LOCUS       NZ_CP042543             4938 bp    DNA     circular CON 03-APR-2024
DEFINITION  Enterobacter hormaechei strain C4 plasmid pC4_003, complete
            sequence.
ACCESSION   NZ_CP042543
VERSION     NZ_CP042543.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12289289
            Assembly: GCF_008931585.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Phan,H.T.T., Stoesser,N., Gordon,K.A., Crook,D., Walker,S.A.,
            Mathers,A.J., Peto,T.E.A., George,S. and Lipworth,S.I.
  TITLE     Genomic Dynamics of Species and Mobile Genetic Elements in a
            Prolonged blaIMP-4-associated Carbapenemase Outbreak in an
            Australian Hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   Phan,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2019) Nuffield Department of Micriobiology,
            University of Oxford, Headley Way, Oxford, Oxford OX3 7BN, United
            Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: SEP-2017
            Assembly Method                   :: Unicycler v. SEP-2017
            Assembly Name                     :: C4_oz_imp.uniclycerAssembly.fsa
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: na
            Genome Coverage                   :: 3051x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_008931585.1-RS_2024_04_03
            Annotation Date                   :: 04/03/2024 04:06:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,799
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,597
            CDSs (with protein)               :: 4,597
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 27 of 87
            Pseudo Genes (incomplete)         :: 56 of 87
            Pseudo Genes (internal stop)      :: 18 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            Pseudo Genes (short protein)      :: 1 of 87
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP042543.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="C4"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pC4_003"
                     /geo_loc_name="Australia: Sydney"
                     /collection_date="2007-06-28"
                     /collected_by="Concord Repatriation Hospital Sydney
                     Australia"
     gene            487..978
                     /locus_tag="FR758_RS24225"
                     /old_locus_tag="FR758_24220"
     CDS             487..978
                     /locus_tag="FR758_RS24225"
                     /old_locus_tag="FR758_24220"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_016355729.1"
                     /translation="MSSLLTLAKDLEQQSKAQQKSTGEMLKAAFSEHEQSVRAELNASA
                     RRISDAISAHEQSMSEAMEKNRRSVLLTAGRTWLTILMVSALLIATSGSILWWQGRQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESNDGRRFLILPPGMQAEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            985..1200
                     /locus_tag="FR758_RS24230"
                     /old_locus_tag="FR758_24225"
     CDS             985..1200
                     /locus_tag="FR758_RS24230"
                     /old_locus_tag="FR758_24225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            2129..3415
                     /locus_tag="FR758_RS24235"
                     /old_locus_tag="FR758_24230"
     CDS             2129..3415
                     /locus_tag="FR758_RS24235"
                     /old_locus_tag="FR758_24230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007899670.1"
                     /GO_process="GO:0006935 - chemotaxis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting chemotaxis
                     protein"
                     /protein_id="WP_225986332.1"
                     /translation="MFSLNLQRSAISYDAICQNKPVIEFSPEGVINKASPLFLSTMGYR
                     ADEIIGHHHRIFCPPSLVSSPEYSQFWQRLARGESFSGKYLRLAKGNRSVWLEASYIPV
                     SDRRGRVIRVIKIAADISERVHSALEQEAVVNAINRSMAIITFNPEGIVLEANENFVNA
                     TGYKRDEIIGKHHRLFCAETLYKSDEYRHFWESLNQGEFFSGLFPRLNRQGDSLWFRAT
                     YNPVFNSDGQLYKIVKFATDVTADVLRNQREQEAAVHAWDMAVQTRESAQNGANVIENS
                     ILMIDRIAQGMGAVSTDISRLNNQSESIDDMVETIRKFAMQTRLIALNAAIEAARAGAS
                     GRSFAVVAAEVRNLAASVSSATEEIEQVVASNSQLAKDVLCGIENSLMNTREGVTLMRE
                     AGEVMTSIQRNAAGVETAVKDVAISVKAG"
                     /name="PAS domain-containing methyl-accepting chemotaxis
                     protein"
     gene            4424..4747
                     /locus_tag="FR758_RS24260"
                     /old_locus_tag="FR758_24255"
     CDS             4424..4747
                     /locus_tag="FR758_RS24260"
                     /old_locus_tag="FR758_24255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_007899679.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARTAKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGVGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          4137..4309
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="9.14e-86"
     Typing          complement(3666..4212)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.545"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          4..1298
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="87.872"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          4425..4741
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.509"
                     /coverage="99.0"
                     /evalue="1.8700000000000001e-59"
     Typing          4738..4937
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.507"
                     /coverage="97.0"
                     /evalue="1.5199999999999992e-34"
ORIGIN
        1 gccagcggga aaaactgatg gccagcttcg agcgggttct gatgcccgga cttgataaag
       61 atcagtacag cattctgtgg gtggagcacg aggacaaggg gcgtctggag ctgaactttc
      121 tgatccccaa taccgagctg ctgaccggca ggcggctcca gccgtactac gaccgcgccg
      181 accggccgcg tatcgatgcc tggcagacca tcgtgaacgg cagactgggg ctgcacgatc
      241 cgaacgcgcc ggaaaaccgc cgggcgctgg tcacgccggc cggactgccg aaaacgaagc
      301 aggaagccgc agaagccatc acccgtggac tgctgagctt agcctcgtcc ggggagctga
      361 aaagccgtca ggacgtcact gaggctcttg aaggtgcagg ttttgaggtg gtgcgtacaa
      421 cgaaaagcag tatcagcatt gccgaccctg acggggggcg aaacatccga ctgaagggag
      481 ccatctatga gcagtctttt aacgctggcg aaggacttag agcagcaatc gaaagcgcag
      541 cagaagagta ccggcgagat gctgaaagcc gcattcagcg agcacgagca gtctgtcaga
      601 gcggaactga acgcaagcgc gaggagaatc agcgacgcca tcagcgccca cgagcagagt
      661 atgagcgagg cgatggagaa aaaccggcgg agcgtgttgc tgacggccgg gaggacatgg
      721 ctcaccatcc tgatggtgtc agcgctgctg atcgccacga gcgggagcat tctgtggtgg
      781 cagggacggc agataaccga caattacaca catcttcgtc agcaggaaga taccctggcg
      841 aaaatgaccg cccggacgtg gggagtacgc tatcaggaga gcaacgacgg ccggaggttc
      901 cttatcctgc cgccggggat gcaagccgaa gccatcccct acgacgggac gacctggatc
      961 cgcctgaaac aggagtagcg gaaaatgaca gagctggaaa agcagttgct gagcgcatta
     1021 gagcagctac agcaggacta ctcgcaaagg ctggacgaat gggagagcgc cttcgcggaa
     1081 tggcggagca tgtgtggact tatgcaacgg gagaacgcgg tgctgaacga gcgcgtgtcg
     1141 gacttgagca cgcaggtgct gagtttaagc gagcagctgc gccgcttgtc cgggaactga
     1201 atgccatcga ggagaaggct gcactggagt atcagcgtca gaacgagctg gagctgacgc
     1261 tccgacagaa gcagaagagt ttcgatggtc cgaagatgag gtgacagccc gcacgaagaa
     1321 gggctcatcg cgttcctgct aaataggctg actaccggaa tggtagtgac ttatcccgga
     1381 ggaagtgatg aaaattctca acgttattga agccctgctg aactgttttt acctggtgat
     1441 ggagctgtgt gagttgccgg agaggcattt gccggttttt aaggcgttcg cgctgatttt
     1501 agtgtgactt tacgctgcat gcatctggct gcatgactat gcatcagtgt aatatccagc
     1561 ggctgcgttt ttggtgtttt ttcgtcatgt ctgtcgccac tttctcgcgt ctggcaccgg
     1621 aaacgcagcc aaccccgttt ctgcggtgcg ggcaaggggt gttatgttaa aagccgaggt
     1681 ctggcggata cggaaaaaat tatgccggcc gggcttatgc ccgcggcaat ccggccgata
     1741 cttaaattat ccttcacaaa ggatgtgctg cgatccaggc agttgagtgt tttccggctc
     1801 ccggatgtga gccatttttt tgccttctga tgcctgttaa tgcatgcgga gctgcgctgg
     1861 ttcttccaag attgatagac gccagcttct ctgctggagc cggcggattt gccgtaaata
     1921 tgaacatttt cttattgttc agcccgctaa agatggcggg ttttttacgc gtgaaatccg
     1981 gagccggaca tcatgccggc atggctgaag agcgggcttt ttcccgccct gtcgcagtaa
     2041 tatataacgc tacataagga gtagccgata accatggcag tacttacatt atccgggcaa
     2101 tctgattcat atgttttcac ttaaaaatat gttctccctg aacctccagc gatcagccat
     2161 ttcgtatgac gcaatttgtc agaataagcc tgtcattgag ttcagtccgg agggggtgat
     2221 aaataaagcc agcccgcttt ttctctccac tatggggtac agggctgatg aaataatcgg
     2281 tcaccaccat cgtatattct gcccgccctc gctggtaagt tcaccggaat acagccagtt
     2341 ctggcagcgc cttgccaggg gagaaagctt tagtggcaag tacctgcggc tggcaaaggg
     2401 aaatcgttcc gtatggcttg aagccagtta tatccccgtt tctgacaggc gcggccgtgt
     2461 tatcagagtc attaaaattg ctgccgatat ttctgagcgc gtgcattctg ctcttgagca
     2521 ggaagcagtc gtaaatgcca taaaccgttc tatggccatc atcaccttca atcctgaagg
     2581 gatcgtgctt gaagcaaatg aaaatttcgt caatgccact ggctataagc gggatgaaat
     2641 cataggtaag catcatcgtc tgttctgtgc cgagacgctt tacaaaagtg atgaataccg
     2701 acatttctgg gagagtctga atcaaggtga gtttttttct ggtctgtttc cacgccttaa
     2761 ccgtcaggga gactctctgt ggttccgtgc aacttataac cctgtcttta acagtgatgg
     2821 gcagctttat aaaattgtga aattcgctac agatgttacg gctgatgtcc tgcgaaacca
     2881 gagagagcaa gaggctgctg tgcatgcatg ggatatggcc gtgcagaccc gagaaagtgc
     2941 gcagaacggt gccaatgtta ttgaaaatag catccttatg attgacagaa ttgcgcaggg
     3001 gatgggggct gtctccaccg atatctcacg gctcaacaat cagtctgaaa gtattgacga
     3061 tatggtggaa accatccgga agtttgccat gcagacaaga cttattgcac tgaatgccgc
     3121 gattgaagca gcaagggctg gcgcatccgg aagaagtttt gcggttgttg ccgcagaagt
     3181 gcgtaatctt gcggcgagcg tcagtagcgc gactgaagaa attgagcaag tcgtggccag
     3241 caatagtcaa ctggccaagg atgttctctg tggcattgaa aacagcctga tgaacactcg
     3301 ggaaggggtt acactcatgc gcgaagcggg tgaagttatg accagcattc agaggaatgc
     3361 tgcaggggtt gagactgcgg ttaaggatgt cgccatttca gttaaagccg gataggccat
     3421 ggtgcatatc cccatatctg tcgaacatca cagatgatct tcttgagatc ctttccgttg
     3481 cgtcgtaatc tcttgctctg aaaacgaaaa aaccacccgg caaggtggct ttttcgaagg
     3541 ttcgcaagag ttgacgctct tttgaaccgc ggtaactggc ttggaggagc acagtcacca
     3601 aaactgtcct ttcagtgtag ccttatcgca cccgaaactt caagactctc ctcctctaaa
     3661 cctgttacca gtggctgctg ccagtggcgc tttgtcgtgt ttttccggat tggactcaag
     3721 ttgatagtta ccggataagg cgcagcggtc ggactgaacg gggggttcgt gcatacagtc
     3781 cagcttggag cgaactgcct tcccggaact gagtgtcagg cgtggaatga gacaaacgcg
     3841 gccataacag cggaatgaca ccggtaaacc gaaaggcagg aacaggagag cgcacgaggg
     3901 agccaccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc caccactgat
     3961 ttgagcgtca gatttcgtga tgcttgtcag gggggcggag cctatggaaa aacggcttcg
     4021 ccgcggcctt atcgcttccc tgttaagctt ctttctggca tctcccagga aatttccgcc
     4081 ccgcctgtaa gccggtaccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     4141 cgaggaagcg gaatatatcc tgtatcgcat attctgctga cgctccgctg ctgcattttt
     4201 ctcctgccac atgaagcact tcactgatat tcgcatccgt gccaacatag taagccagta
     4261 tacactccgc tagcgctacg tgactggttc agggctgcgc cccgaaaccc gctaacagcc
     4321 actgacgcgc ctgcggcttg tccaaccccg cgccgaccgg gaaaggtttt cccgcccgtc
     4381 ccggggttat caggagactg ttttgtcaga gaaacggaac aagatgctca ccatgtgggt
     4441 gacggaggat gagcacaggc ggctacttga gcgctgcgac ggcaggcagc tggcggcgtg
     4501 gatgcggcag acctgcctgg acgaaaagcc ggctcgtacc gcaaaacttc cttcgatctc
     4561 gccggcgctg ctgcgccagc ttgccggtat gggcaacaac ctgaaccaga tagcccgccg
     4621 tgttaacgcc ggcgtcggta ccggtcatga ccgggtgcag attgtggcgg cgctgatggc
     4681 catcgatgcc gggcttgagc ggctgcggca cgccgttctt gagaaaggaa cggacgatga
     4741 tcgttaaatt tcatccacgc ggtcgcggcg gtggcgccgg gccggtggac tatctgctgg
     4801 gtaaagaccg gcagcgtgaa ggcgccagcg tcctgcaggg gaagccggaa gaagtccggg
     4861 agctgatcga cgcgtcgcct tacgtgaaga aatacacctc cggcgtactg tcgtttgcgg
     4921 aggccgatct gcctcccg
//
