LOCUS       NZ_CP043298             4061 bp    DNA     circular CON 24-MAY-2024
DEFINITION  Escherichia coli strain WCHEC040047 plasmid p2_040047, complete
            sequence.
ACCESSION   NZ_CP043298
VERSION     NZ_CP043298.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN08932753
            Assembly: GCF_022559045.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Tang,G., Long,H., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Escherichia coli strain WCHEC040047
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4061)
  AUTHORS   Tang,G., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-AUG-2019) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022559045.1-RS_2024_05_24
            Annotation Date                   :: 05/24/2024 04:28:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,121
            CDSs (total)                      :: 5,001
            Genes (coding)                    :: 4,817
            CDSs (with protein)               :: 4,817
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 66 of 184
            Pseudo Genes (incomplete)         :: 127 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 36 of 184
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP043298.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="WCHEC040047"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p2_040047"
                     /geo_loc_name="China: Chengdu, Sichuan"
                     /collection_date="2017"
     gene            3..704
                     /locus_tag="FYK54_RS25510"
                     /old_locus_tag="FYK54_25855"
     CDS             3..704
                     /locus_tag="FYK54_RS25510"
                     /old_locus_tag="FYK54_25855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281395.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPDE
                     QKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     FKGEAKVDITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(950..3097)
                     /gene="mobQ"
                     /locus_tag="FYK54_RS25515"
                     /old_locus_tag="FYK54_25860"
     CDS             complement(950..3097)
                     /gene="mobQ"
                     /locus_tag="FYK54_RS25515"
                     /old_locus_tag="FYK54_25860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_087529035.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAIGPAGFSGKVRDWNDRTHAET
                     WRASWADHANRALANASYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNGGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     SAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQWQQWGESGLAALRAQL
                     LKREEELRRKEQEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQRPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSPAARKVDFMQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSLGGVIRLNGTLYPAFAVPSADNRHLVISALTDKGLRYA
                     GYGVAVNHDADSHISPAPELMQFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRTWDEW
                     RREEDARTHATERRHDRGWSQ"
                     /name="mobQ"
     gene            3276..3530
                     /gene="mobC"
                     /locus_tag="FYK54_RS25520"
                     /old_locus_tag="FYK54_25865"
     CDS             3276..3530
                     /gene="mobC"
                     /locus_tag="FYK54_RS25520"
                     /old_locus_tag="FYK54_25865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091746.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     Typing          complement(2740..3098)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3099..3275
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          4..704
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.858"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(954..3184)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.257"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaatgtcaga tctgatagct tacaaatcaa acgcccttat cgaggcaagc tacaagctca
       61 ctttgcagga acagcgcttc ctgctggtct gtatcagccg cctaaaatcc ggagaagatt
      121 ccccttcgcc ggatgaacaa aaaaccatga ccatcacggc tgcagagttt catgatgcct
      181 ttcctgacat gggacggcag cacgctgaag ctaggctcaa ggaagcgata gatcgcctct
      241 gggacaggtc agtaatcatc aagaacgagg aaaaacgaga agagttccgc tgggtgcagt
      301 accgggcgca gtatttcaaa ggtgaggcga aagtagatat caccttctcc gatgccatca
      361 tgccgtatct gacacagctc aaggggcagt ttacgcgggt agtcgtgaag aacgtctcca
      421 gcctttccag cacctacagc atccgcattt acgagctgct ccagcagttc cgcagcaccg
      481 gtgacagaac gatagctatc gatgatttcc gaaccatgct tgccgttgaa gataaatatc
      541 aacaattcaa agacttgaat aagatgataa tcaagccagc catcgccgaa ctgaacgaaa
      601 aaagcgatct ggttgtaact gttgagacga tcaaaaaggg gcgcacggtt gtagccctgc
      661 attttcgctt caaggaagac aaacaaatca agatgacgat ctaaaatcga ttttaaggca
      721 cctaaaacgc tccctgctgc gttttttttg tgttagtagt gcgaaggtac tattgaagtg
      781 aaaaattgcg tcaaaagcac tctaatgcag cgaaaaaaga acccccattt tttgggggcg
      841 gaaaaacaaa actatgcttg cattatgtgt gtgcttcttg cattcatccc tgtcgatgaa
      901 catcgaagaa cataacacga tgttaaacaa aacaaaatat tgttcacatt cactgtgacc
      961 aaccgcgatc atgtctcctc tcggtggcgt gtgttcgtgc atcttcctcg cgccgccatt
     1021 cgtcccaggt acgattaaaa cccagcgagc ggaacagatg atccggctgt ttttctggtg
     1081 tgttgacggc agcaaaaagc ggtgcctcac gggttttcag atgaaactgc atcagttctg
     1141 gtgcaggtga tatgtggctg tctgcatcgt gatttacggc aacaccatac cctgcatacc
     1201 gtaacccctt atcggtcagc gcactgatga ccagatgcct gttatcggct gagggaacgg
     1261 caaacgcggg atacagcgta ccattaagcc ggataacacc gcccagagag tcatccacgc
     1321 ccatgaccag caaaagctga agctggcggg tttcccggtc gacagaactc atgaccggca
     1381 tcagcgtcgg tggcagacgt ccctgcataa aatccacctt ccttgccgct ggtgatggca
     1441 tggctggctg gcggtcttct ccaagacaga aagactccgg ccatgtcttc ggctggcgga
     1501 gattgcgggt atgttgcggc catgtctggc aaacctgcgt cagcagttgc tcaaaggttg
     1561 cagaggaata aacacgttgc agtggcggac gttggccgat ttcgcttcgg tcacagatat
     1621 aaccactcct gtcacgcatt aaatcaacac tctcaaagat tctggtgcgg tatttctcca
     1681 gctcgttccc ccagagcgta tattctccgt tccggttgtg aagcagaagg taacaggtca
     1741 tttccccgtt accaggcatg ggagcctcgc cataccccgt aacgactgct ctcagcaccc
     1801 ctgaatccat aacggcattg tcatttttcc tgagctgctc cgcgcgacta cgttcagtat
     1861 cttcctgttc cttccggcgc aattcctctt cacgtttaag caactgggct ctgagagctg
     1921 caaggccgga ttccccccac tgctgccact gtttttcaaa tgccatctga ctcgcgggaa
     1981 tgtcctgttt ctccaccttc cggtaatgct gtcgtaattt ttccatttgc tggctcagag
     2041 actggatttt tccctgctca ctacggtatt ccggtgccgc ccattgcatc aggggaatac
     2101 gggaaaaacc ggacgaaaac ggcgatgatt ttttcaggct ggcagcatgg ctcatcgctt
     2161 cctttcgttc ggtgttcagc tcttcccagc gtctgcgcgt cgcctgcata tctgccatca
     2221 tcgacatggc attactggtg agtttccatg ccgagttact gtccttcggc aacgcttcga
     2281 taatgtcacg tagtgtgtac gaagttgcgt tgtcaggaag cccgatattc agaaggtcag
     2341 ccacacggcg ggcaggctct gtcagccctc cgttcctgag ttcggtgcgg gaaatctgta
     2401 tttccaggtt aagcctgtta atcagacgat tctgttcgcc gcgttctgtt tcgataccgc
     2461 gtgtttccat cgcacaggca gactttccga ggtgaatacc aggcgttttc tcaagcccct
     2521 gtcgctcgta tgaacgatgg tctatctctt cctgataact ggcgttcgcc agtgccctgt
     2581 ttgcatggtc agcccatgat gcccgccacg tctcggcatg tgtccggtcg ttccagtccc
     2641 tgacctttcc gctgaaccca gcaggtccga tagccctcgt ggtcagcatg atgtgcgcat
     2701 ggggatttgt tttatccatg tgatggaacg ccacatcagc aatcatgccc tgactgacaa
     2761 agttttctct gacaaaggcg agaacggttt cccgtgcggc atcctgcggc agttcgtggg
     2821 ggatggcgag ttcaatttcc cgcgcaagtt gtgagttctt ccggcgttcg gccttctcaa
     2881 cggcattcca gagttcggca cggttcgtac accaggcagg ggcggtggcg ggggtcagaa
     2941 tcacagcctc agccacaccg cttttgcggg tgtagtcgtg agtcagtccg gtacgctcat
     3001 caagcatcag tgaaccggaa cgataggcag cggaagctac ggcgctgtag ccgctgcttc
     3061 tggagataat tttcatgctg agatgataaa tcgccatttc tgccgtaatg cctttttgta
     3121 gttttctttc agccccgcag ggcgcacaca atcgaaacgg agtggagatt gtataagtgc
     3181 gcacttcgtg atttgccgtg cattatgcca gctcagtttt taaactacca gtgacgcgct
     3241 aaaatgcgat ttttcatcgc gcacggagtt acatcatgag cagaacgctc gaacagaaga
     3301 ttgctgatgc tgaggcgaga ttacagcgcc tgaaggcgaa gagccggagc ctggacacag
     3361 cgcagaaggt tgttgtgggt gctgcattgc tggcaaaggt cagaaagccg gaggaggtgc
     3421 agttgcgtgc ctggttactc cagttcctga aggcagaggt gacacgacag gctgatgtga
     3481 cgcgcatact gccgctgatt aacgagctgg aggcgctgcc agaacagtga ttgtatcgtc
     3541 cgctcgccgc agccccagcg accgagcgtt agcgagcgag tgggcgagga agcggaagtg
     3601 aatcagtcgc caggttgagc acatacgcac ggtttttcgg caggtgatat gcgctgaacc
     3661 cgcatgaata ctgggtttcc acagggttgg agggggtgta ttcgaaactt gcaattctgc
     3721 tttattgcaa cgcattgttt ttattggttt tttgttttca gcaccccaag ttatccacag
     3781 gctcgccgtt aagtttttaa gatcttattt ttagatcttt ttttttaaaa cctttttagg
     3841 tcttgtaaca ccatgatttt aaagatgttc tggcaagata agtggagctt ttgggggtga
     3901 aaggtggagc ttttgggggt gaaaggtgga gcttttgggg gtgaaaggtg gagcttttgg
     3961 gggtgtaatc cacagaaaaa gggggttgca aaaaaaaaac ttccacccat atgataagtg
     4021 gcgtacagta aaaaaacgcc accaatagca acgagcaagc g
//
