LOCUS       NZ_CP043340             2593 bp    DNA     circular CON 24-MAY-2024
DEFINITION  Escherichia coli strain WCHEC115106 plasmid p5_115106, complete
            sequence.
ACCESSION   NZ_CP043340
VERSION     NZ_CP043340.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12623214
            Assembly: GCF_022559065.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Hu,Y., Long,H., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Escherichia coli strain WCHEC115106
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Hu,Y., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2019) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022559065.1-RS_2024_05_24
            Annotation Date                   :: 05/24/2024 04:21:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,139
            CDSs (total)                      :: 5,017
            Genes (coding)                    :: 4,754
            CDSs (with protein)               :: 4,754
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 87 of 263
            Pseudo Genes (incomplete)         :: 171 of 263
            Pseudo Genes (internal stop)      :: 54 of 263
            Pseudo Genes (multiple problems)  :: 41 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP043340.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="WCHEC115106"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p5_115106"
                     /geo_loc_name="China: Chengdu, Sichuan"
                     /collection_date="2017"
     gene            complement(31..144)
                     /locus_tag="FZC46_RS25550"
                     /old_locus_tag="FZC46_25850"
     CDS             complement(31..144)
                     /locus_tag="FZC46_RS25550"
                     /old_locus_tag="FZC46_25850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072206721.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKAPLAAATGN"
                     /name="replication initiation protein"
     gene            <460..642
                     /locus_tag="FZC46_RS25760"
                     /pseudo=""
     CDS             <460..642
                     /locus_tag="FZC46_RS25760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001479923.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            843..1079
                     /locus_tag="FZC46_RS25555"
                     /old_locus_tag="FZC46_25860"
     CDS             843..1079
                     /locus_tag="FZC46_RS25555"
                     /old_locus_tag="FZC46_25860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012772745.1"
                     /translation="MARLTNLTPAEKKFLDDAVAAAERASGKKLNQPNRHIVLNRARAQ
                     IELQRYADRQRALREDERQQSDFAWSRPRAPRR"
                     /name="hypothetical protein"
     gene            complement(1293..1772)
                     /locus_tag="FZC46_RS25560"
                     /old_locus_tag="FZC46_25865"
     CDS             complement(1293..1772)
                     /locus_tag="FZC46_RS25560"
                     /old_locus_tag="FZC46_25865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017896843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_230199852.1"
                     /translation="MRKKLSLCVPLFFLAGCAQQKQQMPDANYEKFAKVEVASDACLKA
                     NFITAQEAGQAHSNISLFLSSWAYDYVRYSNILAQGHEELKKIKITQEGCNLLRAKIYQ
                     DTIEAQRYQKQMEMAAQQKAIADQQALQSIQNMQNTLPKTTYCNQIGTQTICNSY"
                     /name="hypothetical protein"
     gene            complement(1863..2294)
                     /locus_tag="FZC46_RS25565"
                     /old_locus_tag="FZC46_25870"
     CDS             complement(1863..2294)
                     /locus_tag="FZC46_RS25565"
                     /old_locus_tag="FZC46_25870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="WP_053274236.1"
                     /translation="MNIPTHLKHKPIIAVENYDRIDGPYADDTDAMGLSVGIAQWNTPG
                     YTELSAKVWRNTGEKWSRQSEELPLHRVIDLATLICIAMDYSKHGRLSSSDDFPVSRSG
                     GNPELSRHIDLMKNELKENHEYLDESLKRLAAELKRLGY"
                     /name="DUF6530 family protein"
     Typing          506..678
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="94.828"
                     /coverage="100.0"
                     /evalue="9.34e-71"
     Typing          complement(17..581)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.474"
                     /coverage="93.0"
                     /evalue="0.0"
ORIGIN
        1 caagaactcc agataaatct ctcgcacatc tcagttacca gtggctgctg ccagtggcgc
       61 tttgtcgtgt ctttccgggt tggactcaag ttgatagtta ccggataagg cgcagcggtc
      121 ggactgaacg gggggttcgt gcatacagtc cagcttggag cgaactgcct acccggaact
      181 gagtgtcagg cgtggaatga gacaaacgcg gccataacag cggaatgaca ccggtaaacc
      241 gaaaggcagg aacaggagag cgcacgaggg agccaccagg gggaaacgcc tggtatcttt
      301 aagtcctgtc gggtttcgcc accactgatt tgagcgtcag atttcgtgat gcttgtcagg
      361 ggggcggagc ctatggaaaa acggcaacgc cgtggcttta tcgcttccct gttaagtttc
      421 ttcctggcat cttccaggaa atttccgccc cattcgtaag cccgcaccgc tcgccgcagc
      481 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aatatatcct gtctcatata
      541 ttctgcttac gctccgctgc tgcatttttc tcctgccaca tgaagcactt cactgattcc
      601 cgcacccgtg ccaacataat aagccagtat acactccgct agcgctacgt gactggttca
      661 gggctgcgcc ccgaaaccgg ctaaaagcta atgacgcgcc tgcggcttgt ccaacgcctc
      721 acggcaccgg attaaaatcc gctcccgttc accgtttctg actgcctttc accgtcgctg
      781 gcgcttcatc gtttctactg tgtctggcag actgtgctga tttattctga ctggagaacg
      841 ttatggccag actgacaaac ttgacgcctg cagagaaaaa atttcttgat gatgctgttg
      901 ccgcagctga aagagcgtca ggtaaaaagc taaatcagcc taaccggcac atcgtcctga
      961 acagagcgcg tgcgcaaatt gagttgcagc gttacgccga ccgccagcgt gcattacgtg
     1021 aggatgagcg gcagcagtcc gattttgcct ggtcacgtcc gcgcgcgccg cgccgctgat
     1081 tttggctttt ggcggtttag tggtgctgac gctgcgaaat cacctgccat tcacctgcgt
     1141 gcatagccat gcagtcgcgc agaaatcatc aggggcgttt ttccgggata ctggtgagga
     1201 aaggtgctgg aaacggccct gatgcagcag gattgcgctg agggcgcctg cgggggtgcg
     1261 ggcaagagat gttatggtaa atcaatttgt gatcagtagc tgttacagat tgtctgtgta
     1321 ccaatctggt tacagtaagt tgttttaggt aaggtgtttt gcatattttg aattgattgt
     1381 aacgcctgct gatctgctat ggccttttgt tgtgctgcca tctccatctg tttttgatat
     1441 ctctgtgcct cgatggtgtc ttgatagatt tttgcgcgca aaaggttgca gccttcctgt
     1501 gttatcttta ttttttttaa ttcttcgtgt ccttgcgcca agatatttga gtaacgaacg
     1561 tagtcatacg cccatgaact taagaacaag gatatgttgg aatgcgcctg acctgcttcc
     1621 tgtgctgtaa tgaagttggc ttttaagcag gcatcagagg ccacctcgac ctttgcgaac
     1681 ttttcgtaat tagcatctgg catttgttgt ttttgttggg cgcagccagc aagaaaaaac
     1741 aacggaacac aaagtgataa cttttttctc attgagtgtt ttatcctttt tgacaagaaa
     1801 aacctaatgc ccccgtggtg gttatataaa aaccacgggg agcgtagcga ccttcgccct
     1861 acttaataac ctaaacgttt tagttctgct gctaaacgct taagactctc atctaaatac
     1921 tcgtgattct cttttagttc gtttttcatc aaatcaatgt gtcgagataa ctcaggatta
     1981 cctcctgaac gactgaccgg gaagtcgtcg ctggaagata agcgaccatg cttactgtaa
     2041 tccatagcta tgcagataag cgttgcgagg tctatgacac ggtgaagagg caactcttca
     2101 gattgccgtg accacttttc tccggtgttg cgccagactt ttgctgatag ctctgtatat
     2161 ccgggggtgt tccattgagc aattccaacg gaaagcccca tggcatctgt gtcatcagcg
     2221 taagggccgt caattcgatc atagttttct acagcgataa tcggtttgtg tttcagatga
     2281 gtagggatat tcatatcatt ctctgctgtg tgaagttgat ttattagtta ataaaacaac
     2341 aaaataataa aatttactaa ttagtaaatg taacattttg tgaattactg aatttggagt
     2401 tgttggagta gatcttcttg agatcattta tggctgagcg taatctcttg ctctgaaaac
     2461 gaaaaaacca cctggggagg tggtttgatc gaaggttagg tcagttgggg aactgcttaa
     2521 ccaggtaact gtcttgtgcg agacgagtca ccaaaactgt ccttccagtg tagccgtact
     2581 agggctccca ctt
//
