LOCUS       NZ_CP042554             4760 bp    DNA     circular CON 22-APR-2024
DEFINITION  Enterobacter hormaechei strain C45 plasmid pC45_003, complete
            sequence.
ACCESSION   NZ_CP042554
VERSION     NZ_CP042554.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12289303
            Assembly: GCF_008931645.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Phan,H.T.T., Stoesser,N., Gordon,K.A., Crook,D., Walker,S.A.,
            Mathers,A.J., Peto,T.E.A., George,S. and Lipworth,S.I.
  TITLE     Genomic Dynamics of Species and Mobile Genetic Elements in a
            Prolonged blaIMP-4-associated Carbapenemase Outbreak in an
            Australian Hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Phan,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2019) Nuffield Department of Micriobiology,
            University of Oxford, Headley Way, Oxford, Oxford OX3 7BN, United
            Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: SEP-2017
            Assembly Method                   :: Unicycler v. SEP-2017
            Assembly Name :: C45_oz_imp.uniclycerAssembly.fsa
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: na
            Genome Coverage                   :: 2580x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_008931645.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 04:17:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,420
            CDSs (total)                      :: 5,306
            Genes (coding)                    :: 5,211
            CDSs (with protein)               :: 5,211
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 29 of 95
            Pseudo Genes (incomplete)         :: 67 of 95
            Pseudo Genes (internal stop)      :: 14 of 95
            Pseudo Genes (multiple problems)  :: 14 of 95
            Pseudo Genes (short protein)      :: 1 of 95
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP042554.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="C45"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pC45_003"
                     /geo_loc_name="Australia: Sydney"
                     /collection_date="2013-04-27"
                     /collected_by="Concord Repatriation Hospital Sydney
                     Australia"
     gene            complement(join(4693..4760,1..847))
                     /gene="lpxO"
                     /locus_tag="FR772_RS27350"
                     /old_locus_tag="FR772_27350"
     CDS             complement(join(4693..4760,1..847))
                     /gene="lpxO"
                     /locus_tag="FR772_RS27350"
                     /old_locus_tag="FR772_27350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728425.1"
                     /GO_process="GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A hydroxylase LpxO"
                     /protein_id="WP_023343074.1"
                     /translation="MLSLIIIFVFLISVLYAHSRGKIKQKFSRQLFDHSTFMAPINMFM
                     TGFSKLPSKQAWFPPETFPELQPLIDNWQVIRDEAVNLQGHIKSSEKNNDAGFNTFFKR
                     GWKRFYLKWYGDAHPSAERLCPVTTQLVSSIPCIKAAMFAELPSGSHLGKHRDPYAGSV
                     RYHLGLSTPNDERCFIEVDGVRRSWHDGEVIIFDETFVHWAQNDTEQTRIILFCDIERP
                     MKWRWSQKINRWIGGVLMSAAASPNDNNDRTGAINHLFKYVNAIRDAGQKLKKRNRKLY
                     YLIKNGVISLIFVWLAVSVFRWL"
                     /name="lpxO"
     gene            complement(1118..1333)
                     /locus_tag="FR772_RS27355"
                     /old_locus_tag="FR772_27355"
     CDS             complement(1118..1333)
                     /locus_tag="FR772_RS27355"
                     /old_locus_tag="FR772_27355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_023343075.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSDQLRRLSSG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1337..1822)
                     /locus_tag="FR772_RS27360"
                     /old_locus_tag="FR772_27360"
     CDS             complement(1337..1822)
                     /locus_tag="FR772_RS27360"
                     /old_locus_tag="FR772_27360"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_023343076.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSVLLIASNAAILWWQSQQIL
                     DNYVSIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(<1857..2561)
                     /locus_tag="FR772_RS28070"
                     /old_locus_tag="FR772_27365"
                     /pseudo=""
     CDS             complement(<1857..2561)
                     /locus_tag="FR772_RS28070"
                     /old_locus_tag="FR772_27365"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2500..2823)
                     /locus_tag="FR772_RS27370"
                     /old_locus_tag="FR772_27370"
     CDS             complement(2500..2823)
                     /locus_tag="FR772_RS27370"
                     /old_locus_tag="FR772_27370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001445156.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3467..3577
                     /locus_tag="FR772_RS27995"
     CDS             3467..3577
                     /locus_tag="FR772_RS27995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048246078.1"
                     /translation="MHDPPVQSDYHAHSGNYQLESNPERHDKIAIGGSHW"
                     /name="hypothetical protein"
     gene            complement(3832..4293)
                     /locus_tag="FR772_RS27675"
     CDS             complement(3832..4293)
                     /locus_tag="FR772_RS27675"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_065707752.1"
                     /translation="MVNTYYGYLLGVTLGEWAMGSDVQWQIDNGYLAQVMGDFSTESPS
                     FETPMFGRYFDTFQAAIHVAKAFPGSTLQPSEYEDKTGMLIRHYYVDCHPYYDRLPPEK
                     SDNFQYDEYPSAIHVAACIDQASKELDELLFGGGYGENGEGHASPIILY"
                     /name="hypothetical protein"
     Typing          complement(2925..3111)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.316"
                     /coverage="87.0"
                     /evalue="1.25e-50"
     Typing          3091..3577
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.959"
                     /coverage="93.0"
                     /evalue="3.59e-159"
     Typing          complement(1021..2510)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.182"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aataatatag ttttcgattc ctttttttta gcttctgacc tgcatcacga atagcattaa
       61 catatttaaa taaatgattt atagcaccag tcctgtcatt attatcgttg ggtgatgctg
      121 ccgctgacat tagcacccca cctatccacc tatttatttt ttgtgaccac cgccacttca
      181 tcggcctttc tatatcacag aacaaaataa tacgtgtctg ctcggtatca ttctgagccc
      241 agtgaacaaa agtttcgtca aaaattatca cttcaccatc atgccaactg cggcgtacac
      301 catcaacttc aataaaacac cgttcatcat ttggtgttga taatcctaaa tgataacgaa
      361 ctgaaccggc ataagggtca cgatgctttc ctaaatggct tcctgaaggc aattcagcaa
      421 acatcgctgc tttaatacag ggaatagagc taaccagttg ggttgttact gggcaaagcc
      481 gctccgctga tggatgagca tctccatacc acttgaggta aaagcgcttc caaccgcgct
      541 taaaaaatgt attaaatcct gcatcattat ttttttcaga tgatttaatg tgcccttgca
      601 aattaacagc ttcgtctctt ataacctgcc agttatcaat taatggttga agttcaggaa
      661 atgtttctgg gggaaaccat gcctgctttg acggcagctt cgaaaagcct gtcataaaca
      721 tatttatcgg agccataaat gttgaatgat caaataactg cctggaaaac ttttgtttta
      781 ttttaccgcg agaatgcgca tacaatacac tgataagaaa aacaaaaata ataataagtg
      841 acagcatgtg tatattcctt gttcacgcca ataataaaaa tctcttaaag ataacataat
      901 gaatgcactt aaattataat ctaaatgcta tttatcaata gtataatcga agcttcacca
      961 gaacatatct actacacagg cagagaggat ttcagcagga attatgctga aatgaatcaa
     1021 agggacggac cgtgatatac ccgctgtcgc tgtgatcgtt cgagctccag cgctttctga
     1081 tgccgcgcct cccgcacctg ctgcagctcg acggcattca gccgctcgac aaccggcgca
     1141 gctgatcgct caaactctgc acctgcctgc tcaagcccgt gacgcgctcg ctcagcgccg
     1201 cgttctcccg ttgcataaga ccagacatcg tccgccattc cgcgaaggcg ctctcccact
     1261 cgtccagcct tttcgagtag tcctgctgta gctgctctaa tgcgctcagc aactgttttt
     1321 ccagctctgt catgtgctat ttccccgcca gtatcatcca gctcgaatcc tctccgaacc
     1381 gtcccgcttc cgggttcacc ctcacgcagc ggcgtctctg ctcgccgcag gtcgagagct
     1441 gcacgccgct gttccgctcg gacagcatcg cctgcgtgct cttctgctcc cggatgctca
     1501 cgtaattgtc gagtatctgc tgactctgcc accacagaat ggccgcgttc gaggcgatca
     1561 gcagcacgga gaccaggacg atggtcagcc acgtctggct gaccatgcgc agcatccctt
     1621 tcgtacgctg gctcatggct gaggacagct tccggtcgtg gtcgaggatg gcggcgctga
     1681 ttctcttctc gctttcgctc agttccgctc tgacagactt ctcgtgctcg ctgaatgcgg
     1741 ctttcagcat ctcgccggta ctctgctgct gcgctttcga tttctgctct aagtccttcg
     1801 ccagcgttaa aagactgttc atagatggct cccttcagtc ggatgtttcg ccccccgtcc
     1861 gggtcggcaa tgctgatgct gctttttgtg gtgcgtacca cctcaaaacc tgcgctttcc
     1921 agcgcctcag tgacgtcctg gcgggttttc agctccccgg acgaggccaa ggccagtaaa
     1981 ccccgtgtaa ttgcctgagc ggcttcctgc ttcgtttccg gcagcgcgga cggcgtgacc
     2041 agcgcccgcc ggttttcagg cgcgttcggg tcatgcagcc ccagcctgcc gttcacgatg
     2101 gtctgccagg catcaatgcg cggacggtcg gcgcggtcgt aatacggctg gagacgcttg
     2161 ccagtcagca gctccgtgtt cgggatcagg aagttcagct ccagccgccc cttatcctgg
     2221 tgctcaaccc acagcacgct gtactggtct ttgtcgagtc cgggcatcag aacccgttca
     2281 aagctcgcca tcagcttttc gcgctgcccg ggcggtaaat ccttttcggc aaaggacagg
     2341 acgccggatg tgtacttttt tgcgtagggt gaggcgtcga taagctccct gacttcttcc
     2401 ggcttcccct gcagaacggt ggcgccctcg cgctgtcggt ctttccccag cagataatcc
     2461 accggaccgg cgccaccgcc gcgccctcgc gggtgaaact taacgatcat cgtctgcacc
     2521 cttttccagc acggcatgcc gcagccgctc gagtccggca tctatggcca tcagcgcggc
     2581 gacgacctgc acgcggtcgt gcccgctgcc accaccggcg ttaacctgcc gggcaatctg
     2641 gttaaggttg ttccccatgc cggcaagctg acgaagcagc gccggcgaga tcgagggaag
     2701 cttgccggcg cgggcgggtt tctcgtccag gcacgtctgg cgcatccagg ctgcaagctg
     2761 tttaccgtcg cagcgctcca gcagacgccg gtgctcatcc tcggtcaccc acatcgtgag
     2821 cattttgctg cgtttgtctg ccaatccggc ctcctgataa cgccgggacg ggcgggaaat
     2881 cttttcccgg tcggcgcggt gttggacaag ccgcaggcgc gtcagtggtt ttagcgggtt
     2941 tcggggcgca gccctgaacc agtcacgtag cgctagcgga gtgtatactg gcttaatatg
     3001 gtaaatcgaa gtgggaattc agggaagtgc ttcatgtggc aggtaaaaaa tgcgtctgtg
     3061 gggcgtaagt gcgaaatgct ggggacgatg aaatgacgcc tcctcgctca ctcggtcgct
     3121 acgctcctgc cgtgagactg cggcgggcgt taccggctca cgaatggcgg gataaagaga
     3181 tggcgctcaa atcaggaagg gctgggaaaa ggatgcggcg ttgccgtttt tccataggct
     3241 ccgcccccct gacaagcatc acgaaatatg acgctcaaat cagtggcggc gaaacccgac
     3301 aggactataa agatcccagg cgtttccccc tggtagctcc ctcgtgcgct ctcctgttcc
     3361 tgcctttcgg tttaccggtg tcattccgct gttatgtggc cgcgtttgtc tcattccacg
     3421 cctgacactc agttccgggt aggcagttcg ctccaagctg gactgtatgc acgacccccc
     3481 cgttcagtcc gactaccacg cccattccgg taactatcaa cttgagtcca acccggaaag
     3541 acacgacaaa atcgccattg gcggtagcca ctggtaagca ggatgtgcga gagatgtatc
     3601 gagagttctt gaagtggtgg ccggactacg gctacactgg aaggacagat ttggtgacac
     3661 gtctcgcaca agactgttac cacggttcag aagttcccca acttactgaa ccttcgaaaa
     3721 accacctccc caggtggttt tttcgttttc agagcaagag attacgcgca gagcaaaacg
     3781 atctcaagaa gatcatttta caaattggat aatatccctc cgcccctaac actaatataa
     3841 aattatcggg cttgcatgcc cctcaccatt ttccccatat ccaccaccaa acaatagttc
     3901 gtcgagttct ttgctagctt gatcaataca cgcagccaca tgaatagcag aagggtactc
     3961 atcatactga aaattatcgc ttttttcagg agggagtcga tcataatacg gatgacaatc
     4021 cacataataa tgacgtatta gcatcccagt tttatcttcg tactctgatg gctgcaacgt
     4081 actccctggg aaagcttttg caacatgtat agcagcttga aaggtatcaa aatatcgccc
     4141 aaacattggc gtttcaaagc taggcgactc tgtcgaaaaa tcccccataa cttgcgccaa
     4201 ataaccatta tctatctgcc attgaacatc tgatcccata gcccactctc ccagagttac
     4261 tcctaataaa taaccataat atgtatttac cataacgctt cttggccgca ccccctgaga
     4321 gcccctcagc gcgtttctgc ggcctgaggg ccgtttccgg cacctttccc cggcccaaac
     4381 cctgaaaaac gcccctgata atttctgcgc ggctgcatgg ctatgcacaa gagtgaatgg
     4441 cggccggttt cgtgaaggaa ataccgtgaa agctccgaaa gaaacgctta attgcacagc
     4501 agcacatgaa cgtgatcaga tgaaatctgt ctactgccgc acttcttcac gaaaggcccg
     4561 ctgagcgtca gattcgggcc tttcttacta aaaagatttc aatagaattt tttagcaggc
     4621 gatttttatc tataaccata cattgagcat agaagaccca aatttatgta tgactcaata
     4681 gaaaaaaaca aatcacagcc atctaaacac tgagacagca agccaaacaa aaatcaaaga
     4741 aataactcca tttttaatca
//
