgtccact tcggcctgga gtttccggcg ctcatcatcg tgctggcggc
     1861 gttcttcttc ccgggatgtt ttatcttcca gcatcagggt gacagcctgc cggagatcgg
     1921 ccagttcctt ttgcattgcc agaagctgtt cctgtgggaa atttttttgt gtattttcac
     1981 ggtgtgaaat gccgggaatt tgtttctcag ggatattcac atttccgtat acacggatca
     2041 gttctgacac atccacgaca gggttgtttt tcccgtcacg tgacacggta acctttccct
     2101 gttttacatg attgtatagc gttcttctgg taatgcccgc ggcttgtgct gcctgtgaaa
     2161 ggttgagcaa tgttttcgcc actgtatacc cctgatttta tcgggtgtga aaggtgtgta
     2221 atttctcacg gtaaatgtat cgctgaattt tacccgtttt tccgttcagg atgtggtttt
     2281 ctgacgcgct gcgcttgtca aaccggcgac ggccacgcaa tgaattgcgc ccccgtcatc
     2341 ggttattaga ggctgtctgc ccccggatac ccggattctt tctgatgcct gacggtgacg
     2401 atcacaatct ggtccatttc ccggtcgtgg cggtagagca tcacatagcc actgtcgcca
     2461 aacccgatca ccagttcctt atattccagc ggcagaaacg gcaccgggcg gccaatgtct
     2521 ggcaatgttt tcagttgctg gatagcccgg acgatcacct cacctccttt tctggctgcc
     2581 agccgatttt tggttttgag aaagtcctga aggcgttgta aatcgcgctg tgccagcgcg
     2641 gaaatcgtta cctgtggcat ggcggagcat cctgctcgtt ctcagttccc caggtactta
     2701 tccaggcttc cgcttcttcc gcagtaaggt gcagacccgt ttcctgatag tgctgccatg
     2761 cggcctgtcc gtcccgcagg tactggctgc gcttctcttc ccggtcgatg tattcggtga
     2821 ttgcctccag cattagcgcg tgcgctgagc ggtgccggtc atcggccagc gttttcagcc
     2881 ggtccttaag ttcttcatcg agcctgatgg atgttgctga tgacataaaa gcccctttgt
     2941 agtcatatgt attacacatg actactttaa tccgtggcgg cgctatttgc cagaaatacg
     3001 gcttttgctg cccatttttc agcttcgctt ccactaatca gggctgctac aggctcactc
     3061 atcatccagg actattgcag cctgcgggtt aaaggacctg ttactggcgg caaccatcat
     3121 cattgagctt ttgggtggtg actggtcgcg gatcctgtaa gccgtcattt ttcagactga
     3181 attcaaaatt tataacctgt cgcattgccg ggtgccctgc gggatatagc cagtgcctca
     3241 ttgccgtcag cgtttcccga aaaaaaaggt gacagccggt aagcgacacg ttgtccactc
     3301 gtccatcgtc cagaatatca aacgtggcgg tcacctgacc ttcgatgcgt tttgctgcgg
     3361 cctgtaccgg gtaggttggc tgcagtgtgg ttacgggttt tagtggtatc gcatgtaccg
     3421 gagtgaggaa catggccgcc agcactgtca gagtaaaagc tttcatggtc tgaaatccag
     3481 aggggcgttt ttgaatattt gccaggtatt ttcatccggt gccggcggaa ttttggcgtg
     3541 ggtaacggct gatattgctg ctttacagag tttcggatct cctccttcag caatggcgct
     3601 gttcagagtg ccatcacgtt gtatggatat ccgcacggtg cacatttttc ccgcataggc
     3661 ctctggttca aacatctgca tttcaacagc ggtcttgatg gccgcagcgt agctactgat
     3721 tttagatgac ttatccagag tataaatttt cggcggctgg gtggtggaat gcttacaggc
     3781 cgtcaggagt aaggggctgc acaatgccag caggataaag ggtattaaac gcatcgggtt
     3841 gatgacctgt cagtaaaaaa tgttacatgt atatttgtca ctctttacgg ccacgttcat
     3901 ttacataaac acggcttatc gtatcgtttt tacaccaaac ggcccgccat gcagttgcgt
     3961 gcataggcat gcagcgggtt acaatttacc gggcgcgttt ccggcggttt tccgggtggt
     4021 ttgttgcctg ttttatcccg tagccgccgg aaacgccctc agaccgtctg agcggtgcgg
     4081 gcaatgcgac gttatggtaa atacccctta ccggcggcgc tgtggctgcc aggtgaaggt
     4141 ggtttccgta gcctcgtatt tctcccgacg catttcctgc gcccgcctgg cttcacgttc
     4201 gtcctgaatg gcacgaagca ccctttcccg cacctgttgt tttaccgcct ttggcagcgt
     4261 tttgttccct ttcaggccca tggccagtgc ctgagcgaca aaggcgtccg ccaggcgctt
     4321 ttcttcagcg ggagtcagtt tcaggtgcgt catagtggcc tccgggcgtc tcaggtttcc
     4381 cgaacagttt aaccgcaggt cctctcctca tgccagttag ctttcagatg tgtctatacc
     4441 atctgaaatg taagtgtgat gaatgtctta aataataaaa tttgttgcca tgagaaattt
     4501 tcgggaaaag acgcgatctg cttacaggtg gctccgggcg cttttttgtg cctgaagatg
     4561 agaactaatc tcatatattg acacctgaaa acgggaggag taaggtaata attatactgt
     4621 gtatatgtac agtatgttgg ctttaattta ttgttttaaa tgtcaaagag gaaaacggta
     4681 tgagtggtgg agacggtcga ggtccgggta attcaggtct gggacataat ggtggtcagg
     4741 ccagtgggaa tgtgaacggt acgtctggta aaggtggccc ttcatcaggt ggtggtacgg
     4801 atccaaacag cgggccgggc tggggtacga cgcatacgcc gaatggagat attcataact
     4861 ataatccggg ggagtttggt aacggcggga gtaaacccgg tggtaatggt ggtaacagcg
     4921 gcaatcatag cggtagctct ggtggcggac agtcttcggc cactgcgatg gccttcggtc
     4981 tgcctgccct ggctactccg ggggctgaag gactggcttt atcagtttcc ggcgatgcgt
     5041 tgtcggccgc cgttgctgat gtgctggctg ccctgaaggg gccgtttaag tttggtctgt
     5101 gggggattgc gatctacggt gtgctgcctt ctgagattgc aaaagatgat ccgaaaatga
     5161 tgtcaaaaat tatgacgtca ttaccggccg atgcggtgac ggagactccg gcaagtactt
     5221 taccactgga ccaggcgacg gttcgtgtca gacaacgggt tgtggatgtg gtgaaggatg
     5281 agcggcagca tattgcggtt gtcgcaggtc ggccaatgag tgtccctgtg gtggatgcga
     5341 aaccgacaaa acgtccgggg gtattcagtg tgtcgattcc gggtctcccg tctctgcagg
     5401 tgagcgtacc gaaaggtgtt ccgacagcga aagccccgcc aaaaggcatt gttgctgaaa
     5461 aaggtgattc acgtccggct ggttttacag ccggtggtaa ctcccgtgag gccgttattc
     5521 gtttcccgaa agagaccgga cagaagccgg tttatgtgtc ggtgacagat gttcttaccc
     5581 cggcacaggt aaaacagcgt caggaggaag aaaagcgtcg ccagcaggca tgggacgccg
     5641 ctcatccgga agaggggctg aaaagagact atgataaagc gaaagctgag ctggatgccg
     5701 aagataaaaa tattgcgacc ttaaacagcc gcattgcatc gacagagaag gcgctccccg
     5761 gtgcaagggc tgctgttcag gaagccgata aaaaggtaaa agaggcagag gcaaataaag
     5821 atgattttgt gacttataac ccgcctcatg aatatggctc cgggtggcag gatcaggttc
     5881 gctatcttga taaggatatt cagaatcaga atgagaaatt aaaagcggct caggcatctt
     5941 taaacgcaat gaatgaatcc ttatccagag ataaggctgc acttaccggg gcgatggaga
     6001 gccggaaaca aaaggagaaa aaagcgaagg atgcagaaaa taagttaaat gaggaaaaga
     6061 acaagcctcg caaggggact aaagactacg gccatgatta tcatccagta cctaaaacag
     6121 aagacataaa gggacttgga gagttaaagc gaggtgatcc taaaacacct aaacaaggtg
     6181 gtggtggtaa gcgggcccga tggtatggcg ataaaaaacg taaaatttat gaatgggatt
     6241 cccagcacgg tgagcttgaa gggtatcgtg ccagtgatgg cgaacaccta ggcgcatttg
     6301 atcccaaaac gggtaagcag gttaaagggc cagatccaaa acggaacatt aaaaaatatc
     6361 tttgagaggt aaatatgggg cttaaattac gtttgacatg gtttgataag aaaactgaag
     6421 agtttaaagg ggaagagtat tctaaagact ttggtgacga tggttctgtc attgaaagct
     6481 tgggaatgcc tttaaaggat aatattaata atggtgagtt tgatttgaag aaagaatggg
     6541 ttcctttact acagccattt tttaaaaatc aaataaaacc tgatgtggat aattgttata
     6601 tagcttttga ttatcgggac ggtaactggt aacaaaaaat tatcaaggat gatttgaaat
     6661 actgacttcc ggtgttcagg gatgaatgcc ggagtcttta aatacaggga aagtatatga
     6721 aaaaggtaaa gactattttt ctttttatat tgattgttgc tggctttctt ctggccgcat
     6781 gtcaggcaaa ttatatccgg gatgttcagg gcggaacggt ggcaccatcg tcctcctctg
     6841 aactgacggg gatcgcggtt cagtagaaaa gatcaaagga tcttcttgag atcctttttt
     6901 tctgcgcgta atcttttgcc ctgtaaacga aaaaaccacc tggggaggtg gtttgatcga
     6961 aggttaagtc agttggggaa ctgcttaacc gtggtaactg gctttcgcag agcacagcaa
     7021 ccaaatctgt ccttccagtg tagccggact ttggcgcaca cttcaagagc aaccgcgtgt
     7081 ttagctaaac aaatcctctg cgaactccca gttaccaatg gctgctgcca gtggcgtttt
     7141 gcgtgctttc ccgggttgga ctcaagtgaa cagttaccgg ataaggcgca gcagtcgggc
     7201 tgaacgggga gttcttgctt acagcccagc ttggagcgaa cgacctacac cgagccgaga
     7261 taccagtgtg tgagctatga gaaagcgcca cacttcccgc aagggagaac ggcggacagg
     7321 tatccggtaa acggcagggt cggaacagga gagcgcaaga gggagcgacc taccggaaac
     7381 ggtggggatc tttaagtcct gtcgggtttc gcccgtactg tcagattcat ggttgagcct
     7441 cacggctccc acagatgcac cgaaaaagcg tctgtttatg tgaactctgg caggagggcg
     7501 gagcctatgg aaaaacgcca ccggcgcggc cctgccgttt tgcctcacat gttagtcccc
     7561 tgcttatcca cggaatctgt gggtaacttt gtatgtgtcc gcagcgcccg ccgcagtctc
     7621 acgcccggag cgtagcgacc gagtgagcga ggaggcgcaa tttccccggc acctgcaaga
     7681 cggtcaccgg acgcgctgat gtgggatatt gtcccctgat taagtctttc tattccctga
     7741 cgatcaatta tcaggatagg tgaaccatgt cggtttttcg ctgaacattt tacgttaacc
     7801 tatctctgtt gcttttttct ccttgcggag aaatgcaagt catccccgct ggggcgtggc
     7861 tgacttcagg ggaaaagacc ttgcttacgc tggctggacg ccaccgacgc acggtctttt
     7921 cccctctttc tggctctgtc cggctggacg ccggccacgc cagaaattca cccggccccc
     7981 gtcaggctca cgcctgacca cccacaaaaa tccacggtgc tggacgcacc tgacgaagca
     8041 gcaaagctgc tcctgatttt gcgggtggtc agccgctcgc tatggcgaac tctgacgggg
     8101 gccggaacac cggaaaagac cgcactgggg tgccgccttt tcctctcaca cgctggggcg
     8161 tgctcgttgt tcctccgggg ctgcgccttt tccgcatggg gcggaaagag gctggcctcc
     8221 gcggtgaaag ctgaacgcaa tcaccacgga cgtgaccgcc gccgccatgc cttggggcat
     8281 gtccgcggct tgtgcagcgg gggctgcgca tcacgccggt aaacccgtcg tctgaaggag
     8341 agagattgca tgtttaatac ggattcgctt gcctggcagt ggcatatccc ggagctggcc
     8401 cggcagattt acccgtgggt cagctggctg taccgggagt attacagtct ggccggtttt
     8461 tttctggtta tcggcctgtt tttcggtctg gctgaactct gggccggccg aatgcgtttt
     8521 gtggggctga gccataaggc gcggcgggag tatgccgatc gctataagcg cgataataac
     8581 cggactgtgc cattttcggt gctggcctcg ctggattcac ccgggcggat ggtcagggca
     8641 cggcgacatg tctggaacta cgcgtactgg ctgaccggcg ctgccggtct ggcccttctg
     8701 ggggggctgg ttgtcgccgt gtttatcgag agctggggag aaaccgtcct gcgcccggac
     8761 ctctatgcga acgcagatta ttacctgctg cagccggcta acctgttcgg gatagcctgg
     8821 tgtgccggag ggcttgtcgc cggcgtcctt ctggcgcggc tgctggcgtt cctgctgctt
     8881 gatggtcatt ttgcggccgc tgacgaggcg gtgaacgcga aactgaatca ggagagccgg
     8941 cgcagcagcc agcggaccgg agaaatgaca gacgtccgcc acctgcattt cggggagccg
     9001 gtaccggtta acgcgctggc cgatttttcc acggagcagg cccggaaaca gcaggcggtg
     9061 tttctgggta aggatgagca gggccagccg gtgctggtcc ctcgtgatac ctggcgcaag
     9121 accaatattc agatcctcgg cctgcccggg agcggcaaga gcgcgatggc caccaacgcg
     9181 ctcattcgct gcgtacgcga tttcggggac gctgtggtgt acttcgaccc caaaggggac
     9241 gcgtgggcgc cgcacgtctt ccgggcgcac tgcccggact tcaccctgct ggatctgcgg
     9301 ccggggaaac cggcgcagct gaatctgttc cgcgatctcg accagtacgc cctgaagaat
     9361 ctgctggttg ccgggtttaa cctgagtgag accggcgacg tggcggacca ctaccgcatc
     9421 agcgagcaga aagcggcgaa gctgattgcg gagcagttcc cgcagggggc aaacattcag
     9481 caggtgctgg cggcggcgta cgcgttgccg gaggccctga aaaaggacgt gaaggggctg
     9541 atcaccaagc tggagaacgt ggccgacctg agcgtcctgc agacggacag cggtatcgat
     9601 gtggcgggga taatcaacgg cggcggctgc ctgtacgtca tcggctcgat ggacgatgag
     9661 gcggtgatcc gggtgcagaa gatgctgttt gcccgctgcg cgcaaatcat cattgcgcgg
     9721 gatgagttcc ggaggtggcc gcacgcgagc atcatgctcg atgagattaa atatctgctg
     9781 tcgaagtacg tgctgaacgc gctggggacg ctgcgcagcc gggactgcaa cctgctgctc
     9841 gcgcaccagt cgctgggcga cttcggtcag tgcgggcagg atctgcccgc tgattttgtg
     9901 aaaaccacgg tactggataa cacgccgatc cgctggttct atcgcgcggc cagccaggag
     9961 tccgcgcagt gggcggcggg ccagaccggg gagatccggg tggacgtgga gcgccgccgg
    10021 gccagccggg aggcggggaa cgtggagcac atcagcggcg
//
