LOCUS       NZ_CP043221             1552 bp    DNA     circular CON 20-JUN-2024
DEFINITION  Escherichia coli O80:H26 strain EC-107 plasmid pET6.4-ColRNAI,
            complete sequence.
ACCESSION   NZ_CP043221
VERSION     NZ_CP043221.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12603254
            Assembly: GCF_008124005.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O80:H26
  ORGANISM  Escherichia coli O80:H26
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Laskey,A., Ottenbrite,M., Devenish,J., Kang,M., Savic,M., Nadin
            Davis,S., Chmara,J., Lin,M., Robertson,J., Bessonov,K., Nash,J.,
            Scott,A., Topp,E., Gurnik,S., Liu,K. and Guan,J.
  TITLE     Inflammatory infection and transmission of beta-lactam resistance in
            a mouse model of ampicillin-induced microbiota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Laskey,A., Ottenbrite,M., Devenish,J., Kang,M., Savic,M., Nadin
            Davis,S., Chmara,J., Lin,M., Robertson,J., Bessonov,K., Nash,J.,
            Scott,A., Topp,E., Gurnik,S., Liu,K. and Guan,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-AUG-2019) Public Health Agency of Canada, National
            Microbiology Laboratory at Guelph, 110 Stone Road West, Guelph,
            Ontario N1G3W4, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3.1
            Assembly Name                     :: ET6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 379x
            Sequencing Technology             :: Illumina MiSeq and PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_008124005.1-RS_2024_06_20
            Annotation Date                   :: 06/20/2024 03:39:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,345
            CDSs (total)                      :: 5,213
            Genes (coding)                    :: 4,953
            CDSs (with protein)               :: 4,953
            Genes (RNA)                       :: 132
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 79 of 260
            Pseudo Genes (incomplete)         :: 180 of 260
            Pseudo Genes (internal stop)      :: 40 of 260
            Pseudo Genes (multiple problems)  :: 37 of 260
            Pseudo Genes (short protein)      :: 1 of 260
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP043221.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Escherichia coli O80:H26"
                     /mol_type="genomic DNA"
                     /strain="EC-107"
                     /serotype="O80:H26"
                     /isolation_source="chicken"
                     /db_xref="taxon:2605620"
                     /plasmid="pET6.4-ColRNAI"
                     /collection_date="2016-12-19"
                     /collected_by="Public Health Agency of Canada"
     gene            complement(667..1143)
                     /locus_tag="FYA02_RS26970"
                     /old_locus_tag="FYA02_26970"
     CDS             complement(667..1143)
                     /locus_tag="FYA02_RS26970"
                     /old_locus_tag="FYA02_26970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000469298.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVRE
                     LLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKNAL
                     VRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
                     /name="helix-turn-helix domain-containing protein"
     Typing          1223..1331
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.95e-50"
     Typing          177..437
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.13"
                     /coverage="100.0"
                     /evalue="1.84e-104"
ORIGIN
        1 atgtgcaaat gtaataccgt cacccgcgac gatattccgt ttgccatctg gtcagggctt
       61 cgccccgaca ccccgtatca gtgcgtacgt gctcaatgtg gtttccggtt ctcttccgct
      121 tcctcgccca ctcgctcact aacgttcggt cgctggggct gcggcgagcg gtgccacctc
      181 tcgcatgttg tcggtgattc cgctgttgtt ctttcgggga tgagtcgtca gatagccctt
      241 caaacgcgct cagaagctct cagacgcacg aggcggggtt taaatgcccg tcagggcatg
      301 gaaggcgcga aaaacgctta cagagcgttt caggaggtca ggcgcgccga tacccctagc
      361 caggggcttt caggtcgcca ggaaccgcgc cagccctctg caaagcccat ttgcacggta
      421 caggcagtca ctcggttggt aggtggtcgt agaccgtcgg tgttacagcg atgggggtct
      481 ggggttctcc ccagacgatt tccggttgcg ctcaaggcgc tgccggaacc gtggcggctc
      541 ggtcgttctg gagatcgggg agcaaaaaca cgcaagggat tttgaggcgg caaaagagca
      601 atgcaggata gacaatcatg gtcagaacgt taacacgtag cccctgattg tccgcctgcc
      661 gctggctcaa tttgaaaaca tggccatgat tcggcgctga atatcaccgt ctttgttgac
      721 cagtgtttta gtgacaaatg ctgcatggtt tttcgtagtg ttgtatccct tccagacttc
      781 atgtggatcg agggcatacg cacaaacctt accagcaacg gggattctaa ctaatgcatt
      841 ttttgattct aagtaattga gccaacgaat aattgtgctt ctgctaactc caagtttttc
      901 agctaggaat tgctgatccg caacgacagc gccacacgta ggatcgatat gctccgctaa
      961 aaatgagtac agcttcagtg ctgatatgcc ttggctatcc ttcagcagct ctcgaacgcg
     1021 ttcccaacct tttggggata cctgtgtaaa ccttgaattc ttctttgctt cttcgagatc
     1081 ctcggtgttt ttctttgcct catctgaaag ttgtttcagt cgtttttctg aatcaataaa
     1141 catgattttt tccctctaaa aatcccatgc actaacgttg gttcaaatct aagccatcat
     1201 gacacatctg tgcaacacct tgttgcgtgt tgtcgcatcc aggatgcgac aacgttgtgt
     1261 caccctggat gcgacaggta aacaaaaaac acactaaaaa aacaacaact tacaaatcgg
     1321 cgtaaaagat ctattctagg caagggtgtt gcgtgcaacg caacgccctt tgggggtgtc
     1381 ggggcgaagc cctgacccag tcacgtagcg atagcggagt gtatactggc ttactcctct
     1441 gcaagcctcg ccagccgtga tttttgccag atttcgtggt tttcacgttc aacagctcct
     1501 gcacgttttt tgccgtccgt tcaccgttaa cccccgcaga aatcgggaca gg
//
