LOCUS       NZ_CP043292             2255 bp    DNA     circular CON 24-MAY-2024
DEFINITION  Escherichia coli strain WCHEC025985 plasmid p5_025985, complete
            sequence.
ACCESSION   NZ_CP043292
VERSION     NZ_CP043292.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12158048
            Assembly: GCF_022559025.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Long,H., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Escherichia coli strain WCHEC025985
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-AUG-2019) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022559025.1-RS_2024_05_24
            Annotation Date                   :: 05/24/2024 04:20:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,230
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,788
            CDSs (with protein)               :: 4,788
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 97 of 320
            Pseudo Genes (incomplete)         :: 228 of 320
            Pseudo Genes (internal stop)      :: 47 of 320
            Pseudo Genes (multiple problems)  :: 48 of 320
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP043292.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="WCHEC025985"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p5_025985"
                     /geo_loc_name="China: Chengdu, Sichuan"
                     /collection_date="2018-05"
     gene            complement(join(2200..2255,1..460))
                     /locus_tag="FYK42_RS25995"
                     /old_locus_tag="FYK42_26470"
     CDS             complement(join(2200..2255,1..460))
                     /locus_tag="FYK42_RS25995"
                     /old_locus_tag="FYK42_26470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097059.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_000097059.1"
                     /translation="MSSLSLQRYETNPFLELMTIPMKGKRIQVSRLGRDDNVLVNQNTG
                     EKFGTHVTTFKRVDSEQFVKLFTANIGLTFDLSSAGIKAFNVLLWAVQKQAFAKDQVLL
                     DSRALDDFLKSYDSLKLSYATLKRGINELEKAQIIAKTMRKGFYFINPNFVFNGDRIAF
                     TTLIERDC"
                     /name="replication/maintenance protein RepL"
     gene            complement(1235..1738)
                     /gene="ddp1"
                     /locus_tag="FYK42_RS26000"
                     /old_locus_tag="FYK42_26485"
     CDS             complement(1235..1738)
                     /gene="ddp1"
                     /locus_tag="FYK42_RS26000"
                     /old_locus_tag="FYK42_26485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA distortion polypeptide 1"
                     /protein_id="WP_000809479.1"
                     /translation="MKQIKFRLTDEEYNVAHINAISNGVNSVNAFAKRKVLDISATPSK
                     VIVSNEPSVPVKTCLYPHELELIKRNAALHGMSVSREIAIRLRKSVLKSEVCLYPEEVQ
                     VLRKLSTTINRIGRNIHFIISGDRFCTVNDPDFRNEIRELTALCETVDKTIEQLVFSVN
                     NRFG"
                     /name="ddp1"
     gene            complement(1841..2125)
                     /locus_tag="FYK42_RS26005"
                     /old_locus_tag="FYK42_26490"
     CDS             complement(1841..2125)
                     /locus_tag="FYK42_RS26005"
                     /old_locus_tag="FYK42_26490"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000160398.1"
                     /translation="MTKHDAIRISQLHQIFYDDEGLIDSEKNVTILYSIGFTIDEIAYF
                     GNTTPGTVQGQLFNARAKLSCASASSLRPMILLRLLSLIIFEQDRLNRL"
                     /name="hypothetical protein"
     Typing          730..981
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.466"
                     /coverage="97.0"
                     /evalue="3.95e-106"
ORIGIN
        1 agttaggatt tatgaaataa aatccttttc gcattgtctt tgctataatc tgagcttttt
       61 ctaactcgtt gatccctcgc tttaacgtag cgtaacttag ttttaagcta tcatagcttt
      121 tcaaaaagtc atcaagagct ctggaatcta aaagaacctg atcttttgca aaagcctgtt
      181 tttgaacagc ccataacagg acattaaaag ctttaattcc tgccgatgat aaatcaaatg
      241 ttagcccaat attcgcagta aacaatttaa caaattgctc actatccaca cgcttaaaag
      301 tggttacatg cgtcccaaac ttttctcccg tgttttgatt aaccaaaacg ttgtcatcac
      361 gtccaagacg agatacttgt atccgtttcc ccttcatcgg gattgtcata agctctaaaa
      421 atgggttcgt ttcgtatctc tgcaagctta gtgagctcat tattgcatcc ttttttgggc
      481 tttggagatt cacaataaat cctcataagc tcaaaatcaa tgattttttg agcctgcaag
      541 gtaccggatc tgagcttaca aggtacagat cctgatcacc actttctagg attcatgcgg
      601 gtttcaagaa attcccttct tagttcttaa ctacgagcgt aagtgctcaa tatggcttct
      661 ggttctcttc cgcttcctcg cccactcgct cactacgttc ggtcgctggg gctgcggcga
      721 gcggtgtcac ctctcgcatg ttgtcggtga tcccgttgtt attctttcgg ggatgagtcg
      781 tcagatagcc cctgaaacgc gctcagaagc tctcagacac acgagacggg gtttaaatgc
      841 ccgtcagggc atggaaggcg cgaaaaacgc ttacagaggg tttcaggagg tcaggcgcgc
      901 cgatacccct aaccaggggc tttcaggtcg ccaggaaccg cgtcagcccc ctgcaaagcc
      961 catttgcacg gaacaggcag ccacgcggtt ggtaggtggt cgtagaccgt cggtgttgcc
     1021 gcgatggggg tctggggttc tccccagtcg gtttccggtt gcgctcaagg cgcaatcgga
     1081 accgtggcgg cttcggtcgt tccgaagaac ggggaataaa aatcttcgtg gtttaacccc
     1141 gatgcaggat agggcaaacg ccgcaaaatg acgtctctga cgcaattctg caacgtttgc
     1201 ccatgaccta cagtcggttc ttgataatac gtttttaccc aaaacgatta ttaacactaa
     1261 aaactaattg ctctattgtt ttatcgactg tttcgcataa tgctgtaagt tcccttatct
     1321 catttctgaa atcaggatcg ttaactgtgc aaaaacgatc gccactaatt ataaaatgaa
     1381 tatttcgacc aattctatta atcgttgttg aaagctttct gagtacctgc acttcttcgg
     1441 gataaagaca aacttcgctt ttcaacactg atttacgtaa gcgtattgct atttctcggc
     1501 taacgctcat cccgtgtaat gcagcattac gctttattaa ctccagttca tgtgggtata
     1561 aacatgtttt tactggtaca gatggttcat tcgatacgat aacctttgaa ggtgttgcgc
     1621 taatatctaa aaccttcctc tttgcaaaag catttacact attaacgcca tttgaaatag
     1681 cattaatatg agcaacgttg tattcctcgt cggtcaatct gaatttaatt tgcttcatag
     1741 aacctcctaa gagcctgcgg caggcacagg caaaaacccc gtttttggac aatcatggaa
     1801 aatggtaccc atttgaccat gattgtctat cctgcattga ttacagccta tttaagcggt
     1861 cttgctcaaa gattatgagt gaaagcaatc tcaacaaaat catcggtctt aaagatgaag
     1921 ctgaagcaca acttaattta gctcttgcat taaataactg tccctgaact gttcccggtg
     1981 ttgtattccc aaaataagct atttcatcaa tagtaaatcc tatgctgtat aaaatggtca
     2041 cgttcttttc tgaatcaatt aatccctcgt catcgtaaaa gatttgatga agttgagaaa
     2101 tgcgtatagc atcatgctta gtcataataa cctccctaat aggttgttgt gattagcagt
     2161 cgaacaaaag tggagttttg ttcgactgcg acgattatat taacaatccc tctcgatcag
     2221 agttgtaaac gctattctat cgccattaaa tacaa
//
