LOCUS       NZ_CP042492             2494 bp    DNA     circular CON 04-APR-2024
DEFINITION  Enterobacter hormaechei strain C15 plasmid pC15_004, complete
            sequence.
ACCESSION   NZ_CP042492
VERSION     NZ_CP042492.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12289339
            Assembly: GCF_008931405.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Phan,H.T.T., Stoesser,N., Gordon,K.A., Crook,D., Walker,S.A.,
            Mathers,A.J., Peto,T.E.A., George,S. and Lipworth,S.I.
  TITLE     Genomic Dynamics of Species and Mobile Genetic Elements in a
            Prolonged blaIMP-4-associated Carbapenemase Outbreak in an
            Australian Hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Phan,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2019) Nuffield Department of Micriobiology,
            University of Oxford, Headley Way, Oxford, Oxford OX3 7BN, United
            Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: SEP-2017
            Assembly Method                   :: Unicycler v. SEP-2017
            Assembly Name :: C15_oz_imp.uniclycerAssembly.fsa
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: na
            Genome Coverage                   :: 1043x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_008931405.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 04:33:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,023
            CDSs (total)                      :: 4,904
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 37 of 92
            Pseudo Genes (incomplete)         :: 64 of 92
            Pseudo Genes (internal stop)      :: 20 of 92
            Pseudo Genes (multiple problems)  :: 24 of 92
            Pseudo Genes (short protein)      :: 1 of 92
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP042492.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="C15"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pC15_004"
                     /geo_loc_name="Australia: Sydney"
                     /collection_date="2009-02-23"
                     /collected_by="Concord Repatriation Hospital Sydney
                     Australia"
     gene            987..1214
                     /locus_tag="FR808_RS25420"
                     /old_locus_tag="FR808_25420"
     CDS             987..1214
                     /locus_tag="FR808_RS25420"
                     /old_locus_tag="FR808_25420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            1580..1765
                     /locus_tag="FR808_RS25425"
                     /old_locus_tag="FR808_25425"
     CDS             1580..1765
                     /locus_tag="FR808_RS25425"
                     /old_locus_tag="FR808_25425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_013772937.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2056..2247)
                     /locus_tag="FR808_RS25435"
                     /old_locus_tag="FR808_25435"
     CDS             complement(2056..2247)
                     /locus_tag="FR808_RS25435"
                     /old_locus_tag="FR808_25435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021243041.1"
                     /translation="MTLSDDERHLLVSVVSVWLRRAGGDAGAMMLDAYRQILSETEPAV
                     RTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     Typing          complement(1813..1900)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          44..572
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.598"
                     /coverage="88.0"
                     /evalue="0.0"
ORIGIN
        1 agaaagtgaa agaaaacgcg tcaggagaaa atgtgataca gaatatattc cgcttcctcg
       61 ctcactgact cgctccgctc ggtcgttcgg ctgcggcgag cgggacgggc ttacggatgg
      121 atcagaaatt tcctggggga tgccaggaag atacttaacg aagaaagcat aaggccgcgc
      181 caaagccgtt tttccatagg ctccgccccc ctgacaagca tcacagaatc tgacgctcaa
      241 atcagtggtg gcgaaacccg atgcggctta aagataccag gcgtttcccc ctggtggctc
      301 cctcgtgctc ccccgcattc ctgcctttcg gtttaccggt gtcactccgc tgttatggcc
      361 gcggtttctc attccacgcc tgacactcag ttccgggaag gcagttcgct ccaggatgga
      421 ctgtatgcac gaaccccccg ttcagtccga ccgctgcgcc ttatccggta actatcaact
      481 tgagtccaac ccggaaagac acgactaatc gccattggca acggccattg gcaaacagga
      541 tgtgcgagag atgtctcaag agttcttgaa gtggcggcct tagtgaggct acactgaaag
      601 gacaaatttg gtgacatgtc tcgcacaaga ctgttaccac ggttagggga tctgccagga
      661 ttacctaacc ttcgaaaaac cacctgccag ggtggttttt tcgtttacag ggcaagagat
      721 tacgcgcgga aaaaaaggat ctcaagaaga tcctttataa attagacatc caacctcaca
      781 aagaggaata cttaaatcat cttggtatat gtatcggccg gattacatct catataagcc
      841 tcctccccgc ctgacaccac actttgacca tagcacctcg cgcctaactc gatatacagc
      901 ccaccatcaa gcctcttttc acataaaaaa tgacctatag ttagatggtt agtgaccaaa
      961 actgatatgg ataaaaggag ctggctttga aaaagatttt tgtttcagtg ttcgcagcgg
     1021 cagttgcgct ttcagccctc accggctgca cgcgcacaag ctatgccata cacaccaatg
     1081 acggtcgcac tattgtcagc gacggcaaac ctaaagaatc agactcaggc ctgcttggct
     1141 atacagacgc gaatggtgtc aaacagcaga tcaataaaac cgacgttaaa gaagtctccg
     1201 aaattccgca ctgagcatct tctccgaaaa aggccgctaa tgtggccttt ttctttacct
     1261 gtggttctcc gccaaaatcc cagcaaattg catcaccaaa gctaaaagct ttcagggttg
     1321 tctaaaaagc acaagacata agaggaagtg cggtatttta taatcaagcc cccaagaatt
     1381 taccataaca tccgttgccc gcaccgcctg agacgcgttc agcgcgttcc tgacgaaacc
     1441 atgacaaaaa ccacaacaaa ccaccccgga acccgtcaga aacgcgcctg ttaaatttta
     1501 acggcatgca tgactatgca ccagaatgac gccatgctct tttcacgcaa aaatcatcac
     1561 cagacgggga aaatcaccag tgaccagaca ggaatccgcc gccctcaata tggccaaatt
     1621 tatccgcgca cagacacttc tcctccttga gcggctcgag cagatggatc tggatgaggc
     1681 tgccggctgc tgtgagcacc tgcacgatca ggccgaagcg ctttacgcca tgctgaacgc
     1741 acagataggc gaggaaaatg cgtgaaaatc ggtgaacggg tgcgcaattc agtgcgcggc
     1801 cgtgaggcga tggcggggtg tcggggcgca gccctgacca gggtatttgt gatgccggcg
     1861 cgtgcgcggt attacaaatg cacatcctgt cccggaacgg acaccgggaa acagcaaaaa
     1921 aaaccgggcg gcacgcccgg aactcaatca agttagatta gattactctc actcgtccat
     1981 aacagcatca tggaacgacg accaccgtcc gtgacggccg cctcgtttaa gtatggacag
     2041 aaatacagaa aatgctcagg acgaaatgta atgaatgcga acggattcaa gaaattcgag
     2101 catgacagtc cttacggccg gttcggtttc agacaaaatc tgccggtatg catccagcat
     2161 catggctccg gcatcccctc cggcacgccg tagccagacc gaaacaacgg acacaagcag
     2221 gtgtcgctca tcatcactaa gagtcatcag ggctccggaa gaaaaaccaa acatggctta
     2281 cctactgtta acggcaacgc agaagaaagg tttacagggt agcgggcagg gatgaaaaaa
     2341 aaaaattgca gtcaggccgg aacggctgct gaaccggtac aggcgggttc cgggcgcagc
     2401 gccggaccag tcacggagca ctagcggagt gtatactggc ttaagatgta gcatggcagt
     2461 aacacatagc attaatgcca cataagacag gcgc
//
