LOCUS       NZ_CP042555             2511 bp    DNA     circular CON 22-APR-2024
DEFINITION  Enterobacter hormaechei strain C45 plasmid pC45_004, complete
            sequence.
ACCESSION   NZ_CP042555
VERSION     NZ_CP042555.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12289303
            Assembly: GCF_008931645.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Phan,H.T.T., Stoesser,N., Gordon,K.A., Crook,D., Walker,S.A.,
            Mathers,A.J., Peto,T.E.A., George,S. and Lipworth,S.I.
  TITLE     Genomic Dynamics of Species and Mobile Genetic Elements in a
            Prolonged blaIMP-4-associated Carbapenemase Outbreak in an
            Australian Hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Phan,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2019) Nuffield Department of Micriobiology,
            University of Oxford, Headley Way, Oxford, Oxford OX3 7BN, United
            Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: SEP-2017
            Assembly Method                   :: Unicycler v. SEP-2017
            Assembly Name :: C45_oz_imp.uniclycerAssembly.fsa
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: na
            Genome Coverage                   :: 2580x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_008931645.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 04:17:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,420
            CDSs (total)                      :: 5,306
            Genes (coding)                    :: 5,211
            CDSs (with protein)               :: 5,211
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 29 of 95
            Pseudo Genes (incomplete)         :: 67 of 95
            Pseudo Genes (internal stop)      :: 14 of 95
            Pseudo Genes (multiple problems)  :: 14 of 95
            Pseudo Genes (short protein)      :: 1 of 95
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP042555.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="C45"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pC45_004"
                     /geo_loc_name="Australia: Sydney"
                     /collection_date="2013-04-27"
                     /collected_by="Concord Repatriation Hospital Sydney
                     Australia"
     gene            complement(join(2328..2511,1..44))
                     /locus_tag="FR772_RS27390"
                     /old_locus_tag="FR772_27390"
     CDS             complement(join(2328..2511,1..44))
                     /locus_tag="FR772_RS27390"
                     /old_locus_tag="FR772_27390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            complement(472..615)
                     /locus_tag="FR772_RS28000"
                     /old_locus_tag="FR772_27400"
     CDS             complement(472..615)
                     /locus_tag="FR772_RS28000"
                     /old_locus_tag="FR772_27400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032610644.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHVKTPLAAATGKGYTRLLVQ
                     SS"
                     /name="hypothetical protein"
     gene            complement(1776..1961)
                     /locus_tag="FR772_RS27415"
                     /old_locus_tag="FR772_27415"
     CDS             complement(1776..1961)
                     /locus_tag="FR772_RS27415"
                     /old_locus_tag="FR772_27415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032105038.1"
                     /translation="MTRQESAALNMAKFIRSQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYTMLNAQTGEEDA"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          1650..1729
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.67e-34"
     Typing          complement(451..1011)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.339"
                     /coverage="91.0"
                     /evalue="0.0"
ORIGIN
        1 agcgcaactg ccgctgcgaa cacggaaaca aaaatctttt tcaaagccag ctccttttat
       61 ccatatcagt tttggtcact aaccatctaa ctataggtca ttttttatgt gaaaagaggc
      121 ttgatggtgg gctgcatatc gagttaggtg cgaggtgtta tggttaaagt gtggtgtctg
      181 gcggggaggg ggcagtgcgg gcttatatct gatgtaatcc gaccgataca tataccaaga
      241 tgatttaagt attcctcttt gtgaggttgg atgtctaatt tataaaagga tcttcttgag
      301 atcctttttt ttcgcgcgta atctcttgcc ctgtaaacga aaaaaccacc cggcaaggtg
      361 gtttttcgaa ggttcagcaa accggcaagt ttctgaaccg tggtaacagg gtgtacaaga
      421 ccgctgccac caaatttgtc cttctagtgt agccgcagtc aggcgtcaac ttcaagaact
      481 ctgcaccagt aatcttgtat accccttacc agtggccgct gccagtggcg tttttacgtg
      541 tctttccggg ttggactcaa gacgatagtt accggataag gcgcagcagt cggactgaac
      601 ggggggttcg tgcatacagt ccatcctgga gcgaactgcc ttcccggaac cgagtgtcag
      661 gcgtggaatg agaaaccgcg gccataacag cggagtgaca ccggtaaacc gaaaggcagg
      721 aatgcggggg agcacgaggg agccatcagg gggaaacgcc tggtatcttt aagccgcatc
      781 gggtttcgcc accactgatt tgagcgtcag atttcgtgat gctcgtcagg ggggcggagc
      841 ctatggaaaa acggcttcgg cgcggcctta tgccttcttc gctaagtatc ttcctggcat
      901 cccctagctg gcatccccta ggaaatttct gctccacccg taagcccgta ccgctcgccg
      961 cagccgaacg accgagcgca gcgagtgagt gagcgaggaa gcggaatata tcctgtatta
     1021 cattttctcc tgacgcgttt tctttcattt tctgcgcctg tcttatgtag cagttatgct
     1081 atgcgttact gcggtgttac attttagcca gtatacactc cgctagcgct gagtgactgg
     1141 tccggcgctg cgcccggaac ccgcctgtac cggttcagca gccgttccgg gctgactgca
     1201 atttttttat tttcctgccc gctaccctgt aaacctttct tctgcgttgc cgttaacagg
     1261 aggtaagcca tgtttggttt ttcttccgga gacctgatga ctcttgatga tgagcgacac
     1321 ctgtttgtgt ctgttgtttc gggctggctc cggcgtgccg gggggatgcc ggagccatga
     1381 tgctggatgc ataccggcag attttgtctg aaactgaacc ggccgtaagg acggtcatgc
     1441 tcgaatttct tgaatccgtt cgcattcatt acatttcgtc ctgagcattt tctgtatttc
     1501 tgtccatact taaacgaggc ggccgtcacg gacgatggtc gtcgttgcat gatgctgtta
     1561 tggatgagtg aaagtaatct aatctaactt gacttacctt ccgggcagct ccccggtttt
     1621 tttttgcctg ttcccgggga accactcagg acaggatgtg tatttgtaat accgcgcacg
     1681 cgccggcatc acaaataccc aggtcagggc tgcgccccga caccccgcca tcgcctcacg
     1741 gccgcgcact gaattgcgca cccgttcacc gattttcacg catcttcctc gcctgtctgt
     1801 gcgttcagca tggtgtaaag tgcttcggcc tgatcgtgca ggtgctcaca gcagccggca
     1861 gcctcatcca gatccatctg ttcgagccgc tcgaggagga ggagtgtctg tgagcggata
     1921 aatttggcca tgttgagggc ggcggattcc tgtctggtca ctggtgattt tccccgtctg
     1981 gtgatgattt ttgcgtgaaa agagcatgcc gtcattctgg tgcatagtca tgcatgccgt
     2041 taaaatttaa caggagcgtt tctggcgggt tccggggtgg tttgttgtgg tttttggtca
     2101 tggttccgtc agaaaagcgc tgagtgcgtc tgaggcggtg cgggcaacgg atgttatggt
     2161 aaattcttgg ggggcttgat tataaaaacc gcacttcctc taatgtcttg tgctttttag
     2221 acaaccctgg aagcttttag ctttggtggt gcaatttgct gggattttgg cggagaaccc
     2281 caggtaaaga aaaaggccac attagcggcc tttttcggag aagatgctca gtgcggaatt
     2341 tcggagactt ctttaacgtc ggttttattg atctgctgtt tgacaccatt cgcgtctgta
     2401 tagccaagca ggcctgagtc tgattcttta ggtttgccgt cgctgacaat agtgcgcccg
     2461 tcattggtgt gtatggcata gcttgtgcgc gtgcagccgg tgagggctga a
//
