LOCUS       NZ_CP043346            10060 bp    DNA     circular CON 30-MAY-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain WCHKP020039 plasmid
            p1_020039, complete sequence.
ACCESSION   NZ_CP043346
VERSION     NZ_CP043346.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN08022162
            Assembly: GCF_002894385.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Liu,L., Hu,Y., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Klebsiella pneumoniae strain WCHKP020039
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Liu,L., Hu,Y., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2019) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
REFERENCE   3  (bases 1 to 10060)
  AUTHORS   Liu,L., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-NOV-2017) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002894385.2-RS_2024_05_30
            Annotation Date                   :: 05/30/2024 04:10:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,510
            CDSs (total)                      :: 5,391
            Genes (coding)                    :: 5,260
            CDSs (with protein)               :: 5,260
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 47 of 131
            Pseudo Genes (incomplete)         :: 84 of 131
            Pseudo Genes (internal stop)      :: 15 of 131
            Pseudo Genes (multiple problems)  :: 15 of 131
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP043346.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WCHKP020039"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="p1_020039"
                     /geo_loc_name="China: Sichuan, Chengdu"
                     /collection_date="2016-11-07"
     gene            join(9332..10060,1..3)
                     /gene="mobC"
                     /locus_tag="CVC44_RS27320"
                     /old_locus_tag="CVC44_27715"
     CDS             join(9332..10060,1..3)
                     /gene="mobC"
                     /locus_tag="CVC44_RS27320"
                     /old_locus_tag="CVC44_27715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            10..540
                     /locus_tag="CVC44_RS27325"
                     /old_locus_tag="CVC44_27720"
     CDS             10..540
                     /locus_tag="CVC44_RS27325"
                     /old_locus_tag="CVC44_27720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(567..746)
                     /locus_tag="CVC44_RS27330"
                     /old_locus_tag="CVC44_27725"
     CDS             complement(567..746)
                     /locus_tag="CVC44_RS27330"
                     /old_locus_tag="CVC44_27725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(772..1200)
                     /locus_tag="CVC44_RS27335"
                     /old_locus_tag="CVC44_27730"
     CDS             complement(772..1200)
                     /locus_tag="CVC44_RS27335"
                     /old_locus_tag="CVC44_27730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(1364..1678)
                     /locus_tag="CVC44_RS27340"
                     /old_locus_tag="CVC44_27735"
     CDS             complement(1364..1678)
                     /locus_tag="CVC44_RS27340"
                     /old_locus_tag="CVC44_27735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1666..1944)
                     /locus_tag="CVC44_RS27345"
                     /old_locus_tag="CVC44_27740"
     CDS             complement(1666..1944)
                     /locus_tag="CVC44_RS27345"
                     /old_locus_tag="CVC44_27740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(2119..2484)
                     /locus_tag="CVC44_RS27350"
                     /old_locus_tag="CVC44_27745"
     CDS             complement(2119..2484)
                     /locus_tag="CVC44_RS27350"
                     /old_locus_tag="CVC44_27745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(2481..2852)
                     /gene="tolA"
                     /locus_tag="CVC44_RS27355"
                     /old_locus_tag="CVC44_27750"
     CDS             complement(2481..2852)
                     /gene="tolA"
                     /locus_tag="CVC44_RS27355"
                     /old_locus_tag="CVC44_27750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(3126..3371)
                     /locus_tag="CVC44_RS27360"
                     /old_locus_tag="CVC44_27755"
     CDS             complement(3126..3371)
                     /locus_tag="CVC44_RS27360"
                     /old_locus_tag="CVC44_27755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            3698..5383
                     /locus_tag="CVC44_RS27365"
                     /old_locus_tag="CVC44_27760"
     CDS             3698..5383
                     /locus_tag="CVC44_RS27365"
                     /old_locus_tag="CVC44_27760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            5393..5650
                     /locus_tag="CVC44_RS27370"
                     /old_locus_tag="CVC44_27765"
     CDS             5393..5650
                     /locus_tag="CVC44_RS27370"
                     /old_locus_tag="CVC44_27765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            5735..5884
                     /locus_tag="CVC44_RS27375"
                     /old_locus_tag="CVC44_27770"
     CDS             5735..5884
                     /locus_tag="CVC44_RS27375"
                     /old_locus_tag="CVC44_27770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            7368..9332
                     /locus_tag="CVC44_RS27380"
                     /old_locus_tag="CVC44_27780"
     CDS             7368..9332
                     /locus_tag="CVC44_RS27380"
                     /old_locus_tag="CVC44_27780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     BGC             1..10060
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(5988..6566)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          9333..10060
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 tgagggatta tggcgctgta catggtattt attgaggacg tggaagacaa agacggcgag
       61 cgctgcatta tgccaaccag cgagcatctg gaagacatgg tggtgctcgc ctggcagacc
      121 ggggcgtatg ttttctgttt tgtcgaccgg gcccagcatc tggcgatgat gaaatccatc
      181 gggatgtatg acgagcggct caacagcgac gaaaaagcca cgtacttttt caccggtgaa
      241 aatccgcaat gggaacggga caaacccaca ggaatgaagc tgtgtgatta cctctatcga
      301 aaggtggagc ggttcgccat cgagaacggt gtgcaggtgg ttgatgccgg gaagtgctca
      361 ccggagcaga ttaacgcgat gattgaggtg gtcagcgagc ggcacgggga aagcgcggtg
      421 aacgagaagc tgaaagcgga gctgatgcgg gaatataaca gccgctattt taacgtgccg
      481 gtgcctgaac tgccggagga caaacccgcc cgccggtggt ggccgttcgg caaaaaataa
      541 cgccagcacc ggcatggccg gtgctgttat tcttccccga gcttatcccg catcacctgc
      601 cagagtgttt ccgcatcctc gtgaagccgt tcgcacatgt cggccggggt atccatgtcc
      661 agctcgttca gcttttccag caacagcagc gactgatcct taatgaattt agccatattg
      721 agggcagttt tttcctgctt cgtcatgttc tccattccct tcggtaagtt ctcagctaaa
      781 ccagccggac cagaagcgtt ttttcttctg tgtcagttct gttgtcaatc tgtccacttc
      841 ggcctggagt ttccggcgct catcatcgtg ctggcggcgt tcttcttccc gggatgtttt
      901 atcttccagc atcagggtga cagcctgccg gagatcggcc agttcctttt gcattgccag
      961 aagctgttcc tgtgggaaat ttttttgtgt attttcacgg tgtgaaatgc cgggaatttg
     1021 tttctcaggg atattcacat ttccgtatac acggatcagt tctgacacat ccacgacagg
     1081 gttgtttttc ccgtcacgtg acacggtaac ctttccctgt tttacatgat tgtatagcgt
     1141 tcttctggta atgcccgcgg cttgtgctgc ctgtgaaagg ttgagcaatg ttttcgccac
     1201 tgtatacccc tgattttatc gggtgtgaaa ggtgtgtaat ttctcacggt aaatgtatcg
     1261 ctgaatttta cccgtttttc cgttcaggat gtggttttct gacgcgctgc gcttgtcaaa
     1321 ccggcgacgg ccacgcaatg aattgcgccc ccgtcatcgg ttattagagg ctgtctgccc
     1381 ccggataccc ggattctttc tgatgcctga cggtgacgat cacaatctgg tccatttccc
     1441 ggtcgtggcg gtagagcatc acatagccac tgtcgccaaa cccgatcacc agttccttat
     1501 attccagcgg cagaaacggc accgggcggc caatgtctgg caatgttttc agttgctgga
     1561 tagcccggac gatcacctca cctccttttc tggctgccag ccgatttttg gttttgagaa
     1621 agtcctgaag gcgttgtaaa tcgcgctgtg ccagcgcgga aatcgttacc tgtggcatgg
     1681 cggagcatcc tgctcgttct cagttcccca ggtacttatc caggcttccg cttcttccgc
     1741 agtaaggtgc agacccgttt cctgatagtg ctgccatgcg gcctgtccgt cccgcaggta
     1801 ctggctgcgc ttctcttccc ggtcgatgta ttcggtgatt gcctccagca ttagcgcgtg
     1861 cgctgagcgg tgccggtcat cggccagcgt tttcagccgg tccttaagtt cttcatcgag
     1921 cctgatggat gttgctgatg acataaaagc ccctttgtag tcatatgtat tacacatgac
     1981 tactttaatc cgtggcggcg ctatttgcca gaaatacggc ttttgctgcc catttttcag
     2041 cttcgcttcc actaatcagg gctgctacag gctcactcat catccaggac tattgcagcc
     2101 tgcgggttaa aggacctgtt actggcggca accatcatca ttgagctttt gggtggtgac
     2161 tggtcgcgga tcctgtaagc cgtcattttt cagactgaat tcaaaattta taacctgtcg
     2221 cattgccggg tgccctgcgg gatatagcca gtgcctcatt gccgtcagcg tttcccgaaa
     2281 aaaaaggtga cagccggtaa gcgacacgtt gtccactcgt ccatcgtcca gaatatcaaa
     2341 cgtggcggtc acctgacctt cgatgcgttt tgctgcggcc tgtaccgggt aggttggctg
     2401 cagtgtggtt acgggtttta gtggtatcgc atgtaccgga gtgaggaaca tggccgccag
     2461 cactgtcaga gtaaaagctt tcatggtctg aaatccagag gggcgttttt gaatatttgc
     2521 caggtatttt catccggtgc cggcggaatt ttggcgtggg taacggctga tattgctgct
     2581 ttacagagtt tcggatctcc tccttcagca atggcgctgt tcagagtgcc atcacgttgt
     2641 atggatatcc gcacggtgca catttttccc gcataggcct ctggttcaaa catctgcatt
     2701 tcaacagcgg tcttgatggc cgcagcgtag ctactgattt tagatgactt atccagagta
     2761 taaattttcg gcggctgggt ggtggaatgc ttacaggccg tcaggagtaa ggggctgcac
     2821 aatgccagca ggataaaggg tattaaacgc atcgggttga tgacctgtca gtaaaaaatg
     2881 ttacatgtat atttgtcact ctttacggcc acgttcattt acataaacac ggcttatcgt
     2941 atcgttttta caccaaacgg cccgccatgc agttgcgtgc ataggcatgc agcgggttac
     3001 aatttaccgg gcgcgtttcc ggcggttttc cgggtggttt gttgcctgtt ttatcccgta
     3061 gccgccggaa acgccctcag accgtctgag cggtgcgggc aatgcgacgt tatggtaaat
     3121 accccttacc ggcggcgctg tggctgccag gtgaaggtgg tttccgtagc ctcgtatttc
     3181 tcccgacgca tttcctgcgc ccgcctggct tcacgttcgt cctgaatggc acgaagcacc
     3241 ctttcccgca cctgttgttt taccgccttt ggcagcgttt tgttcccttt caggcccatg
     3301 gccagtgcct gagcgacaaa ggcgtccgcc aggcgctttt cttcagcggg agtcagtttc
     3361 aggtgcgtca tagtggcctc cgggcgtctc aggtttcccg aacagtttaa ccgcaggtcc
     3421 tctcctcatg ccagttagct ttcagatgtg tctataccat ctgaaatgta agtgtgatga
     3481 atgtcttaaa taataaaatt tgttgccatg agaaattttc gggaaaagac gcgatctgct
     3541 tacaggtggc tccgggcgct tttttgtgcc tgaagatgag aactaatctc atatattgac
     3601 acctgaaaac gggaggagta aggtaataat tatactgtgt atatgtacag tatgttggct
     3661 ttaatttatt gttttaaatg tcaaagagga aaacggtatg agtggtggag acggtcgagg
     3721 tccgggtaat tcaggtctgg gacataatgg tggtcaggcc agtgggaatg tgaacggtac
     3781 gtctggtaaa ggtggccctt catcaggtgg tggtacggat ccaaacagcg ggccgggctg
     3841 gggtacgacg catacgccga atggagatat tcataactat aatccggggg agtttggtaa
     3901 cggcgggagt aaacccggtg gtaatggtgg taacagcggc aatcatagcg gtagctctgg
     3961 tggcggacag tcttcggcca ctgcgatggc cttcggtctg cctgccctgg ctactccggg
     4021 ggctgaagga ctggctttat cagtttccgg cgatgcgttg tcggccgccg ttgctgatgt
     4081 gctggctgcc ctgaaggggc cgtttaagtt tggtctgtgg gggattgcga tctacggtgt
     4141 gctgccttct gagattgcaa aagatgatcc gaaaatgatg tcaaaaatta tgacgtcatt
     4201 accggccgat gcggtgacgg agactccggc aagtacttta ccactggacc aggcgacggt
     4261 tcgtgtcaga caacgggttg tggatgtggt gaaggatgag cggcagcata ttgcggttgt
     4321 cgcaggtcgg ccaatgagtg tccctgtggt ggatgcgaaa ccgacaaaac gtccgggggt
     4381 attcagtgtg tcgattccgg gtctcccgtc tctgcaggtg agcgtaccga aaggtgttcc
     4441 gacagcgaaa gccccgccaa aaggcattgt tgctgaaaaa ggtgattcac gtccggctgg
     4501 ttttacagcc ggtggtaact cccgtgaggc cgttattcgt ttcccgaaag agaccggaca
     4561 gaagccggtt tatgtgtcgg tgacagatgt tcttaccccg gcacaggtaa aacagcgtca
     4621 ggaggaagaa aagcgtcgcc agcaggcatg ggacgccgct catccggaag aggggctgaa
     4681 aagagactat gataaagcga aagctgagct ggatgccgaa gataaaaata ttgcgacctt
     4741 aaacagccgc attgcatcga cagagaaggc gctccccggt gcaagggctg ctgttcagga
     4801 agccgataaa aaggtaaaag aggcagaggc aaataaagat gattttgtga cttataaccc
     4861 gcctcatgaa tatggctccg ggtggcagga tcaggttcgc tatcttgata aggatattca
     4921 gaatcagaat gagaaattaa aagcggctca ggcatcttta aacgcaatga atgaatcctt
     4981 atccagagat aaggctgcac ttaccggggc gatggagagc cggaaacaaa aggagaaaaa
     5041 agcgaaggat gcagaaaata agttaaatga ggaaaagaac aagcctcgca aggggactaa
     5101 agactacggc catgattatc atccagtacc taaaacagaa gacataaagg gacttggaga
     5161 gttaaagcga ggtgatccta aaacacctaa acaaggtggt ggtggtaagc gggcccgatg
     5221 gtatggcgat aaaaaacgta aaatttatga atgggattcc cagcacggtg agcttgaagg
     5281 gtatcgtgcc agtgatggcg aacacctagg cgcatttgat cccaaaacgg gtaagcaggt
     5341 taaagggcca gatccaaaac ggaacattaa aaaatatctt tgagaggtaa atatggggct
     5401 taaattacgt ttgacatggt ttgataagaa aactgaagag tttaaagggg aagagtattc
     5461 taaagacttt ggtgacgatg gttctgtcat tgaaagcttg ggaatgcctt taaaggataa
     5521 tattaataat ggtgagtttg atttgaagaa agaatgggtt cctttactac agccattttt
     5581 taaaaatcaa ataaaacctg atgtggataa ttgttatata gcttttgatt atcgggacgg
     5641 taactggtaa caaaaaatta tcaaggatga tttgaaatac tgacttccgg tgttcaggga
     5701 tgaatgccgg agtctttaaa tacagggaaa gtatatgaaa aaggtaaaga ctatttttct
     5761 ttttatattg attgttgctg gctttcttct ggccgcatgt caggcaaatt atatccggga
     5821 tgttcagggc ggaacggtgg caccatcgtc ctcctctgaa ctgacgggga tcgcggttca
     5881 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat cttttgccct
     5941 gtaaacgaaa aaaccacctg gggaggtggt ttgatcgaag gttaagtcag ttggggaact
     6001 gcttaaccgt ggtaactggc tttcgcagag cacagcaacc aaatctgtcc ttccagtgta
     6061 gccggacttt ggcgcacact tcaagagcaa ccgcgtgttt agctaaacaa atcctctgcg
     6121 aactcccagt taccaatggc tgctgccagt ggcgttttgc gtgctttccc gggttggact
     6181 caagtgaaca gttaccggat aaggcgcagc agtcgggctg aacggggagt tcttgcttac
     6241 agcccagctt ggagcgaacg acctacaccg agccgagata ccagtgtgtg agctatgaga
     6301 aagcgccaca cttcccgcaa gggagaacgg cggacaggta tccggtaaac ggcagggtcg
     6361 gaacaggaga gcgcaagagg gagcgaccta ccggaaacgg tggggatctt taagtcctgt
     6421 cgggtttcgc ccgtactgtc agattcatgg ttgagcctca cggctcccac agatgcaccg
     6481 aaaaagcgtc tgtttatgtg aactctggca ggagggcgga gcctatggaa aaacgccacc
     6541 ggcgcggccc tgccgttttg cctcacatgt tagtcccctg cttatccacg gaatctgtgg
     6601 gtaactttgt atgtgtccgc agcgcccgcc gcagtctcac gcccggagcg tagcgaccga
     6661 gtgagcgagg aggcgcaatt tccccggcac ctgcaagacg gtcaccggac gcgctgatgt
     6721 gggatattgt cccctgatta agtctttcta ttccctgacg atcaattatc aggataggtg
     6781 aaccatgtcg gtttttcgct gaacatttta cgttaaccta tctctgttgc ttttttctcc
     6841 ttgcggagaa atgcaagtca tccccgctgg ggcgtggctg acttcagggg aaaagacctt
     6901 gcttacgctg gctggacgcc accgacgcac ggtcttttcc cctctttctg gctctgtccg
     6961 gctggacgcc ggccacgcca gaaattcacc cggcccccgt caggctcacg cctgaccacc
     7021 cacaaaaatc cacggtgctg gacgcacctg acgaagcagc aaagctgctc ctgattttgc
     7081 gggtggtcag ccgctcgcta tggcgaactc tgacgggggc cggaacaccg gaaaagaccg
     7141 cactggggtg ccgccttttc ctctcacacg ctggggcgtg ctcgttgttc ctccggggct
     7201 gcgccttttc cgcatggggc ggaaagaggc tggcctccgc ggtgaaagct gaacgcaatc
     7261 accacggacg tgaccgccgc cgccatgcct tggggcatgt ccgcggcttg tgcagcgggg
     7321 gctgcgcatc acgccggtaa acccgtcgtc tgaaggagag agattgcatg tttaatacgg
     7381 attcgcttgc ctggcagtgg catatcccgg agctggcccg gcagatttac ccgtgggtca
     7441 gctggctgta ccgggagtat tacagtctgg ccggtttttt tctggttatc ggcctgtttt
     7501 tcggtctggc tgaactctgg gccggccgaa tgcgttttgt ggggctgagc cataaggcgc
     7561 ggcgggagta tgccgatcgc tataagcgcg ataataaccg gactgtgcca ttttcggtgc
     7621 tggcctcgct ggattcaccc gggcggatgg tcagggcacg gcgacatgtc tggaactacg
     7681 cgtactggct gaccggcgct gccggtctgg cccttctggg ggggctggtt gtcgccgtgt
     7741 ttatcgagag ctggggagaa accgtcctgc gcccggacct ctatgcgaac gcagattatt
     7801 acctgctgca gccggctaac ctgttcggga tagcctggtg tgccggaggg cttgtcgccg
     7861 gcgtccttct ggcgcggctg ctggcgttcc tgctgcttga tggtcatttt gcggccgctg
     7921 acgaggcggt gaacgcgaaa ctgaatcagg agagccggcg cagcagccag cggaccggag
     7981 aaatgacaga cgtccgccac ctgcatttcg gggagccggt accggttaac gcgctggccg
     8041 atttttccac ggagcaggcc cggaaacagc aggcggtgtt tctgggtaag gatgagcagg
     8101 gccagccggt gctggtccct cgtgatacct ggcgcaagac caatattcag atcctcggcc
     8161 tgcccgggag cggcaagagc gcgatggcca ccaacgcgct cattcgctgc gtacgcgatt
     8221 tcggggacgc tgtggtgtac ttcgacccca aaggggacgc gtgggcgccg cacgtcttcc
     8281 gggcgcactg cccggacttc accctgctgg atctgcggcc ggggaaaccg gcgcagctga
     8341 atctgttccg cgatctcgac cagtacgccc tgaagaatct gctggttgcc gggtttaacc
     8401 tgagtgagac cggcgacgtg gcggaccact accgcatcag cgagcagaaa gcggcgaagc
     8461 tgattgcgga gcagttcccg cagggggcaa acattcagca ggtgctggcg gcggcgtacg
     8521 cgttgccgga ggccctgaaa aaggacgtga aggggctgat caccaagctg gagaacgtgg
     8581 ccgacctgag cgtcctgcag acggacagcg gtatcgatgt ggcggggata atcaacggcg
     8641 gcggctgcct gtacgtcatc ggctcgatgg acgatgaggc ggtgatccgg gtgcagaaga
     8701 tgctgtttgc ccgctgcgcg caaatcatca ttgcgcggga tgagttccgg aggtggccgc
     8761 acgcgagcat catgctcgat gagattaaat atctgctgtc gaagtacgtg ctgaacgcgc
     8821 tggggacgct gcgcagccgg gactgcaacc tgctgctcgc gcaccagtcg ctgggcgact
     8881 tcggtcagtg cgggcaggat ctgcccgctg attttgtgaa aaccacggta ctggataaca
     8941 cgccgatccg ctggttctat cgcgcggcca gccaggagtc cgcgcagtgg gcggcgggcc
     9001 agaccgggga gatccgggtg gacgtggagc gccgccgggc cagccgggag gcggggaacg
     9061 tggagcacat cagcggcgac agcttcatcc agaaggaggc gcggccgctg tttgacgtga
     9121 acaccctgca acacctgcct gacgggttcg cggtgatgac cgggctgggc gtggcgcggc
     9181 tggcgttcag cagcccgctg cgggttgagc gccgggaaat ttcgctgaaa accttcccgg
     9241 tgctggcgaa aacggacccg ctggcggagt acctgcctcc ggccgacggc ggcggaccaa
     9301 aagcggatga tgattttgcg gggctttact gatgacactg gagcagtaca acgtcagcca
     9361 cgcgaagcgg caggcgcgga acgcggagaa aacacggctg accctgcgct ggctgcggga
     9421 agagctgtgt tccacggcgg agctggttgc gcgacggctg gggattgcgg cggtacagcc
     9481 ggtttaccgc ttcctggaca gtctggtggc gaaagggctg ctggtccggg cgaaataccc
     9541 ggttgacggc cgtcaggtca gcgtgtgggg gctgacgccg cacggggtgg cgttctcgtt
     9601 cgacgaggac gagccgctga cggacgttat tccgtttcag ccgtcaaggg tgtcggcggc
     9661 gcagctgccg caccggctgg ccgtgcagtc cctgcgcctc tccatggagg gccggggggc
     9721 caccggctgg cggtacctgc accgtatggc cctgaaaggg atgaaagtgc cggacgcgct
     9781 ggcggagctg gacggcagaa cggtggcatt tgaggtagag cgcaccgtca agagccgcag
     9841 gcggtatcag gaagtggtgt ccgggtatct gttcaaccgc cgggcgaacg gcatcgatga
     9901 aatctggtac atctgccctg accggccgac gcttgtccgg gtacagcggg cgattctggc
     9961 ggtggatgaa atcgtgaatc cacagaccgg cgaggcccgc aaaacggtgg agctggaccg
    10021 ggagaggctg ttcgcgtgct ttaaatttat gacaacggag
//
