LOCUS       NZ_CP042154            14156 bp    DNA     circular CON 08-OCT-2023
DEFINITION  Staphylococcus aureus strain B4-59C plasmid pSALNC14, complete
            sequence.
ACCESSION   NZ_CP042154
VERSION     NZ_CP042154.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12359201
            Assembly: GCF_007726525.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14156)
  AUTHORS   Neyaz,L., Karki,A.B. and Fakhr,M.K.
  TITLE     Comparative genomics of plasmid bearing Staphylococcus aureus
            strains isolated from various retail meats
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14156)
  AUTHORS   Neyaz,L., Karki,A.B. and Fakhr,M.K.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JUL-2019) Biological Science, The University of Tulsa,
            800 S Tucker Dr, Tulsa, OK 74135, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC Genomic workbench v. 7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 204.33x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/08/2023 02:16:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,799
            CDSs (total)                      :: 2,718
            Genes (coding)                    :: 2,627
            CDSs (with protein)               :: 2,627
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 53 of 91
            Pseudo Genes (incomplete)         :: 36 of 91
            Pseudo Genes (internal stop)      :: 35 of 91
            Pseudo Genes (multiple problems)  :: 30 of 91
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP042154.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..14156
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="B4-59C"
                     /isolation_source="retail chicken"
                     /db_xref="taxon:1280"
                     /plasmid="pSALNC14"
                     /geo_loc_name="USA: Oklahoma"
                     /lat_lon="36.1540 N 95.9928 W"
                     /collection_date="2010-06"
                     /collected_by="Lubna S. Abdalrahman"
     gene            complement(join(14147..14156,1..>488))
                     /locus_tag="FP482_RS14075"
                     /old_locus_tag="FP482_14070"
                     /pseudo=""
     CDS             complement(join(14147..14156,1..>488))
                     /locus_tag="FP482_RS14075"
                     /old_locus_tag="FP482_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /name="IS30 family transposase"
     gene            complement(590..721)
                     /locus_tag="FP482_RS14410"
     CDS             complement(590..721)
                     /locus_tag="FP482_RS14410"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012840548.1"
                     /translation="MYIIYLKKGFIDISKEGKMAKIGYARVSTKDQNLGSVVKLEKV"
                     /name="hypothetical protein"
     gene            1228..1782
                     /locus_tag="FP482_RS14080"
                     /old_locus_tag="FP482_14075"
     CDS             1228..1782
                     /locus_tag="FP482_RS14080"
                     /old_locus_tag="FP482_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001062591.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001062591.1"
                     /translation="MAKIGYARVSTISQSLETQINLLKAEGCIKIFSEKVSGKKTNRSK
                     LDECLKYLREDDVLVVTKIDRLGRTTKQLINLASWLQEHNIELKILDGNFNMNSPLGKM
                     IFTVMSGFAELETDLNRERTLEGLNEAKARGRKGGRPGVTKDVKNYVMYLYNNTELSGN
                     EIARKTGVSRSTVYRIKRDFN"
                     /name="recombinase family protein"
     gene            1795..2043
                     /locus_tag="FP482_RS14085"
                     /old_locus_tag="FP482_14080"
     CDS             1795..2043
                     /locus_tag="FP482_RS14085"
                     /old_locus_tag="FP482_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001632623.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_001004832.1"
                     /translation="MNENILLELCSKLKGIRKGKKYTQQEVADIIGINIWTVNRIENKK
                     LEEVKLKTILRILDLYEITLYEFIEDNKDIVNRAYNK"
                     /name="helix-turn-helix transcriptional regulator"
     gene            2435..4009
                     /locus_tag="FP482_RS14090"
                     /old_locus_tag="FP482_14085"
     CDS             2435..4009
                     /locus_tag="FP482_RS14090"
                     /old_locus_tag="FP482_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="WP_000932174.1"
                     /translation="MLNINKIYDFILHGSIEKYKFKNSKATDLEKANFKATQPRNWRSA
                     RIAVTPNKATLIDGLGLIIASEEALVQYGHKFSHWTPNPYNYLSKEKANIADVNTYKVL
                     NRTKDNVKQINTFVLDVDEDIEYNELVQSLASAHINEGAELPNLYVKTPRGWHLYFVLD
                     TPFYAKGIEKKAIKVAEKVHSSLAHAISKYLPVDNQCVSTGYFRLPTKDNVQLFTNQYC
                     SKSYMVRWSKEYSEKHNLNQNVLYKSIANGQELTPEWVDYLLKEADVTSFGYGVGRNNA
                     MFSVALYYYSKNIDMKVAESNIYAFNQRLNQPLSHKEVERVIKSAYSGRYGGAMREHVE
                     NLLEAFTDGQIAYSNSGVSINGFWKHKKERKDRVRSHFDERKADLERYLNEHTNKQKVF
                     VKGSMSAIAKKLGMARSSLYAVFEKHINKGTIIKYTIKNGRYSETFIALKSVYIESLFE
                     MVKSIKNKKESYITAIRHLVSSERNTHSLMVQNNGLLNEIENTLTYIDRHTQYIEGNEI
                     LYERFIS"
                     /name="primase C-terminal domain-containing protein"
     gene            4871..5239
                     /locus_tag="FP482_RS14095"
                     /old_locus_tag="FP482_14090"
     CDS             4871..5239
                     /locus_tag="FP482_RS14095"
                     /old_locus_tag="FP482_14090"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_041203784.1"
                     /translation="MKDFFYGDPWFTFLIYRENRRGKSKDSKLGFYLAGDKELMVSVRK
                     HFSNVYPNIEFHEADESHLDFPTKRSYFGYAKLKHNSLKQGYPIEIFQHDNLTKIVSIM
                     EPDTWLQFRFRRNYGFKL"
                     /name="hypothetical protein"
     gene            complement(5543..6274)
                     /locus_tag="FP482_RS14100"
                     /old_locus_tag="FP482_14095"
     CDS             complement(5543..6274)
                     /locus_tag="FP482_RS14100"
                     /old_locus_tag="FP482_14095"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019168209.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic endopeptidase"
                     /protein_id="WP_000753929.1"
                     /translation="MKKTTIYFVLYFIISALGFGMLKKLGIEYNSLEIAVYALPMMIIL
                     SIFVYSIALNPYQKRKSSPIIHVKSLEYVVPLVILCDILIILELLYTGNFRPILVAQLF
                     LTFLIGFSEEFFFRKYIIEHILTKYKSKLLALITSSILFGLLHMANTFGGLTIENAASQ
                     SMSATGVGLLYGLIYIFTKKIWMLVLLHMNVDFALFSSVINQSYLQAVPAFIVDIILIF
                     TVLKIIMYILKSIKSKNNKFL"
                     /name="CPBP family intramembrane glutamic endopeptidase"
     gene            complement(6387..7196)
                     /locus_tag="FP482_RS14105"
                     /old_locus_tag="FP482_14100"
     CDS             complement(6387..7196)
                     /locus_tag="FP482_RS14105"
                     /old_locus_tag="FP482_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002491360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_001106667.1"
                     /translation="MNYIKYIHSVDGTTLYVKVNENVNSSANIIICHGLAEHLDRYDEI
                     SNYLQEKNFNIIRFDQRGHGRSGGKRTFYSNVNEIVEDLDAVINFTKEHYKGNIYLIGH
                     SMGGYGSVLYSTKNPGKINGLIISGAVTRYNQKTFGDIDDTIDRNKYINYEIGEGVCSD
                     TFELEKYRLDALTEKKVSYGLIYTVLEGVKGLVENAQYFDDNILILHGKDDGLVHYSDS
                     LDFYKNISSSKKELHIYDGLQHEIFNERKKNKEIFSEIANWINNDLT"
                     /name="alpha/beta hydrolase"
     gene            7767..8036
                     /locus_tag="FP482_RS14110"
                     /old_locus_tag="FP482_14105"
     CDS             7767..8036
                     /locus_tag="FP482_RS14110"
                     /old_locus_tag="FP482_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002477818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001129937.1"
                     /translation="MAKSLKDLNKTSERVFKNGNSKAISLSKKTIRLANFDIGDTVEVQ
                     KMNGGLFITKKKESIEDRIKNFFQSGGKYTELEVDWEERVGREI"
                     /name="hypothetical protein"
     gene            8033..8371
                     /locus_tag="FP482_RS14115"
                     /old_locus_tag="FP482_14110"
     CDS             8033..8371
                     /locus_tag="FP482_RS14115"
                     /old_locus_tag="FP482_14110"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436882.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003723 - RNA binding [Evidence IEA]; GO:0004521 - RNA
                     endonuclease activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="WP_001022435.1"
                     /translation="MNIKQFDILYIDLNPTRGREKHNVRPCLVINNQMSIDGTNFVWVL
                     PITTRGLRYPTDIQLKTKKGLVSGVIDTVQIRALDLKARQYNYKDELQDNLKNDILKAI
                     KTYLKPTL"
                     /name="type II toxin-antitoxin system PemK/MazF family
                     toxin"
     gene            8546..9229
                     /locus_tag="FP482_RS14120"
                     /old_locus_tag="FP482_14115"
     CDS             8546..9229
                     /locus_tag="FP482_RS14120"
                     /old_locus_tag="FP482_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019168208.1"
                     /GO_function="GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="WP_000277644.1"
                     /translation="MYKNLNNIHQSHYLALIYIILLIIIPRYAILPFSNKLNINYDIIN
                     EWYFFVITTILMIYAIKIFKVKLQKDWLNIKNKKIGVFLTGIVGFFLILLLNVILILII
                     NQGRPVKSVNQNAIENISFLSQFFAPLLIVIIGPILEELLFRVYILEVLKNKIKLLPAI
                     LISISSFTVIHMHSLTEDWKTLIPYFSIGIVTVFIYLKKHNFYYILSIHVLNNLVAQIA
                     IWFLQ"
                     /name="type II CAAX endopeptidase family protein"
     gene            9808..10482
                     /locus_tag="FP482_RS14125"
                     /old_locus_tag="FP482_14120"
     CDS             9808..10482
                     /locus_tag="FP482_RS14125"
                     /old_locus_tag="FP482_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="WP_001106066.1"
                     /translation="MNYFRYKQFNKGVITVAVGYYLRYALSYRDISEILRERGVNVHHS
                     TVYRWVQEYAPIVYQIWKKKHKKAYYKWRIDETYIKIKGKWNYLYRAIDAEGHTLDIWL
                     RKKRDHQSAYAFIKRLIKQFGKPQMIITDQAPSTKVAMTKIVKVFKLNPNCHCTSKYLN
                     NLIEQDHRHIKVRKTRYQSLNTAKNTLKGIECIYGLYKKNRRSLQIYGFSPCYEISHML
                     AS"
                     /name="IS6 family transposase"
     gene            complement(10634..11044)
                     /locus_tag="FP482_RS14130"
                     /old_locus_tag="FP482_14125"
     CDS             complement(10634..11044)
                     /locus_tag="FP482_RS14130"
                     /old_locus_tag="FP482_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_114306678.1"
                     /translation="MKWSLEQNFHGNAPNLKPQKIQGCLGFLKSLGWENWNQSYQTLDI
                     GELYFSLLLDDKRHVITYMHEGGNGGGRHRIFTGKVCGAEYVRAARLTQFRLNKQKLAY
                     YKNNWYLSNKLKILFKKVNCFKLFVSYILFMF"
                     /name="hypothetical protein"
     gene            complement(11054..11287)
                     /locus_tag="FP482_RS14135"
                     /old_locus_tag="FP482_14130"
     CDS             complement(11054..11287)
                     /locus_tag="FP482_RS14135"
                     /old_locus_tag="FP482_14130"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001002995.1"
                     /translation="MNEKEIGIAFLNHFTQVEAYLEALGVPENHIADIKPIVIQNYPYE
                     RFLFDDLGDVTNVIIPFSKVIGSNLFGNIGYK"
                     /name="hypothetical protein"
     gene            complement(11319..12602)
                     /locus_tag="FP482_RS14140"
                     /old_locus_tag="FP482_14135"
     CDS             complement(11319..12602)
                     /locus_tag="FP482_RS14140"
                     /old_locus_tag="FP482_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002440692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_000083517.1"
                     /translation="MSQSLRFTKLYKFLFEDPTFKTLPAKAKLLYALISERQSLSKANA
                     KQHGIQSQFIDNEGRLFSIYTNQELMTKLNMSKPTVIKLKKQLIEFGLLEDVRLGNNQP
                     NRLYPKKPYDNYFYAYDVDEFYRLPHALFENPKYQSLAAESIIAYAIYLSRYEYSIYKN
                     HFSDENGAVYCCFSNDNMANILDVSTRKVERIKKELIVSSLMINKRASFGKSNRLYISL
                     PKASDDKEVKKCRTSYTYFSYTYSSDIDSNDMNDTYDNNIDEVHEINDSPQTNHSSHPN
                     HQHNTEDTFNDQQVEQEMLLQHLPDYVQLNLKHFSNTEIRCIKSVLNKAKASYNHHAPV
                     DERVTYEACTYAIGEALRRIKLLSRQKNENICDLEAYMMQTFKRVFVEHVLNDQQKLDD
                     TKTKTHDISQFEPKNAFQSMAKRMFLDV"
                     /name="replication initiator protein A"
     gene            12732..13523
                     /locus_tag="FP482_RS14145"
                     /old_locus_tag="FP482_14140"
     CDS             12732..13523
                     /locus_tag="FP482_RS14145"
                     /old_locus_tag="FP482_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111526.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WP_000111526.1"
                     /translation="MSVITIGNFKGGVGKTTVSTLLSYIGSERYDKRILLVDFDPQGNA
                     TQIMKRTYPEAIDEKQTFIDALKTGELEDSIINLSTKLSLLPADSSLANLSDIIAKTDI
                     IKKRYILKTVIDQIKKNYNFDYIFIDVPPTINSDFTNNAVYASDYIAMVFQTQQSAYES
                     SLSFVNFLRDRKKESNLPFELIGAIPVLIKKNGNIDEYILEISKKTFSNALFKNQIFQR
                     ERIKKFGAEGIRDKDVHDKRVLHMLEAIYKELIERIELIEG"
                     /name="ParA family protein"
     gene            13533..13775
                     /locus_tag="FP482_RS14150"
                     /old_locus_tag="FP482_14145"
     CDS             13533..13775
                     /locus_tag="FP482_RS14150"
                     /old_locus_tag="FP482_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002437031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_044290595.1"
                     /translation="MAGFLDNIKVVGQQKRGFLDDIDTSAVKYTENYKPMAKSSTMRVD
                     TLIKRRLSQMALDKDTSIKAISDKVLQEFLEKNNY"
                     /name="hypothetical protein"
     Typing          2436..3934
                     /name="rep_cluster_155"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(11866..12602)
                     /name="rep_cluster_2439"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.696"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agtttatcat cttcgatgaa actcataaat acttccaatg gtgttctata acctaaagat
       61 tttctaggac gatgattgat tttttgagca acactgatga tatgattgtc tgttactaaa
      121 ttgaaatcta gttctttatc taatccactg cgtctaagta ttccgttaga atgttcattt
      181 aagcctctct gtgatggcgt gcctggatca gcaaaaaata tagatatatc atgacagttg
      241 catattgttt tccagtttga aaactcctta ccacaatcaa aagtaattga tttaaatata
      301 tgagaaggta aagctgaaaa cattgagtgc agagcatttt ctatatcggt tgccttacga
      361 cctaagggtc gcaaggtgat aatcatcttg gagactcttt ctaccaaagt aatgattgca
      421 ctaccatgac gacgaccaac aatagtatct ctttcaagat gcccaaactc cttattgaat
      481 aaaggaaatt ctggttctgt tgcaaagtta gaaaaacatc taatttcctt ttttatcatt
      541 tcagtgttag ctagttaaac gaacactgac atgataaaat gatgattagc tatacttttt
      601 ctaactttac aacagaacct aagttttgat ctttagttga aactctagca taaccgattt
      661 tagccatttt cccctcctta ctaatgtcta taaaaccttt ttttaagtat attatataca
      721 ttgatttata gataagtaaa tagacattgt attaaatata aaaaggataa taaacacctt
      781 aaatataagg gatttactat ccttcttcgt actttataat atattttagt tactgttgtc
      841 tataaacctc cgttttatag acagtcaaaa agtactgttt ctaacataac ctagtcaaaa
      901 ccgatcggtt ttgaccaggg tatgacatta gaaaataata tatttcaata agtaaaataa
      961 aataataaaa aatatgcttg aataagtgct atttgtaagg attaaaggct tgtagaactg
     1021 aataaattct ataagccttt aactatattc gtgcctggtc aaaatatgtg ttaaaatcaa
     1081 agtgtcaaaa ttaaaggtaa aaagggtttt tgaccaagct gagagaagcc ctcagacgcc
     1141 gcgaaaaaat attttgaagc ccattatacg taaacgcaca cacaatcgtt catattaaag
     1201 ctatttataa aggaagtagg cgatattttg gctaaaatag gctatgcaag agtttcaaca
     1261 atcagtcaaa gcttagaaac acaaattaac ttattaaagg ctgaaggttg tattaaaata
     1321 ttttctgaaa aagtaagtgg caagaaaaca aatagaagta aattagatga gtgcttaaaa
     1381 tatttaaggg aagatgatgt attagttgtg actaaaattg atagattagg acgcacaact
     1441 aaacaattaa taaatttagc aagttggcta caggaacaca atatagaatt aaaaatatta
     1501 gatggaaatt ttaatatgaa tagcccacta ggaaaaatga tatttactgt aatgagtggc
     1561 tttgcagaac tggaaacaga tttaaataga gaaagaactc ttgaaggttt aaacgaagcg
     1621 aaagctagag gaagaaaagg cgggcgacca ggtgttacga aagacgtaaa gaactatgtt
     1681 atgtatctat acaataatac agagttatca ggtaatgaaa ttgcgagaaa aacaggtgta
     1741 agtagatcaa cagtatatcg tatcaaacgt gattttaatt aggggggaaa aaagatgaat
     1801 gaaaatatat tattagaatt atgttctaaa cttaaaggga taaggaaggg taagaagtac
     1861 acacaacaag aagttgctga cataatagga attaatatat ggactgtgaa cagaattgaa
     1921 aataaaaaat tagaagaagt taaacttaaa acaattttac gtatacttga tttatatgaa
     1981 atcacacttt atgaatttat agaagataat aaagatatag taaatagagc atataacaag
     2041 taacatttaa tttatttgca aatgtaccaa aattattgca atcgtcaata tagaagttta
     2101 tactagaatt aatcaaaaaa agatatgaaa aaagaccacg tgcaaagtta aaattgctac
     2161 caacattttt aacttctagc gcttgaaacg ttacctcact aacgtataca aacggttttc
     2221 gggtcttctt ccataccttt atgatttagg tatttttaaa ttattttgta tacctaaatt
     2281 ataggggcat gagagaagaa agtaaagaaa aagacgctac ttttctactt ttctacttcg
     2341 ctactcgact actttaaatg tatatggacg cctaaacaag gactggtaac gcttacaagt
     2401 gttatcagtc ttttttgttt aggaggtgca gacaatgcta aatattaata aaatttatga
     2461 ttttatatta catggaagca tagaaaagta taagtttaaa aattctaaag ctacagattt
     2521 agaaaaagct aattttaagg ctacacaacc ccgaaattgg agatcagcac gtattgctgt
     2581 cacacctaat aaagctacat taatagacgg tttaggctta attattgctt cagaagaagc
     2641 tttagttcaa tatggtcata aatttagcca ttggacgcct aatccatata attatttaag
     2701 taaggaaaaa gctaatatag cagatgttaa tacgtataaa gtgttaaaca gaacaaaaga
     2761 taatgtaaaa caaatcaata catttgtttt agatgttgat gaagatattg aatataatga
     2821 attagttcaa agcttagctt cagcgcatat caatgaaggc gctgaattgc ctaatttgta
     2881 tgtaaagacg cctagaggtt ggcatttata ttttgtatta gatacaccgt tctatgctaa
     2941 aggtatagaa aagaaagcga taaaagtagc tgaaaaagta catagtagct tagcacatgc
     3001 aatttctaaa tatttaccag tcgataatca atgtgtatct actggctatt tcagattgcc
     3061 tacaaaagat aatgttcagt tatttacaaa tcaatactgt tcaaaatctt atatggtgcg
     3121 ctggagtaaa gagtattcag aaaagcacaa tttaaatcag aacgtacttt ataagagtat
     3181 agccaatggc caagaattaa cgcctgaatg ggttgattat ttgttaaaag aagcagatgt
     3241 tacttcattt ggatatggcg ttggtagaaa taacgcaatg ttttcagtag cgttatatta
     3301 ttattctaaa aatatagata tgaaagtagc agaaagtaat atctatgctt ttaatcagcg
     3361 cttaaatcag cctttatcac ataaagaagt tgaaagagta attaaaagtg cttatagcgg
     3421 tcgttatggt ggcgcaatgc gtgaacatgt agaaaacctt ttagaagctt ttactgatgg
     3481 ccaaattgca tatagcaata gtggtgtaag tatcaatggc ttttggaaac ataaaaaaga
     3541 gcgtaaagat cgtgttagaa gccattttga cgaacgtaaa gccgatttag aaagatattt
     3601 aaatgaacat acaaataagc agaaagtgtt tgttaaaggc tcaatgagtg ctattgctaa
     3661 aaagttagga atggctagat catctttata tgcagttttt gaaaagcata taaacaaagg
     3721 aactattatc aaatacacaa ttaaaaacgg tagatattca gaaactttca ttgctttaaa
     3781 aagcgtgtat attgaaagtt tatttgagat ggttaaaagt attaaaaaca aaaaagaaag
     3841 ctatataacg gctataagac accttgtaag cagtgaaaga aatacacata gtttaatggt
     3901 acaaaataac ggtttgttga atgaaataga aaatacactc acttatatag atcggcacac
     3961 tcaatatata gaaggtaatg aaattctata tgaacggttt attagttaaa ttttaattgt
     4021 ccgatgtgcc attacatata tcagggtgtt tgtgcgtttc tgtaaataat atatataagt
     4081 gagtagttaa gtagttatgt agaataatag aaaaacgcct actaattaaa attagcaggc
     4141 gccaaaaaat taaaaattac ctaaaatatt ttaacaaaat ggagatctct ccccattaac
     4201 aattatatat tatgggtgta tattgcgttt gattatggtg tgaatatatg acgtattata
     4261 ctatgtatta agattaatat aattagatta tgctttgatt aaattcatta cataatccat
     4321 accataatga actacataac acataacatg atatacaaat taaatattgt ttttatagtc
     4381 tttttaaggt gaaatgtaat taaggatata aaacccggta tttttcgtga ctatttcctg
     4441 ttagattaaa taagattaca ttattcatag aataatatca tcatcttaat taatcgtaac
     4501 tggagaagac tttgtttttt atttgttaaa tgcgggggcg acatatccac tgtaccttga
     4561 caaccaaata caaacgggag gtatccacat atgacgtcta aacttcttag acaactctcc
     4621 ttattcatat cctatatcct tatcctttca ctactcatat ttttcacagc gcgagcatca
     4681 ctgatcgctg gtgcaatttt tgcaattgta ttaataatat tttctatgtt gttgcgatat
     4741 attatgaaaa taattaaaat acagaaaaat atggaatatt atagaatatt aacttatgag
     4801 ggagattttc ctaaatctga taaaattttt aaattattga aaaatattca ttcgactaga
     4861 agaaatattt ttgaaagatt ttttttatgg agatccttgg tttacttttt taatctatag
     4921 agaaaataga cgaggaaaaa gtaaagatag caaattaggt ttttatttag caggggataa
     4981 agagttaatg gtttcagtta gaaaacattt tagcaatgtt tatccaaata tagaatttca
     5041 tgaagcagat gaaagtcatt tagattttcc tactaaaaga tcatattttg gttatgctaa
     5101 gttaaaacat aattctttaa aacagggtta tcctatagaa atatttcaac atgataattt
     5161 aactaagata gttagtatta tggaacctga tacatggtta caatttagat ttagaagaaa
     5221 ttacggtttt aagttatgaa aagctataaa aaagactgat gaagatatta gagagcaaaa
     5281 atttaaagat cgggataatt atgaaaagga gatttggaaa gaaaccaaca aacgttttac
     5341 gtatagtgat gttgcttttg atacaacaat aagggttctg ttgcaaagtt aaattagtat
     5401 acaaatctat acaaaatcaa taaacaaaat gctgcaataa caatagatta tttcgggaga
     5461 tttggttatt ttctcatttc tctgtattta tattatatga tttttaactt tgcaacagaa
     5521 ccgtaatatt tctatgtatc ctctataaaa acttattatt tttagattta attgacttga
     5581 gtatatacat aattatcttt aaaacagtaa aaattaaaat aatatccaca ataaaggctg
     5641 gtactgcttg taaatatgat tgatttatta cagacgaaaa taaagcgaaa tcaacgttca
     5701 tgtgcaataa taccaacatc catatttttt ttgtgaatat atatattaaa ccatataata
     5761 atcctactcc tgtagctgac atagattgag aagcagcatt ttcaatagtt aagccaccaa
     5821 aggtatttgc catatgtagt aaaccaaaaa gtatagaaga agtaataagg gctaacagtt
     5881 ttgatttgta tttagtcaat atatgttcaa taatatattt tctaaagaag aattcttctg
     5941 aaaaaccaat aagaaaggtt aaaaataact gtgccactaa tatgggtcta aaattaccag
     6001 tatataataa ttctaaaatt attaatatat cacataatat aaccaatgga accacatatt
     6061 ccaatgattt tacatgtatt ataggagaac tttttctttt ttgataggga ttcaaagcta
     6121 ttgagtaaac aaatattgac aaaattatca tcataggaag ggcatatact gcaatttcca
     6181 aactattata ttctatccct aattttttca acataccaaa accaagagcg cttataataa
     6241 aatatagaac aaaatatatt gttgtttttt tcatcaaaat aatcctttct aatttcttct
     6301 gaagtaaaac ttttaaagat aaagctggac acttattttt aaactttgca acagaaccga
     6361 cttattcaaa tttatcaatc ggattctcaa gttaaatcat tatttatcca gtttgcaatc
     6421 tccgaaaaaa tttctttatt cttttttctc tcgttaaaaa tttcgtgttg taaaccatca
     6481 taaatatgta attctttttt tgaagaagaa atgtttttat aaaaatctaa agagtcactg
     6541 taatgaacca acccatcatc tttgccatgt aaaattaaaa tattatcatc aaaatattga
     6601 gcattttcca ctaagccttt tacaccttcc aaaacagtgt atatcaagcc gtaagatacc
     6661 tttttttcag ttaatgcatc taatctatat ttttctaact caaaagtatc actacacact
     6721 ccctctccaa tttcataatt aatatactta tttctatcaa ttgtatcatc aatgtctcca
     6781 aaagttttct gattatacct tgttactgct ccagatataa ttaaaccatt aatctttcca
     6841 gggtttttag tagaataaag tacagaccca tatcctccca tactatgccc aatcagatag
     6901 atattacctt tataatgttc tttggtgaaa ttaataacag catctaagtc ctcaactatc
     6961 tcatttacat tgctataaaa agttcgtttt cctccagacc ttccatgtcc tcgttgatca
     7021 aatcttataa tattaaaatt tttctcctgc aaatagtttg aaatttcgtc atagcgatct
     7081 aaatgctctg ctaatccatg acatataatg atattagcgc tactatttac attttcattc
     7141 actttaacat ataaagttgt tccatctaca ctatgtatat attttatata gttcattctt
     7201 ttaacctctc tatatttata attattttac cattaattaa cataaatggg taaaataatt
     7261 ataaatatag attctattgc aaagttaatt ttatggtatg gtattaacac aaagaaatat
     7321 tctacatgaa ctactgcagc tataaacaat ttgataaagg tagcaacgat atagttgatg
     7381 attgttgatt aagatagacg ttgagttatt gtgatatatg gaaagagaac ttagggttaa
     7441 ctttaaactg tatgttgata agatatatat caaaattaaa taagaagggc aatgtccgaa
     7501 ttaattcaag atattaacct taatattaac tgtcattata aatataaata ccttaacgaa
     7561 ctcattgagc aaaattatcg tcatactaga aagttaagaa aacgattacc ttaaacgaaa
     7621 aattaaatat ttacttttca cctttgtaaa aaatgcagtc gaggatagta aaagcgatgt
     7681 ttttgatttt tataaattat tcaacttcat atgtatttac tttggtaact acatttgtta
     7741 tgatgaacaa aagggggctg ataataatgg ctaaatcatt aaaagactta aacaagactt
     7801 cggaacgagt ttttaaaaat ggaaacagta aagcaatcag tttaagtaaa aaaactatca
     7861 ggttagctaa ttttgatata ggagatacag ttgaagttca aaaaatgaat ggtggtttgt
     7921 tcattactaa aaaaaaggag tctattgaag atagaattaa aaatttcttt caaagtggtg
     7981 gaaagtatac agaattagaa gtagattggg aagaaagagt tggccgagaa atatgaacat
     8041 taaacaattt gacatactct acattgattt aaatccaact cgtggacgtg aaaagcataa
     8101 cgtaaggccc tgcttagtga taaataatca gatgagtatt gatggcacta attttgtttg
     8161 ggttttaccc ataacaactc ggggtttacg ttatccaaca gatatccaac ttaaaacaaa
     8221 aaaaggatta gtatctggtg tgatagacac tgttcaaata cgcgctttag acctaaaagc
     8281 acgtcaatac aactataaag acgaactaca ggacaattta aaaaatgata tattaaaagc
     8341 gataaaaact tatcttaagc caacgttata aaggattttg ttgcaaagct aaaagtatac
     8401 tatagattta atttcgtaac agggcaaaat atagtattta ttataccttt ataaacttct
     8461 ttgcttctta aaatgggaat acaaagcaaa tccaaataac ttttaaacaa atacttagta
     8521 attataaaaa attcaggaga tttccatgta caaaaattta aataacattc accaaagtca
     8581 ctatttagct ttaatttata taatactact catcattatc cccagatacg caatattacc
     8641 gttttcaaac aaactaaata taaattacga tataattaat gaatggtatt tttttgttat
     8701 tacaactatt ttgatgatat atgccattaa aatatttaaa gtaaaactac agaaagattg
     8761 gttaaatatt aaaaacaaaa aaattggtgt ctttcttaca ggaatagtag gcttttttct
     8821 aattcttctt ttaaatgtaa tacttatatt aattattaat caaggcagac cagtaaagag
     8881 tgttaaccaa aatgccattg aaaatatttc atttttatct caattttttg caccattatt
     8941 aattgttatc attggaccta tacttgaaga attacttttc agagtatata ttttagaagt
     9001 tcttaaaaac aaaattaaat tactccctgc aatattaatt tctatatcgt cttttacggt
     9061 aatacatatg catagtttaa cagaagattg gaagacatta atcccctatt tttcaatagg
     9121 catagtaaca gtttttattt atttaaaaaa acacaacttt tattatatac tctctatcca
     9181 tgtactaaat aatttagtag ctcaaattgc tatttggttt ttacaataaa aacagcagta
     9241 ttttagttta ttatcaacat caaaatacac aattcaaaaa agagaggtta ggatatcccc
     9301 cacgagtact tatgcctcac ctccaaatga atgaagtctt cagaacgacc acaatatttg
     9361 tagtataata gctatgaggt gttagtggtc gtttttttat tgatttcaaa tttcaaggta
     9421 caaactgatc tagatgtttt ttattatttt ctctataaaa atattttttg atagctaata
     9481 ttttaaaatc atacaaccat acctattaaa atcattatct tctccactac tattaccatt
     9541 ctcacttatc gctacaagta caaatggaac caaaataact acatttccta taaacgtcaa
     9601 tgtaattata cttatctcca atttatttat atacttattc ttcgcaaaaa agttttactc
     9661 ctacacatat atctaaaaca gttatgttgc aaagttaaaa aataaacctc tagactcatg
     9721 tttaaaataa tacttcgtat taacttgcta gggttctgtt gcaaagttga attgatagta
     9781 taattttaac aaaaaggagt cttctgtatg aactatttca gatataaaca atttaacaag
     9841 ggtgttatca ctgtagccgt tggctactat ctaagatatg cattgagtta tcgtgacata
     9901 tctgaaatat taagggaacg tggtgtcaat gttcatcatt caacagtcta tcgttgggtt
     9961 caagaatatg caccgattgt gtatcagatt tggaagaaaa agcataaaaa agcttattac
    10021 aaatggcgta ttgatgagac gtatatcaaa ataaaaggaa aatggaacta tttatatcgt
    10081 gccattgatg cagaaggaca tacattagac atttggttgc gtaaaaaacg agatcatcaa
    10141 tcagcatatg cgtttattaa gcgtcttatt aaacaattcg gtaaacctca aatgataatt
    10201 acagatcaag caccttcaac gaaggttgca atgactaaaa tagtgaaagt gtttaagctg
    10261 aaccctaact gtcattgcac atctaaatat ctcaataacc tcattgaaca agatcatcgt
    10321 catattaagg tacgaaagac aaggtatcaa agtctcaata cggcaaaaaa tacactcaaa
    10381 ggcattgaat gtatttacgg attatataaa aagaaccgta ggtctcttca gatctatgga
    10441 ttttcgccat gctacgaaat cagtcatatg ttagctagtt aaactaacac tgaaatgata
    10501 aaaaaggaaa ttagatgttt ttctaacttt gcaacagaac cgatgataaa ctgtccagct
    10561 taatttgaca atctaaatat aataaacatg tgctcactaa taataatatt tttttcatga
    10621 aaagatactc ctttcaaaac ataaataaaa tatacgaaac aaacaatttg aaacaattca
    10681 cctttttaaa taatatcttt aatttgttgc tcaagtacca attattttta taatatgcca
    10741 atttttgttt atttaatcta aattgtgtca atctcgcagc tctgacatat tctgcaccac
    10801 agactttacc agtaaatatt cgatgtctac cgccgccatt gccaccttca tgcatatagg
    10861 ttatcacatg ccttttgtca tcaagtagca aactaaaata gagttctcca atatccaatg
    10921 tttgataact ttgattccaa ttttcccaac ctaacgattt tagaaaacct aaacagcctt
    10981 gtattttttg gggtttaagg ttaggcgcgt taccatgaaa gttctgttct agcgaccatt
    11041 tcactaagtc aaactacttg tagccaatat tcccaaataa gttactccca atgactttag
    11101 aaaagggaat gatcacattc gttacatcgc ctaaatcatc aaacaaaaag cgttcatagg
    11161 gatagttctg aatgactatc ggcttaatat ctgcgatatg gttttcgggc acacctaaag
    11221 cctctaaata agcctccact tgcgtaaagt ggttcaaaaa cgcaatccct atctcttttt
    11281 cattcacaac tatctctcct tcctaaatat taaagttatc aaacatcaag aaacatacgt
    11341 tttgccatcg attggaacgc atttttcggt tcaaattgtg aaatatcatg cgtttttgtt
    11401 ttagtatcgt caagtttctg ttggtcgttc agcacatgtt caacaaagac acgcttaaat
    11461 gtttgcatca tgtaggcttc tagatcgcag atattttcat ttttttgtcg gcttaatagt
    11521 ttgatacgtc gtaaagcttc cccaatagca taggtacagg cttcatacgt aacacgttca
    11581 tctacaggtg cgtggtgatt ataactggct ttcgctttat tcagtacaga tttaatacaa
    11641 cgaatttcag tattactaaa atgttttaag ttcaattgta catagtctgg taagtgttga
    11701 agtaacatct cttgttcgac ttgttgatcg ttaaacgtat cctctgtgtt gtgttgatga
    11761 tttggatgac ttgaatgatt tgtttgtggt gaatcattta tttcatgtac ttcatcaata
    11821 ttattatcat acgtatcatt catatcatta gaatcaatat cactagaata agtataacta
    11881 aaataagtat aactagtgcg acatttttta acttccttgt catcactagc tttagggagt
    11941 gaaatatata atctattcga tttaccaaaa gatgcacgct tattaatcat tagactcgaa
    12001 acaatcaatt cttttttgat acgttctact tttcgtgtac tcacatctaa aatgtttgcc
    12061 atattgtcat tagagaaaca acaatagact gcaccatttt catccgagaa atgattttta
    12121 tagatactat actcataacg actcagataa atcgcataag caatgataga ttcagccgct
    12181 aaagattgat atttgggatt ttcaaataaa gcatgaggaa gacgataaaa ttcgtcaaca
    12241 tcataggcat agaaatagtt atcatagggc tttttgggat ataaacgatt cggttgattg
    12301 ttaccgagac gcacgtcttc taataaacca aattcaatca attgtttttt tagtttgatt
    12361 accgttggtt tcgacatatt aagtttcgtc atcagttctt gattcgtata aatggagaac
    12421 aaacgcccct cattatcgat aaattgggat tgaatgccat gttgcttagc attagctttc
    12481 gataagcttt gacgctcaga aattaatgca tagagcaatt tggctttcgc tggtaaagtt
    12541 ttaaatgtag gatcttcaaa taaaaatttg tatagttttg taaaacgtag tgattgagac
    12601 ataataaaaa aacctctttc cttatttgtt tttgttagag ttgatttttt ggaattgtat
    12661 aacagtaact tgtgacatgt cacaagttac tgttataata tattatatta taacagtaag
    12721 gagtgttgta tatgtcagtt ataactattg gtaattttaa aggaggggta ggaaaaacta
    12781 ctgtatcaac tttattatca tatattggct ctgaaaggta tgataaaaga attctattag
    12841 ttgattttga tcctcagggg aacgctaccc aaattatgaa aagaacatat ccagaagcaa
    12901 tagatgaaaa acaaacattt atagatgctc ttaaaacagg ggaattagaa gatagtatta
    12961 taaatttatc aacgaaatta tctttattac ctgcagattc ttcactagct aatttgtcag
    13021 atattattgc aaaaacagat attataaaaa agcgatatat acttaaaacg gttattgatc
    13081 aaattaaaaa aaattataat tttgactaca tttttataga tgtaccacca acgataaatt
    13141 cagattttac aaataatgct gtatatgcaa gcgactacat tgctatggta ttccagacac
    13201 aacaatcagc gtatgaaagc agtttatcat ttgttaattt tttaagagat agaaaaaaag
    13261 aatcaaattt accgtttgaa ttaataggtg cgatacctgt attaatcaag aaaaatggta
    13321 atattgatga atatatattg gaaatatcta aaaagacttt ttcaaatgct ctgtttaaaa
    13381 atcaaatatt tcaaagagaa agaattaaga aattcggtgc agaaggtatt agggataaag
    13441 atgttcatga taaaagagtt ttgcatatgt tagaagcgat atataaagaa ttgattgaaa
    13501 gaatagaatt aatagaagga taggtgattg caatggcagg atttttagat aatattaaag
    13561 tcgtaggaca acagaaaaga ggatttttag atgacataga cacttcagca gtgaagtata
    13621 cagaaaatta taagccaatg gctaaaagtt cgactatgag agttgatact ctgataaaaa
    13681 gaagacttag tcaaatggcg ttagacaaag acacgtccat aaaggcaata tcagataaag
    13741 ttttacaaga atttttagag aaaaataatt attaatagaa gtggtttagt tttttagatg
    13801 ttcagatttg attttacaaa agaaattatc tcattatcat agaccaatta gttttcaaat
    13861 cattattttt aagttcttta attctaatat agagaagttt aaagagtgca ttctttaata
    13921 agaacgcact cttttttgtt attttgtagc agctagaata tatatttttt gcaaatatta
    13981 ttttttgccc tggtaattca tgatttttat agcgttcata aacatcttca tcttgatatt
    14041 tacaccattc aattagtaaa tccggttctg ttgcaaagtt aaaattatac tataaattta
    14101 actttgcaac agaaccgaaa atgctctgca ctcaatgttt tcagctttac cttctc
//
