LOCUS       NZ_CP042544             3223 bp    DNA     circular CON 03-APR-2024
DEFINITION  Enterobacter hormaechei strain C4 plasmid pC4_004, complete
            sequence.
ACCESSION   NZ_CP042544
VERSION     NZ_CP042544.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12289289
            Assembly: GCF_008931585.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Phan,H.T.T., Stoesser,N., Gordon,K.A., Crook,D., Walker,S.A.,
            Mathers,A.J., Peto,T.E.A., George,S. and Lipworth,S.I.
  TITLE     Genomic Dynamics of Species and Mobile Genetic Elements in a
            Prolonged blaIMP-4-associated Carbapenemase Outbreak in an
            Australian Hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Phan,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2019) Nuffield Department of Micriobiology,
            University of Oxford, Headley Way, Oxford, Oxford OX3 7BN, United
            Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: SEP-2017
            Assembly Method                   :: Unicycler v. SEP-2017
            Assembly Name                     :: C4_oz_imp.uniclycerAssembly.fsa
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: na
            Genome Coverage                   :: 3051x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_008931585.1-RS_2024_04_03
            Annotation Date                   :: 04/03/2024 04:06:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,799
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,597
            CDSs (with protein)               :: 4,597
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 27 of 87
            Pseudo Genes (incomplete)         :: 56 of 87
            Pseudo Genes (internal stop)      :: 18 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            Pseudo Genes (short protein)      :: 1 of 87
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP042544.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="C4"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pC4_004"
                     /geo_loc_name="Australia: Sydney"
                     /collection_date="2007-06-28"
                     /collected_by="Concord Repatriation Hospital Sydney
                     Australia"
     gene            308..523
                     /locus_tag="FR758_RS24270"
                     /old_locus_tag="FR758_24265"
     CDS             308..523
                     /locus_tag="FR758_RS24270"
                     /old_locus_tag="FR758_24265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_024195375.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLIQRENA
                     ALNERVTVLSRQVQSLSEQLRRLSKG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            691..1023
                     /locus_tag="FR758_RS24275"
                     /old_locus_tag="FR758_24270"
     CDS             691..1023
                     /locus_tag="FR758_RS24275"
                     /old_locus_tag="FR758_24270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EmrE family multidrug efflux SMR transporter"
                     /protein_id="WP_001099531.1"
                     /translation="MNTYIFLGGAIVAEVIGTTFMKYSEGFTRLWPSVGTAISYCAAFY
                     LLSQTLENIPTGIAYAIWSGAGIVLISLAGWLVSGQKLDWPAIAGMFLICVGVLVINIF
                     SKSSAH"
                     /name="EmrE family multidrug efflux SMR transporter"
     gene            complement(1232..1402)
                     /locus_tag="FR758_RS24285"
                     /old_locus_tag="FR758_24280"
     CDS             complement(1232..1402)
                     /locus_tag="FR758_RS24285"
                     /old_locus_tag="FR758_24280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004193911.1"
                     /translation="MHEPPVQSDYHARSGNYQLESNPERHDKSPVAVAIGNWVHGAEIV
                     ENAYRVLEVKA"
                     /name="hypothetical protein"
     gene            2041..2364
                     /locus_tag="FR758_RS24295"
                     /old_locus_tag="FR758_24290"
     CDS             2041..2364
                     /locus_tag="FR758_RS24295"
                     /old_locus_tag="FR758_24290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_004193914.1"
                     /translation="MLTMWVTEDEHRRLLERCEGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQIVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2573..>3007
                     /locus_tag="FR758_RS24675"
                     /pseudo=""
     CDS             2573..>3007
                     /locus_tag="FR758_RS24675"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     ARG             692..1023
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="41.35"
                     /coverage="102.8"
     Typing          1754..1945
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.821"
                     /coverage="89.0"
                     /evalue="7.28e-73"
     Typing          complement(1291..1771)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.867"
                     /coverage="93.0"
                     /evalue="1.73e-132"
     Typing          2..621
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="86.124"
                     /coverage="100.0"
                     /evalue="9.45e-106"
     Typing          2355..3223
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.552"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agccatgagc cagcgcacga aagggatgct gcgcatggtc agccagacgt ggctgaccat
       61 cgttctggtc tccgcgctgc tgatagcgtc gagcgcgggc attctgtggt ggcaggggca
      121 gcagatactc gagaactata cgaccatccg ggagcagaag agcacgcagg ccatgctgtc
      181 agagaggaac agcggcgtac agctctcgac ctgcggcgag cagagacgcc gctgcgtgag
      241 ggtgaacccg gaagcgggac agttcggaga ggactcgagc tggatgatac tggcggggaa
      301 atagcacatg acggagctgg aaaaacagtt gctgagcgca ttagagcagc tacagcagga
      361 ctactcgaaa aggctggacg agtgggagaa cgccttcgcg gaatggcgga cgatgtctgg
      421 tcttattcaa cgggagaacg cggcgctgaa cgagcgcgtc acggtcttga gcaggcaggt
      481 gcagagtttg agcgagcagc tgcgccggtt gtcgaaaggc tgaacgcgat agaaactcac
      541 cggcagcagg agcgggcggt gcagcaccag aaagcgctgg agctggaacg atcgcagtgg
      601 cagcatcatg gtccttcgct atgataagcc gggttctatt aattagttgt tatgtaaaga
      661 tatttaaatt tttcaacatg aggtattttt atgaatacat atattttcct gggaggggct
      721 attgtggctg aagtcatagg caccaccttt atgaaatatt cagaaggatt tacacgcctg
      781 tggccgtctg taggaactgc aataagttat tgcgctgcct tttatctgct ttcccaaaca
      841 ttggaaaata tacctaccgg tattgcttac gcaatttggt ctggtgcggg aattgtacta
      901 ataagcctag ctggttggtt ggttagcggt caaaaacttg attggcccgc aattgcagga
      961 atgttcctta tttgcgttgg agttttggtt attaatattt tttcaaaatc ttctgcgcat
     1021 taacttttta ccgaagcata ttggccaaag caaaaggatt tccttgagat cctttctttc
     1081 tgcgcgtaat ctttttccct gtaaacaaaa aaaccaccgc taccaacggt ggttcgtttg
     1141 ccggaattca gagctaccaa ctctttgttc cgatggtaac tggctttcac ggagcgcaga
     1201 tacctaaact gtcctttcag tgtagccgca ctcaggcttt cacttcaaga actctgtagg
     1261 cattctctac aatctctgct ccgtgaaccc agttaccaat ggctaccgcc actggcgatt
     1321 tgtcgtgtct ttccgggttg gactcaagtt gatagttacc ggaacgggcg tggtagtcgg
     1381 actgaacggg gggttcgtgc atacagtcca tcctggagcg aactgcctac ccggaaccga
     1441 gtttcaggcg tggaatgaga aaccgcggcc ataacagcgg aatgacaccg gtaaaccgaa
     1501 aggcaggaat gcgggggagc acgagggagc caccaggggg aaacgcctgg tatctttaag
     1561 ccgcatcggg tttcgccacc gctgatttga gcgtcatttt cgtgatactc gtcagggggg
     1621 cggagcctat ggaaaaacgg caactccgca tccttttctt ggcctttcct gcttcgagcg
     1681 gcatttgctt atcccgtaat ctgtgagctg gtaacgcccg ccgcagtctc acggcaggag
     1741 cgtagcgacc gagtgagcga ggaggcgtca tttcatcacc acctgcattt tgcacttacg
     1801 cccggcagcc gcatttttcc cctgtcacat gaagcacttt cataaattac cactccgtca
     1861 taacatgttt agccagtata cactccgcta gcgctacgtg actggttcag ggctgcgccc
     1921 cgaaacccgc taaaaccact gccgcgtctg cggcttgtcc aacaccgtgc cgaccgggaa
     1981 aagatttccc gtccgtcccg gcgttatcag gaggccggat tggcagacaa acgcagcaaa
     2041 atgctcacca tgtgggtgac tgaggacgag caccggcgtc tgctggagcg ctgcgagggt
     2101 aagcaactcg cggcctggat gcggcagacg tgcctggacg agaagcccgc acgcgccggc
     2161 aaacttccgt cgatctcgcc ggcgctgctt cgtcagcttg ccggcatggg gaacaacctt
     2221 aaccagattg cccgccaggt taacgccggt ggtggaagcg gacacgaccg cgtgcagatt
     2281 gtcgccgcgc tgatggccat cgatgccgga ctcgagcggt tgcggcatgc cgtactggaa
     2341 aagggtgctg atgatgatcg ttaagtttca tcctcggggg cgcggcggcg gcggcgggcc
     2401 ggtcgattat ctgctgggta aagatcgcca gcgcgacggc gccagcgttc tgcaggggaa
     2461 gccggatgaa gtccgggagc ttatcgacgc ctcgccctac gccaaaaagt acacttccgg
     2521 cgttctgtct tttgccgaac aggatttacc gcccggccag cgcgaaaagc tgatggcgag
     2581 tttcgagcgg gttctgatgc ccggactcga taaagaccag tacagcgtgc tgtgggttga
     2641 gcaccgggac aaggggcggc tggagctgaa cttcctgatc ccgaacacgg agcttctgac
     2701 cggcaagcgg ctacagccgt attacgaccg cgccgaccgt ccgcgcatcg atgcctggca
     2761 gaccatagtg aacggcaggc tggggctgca tgacccgaac gcgccggaga accggcgggt
     2821 gctggtctcg ccttccgcgc tgccggaagc gaagcaggaa gccgcccagg cgattacgag
     2881 cggcctgctt gcccttgcct catccgggga gctgaaaacg cgtcaggacg tcactgaggc
     2941 gctggaaagc gcaggttttg aggtggtgcg caccacaaaa agcagcatca gcattgccga
     3001 cccggacggg gggcgaaaca tccgactgaa gggagccatc tatgaacagt cttttaacgc
     3061 tggcgaagga cttagagcag aaatcgaaag cgcagcagca gactaccggc gagatgctga
     3121 aagccgcatt cagcgagcac gagaagtctg tcagagcgga actgagcgaa agcgagaaga
     3181 gaatcagcgc cgccatcctc gaccacgacc ggaagctgtc ctc
//
