LOCUS       NZ_CP042570             3223 bp    DNA     circular CON 04-APR-2024
DEFINITION  Enterobacter hormaechei strain C44 plasmid pC44_004, complete
            sequence.
ACCESSION   NZ_CP042570
VERSION     NZ_CP042570.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12289368
            Assembly: GCF_008931325.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Phan,H.T.T., Stoesser,N., Gordon,K.A., Crook,D., Walker,S.A.,
            Mathers,A.J., Peto,T.E.A., George,S. and Lipworth,S.I.
  TITLE     Genomic Dynamics of Species and Mobile Genetic Elements in a
            Prolonged blaIMP-4-associated Carbapenemase Outbreak in an
            Australian Hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Phan,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2019) Nuffield Department of Micriobiology,
            University of Oxford, Headley Way, Oxford, Oxford OX3 7BN, United
            Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: SEP-2017
            Assembly Method                   :: Unicycler v. SEP-2017
            Assembly Name :: C44_oz_imp.uniclycerAssembly.fsa
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: na
            Genome Coverage                   :: 1438x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_008931325.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 04:26:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,787
            CDSs (total)                      :: 4,672
            Genes (coding)                    :: 4,584
            CDSs (with protein)               :: 4,584
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 26 of 88
            Pseudo Genes (incomplete)         :: 58 of 88
            Pseudo Genes (internal stop)      :: 18 of 88
            Pseudo Genes (multiple problems)  :: 12 of 88
            Pseudo Genes (short protein)      :: 1 of 88
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP042570.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="C44"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pC44_004"
                     /geo_loc_name="Australia: Sydney"
                     /collection_date="2013-04-15"
                     /collected_by="Concord Repatriation Hospital Sydney
                     Australia"
     gene            112..282
                     /locus_tag="FR837_RS24200"
                     /old_locus_tag="FR837_24195"
     CDS             112..282
                     /locus_tag="FR837_RS24200"
                     /old_locus_tag="FR837_24195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004193911.1"
                     /translation="MHEPPVQSDYHARSGNYQLESNPERHDKSPVAVAIGNWVHGAEIV
                     ENAYRVLEVKA"
                     /name="hypothetical protein"
     gene            complement(491..823)
                     /locus_tag="FR837_RS24210"
                     /old_locus_tag="FR837_24205"
     CDS             complement(491..823)
                     /locus_tag="FR837_RS24210"
                     /old_locus_tag="FR837_24205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EmrE family multidrug efflux SMR transporter"
                     /protein_id="WP_001099531.1"
                     /translation="MNTYIFLGGAIVAEVIGTTFMKYSEGFTRLWPSVGTAISYCAAFY
                     LLSQTLENIPTGIAYAIWSGAGIVLISLAGWLVSGQKLDWPAIAGMFLICVGVLVINIF
                     SKSSAH"
                     /name="EmrE family multidrug efflux SMR transporter"
     gene            complement(991..1206)
                     /locus_tag="FR837_RS24215"
                     /old_locus_tag="FR837_24210"
     CDS             complement(991..1206)
                     /locus_tag="FR837_RS24215"
                     /old_locus_tag="FR837_24210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_024195375.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLIQRENA
                     ALNERVTVLSRQVQSLSEQLRRLSKG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1210..1695)
                     /locus_tag="FR837_RS24220"
                     /old_locus_tag="FR837_24215"
     CDS             complement(1210..1695)
                     /locus_tag="FR837_RS24220"
                     /old_locus_tag="FR837_24215"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_025367738.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     ENYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGQFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(<1730..2434)
                     /locus_tag="FR837_RS24650"
                     /old_locus_tag="FR837_24220"
                     /pseudo=""
     CDS             complement(<1730..2434)
                     /locus_tag="FR837_RS24650"
                     /old_locus_tag="FR837_24220"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2373..2696)
                     /locus_tag="FR837_RS24230"
                     /old_locus_tag="FR837_24225"
     CDS             complement(2373..2696)
                     /locus_tag="FR837_RS24230"
                     /old_locus_tag="FR837_24225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_004193914.1"
                     /translation="MLTMWVTEDEHRRLLERCEGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQIVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     ARG             complement(492..823)
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="41.35"
                     /coverage="102.8"
     Typing          complement(2793..2984)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.821"
                     /coverage="89.0"
                     /evalue="7.28e-73"
     Typing          6..451
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="82.159"
                     /coverage="84.0"
                     /evalue="8.55e-101"
     Typing          2964..3218
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="82.443"
                     /coverage="92.0"
                     /evalue="1.4999999999999999e-53"
     Typing          complement(894..2383)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.784"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgcattcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg gtttctcatt
       61 ccacgcctga aactcggttc cgggtaggca gttcgctcca ggatggactg tatgcacgaa
      121 ccccccgttc agtccgacta ccacgcccgt tccggtaact atcaacttga gtccaacccg
      181 gaaagacacg acaaatcgcc agtggcggta gccattggta actgggttca cggagcagag
      241 attgtagaga atgcctacag agttcttgaa gtgaaagcct gagtgcggct acactgaaag
      301 gacagtttag gtatctgcgc tccgtgaaag ccagttacca tcggaacaaa gagttggtag
      361 ctctgaattc cggcaaacga accaccgttg gtagcggtgg tttttttgtt tacagggaaa
      421 aagattacgc gcagaaagaa aggatctcaa ggaaatcctt ttgctttggc caatatgctt
      481 cggtaaaaag ttaatgcgca gaagattttg aaaaaatatt aataaccaaa actccaacgc
      541 aaataaggaa cattcctgca attgcgggcc aatcaagttt ttgaccgcta accaaccaac
      601 cagctaggct tattagtaca attcccgcac cagaccaaat tgcgtaagca ataccggtag
      661 gtatattttc caatgtttgg gaaagcagat aaaaggcagc gcaataactt attgcagttc
      721 ctacagacgg ccacaggcgt gtaaatcctt ctgaatattt cataaaggtg gtgcctatga
      781 cttcagccac aatagcccct cccaggaaaa tatatgtatt cataaaaata cctcatgttg
      841 aaaaatttaa atatctttac ataacaacta attaatagaa cccggcttat catagcgaag
      901 gaccatgatg ctgccactgc gatcgttcca gctccagcgc tttctggtgc tgcaccgccc
      961 gctcctgctg ccggtgagtt tctatcgcgt tcagcctttc gacaaccggc gcagctgctc
     1021 gctcaaactc tgcacctgcc tgctcaagac cgtgacgcgc tcgttcagcg ccgcgttctc
     1081 ccgttgaata agaccagaca tcgtccgcca ttccgcgaag gcgttctccc actcgtccag
     1141 ccttttcgag tagtcctgct gtagctgctc taatgcgctc agcaactgtt tttccagctc
     1201 cgtcatgtgc tatttccccg ccagtatcat ccagctcgag tcctctccga actgtcccgc
     1261 ttccgggttc accctcacgc agcggcgtct ctgctcgccg caggtcgaga gctgtacgcc
     1321 gctgttcctc tctgacagca tggcctgcgt gctcttctgc tcccggatgg tcgtatagtt
     1381 ctcgagtatc tgctgcccct gccaccacag aatgcccgcg ctcgacgcta tcagcagcgc
     1441 ggagaccaga acgatggtca gccacgtctg gctgaccatg cgcagcatcc ctttcgtgcg
     1501 ctggctcatg gctgaggaca gcttccggtc gtggtcgagg atggcggcgc tgattctctt
     1561 ctcgctttcg ctcagttccg ctctgacaga cttctcgtgc tcgctgaatg cggctttcag
     1621 catctcgccg gtagtctgct gctgcgcttt cgatttctgc tctaagtcct tcgccagcgt
     1681 taaaagactg ttcatagatg gctcccttca gtcggatgtt tcgccccccg tccgggtcgg
     1741 caatgctgat gctgcttttt gtggtgcgca ccacctcaaa acctgcgctt tccagcgcct
     1801 cagtgacgtc ctgacgcgtt ttcagctccc cggatgaggc aagggcaagc aggccgctcg
     1861 taatcgcctg ggcggcttcc tgcttcgctt ccggcagcgc ggaaggcgag accagcaccc
     1921 gccggttctc cggcgcgttc gggtcatgca gccccagcct gccgttcact atggtctgcc
     1981 aggcatcgat gcgcggacgg tcggcgcggt cgtaatacgg ctgtagccgc ttgccggtca
     2041 gaagctccgt gttcgggatc aggaagttca gctccagccg ccccttgtcc cggtgctcaa
     2101 cccacagcac gctgtactgg tctttatcga gtccgggcat cagaacccgc tcgaaactcg
     2161 ccatcagctt ttcgcgctgg ccgggcggta aatcctgttc ggcaaaagac agaacgccgg
     2221 aagtgtactt tttggcgtag ggcgaggcgt cgataagctc ccggacttca tccggcttcc
     2281 cctgcagaac gctggcgccg tcgcgctggc gatctttacc cagcagataa tcgaccggcc
     2341 cgccgccgcc gccgcgcccc cgaggatgaa acttaacgat catcatcagc acccttttcc
     2401 agtacggcat gccgcaaccg ctcgagtccg gcatcgatgg ccatcagcgc ggcgacaatc
     2461 tgcacgcggt cgtgtccgct tccaccaccg gcgttaacct ggcgggcaat ctggttaagg
     2521 ttgttcccca tgccggcaag ctgacgaagc agcgccggcg agatcgacgg aagtttgccg
     2581 gcgcgtgcgg gcttctcgtc caggcacgtc tgccgcatcc aggccgcgag ttgcttaccc
     2641 tcgcagcgct ccagcagacg ccggtgctcg tcctcagtca cccacatggt gagcattttg
     2701 ctgcgtttgt ctgccaatcc ggcctcctga taacgccggg acggacggga aatcttttcc
     2761 cggtcggcac ggtgttggac aagccgcaga cgcggcagtg gttttagcgg gtttcggggc
     2821 gcagccctga accagtcacg tagcgctagc ggagtgtata ctggctaaac atgttatgac
     2881 ggagtggtaa tttatgaaag tgcttcatgt gacaggggaa aaatgcggct gccgggcgta
     2941 agtgcaaaat gcaggtggtg atgaaatgac gcctcctcgc tcactcggtc gctacgctcc
     3001 tgccgtgaga ctgcggcggg cgttaccagc tcacagatta cgggataagc aaatgccgct
     3061 cgaagcagga aaggccaaga aaaggatgcg gagttgccgt ttttccatag gctccgcccc
     3121 cctgacgagt atcacgaaaa tgacgctcaa atcagcggtg gcgaaacccg atgcggctta
     3181 aagataccag gcgtttcccc ctggtggctc cctcgtgctc ccc
//
