LOCUS       NZ_CP042497             8836 bp    DNA     linear   CON 26-JAN-2024
DEFINITION  Leclercia adecarboxylata strain E61 plasmid pE61_004.
ACCESSION   NZ_CP042497
VERSION     NZ_CP042497.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12289350
            Assembly: GCF_008931385.1
KEYWORDS    RefSeq.
SOURCE      Leclercia adecarboxylata
  ORGANISM  Leclercia adecarboxylata
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Leclercia.
REFERENCE   1  (bases 1 to 8836)
  AUTHORS   Phan,H.T.T., Stoesser,N., Gordon,K.A., Crook,D., Walker,S.A.,
            Mathers,A.J., Peto,T.E.A., George,S. and Lipworth,S.I.
  TITLE     Genomic Dynamics of Species and Mobile Genetic Elements in a
            Prolonged blaIMP-4-associated Carbapenemase Outbreak in an
            Australian Hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8836)
  AUTHORS   Phan,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2019) Nuffield Department of Micriobiology,
            University of Oxford, Headley Way, Oxford, Oxford OX3 7BN, United
            Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: SEP-2017
            Assembly Method                   :: Unicycler v. SEP-2017
            Assembly Name :: E61_oz_imp.uniclycerAssembly.fsa
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: na
            Genome Coverage                   :: 2671x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 14:25:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,495
            CDSs (total)                      :: 5,364
            Genes (coding)                    :: 5,236
            CDSs (with protein)               :: 5,236
            Genes (RNA)                       :: 131
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 21
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 49 of 128
            Pseudo Genes (incomplete)         :: 95 of 128
            Pseudo Genes (internal stop)      :: 31 of 128
            Pseudo Genes (multiple problems)  :: 37 of 128
            Pseudo Genes (short protein)      :: 1 of 128
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP042497.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..8836
                     /organism="Leclercia adecarboxylata"
                     /mol_type="genomic DNA"
                     /strain="E61"
                     /isolation_source="Shower 2"
                     /db_xref="taxon:83655"
                     /plasmid="pE61_004"
                     /geo_loc_name="Australia: Sydney"
                     /collection_date="2014-04-07"
                     /collected_by="Concord Repatriation Hospital Sydney
                     Australia"
     gene            137..460
                     /locus_tag="FR819_RS27740"
                     /old_locus_tag="FR819_27740"
     CDS             137..460
                     /locus_tag="FR819_RS27740"
                     /old_locus_tag="FR819_27740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_182481769.1"
                     /translation="MLTMWVTEGEHRRLLERCDGRSLAAWMRQTCLDEKPARTGKLPSL
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            669..>1103
                     /locus_tag="FR819_RS28490"
                     /pseudo=""
     CDS             669..>1103
                     /locus_tag="FR819_RS28490"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1138..1629
                     /locus_tag="FR819_RS27750"
                     /old_locus_tag="FR819_27750"
     CDS             1138..1629
                     /locus_tag="FR819_RS27750"
                     /old_locus_tag="FR819_27750"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032629998.1"
                     /translation="MSSLLTLAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWFTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            1636..1851
                     /locus_tag="FR819_RS27755"
                     /old_locus_tag="FR819_27755"
     CDS             1636..1851
                     /locus_tag="FR819_RS27755"
                     /old_locus_tag="FR819_27755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2522..2764)
                     /locus_tag="FR819_RS27760"
                     /old_locus_tag="FR819_27760"
     CDS             complement(2522..2764)
                     /locus_tag="FR819_RS27760"
                     /old_locus_tag="FR819_27760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023316026.1"
                     /translation="MALVRSGNKSKIFEADTKFNSVFDTEYKPGETCEYEGIYKCTGCG
                     KEVTIAGGKTIPPQNHHQHTPAQGNIRWQLFVYAQ"
                     /name="hypothetical protein"
     gene            2951..3268
                     /locus_tag="FR819_RS27765"
                     /old_locus_tag="FR819_27765"
     CDS             2951..3268
                     /locus_tag="FR819_RS27765"
                     /old_locus_tag="FR819_27765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_023316025.1"
                     /translation="MQQDHEHDDCRIQFRDDWLYQFYKYGKANRKIPTAISASLGRKLD
                     MLNAADKVGDLRSPPGNRFENLNPPLTGYSSIRVNEKYRLIFKWGENGVEDLYLDPHDY
                     R"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            3471..3827
                     /locus_tag="FR819_RS27770"
                     /old_locus_tag="FR819_27770"
     CDS             3471..3827
                     /locus_tag="FR819_RS27770"
                     /old_locus_tag="FR819_27770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075993.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_023316024.1"
                     /translation="MSQATRKPSTVGDIILYEYLEPSGLKINDLAEMLNVHRNSASALV
                     NNNRKLSTDMAFRLAKAFDTTAKFWLNIQQSVDLWEVTSSPRLQEEIDRVIPAHEIIER
                     KHIVDSSEKKHAIA"
                     /name="HigA family addiction module antitoxin"
     gene            complement(<4147..4269)
                     /locus_tag="FR819_RS28495"
                     /pseudo=""
     CDS             complement(<4147..4269)
                     /locus_tag="FR819_RS28495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(4848..5108)
                     /locus_tag="FR819_RS27785"
                     /old_locus_tag="FR819_27785"
     CDS             complement(4848..5108)
                     /locus_tag="FR819_RS27785"
                     /old_locus_tag="FR819_27785"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_151587140.1"
                     /translation="MNANGFKKFEHDHPYGRYGFRQICRYASSIMAQASPARLSQPETT
                     RHKQVSLIITKSHQDPEEKTKHGLPPVNGNTEEIFTGSGQE"
                     /name="hypothetical protein"
     gene            complement(5440..5607)
                     /locus_tag="FR819_RS28050"
     CDS             complement(5440..5607)
                     /locus_tag="FR819_RS28050"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_154819077.1"
                     /translation="MQLKRKNPARAGKVFENIYMKAAIPAFLSKSVYDKKSAHGATREQ
                     GNYFLWWYWA"
                     /name="hypothetical protein"
     gene            6103..7407
                     /locus_tag="FR819_RS27790"
                     /old_locus_tag="FR819_27790"
     CDS             6103..7407
                     /locus_tag="FR819_RS27790"
                     /old_locus_tag="FR819_27790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007899670.1"
                     /GO_process="GO:0006935 - chemotaxis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting chemotaxis
                     protein"
                     /protein_id="WP_063613192.1"
                     /translation="MRSLKNLFSLNRQRSAISYDAICQNKPVIEFSPEGVIHKASPLFL
                     STMGYRADEIIGHHHRIFCPPSLVSSPEYSHFWQRLARGESFSGKFLRRAKGNRSVWLE
                     ASYIPVTDRRGRVTRVIKIAADISERVHSALEQEAVLNAINRSMAIITFNPEGIVLDAN
                     ENFVNATGYKRDEVIGKHHRLFCAETLYKSDEYRQFWERLNQGEFFSGQFPRINRQGDP
                     LWFRATYNPVFNSDGQLYKIVKFATDVTADVLRNQREQEAAVHAWDMAVQTRGSAQNGA
                     NVIENSILMIDRIAQGMGAVSTDVSRLNNQSESIDGMVETIRKFAMQTRLIALNAAIEA
                     ARAGASGRSFAVVAAEVRNLAASVSSATEEIEQVVASNSQLAKDVLSGIENSLMTTREG
                     VTLMREAGEVMTSIQRNAAGVETAVKDVAISVKAG"
                     /name="PAS domain-containing methyl-accepting chemotaxis
                     protein"
     gene            complement(7631..7777)
                     /locus_tag="FR819_RS28400"
                     /old_locus_tag="FR819_27800"
     CDS             complement(7631..7777)
                     /locus_tag="FR819_RS28400"
                     /old_locus_tag="FR819_27800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063613191.1"
                     /translation="MHEPPVQSDRCALSGNYRLESNPERHDKTPLAAATGKRVYKRLLM
                     QSS"
                     /name="hypothetical protein"
     Typing          4632..4797
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.21"
                     /coverage="98.0"
                     /evalue="1.5699999999999999e-68"
     Typing          5283..5356
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.42"
                     /coverage="96.0"
                     /evalue="1.98e-14"
     Typing          8139..8309
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="94.22"
                     /coverage="98.0"
                     /evalue="5.6599999999999995e-68"
     Typing          complement(4157..4665)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.922"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(7609..8215)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.984"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          138..454
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="4.1599999999999996e-60"
     Typing          451..1943
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttcagggctg cgccccgaaa cccgccaaaa gccactgacg cgcctgcggc ttgtccaacc
       61 ccgcgccgac cgggaaaggc tttcccgccc gtcccggcgt tatcaggagg ccggtttgtc
      121 agagaaacgg aacaagatgc tcaccatgtg ggtgacggag ggcgagcacc ggcggctgct
      181 tgagcgctgc gacggcaggt cgctggcggc gtggatgcgg cagacctgcc tggacgaaaa
      241 gcctgcgcgc accggaaagc tcccctcact ctcgccggcg ctgctgcgcc agcttgccgg
      301 tatgggcaat aacctgaacc agatagcccg ccgtgttaac gccggcggcg gcaccggcca
      361 tgacagggta cagattgtgg cggcgctgat ggccatcgat gccgggcttg agcggctgcg
      421 gcacgccgtt cttgagaaag gaacggacga tgatcgttaa atttcatcca cgcggtcgcg
      481 gcggtggcgc cgggccggtg gactatctgc tgggtaaaga ccggcagcgt gaaggcgcca
      541 gcgtcctgca ggggaagccg gaagaagtcc gggagctgat tgacgcgtcg ccttacgtga
      601 agaaatacac ctccggcgta ctgtcgtttg cggaggccga tctgcctccc ggccagcggg
      661 aaaaactgat ggccagcttc gagcgggttc tgatgcccgg actcgataaa gatcagtaca
      721 gcattctgtg ggtggagcac gcggacaagg ggcgtctgga gctgaacttt ctgatcccca
      781 ataccgaact gctgaccggc aggcggctcc agccgtacta cgaccgcgcc gaccggccgc
      841 gcatcgatgc ctggcagacc atcgtgaacg gcagactggg gctgcatgac ccgaacgcgc
      901 cggaaaaccg ccgggcgctg gtcacgccgt ccggactgcc gaaaacgaag caggaggcca
      961 ctgaagccat cacgcgcgga ctgctgaccc ttgcttcgtc cggggagctg aaaaaccgtc
     1021 aggacgtcac tgaggcgctg gaacggtcag gttttgaggt ggtgcgcacc acgaaaagca
     1081 gtatcagcat tgccgacccg gacggggggc gaaacatccg actgaaggga gccatctatg
     1141 agcagtcttt taacgctggc gaaggactta gagcagcaat cgaaagcgca gcagcagagt
     1201 accggcgaga tgctgaaagc cgcattcagc gagcacgagc agtctgtcag agcggaactg
     1261 agcgcaagcg cgaggagaat cagcgacgcc atcctcgcac acgagcagag tatgagcgag
     1321 gcgatggaga aaaaccggcg gagcgtactg cggactgccg ggcggacatg gttcaccatc
     1381 ctgatggtgt cagcgctgct gatcggtacg agcgggagca tactgtggtg gcaggggcag
     1441 cagataaccg acaattacac gcatcttcgt cagcaggaag ataccctggc gaaaatgact
     1501 gcccggacgt ggggagtgcg ctatcaggag agcagcgacg gccggaggtt tcttatcctg
     1561 ccgcccggga tgcaaaccga agccatcccc tacgacggga cgacatggat ccgcctgaaa
     1621 caggagtagc ggaaaatgac agagctggaa aagcagttgc tgagcgcatt agagcagcta
     1681 cagcaggact actcgcaaag gctggacgaa tgggagagcg ccttcgcgga atggcggagc
     1741 atgtgtgggc ttatgcaacg ggagaacgcg gtgctgaacg agcgcgtgtc ggacttgagc
     1801 acgcaggtgc tgagtttaag cgagcagctg cgccgcttgt ccgggaactg aatgccatcg
     1861 agctccggca gagggagaat gagtatcaga aaacactgga gctggagcgc cagccgcaga
     1921 aaacctaccg cgggccgaca ctttaaacca tccaggcagc tgacaacccg cacgaagagg
     1981 ggctcttatt tctcccgcta aataggttcg tgatcaaaaa ggtcacgact tattcaggag
     2041 cgggaaatga agctgttaaa tctcattgaa tcattgctta actgcctgta tctggcggcg
     2101 gagctgtggg actggctgga gaagcatttg tcggttttta aggcattggc gcggatttta
     2161 gtgtgacttt aacatccatg catctggctg catgactatg caccagtgta atattcagcg
     2221 gctgcgtttc tggtgttttt tcgttatgtc tgtcgccact ttctcgcgtc tggcaccgga
     2281 aacgcagcca accccgtttc agcggtgcgc ccaacggatg ttatggtaaa tcagtatcag
     2341 ctgcagatat aaaaaagccg ccaggctggc ggcttgctgt tattttcgtg gctttcctgc
     2401 atctgagcgt tttcctctga cccttccgtc attgttacgg tcacgaggtt gagttccgcc
     2461 gcctggttta ggaacgacaa cgtgtgggtt gagtggtcga cgatctgcca ttttctatac
     2521 ctcactgagc ataaacaaac aattgccaac gaatgttccc ttgcgcgggg gtatgttggt
     2581 ggtggttttg tggtgggatt gttttcccgc ctgcgatagt tacttcttta ccgcaaccgg
     2641 tacatttata aatcccttca tattcacatg tttctcccgg cttatattca gtgtcaaaaa
     2701 ccgagttaaa cttcgtatct gcttcaaaaa tcttgctttt gtttcctgag cgaacaagag
     2761 ccatgcaacc tccaaattat catttatttt gcacacctgc actgtgcatg agtgcattct
     2821 acagcatagc cattgattgc acaagtgcac tgtgctgttg tgcatttgat atgtttgctg
     2881 tactatatcg gaggtaagaa ggtgatcgaa aaaattgcca agttgcggca aaaattgagg
     2941 taaaagaaaa atgcagcaag atcatgaaca tgatgattgc cgcattcagt ttcgagacga
     3001 ttggctatac cagttttaca aatatggtaa agccaatcga aagattccaa ctgcgattag
     3061 tgcttcatta ggtcgcaagc tggatatgct gaatgctgct gataaagttg gagatttgcg
     3121 gtctcccccg ggaaaccgat ttgagaatct caaccctcca ttgacagggt attcatccat
     3181 acgggtgaat gagaagtacc gcttgatatt taagtggggt gaaaacggag tcgaggattt
     3241 gtatctcgat ccacatgatt accgatagat aacaggtcgt ggctgttatc ttataaaaca
     3301 agtaggtgag gcgttccctc acctacatta aaatgtcatt acaaataatg attaataaaa
     3361 aaaatgacgt gactatattt aagataatta ctcatcgcta cagctggtaa tagacgaaat
     3421 acttatcttc ctcatctaac agagagaagt aaaaaattta ggagtaaagc atgtcccaag
     3481 caactagaaa accatcaact gtaggggata ttattcttta tgaatatctc gaaccaagtg
     3541 gtttaaaaat caatgacttg gctgaaatgt tgaatgtgca tagaaactct gccagtgctt
     3601 tagttaacaa taatagaaag ctgtctaccg atatggcttt taggttggct aaagccttcg
     3661 ataccactgc aaagttctgg ctaaatatac agcagtctgt tgatttatgg gaggttacta
     3721 gtagccctcg attgcaagaa gaaatagaca gagtgattcc cgctcatgaa atcatagaaa
     3781 ggaaacacat tgttgatagt tctgagaaga aacatgctat tgcatgattt tttttaaaaa
     3841 aatataagtt atccctcaaa aagaaggctt ctataactag aagccttctt tttgagggat
     3901 aaaaacgctc atgttaactt tcttcttacg atcttcttga gatccgtgct ctgtacgcgt
     3961 aatctcttgc tctgtaaacg aaaaaaccgc ctggggaggc ggtttttcga aggttaagtc
     4021 agttggggaa ctgcttaacc tggtaactgg cttcagcaga gcgcagatac caaatactgt
     4081 ccttccagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     4141 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgttttgt cgtgtctttc
     4201 cgggttggac tcaagtcgat agttaccgga acaggcgcag cggtcggact gaacgggggg
     4261 ttcgtgcata cagtccagct tggagcgaac tgcctacccg gaaccgagtg tcaggcgtgg
     4321 aatgagacaa acgcggccat aacagcggaa tgacaccggt aaaccgaaag gcaggaacag
     4381 gagagcgcac gagggagcca tcagggggaa acgcctggta tctttatagt cctgtcgggt
     4441 ttcgccacca ctgatttgag cgtcagattc tgtgatgctc gtcagggggg cggagcctat
     4501 ggaaaaacgg ctttgccgcg gccttatcgc ttccccgcta agtttcttcc tggtatccac
     4561 caggaaattt ccgccccatt cgtaagcccg caccgctcgc cgcagccgaa cgaccgagcg
     4621 tagcgagtca gtgagcgagg aagcggaata tatcctgtat cacatattct gctgacgcac
     4681 cggtgccgcc tttttcctcc tgccacatga agcacttcac tgacaccctc gtccgtgtca
     4741 acatagtaag ccagtataca ctccgctagc gctacgtgac tggtccggcg ctgcgccgga
     4801 accccatacc ggttcagcag ccgttccggc ctgactgcat ctttttttca ttcctgcccg
     4861 ctacctgtaa aaatttcttc tgtgttgccg ttaacaggag gtaagccatg tttggttttt
     4921 tcttccggat cctgatgact cttagtgatg atgagcgaca cctgcttgtg tcgcgttgtt
     4981 tcaggctggc tcaggcgtgc cggggatgcc tgagccatga tgctggatgc ataccggcag
     5041 atttgtctga aaccgtaccg gccgtaaggg tggtcatgct cgaatttctt gaatccgttc
     5101 gcattcatta catttcgtcc tggtattttc tgaatttctg tccatactta aacgaggcgg
     5161 ccgtcacgga cggtggtcgt cgttgcatga tgctgttatg ggatgagtga acgtaatcta
     5221 atctaacttg acttaccttc cgacagctcg cccggttttt tcctgttccc ggggaaccac
     5281 cggacaggat gtgcatttgt aataccgcgc cggcatcaca aatacttagg tcagggctgc
     5341 tccccgacac cctgccaacg cttcacgacg ccggattaaa atccgttccc gttcaccgtt
     5401 atctgttcag ataatctttc tcccacgaac agaataggct tatgcccaat accaccataa
     5461 aaagtagttt ccctgctctc ttgttgcccc atgtgcgctt tttttgtcat aaacactctt
     5521 cgataagaat gccggaatgg cggctttcat ataaatattt tcgaatacct tcccggcccg
     5581 tgccgggttt tttcgtttta gctgcatgcc aggctccctg gtatcacgca tccttgtcgg
     5641 tatctaaaaa cgggggttag cgtgttcttt tcggacgaaa ctgcatgcca tgcagttgtg
     5701 tgcatagcca tgcatagcgc taaaatactt ccagtgcgtt tctggcggtt ttccgggtgg
     5761 tttgttactg gttttaccgt tctgccgtca gaaacgcgct gaggacctct gcggcggtgc
     5821 gggcaacgga tgttatggta aattcgtcag gttgttgcat taagtttcat gaacaatgca
     5881 acttaagcgc gtgacgaaaa aattaccgcc tgccctctaa ggccggcgcg gcggcggccg
     5941 ataactgagg tgcctgaaac caggttttaa ctctgtgaca cgttgtcttc cgctccctgc
     6001 ccgggagctt tttttctgcc accggcaggg cgggcatgat ggtatccgcg tcctcgggga
     6061 taaccatggc agtccttaca ttatccgagc aatctgatgt atatgcgttc acttaaaaat
     6121 ctgttctccc tgaaccgcca gcgctcagcc atttcgtatg acgcaatttg tcagaataag
     6181 cctgtcattg aattcagtcc agagggggtg atacataaag ccagcccgct ttttctctcc
     6241 actatggggt acagggctga tgaaataatc ggtcaccacc atcgtatatt ctgcccaccc
     6301 tctctggtga gttcaccgga atacagccat ttctggcagc gccttgcccg gggagaaagc
     6361 tttagtggca agttcctgcg gcgggcaaag ggaaatcgtt ccgtatggct tgaagccagt
     6421 tatatccccg ttactgaccg gcgcggtcgt gtgaccagag tcattaaaat tgctgcagat
     6481 atttctgagc gcgtgcattc tgcgcttgag caggaagcag tcctaaatgc cataaaccgt
     6541 tctatggcca taatcacctt caatcctgaa gggatcgtgc ttgatgcaaa tgaaaatttc
     6601 gtcaatgcca ctggctataa acgggatgaa gtcatcggta agcatcatcg tctgttttgt
     6661 gccgagacgc tttacaaaag tgatgaatac cgacagttct gggagaggct gaatcagggt
     6721 gagttttttt ctggtcagtt tccacgtatt aaccgtcagg gagaccctct gtggttccgt
     6781 gcaacttata accctgtctt taacagtgac gggcagcttt ataaaattgt gaaattcgct
     6841 acagatgtca cggctgatgt cctgcgaaac cagagagagc aggaggctgc tgtgcatgca
     6901 tgggatatgg ccgtgcagac acgaggaagt gcgcagaacg gtgcaaatgt tattgaaaat
     6961 agcatcctta tgattgacag aattgcgcag gggatgggag ctgtctccac cgatgtctcc
     7021 cggctcaaca atcagtctga aagtattgac ggtatggtgg aaaccatccg gaagtttgcc
     7081 atgcaaacaa gacttattgc attgaatgcc gcgattgaag cagcaagggc gggcgcatcc
     7141 ggaagaagtt ttgcggttgt tgccgcagag gtgcgtaatc ttgcggcgag cgtcagtagc
     7201 gcgactgaag aaattgagca agtcgtggcc agcaatagtc aactggctaa agatgttctc
     7261 agtggcattg aaaacagcct gatgaccacc cgggaagggg tcacactcat gcgtgaagcg
     7321 ggtgaagtga tgaccagcat tcagaggaat gctgcaggag ttgagactgc ggttaaggat
     7381 gtcgccattt cagttaaagc cggataggcc atggtgcata tccccatatc tgtcgaacat
     7441 cacagatgat cttcttgaga tcctttccgt tgcgtcgtaa tctcttgctc tgaaaacgaa
     7501 aaaaccaccc ggcaaggtgg tttttcgaag gttcattaaa ccggcaagtt tatgaaccgt
     7561 ggtaacaggg tgtacaagac cgctgccacc aaaatcgtcc ttccagttta gccgtagttg
     7621 ggctgagact tcaagaactc tgcatcagta atctcttgta caccctctta ccagtggctg
     7681 ctgccagtgg cgttttgtcg tgtctttccg ggttggactc aagtcgatag ttaccggata
     7741 aggcgcagcg gtcggactga acggggggtt cgtgcataca gtccagcttg gagcgaactg
     7801 ccttcccgga accgagtgtc aggcgtggaa tgagaaaccg cggccataac agcggaatga
     7861 caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccacca gggggaaacg
     7921 cctggtatct ttatagtcct gtcgggtttc gccaccactg atttgagcgt cagattctgt
     7981 gatgcttgtc aggggggcgg agcctatgga aaaacggctt cgccgcagcc ttctctccgt
     8041 tccgctaagt atcttcctgg catcttctag gaattttcca ccccaccggt aagccggtac
     8101 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggcatatct
     8161 ccgttattac atattctgct gacgcgccgg tgccgccttc tttctcctgc cacatgaagc
     8221 acttcactga catccgcatc cgtgccaaca tattaagcca gtatacactc cgctagcgct
     8281 acgtgactgg tccggcgctg cgcccggaac ccgcctgtac cggttcagca gccgttccgg
     8341 cctgactgca attttttttc attcctgccc gctaccctgt aaaaatttct tctgtgttgc
     8401 cgttaacagg aggtaagcca tgtttggttt ttcttccgga tccctgatga ctcttagtga
     8461 tgatgagcga cacctgcttg tgtcgcgttg tttcgggctg gctcaggcgt gccggggggg
     8521 atgcctgagc catgatgctg gatgcatacc ggcagatttt gtctgaaacc gtaccggccg
     8581 taagggcggt catgctcgaa tttcttgaat ccgttcgcat tcattacatt tcgtcctgag
     8641 tattttctga atttctgtcc atacttaaac gaggcggccg tcacggacgg tggtcgtcgt
     8701 tgcatgatgc tgttatggat gagtgaacgt aatctaatct aacttgactt accttccggg
     8761 cagctcgccc ggtttttttt tttcctgttc ccggggaacc actccggaca ggatgtgcat
     8821 ttgtaatacc gcgcac
//
