LOCUS       NZ_CP042340            12602 bp    DNA     circular CON 21-JUN-2024
DEFINITION  Escherichia coli strain GZ04-0086 plasmid p13k-GZ04, complete
            sequence.
ACCESSION   NZ_CP042340
VERSION     NZ_CP042340.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN11174938
            Assembly: GCF_004405205.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12602)
  AUTHORS   Yang,L.
  TITLE     NDM-5-producing Escherichia coli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12602)
  AUTHORS   Yang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-AUG-2019) Institute of Disease Prevention and Control,
            Academy of Military Medical Sciences, Dongda street 20, Beijing
            100071, China
REFERENCE   3  (bases 1 to 12602)
  AUTHORS   Yang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAR-2019) Institute of Disease Prevention and Control,
            Academy of Military Medical Sciences, Dongda Street 20, Beijing
            100071, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 124.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_004405205.2-RS_2024_06_21
            Annotation Date                   :: 06/21/2024 03:34:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,099
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,705
            CDSs (with protein)               :: 4,705
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 93 of 274
            Pseudo Genes (incomplete)         :: 185 of 274
            Pseudo Genes (internal stop)      :: 56 of 274
            Pseudo Genes (multiple problems)  :: 53 of 274
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP042340.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12602
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="GZ04-0086"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p13k-GZ04"
                     /geo_loc_name="China: Guangzhou"
                     /lat_lon="23.12 N 113.28 E"
                     /collection_date="2018"
                     /collected_by="Lang Yang"
     gene            complement(join(11923..12602,1..549))
                     /locus_tag="E3B71_RS27425"
                     /old_locus_tag="E3B71_25575"
     CDS             complement(join(11923..12602,1..234,234..549))
                     /locus_tag="E3B71_RS27425"
                     /old_locus_tag="E3B71_25575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="WP_088895425.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAASQ
                     LFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVEYG
                     RGKKVDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWMDGRRSRHTDDTDVLLR
                     IHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRIMRQNALLLERKPAVPPSKR
                     AHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWAVTTGGFNSETVQ
                     DVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGI
                     AESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQRACN
                     GLSDNRCLEI"
                     /name="IS3-like element IS2 family transposase"
     gene            complement(1009..1158)
                     /locus_tag="E3B71_RS27695"
     CDS             complement(1009..1158)
                     /locus_tag="E3B71_RS27695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_167776965.1"
                     /translation="MPSEHDEQLDSTDTQLPGEYDLFDLMAQVTEENLHALVDFGPPVG
                     REAL"
                     /name="hypothetical protein"
     gene            2243..2560
                     /locus_tag="E3B71_RS27440"
                     /old_locus_tag="E3B71_25590"
     CDS             2243..2560
                     /locus_tag="E3B71_RS27440"
                     /old_locus_tag="E3B71_25590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="WP_032242326.1"
                     /translation="MKKIFILSAALIMAGCATKQYPQAVNVTGPESSAMDCKAVALEIA
                     KTRSVQQEIEETGEFDGRTVLGFLGDFGIGNGMAKGEARKKAANRLNQLEALKAAKCAD
                     Q"
                     /name="lipoprotein"
     gene            3744..3878
                     /locus_tag="E3B71_RS27450"
                     /old_locus_tag="E3B71_25600"
     CDS             3744..3878
                     /locus_tag="E3B71_RS27450"
                     /old_locus_tag="E3B71_25600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072003336.1"
                     /translation="MHEPPVQSDRCVLSGNYRLESNPERHAKTPLAAAIGKQDVREMQ"
                     /name="replication initiation protein"
     gene            complement(4308..4676)
                     /locus_tag="E3B71_RS27460"
                     /old_locus_tag="E3B71_25610"
     CDS             complement(4308..4676)
                     /locus_tag="E3B71_RS27460"
                     /old_locus_tag="E3B71_25610"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032242306.1"
                     /translation="MFSYLKAMYHQSKIQAELKAQIHEQTTVNAICHHPESIEIIAVCS
                     TDAYYRKRKDAAFLTTCSVLMRTLKDESVPMVLRKTAWRLLNERYQRIKLNQAYRIENF
                     LLVADFEYALEEHDELAE"
                     /name="hypothetical protein"
     gene            5021..5257
                     /locus_tag="E3B71_RS27470"
                     /old_locus_tag="E3B71_25620"
     CDS             5021..5257
                     /locus_tag="E3B71_RS27470"
                     /old_locus_tag="E3B71_25620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_032242304.1"
                     /translation="MTEMERQLLSALESLQTSYEQQKQAWQDSYSSLQRMFETTSQALE
                     RNDRVCLNLSNQVNALHEQVERLSSDLSRLTRS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            5402..5734
                     /locus_tag="E3B71_RS27475"
                     /old_locus_tag="E3B71_25625"
     CDS             5402..5734
                     /locus_tag="E3B71_RS27475"
                     /old_locus_tag="E3B71_25625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_032242302.1"
                     /translation="MSHAIEFIETTMFTRQIKQIATDDEIKELQKELIESPDKGDLIQK
                     TGGLRKIRMATGNQGKSGSARVIYFLATAEVIYLVMAYPKSTKDSITDAEKAELKKLTQ
                     RLKDEV"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            5736..6020
                     /gene="nadS"
                     /locus_tag="E3B71_RS27480"
                     /old_locus_tag="E3B71_25630"
     CDS             5736..6020
                     /gene="nadS"
                     /locus_tag="E3B71_RS27480"
                     /old_locus_tag="E3B71_25630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="WP_032242301.1"
                     /translation="MSFFDELKASLEEAVEIKNGTKAPARVTRYELADVRAIRENLNIT
                     QSEMAKVLGTSLDTIKSWETGRRNPTGLAAKVLATIQANPKFFDELAAH"
                     /name="nadS"
     gene            6219..7095
                     /locus_tag="E3B71_RS27485"
                     /old_locus_tag="E3B71_25635"
                     /pseudo=""
     CDS             6219..7095
                     /locus_tag="E3B71_RS27485"
                     /old_locus_tag="E3B71_25635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946055.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            7240..>7504
                     /locus_tag="E3B71_RS27490"
                     /old_locus_tag="E3B71_25640"
                     /pseudo=""
     CDS             7240..>7504
                     /locus_tag="E3B71_RS27490"
                     /old_locus_tag="E3B71_25640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558268.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element transposase"
                     /name="IS1-like element transposase"
     gene            complement(7597..9141)
                     /locus_tag="E3B71_RS27495"
                     /old_locus_tag="E3B71_25645"
     CDS             complement(7597..9141)
                     /locus_tag="E3B71_RS27495"
                     /old_locus_tag="E3B71_25645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="WP_201360865.1"
                     /translation="MKNYDVFYGGIFKTITDYKLLKIQKDIKNNMTALVSSFKWVGIGQ
                     LVKIATQLVSLFYLTHLIPPSSYGLLAMSSVVMNLASIFNDMGTGAAVIQRQEASIGFY
                     NYIYRINIFTGLFVMLLVIITSPLIVVYFNTPELYPVLFLLAFSFPISSLTITHKAKLE
                     KEMNFKVIIAIEIFSSLTGMVLAIVLANLGFGVYSLIFQTLTTISISTICFLKVSGLSL
                     SLRIKNISHSDKSGVIGFSGYLLSFNLINYFSRNLDSILIGRFFSSAILGVYSIAYRIM
                     LFPLQSLTLVFSRVFLPHLSKNINNTNNTNKQDYLNALKLILSLSAPLMLGLSATSFSF
                     TRIFFDPKWHLLGDLLHWLAPTAIIQSALSTTGSVFMAYGKTRWLFFLGCIGAFLMGLS
                     FIIGVHFDIKTLVYLYFFANLINSLPAFILLKNILKFSYIELVNVFFRTISPAFLMFLL
                     IKVWQHFSFFKNASIDFSFNVALGIFSYIVFYTLFNFNEVVRTGVIHKLRCMTKLK"
                     /name="lipopolysaccharide biosynthesis protein"
     gene            9300..9997
                     /locus_tag="E3B71_RS27500"
                     /old_locus_tag="E3B71_25650"
     CDS             join(9300..9549,9549..9997)
                     /locus_tag="E3B71_RS27500"
                     /old_locus_tag="E3B71_25650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_223368647.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRASARIMGVGLNTVLRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEHWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1 family transposase"
     gene            complement(10456..11439)
                     /locus_tag="E3B71_RS27505"
                     /old_locus_tag="E3B71_25655"
     CDS             complement(10456..11439)
                     /locus_tag="E3B71_RS27505"
                     /old_locus_tag="E3B71_25655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="WP_151166972.1"
                     /translation="MKKKFSIIVPVYNVEKYLRECIQSVLDQDFNDYEIICVNDGSTDG
                     SLDIILSFDDQRIKVINQENKGLSVARNVAIESSTGEYIWFLDSDDKISHGSLKLIDKI
                     TNEKNYGIIFFNTHVFFDTPELKKKGWHDYKRSTFGEMDALTFFEKEMTNKKLIVSACC
                     YVFRRELLNGNAFIPHIYHEDNHFIATLIFDNYSTICLSLDEALYLRRVRNDSIMTSKK
                     SMRHIIGYSTCIDELTKYKKTKGIQNKSFDRFVNGMVLSLFSIAETIKTKEAMNYVARY
                     KKHPDLSFKDRALINYPLIKKTYLKLKNGKVFNYLRNGIINKKSNP"
                     /name="glycosyltransferase family 2 protein"
     gene            complement(11524..11892)
                     /locus_tag="E3B71_RS27510"
                     /old_locus_tag="E3B71_25660"
     CDS             complement(11524..11892)
                     /locus_tag="E3B71_RS27510"
                     /old_locus_tag="E3B71_25660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_020936656.1"
                     /translation="MANFNPGPRTAIAAVRDSKNDIETWKKNGIRREEIVTVLNERYGL
                     NISLVAFDKALYRLRKSTDEPSLADVDSVSAAETNFSEASGPDNGFFLSGNDFMRAEEE
                     SQRVVESFRDSLSRKKGF"
                     /name="hypothetical protein"
     Typing          complement(1194..1367)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.143"
                     /coverage="100.0"
                     /evalue="5.58e-78"
     Typing          1334..1842
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.51"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          3487..3852
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.185"
                     /coverage="89.0"
                     /evalue="2.91e-169"
ORIGIN
        1 gaagcagcgc ccatacccga cgataaccat acgtgggcag ctctccgata acatggtgta
       61 tacggagaag cacatccgta tcatcagtgt gacgactgcg gcggccatcc atccagtcat
      121 cggttcgtct gagaatgacg tgcaactgcg cacgcgacac ccggagacaa cggctgacta
      181 agcttactcc ccatccccgg gcaataaggg cgcgtgcgct atccactttt ttgcccgtcc
      241 atattcaacg gcttctttga ggagttcatt ttccatcgtt ttcttgccga gcaggcgctg
      301 gagttcttta atctgcttca tggcggcagc aagttcagag gcaggaacaa cctgttctcc
      361 ggcggcgaca gcagtaagac ttccttcctg gtattgctta cgccagagaa ataactggct
      421 ggctgctaca ccatgttgcc gggcaacgag ggagaccgtc atccccggtt caaagctctg
      481 ctgaacaatt gcgatctttt cctgtgtggt acgccgtctg cgtttctccg gccctaagac
      541 atcaatcatc tgttctccaa tgactagtct aaaaactagt attaagacta tcacttattt
      601 aagtgatact ggttgtctgg agattcaggg ggccagtcta gtcttacatt tgtctcataa
      661 tagtccaaaa taagagtaaa acgcaaaaaa tgtcttacaa ttgtcttatt tttgtccttt
      721 ccggttcatg gatacatgaa ccgcctgatg tggaacgctt tttatcgatc tcatgcgctg
      781 ccaaagatag attcattctt taaaagtcag gttacattat cccttaaatg gtcatacacc
      841 atacgcaacc gcgattttca tcgcaattgc atgaattaaa agataaaaac tgaagtcccc
      901 gtttatttat gtaatacgca acgtattaca accacctgta attacatgaa aataaagaaa
      961 taagatgtaa taccggcgta ttaccacgta cgcgggtaag cgaaaaagct ataacgcttc
     1021 ccggccgaca ggcggcccaa aatccaccag ggcatgcagg ttctcttcag taacctgcgc
     1081 cattaaatca aaaaggtcat attcgccggg aagctgcgta tcagtcgaat ccagttgttc
     1141 atcatgttca gatggcatgg ggtgatatcc agtagctgag aggttgttag agggtttcgg
     1201 ggcgcagccc tgaaccagtc acgtagcgct agcggagtgt atactggctt actatgttgg
     1261 cactgatgag ggagtcagtg aagtgcttca tgtggcagga caaaaaaggc ggcaccggtg
     1321 cgtcagcaga atatgtgata caggatatat tccgcttcct cgctcactga ctcgctacgc
     1381 tcggtcgttc gactgcggcg agcggaaatg gcttacgaac ggggcggaga tgtcctggaa
     1441 gatgccaggg aaatacttaa cagggaagcg atagggccgc ggcaaagccg tttttccata
     1501 ggctccgccc ccctgacaag catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc
     1561 cgacaggact ataaagatac caggcgtttc cccctggcgg ctccctcgtg cgctctcctg
     1621 ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg tctcattcca
     1681 cgcctgacac tcagttccgg gaaggcagtt cgctccaagc tggactgtat gcacgaaccc
     1741 cccgttcagt ccgactgctg cgccttatcc ggtaactatc gtcttgagtc caacccggaa
     1801 agacacgaca aagcgccact ggcagcagcc actggtaaca ggattagcag agcgagttaa
     1861 tgtaggtggt gctatagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     1921 agtatttggt atctgcgctc tgctgaagcc agttaccagg ttaagcagtt ccccaactga
     1981 cttaaccttc gaaaaaccac ctccccaggt ggttttttcg ttttcagagc aagagattac
     2041 gcgtagaaaa aaaggaactc aagaagatcc tttctatgct caaccaccgc atggcccata
     2101 gccgcgctgg ccgttgccgg tcagctccag aatcctcgac caatatggaa ccttgcccct
     2161 cccaaaattc aactaaaaac ccctcatttc ctgccgataa aatgggtatc tcttaactat
     2221 gcctaaataa ataaggataa ttttgaaaaa aatattcatc ctatcggcag cgctgatcat
     2281 ggcaggatgc gcaacaaagc aatatccaca ggccgtgaac gtcaccggtc cagaatcttc
     2341 cgcaatggat tgcaaagcgg tagctctgga aatcgccaaa acgcgcagtg tacaacaaga
     2401 aattgaggaa accggcgaat ttgacgggcg caccgtatta ggtttcctgg gtgattttgg
     2461 gataggaaat ggcatggcaa aaggcgaagc ccgcaaaaaa gcagctaacc ggctaaatca
     2521 gctggaagcc ctgaaggctg caaagtgtgc agatcaataa ggtacaaaaa agccctcggg
     2581 gctttttttg tacctgccaa tcagctgaat ttaccataac atccgttgcg tgcaccatct
     2641 cagacgccct cagggcattt ctgcgccctc ctgcctattt ccggcatctt tccctgtcca
     2701 tatcgtgaaa aaacgcgcct gatgatttct tcgcgtctgc atggctatgc acacgaacgc
     2761 atggacgtca ttttagcggc taaaatccgg cgagcgccct gaaagccacc acgaacccca
     2821 ccgataacgg tgaaagggtg cgcagtttac tgtgcgaccg tgaggcgttg gccaggccgt
     2881 caggcatggc aggatttatt gctaaggggg tcggatttaa cctggattaa tcagcacaca
     2941 gtgatgaacg ggattcggaa ggggcaccct tgcggcgcgg cgatgtatac atcgctaacg
     3001 tgccttggtc atttattgca agaagggaga ggtagcattt tgatagcaaa aaagtagcaa
     3061 aaggatagca accgaaagac cagaagactc tggagatagc aaaaaagtag caaaatggta
     3121 gcaaaaagat agcaaacatg cgggagctaa ccaggagtct ttagcgggcc ggcggtactc
     3181 caaaaacaca ttttgtcccg ctgttttgtg aataacccag catagctgtg ggcacaaaac
     3241 atttcctgtc cgctgcttta acctttgtaa tccaggaacg tcaggtgacg tcactttgcg
     3301 gcgttagccc tatcctgcat cctatgaaaa aatgcagtta cggagtgtaa ggtgccggac
     3361 gtgaggccga agatattccg cttcctcgct cactgactcg ctacgctcgg tcgttcggct
     3421 gcggcgagcg gacggagaat attcacaaag gcatatggcg atgaagacga gataaagcga
     3481 gggaggaaag gccgcggcgc tgccggtttt ccataggctc cgcccccctg acaagcatca
     3541 cagaatctga cgctcaaatc agtggtggcg aaacccgaca ggactataaa gatcccaggc
     3601 gtttccccct ggtagctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt
     3661 cattccgctg ttatggccgc gtttgtctca ttccacgcct gacactcagt tccgggtagg
     3721 cagttcgctc caagctggac tgtatgcacg aaccccccgt tcagtccgac cgctgcgtct
     3781 tgtccggtaa ctatcgtctt gagtccaatc cggaaagaca cgcaaaaacg ccactggcag
     3841 cggccattgg taaacaggat gtgcgagaga tgcagtaaga gttcttgaag tgatggcctg
     3901 agtgcggcta cactgaaagg acagatttgg tgacatgtct cgcacaagac tgttaccgcg
     3961 gttagaaaat ctgccaggat tagctaacct tcgaaaaacc acctgccagg gtggtttttt
     4021 cgtttacaga gcaagagatt acgacgcaac agaaaggatc tcaagaagtt ccttgctgta
     4081 ttgcgggtcc gcttggaaaa aggaaattat cctacgttaa gcgttttaag ctaacgggtg
     4141 atttcgttcc attgagcgcg aacccctgac cgtaagggat gtacgaaatg atccggctgt
     4201 ccgttaatgg ttttgcttac tttcggacat gttgtctaca tgctgtacgt aatcggacgt
     4261 ttaagtcaca tgcacgttaa ctgtcggtat tcatcgcctc actagtacta ctctgctagt
     4321 tcatcatgtt cttcgagagc atattcaaaa tcggcaacaa gcaagaaatt ttctatccta
     4381 taagcctgat ttaacttaat tcgttggtat ctttcgttca ataatcgcca tgctgttttc
     4441 cgtagtacca tcggtacgga ttcatctttt aaagtacgca tgagcacaga acaggttgtc
     4501 agaaaagcag cgtccttacg ttttcggtaa tacgcatcag tcgaacaaac cgcaatgatc
     4561 tcgattgatt cagggtgatg acagatagca ttaaccgtag tttgttcatg tatctgtgcc
     4621 ttgagttcag cctgaatttt tgattgatga tacatagcct tgagatagct aaacatgatt
     4681 tccccatcaa acagtaagtc gtgtcaattc ctagagttaa cacatttctg atcatgactc
     4741 acacgttacg ttcagtcggt agccatctgt gcaaaaaatg cgcttagcgt aggataattt
     4801 cctttttcca agcggacccc agagcggcgc aggggtagcg atttacaagg acgacaagca
     4861 gaagaacacc tatctggtgg tactgccgaa gaaagcgcat ggcaccgaga catacacatc
     4921 gacagccgga aacaccgtga tgaagtacac cgcgaaataa ccccgtcaga caccacagaa
     4981 acgccacagc acgtttttat cactgaggac aggaaaaacc atgaccgaga tggaaaggca
     5041 gcttctgagc gccttagaaa gcttacaaac aagctacgaa cagcagaagc aggcgtggca
     5101 ggacagttac agcagcttgc aacgcatgtt cgagactacc tcacaggcac tggagcgcaa
     5161 cgacagggta tgtctgaact tgagcaatca ggtgaacgcc ttgcacgaac aggtcgaacg
     5221 cttgagcagc gatctgagcc gattaacacg ctcgtaaagc agcaggaacg gcagttacag
     5281 caggagaagc aagcaacccg ctatcgaggt cagtccatgt aacgtctgat ggcacagaac
     5341 gcggcttttc agaacacgcc tcttggtgta cattgtacat caaaatatac aggagacagc
     5401 cgtgagtcac gcgattgagt ttatcgagac aacgatgttt acccgtcaga taaagcaaat
     5461 cgccacggat gatgagatca aagagcttca gaaagagctt atcgagtcac cggacaaagg
     5521 tgacctcatc cagaaaacgg gcggtctgcg aaagatcagg atggcgacgg ggaatcaggg
     5581 aaagagcggc agtgcgcggg tgatttactt ccttgccacc gcagaggtga tttatctggt
     5641 gatggcctac cctaaaagca ccaaagacag catcacagac gcagagaaag cagaactgaa
     5701 gaagctgacc caacgactga aagacgaggt gtaagatgag tttttttgac gagctgaaag
     5761 cctctctgga agaggccgtt gaaatcaaaa acggaaccaa agcccccgcc cgtgtgaccc
     5821 gctacgagct ggcagacgtc agggctatca gagaaaacct gaatattact cagagcgaaa
     5881 tggcgaaagt gctgggaacc agcctcgata ccatcaaaag ctgggaaacc ggacgccgta
     5941 acccaaccgg actggcagca aaagtgctgg ccaccattca ggctaaccca aagttttttg
     6001 atgaactggc ggcgcattaa gctgatgtga cagaagaaat tcgacaggcg aggccgttcg
     6061 cgtgtaactt taacggcaac agaaaaaaca aagccccgat aatcatgatt tcaacttggc
     6121 ggaagagaca tgataaatca gggctaggta taaaacctaa gaaggatatt agcacatgaa
     6181 ccgtgccaga caacagcccg ccataagttc agggcacatt gcgtaacgca gcactcgaac
     6241 ttttcaacga ccgcttgccg cataaaccgt acttctcgga tgatttacac tttggtgtcc
     6301 gcatcgcagg gaaagagcgc gctattctgg ctaaatacat ccagtttaat cagcctcacg
     6361 ccatgttctg gctgggattt gacgttgacc gcatcggcgc agccattgac tggagcgacc
     6421 gcaacgcccc cgcgccgaca ctgaccatca ccaaccccga aaacggacac gcgcacctgc
     6481 tgtacgcact gaaaacctcg atacgcaccg cgccagacgg aaaaatgaaa ccgttacgtt
     6541 acgccgctgc tgtagagagc gcgctacgta aaaaattggg cgctgacacg gggtattcgg
     6601 ggctaatttg caagaatccg aaccacagtc actggaaaat tgcccgtatg gcagcctgaa
     6661 ctctacacgc tggactggtt agccgaatca ctcgacctga acgctgcgaa cgataaaaaa
     6721 atcgtcgccg attatggctt aggtcgtaac tgcatactgt ttgataaaac cagaaagtgg
     6781 gcatatcgcg ctatccgtca gggctggcct gagtacgaac agtggctaca agcctgccat
     6841 gaacgcgcct gcgcttacaa tctgcaattt gcagtgcctt tgaatgagaa tgaagtaacg
     6901 ggaatagcga aaagtatctg caagtggaca tttttgaaat ttaataagca aaatttttca
     6961 cttatacaag ccaaaagagg aaaagttggt ggtgaaaaat catcaggagg aggtaggctg
     7021 tctctagggg agccttgggt tgctttaggt attagtcgcg ctacattttt tagaaaaagg
     7081 aaaatttata attaagattc agtattttac atactgaatc ttaattattg aatcaccatt
     7141 acttaagtgt tgatagtgtt ttttttccag cagtaacaac tgtttgtgat gatgccaact
     7201 tgctggttta gtgtctaatg gtgtttttga ggtgctcccg tggcttccat ttccatcagt
     7261 tatccctcct gttcagctac tgaaggcgtg gtgcgtaacg gtaaaagcac taccggacat
     7321 caacgctatc tctgctctca ctgtcgtaaa acatagcaag caactacagt tcacttacac
     7381 cgcctctgag cccggtacac accagaaaat cattgatatg gccatgaatg gcgttggatg
     7441 ccgggccaca tcccgcatta tgggcactgt cctcaacacg attttacgac acttaaaaaa
     7501 attaggccga agtcggtatc gggcattatc tgaacataaa acacaagcag taagttggag
     7561 tcattaccaa ttcatttaat gaatttaata aatgaatcac tttaacttgg tcatgcatct
     7621 taacttatga attactcctg tcctaacaac ctcattgaaa ttaaacaagg tataaaaaac
     7681 aatataacta aaaataccca atgcgacatt aaaagaaaaa tcaatgctgg catttttgaa
     7741 aaaggaaaaa tgctgccata ctttaataag taagaacatc aaaaaagctg gggagatagt
     7801 tctaaaaaac acgtttacca attcaatgta actaaacttc aagatattct taagtaaaat
     7861 aaatgccggg agtgaattaa ttaagtttgc aaaaaaataa aggtagacca atgtttttat
     7921 atcgaaatga acacctataa tgaaagacaa ccccatcaaa aaagcgccaa tgcaacctaa
     7981 gaaaaacaac caccttgttt ttccgtaggc cataaaaaca gaacctgttg tactcaaggc
     8041 tgattggata atcgctgtag gtgccaacca atgcaataag tctccaagta ggtgccactt
     8101 aggatcaaag aaaatcctcg taaaagaaaa agatgtagca gataaaccta gcatcagtgg
     8161 cgcgcttaat gatagtataa gttttaatgc atttaagtaa tcttgtttat tagtgttatt
     8221 agtgttattt atgtttttgc taaggtgtgg caagaaaact ctagaaaaaa caagcgtcag
     8281 gctctgcaac ggaaataaca taatgcggta ggcaatcgaa tagaccccaa gtattgcgga
     8341 tgagaaaaaa cggcctatta aaatagaatc aagattcctg gaaaaataat ttattaaatt
     8401 aaaagacaat aaataaccac taaaccctat aactcctgac ttgtcactat ggctgatatt
     8461 tttaattctc aatgataaac tcaacccgga aaccttcaaa aaacatattg tggatattga
     8521 tattgttgtc agtgtttgaa aaatcaaact ataaacacca aagcctaaat tagctaaaac
     8581 tattgccagc accattcctg taaggctaga gaaaatttca attgcaatga tgactttaaa
     8641 gttcatttct ttttctaatt ttgctttgtg agttattgtt aagctggaaa ttggaaagct
     8701 aaaagccaat agaaatagaa ccgggtatag ctcgggtgtg ttaaagtaca cgacaattaa
     8761 tggggatgtt attattacaa gcagcataac aaacaaccca gtaaaaatgt ttatcctgta
     8821 tatgtaatta taaaaaccga tcgacgcctc ttgtcgctga attactgccg ccccagttcc
     8881 catgtcattg aatatgcttg ctaaattcat aaccacagat gacattgcta atagtccata
     8941 gctcgatggg gggattaagt gcgtcaagta gaataacgaa accaattgtg ttgctatttt
     9001 tacaagttgt ccaatgccaa cccatttaaa actactgact agtgcagtca tattgttttt
     9061 aatatccttt tgaattttca ataatttata atccgtgatg gttttaaaga taccaccata
     9121 aaagacatca taattcttca agctatcatc tacgttgtag attccgattt agctaaactt
     9181 taggacttta taagttcgta tcccgtatga ttataaggca ttatgatcat ctccaccaat
     9241 agtgggtaat gctgccaact tactgattta gtgtatgatg gtgattttaa ggtgcttgcg
     9301 tggcttccat ttccatcaga tgtccttcct gctccgctac tgaaggcgtg gtgcgtaacg
     9361 gcaaaagcac tgccggacat cagcgctatc tctgctctca ttgccgtaaa acatggcaac
     9421 tacagttcac ttacaccgcc tctcagcccg gtacgcacca gaaaatcatt gatatggcca
     9481 tgaatggcgt cggatgtcgc gccagtgcac gcattatggg cgttggcctc aacacggttt
     9541 tacgtcactt aaaaaactca ggccgcagtc ggtaacctcg cgcatacaac cgggaagtga
     9601 tgtgattgtc tgcgctgaaa tggacgaaca ttggggctac gtcggtgcta aatcacgtca
     9661 gcgctggctg ttttacgcgt atgacaggat acggaggacg gttgtggcgc acgtcttcgg
     9721 tgaacgcact ctggccacac tggagcgtct tctgagcctg ctgtcggcct ttgaggtcgt
     9781 ggtatggatg acggatggct ggccgctgta tgaatcacgc ctgaagggaa agctgcacgt
     9841 tatcagcaag cgatatacgc agcgcattga gcgacataat ctgaatctga gacaacatct
     9901 ggcaaggctg ggacggaagt cactgtcgtt ctcaaaatcg gtggagctgc atgacaaggt
     9961 catcgggcat tatctgaaca taaaacacta tcagtaagtt ggagtcatta cccggtctgc
    10021 gttgtcggga agatgcgtga tctggggtta gtttggatat agagaattat tgtacggtaa
    10081 gcctgttttt tgaacagact tacctatagc aatagggtgt actcccgcgc tatacggcat
    10141 tttcagaggt ttgaccttaa tcggataacc taaatgcgat actgcaagag cgacaactta
    10201 aaagcgatag ccactgacgc aaaatcaacc ccttacgaac caagattggt taaataatgc
    10261 gcttaacgta caaaaatttc cgatctccaa actgaccccg atctgatcct tcaactcagc
    10321 aaaagttcga tttattcaac aaagccccgg aaagcgtgcg tatcgcctga aaacccaacc
    10381 cgtcacgggg atacctatcc gaaatcttat ttattcaaca aagaaaaacc tttattaatt
    10441 atcgctttgc ttttattaag ggttgctttt tttattaata atgccattcc ttaggtaatt
    10501 aaacaccttc ccgtttttta gttttaaata agtcttttta attaatgggt aattaattaa
    10561 tgctctatct ttaaatgata ggtcggggtg ttttttatat ctcgccacat agttcattgc
    10621 ttcctttgtt tttattgttt ccgctataga gaataacgac aaaaccattc cattcacaaa
    10681 tctatcaaag ctcttgtttt gtatgccttt tgttttttta tacttcgtaa gctcatcgat
    10741 gcatgttgaa tatccaatga tgtgcctcat tgatttttta gacgtcatta tggaatcatt
    10801 tcttaccctc ctaagatata acgcctcatc caacgagagg catatagttg aataattgtc
    10861 aaaaatcagg gttgctataa agtgattatc ctcatgatat atatgcggaa tgaatgcatt
    10921 gccatttaat aattcccttc taaaaacata gcagcatgct gacactatta gttttttgtt
    10981 cgtcatttct ttttcaaaga aagttaatgc atccatttca ccaaatgttg aacgcttata
    11041 atcatgccag cctttttttt tcagttctgg agtgtcaaaa aaaacatgtg tattaaagaa
    11101 aataatccca taattttttt cattggttat cttgtctatt aactttagac ttccatgtga
    11161 tattttatca tcactatcta aaaaccatat atattcacca gttgatgatt caatggcgac
    11221 atttcttgcc accgacaggc ctttattttc ctgatttatt actttgattc tttggtcatc
    11281 aaatgacagt atgatatcta gactgccatc tgttgaacca tcatttacac agattatctc
    11341 atagtcgtta aaatcctgat ctaatactga ttgaatacat tcccttaagt atttttcaac
    11401 gttatataca ggtactatta tcgaaaattt ttttttcaca gtaatctcca aacaatattt
    11461 agttatacta acatcaagtt gttatttatg gaatgcgatt tatcaacggc tttgttgaat
    11521 aaatcagaat cctttcttcc tgctaaggct atctctgaat gactcaacca ctctttgaga
    11581 ttcttcttct gctcgcatga agtcattccc ggacagaaaa aaaccattgt caggcccact
    11641 ggcttcagag aaattcgttt ctgcagcaga cacagaatcc acatcagcaa gagacggttc
    11701 atctgtactt tttctcaatc tgtaaagagc cttatcaaac gcaaccagtg agatattcag
    11761 gccgtaacgt tcattcagca cagtcacaat ctcttcccta cgaataccat ttttcttcca
    11821 ggtttctata tcgttcttgg aatccctgac agctgcaatg gctgttcttg gaccagggtt
    11881 aaaattcgcc atataccgcc cataatgtct ttggatttgc ccctatattt ccagacatct
    11941 gttatcactt aacccattac aagcccgctg ccgcagatat tcccgtggcg agcgataacc
    12001 cagcgcacta tgcggatgcc attcgttata atgctcgaac gcctctgcaa ggttctttgc
    12061 tgccgttaac ccgtctggtt tgggcatgat actgatgtag tcacgcttta tcgttttcac
    12121 gaagctctct gctattccgt tactctccgg actccgcacc gccgtgttct tcggttcaag
    12181 tcccaacatc cgggcgaact ggcgtgtttc attagcccgg tagcatgaac cattatccgt
    12241 cagccactcc actggagacg acggaagatc gttgccgaag cggcgttcca ccgctcccag
    12301 catgacgtcc tgtactgttt cactgttgaa gccgccggta gtgaccgccc agtgcagtgc
    12361 ctcacgatca cagcagtcca gcgcgaacgt gacacgcagt ctctctccgt tatcacagca
    12421 gaactcgaac ccgtcagagc accatcgctg attgctttct ttcacggcca ctctgcctgt
    12481 atgtgcccgt ttcgatggcg gtacagcagg ttttcgctca agcaacagcg cattctggcg
    12541 catgatccgg taaacacgtt tggcattgat cgcaggcata ccatcaagtt ctgcctgtct
    12601 gc
//
