LOCUS       NZ_CP042890             4057 bp    DNA     circular CON 13-JUN-2023
DEFINITION  Escherichia coli O10:H32 strain NMBU-W12E19 plasmid pNMBU-W12E19_06,
            complete sequence.
ACCESSION   NZ_CP042890
VERSION     NZ_CP042890.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12565117
            Assembly: GCF_013282315.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O10:H32
  ORGANISM  Escherichia coli O10:H32
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Finton,M.D., Meisal,R., Porcellato,D., Brandal,L.T. and
            Lindstedt,B.-A.
  TITLE     Multidrug-resistant environmental bacterial isolates, Norway
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4057)
  AUTHORS   Finton,M.D., Meisal,R., Porcellato,D., Brandal,L.T. and
            Lindstedt,B.-A.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2019) Faculty of Chemistry, Biotechnology and Food
            Science, Norwegian University of Life Sciences, Chr. Magnus
            Falsensv. 1, Aas N-1430, Norway
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 06-AUG-2019
            Assembly Method                   :: Unicycler v. 0.4.7.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 34.78x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/13/2023 04:17:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,556
            CDSs (total)                      :: 5,427
            Genes (coding)                    :: 5,049
            CDSs (with protein)               :: 5,049
            Genes (RNA)                       :: 129
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 126 of 378
            Pseudo Genes (incomplete)         :: 250 of 378
            Pseudo Genes (internal stop)      :: 61 of 378
            Pseudo Genes (multiple problems)  :: 56 of 378
            Pseudo Genes (short protein)      :: 5 of 378
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP042890.1.
            Bacteria and source DNA available from Prof. B-A. Lindstedt, KBM,
            Norwegian University of Life Sciences, P.O.Box 5003, N-1432 Aas,
            Norway.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Escherichia coli O10:H32"
                     /mol_type="genomic DNA"
                     /strain="NMBU-W12E19"
                     /serotype="O10:H32"
                     /isolation_source="surface water"
                     /db_xref="taxon:2603836"
                     /plasmid="pNMBU-W12E19_06"
                     /geo_loc_name="Norway:Aas"
                     /lat_lon="59.667 N 10.769 E"
                     /collection_date="2019-02"
                     /collected_by="Cecilie Fagerland"
                     /genotype="ST1286"
     gene            join(2261..4057,1..351)
                     /gene="mobQ"
                     /locus_tag="FVP48_RS02335"
                     /old_locus_tag="FVP48_02635"
     CDS             join(2261..4057,1..351)
                     /gene="mobQ"
                     /locus_tag="FVP48_RS02335"
                     /old_locus_tag="FVP48_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_077124374.1"
                     /translation="MAIYHLSMKIISRSNGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGESGLAALRSQL
                     LKREEELRRKELEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPSNGEMTSYLL
                     LHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQHPPLQRIYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKGLRYA
                     GYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRTWDEW
                     RREEDARTHATERRHDRGWSQ"
                     /name="mobQ"
     gene            complement(590..1297)
                     /locus_tag="FVP48_RS02340"
                     /old_locus_tag="FVP48_02640"
     CDS             complement(590..1297)
                     /locus_tag="FVP48_RS02340"
                     /old_locus_tag="FVP48_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296720.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_077124375.1"
                     /translation="MTSNPLIAYKSNALVEASYKLTLQEQRFLLLCISRLNSGTDVASP
                     ELQKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRA
                     QYTRGEGKAQITFSDAVMPYLTQLKGQFTRVVIKNISNLSRSYSIRIYEILQQFRSTGE
                     RIIALDDFKSSLMLDGKYKDFKTLNRDLIKPCVDELNKKSDLAVTVETIKKGRTVVALH
                     FRFKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(1828..2082)
                     /gene="mobC"
                     /locus_tag="FVP48_RS02345"
                     /old_locus_tag="FVP48_02650"
     CDS             complement(1828..2082)
                     /gene="mobC"
                     /locus_tag="FVP48_RS02345"
                     /old_locus_tag="FVP48_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091744.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     Typing          complement(2084..2260)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="1.22e-84"
     Typing          2261..2619
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.056"
                     /coverage="100.0"
                     /evalue="3.94e-177"
     Typing          complement(592..1297)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.717"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          2175..4057
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.994"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggtgttgtcc ggcttaatgg tacgctgtat cccgcgtttg ccgttccctc agccgataac
       61 agccagctgg ttatcagtgc actgaccgat aaggggttac ggtatgcagg gtatggtgtt
      121 gccgtaaatc atgatgcaga cagccacata tcacctgcgc cggaacttat ggagtttcat
      181 ctgaaaaccc gtgaggcacc gctttttgct gccgtcaaca caccagaaaa acagccggat
      241 catctgttcc gctcgctggg tttcaatcgt acctgggacg aatggcggcg cgaggaagat
      301 gcacgaacac acgccaccga gaggagacat gatcgcggtt ggtcacagtg aatgtgaaca
      361 atattttgtt ttgtttagca tcgtgttatg ttcatcgaga gggatgaatg caagaagtac
      421 agtcacataa cgcaaacata gttttgtttt tccgcccccc aaaaattggg ggttcttttt
      481 tcgctgcatc agagcgtttt tgacgcaatt ttttacttca atagtacctt cgcactacta
      541 acacaaaaaa aacgcagcag ggagcgtttt aggtgcctta aaatcgattt tagatcgtca
      601 tcttgatctg tttgtcttcc ttgaagcgaa aatgcagggc tacaaccgtg cgcccctttt
      661 tgatcgtctc aaccgttaca gccaggtcgc tttttttgtt cagttcgtca acacagggtt
      721 ttattagatc cctgtttaac gttttgaaat ctttatattt accatcaagc atcagtgatg
      781 atttaaaatc atcgagggcg ataatacgtt ccccagtact gcggaattgc tggagaattt
      841 cgtatattct tatgctgtaa cttcttgata aattggaaat atttttaatg acgactctgg
      901 tgaattgccc cttcaactgt gtcagataag gcattaccgc gtcagaaaaa gtgatttgtg
      961 ctttcccctc tcccctcgtg tactgtgccc gatactgaat ccagcggaac tcctcgcgct
     1021 tctcgtcgtc cttgagaatg atcgacctgt cccacaaacg atcgatcgct tcctgtagct
     1081 gaacctcggc atttttccgc cccatatcag ggaatgaatc aaaatattcc gcagctgtaa
     1141 tggtcattgt cttttgcaat tcaggtgaag ctacgtcagt accggagtta agtcggctta
     1201 tgcagagcaa taagaaacgc tgttcttgta aagttagctt gtaactggcc tcaaccagcg
     1261 cattggactt ataggctatc agagggtttg atgtcatttt aaccaccagg ccaaaaatga
     1321 ggtgagataa aaacctatca cctttagcat tggtgagcaa ttatatttcc accccccatt
     1381 ttcctgtgaa tattacctta aatctcctca cctttacctt aaattacctc acccaagcac
     1441 cttaaattat atcaccttta ccttaaattc ccccacctat acacgtgtaa ctcattgttt
     1501 ttaatcagga atttcgaccc taaaacaagt attaaaaaca agatcttaaa aacaagatct
     1561 aaaaacttaa cggcgagcct gtggataact tggggtgctg aaaataaaaa tccaataaaa
     1621 acaatgtgtt gcaaaaaatc aggattgaag attccgaaca cagcccctcc aaccctgtgg
     1681 aaatccagta ttcatgcggg ttcagcgcat atcacctgtc gaagaaccgt gcgtatgtgc
     1741 tcaacatggc gactgattca cttccgcttc ctcgcccact cgctcgctaa cgttcggtcg
     1801 ctgggctgcg gcgagcggac gatacaatca ctgctctggc agcgcctcca gctcgttaat
     1861 cagcggcagt attcgcgtca catcagcctg ccgtgtcacc tctgccttca ggaactggag
     1921 taaccaggct cgcaactgca cttcctccgg ctttctgacc tttgccagca atgctgcacc
     1981 cacaatgacc ttctgcgctg tgtccaggct ccggctcttc gccttcaggc gctgtaatct
     2041 cgcctcagcg tcagcaatct tctgttcgag cgttctgctc atgatgtaac tccgtgcgcg
     2101 atgaaaaatc gcattttagc gcgtcactgg tagtttaaaa actgagctgg cataatgcac
     2161 ggaaaatcac gaagtgcgca cttatacaat ctccactccg tttcgattgt gtgcgccctg
     2221 cggggctgaa agaaaactgc aaaaaggcat tacggcagaa atggcgattt atcatctcag
     2281 tatgaaaatc atctccagaa gcaacggcta cagcgccgtt gcttctgctg cctatcgttc
     2341 cggttcactg atgcttgatg agcgtaccgg actgactcac gactacaccc gcaaaagcgg
     2401 tgtggctgag gctgtgattc tgacccccgc gacagcccct gcctggtgta cgaaccgtgc
     2461 cgaactctgg aatgccgttg agaaggccga acgccggaag aactcacaac ttgcgcggga
     2521 aattgaactc gccatccccc gtgaactgcc gcaggatgcc gcacgggaaa ccgttctcgc
     2581 ctttgtcaga gaaaactttg tcagtcaggg catgattgct gatgtggcgt tccatcacat
     2641 ggataaaaca aatccccatg cgcacatcat gctgaccacg agggctgtcg ggcctgctgg
     2701 gttcggcgga aaggtcaggg actggaacga ccggacacat gccgagacgt ggcgtgcatc
     2761 atgggctgac catgcaaaca gggcactggc gaacgccggt tatcaggaag agatagacca
     2821 tcgttcatac gagcgacagg ggcttgagaa aacgccgggt attcacctcg gaaagtctgc
     2881 ctgtgcgatg gaaacacgcg gtatcgaaac agaacgcggc gaacagaatc gtctgattaa
     2941 caggcttaac ctggaaatac agatttcccg caccgaactc aggaacagcg ggctgacaga
     3001 gcctgcccgc cgtgtggctg accttctgaa tatcgggctt cctgacaacg caacttcgta
     3061 cacactacgt gacatcatcg aagcgttgcc gaaggacagt aacgcggcat ggaaactcac
     3121 cagtaacgcc atgtcgatga tggcagatat gcaggcgacg cgcagacgct gggaagagct
     3181 gaacaccgaa cgaaaggaag cgatgagcca tgctgccagc ctgaaaaaat catcgccgtt
     3241 ttcgtccggt ttttcccgta ttcccctgat gcaatgggct gcaccggaat accgtagtga
     3301 gcagggaaaa atccagtctc tgagccagca aatggaaaaa ttgcgacagc attaccggaa
     3361 ggtggagaaa caggacattc ccgcgagtca gacggcattt gaaaaacagt ggcagcaatg
     3421 gggggaatcc ggccttgcag ctctcagatc ccagttgctt aaacgtgaag aggaattgcg
     3481 ccggaaggag ctggaggata cagaacgtag tcgcgcggag cagctcagga aaaatgacaa
     3541 tgccgttatg gattcagggg tgctgagagc agtcgttacg gggtatggcg aggctcccat
     3601 gcctagtaac ggggaaatga ccagttacct tctgcttcac aaccggaacg gagaatatac
     3661 gctctggggg aacgagctgg agaaataccg caccaggatc tttgagagtg ttgatttaat
     3721 gcgtgacagg agtggttata tctgtgaccg aagcgaaatc ggtcaacatc caccactgca
     3781 acgtatttat tcctctgcga cctttgagca acttctgacg caggtttgcc agacatggcc
     3841 gcaacatacc cgcaatctcc gccagccgaa gacatggccg gagtctttct gtcttggaga
     3901 agaccgccag ccagccatgc catcactggc agcaaggaag gtggatttta cgcagggacg
     3961 tctgccaccg acgctgatgc cggtcatgag ttctgtcgac cgggaaaccc gccagcttca
     4021 gcttttgctg gtcatgggcg tggatgattc tctgggc
//
