LOCUS       NZ_CP042575             4921 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain E5 plasmid pE5_004, complete
            sequence.
ACCESSION   NZ_CP042575
VERSION     NZ_CP042575.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12289291
            Assembly: GCF_008931525.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4921)
  AUTHORS   Phan,H.T.T., Stoesser,N., Gordon,K.A., Crook,D., Walker,S.A.,
            Mathers,A.J., Peto,T.E.A., George,S. and Lipworth,S.I.
  TITLE     Genomic Dynamics of Species and Mobile Genetic Elements in a
            Prolonged blaIMP-4-associated Carbapenemase Outbreak in an
            Australian Hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4921)
  AUTHORS   Phan,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2019) Nuffield Department of Micriobiology,
            University of Oxford, Headley Way, Oxford, Oxford OX3 7BN, United
            Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: SEP-2017
            Assembly Method                   :: Unicycler v. SEP-2017
            Assembly Name                     :: E5_oz_imp.uniclycerAssembly.fsa
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: na
            Genome Coverage                   :: 5593x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_008931525.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 20:43:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,080
            CDSs (total)                      :: 4,961
            Genes (coding)                    :: 4,820
            CDSs (with protein)               :: 4,820
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 50 of 141
            Pseudo Genes (incomplete)         :: 102 of 141
            Pseudo Genes (internal stop)      :: 23 of 141
            Pseudo Genes (multiple problems)  :: 29 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP042575.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4921
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="E5"
                     /isolation_source="Shower 3"
                     /db_xref="taxon:158836"
                     /plasmid="pE5_004"
                     /geo_loc_name="Australia: Sydney"
                     /collection_date="2012-06-13"
                     /collected_by="Concord Repatriation Hospital Sydney
                     Australia"
     gene            complement(join(4633..4921,1..35))
                     /locus_tag="FR760_RS25610"
                     /old_locus_tag="FR760_25615"
     CDS             complement(join(4633..4921,1..35))
                     /locus_tag="FR760_RS25610"
                     /old_locus_tag="FR760_25615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_021243036.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARTGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(1042..2328)
                     /locus_tag="FR760_RS25635"
                     /old_locus_tag="FR760_25640"
     CDS             complement(1042..2328)
                     /locus_tag="FR760_RS25635"
                     /old_locus_tag="FR760_25640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002437803.1"
                     /GO_process="GO:0006935 - chemotaxis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting chemotaxis
                     protein"
                     /protein_id="WP_306170312.1"
                     /translation="MFSLNLQRSAISYDAICQNKPVIEFSPEGVINKASPLFLSTMGYR
                     ADEIIGHHHRIFCPPSLVSSPEYSQFWQRLARGESFSGKYLRLAKGNRSVWLEASYIPV
                     SDRRGRVIRVIKIAADISERVHSALEQEAVVNAINRSMAIITFNPEGIVLEANENFVNA
                     TGYKRDEIIGKHHRLFCAETLYKSDEYRHFWESLNQGEFFSGLFPRLNRQGDPLWFRAT
                     YNPVFNSDGQLYKIVKFATDVTADVLRNQREQEAAVHAWDMAVQTRESAQNGANVIENS
                     ILMIDRIAQGMGAVSTDISRLNNQSESIDDMVETIRKFAMQTRLIALNAAIEAARAGAS
                     GRSFAVVAAEVRNLAASVSSATEEIEQVVASNSQLAKDVLCGIENSLMNTREGVTLMRE
                     AGEVMTSIQRNAAGVETAVKDVAISVKAG"
                     /name="PAS domain-containing methyl-accepting chemotaxis
                     protein"
     gene            complement(3242..3457)
                     /locus_tag="FR760_RS25640"
                     /old_locus_tag="FR760_25645"
     CDS             complement(3242..3457)
                     /locus_tag="FR760_RS25640"
                     /old_locus_tag="FR760_25645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021243037.1"
                     /translation="MTELEKQLLSVLEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3464..3955)
                     /locus_tag="FR760_RS25645"
                     /old_locus_tag="FR760_25650"
     CDS             complement(3464..3955)
                     /locus_tag="FR760_RS25645"
                     /old_locus_tag="FR760_25650"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_047720453.1"
                     /translation="MSSLLTLAKDLEQQSKAQQKSTGEMLKAAFSEHEQSVRAELNASA
                     RRISDAISAHEQSMSEAMEKNRRSVLLTAGRTWLTILMVSALLIATSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQAEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            complement(<3990..4694)
                     /locus_tag="FR760_RS26230"
                     /old_locus_tag="FR760_25655"
                     /pseudo=""
     CDS             complement(<3990..4694)
                     /locus_tag="FR760_RS26230"
                     /old_locus_tag="FR760_25655"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          complement(149..321)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.552"
                     /coverage="100.0"
                     /evalue="9.27e-76"
     Typing          246..792
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.727"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          complement(3145..4643)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.297"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cggtgctcgt cctccgtcac ccacatggtg agcatcttgt tccgtttctc tgacaaaccg
       61 gcctcctgat aacgccggga cgggcgggaa agcctttccc ggtcggcgcg gggttggaca
      121 agccgcaggc gcgtcaggct tttggcgggt ttcggggcgc agccctgaac cagtcacgta
      181 gcgctagcgg agtgtatact ggcttaatat gttggcacgg atgagggtgt cagtgaagtg
      241 cttcatgtgg caggagaaaa atgcagcagc ggagcgtcag cagaatatgt gatacaggat
      301 atattccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
      361 accggcttac aggcggggcg gaaatttcct gggagatgcc agaaagaagc ttaacaggga
      421 agcgataagg ccacggcgaa gccgtttttc cataggctcc gcccccctga caagcatcac
      481 gaaatctgac gctcaaatca gtggtggcga aacccgacag gactataaag ataccaggcg
      541 tttccccctg gtggctccct cgtgcgctct cctgttcctg cctttcggtt taccggtgtc
      601 attccgctgt tatggccgcg tttgtctcat tccacgcctg acactcagtt ccgggaaggc
      661 agttcgctcc aagctggact gtatgcacga accccccgtt cagtccgacc gctgcgcctt
      721 atccggtaac tatcaacttg agtccaatcc ggaaaaacac gacaaagcgc cactggcagc
      781 agccactggt aacaggttta gaggaggaga gtcttgaagt ttcgggtgcg ataaggctac
      841 actgaaagga cagttttggt gactgtgctc ctccaagcca gttaccgcgg ttcaaaagag
      901 cgtcaactct tgcgaacctt cgaaaaagcc accttgccgg gtggtttttt cgttttcaga
      961 gcaagagatt acgacgcaac ggaaaggatc tcaagaagat catctgtgat gttcgacaga
     1021 tatggggata tgcaccatgg cctatccggc tttaactgaa atggcgacat ccttaaccgc
     1081 agtctcaacc cctgcagcat tcctctgaat gctggtcata acttcacccg cttcgcgcat
     1141 gagtgtaacc ccttcccgag tgttcatcag gctgttttca atgccacaga gaacatcctt
     1201 ggccagttga ctattgctgg ccacgacttg ctcaatttct tcagtcgcgc tactgacgct
     1261 cgccgcaaga ttacgcactt ctgcggcaac aaccgcaaaa cttcttccgg atgcgccagc
     1321 ccttgctgct tcaatcgcgg cattcagtgc aataagtctt gtctgcatgg caaacttccg
     1381 gatggtttcc accatatcgt caatactttc agactgattg ttgagccgtg agatatcggt
     1441 ggagacagcc cccatcccct gcgcaattct gtcaatcata aggatgctat tttcaataac
     1501 attggcaccg ttctgcgcac tttctcgggt ctgcacggcc atatcccatg catgcacagc
     1561 agcctcttgc tctctctggt ttcgcaggac atcagccgta acatctgtag cgaatttcac
     1621 aattttataa agctgcccat cactgttaaa gacagggtta taagttgcac ggaaccacag
     1681 agggtctccc tgacggttaa ggcgtggaaa cagaccagaa aaaaactcac cttgattcag
     1741 actctcccag aaatgtcggt attcatcact tttgtaaagc gtctcggcac agaacagacg
     1801 atgatgctta cctatgattt catcccgctt atagccagtg gcattgacga aattttcatt
     1861 tgcttcaagc acgatccctt caggattgaa ggtgatgatg gccatagaac ggtttatggc
     1921 atttacaact gcttcctgct caagagcaga atgcacgcgc tcagaaatat cggcagcaat
     1981 tttaatgact ctgataacac ggccgcgcct gtcagaaacg gggatataac tggcttcaag
     2041 ccatacggaa cgatttccct ttgccagccg caggtacttg ccactaaagc tttctcccct
     2101 ggcaaggcgc tgccagaact ggctgtattc cggtgaactt accagcgagg gcgggcagaa
     2161 tatacgatgg tggtgaccga ttatttcatc agccctgtac cccatagtgg agagaaaaag
     2221 cgggctggct ttatttatca ccccctccgg actgaactca atgacaggct tattctgaca
     2281 aattgcgtca tacgaaatgg ctgatcgctg gaggttcagg gagaacatat ttttaagtga
     2341 aaacatatga atcagattgc ccggataatg taagtactgc catggttatc ggctactcct
     2401 tatgtagcgt tatatattac tgcgacaggg cgggaaaaag cccgctattc agccatgccg
     2461 gcatgatgtc cggctccgga tttcacgcgt aaaaaacccg ccatctttag cgggctgaac
     2521 aataagaaaa tgttcatatt tacggcaaat ccgccggctc cagcagagaa gctggcgtct
     2581 atcaatcttg gaagaaccag cgcagctccg catgcattaa caggcatcag aaggcaaaaa
     2641 aatggctccc atccgggagc cggaaaacac tcaactgcct ggatcgcagc acatcctttg
     2701 tgaaggataa tttaagtatc ggccggattg ccgcgggcat aattttttcc gtatccgcca
     2761 gacctcggct tttaacataa caccccttgc ccgcaccgca gaaacggggt tggctgcgtt
     2821 tccggtgcca gacgcgagaa agtggcgaca gacatgacga aaaaacacca aaaacgcagc
     2881 cgctggatat tacactgatg catagtcatg cagccagatg catgcagcgt aaagtcacac
     2941 taaaatcagc gcgaacgcct taaaaaccgg caaatgcctc tccggcaact cacacagctc
     3001 catcaccagg taaaaacagt tcagcagggc ttcaataacg ttgagaattt tcatcacttc
     3061 ctccgggata agtcactacc attccggtag tcagcctatt tagcaggaac gcgatgagcc
     3121 cttcttcgtg cgggctgtca cctcatcttc ggaccatcga aactcttctg cttctgtcgg
     3181 agcgtcagct ccagctcgtt ctgacgctga tactccagtg cagccttctc ctcgatggca
     3241 ttcagttccc ggacaagcgg cgcagctgct cgcttaaact cagcacctgc gtgctcaagt
     3301 ccgacacgcg ctcgttcagc accgcgttct cccgttgcat aagaccacac atgctccgcc
     3361 attccgcgaa ggcgctctcc cattcgtcca gcctttgcga gtagtcctgc tgtagctgct
     3421 ctaatacgct cagcaactgc ttttccagct ctgtcatttt ccgctactcc tgtttcaggc
     3481 ggatccatgt cgtcccgtcg taggggatgg cttcggcttg catccccggc ggcaggataa
     3541 gaaacctccg gccgtcgctg ctctcctgat agcgcactcc ccacgtccgg gcggtcattt
     3601 tcgccagggt atcttcctgc tgacgaagat gcgtgtaatt gtcggttatc tgctgcccct
     3661 gccaccacag aatgctcccg ctcgtggcga tcagcagcgc tgacaccatc aggatggtga
     3721 gccatgtcct cccggccgtc agcaacacgc tccgccggtt tttctccatc gcctcgctca
     3781 tactctgctc gtgggcgctg atggcgtcgc tgattctcct cgcgcttgcg ttcagttccg
     3841 ctctgacaga ctgctcgtgc tcgctgaatg cggctttcag catctcgccg gtactcttct
     3901 gctgcgcttt cgattgctgc tctaagtcct tcgccagcgt taaaagactg ctcatagatg
     3961 gctcccttca gtcggatgtt tcgccccccg tcagggtcgg caatgctgat actgcttttc
     4021 gttgtacgca ccacctcaaa acctgcacct tcaagagcct cagtgacgtc ctgacggctt
     4081 ttcagctccc cggacgaggc taagctcagc agtccacggg tgatggcttc tgcggcttcc
     4141 tgcttcgttt tcggcagtcc ggccggcgtg accagcgccc ggcggttttc cggcgcgttc
     4201 ggatcgtgca gccccagtct gccgttcacg atggtctgcc aggcatcgat acgcggccgg
     4261 tcggcgcggt cgtagtacgg ctggagccgc ctgccggtca gcagctcggt attggggatc
     4321 agaaagttca gctccagacg ccccttgtcc tcgtgctcca cccacagaat gctgtactga
     4381 tctttatcaa gtccgggcat cagaacccgc tcgaagctgg ccatcagttt ttcccgctgg
     4441 ccgggaggca gatcggcctc cgcaaacgac aggacgccgg aggtgtattt cttcacgtaa
     4501 ggcgacgcgt caatcagctc ccggacttct tccggcttcc cctgcaggac gctggcgcct
     4561 tcacgctgcc ggtctttacc cagcagatag tccaccggcc cggcgccacc gccgcggcca
     4621 cgtggatgaa atttaacgat catcgtccgt tcctttctca agaacggcgt gccgcagccg
     4681 ctcaagcccg gcatcgatgg ccatcagcgc cgccacaatc tgcaccctgt catggccggt
     4741 gccgccgccg gcgttaacac ggcgggctat ctggttcagg ttattcccca tgccggcaag
     4801 ctggcgcagc agcgccggcg agattgaggg gagctttccg gtgcgcgcag gtttttcgtc
     4861 caggcaggtc tgccgcatcc acgccgccag ctgcctgccg tcacagcgct caagcagccg
     4921 c
//
