LOCUS       NZ_CP041589            10967 bp    DNA     circular CON 11-FEB-2024
DEFINITION  Acinetobacter baumannii strain J9 plasmid pJ9-2, complete sequence.
ACCESSION   NZ_CP041589
VERSION     NZ_CP041589.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12158047
            Assembly: GCF_007107525.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 10967)
  AUTHORS   Hamidian,M., Ambrose,S.J., Nigro,S.J., Judd,L., Holt,K.E. and
            Hall,R.M.
  TITLE     Analysis of multiply antibiotic resistant Acinetobacter baumannii
            isolates recovered during an outbreak in a Sydney hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10967)
  AUTHORS   Hamidian,M., Ambrose,S.J., Nigro,S.J., Judd,L., Iredell,J.,
            Holt,K.E. and Hall,R.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2024) Ithree Institute, University of Technology
            Sydney, Ithree Institute, Building 4, 15 Broadway, University of
            Technology Sydney, Ultimo, NSW 2007, Australia
  REMARK    Protein update by submitter
REFERENCE   3  (bases 1 to 10967)
  AUTHORS   Hamidian,M., Ambrose,S.J., Nigro,S.J., Judd,L., Iredell,J.,
            Holt,K.E. and Hall,R.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUL-2019) Ithree Institute, University of Technology
            Sydney, Ithree Institute, Building 4, 15 Broadway, University of
            Technology Sydney, Ultimo, NSW 2007, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JAN-2018
            Assembly Method                   :: Unicycler v. Jan-2018
            Assembly Name                     :: J9-V1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/11/2024 09:16:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,755
            CDSs (total)                      :: 3,659
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 21 of 57
            Pseudo Genes (incomplete)         :: 31 of 57
            Pseudo Genes (internal stop)      :: 10 of 57
            Pseudo Genes (multiple problems)  :: 4 of 57
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP041589.1.
            Bacteria and source DNA available from Ruth Hall.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10967
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="J9"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="pJ9-2"
                     /geo_loc_name="Australia: Westmead, Sydney"
                     /lat_lon="33.80 S 150.98 E"
                     /collection_date="1999"
                     /collected_by="Westmead Hospital"
     gene            144..1094
                     /gene="repM"
                     /locus_tag="FK728_RS18060"
                     /old_locus_tag="FK728_p200001"
     CDS             144..1094
                     /gene="repM"
                     /locus_tag="FK728_RS18060"
                     /old_locus_tag="FK728_p200001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786597.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_001208778.1"
                     /translation="MRELVVKDNALINASYNLDLVEQRLILLAIVEARESGKGINANDP
                     LEVHAEGYINQFGVHRNTAYQALKDACNDLFARQFSYQKINERGNIENYRSRWVSEIGY
                     VDNEAVVKLIFAPAIVPLITRLEEHFTKYELQQVSNLSSAYAVRLYELLIAWRSTGSTP
                     VIELSDFRQRIGVLDTEYKRMERFKTSVLELAIKQINEHTDITVKYEQHKRGRSISGFS
                     FTFKQKKKDNPPIERDPNTLDLFTKMTDAQRHMFANKLSELPEMGRYSQGTESYPQFAV
                     RIAEMLQDPDRIKELYPYLKKVGYMPSNKKDTVNG"
                     /name="repM"
     gene            1087..1662
                     /locus_tag="FK728_RS18065"
                     /old_locus_tag="FK728_p200002"
     CDS             1087..1662
                     /locus_tag="FK728_RS18065"
                     /old_locus_tag="FK728_p200002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005102333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_001096619.1"
                     /translation="MAKLSLSEVSKKFHVDRSTIYRAVRNGRLSRSSDGQFDLAEVIRC
                     FGEPEQTSQKIESSRQEGDESTKKLIAHLENEVKKYQEREERLMQQIDRMQTLIELKSV
                     APATAAPHQDATACDTKMPQHATTQQDTDNKKNNELNIAENVAVPQQETTAYHTQTLQH
                     ATLQSVAVPQHKKRGLFGRVLNAVFDND"
                     /name="plasmid replication DNA-binding protein"
     gene            1682..1825
                     /locus_tag="FK728_RS19015"
                     /old_locus_tag="FK728_p200003"
     CDS             1682..1825
                     /locus_tag="FK728_RS19015"
                     /old_locus_tag="FK728_p200003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001125246.1"
                     /translation="MPKLKDIALGIIVAPLLIPIMLIASYQDKKALKKELDECQKEKDQ
                     AS"
                     /name="hypothetical protein"
     gene            complement(2014..2532)
                     /locus_tag="FK728_RS18070"
                     /old_locus_tag="FK728_p200004"
     CDS             complement(2014..2532)
                     /locus_tag="FK728_RS18070"
                     /old_locus_tag="FK728_p200004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447193.1"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="WP_000447193.1"
                     /translation="METFAEPVFSNDLLAKAENGDTSAQLELAEIYLYGHGVDSDENQA
                     EVWALKSAENGNVAAMFWLADGYVTYARLMEDDDKNDSLEHFQKAFKWFQKASENGHSE
                     SMVELADLYTRADSGIEVNINKAFELREKAAKLGNKKAMRSLSVMYRDGIGIPKNTDLA
                     QSWWDKSEN"
                     /name="tetratricopeptide repeat protein"
     gene            2905..3192
                     /locus_tag="FK728_RS18075"
                     /old_locus_tag="FK728_p200005"
     CDS             2905..3192
                     /locus_tag="FK728_RS18075"
                     /old_locus_tag="FK728_p200005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_000438825.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTDG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNKYEHG"
                     /name="BrnT family toxin"
     gene            3179..3493
                     /locus_tag="FK728_RS18080"
                     /old_locus_tag="FK728_p200006"
     CDS             3179..3493
                     /locus_tag="FK728_RS18080"
                     /old_locus_tag="FK728_p200006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_061855757.1"
                     /translation="MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            3621..6032
                     /gene="znuD2"
                     /locus_tag="FK728_RS18085"
                     /old_locus_tag="FK728_p200007"
     CDS             3621..6032
                     /gene="znuD2"
                     /locus_tag="FK728_RS18085"
                     /old_locus_tag="FK728_p200007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor ZnuD2"
                     /protein_id="WP_000932953.1"
                     /translation="MLNKSKLFLALITLGASKILLAAEGPVTTLNTIVLTAQSDELGSE
                     LLGKSLNVSNQFIDTSKLKQRSTTLGDALGTELGIHSNQYGGGASTPIIRGQEGKRIKV
                     LQNNADVLDMSNMSPDHAVTVEPSLAKSIEIIRGASTLLYSSNSAAGVVNVIDYKIPTQ
                     MPQDGLEGNTTLRFNTGSNEKLTTAGVTVGLSPHVALRAEGLYRNAGNYKTPHYQSSSY
                     NSLEDLENQNSIYKNLKYLPESWAESRVGTLGLSWIDDNTYLGVSYTHRHDEYGLPAHS
                     HLYEGCGASAIGIDSRISGLKNYLLYYPQLMDEQDINYINPRPDCHQHNHIHETNFSHN
                     APYIDLNTRRYDVRGEFTQPFTGIDKIRTSLSYIDYFHNELEGDKITNFFKNTGKVGRI
                     ELSHQPLGELTGILGLQYLEQDNSALSPVHSQEGHTTYLDNQQLLNRNVTKNFSVFGLE
                     KYNWNDFTFELGARIEKQKVSMDYDIEKIKDSMKPWPNKYNSPYVEKNNKIRAQNLKSI
                     LEAVQPNKETAFSYAGTVHWRFAPNYILSLTGTHQERLPNAQEMYTHGMHLATNSFEIG
                     NRFLRKEKSNNLEISLAYKDDLLDYQISTYYYDFDNYIYLQTLNEVLGTTKVRDQHTLR
                     INHYSQSAANFYGLEGNIGYQFNSVYHGSLFGDYVKGRLTNLPDAVIAYDIWNREPTLA
                     PQKDRYTPRLPPARLGTRLKADFDESLKGEIEYYRVFKQDNISKFEQVTSGYNMLNMTL
                     AYKNKLSHTEYDLFFKANNLLDQKVYAHETFLPYIPQIGRNFSLGLNLNF"
                     /name="znuD2"
     gene            6337..6798
                     /locus_tag="FK728_RS18090"
                     /old_locus_tag="FK728_p200008"
     CDS             6337..6798
                     /locus_tag="FK728_RS18090"
                     /old_locus_tag="FK728_p200008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="WP_000761346.1"
                     /translation="MKLAEALLLRSDQQKKIISLKQRINANVLVQDGDQPSEDPNELLK
                     QVFSLIQEFQKLSYAIHETNALTKLNDGRSLLALLTLRDEFVEQHKTLTAAISNTSRES
                     DRYSTREIKWHKVIPVSSLQKQADDISLKLRDLNVLIQSTNWKIDLIEA"
                     /name="DIP1984 family protein"
     gene            complement(7234..8202)
                     /locus_tag="FK728_RS18095"
                     /old_locus_tag="FK728_p200009"
     CDS             complement(7234..8202)
                     /locus_tag="FK728_RS18095"
                     /old_locus_tag="FK728_p200009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611607.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="WP_012308653.1"
                     /translation="MLESRKEGFSARKFAELIKRHPSTIYRELKRNSINDVYQAQYASD
                     NTFARRRRGHRKLKIDSILWKFIVEAIRCLWSPQQIAKRLKTFPDLDQTMNVSHTTIYS
                     TIRALPKGELKKDLLSCLRHENKKRKANGEPKKDSILQDIKTIHERPAEVQERKIPGHW
                     EADLIKGKDNKSSIATLIERNTRLCILATLPDAKAESVRKALTEALKYLPAELRKTLTY
                     DRGREMSEHKILEEDLGIDVYFCDPHSPWQKGTCENMNGLIRQYLPKGIDLNQADQHYL
                     NQVAMSLNTRPRKALDWLTPLEKFAQLVDYHMAFETVAPHV"
                     /name="IS30 family transposase"
     gene            complement(8304..8636)
                     /locus_tag="FK728_RS18100"
                     /old_locus_tag="FK728_p200010"
     CDS             complement(8304..8636)
                     /locus_tag="FK728_RS18100"
                     /old_locus_tag="FK728_p200010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000390243.1"
                     /translation="MEDSTLNELIRNTELFFEHHWNVDLLGKPPEWSPVHFNFGKIPNY
                     DKQGVYAFIKGDLVTYIGVGTSRGAGRYRGNGLSARVMKYCRWNEDKTEYIAIDPRLKD
                     AGSMAE"
                     /name="hypothetical protein"
     gene            complement(8654..8920)
                     /locus_tag="FK728_RS18105"
                     /old_locus_tag="FK728_p200011"
     CDS             complement(8654..8920)
                     /locus_tag="FK728_RS18105"
                     /old_locus_tag="FK728_p200011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050441450.1"
                     /translation="MSVTETLDSKIKAQEEKLKQLKAQRQAALARERTKEKEQARKDDT
                     RRKILIGSCMLKITEDDEQARAKLIAQMDKYLTDERDRKLFNL"
                     /name="hypothetical protein"
     gene            9130..10566
                     /gene="mobQ"
                     /locus_tag="FK728_RS18110"
                     /old_locus_tag="FK728_p200012"
     CDS             9130..10566
                     /gene="mobQ"
                     /locus_tag="FK728_RS18110"
                     /old_locus_tag="FK728_p200012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005248576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029315.1"
                     /translation="MAIYHFSVKTVARSAGRSATAAIAYRAGEKIYCEREGREHDYSRK
                     TGVEYKEIYLPEGAPEHLKNREKLWNEVEQRETRKNSTVAREFEIAFPSELNQEQRLAM
                     LEELCASIVERHQVAVDACIHAPHTGSGSDERNYHAHILMSTRKLTPEGFTEKTRELDQ
                     KHSGEIDHWREHFADICNVHLNLAGSTARVDHRSYKDQENGLEATLHEGPKVTELRRRG
                     IETEISRSNDEIKQRNQAQLQYDKNMDVLIAENEIKLSTLKTEQQIQIKNSAKTPPIDE
                     KSLFEEKQRGTLGKVLKREISAKDANLDLDFMQRNLKQAEINLTKHHKHQNEFNQQLAQ
                     EIVKSGLKQSHDKLQSLVDQHNELTQNKPLLFGKKAWEAQRDEIYQEHKKLKGQHEHQK
                     KHGVKDLLENEKFKEHAWKKYQKQHPEKAKQYQTLYQSYKIVKKCVDEIKAEQQMKIRQ
                     EQQLKAQQHAPKMKSRGMSR"
                     /name="mobQ"
     Typing          145..1094
                     /name="rep_cluster_1172"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          9131..10563
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.213"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atatgacacc gtttaccttg caatatgaca ccgtttacct tgcaatatga caccgtttac
       61 cttgcaatat gacaccgttt accttgctta tagcactaaa attaattatt gtgtcatagc
      121 ataataatta atactggtgc ttcatgagag aattagtggt aaaagacaat gccttaatca
      181 atgcaagcta taacttagat ttagtagaac aacgtttaat tttattggcc attgttgagg
      241 caagagaaag cgggaaaggt attaatgcga atgatcccct tgaagtacat gcagaaggct
      301 atatcaatca atttggcgta catcgcaata cggcttatca agcattaaaa gatgcctgta
      361 atgatttatt tgcaagacaa tttagctatc aaaaaataaa tgaacgaggg aatattgaga
      421 actatagatc ccgttgggtt agtgaaattg gatatgtaga taatgaagca gtggttaaac
      481 ttatctttgc cccagccata gttcccttaa ttacacgctt agaagagcat ttcactaaat
      541 acgaattgca gcaagttagt aatctcagca gtgcttatgc tgttcgctta tatgaattat
      601 taattgcttg gagaagtact ggctctactc ctgttataga gctaagtgat ttccgtcaaa
      661 gaattggcgt actcgataca gagtacaagc gtatggaacg ctttaaaact agtgtacttg
      721 agcttgctat taaacaaatt aacgaacata cagatatcac tgtgaagtat gagcaacaca
      781 aaagaggtcg atcaatttca ggattctctt ttacttttaa acagaaaaag aaggacaacc
      841 caccgataga aagagatccg aatacgttag atctctttac aaagatgact gatgcacaac
      901 gccatatgtt tgcaaataaa ctttctgaac ttcctgaaat gggtcgttat tcccagggaa
      961 ctgaaagcta tccgcaattt gctgttcgta ttgctgagat gctgcaagac cctgaccgaa
     1021 taaaagaact atacccatac ctaaaaaaag tgggatatat gccatcaaat aaaaaggaca
     1081 ccgtaaatgg ctaagttatc actaagtgaa gtatctaaaa aattccatgt ggatagatca
     1141 accatttaca gagctgtacg taatggacgt ttatcacgct ccagtgatgg acaattcgat
     1201 ctagcagagg tcatccgatg ctttggagaa cctgagcaaa catctcaaaa aattgaatca
     1261 tctaggcaag aaggtgatga atctacaaaa aaacttattg cccatttaga aaatgaagtc
     1321 aaaaaatacc aagaacgtga agaacggtta atgcaacaaa ttgaccgtat gcaaacactc
     1381 attgagctga aaagtgttgc acctgccaca gcagcaccac accaagatgc tacggcatgc
     1441 gacaccaaga tgccacagca tgcgacaaca caacaagaca ctgataacaa aaagaataat
     1501 gaattaaata ttgcagaaaa tgtagcagtg ccacagcaag aaactacggc ataccacacc
     1561 caaacgctac agcatgccac gttgcaaagt gtggcagtgc cacaacacaa aaaacgtggt
     1621 ttatttggcc gtgtgctgaa tgccgtcttt gataatgact gagcaataag ggtgaaagat
     1681 catgccgaaa ctgaaagaca ttgccctggg aattattgta gctccgctgc tgatcccgat
     1741 catgctgatt gcatcgtacc aggataaaaa ggcgctgaag aaagagctgg atgaatgcca
     1801 aaaagaaaaa gaccaggcat cctgattatt caattgagct aatcaaaagt gtggaacatt
     1861 gatgaaataa tagcgtggaa cgtgaaaagt cataaaaaaa gcccgaacag ggatgctcgg
     1921 gctataaact agaaactaca gcgttgaatt ataaggataa ttatacagca tttcgtataa
     1981 ggtgtattat gttaatttta ggaaatatta aaactaattt tcagacttat cccaccaact
     2041 ttgagctaaa tcagtatttt taggaatacc tataccatca cgatacataa cggaaagact
     2101 tcgcattgct ttcttattac ctaactttgc agccttttca cgaagttcaa aagctttatt
     2161 aatattaact tctattccgc tatctgcacg agtatataga tcagctaact caaccattga
     2221 ttcagaatgg ccattttctg aggctttctg aaaccactta aaagcttttt ggaaatgttc
     2281 taaagaatcg tttttgtcat catcttccat taatctagca taagtaacat atccatcagc
     2341 taaccaaaac attgctgcta catttccatt ttctgctgat ttaagagccc aaacttcagc
     2401 ttgattttca tctgaatcaa caccatgacc atatagataa atctcggcta attctaactg
     2461 agcagaagta tctccatttt ctgctttcgc taataaatca ttagaaaaaa caggttctgc
     2521 aaatgtttcc attgaaaggc aaactcctaa agccataaaa atatttgtta atttattcaa
     2581 tttttaacct ttaaactaaa ctgttaggct tgtatatttt tatatcatac ctaaaagaaa
     2641 actctaaaat tagagtatgt cttatctcta tttaacataa tggcgattat acgaagtaca
     2701 cttgtatatt aatataaata tcaactactt aataaaaata gaaaaatccc aaaatgccca
     2761 aaaagccgac tttgaaacaa gtcggctttt ttatgagcag ttttgatact tcttgccaat
     2821 aattttgact aaaaattaca cgaatctggt tgagtgatac aattttgtga tacattattg
     2881 tatatacaaa tttgaatgtt ttttatggaa cagtattttg aatgggatga ggctaagaat
     2941 cgaaagaatc agaaaaagca tgatatctct ttcgaaactg caagccttgt ttttgaagat
     3001 ccattacgga tctcaatcca agacagacat accgatggtg aagaacgttg gcaaaccatt
     3061 ggacgagtta aaggcgtact aatgttattg gtggctcaca ccatttttga tgaagatgac
     3121 tgtgaaatca tacgaatcat tagtgcaagg caagtgacta aagcggagcg aaacaaatat
     3181 gagcatggtt agatactcac gcaaagagct gaatgagaaa tttggtgaga agcaagatgc
     3241 tgaaattcaa cgcttgcttg ctaaaggcac ggttcctgat gatcagctag atctatcaga
     3301 tattcctgaa attacagact ggagcaatgc tgtacgccaa aatcaatttt accgcccagt
     3361 aaagcaacaa acttcaattc ggttagatgc tgatgtactt gcttggttca aagcacaagg
     3421 caaaggttat caaacacgta tgaatgaaat tttacgagat gctatgctaa aagaattaaa
     3481 aaatcatcaa taagaatggg agcttaggct cccatttttg acttcatttt taggggggat
     3541 taccattcaa aaatttaaat agtagatttt ataaattttt aagtgttata ttataatata
     3601 acattaaatt tggttgtttt atgctaaata aaagtaaact atttttagct ttaattactt
     3661 taggggcctc gaaaatttta ttggcagctg aagggcctgt tactacctta aatacaatag
     3721 tattaactgc acaatctgat gagttagggt ctgagctgtt aggcaaaagt ctaaatgtta
     3781 gtaatcagtt tattgatact agtaaactta aacagcgttc gacaactcta ggggatgcct
     3841 tgggtacgga attaggaatt cattctaatc aatatggagg tggagcatct actcctatta
     3901 ttcgtgggca ggaaggaaaa cgtattaaag ttttacagaa taatgctgac gtgttggata
     3961 tgtctaatat gtccccagat cacgctgtca cagttgaacc aagtttagct aagtctattg
     4021 aaattatacg tggtgcaagt acccttttgt atagttcaaa tagtgcggca ggggtagtca
     4081 atgtcattga ctataaaatt ccaacccaaa tgcctcaaga cggattagag ggtaatacta
     4141 cattacgttt taatactggc agtaatgaga agttgaccac agcaggtgta acagttggct
     4201 taagcccaca tgtagcttta cgggcagaag ggttatatcg taatgctggt aactataaaa
     4261 ctcctcatta ccagtctagc tcttataatt ctttagaaga tcttgaaaac caaaacagta
     4321 tttataaaaa tttaaaatat ttaccagaaa gctgggcaga gtcgagagta ggtacgcttg
     4381 gattatcctg gatagacgat aatacttatt taggagtttc ttatacacat cgtcatgatg
     4441 aatatggttt accagcccat agccatttat atgaaggttg tggtgcctct gcaatcggta
     4501 tcgattcacg aattagtgga ttaaaaaatt atcttttata ttatcctcaa ttgatggatg
     4561 agcaagatat taattacata aatccacgtc ctgattgtca tcagcataat catattcatg
     4621 aaactaattt ctctcataat gctccttata ttgatttaaa tactcgacgt tatgatgtgc
     4681 gtggagaatt cactcaacca tttaccggta ttgacaaaat tcgtacctca ttgagttata
     4741 tagattattt tcacaatgaa ctcgagggag ataagattac taactttttt aaaaatacag
     4801 gaaaagtagg ccgcatagag ttatcacatc agccattagg ggaattaacc ggtatcttgg
     4861 ggctacaata tttggaacag gataatagtg ctttatcacc agtccattct caagaaggcc
     4921 ataccaccta tctagataat cagcagctat taaatcgaaa tgtcactaaa aactttagtg
     4981 tatttggcct agaaaaatat aactggaacg actttacttt cgaactaggt gctcgtatag
     5041 aaaagcagaa agttagtatg gattatgata tagaaaaaat taaagattca atgaaacctt
     5101 ggcctaataa atataacagt ccttatgtag aaaaaaataa taaaatcagg gctcaaaatt
     5161 taaaatctat tttagaagct gttcaaccta acaaagaaac agctttttct tatgcaggta
     5221 cagtccactg gagatttgct ccaaattata tcttatcatt aacaggtaca catcaagaaa
     5281 gattacctaa tgcacaagaa atgtatactc atggtatgca tctcgctact aattcttttg
     5341 aaataggtaa ccgctttcta agaaaagaga aatccaataa tttagaaatc agtctggcct
     5401 ataaggatga tttacttgat tatcaaatca gtacttacta ttatgatttt gataactata
     5461 tatatttaca gacactcaat gaagttttag gaactacaaa agttagagat cagcatacac
     5521 tacgtattaa tcattatagc cagtctgctg ctaattttta tggattagag ggcaacatag
     5581 ggtatcaatt taattctgtt tatcacggct ctctttttgg agattatgtg aaaggccgtt
     5641 taacaaattt acctgatgct gtaattgcat acgatatctg gaatagagaa ccgactttag
     5701 ctccccaaaa ggatcgttac actcctagat tacctcctgc tcgtttgggt acacgactaa
     5761 aagctgattt tgatgaatcg cttaaaggtg aaatagaata ttatcgagtc tttaaacaag
     5821 ataatatttc taaatttgag caggttacat caggttataa tatgcttaat atgactttag
     5881 catataaaaa taagctatct cacacagaat atgatttatt ctttaaagcg aataatttac
     5941 ttgatcagaa agtctatgcc catgaaacat ttttgccata tattccccaa attggaagaa
     6001 attttagttt aggcttaaat cttaatttct agataagata ctgtaatctt ctaagaaaat
     6061 ttcttagaag atttttctta taaaatgatt agattaataa ttaaggtaag cgtccgctga
     6121 atcccatacc tattttttaa ttcgatttag gtttccagca gtgtggcagt aactcttcaa
     6181 tctgggtcac tttatgtgtc ggcagccttt tcagcactga atagccaccg attttgtaga
     6241 caccttttag ctagataaaa agcaattttt ttgatcttta aagaatgagg atataagctg
     6301 tttaaaactt aattctaatt ggttaatagg tgaaaaatga aattagcaga agctctctta
     6361 cttcgaagtg atcaacaaaa aaaaattatt tcactaaaac aacgtatcaa tgcaaatgta
     6421 ttggtacaag atggtgatca accatctgaa gatccaaatg aactactaaa acaagtattt
     6481 tctttaattc aagaatttca aaaattaagt tatgcaattc atgaaactaa tgctttaaca
     6541 aaattaaatg atggccgttc tctacttgct ttattgactt tgcgtgatga atttgtagaa
     6601 cagcacaaaa ccctaactgc agcaataagt aatacatctc gtgaatctga tcgttatagt
     6661 acgcgggaaa taaagtggca taaagttatt ccagtttcat cattacaaaa acaagctgat
     6721 gatattagtc ttaaattacg tgatttaaac gtgttaattc agtcgactaa ttggaaaatt
     6781 gatttgattg aagcctaata gtcctttaga tataatcaag ttgaacatac tgggcgagta
     6841 caagatccct tacttccaca acataggatt gtaaataaag tgtagtggct tgcaaagcaa
     6901 gtggataagc ttaaaatttt aagtacaaat caatccatca cttggcataa ctcaaatctt
     6961 tgtgcaaaca aagttacacg ttacttataa ctaccatgta ctgacagtat gttcacctca
     7021 attactaaaa ataaagaaat gaacaagcat cagctcaact acatttctat ttttaattca
     7081 gttcggcatt taacataaaa tctcttatgc gaaattacat accaacttta accacattgt
     7141 ggacaggttg gagtttagag ataagaaaaa tttctagagc atttgctaaa taagccctat
     7201 cacgttcaaa gcctatcgta atgatgggcg aattcaaaca tgaggtgcga cagtttcaaa
     7261 agccatatga taatcaacaa gctgagcaaa tttctctaat ggtgtaagcc aatctaacgc
     7321 ctttctagga cgagtattca gtgacatggc aacttgattt aaataatgct gatctgcctg
     7381 atttaaatca atccctttag gtaaatattg cctaattaaa ccattcatat tttcgcatgt
     7441 gcctttttgc cagggtgaat gtgggtcaca gaaatataca tctatgccta aatcttcttc
     7501 gagtatttta tgttctgaca tctcacgtcc acggtcatag gtcaacgttt tacgcagttc
     7561 tgcaggtaaa tatttcagag cttcagttaa agccttgcgc actgattctg cctttgcatc
     7621 aggtaatgtt gccaagatac agagccgtgt atttcgttca ataagtgttg ctatcgaact
     7681 tttattgtct ttacctttaa ttaaatcagc ttcccaatga cccggtattt ttctttcttg
     7741 aacttcggct gggcgctcat gaatagtttt aatatcctgt aatatagaat cttttttagg
     7801 ttcaccgtta gcttttcgct ttttattttc atgacgtaga caggataata agtctttttt
     7861 caactcaccc ttgggtaatg ctcgtatcgt tgaataaatc gttgtatggc ttacattcat
     7921 tgtttgatcc aaatcaggaa atgtctttaa acgctttgct atttgctgag gagaccataa
     7981 acaacggatc gcttcaacaa taaatttcca gaggattgaa tcgattttga gttttctgtg
     8041 accacgtcta cgtctagcaa aagtgttatc agaagcatat tgagcttgat aaacgtcatt
     8101 gatgctattt cttttaagct cacgatagat cgtactagga tgtcttttaa taagttcagc
     8161 aaattttctg gctgaaaagc cttcttttct tgactcaagc attaatgcag tacgatcttc
     8221 aaagttaaga tgatggtatg acaattttat atactccata aaccctttaa attaattagg
     8281 tggtttatgt cgcacttcaa gttttactct gccatggatc cagcatcttt taatcttgga
     8341 tctatcgcaa tatattcagt tttatcttca ttccatcggc aatatttcat taccctggcg
     8401 cttaagccat taccacgata acggccagca cctctagaag tgccaacacc gatataagtc
     8461 actaaatccc ctttgataaa cgcatataca ccttgtttat cgtaattagg aattttccca
     8521 aaattaaaat gtactggtga ccattcagga ggctttccta atagatctac attccagtga
     8581 tgttcaaaga ataattctgt attacgtatt aactcattaa gtgttgaatc ttccactact
     8641 catctctcaa aatttataga ttaaatagct ttcggtcacg ctcatccgtt aaatacttat
     8701 ccatctgagc aattagttta gctctggctt gttcatcatc ttcagtgatt tttaacatac
     8761 aggatccaat taaaatcttg cgtctggtat cgtcctttcg tgcctgttct ttctctttgg
     8821 tacgctctct tgccagtgct gcctgtcttt gagcctttaa ctgctttagc ttttcttcct
     8881 gtgccttgat tttgctgtct agtgtttcag taacgctcat taatccaata ccatcattct
     8941 gggaaaccat ccctaacata agcgcacttg aatatgcatt caaggtgcta tatgtatagt
     9001 aatgttttcc tcgaagagcg cacttatgca aactatgttt gcaagtccga ttggggtctc
     9061 cccaataccc cgacaaccgt attcacggtt gtatttcact acgtgaaaat ttaaaagcga
     9121 aaaatagaca tggcgattta ccacttttca gtcaaaaccg tagcacgatc agcagggcgt
     9181 tccgccactg ctgcgattgc ctatcgggca ggtgaaaaga tctattgtga acgtgaaggc
     9241 cgagaacatg actacagccg aaaaaccggt gtggaataca aagaaattta tttaccagaa
     9301 ggcgcaccag agcatttaaa gaatcgagaa aaactctgga acgaagttga acaacgggag
     9361 acccgaaaaa actcgactgt agccagggaa tttgaaattg cctttccaag tgaactgaac
     9421 caggaacaac gtctggccat gctcgaagaa ttatgtgcca gcatcgtgga aagacatcaa
     9481 gtagcagtcg atgcctgtat tcatgcaccg cataccggat ccggcagtga tgagcgcaat
     9541 tatcacgccc atatcctaat gagcacacgt aaacttacgc cagaaggttt tacagagaaa
     9601 acccgggaac tcgatcagaa acacagtgga gaaatcgatc actggcgtga acactttgcc
     9661 gatatctgca atgtgcacct gaatctggcc ggatccacag cccgggtcga ccaccgcagc
     9721 tacaaagacc aggagaatgg cctggaagcc accctgcacg aaggaccgaa agtcaccgaa
     9781 ctacgtagaa gaggcatcga aaccgaaatc agccgaagca atgatgaaat caaacagaga
     9841 aatcaggctc agttgcaata cgacaaaaat atggacgttc taattgctga aaatgagata
     9901 aaactcagca cactgaaaac agaacagcag atccaaatca agaatagcgc taaaacgcca
     9961 ccaattgacg aaaaatccct gtttgaggaa aaacagaggg gaacccttgg caaagtgcta
    10021 aaacgcgaaa tcagcgcaaa agacgcgaat ctagacctgg attttatgca gcgcaatctc
    10081 aaacaagccg aaataaacct gaccaagcac cacaaacatc aaaacgagtt caatcagcag
    10141 cttgcccagg agatcgtgaa aagcggactc aagcaaagtc atgacaaatt gcaaagcctg
    10201 gtcgatcaac acaacgaatt aacccagaac aaacccttac tgtttggaaa aaaagcctgg
    10261 gaagcccaac gtgatgagat ctaccaggaa cacaaaaagt tgaaaggtca gcatgaacac
    10321 cagaaaaaac atggtgtgaa ggatttattg gagaatgaaa agttcaaaga acatgcctgg
    10381 aagaaatatc aaaaacagca tccggagaaa gccaagcaat accagaccct ctaccagtcc
    10441 tacaagatcg ttaaaaaatg tgtggatgag attaaagcag aacaacagat gaaaatcaga
    10501 caagaacaac aactcaaagc acagcaacat gcgcctaaaa tgaaatctcg tggcatgagt
    10561 cgttaaacag agcgagtgtc tacgagcgaa ctgagaaaat ttgcccatcc ctttccctga
    10621 ttacaagctc cctatccatc aatcacctgc ataccagagc atcccgcatg ggtgcgaact
    10681 ttccaagttt gattcctaaa tcgagcgtag cgagaaaaaa agctcatgag cgaagcgaat
    10741 tccgagttgc ttttgatctt gcttttgctt tttctcaaat tcacgaaaat atgaaccaaa
    10801 aattgccccg acgaatcgag cgaaagcgag attcaataga gtttgagcga acgaagtgaa
    10861 gcgaaaacca agggcaattt ttcactcctt gggcttttaa ttattttaaa gtttttaaat
    10921 gcttttagat atgctgaaag cctttgttca caatgctttt aaggttt
//
