LOCUS       NZ_CP042491             4853 bp    DNA     circular CON 04-APR-2024
DEFINITION  Enterobacter hormaechei strain C15 plasmid pC15_003, complete
            sequence.
ACCESSION   NZ_CP042491
VERSION     NZ_CP042491.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12289339
            Assembly: GCF_008931405.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4853)
  AUTHORS   Phan,H.T.T., Stoesser,N., Gordon,K.A., Crook,D., Walker,S.A.,
            Mathers,A.J., Peto,T.E.A., George,S. and Lipworth,S.I.
  TITLE     Genomic Dynamics of Species and Mobile Genetic Elements in a
            Prolonged blaIMP-4-associated Carbapenemase Outbreak in an
            Australian Hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4853)
  AUTHORS   Phan,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2019) Nuffield Department of Micriobiology,
            University of Oxford, Headley Way, Oxford, Oxford OX3 7BN, United
            Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: SEP-2017
            Assembly Method                   :: Unicycler v. SEP-2017
            Assembly Name :: C15_oz_imp.uniclycerAssembly.fsa
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: na
            Genome Coverage                   :: 1043x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_008931405.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 04:33:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,023
            CDSs (total)                      :: 4,904
            Genes (coding)                    :: 4,812
            CDSs (with protein)               :: 4,812
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 37 of 92
            Pseudo Genes (incomplete)         :: 64 of 92
            Pseudo Genes (internal stop)      :: 20 of 92
            Pseudo Genes (multiple problems)  :: 24 of 92
            Pseudo Genes (short protein)      :: 1 of 92
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP042491.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4853
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="C15"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pC15_003"
                     /geo_loc_name="Australia: Sydney"
                     /collection_date="2009-02-23"
                     /collected_by="Concord Repatriation Hospital Sydney
                     Australia"
     gene            join(4672..4853,1..247)
                     /locus_tag="FR808_RS25360"
                     /old_locus_tag="FR808_25360"
     CDS             join(4672..4853,1..247)
                     /locus_tag="FR808_RS25360"
                     /old_locus_tag="FR808_25360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222531.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_032634161.1"
                     /translation="MSYAIEKKWKAGGLSLGWTAVPTVLFFIQNEKKLSPVAFNTLLNL
                     IVHWWNLQEWPHPSMESLAIRMGVSVRTVQRAINDLEKANLLDKKPTSKSDNRYGGRNI
                     YDLTKLVDYLDIIGPSVSEQVRKPRHKKPIYTVRKKAD"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(304..795)
                     /locus_tag="FR808_RS25365"
                     /old_locus_tag="FR808_25365"
     CDS             complement(304..795)
                     /locus_tag="FR808_RS25365"
                     /old_locus_tag="FR808_25365"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_127627441.1"
                     /translation="MVNQPSVDKGSDNTHVGDVVCFLSTHCLMELFMNADEVTRILAQS
                     SQNDWIVDDETGAFTYKNDLALHIKRADYDDYNSFNEPWAVRHPDSNACSVEYTVHYGS
                     SFVDRKTLVSVDGHRATLPMPASATDLNVSSADVNFAKIVNIGDRVDEYIQRSGLTIQP
                     "
                     /name="hypothetical protein"
     gene            complement(1259..1474)
                     /locus_tag="FR808_RS25370"
                     /old_locus_tag="FR808_25370"
     CDS             complement(1259..1474)
                     /locus_tag="FR808_RS25370"
                     /old_locus_tag="FR808_25370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1481..1972)
                     /locus_tag="FR808_RS25375"
                     /old_locus_tag="FR808_25375"
     CDS             complement(1481..1972)
                     /locus_tag="FR808_RS25375"
                     /old_locus_tag="FR808_25375"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015060400.1"
                     /translation="MSSLLTLAKDLEQQSKAQKQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            complement(<2007..2711)
                     /locus_tag="FR808_RS25925"
                     /old_locus_tag="FR808_25380"
                     /pseudo=""
     CDS             complement(<2007..2711)
                     /locus_tag="FR808_RS25925"
                     /old_locus_tag="FR808_25380"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2650..2973)
                     /locus_tag="FR808_RS25385"
                     /old_locus_tag="FR808_25385"
     CDS             complement(2650..2973)
                     /locus_tag="FR808_RS25385"
                     /old_locus_tag="FR808_25385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_015060398.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARSGKLPSL
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(3959..4387)
                     /locus_tag="FR808_RS25405"
                     /old_locus_tag="FR808_25405"
     CDS             complement(3959..4387)
                     /locus_tag="FR808_RS25405"
                     /old_locus_tag="FR808_25405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000534558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032634162.1"
                     /translation="MGSGSSTQNPQLNVVSFSETFNLYYTSRFSQKTLDKIDDFIEHFE
                     KYNLFGDALKGHPAWIGKVSPSWNVPDSYENKEAIEEHARANKLWHAHIGDPVFIDTFH
                     GKYKVSDWVIHFQRLAPNHIKLLELGYHDPMQLPQNFE"
                     /name="hypothetical protein"
     Typing          complement(3089..3262)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.429"
                     /coverage="100.0"
                     /evalue="5.6200000000000006e-73"
     Typing          3186..3732
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.455"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          complement(1162..2660)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.287"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2657..2973)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.34"
                     /coverage="99.0"
                     /evalue="1.6e-61"
ORIGIN
        1 gagcctggct atacggatgg gggtgtcagt aaggacggtg caaagggcta ttaatgacct
       61 agaaaaagcc aatctcttag ataaaaaacc gacatcaaaa agcgacaacc gatatggtgg
      121 ccgcaacata tacgacctta caaagcttgt agattattta gacataattg gtccttctgt
      181 gtccgagcaa gtaagaaagc cgcgacataa aaagccaata tatacagtaa ggaaaaaagc
      241 cgattagtta tgctggttac atgcaggaac agcccgcatt tggaggccgg gcttatcgtt
      301 aaattagggc tgaatcgtaa gaccagaacg ttgaatgtat tcatcaacgc gatcgccaat
      361 gtttacaatt ttggcgaaat tcacatcagc cgatgacacg tttagatccg ttgcggatgc
      421 aggcataggc aaggttgcac gatgaccatc gacggaaacc aaggtttttc tatcaacaaa
      481 cgaagaacca tagtgaacgg tgtactcgac tgaacacgca ttactatcag gatgacgaac
      541 agcccaaggc tcattaaacg agttgtagtc gtcgtaatca gctctcttaa tgtgcagtgc
      601 taaatcattt ttataagtaa acgcccctgt ctcatcatcc acaatccaat cattttgact
      661 cgattgcgca agaattcgtg taacttcatc agcattcata aatagctcca tcaggcagtg
      721 tgtagataaa aagcaaacaa catcacctac atgggtatta tcagaacctt tatcaacgga
      781 cggttgattt accataacat ctgttgcccg cacagctgaa acggggttgg ctgcgtttcc
      841 ggtgccagac gcgagaaagt ggcgacagac atgacgaaaa aacaccggaa acgcagccgc
      901 tggatattac acttatgcat agtcatgcag ccagatgcat gccgcgtaaa gtcacactaa
      961 aatcagcgcg aacgccttaa aaaccggcaa atgcctctcc ggcaactcac acagctccat
     1021 caccaggtaa aaacagttca gcagggcttc aataacgtta agaattttca tcacttcatc
     1081 cgggataagt cactaccatt ccggtagtca gcctatttag caggaacgcg atgagccctt
     1141 cttcgtgcgg gctgtcacct catcttcgga ccatcaaaac tcttctgctt ctgacggagc
     1201 gtcagctcca gctcgttctg acgctgatac tccagtacag ccttctcctc gatggcattc
     1261 agttcccgga caaacggcgc agctgctcgc ttaaactcag cacctgcgtg ctcaagtccg
     1321 acacgcgctc gttcagcacc gcgttctccc gttgcataag cccacacatg ctccgccatt
     1381 ccgcgaaggc gctctcccat tcgtccagcc tttgcgagta gtcctgctgt agctgctcta
     1441 atgcgctcag caactgcttt tccagctctg tcatcttccg ctactcctgt ttcaggcgga
     1501 tccatgtcgt cccgtcgtaa gggatggctt cggtttgcat ccccggcggc aggataagaa
     1561 acctccggcc gtcgctgctc tcctgatagc gcactcccca cgtccgggcg gtcattttcg
     1621 ccagggtatc ttcctgctga cgaagatgcg tgtaattgtc ggttatctgc tgcccctgcc
     1681 accacagaat gctcccgctc gtgccgatca gcagcgctga caccatcagg atggtgagcc
     1741 atgtccgccc ggcagtccgc agtacgctcc gccggttttt ctccatcgcc tcgctcatac
     1801 tctgctcgtg ggcgaggatg gcgtcgctga ttctcctcgc gcttgcgctc agttccgctc
     1861 tgacagactg ctcgtgctcg ctgaatgcgg ctttcagcat ctcgccggta ctctgcttct
     1921 gcgctttcga ttgctgctct aagtccttcg ccagcgttaa aagactgctc atagatggct
     1981 cccttcagtc ggatgtttcg ccccccgtcc gggtcggcaa tgctgatact gcttttcgtg
     2041 gtgcgcacca cctcaaaacc tgaccgttcc agcgcctcag tgacatcctg acggcttttc
     2101 agctccccgg acgaagcaag ggtcagcagt ccacgcgtga tggcttctgc ggcctcctgc
     2161 ttcgttttcg gcagtccgga cggcgtgacc agcgcccggc ggttttccgg cgcgttcggg
     2221 tcatgcagcc ccagtctgcc gttcacgatg gtctgccagg catcgatgcg cggccggtcg
     2281 gcgcggtcgt agtacggctg gagccgcctg ccggtcagca gctcggtatt ggggatcaga
     2341 aagttcagct ccagacgccc cttgtccgcg tgctccaccc acagaatgct gtactgatct
     2401 ttatcaagtc cgggcatcag aacccgctcg aagctggcca tcagtttttc ccgctggccg
     2461 ggaggcagat cggcctccgc aaacgacagg acgccggaag tgtatttctt cacgtaaggc
     2521 gacgcgtcaa tcagctcccg gacttcttcc ggcttcccct gcaggacggt ggcaccatca
     2581 cgctgccggt ctttacccag caggtagtcc accggcccgg cgccaccgcc gcgaccgcgt
     2641 ggatgaaatt taacgatcat cgtccgttcc tttctcaaga acggcgtgcc gcagccgctc
     2701 aagtccggca tcgatggcca tcagcgccgc cacaatctgc accctgtcat ggccagtgcc
     2761 gccgccggcg ttaacgcggc gggctatctg gttcaggtta ttgcccatac cggcaagctg
     2821 gcgcagcagc gccggcgaga gtgaggggag ctttccggaa cgtgccggct tttcgtccag
     2881 gcaggtctgc cgcatccacg ctgccagctg cctgccgtcg cagcgctcaa gcagccgccg
     2941 gtgctcgtcc tccgtcaccc acatggtgag catcttgttc cgtttctctg acaaaccggc
     3001 ctcctgataa cgccgggacg ggcgggaaag cctttcccgg tcggcgcggg gttggacaag
     3061 ccgcaggcgc gtcagtggct tttagcgggt ttcggggcgc agccctgaac cagtcacgca
     3121 gcgctagcgg agtgtatact ggcttactat gttggcacgg gtgcgggaat cagtgaagtg
     3181 cttcatgtgg caggagaaaa aaggcggcac cggcgcgtaa gcagaatatg cgatacagga
     3241 tatattccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     3301 tgcgggctta cgaatggggc ggaaatttcc tggtagatgc caggaagaaa cttaacaggg
     3361 aaacgataag gccgcggcga agccgttttt ccataggctc cgcccccctg acaagcatca
     3421 cgaaatctga cgctcaaatc agtggtggcg aaacccgaca ggacttaaag ataccaggcg
     3481 tttccccctg gtggctccct cgtgcgctct cctgttcctg cctttcggtt taccggtgtc
     3541 attccgctgt tatggccgcg tttgtctcat tccacgcctg acactcagtt ccgggaaggc
     3601 agttcgctcc aagctggact gtatgcacga accccccgtt cagtccgacc gctgcgcctt
     3661 atccggtaac tatcaacttg agtccaatcc ggaaaaacac gacaaagcgc cactggcagc
     3721 agccactggt aacaggttta gaggaggaga gtcttgaagt ttcgggtgcg ataaggctac
     3781 actgaaagga cagttttggt gactgtgctc ctccaagcca gttaccgcgg ttcaaaagag
     3841 cgtcaactct tgcgaacctt cgaaaaagcc accttgccgg gtggtttttt cgttttcacc
     3901 gcaagagatt acgacgaggg tcaacgaatc tcaagggatc attcatccga acaagagatc
     3961 attcgaaatt ttgggggagt tgcatagggt catgataccc aagctctaac agttttatat
     4021 ggttcggcgc tagacgttgg aagtggatga cccaatcaga gactttgtac ttcccgtgaa
     4081 atgtatcgat gaaaacggga tcgcctatat gggcatgcca caatttatta gccctggcat
     4141 gttcttcaat agcttctttg ttttcgtaac tgtcaggcac gttccaagat ggcgatacct
     4201 tgccaatcca agccggatgg ccttttaagg catcgccaaa aagattgtac ttctcgaaat
     4261 gttcgatgaa gtcatcgatt ttatcgagtg ttttttgaga aaagcgggat gtgtaataaa
     4321 gattgaatgt ctcagagaaa gagacaacat tcaattgtgg attttgcgtg cttgatccgc
     4381 tgcccatgct cttctctgct ccctacttag accagatggc attgtaacag atctctcatt
     4441 cgctctggaa atcaacagag acaaggatac accaaatatt tcagtatctt ttacaagatc
     4501 atgatgttgt ttggttttca tatcactaac tccgtattta atgcagatag aaatgttttt
     4561 ctgttctcag attagccttt tatagttgtg taataatggc gctggtgtca ccctattcat
     4621 atgaatactt atattatata tatctcaaaa aaaatattta caggataatc aatgagttac
     4681 gcaatagaaa aaaaatggaa ggctggaggt ctgtcattag gatggactgc agtacctact
     4741 gtgttgtttt ttatacaaaa tgagaagaaa ttgagccctg tagcattcaa taccttgctg
     4801 aatctcatag tacactggtg gaatctacag gagtggccgc acccctcaat gga
//
