LOCUS       NZ_CP043283             3684 bp    DNA     circular CON 24-MAY-2024
DEFINITION  Escherichia coli strain WCHEC025157 plasmid p2_025157, complete
            sequence.
ACCESSION   NZ_CP043283
VERSION     NZ_CP043283.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12623215
            Assembly: GCF_022559005.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Long,H., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Escherichia coli strain WCHEC025157
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-AUG-2019) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022559005.1-RS_2024_05_24
            Annotation Date                   :: 05/24/2024 04:26:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,936
            CDSs (total)                      :: 4,816
            Genes (coding)                    :: 4,573
            CDSs (with protein)               :: 4,573
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 243
            CDSs (without protein)            :: 243
            Pseudo Genes (ambiguous residues) :: 0 of 243
            Pseudo Genes (frameshifted)       :: 86 of 243
            Pseudo Genes (incomplete)         :: 160 of 243
            Pseudo Genes (internal stop)      :: 49 of 243
            Pseudo Genes (multiple problems)  :: 46 of 243
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP043283.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="WCHEC025157"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p2_025157"
                     /geo_loc_name="China: Chengdu, Sichuan"
                     /collection_date="2017-12"
     gene            complement(join(3495..3684,1..233))
                     /locus_tag="FYK41_RS24500"
                     /old_locus_tag="FYK41_24860"
     CDS             complement(join(3495..3684,1..233))
                     /locus_tag="FYK41_RS24500"
                     /old_locus_tag="FYK41_24860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_039023181.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVLKHGKTIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAGSTGVNSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPDSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(233..424)
                     /locus_tag="FYK41_RS24505"
                     /old_locus_tag="FYK41_24865"
     CDS             complement(233..424)
                     /locus_tag="FYK41_RS24505"
                     /old_locus_tag="FYK41_24865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_039023180.1"
                     /translation="MSKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            829..975
                     /locus_tag="FYK41_RS24510"
     CDS             829..975
                     /locus_tag="FYK41_RS24510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_073545459.1"
                     /translation="MHEPPVQSDCCTLSGNYHLESNPVRHAQAPLAAATGKKVYKRLLI
                     QSS"
                     /name="replication initiation protein"
     gene            complement(1433..1714)
                     /locus_tag="FYK41_RS24515"
                     /old_locus_tag="FYK41_24875"
     CDS             complement(1433..1714)
                     /locus_tag="FYK41_RS24515"
                     /old_locus_tag="FYK41_24875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_032440460.1"
                     /translation="MIKNIKHKGLRSFAEEGSTAGIQPSMAKRLRMILTTLSTATCIQD
                     VVDVRALGCHPLKGERKGEYSVEVNGNWRVVFKVEEPNVLDVDLEDYH"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            2051..2344
                     /locus_tag="FYK41_RS24520"
                     /old_locus_tag="FYK41_24880"
     CDS             2051..2344
                     /locus_tag="FYK41_RS24520"
                     /old_locus_tag="FYK41_24880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609279.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_023317864.1"
                     /translation="MINLYDPVHPGEFIREMFMEPFEIPAAELADKLDVSQSTLSRLLN
                     GKTDLSYEMALRLSKVVGRSAESWVNMQTQFSLSKSRKSLAAELEHLHPMFP"
                     /name="HigA family addiction module antitoxin"
     gene            2408..2710
                     /locus_tag="FYK41_RS24525"
                     /old_locus_tag="FYK41_24885"
     CDS             2408..2710
                     /locus_tag="FYK41_RS24525"
                     /old_locus_tag="FYK41_24885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006326557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3892 domain-containing protein"
                     /protein_id="WP_039023182.1"
                     /translation="MVDFYISAIKMDSAGQHIEFVKVHKINRDGSWNTAGSISSRKFIS
                     ELISMGKASFMTVVKNSSTGKWNLGAKVEAMDDGYITTDPNKTKLDNLGNLPKFV"
                     /name="DUF3892 domain-containing protein"
     Typing          566..938
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.467"
                     /coverage="91.0"
                     /evalue="1.28e-167"
ORIGIN
        1 ctgttaacac ctgtagatcc tgcgtcactt tttacatcat attttacagt atcgatgaca
       61 ccatcaagag aaccaaaaac tctaataagc tctgaagtat caataacttt aatggtcttc
      121 ccgtgcttta aaacagaggt gtaacttaac ttcccactgt taatcatctt gtaaattgtg
      181 gggcgactaa ccttaacaag ccgtgccgct tcactaatag acactctggc catcagttac
      241 catcctgtaa ttcatccagt ctcgaggaca gtgtacggaa aagatgctcc gcatcatcgt
      301 ggagtttctc acaaaggtcg gcctctgcat caagatcaag ctcgttaagc ttctccagca
      361 ggagcagaga ctgattctgg atgaatttag ccatgttcag cgctgttttc tgttgtttcg
      421 acataagagg cctcagagcg tcagtgctca gtgtaggtat gttctctttt ccgcttcctc
      481 gctcactcag tcgctacgct cctgccgttc ggctgcggcg agcggtcgca atacacacat
      541 aacgacatag gacagacagc acggagaggg agaaggccgc acaccggccg tttttccata
      601 ggctccgccc ccctgacaag catcacgaaa tctgacgctc aaatcagtgg tggcgaaccc
      661 cgacaggact ataaagatac caggcgtttc cccctgatgg ctccctcgtg cgctctcctg
      721 ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcgttta tctcattcca
      781 cgccagacac tcagttccgg gtaggcagtt cgctccaagc tggactgtat gcacgaaccc
      841 cccgttcagt ccgactgctg caccttatcc ggtaactatc atcttgagtc caacccggta
      901 agacacgcac aagcgccact ggcagcagcc actggtaaga aggtgtacaa gagattactg
      961 atacagagtt cttgaagtct cagcccaact acggctaaac tgaaaggaca gaatttggtt
     1021 tcagcggtct tgtacaccct gttaccacgg ttcagaagtt ccccaactta ctgaaccctc
     1081 gaaaaaaccg cctccccagg cggttttttc gtctcggcga gtaagatacg ctttatcatt
     1141 caaagcagtc aaggaagaac caagacagct cactcaccag caggctcctt tttattggta
     1201 gacacatgct tctggacagt atcagcccct gcacaaagta cagcagctaa caagaaccat
     1261 ccccagccat caataccacg aaaagctagg actccagcaa tagttacgct aatcaacggg
     1321 aacaataaaa ccataaagga tctcatactt tctccagagg ttactttttt aataaagtcc
     1381 ctaatccatc cgtactttca cttggatgga ttagcaactt acttattgat aactaatgat
     1441 aatcttcaag atcaacgtct aagacgtttg gttcctcaac cttgaaaact acccgccagt
     1501 tcccattaac ttcaacggaa tactcgcctt tacgctcccc ctttaggggg tggcacccta
     1561 acgccctaac gtcaacaacg tcttgaatgc aagttgctgt gcttagcgta gtcaaaatca
     1621 tgcgaagtct tttcgccata ctgggctgaa taccggcggt gctaccttct tcagcaaagc
     1681 tcctaagtcc tttatgtttt atgtttttta tcatctacaa tccccatatt tttttcgtct
     1741 atacctgtat ctatatttta aaatcatttt aattagttcc attttgcccc cttgctttat
     1801 taaaacaatt gacgtaagtt tgttcttgag gtcaatcacc tcatttgact tcaacgaatc
     1861 ctaagggtaa aatactgcgc cttgaaaagg ccataaaagc ccatacatac gcagtagctt
     1921 tgactccaac gagtatccta aagctgactg ctttttttgt caatatcatg aacttgcaca
     1981 ttgtgcaatg cgcatactga aaggcagtaa tttttttgta taatcaaata caaaacaaac
     2041 agaggaaggc atgataaatc tttacgatcc tgttcatcct ggtgagttta tcagagaaat
     2101 gttcatggaa ccgttcgaaa tacctgccgc agagttagcc gataaacttg atgtatccca
     2161 gtctacctta agtagactgt taaatgggaa aacagactta agctatgaaa tggcacttag
     2221 attgtcgaag gtcgtaggcc gctctgcaga gtcgtgggta aacatgcaga cccaattcag
     2281 cctgtcaaaa agtcgcaaat ctttagcggc agagctggaa cacttgcatc caatgtttcc
     2341 atgaaaaaag cccgcagaac gcgggctttt gttacgtaaa acacccagca gccatcagga
     2401 ggcaaccatg gtagatttct atatctcagc aatcaaaatg gacagtgctg gtcagcacat
     2461 cgaattcgta aaagttcata aaataaaccg agatggctca tggaacactg ccggctcaat
     2521 cagctctcgc aaattcatca gcgagctgat cagcatggga aaagccagct ttatgacagt
     2581 tgtaaagaac agctctactg gtaagtggaa cctaggagcc aaagtggaag cgatggacga
     2641 tggctacata accacagatc caaacaaaac taaactcgat aacttgggaa atctgccgaa
     2701 atttgtctga tatgcaataa catccactaa gttccagcag aatcccctcc ctaatgattt
     2761 aacataaagt cataattgta cgcaccgcca gaacggcctc agggcgtttc tgacaggaaa
     2821 ccggtaaaac aggcaacaaa ccacccgaaa acccgtcaga aacgcgcagg ctctgttttt
     2881 acggcatgca tgagtatgca cgcaagtgca tagcgcgaaa attcacgcca ttcagccagg
     2941 gtaagtcctg tttccggcgt tcttcccatc cgaaaacggt gaacggggac gcaatttatt
     3001 gcgtggccgt gaagcgttgc cggcgcagcc ggaaaaccgg ttttcagcac gttcaggaat
     3061 cgtgttcgtc gacggccggc aatcacgtca tggcaagtcg ccggacgact caccacacga
     3121 ttcaccggcg actcttcact agagtcgtac ccagagtcgc cgagtctaaa aaacaaccaa
     3181 acggggagtc gggtctagag tcgggtcgtt attaactcat tgtttttata gctctgagtc
     3241 gttaccgact ctatgacgac tcgccgggga ctctatgacg agtcgtcata gagtcggatc
     3301 tatgtaaata aaatttaaaa accgaaactt caggaaagta agctactgat attaaataca
     3361 aatctgcaaa attttaattt tgcgtagtgt gtgggtattt ttagtcgtga ggagcgttca
     3421 gagatctggc gatttttggg aaggttcagg aactccccgg gatggccgga gaggaaaacg
     3481 gaagcatgac ggagtcagga ttttttccag aatttccacc acggggaatc gggtggtgaa
     3541 gaaggttcgt gcttgtgctc cagaagctgc atagcctgac gaagggaatc aatatgctca
     3601 tctcgagcct taacagcccc tttaagacca tcgttttcag cttctagtaa agcaattcta
     3661 tgttgtaaat catgtaaacc aacg
//
