LOCUS       NZ_CP042889             7939 bp    DNA     circular CON 13-JUN-2023
DEFINITION  Escherichia coli O10:H32 strain NMBU-W12E19 plasmid pNMBU-W12E19_05,
            complete sequence.
ACCESSION   NZ_CP042889
VERSION     NZ_CP042889.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12565117
            Assembly: GCF_013282315.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O10:H32
  ORGANISM  Escherichia coli O10:H32
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7939)
  AUTHORS   Finton,M.D., Meisal,R., Porcellato,D., Brandal,L.T. and
            Lindstedt,B.-A.
  TITLE     Multidrug-resistant environmental bacterial isolates, Norway
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7939)
  AUTHORS   Finton,M.D., Meisal,R., Porcellato,D., Brandal,L.T. and
            Lindstedt,B.-A.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2019) Faculty of Chemistry, Biotechnology and Food
            Science, Norwegian University of Life Sciences, Chr. Magnus
            Falsensv. 1, Aas N-1430, Norway
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 06-AUG-2019
            Assembly Method                   :: Unicycler v. 0.4.7.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 34.78x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/13/2023 04:17:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,556
            CDSs (total)                      :: 5,427
            Genes (coding)                    :: 5,049
            CDSs (with protein)               :: 5,049
            Genes (RNA)                       :: 129
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 126 of 378
            Pseudo Genes (incomplete)         :: 250 of 378
            Pseudo Genes (internal stop)      :: 61 of 378
            Pseudo Genes (multiple problems)  :: 56 of 378
            Pseudo Genes (short protein)      :: 5 of 378
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP042889.1.
            Bacteria and source DNA available from Prof. B-A. Lindstedt, KBM,
            Norwegian University of Life Sciences, P.O.Box 5003, N-1432 Aas,
            Norway.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7939
                     /organism="Escherichia coli O10:H32"
                     /mol_type="genomic DNA"
                     /strain="NMBU-W12E19"
                     /serotype="O10:H32"
                     /isolation_source="surface water"
                     /db_xref="taxon:2603836"
                     /plasmid="pNMBU-W12E19_05"
                     /geo_loc_name="Norway:Aas"
                     /lat_lon="59.667 N 10.769 E"
                     /collection_date="2019-02"
                     /collected_by="Cecilie Fagerland"
                     /genotype="ST1286"
     gene            join(7399..7939,1..1052)
                     /locus_tag="FVP48_RS02285"
                     /old_locus_tag="FVP48_02560"
     CDS             join(7399..7939,1..1052)
                     /locus_tag="FVP48_RS02285"
                     /old_locus_tag="FVP48_02560"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_001009226.1"
                     /translation="MNFIEKYIITFNKCNLFFIILLSTISSFLFVLFGYLIKTVIDNKD
                     SIGEINSLLIFIACFLSIRFLMPAGYSISEYLTQKTNIELSVKLREQVIDNIQNSHQEH
                     FLKKNKGELNKVIESMLSSASSLFYTICSDVVPLLIQMIGIIITICISVNTLIAIEFII
                     IMAIYIIFVIKMTQRRFPMMKSVALSSKFASGRMFDMMHMYPMDKAFHTTDKSRKRVIQ
                     AVNTHSEKQRKVNNEFFLFGISSAFLSVIFSSLIILSAYWMFLHGRASRGSIIMLATFL
                     FQVFLPLNRIGYLFRQIKMARTEIDLYCAEINDIKKTNYTDKQYLHVKDNICNIEIYNK
                     SFNHKLILTKGVVTFITGENGSGKTTIAKILSGNITTEENTIKINGIQQEKANSPFVNV
                     LYVPQDLDLMPGTIEENILHYSGINNLNTIKKLLHRFKFDKPLDYEIKGYGHNLSGGQK
                     QKLGVSLTSGKNVDLIIFDEPTKGFDSLGIKIISDYIKEENKEKHIIVISHDEQLINNI
                     PKAQIIDITKHEV"
                     /name="ABC transporter ATP-binding protein"
     gene            1055..1948
                     /locus_tag="FVP48_RS02290"
                     /old_locus_tag="FVP48_02565"
     CDS             1055..1948
                     /locus_tag="FVP48_RS02290"
                     /old_locus_tag="FVP48_02565"
                     /inference="COORDINATES: protein motif:HMM:NF019619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="WP_001061104.1"
                     /translation="MNNLIKKEIIEKFKKYNFQKYPFVFTDVNYKNLINWNDLNKLLEK
                     DILHYPRVRMANDNFPEIRGYKGFIRYTYSQTGDRTPHINRHQLYKCLRDGATLIVDRC
                     QSFFESVDESRLWLSKELECTCSANLYAAFTATPSFGLHFDNHDVIAVQIEGIKKWKVY
                     NPTYSYPLEDERSFDYLPPNTSPDYEFDITPGQAIYLPAGYWHNVTTQSKHSLHISFTV
                     IRPRRLELFKTLFDELKNNPYMREPIEHGDSLSDKEKIKTIITNAINSFDIQFAENMLK
                     AQSRTYRYKKIKLEHI"
                     /name="cupin domain-containing protein"
     gene            complement(1973..2335)
                     /locus_tag="FVP48_RS02295"
                     /old_locus_tag="FVP48_02570"
     CDS             complement(1973..2335)
                     /locus_tag="FVP48_RS02295"
                     /old_locus_tag="FVP48_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="WP_000181070.1"
                     /translation="MTNFKSDLNLIRKEWYNAFYSGNTEMLDYLEADWFISTNGQKVIY
                     KKHQLRKIDMRPARILPSIERYEYDVVMREFKGIACVSGKAEIKQNDGNILMAFIENWI
                     KVNGGWKMQFISYEKI"
                     /name="DUF4440 domain-containing protein"
     gene            complement(2332..2571)
                     /locus_tag="FVP48_RS02300"
                     /old_locus_tag="FVP48_02575"
     CDS             complement(2332..2571)
                     /locus_tag="FVP48_RS02300"
                     /old_locus_tag="FVP48_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2164 family protein"
                     /protein_id="WP_000703021.1"
                     /translation="MKISDIEKNIYKGLAEFISQKIRDEHDICLGQFESEELLDEILKR
                     VIPVIYNVAIDDAIKSVRNVGDRLEEELDMRKVV"
                     /name="DUF2164 family protein"
     gene            complement(3282..3515)
                     /locus_tag="FVP48_RS02305"
                     /old_locus_tag="FVP48_02590"
     CDS             complement(3282..3515)
                     /locus_tag="FVP48_RS02305"
                     /old_locus_tag="FVP48_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137439.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3522..4040)
                     /locus_tag="FVP48_RS02310"
                     /old_locus_tag="FVP48_02595"
     CDS             complement(3522..4040)
                     /locus_tag="FVP48_RS02310"
                     /old_locus_tag="FVP48_02595"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001673203.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVLFLFTVSG
                     GTLWYLGGMIQANLEEIRIQEETLQKLNAKTWGVEFVQDGRRKFLVIPQGKSATVIPYQ
                     GKDWVQLTE"
                     /name="MbeB family mobilization protein"
     gene            complement(<4078..4509)
                     /locus_tag="FVP48_RS27765"
                     /pseudo=""
     CDS             complement(<4078..4509)
                     /locus_tag="FVP48_RS27765"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4718..5041)
                     /locus_tag="FVP48_RS02320"
                     /old_locus_tag="FVP48_02605"
     CDS             complement(4718..5041)
                     /locus_tag="FVP48_RS02320"
                     /old_locus_tag="FVP48_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955993.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVLAALMAIGEELRRLRLAVREQGAR
                     DDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5106..5297
                     /locus_tag="FVP48_RS02325"
                     /old_locus_tag="FVP48_02610"
     CDS             5106..5297
                     /locus_tag="FVP48_RS02325"
                     /old_locus_tag="FVP48_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            6662..7360
                     /locus_tag="FVP48_RS02330"
                     /old_locus_tag="FVP48_02630"
     CDS             6662..7360
                     /locus_tag="FVP48_RS02330"
                     /old_locus_tag="FVP48_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000939065.1"
                     /translation="MLQHKMNSSSYAKVHNVSSLEDIMSYHNDDVLLKFRKEWNVTPEE
                     ADDIFNETKKFIWLASTCLTECYNIKVHEQLQIIDEMWHTFIQFTDAYTSFCEKYLGAY
                     LHHYPNTNDMLKNEIRHVNEHGITFQEYRFNEYKNQIEKIAFYLGHETVAKWYGDYAVR
                     YSIKNINTIRIPKESISSDSYIEKVKSITHLPAAEFVKIIMRKDVWNDNGSVCGCSGKG
                     CGAGCSCNSR"
                     /name="hypothetical protein"
     Typing          complement(5374..5453)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.78"
                     /coverage="92.0"
                     /evalue="8.94e-33"
     Typing          5486..6028
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.679"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          complement(3179..4728)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.787"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4725..5074)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.872"
                     /coverage="99.0"
                     /evalue="9.42e-67"
ORIGIN
        1 caatgatgaa atcagttgca cttagctcca aattcgcatc tggaagaatg tttgatatga
       61 tgcacatgta tcctatggat aaagctttcc ataccacgga taaaagtcgt aaacgagtaa
      121 ttcaagctgt aaatacacat tcagaaaaac aaagaaaagt aaataatgaa tttttcctgt
      181 ttggtatcag ctccgctttt ctttcagtca tttttagtag tctgattatc ctatcagcat
      241 actggatgtt tcttcatggt agagcaagcc gtggtagtat tattatgctt gccacttttt
      301 tattccaagt ttttctccca ttaaatcgca ttggttatct atttagacaa ataaaaatgg
      361 caagaacaga aattgattta tactgtgccg aaataaacga cataaaaaaa acaaattaca
      421 cagacaagca atatcttcat gtaaaagaca atatttgcaa tattgaaata tacaacaaat
      481 cattcaacca taaattaata ttaacaaaag gcgttgttac ctttataacc ggtgagaatg
      541 gttcgggaaa aacaactatt gcaaaaattc tctctggcaa tataacaaca gaagaaaaca
      601 caataaaaat caatgggata caacaagaaa aagcaaattc tccttttgtt aatgtcttat
      661 acgttcctca agatctagat ctcatgcctg gaactataga ggaaaatata ttacattatt
      721 ccggcattaa taatttaaat acaattaaaa agctgctaca cagatttaag ttcgacaaac
      781 cactagatta tgagatcaaa ggttacggac ataatttatc tggtggacaa aaacaaaaat
      841 taggagtatc tcttacatct ggaaaaaacg tagacttgat tattttcgat gaaccgacaa
      901 aagggtttga ttcacttggt ataaaaataa tcagtgatta tataaaagag gaaaacaagg
      961 aaaagcatat cattgttata tctcatgatg aacaacttat aaataatata cctaaagcac
     1021 agattataga tatcacaaaa catgaggttt aaaaatgaat aatcttataa aaaaggaaat
     1081 catagaaaaa tttaagaaat ataatttcca gaaatatccg tttgtattca cggatgttaa
     1141 ttataaaaac ttaatcaact ggaatgatct taataaattg cttgaaaaag atatattgca
     1201 ctatcctaga gttagaatgg caaatgacaa ctttcccgaa attagggggt ataaaggatt
     1261 tataagatac acatatagcc aaacaggcga tagaacacca catataaatc gccatcagct
     1321 atataaatgc ttacgtgatg gcgcaaccct tatagtagat cgatgccagt cattctttga
     1381 atctgtagat gaaagcagac tatggttatc taaagagtta gaatgtacat gtagtgctaa
     1441 tctatatgct gcatttacag caacaccaag ctttgggctt cattttgaca atcatgatgt
     1501 aatagctgtt caaattgagg gaataaaaaa atggaaagtt tacaacccta cctattcata
     1561 ccctctcgaa gatgaaagaa gcttcgatta tctaccacct aatacctccc cagattatga
     1621 gtttgatata accccaggtc aggccatata tcttcctgct ggatactggc acaatgttac
     1681 gactcagagc aaacactctc ttcatatatc ttttacagtt ataagacctc gtcgattaga
     1741 actatttaaa acattatttg atgaattaaa aaacaatcca tatatgcggg aacctataga
     1801 gcatggtgat tcattatctg ataaagaaaa aataaaaact ataataacta atgctataaa
     1861 tagttttgat attcagtttg cagaaaacat gcttaaagct caaagcagaa cttatcgcta
     1921 taaaaaaata aaacttgaac atatataacc attaatctaa gggggcttcc ccttagattt
     1981 tttcatacga tataaactgc attttccatc caccattaac ctttatccaa ttctcaataa
     2041 aagccattaa tatatttcca tcattttgct ttatttcagc tttccctgaa acacaagcaa
     2101 tgcctttaaa ctcacgcatt acgacatcat attcatatct ttctatgcta ggtaaaatcc
     2161 tcgctggacg catatcaatc tttcgtaact gatgtttttt atagataact ttttgaccat
     2221 ttgttgaaat aaaccaatca gcttccaagt aatctagcat ttcagtatta cctgaataaa
     2281 atgcattata ccattctttt cttattagat ttaagtctga tttaaaatta gtcacactac
     2341 cttcctcata tctaattcct cctctaatct atccccaaca ttccttactg attttatagc
     2401 atcatcaata gccacattat atatgactgg gattactctt ttaagaatct catcaagtaa
     2461 ttcctctgat tcaaactgtc caaggcatat atcatgttca tctctgattt tttgtgaaat
     2521 aaattcagct agtcctttat atatgttttt ttcaatatca gaaattttca ttctttatga
     2581 agcctcacaa ataaattaat aatataacat ttttcctatc ataggaaaat tacaactcaa
     2641 ccatactgca acctggaatt tcccaagcaa gcatatttaa ataagtgcag gataccacct
     2701 caaatcattc tctaaggaac aagataattt accataatcc cttattgtac gcaccgctga
     2761 aacgcgttcg gcgcgatcac ggcagcagac aggtaaaaat ggcaacaaac cacccgaaaa
     2821 actgccgcga tcgcacccgg taaattttaa ccacatgcat agctatgcag ccatgtgaat
     2881 cacgcaggaa ttgccagcca gagacagctg aaacggattt ttttcatgct ttcggaagcg
     2941 aagaagacgg ggaccggagc gggaaaacag aagttcaggg gaatgagcgc gatctggcaa
     3001 tagaggcaaa acacagcaac aaaagacaca ccagaatcgc gcccgtatgc gttttaacgc
     3061 gttcctgtgt tttcagcaag gtctgactga agcgcgttcc cggtgccccc gaaccaacca
     3121 ttatcccaaa aaatgcaccg gaaacgcgct gacaacattt tagcctgtct ctgattacca
     3181 tcccagcgaa gggccatcct gtgtccgttc ctgtatttcc agctggcgct cacgctccag
     3241 ggataactca tgttcgcgtt cctgtatcag ccgttcatcc cttatctcct gttctcgctg
     3301 tataactggc tcaagcgttc tgtctgctcg ctcaagtgct gcacctgctg actcaactgc
     3361 atgacccgct cgttcagcat cgcgttgtcc cgttgcgtaa gcgaaaacat tttctgcaat
     3421 tccacgaagg cgctctccca ttcgttcagc cgctgcatat agtcctgttg cagttgctct
     3481 aaggcgttca gcaaatgtct ttccagctcc gtcactctgt gtcactccgt cagttgtacc
     3541 cagtccttcc cctgataggg aatcaccgtt gccgatttcc cctgagggat aaccagaaat
     3601 ttccttctcc cgtcctgcac aaactccacg ccccatgtct tcgcgttcag tttctgcaat
     3661 gtttcttcct gtatcctgat ttcttccagg ttcgcctgta tcatcccgcc aagataccag
     3721 agcgtcccgc cactcacggt aaacaggaaa aggaccatcc ccagtaacat catgcccgta
     3781 ttccctgcca gctttaacac gtccttcctg tgctgcatca tcgcctcttt caccccttcc
     3841 cggtgttttt tcagtgattc ctctgtcgaa gctgtgaaca gggctatagc gtctttgatt
     3901 ttcgtctcgt ttgatgtcac agccttgctt acagattttt cgagcttgct gaactcgtcg
     3961 ttcagcattg tctctgtaga ttcggctctc tctttcagct ttttctcgaa ctccgcgccc
     4021 gtctgtaaaa gattgctcat aaaatgctcc tttcagcctg atattcttcc caccgttcgg
     4081 gtctgcaatg ctgatactgc ttcgcatcac cctgaccact tcaagccctg cctctgtgag
     4141 cgcctgaatc acatcctgac gaccttttat ctccccgaca tgataaagag cgtctatccc
     4201 gcgtgtgacg ctctctgcaa gcgcctgttt cgtttccggc aggttatcag ggagagtcag
     4261 tatccggcgg ttctccgggg cgttggggtc atgcagcccg taatggtgat tcaccagcgt
     4321 ctgccaggca tcaattctcg gcctgtctgc ccggtcgtag tacggctgga ggcgttttcc
     4381 ggtctgtagc tccatgttcg gaatgacaaa attcagctca agccgtccct tgtcctggtg
     4441 ctccacccac aggatgctgt actgattctt ttcgagaccg ggcatcagta cacgctcaaa
     4501 gctcgccatc accttttcac gtcctcccgg cggcagctcc ttctccgcga acgacagaac
     4561 acctgacgtg tatttcttcg caaatggcgt ggcatcgatg agttcccgga cttcttccgg
     4621 attaccctga agcaccgttg ccccttcccg gtttcgctcc cttcccagca ggtaatcaac
     4681 cggaccactg ccaccgcctt ttcccctggc atgaaattta acaatcatcc cgcgcgccct
     4741 gttccctgac ggccagacgc agccgacgca gctcctcccc gatggccatc agcgcagcca
     4801 acacctgaac ccgatcaccg gaagaccact gcccgctgtt caccttcctc gctgtctgat
     4861 tcaggttatt cccgatggcg gccagctgac gcagtaacgg cggtgccagt gtcggcagct
     4921 tcccggagcg tgcaaccggc tcacccaggc agacccgccg catccatacc gccagctgtt
     4981 taccctcaca gcgttccagt aaccgggcat gttcatcatc agtaacccgt atggtgagca
     5041 tcctctcgcg tttcatcggt atcattaccc cataaaacag aaatccccct tacacggagg
     5101 catcagtgac taaacaggaa aaaaccgccc ttaacatggc ccgttttatc agaagccaga
     5161 cattaacgct gctggagaaa ctcaacgaac tggacgcaga tgaacaggcc gatatttgtg
     5221 aatcacttca cgaccacgcc gacgagcttt accgcagctg cctcgcacgc ttcggggatg
     5281 acggtgaaaa cctctgacac atacagctcc cggagacggt aacagcttgt ctgtgagcgg
     5341 atgccgggag cagacaagcc cctcagggcg cgtcaggggg tttcggggcg aagccctgaa
     5401 ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc agtacgaatt
     5461 gtatggaaag cgcaccatgt ccgatgtgaa atgccgcaca gatgcgtaag gagaaaatgc
     5521 acgttccggc gctcttccgc ttcctcgctc actgactcgc tacactcggt cgttcggctg
     5581 cggcgagcgg tgtctgctca ctcaaaagcg gagatgcggt tatccacaga atcaggggat
     5641 aacgccaaaa gaaacatgtg agcaaaaaac gagaaccagg aaaaggctgc gccgttggcg
     5701 tttttccata ggctccgccc ccctgacgag catcataaaa atcgacgctc aagtcagagg
     5761 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     5821 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     5881 agcgtggcgc tttctcatag ctcacgctgt aggtatctcg gctcggtgta ggtcgttcgc
     5941 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     6001 aactatcgtc ttgagtccaa cccggtaaga cacgacaaag cgccactggc agcagccact
     6061 ggtaactgga caaggtggat tgagatatta agagttcttg aagtggtggc ctaactgcgg
     6121 ctacactaga aggacagtat ttggtatctg tgctccacca agccagttac ccggttaagc
     6181 agtccccaac tgacttaacc ttcgactaaa ccgcctcccc aggcggtttt ttcgtttact
     6241 ggcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc tttttgagat
     6301 ccgcccactc ctggaaccta caacacatgt gtaaaacgtc aatggagggg gctattatcg
     6361 gactgcaaca catttcgtac agcctttaca ctcggtgaat tagcggccct agatgcatcc
     6421 actcattcaa ccaactcaat tctcttctct tagagtggga aaattttgtt ttccttgcag
     6481 cccctacccg taaaaactgg ctgcaaaaac cttgtattac taatggatcc tataaccatt
     6541 aaaaaacttg attactatct catttataga attaggcata ttgacacaag cacaaattta
     6601 acaataccat aacaaatgat aactattctc atctacattc aaatatataa ttgggggtgt
     6661 tatgctacaa cataagatga acagcagttc ttatgcaaaa gttcataatg ttagctcatt
     6721 agaagatatc atgagttatc acaatgatga tgttcttcta aaatttcgta aagaatggaa
     6781 tgtaacacca gaagaagctg atgatatctt taatgaaaca aagaaattca tctggctagc
     6841 ctcaacatgc ctaacggaat gctacaatat aaaagttcac gagcaattac aaattataga
     6901 tgaaatgtgg catacgttta ttcaatttac agatgcttat accagctttt gtgaaaaata
     6961 tcttggtgct tacctccacc attatccaaa tacaaatgac atgctaaaaa atgagataag
     7021 gcatgttaat gagcatggta taacattcca agaatatcgt tttaacgaat ataaaaatca
     7081 aattgaaaaa atcgcttttt acctaggtca tgaaactgtc gcaaagtggt atggtgatta
     7141 tgctgtaaga tacagcataa agaacattaa tactataaga attccaaaag aatccatatc
     7201 cagtgattct tacatcgaaa aagtaaagag tatcactcac cttccagccg cagaatttgt
     7261 aaaaataata atgcgaaaag atgtttggaa tgataatggt tctgtttgtg gttgcagcgg
     7321 taaaggatgt ggcgctgggt gctcatgtaa ttctagataa cataaagccc gtaatatacg
     7381 ggctttaagg attataaatt gaattttatt gaaaagtaca tcatcacatt taataaatgt
     7441 aatttgtttt ttattatatt attatcaact ataagtagtt ttcttttcgt tctattcgga
     7501 tatttaataa aaacagttat tgacaataaa gattcaatag gagaaataaa ctcattatta
     7561 atatttatag catgtttctt atcaattaga ttccttatgc ctgctggata tagcatatcg
     7621 gaatacttga ctcaaaaaac aaatatagaa ttatctgtaa agcttagaga gcaagttatt
     7681 gataatatac aaaattctca ccaagagcat tttttaaaga aaaataaagg agaactaaat
     7741 aaggttatag aaagcatgct ttcttctgca tcatcattat tttatacaat atgttctgat
     7801 gtagtaccat tattaataca aatgattgga ataatcataa caatttgtat aagtgttaat
     7861 acattaatag ccattgagtt tataataata atggcaatct atataatatt tgtcataaaa
     7921 atgacacaac gtagatttc
//
