LOCUS       NZ_CP041736             2496 bp    DNA     circular CON 03-APR-2024
DEFINITION  Enterobacter hormaechei subsp. steigerwaltii strain ME-1 plasmid
            pME-1c, complete sequence.
ACCESSION   NZ_CP041736
VERSION     NZ_CP041736.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12306076
            Assembly: GCF_007556795.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei subsp. steigerwaltii
  ORGANISM  Enterobacter hormaechei subsp. steigerwaltii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Chavda,K., Kreiswirth,B. and Chen,L.
  TITLE     Genomic sequencing of E. hormaechei subspecies steigerwaltii strain
            ME-1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Chavda,K., Kreiswirth,B. and Chen,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2019) HMH-CDI, Hackensack-Meridian Health Center
            for Discovery and Innovation, 340 Kingsland Street, Building 102,
            Nutley, NJ 07110, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-MAY-2019
            Assembly Method                   :: Unicycler v. v0.4.8-beta
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_007556795.1-RS_2024_04_03
            Annotation Date                   :: 04/03/2024 04:07:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,949
            CDSs (total)                      :: 4,833
            Genes (coding)                    :: 4,770
            CDSs (with protein)               :: 4,770
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 46 of 63
            Pseudo Genes (internal stop)      :: 9 of 63
            Pseudo Genes (multiple problems)  :: 17 of 63
            Pseudo Genes (short protein)      :: 1 of 63
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP041736.1.
            Bacteria and source DNA available from HMH-CDI.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Enterobacter hormaechei subsp. steigerwaltii"
                     /mol_type="genomic DNA"
                     /strain="ME-1"
                     /sub_species="steigerwaltii"
                     /db_xref="taxon:299766"
                     /plasmid="pME-1c"
                     /collection_date="2018"
                     /collected_by="CDI"
     gene            325..543
                     /locus_tag="FO617_RS24960"
                     /old_locus_tag="FO617_24960"
     CDS             325..543
                     /locus_tag="FO617_RS24960"
                     /old_locus_tag="FO617_24960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_182407767.1"
                     /translation="MFGFSSGALMTFNDDERHLLVSVVSGWLRRAEGDAGAMMLDAYRQ
                     ILSETEPAARAVMLEFLESVRIHYVSS"
                     /name="hypothetical protein"
     gene            complement(841..1026)
                     /locus_tag="FO617_RS24965"
                     /old_locus_tag="FO617_24965"
     CDS             complement(841..1026)
                     /locus_tag="FO617_RS24965"
                     /old_locus_tag="FO617_24965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032673404.1"
                     /translation="MTRQESAALNMAKFIRSQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEKDA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1392..1619)
                     /locus_tag="FO617_RS24970"
                     /old_locus_tag="FO617_24970"
     CDS             complement(1392..1619)
                     /locus_tag="FO617_RS24970"
                     /old_locus_tag="FO617_24970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     Typing          46..215
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.698"
                     /coverage="98.0"
                     /evalue="2.0600000000000002e-57"
     Typing          707..794
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="94.382"
                     /coverage="100.0"
                     /evalue="1.5000000000000001e-30"
     Typing          complement(2035..2481)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.767"
                     /coverage="94.0"
                     /evalue="5.5e-153"
ORIGIN
        1 cccgtaccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
       61 aatatctcct gtatcacata ttctgctgac gcaccgatgc cgcctttttc tcctgccaca
      121 tgaagcactt aactgacatc cgcatccgtg ccaacatatt aagccagtat acactccgct
      181 agcgctgcgt gactggtccg gcgctgcgcc cggaacccgc ctgtaccggt taagcagccg
      241 ttctggtctg actgcgattt ttttcattcc tgcccgctac cctgtaaacc tttcttctgc
      301 gttgccgtta acaggaggta agccatgttt ggtttttcct ccggagccct gatgactttt
      361 aatgatgatg agcgacacct gcttgtgtcc gttgtttcgg gctggctccg gcgtgccgaa
      421 ggggatgccg gagccatgat gctggatgca taccggcaga ttttgtctga aaccgaaccg
      481 gccgcaaggg cggtcatgct cgaatttctt gaatcagttc gcattcatta cgtttcgtcc
      541 tgagcatttc ctgaagcatt tcctgaattt ttgtccatac tcaatccagg cggccgtcac
      601 ggacggtggt cgtcgttcca tgatgctgtt atggaagatt gagagtaatc taacttgatt
      661 gagttccggg cgtgtcgccc ggtttttttt gctgtttccc ggtgtccgct cccggacagg
      721 atgtgcattt gtaataccgc gcacgcggcg gcatcacaaa taccccgttc agggctgcgc
      781 cccgacaccc cgccatcgcc tcacggccgc gcactgaatt gcgcacccgt tcaccgattt
      841 tcacgcatct ttctcgccta tctgtgcgtt cagcatggcg taaagcgctt cagcctgatc
      901 gtgcagatgc tcacagcagc cggctgcctc atccagatcc atctgttcga gccgttcgag
      961 gaggaggagt gtctgtgagc ggataaattt ggccatgttg agggcggcgg attcctgtct
     1021 ggtcactggt gattttcccc gtctggtgat gatttttgcg tgaaaagagc atgccgtcat
     1081 tctggtgcat agtcatgcat gccgttaaaa tttaacaggc gcgtttctga cgggttccgg
     1141 ggtggtttgt tgtggttttt gtcatggttc cgtcagaaac gcgctgagcg cgtctgaggc
     1201 ggtgcgggca acggatgtta tggtaaattc ttgggggctt gattataaaa taccgcactt
     1261 cctcttatgt cttgtgcttt ttagacaacc ctgaaagctt ttagctttgg tgatgcaatt
     1321 tgctgggatt ttggcggaga accacaggta aagaaaaagg ccacattagc ggcctttttc
     1381 ggagaatatg ctcagtgcgg aatttcggag acttctttaa cgtcggtttt attgatctgc
     1441 tgtttgacac cattcgcgtc tgtatagcca agcaggcctg agtctgattc tttaggtttg
     1501 ccgtcgctga caatagtgcg accgtcattg gtgtgtatgg catagcttgt gcgcgtgcag
     1561 ccggtgaggg ctgaaagcgc aactgccgct gcgaacactg aaacaaaaat ctttttcaaa
     1621 gccagctcct tttatccata tcagttttgg tcactaacca tctaactata ggttattttt
     1681 tatgtgaaaa gaggcttgat ggtgggctgt atatcgagtt aggcgcgagg tgctatggtc
     1741 aaagtgtggt gtcaagcggg gaggaggctt atatgagatg taatccagca gatacatata
     1801 ccaagatgat ttaagtattc ctctttgtga ggttggatgt ctaatttata aaggatcttc
     1861 ttgagatcct tttttttcgc gcgtaatctc ttgccctgta aacgaaaaaa ccaccctggc
     1921 aggtggtttt tcgaaggtta ggtaatcctg gcagatcccc taaccgtggt aacagtcttg
     1981 tgcgagacat gtcaccaaat ttgtcctttc agtgtagcct cactaaggcc gccacttcaa
     2041 gaactcttga tacatctctc gcacatcctg tttaccaatg gccgttgcca atggcgatta
     2101 gtcgtgtctt tccgggttgg actcaagttg atagttaccg gataaggcgc agcggtcgga
     2161 ctgaacgggg ggttcgtgca tacagtccat cctggagcga actgccttcc cggaactgag
     2221 tgtcaggcgt ggaatgagaa accgcggcca taacagcgga gtgacaccgg taaaccgaaa
     2281 ggcaggaatg cgggggagca cgagggagcc accaggggga aacgcctggt atctttaagc
     2341 cgcatcgggt ttcgccacca ctgatttgag cgtcagattt cgtgatgctt gtcagggggg
     2401 cggagcctat ggaaaaacgg ctttgccgtg gccttatgcc tttttcgcta agaaccttcc
     2461 tggcattctc caggaaattt ctgctctgta cgtaag
//
