LOCUS       NZ_CP041418             3214 bp    DNA     circular CON 23-JUN-2024
DEFINITION  Escherichia coli strain STEC711 plasmid pSTEC711_2, complete
            sequence.
ACCESSION   NZ_CP041418
VERSION     NZ_CP041418.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12214770
            Assembly: GCF_009949145.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Yang,X., Bai,X., Zhang,J., Sun,H., Fu,S., Fan,R., He,X., Scheutz,F.,
            Matussek,A. and Xiong,Y.
  TITLE     Escherichia coli strains producing a novel Shiga toxin 2 subtype
            circulate in China
  JOURNAL   Int J Med Microbiol 310 (1), 151377 (2020)
   PUBMED   31757694
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Xi,Y., Bai,X., Zhang,J. and Xiong,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2019) mEpiLab, Infectious Disease Research Centre,
            Hopkirk Research Institute, IVABS, Massey University, Private Bag
            11-222, Palmerston North 4442, New Zealand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 127x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009949145.1-RS_2024_06_23
            Annotation Date                   :: 06/23/2024 05:09:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,099
            CDSs (total)                      :: 4,975
            Genes (coding)                    :: 4,804
            CDSs (with protein)               :: 4,804
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 171
            CDSs (without protein)            :: 171
            Pseudo Genes (ambiguous residues) :: 0 of 171
            Pseudo Genes (frameshifted)       :: 78 of 171
            Pseudo Genes (incomplete)         :: 104 of 171
            Pseudo Genes (internal stop)      :: 39 of 171
            Pseudo Genes (multiple problems)  :: 46 of 171
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP041418.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="STEC711"
                     /isolation_source="goat"
                     /db_xref="taxon:562"
                     /plasmid="pSTEC711_2"
                     /geo_loc_name="China: Shandong"
                     /collection_date="2017-11-08"
                     /collected_by="State Key Laboratory of Infectious Disease
                     Prevention and Control, National Institute for Communicable
                     Disease Control and Prevention, Chinese Center for Disease
                     Control and Prevention"
     gene            join(3144..3214,1..163)
                     /locus_tag="FNE87_RS25800"
     CDS             join(3144..3214,1..163)
                     /locus_tag="FNE87_RS25800"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_250696867.1"
                     /translation="MRFPRLAGCTPERGGKHGELRDNAADEAMGKLRELLPEKRRKDAV
                     LCVEYVMTASPEWWDKLHHGSRRSFSPVLSNG"
                     /name="plasmid recombination protein"
     gene            303..749
                     /locus_tag="FNE87_RS25805"
     CDS             303..749
                     /locus_tag="FNE87_RS25805"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_250696868.1"
                     /translation="MRDDQSTYAESVKKLGLERGIEGSRATHQTVQHYYESINRGTRSQ
                     VSISPEALEPRVLRKGIFTKDVEDQAAIAKRLSQAVNDGFAGTIAMASQSAQNAKRARD
                     LQKTMDSQQNAYRALQSLLKDFPGSRWTHILTMAQTFQQQKSRS"
                     /name="plasmid recombination protein"
     gene            complement(867..1739)
                     /locus_tag="FNE87_RS24305"
                     /old_locus_tag="FNE87_24715"
     CDS             complement(867..1739)
                     /locus_tag="FNE87_RS24305"
                     /old_locus_tag="FNE87_24715"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_250696869.1"
                     /translation="MLYWKKMPSLWIGNRLTEFNSLETSHAIAAIKIYITFCLFGKEED
                     DGVRTVKLTFTEISKIASISRSLVNEGLKLLDNKKLIKYLSPASKRKKIYTVDVDGYIH
                     DGWCKLPMAGIVSEDRTISAFQSMHNRYHFELVALQLYLYLLYARDNFQEHVLARKQTI
                     CEKLACRNVDLNKAITYLIHIGLLKGIKQKAVESYPIELFHDSFYFYVKTGASNALTFK
                     KQPLVNPPEKKFRTDIPEGELLLALMIYSKVVIQYFFTPLFITCFARDNPRFQTFITRY
                     STRIHSLKS"
                     /name="hypothetical protein"
     gene            complement(2085..2606)
                     /locus_tag="FNE87_RS24310"
                     /old_locus_tag="FNE87_24720"
     CDS             complement(2085..2606)
                     /locus_tag="FNE87_RS24310"
                     /old_locus_tag="FNE87_24720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010657475.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_099368844.1"
                     /translation="MDNSQIGTPESHEQDNYNFVQVSRAYLKEWRALTRKSPIASEILM
                     YLIENMGRTTNAVVCSYTTLMEITGVSRASVARAVKILKEDRWMQAVKIGNATAYCVNA
                     KAFWQAGRNQKKYAIFQATVIASSSEQESDFLEKSQQDLRYIPFVDLKDRPIVGTDELP
                     PPDQTDLDLD"
                     /name="replication/maintenance protein RepL"
ORIGIN
        1 ggcagatgag gcgatgggaa agctgcggga gctgttgcct gaaaaacgcc gtaaagatgc
       61 ggtgttgtgc gtcgagtacg tcatgacggc aagccctgag tggtgggaca agctacacca
      121 cggcagcagg cggagttttt cgcccgttct gagcaatggc tgagaacaaa tacggaaaga
      181 tcgcgttgtg gcggcggtgg ttcacaggga cgaggccacc ccgcacctgt ctgcgttcgt
      241 ggtgccattg acgcagggat gggcggctgt ccgcgaaaga gtttattggc gggcggtcaa
      301 agatgcgtga cgatcagagc acctacgctg aatcggtaaa aaaactgggt cttgagcgcg
      361 gcatcgaggg aagccgtgcc acgcatcaga cagttcagca ctactacgaa tcgataaacc
      421 gtggcacccg tagccaggta tcgatctcac cggaggcgct ggagccgcgt gtactgcgga
      481 agggtatttt caccaaagac gtggaagatc aggcagccat agccaaacgt ttaagccagg
      541 cagtgaacga tggctttgca ggaaccatcg ctatggcctc tcagagcgcc cagaatgcca
      601 aaagagcgcg cgatctacag aagactatgg attcgcagca aaacgcctac agagcgttac
      661 agagcctttt aaaggacttt cccgggagca gatggaccca tattctgacg atggcgcaga
      721 cgttccagca acagaaatca cgatcgtgaa aaacagcgcc agcttgaacg ggagcaggaa
      781 cgccagcaac gtcagaaaac tgacagaggt attagccgat gagtgaaaag cagcgccgtg
      841 aagggtttcg aaaagcggaa gcatcactaa gacttgaggg aatggatcct agtggagtac
      901 ctcgttatga atgtttgaaa acgaggatta tctctggcga aacaagttat gaacaagggc
      961 gtaaagaaat attggattac taccttagaa taaatcataa gggcgaggag taattcgccc
     1021 tcaggtatgt cagtacggaa tttcttttct ggagggttaa caagaggttg ctttttgaat
     1081 gtaagcgcat tgcttgctcc tgtttttaca taaaaataaa atgaatcatg aaaaagttct
     1141 atgggataag actccactgc cttttgttta attcctttta aaagacctat gtgtattaaa
     1201 tatgttattg ctttatttaa atctacattg cggcaagcaa gcttttcgca aatagtttgt
     1261 tttctagcta acacatgctc ctggaagtta tctctggcat atagaaggta taaatatagc
     1321 tgaagcgcta caagctcaaa atggtatcta ttatgcattg actggaaggc gctgattgtc
     1381 ctgtcttcgc taacgatgcc tgccataggc agcttacacc atccgtcatg aatgtaccca
     1441 tcaacatcca ctgtatagat tttctttctt tttgacgcgg gcgataggta tttaattagt
     1501 tttttattat cgagcaattt gagaccttca tttaccaaag atcggctgat agaagctatt
     1561 ttgcttattt ctgtaaacgt aagttttacg gttcgcactc catcatcctc ttcttttcca
     1621 aagagacaaa aagttatata tatctttatg gcggcaatgg catgtgaagt ctctaaacta
     1681 ttaaactctg tcagtctatt accgatccag agtgatggca tttttttcca atataacatt
     1741 agtgactcct ttatgcggaa atgaaagtga aataaatcat aattaacaca caaatggcca
     1801 atgcaagact agtaagtgac cctgccactt ttcccagcct ttcagcaagc tctacaaatc
     1861 gctctaagtc acccattttg acctctgctc ctgtttaccg atataccgat tatcccagta
     1921 atccgcgtat ctgtatctac aggagcaaaa gtctatctag aaaggtatgg gaaaaaatta
     1981 atataacttt ttataattaa atcaaaaagt taacatcaaa aaatggttat caaatgaact
     2041 tcaatatggt tcacggtgaa ccatatttga aggttttcag aggctcagtc gagatctaga
     2101 tctgtttgat ccggaggcgg gagttcatcg gtgccaacaa tagggcggtc tttgagatcg
     2161 acaaaaggaa tatatcgaag atcttgctgt gatttttcta agaaatcaga ctcttgctct
     2221 gaggaggagg caatcacggt agcttggaag attgcgtatt ttttttgatt tctacctgcc
     2281 tgccaaaatg ctttggcatt aacacaatat gctgtggcat tacctatttt tactgcctgc
     2341 atccatcgat cttccttcag gattttaacc gcccttgcta ctgatgctct agagaccccg
     2401 gttatctcca tcaaagtcgt atagctgcaa acaacagcat tagttgtcct gcccatattc
     2461 tcaatcaagt acataaggat ttcagaggca atagggcttt tccttgtcag cgcacgccat
     2521 tccttgagat acgcgcggct aacctggacg aaattatagt tatcctgctc atgagactct
     2581 ggagtgccaa tctgtgaatt atccattgct ctatagacca tttcaatatc ccatttgata
     2641 ccggttgtat cattagagat gcaacaggtc aacttgagtc tcatagctag tgagactcat
     2701 aagtctcatg gctggtgaga ctcccccttt ttttaaccca atataatcag cgatttactg
     2761 aaagctcctt ttctatatct attaagtccg tgcatacgta gcccaatgta cgcctagtgg
     2821 acattccgct tcctcgacat gctgttcact cctcgcttcg ctgcggtgcg cctcagcatg
     2881 tcttgcggtg agcggtattt ttttaaggac tgacactgga actaatttcg tcaagggcga
     2941 gcgtagcgag tgcatttcac ccttgacgac gcacaaataa aacaggcttg gcagtggtgg
     3001 gaggtggcgc ggagcgacgc cttccacctg ccagaccgcc gttgattttg accttctggc
     3061 agcttgctgc ccgaacgatt ttgcgaagca aagtctagtg agttacacta tcatcctgat
     3121 agtggcccgc cggaggcatc gcaatgcgat tcccccgact ggctggatgt acgccagaac
     3181 gagggggtaa gcatggcgaa ttacgcgata atgc
//
