LOCUS       NZ_CP042516             2694 bp    DNA     circular CON 16-NOV-2023
DEFINITION  Serratia marcescens strain E28 plasmid pE28_004, complete sequence.
ACCESSION   NZ_CP042516
VERSION     NZ_CP042516.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12289419
            Assembly: GCF_008931425.1
KEYWORDS    RefSeq.
SOURCE      Serratia marcescens
  ORGANISM  Serratia marcescens
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Yersiniaceae; Serratia.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Phan,H.T.T., Stoesser,N., Gordon,K.A., Crook,D., Walker,S.A.,
            Mathers,A.J., Peto,T.E.A., George,S. and Lipworth,S.I.
  TITLE     Genomic Dynamics of Species and Mobile Genetic Elements in a
            Prolonged blaIMP-4-associated Carbapenemase Outbreak in an
            Australian Hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Phan,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2019) Nuffield Department of Micriobiology,
            University of Oxford, Headley Way, Oxford, Oxford OX3 7BN, United
            Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: SEP-2017
            Assembly Method                   :: Unicycler v. SEP-2017
            Assembly Name :: E28_oz_imp.uniclycerAssembly.fsa
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: na
            Genome Coverage                   :: 2620x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/16/2023 02:51:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,649
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,420
            CDSs (with protein)               :: 5,420
            Genes (RNA)                       :: 134
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 94
            ncRNAs                            :: 18
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 35 of 96
            Pseudo Genes (incomplete)         :: 66 of 96
            Pseudo Genes (internal stop)      :: 12 of 96
            Pseudo Genes (multiple problems)  :: 16 of 96
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP042516.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Serratia marcescens"
                     /mol_type="genomic DNA"
                     /strain="E28"
                     /isolation_source="Ensuite 7/8"
                     /db_xref="taxon:615"
                     /plasmid="pE28_004"
                     /geo_loc_name="Australia: Sydney"
                     /collection_date="2012-10-12"
                     /collected_by="Concord Repatriation Hospital Sydney
                     Australia"
     gene            complement(join(2306..2694,1..217))
                     /locus_tag="FR888_RS28375"
                     /old_locus_tag="FR888_28370"
     CDS             complement(join(2306..2694,1..217))
                     /locus_tag="FR888_RS28375"
                     /old_locus_tag="FR888_28370"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_047359292.1"
                     /translation="MSFIKSLNKKNQTDQIEVTINVRLTQEEDHLLTLATQQSGYKRNE
                     LFYLLAKEYIFDDESLKEMAEEPGAEFSEGLYQNCYVVNTNRVHHEEDDKFMISEGVAA
                     TFEDGYKEKMEQVKKGDLVFLYRSGEGIVAHGIASGDVLKKDHNGVPDKTYYQKLSGFV
                     RYEKPLTASEIQRILGRNVVFMHTLFSIRDGYKLLKKD"
                     /name="hypothetical protein"
     gene            468..1436
                     /gene="mobV"
                     /locus_tag="FR888_RS28380"
                     /old_locus_tag="FR888_28375"
     CDS             468..1436
                     /gene="mobV"
                     /locus_tag="FR888_RS28380"
                     /old_locus_tag="FR888_28375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_047359293.1"
                     /translation="MANYAIMRCKKLTGMGSVASALQHCYRERETPNADAERTPENYCS
                     VSQSADEAMGKLRELLPEKRRKDAVLAVEYVMTASPEWWKEATPRQQAEFFAHSEQWLE
                     KKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRSKMRDDQSTYAESVK
                     KLGLERGIEGSRATHQTVQHYYQSINRGTRSQVSISPDALEPRVLRKGIFTKDVEDQAA
                     IAKRLSHAVNDGLAGTIAMASQSAQNAKRVRELQKTMDSQQKRLQSVTEPFKGLSREQM
                     TEILMMAQRFKQQNQEKEKQQRIEREKQRQTRSRGMGGMSR"
                     /name="mobV"
     gene            complement(1459..1890)
                     /locus_tag="FR888_RS28385"
                     /old_locus_tag="FR888_28380"
     CDS             complement(1459..1890)
                     /locus_tag="FR888_RS28385"
                     /old_locus_tag="FR888_28380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_080490715.1"
                     /translation="MRRITQVDQTTGEELGGFVAVIRPKQKSSFQRHFTMNQAALITIA
                     NELNHDQMRVLMALLAELDYENYIQVAQMDIAEALTMQKTNVSRAVKNLIDFGIILEGP
                     KIGRSKTYRLNPQFGWKGTVSNHKKALKNGLSVIQGGRS"
                     /name="helix-turn-helix domain-containing protein"
     Typing          421..1433
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaaactcagc gccaggctcc tcagccattt cctttaagct ttcatcatca aagatgtatt
       61 cctttgctag gaggtagaat agctcgttcc ttttataacc agattgctgg gttgctaatg
      121 tcagtaaatg gtcctcttct tgggttaacc taacgttaat cgtcacttct atttggtctg
      181 tttgattttt tttgtttagc gattttatga atgacatctt actttcctca ccagagttaa
      241 tgtactaatc aatgatagtg aatcactata ttgaaacaga agcattaatc acatctttga
      301 tttaagcaat gactttatgt catatatttt ttattaagtt gaatttaacg aacgattttg
      361 cgtagcaaag tctagtgagt acactatcat cctgatagtg gcccgccgga ggcatcgcaa
      421 tgcgatcccc cgactggctg gatgtacgcc agaacgaggg ggtaagcatg gcgaattacg
      481 cgataatgcg ctgcaaaaag ctgacgggaa tgggcagtgt ggccagcgct ttgcagcact
      541 gctacaggga gcgtgagacg ccgaatgctg acgctgaacg gacaccagaa aactactgct
      601 ctgtgtctca gtcggcagat gaggcgatgg gaaagctgcg ggagctgttg ccggaaaaac
      661 gccgtaaaga tgcggtactg gctgttgagt acgttatgac ggcaagccct gagtggtgga
      721 aagaagcgac accgcggcag caggcggagt ttttcgccca ttctgagcag tggctggaga
      781 agaaatacgg gaaggatcgt gttgtggctg cggtagttca ccgggacgag gccaccccgc
      841 atttgtcagc gttcgtggtg ccattgacgc aggatggacg gctgtccgcg aaagagttta
      901 ttggcgggcg gtcaaagatg cgtgacgatc agagcaccta cgctgaatca gtaaaaaaat
      961 tgggtcttga gcgcggcatc gagggaagcc gtgccacgca tcagacagtt cagcactact
     1021 accaatcgat aaaccgtggc acccgtagcc aggtatcaat ctcgccagat gcgctggagc
     1081 cgcgcgtatt gcggaaagga attttcacca aagacgtgga agatcaggca gccatagcca
     1141 aacgtctaag tcacgcagtg aacgatggcc ttgcaggaac catcgccatg gcctctcaga
     1201 gcgcgcagaa tgcaaaaaga gtgcgcgagc tacagaagac tatggactcg cagcagaaac
     1261 gcctacagag cgttacagag ccttttaagg gcctttccag agagcagatg accgaaatcc
     1321 tgatgatggc tcagcggttt aagcagcaaa accaggaaaa ggaaaaacag cagcgtattg
     1381 aacgtgaaaa acagcgacag acgcgatcgc gtggcatggg tggtatgagt cgttagtttt
     1441 gatgcgacat tccagagatc agcttctacc gccctgtata acgctgaggc cgttttttag
     1501 tgcttttttg tggttgctga cagtaccttt ccaaccgaac tggggattca agcgataagt
     1561 tttgctccgt cctatcttag gaccttccag tatgatcccg aaatcaatca ggttttttac
     1621 cgcacgactg acatttgttt tttgcatagt cagcgcttcc gcaatatcca tctgcgcaac
     1681 ttggatgtag ttctcgtaat ccagttctgc gagcaaggcc atcagcacac gcatctggtc
     1741 atggtttagc tcattggcaa tggtaattag cgcggcttgg ttcatagtaa agtgcctctg
     1801 gaatgatgac ttttgtttag gacgaatcac tgctacaaaa ccacctaatt cctcccctgt
     1861 agtttggtct acctgcgtga ttctccgcat ctaaaaatct ccattcagga aaaaatagtt
     1921 atctctcaag ataactaagt tatctctaga gataactatt gtcaaaaact ttccgcttgt
     1981 ggcgcggctt cccagccatc cccttctgta tcttattact ctccacaatc acacatcggc
     2041 aaaatcaggt gcttttatgc ctgttctgag tgttagggtt cctatgtaag tgacgtggaa
     2101 attccgcttc ctcgctcatt cgctcactac gttcggtcat gagtctgcgg cgagcggttc
     2161 ttgatttttt aaaacgatat tcggagcttt atttcgtcaa gggcgagcgt agcgagttca
     2221 tttacccttg acgatgcact gtaaaaaaca cttccggaag tgtggttggt gtcacaaagt
     2281 gatgccaacc acctgtagac tgggtttaat cttttttaag cagtttatag ccatctctaa
     2341 tgctaaatag cgtgtgcata aacacaacgt ttctccccag tattctctgg atctcagacg
     2401 cggttagtgg tttttcgtac ctaacaaagc ctgatagctt ctggtagtag gtcttgtctg
     2461 gcacgccatt atgatctttc tttaaaacat caccactggc gataccatgt gcaacaattc
     2521 cttcgccgga gcgataaaga aagactaaat ctcctttttt aacctgctcc attttttcct
     2581 tataaccatc ttcaaatgtt gccgctacgc cctctgaaat catgaactta tcatcttctt
     2641 catgatgaac tctgttagtg ttaaccacat aacaattttg gtataagcct tctg
//
