LOCUS       NZ_CP041737             2317 bp    DNA     circular CON 03-APR-2024
DEFINITION  Enterobacter hormaechei subsp. steigerwaltii strain ME-1 plasmid
            pME-1d, complete sequence.
ACCESSION   NZ_CP041737
VERSION     NZ_CP041737.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12306076
            Assembly: GCF_007556795.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei subsp. steigerwaltii
  ORGANISM  Enterobacter hormaechei subsp. steigerwaltii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Chavda,K., Kreiswirth,B. and Chen,L.
  TITLE     Genomic sequencing of E. hormaechei subspecies steigerwaltii strain
            ME-1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Chavda,K., Kreiswirth,B. and Chen,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2019) HMH-CDI, Hackensack-Meridian Health Center
            for Discovery and Innovation, 340 Kingsland Street, Building 102,
            Nutley, NJ 07110, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-MAY-2019
            Assembly Method                   :: Unicycler v. v0.4.8-beta
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_007556795.1-RS_2024_04_03
            Annotation Date                   :: 04/03/2024 04:07:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,949
            CDSs (total)                      :: 4,833
            Genes (coding)                    :: 4,770
            CDSs (with protein)               :: 4,770
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 46 of 63
            Pseudo Genes (internal stop)      :: 9 of 63
            Pseudo Genes (multiple problems)  :: 17 of 63
            Pseudo Genes (short protein)      :: 1 of 63
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP041737.1.
            Bacteria and source DNA available from HMH-CDI.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Enterobacter hormaechei subsp. steigerwaltii"
                     /mol_type="genomic DNA"
                     /strain="ME-1"
                     /sub_species="steigerwaltii"
                     /db_xref="taxon:299766"
                     /plasmid="pME-1d"
                     /collection_date="2018"
                     /collected_by="CDI"
     gene            705..818
                     /locus_tag="FO617_RS25440"
                     /old_locus_tag="FO617_24980"
     CDS             705..818
                     /locus_tag="FO617_RS25440"
                     /old_locus_tag="FO617_24980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071789001.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPVRHAKAPLAAATGN"
                     /name="replication initiation protein"
     gene            complement(1199..1735)
                     /locus_tag="FO617_RS24985"
                     /old_locus_tag="FO617_24985"
     CDS             complement(1199..1735)
                     /locus_tag="FO617_RS24985"
                     /old_locus_tag="FO617_24985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002069891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023150440.1"
                     /translation="MKKVYTAIILIVLLCGGVLSANYIFLQRHMNEVLKEDPRNDGISV
                     WVYYKWFVNSSEINYDLRSVSAENSSLDVSRVMLQFAEKVKDYDFSKVYLSYRGKDKFY
                     LKGEYFKTLGQEYGIQNPVYTLRTIPENVYMLNGERAYSVWEGGLLGVMGKQMEDLSDF
                     SKAWYLDDFIKSMSD"
                     /name="hypothetical protein"
     gene            complement(2024..2260)
                     /locus_tag="FO617_RS24990"
                     /old_locus_tag="FO617_24990"
     CDS             complement(2024..2260)
                     /locus_tag="FO617_RS24990"
                     /old_locus_tag="FO617_24990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016947370.1"
                     /translation="MKRLINLTPAEKRFLDDAVAAAERASGKKLNQPNRHIVLNRARAQ
                     IESQRQAERQRSAREEERQQAEFTWSRPRAPRR"
                     /name="hypothetical protein"
     Typing          complement(174..352)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="84.066"
                     /coverage="83.0"
                     /evalue="3.53e-41"
     Typing          329..818
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.105"
                     /coverage="94.0"
                     /evalue="0.0"
ORIGIN
        1 agaacctgca gccgggaacg gccctgagaa acggtgaacg ggaacggatt ttaatccggt
       61 gccgtgaagc gttggcgggg tatcggggcg cagccctgag atatcgtttt ggtgccggca
      121 tcatgatggc ggtactccaa aaacacatct tgtcccgctt tcagtttctg aatttgcggg
      181 ttccgggcgc agcgccggac cagtcacgta gcgctagcgg agtgtatact ggcttaatat
      241 gttagttggg cgtggtggct cagggaagtg cttcatgtgg caggggaaaa atgcggctgc
      301 ggtgcgtaag tgcaaaatgc tgatgatggt gaaatgacgc ctcctcgctc actcggtcgc
      361 tacgctcctg ccgtgagact gcggcgggcg ttacctgctc acgaatgacg aggtaacgaa
      421 atgaagctga ggtcaagaag ggcccgaaaa aggatgcggc gctgccgttt ttccataggc
      481 tccgcccccc tgacaagcat cacaaaatct gacgctcaaa tcagtggcgg cgaaacccga
      541 caggacttaa agatcccagg cgtttccccc tggtggctcc ctcgtgcgct ctcctgttcc
      601 tgcctttcgg tttaccggtg tcattccgct gttatggccg cgtttatctc attccacgcc
      661 tgacactcag ttccgggtag gcagttcgct ccaagctgga ctgtatgcac gaaccccccg
      721 ttcagtccga ctgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
      781 atgcaaaagc gccactggca gcagccactg gtaactgatt tagaggagat agtcttgaag
      841 tcatgcgcca gttaaggcta aactaaacga acagttttgg tgactgcgct cctctaacta
      901 gccagttacc tcggttcaaa gagttgctgc tctgagaacc ttcgagaaaa ccgccttgct
      961 gggcggtttt ttcgtgtaca gagcaagaga ttacgacgca acagaaagga tctcaagaag
     1021 ttccttgctg tattgctact atcctagaga tctgacccat taggggcatt atcagatgac
     1081 ggccttgtaa tccacgggga gcaggtcaca catctaaagc atctacgagt aaaaacaccc
     1141 cagtgaccaa tgagcgattt gcgtgagcta atcgtcaatg tatactgatc gcttcaaatt
     1201 aatcgctcat gcttttaatg aaatcatcta agtaccaagc cttagagaaa tcactaagat
     1261 cttccatctg ttttcccatt acacccagaa gcccaccctc ccaaacacta taagctctct
     1321 ctccatttaa catatacacg ttttccggaa ttgttcttaa tgtatatacg gggttttgaa
     1381 taccatattc ctgcccaaga gttttaaaat actctccttt taagtaaaac ttatctttcc
     1441 ctctgtaact aagataaacc ttactaaaat cataatcttt cactttttca gcaaattgca
     1501 gcatcaccct actaacatcc agtgaactat tttctgcaga aacagaacga aggtcataat
     1561 taatctcgga agagtttaca aaccacttat aataaaccca aacgctaata ccatcattcc
     1621 ttggatcttc tttcagaacc tcattcatgt gcctttgaag aaaaatgtag ttggcagata
     1681 aaacaccacc acataagagt actattaata tgattgctgt atatactttt ttcatttcta
     1741 taatccatct caactgctct aaagcttacc ctgagatttt taagctaagg tgccataagt
     1801 tagcaaggaa ttaagcatcg atttaccata acgtcttctt acgcgcaccg cgaaaacggc
     1861 ctcagggcgt ttctgcgcct tcagccacaa aagagcacgt cttaccccca gaaaaccgcc
     1921 cggaatcgct ctggtgagtt ttacggtggc gaatgactat gcaccggagt gaataccacg
     1981 ctgtttcgcc ctgaactccc cacaaaaccg acgaaagtgg caatcagcga cgcggcgccc
     2041 gcggacgcga ccaggtaaat tccgcctgct gccgctcctc ctcacgcgca gatcgctgcc
     2101 gttctgcctg gcgctgcgat tcgatctgtg cacgagccct gttaaggaca atgtgcctgt
     2161 tgggctgatt cagcttcttg cctgacgctc tctcagcggc ggcaacagca tcgtcgagaa
     2221 atcttttctc ggcaggcgtc aggtttatca gtcgtttcat ggcgttctcc ggtcagaata
     2281 actctgcaca gtctgccaga cacggcgaac ctcatga
//
