LOCUS       NZ_CP042569             4938 bp    DNA     circular CON 04-APR-2024
DEFINITION  Enterobacter hormaechei strain C44 plasmid pC44_003, complete
            sequence.
ACCESSION   NZ_CP042569
VERSION     NZ_CP042569.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12289368
            Assembly: GCF_008931325.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Phan,H.T.T., Stoesser,N., Gordon,K.A., Crook,D., Walker,S.A.,
            Mathers,A.J., Peto,T.E.A., George,S. and Lipworth,S.I.
  TITLE     Genomic Dynamics of Species and Mobile Genetic Elements in a
            Prolonged blaIMP-4-associated Carbapenemase Outbreak in an
            Australian Hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   Phan,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2019) Nuffield Department of Micriobiology,
            University of Oxford, Headley Way, Oxford, Oxford OX3 7BN, United
            Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: SEP-2017
            Assembly Method                   :: Unicycler v. SEP-2017
            Assembly Name :: C44_oz_imp.uniclycerAssembly.fsa
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: na
            Genome Coverage                   :: 1438x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_008931325.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 04:26:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,787
            CDSs (total)                      :: 4,672
            Genes (coding)                    :: 4,584
            CDSs (with protein)               :: 4,584
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 26 of 88
            Pseudo Genes (incomplete)         :: 58 of 88
            Pseudo Genes (internal stop)      :: 18 of 88
            Pseudo Genes (multiple problems)  :: 12 of 88
            Pseudo Genes (short protein)      :: 1 of 88
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP042569.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="C44"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pC44_003"
                     /geo_loc_name="Australia: Sydney"
                     /collection_date="2013-04-15"
                     /collected_by="Concord Repatriation Hospital Sydney
                     Australia"
     gene            join(4475..4938,1..823)
                     /locus_tag="FR837_RS24150"
                     /old_locus_tag="FR837_24145"
     CDS             join(4475..4938,1..823)
                     /locus_tag="FR837_RS24150"
                     /old_locus_tag="FR837_24145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007899670.1"
                     /GO_process="GO:0006935 - chemotaxis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting chemotaxis
                     protein"
                     /protein_id="WP_225986332.1"
                     /translation="MFSLNLQRSAISYDAICQNKPVIEFSPEGVINKASPLFLSTMGYR
                     ADEIIGHHHRIFCPPSLVSSPEYSQFWQRLARGESFSGKYLRLAKGNRSVWLEASYIPV
                     SDRRGRVIRVIKIAADISERVHSALEQEAVVNAINRSMAIITFNPEGIVLEANENFVNA
                     TGYKRDEIIGKHHRLFCAETLYKSDEYRHFWESLNQGEFFSGLFPRLNRQGDSLWFRAT
                     YNPVFNSDGQLYKIVKFATDVTADVLRNQREQEAAVHAWDMAVQTRESAQNGANVIENS
                     ILMIDRIAQGMGAVSTDISRLNNQSESIDDMVETIRKFAMQTRLIALNAAIEAARAGAS
                     GRSFAVVAAEVRNLAASVSSATEEIEQVVASNSQLAKDVLCGIENSLMNTREGVTLMRE
                     AGEVMTSIQRNAAGVETAVKDVAISVKAG"
                     /name="PAS domain-containing methyl-accepting chemotaxis
                     protein"
     gene            1832..2155
                     /locus_tag="FR837_RS24175"
                     /old_locus_tag="FR837_24170"
     CDS             1832..2155
                     /locus_tag="FR837_RS24175"
                     /old_locus_tag="FR837_24170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_007899679.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARTAKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGVGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2364..>2798
                     /locus_tag="FR837_RS24585"
                     /pseudo=""
     CDS             2364..>2798
                     /locus_tag="FR837_RS24585"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2833..3324
                     /locus_tag="FR837_RS24185"
                     /old_locus_tag="FR837_24180"
     CDS             2833..3324
                     /locus_tag="FR837_RS24185"
                     /old_locus_tag="FR837_24180"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_016355729.1"
                     /translation="MSSLLTLAKDLEQQSKAQQKSTGEMLKAAFSEHEQSVRAELNASA
                     RRISDAISAHEQSMSEAMEKNRRSVLLTAGRTWLTILMVSALLIATSGSILWWQGRQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESNDGRRFLILPPGMQAEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            3331..3546
                     /locus_tag="FR837_RS24190"
                     /old_locus_tag="FR837_24185"
     CDS             3331..3546
                     /locus_tag="FR837_RS24190"
                     /old_locus_tag="FR837_24185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          1545..1717
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="9.14e-86"
     Typing          complement(1074..1620)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.545"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          1833..2149
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.509"
                     /coverage="99.0"
                     /evalue="2.38e-59"
     Typing          2146..3647
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="83.692"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agcaaatgaa aatttcgtca atgccactgg ctataagcgg gatgaaatca taggtaagca
       61 tcatcgtctg ttctgtgccg agacgcttta caaaagtgat gaataccgac atttctggga
      121 gagtctgaat caaggtgagt ttttttctgg tctgtttcca cgccttaacc gtcagggaga
      181 ctctctgtgg ttccgtgcaa cttataaccc tgtctttaac agtgatgggc agctttataa
      241 aattgtgaaa ttcgctacag atgttacggc tgatgtcctg cgaaaccaga gagagcaaga
      301 ggctgctgtg catgcatggg atatggccgt gcagacccga gaaagtgcgc agaacggtgc
      361 caatgttatt gaaaatagca tccttatgat tgacagaatt gcgcagggga tgggggctgt
      421 ctccaccgat atctcacggc tcaacaatca gtctgaaagt attgacgata tggtggaaac
      481 catccggaag tttgccatgc agacaagact tattgcactg aatgccgcga ttgaagcagc
      541 aagggctggc gcatccggaa gaagttttgc ggttgttgcc gcagaagtgc gtaatcttgc
      601 ggcgagcgtc agtagcgcga ctgaagaaat tgagcaagtc gtggccagca atagtcaact
      661 ggccaaggat gttctctgtg gcattgaaaa cagcctgatg aacactcggg aaggggttac
      721 actcatgcgc gaagcgggtg aagttatgac cagcattcag aggaatgctg caggggttga
      781 gactgcggtt aaggatgtcg ccatttcagt taaagccgga taggccatgg tgcatatccc
      841 catatctgtc gaacatcaca gatgatcttc ttgagatcct ttccgttgcg tcgtaatctc
      901 ttgctctgaa aacgaaaaaa ccacccggca aggtggcttt ttcgaaggtt cgcaagagtt
      961 gacgctcttt tgaaccgcgg taactggctt ggaggagcac agtcaccaaa actgtccttt
     1021 cagtgtagcc ttatcgcacc cgaaacttca agactctcct cctctaaacc tgttaccagt
     1081 ggctgctgcc agtggcgctt tgtcgtgttt ttccggattg gactcaagtt gatagttacc
     1141 ggataaggcg cagcggtcgg actgaacggg gggttcgtgc atacagtcca gcttggagcg
     1201 aactgccttc ccggaactga gtgtcaggcg tggaatgaga caaacgcggc cataacagcg
     1261 gaatgacacc ggtaaaccga aaggcaggaa caggagagcg cacgagggag ccaccagggg
     1321 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca ccactgattt gagcgtcaga
     1381 tttcgtgatg cttgtcaggg gggcggagcc tatggaaaaa cggcttcgcc gcggccttat
     1441 cgcttccctg ttaagcttct ttctggcatc tcccaggaaa tttccgcccc gcctgtaagc
     1501 cggtaccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     1561 atatatcctg tatcgcatat tctgctgacg ctccgctgct gcatttttct cctgccacat
     1621 gaagcacttc actgatattc gcatccgtgc caacatagta agccagtata cactccgcta
     1681 gcgctacgtg actggttcag ggctgcgccc cgaaacccgc taacagccac tgacgcgcct
     1741 gcggcttgtc caaccccgcg ccgaccggga aaggttttcc cgcccgtccc ggggttatca
     1801 ggagactgtt ttgtcagaga aacggaacaa gatgctcacc atgtgggtga cggaggatga
     1861 gcacaggcgg ctacttgagc gctgcgacgg caggcagctg gcggcgtgga tgcggcagac
     1921 ctgcctggac gaaaagccgg ctcgtaccgc aaaacttcct tcgatctcgc cggcgctgct
     1981 gcgccagctt gccggtatgg gcaacaacct gaaccagata gcccgccgtg ttaacgccgg
     2041 cgtcggtacc ggtcatgacc gggtgcagat tgtggcggcg ctgatggcca tcgatgccgg
     2101 gcttgagcgg ctgcggcacg ccgttcttga gaaaggaacg gacgatgatc gttaaatttc
     2161 atccacgcgg tcgcggcggt ggcgccgggc cggtggacta tctgctgggt aaagaccggc
     2221 agcgtgaagg cgccagcgtc ctgcagggga agccggaaga agtccgggag ctgatcgacg
     2281 cgtcgcctta cgtgaagaaa tacacctccg gcgtactgtc gtttgcggag gccgatctgc
     2341 ctcccggcca gcgggaaaaa ctgatggcca gcttcgagcg ggttctgatg cccggacttg
     2401 ataaagatca gtacagcatt ctgtgggtgg agcacgagga caaggggcgt ctggagctga
     2461 actttctgat ccccaatacc gagctgctga ccggcaggcg gctccagccg tactacgacc
     2521 gcgccgaccg gccgcgtatc gatgcctggc agaccatcgt gaacggcaga ctggggctgc
     2581 acgatccgaa cgcgccggaa aaccgccggg cgctggtcac gccggccgga ctgccgaaaa
     2641 cgaagcagga agccgcagaa gccatcaccc gtggactgct gagcttagcc tcgtccgggg
     2701 agctgaaaag ccgtcaggac gtcactgagg ctcttgaagg tgcaggtttt gaggtggtgc
     2761 gtacaacgaa aagcagtatc agcattgccg accctgacgg ggggcgaaac atccgactga
     2821 agggagccat ctatgagcag tcttttaacg ctggcgaagg acttagagca gcaatcgaaa
     2881 gcgcagcaga agagtaccgg cgagatgctg aaagccgcat tcagcgagca cgagcagtct
     2941 gtcagagcgg aactgaacgc aagcgcgagg agaatcagcg acgccatcag cgcccacgag
     3001 cagagtatga gcgaggcgat ggagaaaaac cggcggagcg tgttgctgac ggccgggagg
     3061 acatggctca ccatcctgat ggtgtcagcg ctgctgatcg ccacgagcgg gagcattctg
     3121 tggtggcagg gacggcagat aaccgacaat tacacacatc ttcgtcagca ggaagatacc
     3181 ctggcgaaaa tgaccgcccg gacgtgggga gtacgctatc aggagagcaa cgacggccgg
     3241 aggttcctta tcctgccgcc ggggatgcaa gccgaagcca tcccctacga cgggacgacc
     3301 tggatccgcc tgaaacagga gtagcggaaa atgacagagc tggaaaagca gttgctgagc
     3361 gcattagagc agctacagca ggactactcg caaaggctgg acgaatggga gagcgccttc
     3421 gcggaatggc ggagcatgtg tggacttatg caacgggaga acgcggtgct gaacgagcgc
     3481 gtgtcggact tgagcacgca ggtgctgagt ttaagcgagc agctgcgccg cttgtccggg
     3541 aactgaatgc catcgaggag aaggctgcac tggagtatca gcgtcagaac gagctggagc
     3601 tgacgctccg acagaagcag aagagtttcg atggtccgaa gatgaggtga cagcccgcac
     3661 gaagaagggc tcatcgcgtt cctgctaaat aggctgacta ccggaatggt agtgacttat
     3721 cccggaggaa gtgatgaaaa ttctcaacgt tattgaagcc ctgctgaact gtttttacct
     3781 ggtgatggag ctgtgtgagt tgccggagag gcatttgccg gtttttaagg cgttcgcgct
     3841 gattttagtg tgactttacg ctgcatgcat ctggctgcat gactatgcat cagtgtaata
     3901 tccagcggct gcgtttttgg tgttttttcg tcatgtctgt cgccactttc tcgcgtctgg
     3961 caccggaaac gcagccaacc ccgtttctgc ggtgcgggca aggggtgtta tgttaaaagc
     4021 cgaggtctgg cggatacgga aaaaattatg ccggccgggc ttatgcccgc ggcaatccgg
     4081 ccgatactta aattatcctt cacaaaggat gtgctgcgat ccaggcagtt gagtgttttc
     4141 cggctcccgg atgtgagcca tttttttgcc ttctgatgcc tgttaatgca tgcggagctg
     4201 cgctggttct tccaagattg atagacgcca gcttctctgc tggagccggc ggatttgccg
     4261 taaatatgaa cattttctta ttgttcagcc cgctaaagat ggcgggtttt ttacgcgtga
     4321 aatccggagc cggacatcat gccggcatgg ctgaagagcg ggctttttcc cgccctgtcg
     4381 cagtaatata taacgctaca taaggagtag ccgataacca tggcagtact tacattatcc
     4441 gggcaatctg attcatatgt tttcacttaa aaatatgttc tccctgaacc tccagcgatc
     4501 agccatttcg tatgacgcaa tttgtcagaa taagcctgtc attgagttca gtccggaggg
     4561 ggtgataaat aaagccagcc cgctttttct ctccactatg gggtacaggg ctgatgaaat
     4621 aatcggtcac caccatcgta tattctgccc gccctcgctg gtaagttcac cggaatacag
     4681 ccagttctgg cagcgccttg ccaggggaga aagctttagt ggcaagtacc tgcggctggc
     4741 aaagggaaat cgttccgtat ggcttgaagc cagttatatc cccgtttctg acaggcgcgg
     4801 ccgtgttatc agagtcatta aaattgctgc cgatatttct gagcgcgtgc attctgctct
     4861 tgagcaggaa gcagtcgtaa atgccataaa ccgttctatg gccatcatca ccttcaatcc
     4921 tgaagggatc gtgcttga
//
