LOCUS       NZ_CP042891             3638 bp    DNA     circular CON 13-JUN-2023
DEFINITION  Escherichia coli O10:H32 strain NMBU-W12E19 plasmid pNMBU-W12E19_07,
            complete sequence.
ACCESSION   NZ_CP042891
VERSION     NZ_CP042891.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12565117
            Assembly: GCF_013282315.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O10:H32
  ORGANISM  Escherichia coli O10:H32
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Finton,M.D., Meisal,R., Porcellato,D., Brandal,L.T. and
            Lindstedt,B.-A.
  TITLE     Multidrug-resistant environmental bacterial isolates, Norway
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3638)
  AUTHORS   Finton,M.D., Meisal,R., Porcellato,D., Brandal,L.T. and
            Lindstedt,B.-A.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2019) Faculty of Chemistry, Biotechnology and Food
            Science, Norwegian University of Life Sciences, Chr. Magnus
            Falsensv. 1, Aas N-1430, Norway
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 06-AUG-2019
            Assembly Method                   :: Unicycler v. 0.4.7.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 34.78x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/13/2023 04:17:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,556
            CDSs (total)                      :: 5,427
            Genes (coding)                    :: 5,049
            CDSs (with protein)               :: 5,049
            Genes (RNA)                       :: 129
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 126 of 378
            Pseudo Genes (incomplete)         :: 250 of 378
            Pseudo Genes (internal stop)      :: 61 of 378
            Pseudo Genes (multiple problems)  :: 56 of 378
            Pseudo Genes (short protein)      :: 5 of 378
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP042891.1.
            Bacteria and source DNA available from Prof. B-A. Lindstedt, KBM,
            Norwegian University of Life Sciences, P.O.Box 5003, N-1432 Aas,
            Norway.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Escherichia coli O10:H32"
                     /mol_type="genomic DNA"
                     /strain="NMBU-W12E19"
                     /serotype="O10:H32"
                     /isolation_source="surface water"
                     /db_xref="taxon:2603836"
                     /plasmid="pNMBU-W12E19_07"
                     /geo_loc_name="Norway:Aas"
                     /lat_lon="59.667 N 10.769 E"
                     /collection_date="2019-02"
                     /collected_by="Cecilie Fagerland"
                     /genotype="ST1286"
     gene            complement(839..>937)
                     /locus_tag="FVP48_RS27770"
                     /pseudo=""
     CDS             complement(839..>937)
                     /locus_tag="FVP48_RS27770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            <936..1019
                     /locus_tag="FVP48_RS27775"
                     /pseudo=""
     CDS             <936..1019
                     /locus_tag="FVP48_RS27775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463203.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(1016..1333)
                     /gene="sugE"
                     /locus_tag="FVP48_RS02350"
                     /old_locus_tag="FVP48_02660"
     CDS             complement(1016..1333)
                     /gene="sugE"
                     /locus_tag="FVP48_RS02350"
                     /old_locus_tag="FVP48_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463202.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="WP_000118520.1"
                     /translation="MSWIVLLIAGLLEVVWAIGLKYTHGFTRLTPSIITIAAMIVSIAM
                     LSWAMRTLPVGTAYAVWTGIGAVGAAITGILLLGESASPARLLSLGLIVAGIIGLKLST
                     H"
                     /name="sugE"
     gene            complement(<1444..1875)
                     /locus_tag="FVP48_RS02355"
                     /old_locus_tag="FVP48_02665"
                     /pseudo=""
     CDS             complement(<1444..1875)
                     /locus_tag="FVP48_RS02355"
                     /old_locus_tag="FVP48_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(1878..2804)
                     /locus_tag="FVP48_RS02360"
                     /old_locus_tag="FVP48_02670"
     CDS             complement(1878..2804)
                     /locus_tag="FVP48_RS02360"
                     /old_locus_tag="FVP48_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001314449.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTAEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            2980..3375
                     /locus_tag="FVP48_RS02365"
                     /old_locus_tag="FVP48_02675"
     CDS             2980..3375
                     /locus_tag="FVP48_RS02365"
                     /old_locus_tag="FVP48_02675"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_094321956.1"
                     /translation="MRISNIDWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNELQAQENARKQANQKRMEGKTQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELRYQKDNILQKLKAIRLDLETSPDHH"
                     /name="hypothetical protein"
     Typing          complement(1882..2804)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tggtggaagg tcggagggaa cggcaacaac tcacccctcc cagtgaaggg acgggtagtt
       61 tgccgctctc gatatgccgg acggagccaa ggccatcgat cacggggcgc gtcggtggaa
      121 gaacccgcgc ctgcgtgacg tatgcgtctt cgccgcacca tctcacccgt cacgccacca
      181 cataccggga actgccgcca aggtctaact caaccaatct agccgcgcta catacggttt
      241 ttgtggcaac tcatcaggtg tccgctgtcg atgaaactct ctgcgccggt agcggaactc
      301 tcacgcgtgc ggttgccgtc gtcacagctc ccccaaccgc aaacggtctt tgcaaacacg
      361 caatcatctg caaaaacagg attttttcgg gattctgggg aggctgtgcg gtcggtaatg
      421 cttgaagaat acagaaggtt catgcaaact gaatgaactg gtttccgttg tcactgcaac
      481 agactccaca ggaacattga ggaggtcgaa aacctcaatg aacccgaaga agctcgccta
      541 acggcgggct tttttcgttc tacgctccct gacgaaagat cgcaagcaca ataacgcgat
      601 ttttcacgct tttacgctca aaacatcaaa aaaccatgct ttcccccacc cctacctcaa
      661 aaaacgcgtc agaaacgttt ccaggggctt gcataactat tcacaaaagt gattcggaat
      721 caatttcgcc aaatctgagc accgaacggg tcaaaacggc aaactttctc atcagaaaac
      781 ggtgaacggg acgcggaatt ttttcgcgtc ccgtgaggcg ttggacaagc gaagcgcgtc
      841 agtttttccg gctcttccct gttgctggtt tctccgccag tttatcttcc agtgtcacaa
      901 ctttctgata caccgccgct gtggcgttgt agttcctgaa acagcaaatg ttagccatcg
      961 cgacccggga agggtttgaa gtgaataaac tgatttgggt gaaacagcct ggcgcttagt
     1021 gagtgctcag cttcagacca ataatgccag caacgatcag cccaaggctc agcaaacgtg
     1081 ccgggctggc agactcaccc agcagcaaaa tccctgtaat ggccgcccca acagcgccaa
     1141 taccggtcca gaccgcataa gcggttccta caggcaacgt gcgcattgcc caagagagca
     1201 tggcgatact gacgatcatc gccgcaatag tgataatgct tggcgtaaga cgcgtaaaac
     1261 cgtgggtgta tttcaggcca atcgcccaga caacttcgag caaacctgca attaataaaa
     1321 cgatccagga catatcaggc tccagaacaa tgggtccgtc cccggtgaaa gaagcgtttg
     1381 caggtcgtcc tgcaaagcta atgtgtgaaa tggcattttt gcccggaaga aaatgaattt
     1441 caagagcatt gttttcattc cccatccaag cacacagaac ataaccagaa aagccactga
     1501 caccccaccc agccatttcc agctataccc tttaagggtg ttgtattcct gcacgatatt
     1561 gctgtgaatt gtcgcaatat ccctgttcag ggcatccgcg tttgccttca tgtttgcgac
     1621 tgactcttcc agccgctgaa aatcctggct gatgcgctca cctatcagcc ctgcaacatc
     1681 cagcttctgc acctcgtttc tgacttcatc cctgacctgt tttgccacac cgtcagaaat
     1741 atcccgatat ccgcgaagtg tttcccttgc cacagccaga gaatcagcaa cagctttctg
     1801 ttgctccatt gcggtatgtt gcagatgctc caccgataac aggagcgtga gtaatttttc
     1861 ttctgtgctg gccatgttca gaatcccata gaaaatccac gtgacggtga tttaacccgt
     1921 tcaggttcag ttcggttctg tctgctcact ctgtctctca tgtcaaagcc atgctcccgc
     1981 gcaacatcat gttgcagcct tctgaccagt tcatgttgct taaccggagt gtccagccat
     2041 tccctgatgt catgcccgac cgttcgcgcc tgttcaagat ggcgggaata gtccggttca
     2101 gaccgttttt gcatgatgtc atgacgctgg gcatcatcaa gccgtccggc agcttcaaaa
     2161 cccagatgca cttcgggacg tcgttcaatg ccctgttctt tcagcgtccg gcagtcaatg
     2221 aagccccttt caaagccatg ctttctcata tgttcgttgt gcttcacctc ccagcgtttt
     2281 cgcaattccc gcaactgctc tttttgctga gtgagagtca ggcttccgga atcctttttc
     2341 gcacctcccc gctcgggata cctggcgtta tagcgccgga aatactgttc cggtgtacgt
     2401 tcaatgccat cattcacacg ctggctcatc atgatatgcg catgaggctg ttcgccgccg
     2461 tcaatgctcg ctttcggatt gtgaatggca aaactccagg cgtgccgttc gccagcttcc
     2521 tgacgtacaa aatcctgcac cagttcaagg cgctgttccg ctgtcagttc acggggtaat
     2581 gcaatttcaa gttcccgata ggtcgaaccg ttcgcacgtt caaactggtc agccgcctgc
     2641 cagaaatgtg agggattatc tcgcgcccat tccggcatgt ttccgtgtgc ggaatattcc
     2701 aaatcctgac ggtcgcggta tttctctttg cgctcgatgt aatcggcgtg gtttgcagcc
     2761 tgccccttgc caccaaattt aacgctgagg tgataggttg ccacggtttc agccctccgc
     2821 aggagcgcac tgtttctcga cgaaggagag aaacatataa gtgcgcttcc ttctaatttt
     2881 acgaattagc acggaagata ccccctgcac ccaccgaccg caagcgatat actgcacaac
     2941 cccaacacac cgcaaaaaac accagggagc atcccaaaca tgcgcatcag caacatcgac
     3001 tggttgaaga aacgaatcgg ctttatcagg aagctcggag aacagaccgc acgacagagg
     3061 cagatcattg acctgctcga taacgaagcc ggactaacag aacaggagcg caaactactc
     3121 cacgtcctgg caaccgcaga aaaaaacgag ctacaggcac aggagaacgc acgcaaacag
     3181 gccaaccaaa aacggatgga ggggaaaaca caacgacggg aacgcaatca ccgcctgttt
     3241 cttgcggcgg ggttactgat cgaggccgga ctagtggaca caaaaacagg cgaactgaga
     3301 taccaaaaag acaatatatt acagaaactt aaggcaatca ggctcgatct tgaaacaagc
     3361 ccagatcatc attaattccg ctgcgtggtg gtgcgtttgc ctgccggtgt tcaatgtttt
     3421 cgatttcgcg acgcgctacg cgcggatctg gcataggtgc gtacgtgctc aatgtggttt
     3481 ccggttcact tccgcttcct cgcccactcg ctcactacgt tcggtcgctg gggctgcggc
     3541 gagcggtggg atcactctca catgatgcag atcctttttt tttttgctct ggagttatca
     3601 gttgttgcgg tgctcatggg atcgtgcgat atgctgga
//
