LOCUS       NZ_CP041377            11970 bp    DNA     circular CON 09-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KP58 plasmid pKP58-4, complete
            sequence.
ACCESSION   NZ_CP041377
VERSION     NZ_CP041377.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12228410
            Assembly: GCF_008728695.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Ruan,Z. and He,F.
  TITLE     Emergence of a pandrug resistant hypervirulent Klebsiella pneumoniae
            isolate in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   Ruan,Z. and He,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2019) Department of Clinical Laboratory, Sir Run
            Run Shaw Hospital, School of Medicine, Zhejiang University, 3
            Qingchun East Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Oxford Nanopore GridION; Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/09/2024 01:54:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,965
            CDSs (total)                      :: 5,844
            Genes (coding)                    :: 5,660
            CDSs (with protein)               :: 5,660
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 67 of 184
            Pseudo Genes (incomplete)         :: 122 of 184
            Pseudo Genes (internal stop)      :: 27 of 184
            Pseudo Genes (multiple problems)  :: 32 of 184
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP041377.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP58"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP58-4"
                     /geo_loc_name="China: Hangzhou"
                     /collection_date="2018-05-04"
                     /collected_by="Fang He"
     gene            join(11960..11970,1..169)
                     /locus_tag="FM021_RS30040"
                     /old_locus_tag="FM021_30045"
     CDS             join(11960..11970,1..169)
                     /locus_tag="FM021_RS30040"
                     /old_locus_tag="FM021_30045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(196..726)
                     /locus_tag="FM021_RS30045"
                     /old_locus_tag="FM021_30050"
     CDS             complement(196..726)
                     /locus_tag="FM021_RS30045"
                     /old_locus_tag="FM021_30050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(733..1464)
                     /gene="mobC"
                     /locus_tag="FM021_RS30050"
                     /old_locus_tag="FM021_30055"
     CDS             complement(733..1464)
                     /gene="mobC"
                     /locus_tag="FM021_RS30050"
                     /old_locus_tag="FM021_30055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(1464..3428)
                     /locus_tag="FM021_RS30055"
                     /old_locus_tag="FM021_30060"
     CDS             complement(1464..3428)
                     /locus_tag="FM021_RS30055"
                     /old_locus_tag="FM021_30060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(4912..5061)
                     /locus_tag="FM021_RS30065"
                     /old_locus_tag="FM021_30070"
     CDS             complement(4912..5061)
                     /locus_tag="FM021_RS30065"
                     /old_locus_tag="FM021_30070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(5146..5403)
                     /locus_tag="FM021_RS30070"
                     /old_locus_tag="FM021_30075"
     CDS             complement(5146..5403)
                     /locus_tag="FM021_RS30070"
                     /old_locus_tag="FM021_30075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(5413..7098)
                     /locus_tag="FM021_RS30075"
                     /old_locus_tag="FM021_30080"
     CDS             complement(5413..7098)
                     /locus_tag="FM021_RS30075"
                     /old_locus_tag="FM021_30080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            complement(7203..8690)
                     /gene="ltrA"
                     /locus_tag="FM021_RS30080"
                     /old_locus_tag="FM021_30085"
     CDS             complement(7203..8690)
                     /gene="ltrA"
                     /locus_tag="FM021_RS30080"
                     /old_locus_tag="FM021_30085"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            9335..9580
                     /locus_tag="FM021_RS30090"
                     /old_locus_tag="FM021_30095"
     CDS             9335..9580
                     /locus_tag="FM021_RS30090"
                     /old_locus_tag="FM021_30095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            9854..10225
                     /gene="tolA"
                     /locus_tag="FM021_RS30095"
                     /old_locus_tag="FM021_30100"
     CDS             9854..10225
                     /gene="tolA"
                     /locus_tag="FM021_RS30095"
                     /old_locus_tag="FM021_30100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            10222..10587
                     /locus_tag="FM021_RS31135"
     CDS             10222..10587
                     /locus_tag="FM021_RS31135"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            10762..11040
                     /locus_tag="FM021_RS30105"
                     /old_locus_tag="FM021_30110"
     CDS             10762..11040
                     /locus_tag="FM021_RS30105"
                     /old_locus_tag="FM021_30110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            11028..11342
                     /locus_tag="FM021_RS30110"
                     /old_locus_tag="FM021_30115"
     CDS             11028..11342
                     /locus_tag="FM021_RS30110"
                     /old_locus_tag="FM021_30115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            11506..11934
                     /locus_tag="FM021_RS30115"
                     /old_locus_tag="FM021_30120"
     CDS             11506..11934
                     /locus_tag="FM021_RS30115"
                     /old_locus_tag="FM021_30120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     BGC             413..11970
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          4231..4809
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(737..1464)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 ggaaaaaact gccctcaata tggctaaatt cattaaggat cagtcgctgc tgttgctgga
       61 aaagctgaac gagctggaca tggatacccc ggccgacatg tgcgaacggc ttcacgagga
      121 tgcggaaaca ctctggcagg tgatgcggga taagctcggg gaagaataac agcaccggcc
      181 atgccggtgc tggcgttatt ttttgccgaa cggccaccac cggcgggcgg gtttgtcctc
      241 cggcagttca ggcaccggca cgttaaaata gcggctgtta tattcccgca tcagctccgc
      301 tttcagcttc tcgttcaccg cgctttcccc gtgccgctcg ctgaccacct caatcatcgc
      361 gttaatctgc tccggtgagc acttcccggc atcaaccacc tgcacaccgt tctcgatggc
      421 gaaccgctcc acctttcgat agaggtaatc acacagcttc attcctgtgg gtttgtcccg
      481 ttcccattgc ggattttcac cggtgaaaaa gtacgtggct ttttcgtcgc tgttgagccg
      541 ctcgtcatac atcccgatgg atttcatcat cgccagatgc tgggcccggt cgacaaaaca
      601 gaaaacatac gccccggtct gccaggcgag caccaccatg tcttccagat gctcgctggt
      661 tggcataatg cagcgctcgc cgtctttgtc ttccacgtcc tcaataaata ccatgtacag
      721 cgccataatc cctcactccg ttgtcataaa tttaaagcac gcgaacagcc tctcccggtc
      781 cagctccacc gttttgcggg cctcgccggt ctgtggattc acgatttcat ccaccgccag
      841 aatcgcccgc tgtacccgga caagcgtcgg ccggtcaggg cagatgtacc agatttcatc
      901 gatgccgttc gcccggcggt tgaacagata cccggacacc acttcctgat accgcctgcg
      961 gctcttgacg gtgcgctcta cctcaaatgc caccgttctg ccgtccagct ccgccagcgc
     1021 gtccggcact ttcatccctt tcagggccat acggtgcagg taccgccagc cggtggcccc
     1081 ccggccctcc atggagaggc gcagggactg cacggccagc cggtgcggca gctgcgccgc
     1141 cgacaccctt gacggctgaa acggaataac gtccgtcagc ggctcgtcct cgtcgaacga
     1201 gaacgccacc ccgtgcggcg tcagccccca cacgctgacc tgacggccgt caaccgggta
     1261 tttcgcccgg accagcagcc ctttcgccac cagactgtcc aggaagcggt aaaccggctg
     1321 taccgccgca atccccagcc gtcgcgcaac cagctccgcc gtggaacaca gctcttcccg
     1381 cagccagcgc agggtcagcc gtgttttctc cgcgttccgc gcctgccgct tcgcgtggct
     1441 gacgttgtac tgctccagtg tcatcagtaa agccccgcaa aatcatcatc cgcttttggt
     1501 ccgccgccgt cggccggagg caggtactcc gccagcgggt ccgttttcgc cagcaccggg
     1561 aaggttttca gcgaaatttc ccggcgctca acccgcagcg ggctgctgaa cgccagccgc
     1621 gccacgccca gcccggtcat caccgcgaac ccgtcaggca ggtgttgcag ggtgttcacg
     1681 tcaaacagcg gccgcgcctc cttctggatg aagctgtcgc cgctgatgtg ctccacgttc
     1741 cccgcctccc ggctggcccg gcggcgctcc acgtccaccc ggatctcccc ggtctggccc
     1801 gccgcccact gcgcggactc ctggctggcc gcgcgataga accagcggat cggcgtgtta
     1861 tccagtaccg tggttttcac aaaatcagcg ggcagatcct gcccgcactg accgaagtcg
     1921 cccagcgact ggtgcgcgag cagcaggttg cagtcccggc tgcgcagcgt ccccagcgcg
     1981 ttcagcacgt acttcgacag cagatattta atctcatcga gcatgatgct cgcgtgcggc
     2041 cacctccgga actcatcccg cgcaatgatg atttgcgcgc agcgggcaaa cagcatcttc
     2101 tgcacccgga tcaccgcctc atcgtccatc gagccgatga cgtacaggca gccgccgccg
     2161 ttgattatcc ccgccacatc gataccgctg tccgtctgca ggacgctcag gtcggccacg
     2221 ttctccagct tggtgatcag ccccttcacg tcctttttca gggcctccgg caacgcgtac
     2281 gccgccgcca gcacctgctg aatgtttgcc ccctgcggga actgctccgc aatcagcttc
     2341 gccgctttct gctcgctgat gcggtagtgg tccgccacgt cgccggtctc actcaggtta
     2401 aacccggcaa ccagcagatt cttcagggcg tactggtcga gatcgcggaa cagattcagc
     2461 tgcgccggtt tccccggccg cagatccagc agggtgaagt ccgggcagtg cgcccggaag
     2521 acgtgcggcg cccacgcgtc ccctttgggg tcgaagtaca ccacagcgtc cccgaaatcg
     2581 cgtacgcagc gaatgagcgc gttggtggcc atcgcgctct tgccgctccc gggcaggccg
     2641 aggatctgaa tattggtctt gcgccaggta tcacgaggga ccagcaccgg ctggccctgc
     2701 tcatccttac ccagaaacac cgcctgctgt ttccgggcct gctccgtgga aaaatcggcc
     2761 agcgcgttaa ccggtaccgg ctccccgaaa tgcaggtggc ggacgtctgt catttctccg
     2821 gtccgctggc tgctgcgccg gctctcctga ttcagtttcg cgttcaccgc ctcgtcagcg
     2881 gccgcaaaat gaccatcaag cagcaggaac gccagcagcc gcgccagaag gacgccggcg
     2941 acaagccctc cggcacacca ggctatcccg aacaggttag ccggctgcag caggtaataa
     3001 tctgcgttcg catagaggtc cgggcgcagg acggtttctc cccagctctc gataaacacg
     3061 gcgacaacca gcccccccag aagggccaga ccggcagcgc cggtcagcca gtacgcgtag
     3121 ttccagacat gtcgccgtgc cctgaccatc cgcccgggtg aatccagcga ggccagcacc
     3181 gaaaatggca cagtccggtt attatcgcgc ttatagcgat cggcatactc ccgccgcgcc
     3241 ttatggctca gccccacaaa acgcattcgg ccggcccaga gttcagccag accgaaaaac
     3301 aggccgataa ccagaaaaaa accggccaga ctgtaatact cccggtacag ccagctgacc
     3361 cacgggtaaa tctgccgggc cagctccggg atatgccact gccaggcaag cgaatccgta
     3421 ttaaacatgc aatctctctc cttcagacga cgggtttacc ggcgtgatgc gcagcccccg
     3481 ctgcacaagc cgcggacatg ccccaaggca tggcggcggc ggtcacgtcc gtggtgattg
     3541 cgttcagctt tcaccgcgga ggccagcctc tttccgcccc atgcggaaaa ggcgcagccc
     3601 cggaggaaca acgagcacgc cccagcgtgt gagaggaaaa ggcggcaccc cagtgcggtc
     3661 ttttccggtg ttccggcccc cgtcagagtt cgccatagcg agcggctgac cacccgcaaa
     3721 atcaggagca gctttgctgc ttcgtcaggt gcgtccagca ccgtggattt ttgtgggtgg
     3781 tcaggcgtga gcctgacggg ggccgggtga atttctggcg tggccggcgt ccagccggac
     3841 agagccagaa agaggggaaa agaccgtgcg tcggtggcgt ccagccagcg taagcaaggt
     3901 cttttcccct gaagtcagcc acgccccagc ggggatgact tgcatttctc cgcaaggaga
     3961 aaaaagcaac agagataggt taacgtaaaa tgttcagcga aaaaccgaca tggttcacct
     4021 atcctgataa ttgatcgtca gggaatagaa agacttaatc aggggacaat atcccacatc
     4081 agcgcgtccg gtgaccgtct tgcaggtgcc ggggaaattg cgcctcctcg ctcactcggt
     4141 cgctacgctc cgggcgtgag actgcggcgg gcgctgcgga cacatacaaa gttacccaca
     4201 gattccgtgg ataagcaggg gactaacatg tgaggcaaaa cggcagggcc gcgccggtgg
     4261 cgtttttcca taggctccgc cctcctgcca gagttcacat aaacagacgc tttttcggtg
     4321 catctgtggg agccgtgagg ctcaaccatg aatctgacag tacgggcgaa acccgacagg
     4381 acttaaagat ccccaccgtt tccggtaggt cgctccctct tgcgctctcc tgttccgacc
     4441 ctgccgttta ccggatacct gtccgccgtt ctcccttgcg ggaagtgtgg cgctttctca
     4501 tagctcacac actggtatct cggctcggtg taggtcgttc gctccaagct gggctgtaag
     4561 caagaactcc ccgttcagcc cgactgctgc gccttatccg gtaactgttc acttgagtcc
     4621 aacccgggaa agcacgcaaa acgccactgg cagcagccat tggtaactgg gagttcgcag
     4681 aggatttgtt tagctaaaca cgcggttgct cttgaagtgt gcgccaaagt ccggctacac
     4741 tggaaggaca gatttggttg ctgtgctctg cgaaagccag ttaccacggt taagcagttc
     4801 cccaactgac ttaaccttcg atcaaaccac ctccccaggt ggttttttcg tttacagggc
     4861 aaaagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctactgaac
     4921 cgcgatcccc gtcagttcag aggaggacga tggtgccacc gttccgccct gaacatcccg
     4981 gatataattt gcctgacatg cggccagaag aaagccagca acaatcaata taaaaagaaa
     5041 aatagtcttt acctttttca tatactttcc ctgtatttaa agactccggc attcatccct
     5101 gaacaccgga agtcagtatt tcaaatcatc cttgataatt ttttgttacc agttaccgtc
     5161 ccgataatca aaagctatat aacaattatc cacatcaggt tttatttgat ttttaaaaaa
     5221 tggctgtagt aaaggaaccc attctttctt caaatcaaac tcaccattat taatattatc
     5281 ctttaaaggc attcccaagc tttcaatgac agaaccatcg tcaccaaagt ctttagaata
     5341 ctcttcccct ttaaactctt cagttttctt atcaaaccat gtcaaacgta atttaagccc
     5401 catatttacc tctcaaagat attttttaat gttccgtttt ggatctggcc ctttaacctg
     5461 cttacccgtt ttgggatcaa atgcgcctag gtgttcgcca tcactggcac gatacccttc
     5521 aagctcaccg tgctgggaat cccattcata aattttacgt tttttatcgc cataccatcg
     5581 ggcccgctta ccaccaccac cttgtttagg tgttttagga tcacctcgct ttaactctcc
     5641 aagtcccttt atgtcttctg ttttaggtac tggatgataa tcatggccgt agtctttagt
     5701 ccccttgcga ggcttgttct tttcctcatt taacttattt tctgcatcct tcgctttttt
     5761 ctccttttgt ttccggctct ccatcgcccc ggtaagtgca gccttatctc tggataagga
     5821 ttcattcatt gcgtttaaag atgcctgagc cgcttttaat ttctcattct gattctgaat
     5881 atccttatca agatagcgaa cctgatcctg ccacccggag ccatattcat gaggcgggtt
     5941 ataagtcaca aaatcatctt tatttgcctc tgcctctttt acctttttat cggcttcctg
     6001 aacagcagcc cttgcaccgg ggagcgcctt ctctgtcgat gcaatgcggc tgtttaaggt
     6061 cgcaatattt ttatcttcgg catccagctc agctttcgct ttatcatagt ctcttttcag
     6121 cccctcttcc ggatgagcgg cgtcccatgc ctgctggcga cgcttttctt cctcctgacg
     6181 ctgttttacc tgtgccgggg taagaacatc tgtcaccgac acataaaccg gcttctgtcc
     6241 ggtctctttc gggaaacgaa taacggcctc acgggagtta ccaccggctg taaaaccagc
     6301 cggacgtgaa tcaccttttt cagcaacaat gccttttggc ggggctttcg ctgtcggaac
     6361 acctttcggt acgctcacct gcagagacgg gagacccgga atcgacacac tgaatacccc
     6421 cggacgtttt gtcggtttcg catccaccac agggacactc attggccgac ctgcgacaac
     6481 cgcaatatgc tgccgctcat ccttcaccac atccacaacc cgttgtctga cacgaaccgt
     6541 cgcctggtcc agtggtaaag tacttgccgg agtctccgtc accgcatcgg ccggtaatga
     6601 cgtcataatt tttgacatca ttttcggatc atcttttgca atctcagaag gcagcacacc
     6661 gtagatcgca atcccccaca gaccaaactt aaacggcccc ttcagggcag ccagcacatc
     6721 agcaacggcg gccgacaacg catcgccgga aactgataaa gccagtcctt cagcccccgg
     6781 agtagccagg gcaggcagac cgaaggccat cgcagtggcc gaagactgtc cgccaccaga
     6841 gctaccgcta tgattgccgc tgttaccacc attaccaccg ggtttactcc cgccgttacc
     6901 aaactccccc ggattatagt tatgaatatc tccattcggc gtatgcgtcg taccccagcc
     6961 cggcccgctg tttggatccg taccaccacc tgatgaaggg ccacctttac cagacgtacc
     7021 gttcacattc ccactggcct gaccaccatt atgtcccaga cctgaattac ccggacctcg
     7081 accgtctcca ccactcatac cgttttcctc tttgacattt aaaacaataa attaaagcca
     7141 acatgtcgcg gtagggatac ccgttaccgg ataccccccg cacagatccc ggcgtgcgcg
     7201 atttacgcac cgggctcctg cctcgggtgt ctggcggtga accgctccac aggccatgga
     7261 tgaagaaccc gaacccttgg tagccatgcg gctgccagtt tgtttgcttt cgtccaggtc
     7321 gtatcatcct tctggctcct gcgcctgagc gcccggcgcc agaggtttgt tacgtgtgtc
     7381 ctgaacttct gcatggtggg gaagttgccc ggtaccgagt gatagttcag gtatccctga
     7441 accactctcc tgagccattt tccctgttcg gggattgagt aatgccagcg ccttcgcaga
     7501 ccgtctttga tggctttcag agttgccgtc atccgatccc ggcgggtctt tcgtatcagc
     7561 atgaacctgc cgttgcgatc tttcccgctg atgtgcgtga acccgaggaa gttgaacgtt
     7621 tctggtttgc cttttcccct gatggcacgg ttttcggcag cgaagcggcc gaactccatc
     7681 agacgggttt tctccgggtg aaccgtgagt ccgaactccc tcagtctgcg ctgcatggct
     7741 atacggaagc gccgggcatc gtatcgtttg tcgaacccga tgacgatgtc atcggcgtat
     7801 ctgaccatta ccacattgcc tgtggcatag cgacgtcgcc actgatgcgc ccacagatcg
     7861 aagacgtagt ggaggtatat gtttgccagc agcggtgaga tgaccgcacc ctgtggggtg
     7921 ccttcctccg ttgctcgcca ttgaccctcc tccgacgtcc cggctgtgag ccacttacgt
     7981 atgagcctga ttaccctccg gtcgccgatc cgatgctctg tgaacctgat cagccattcg
     8041 tggctcaccc tgtcgaagaa ctgactgatg tcggcatcca gtacccagtt tacgttagtg
     8101 cgtaccagcc ctgtggccag tgcgtccagt gcatcgtgct ggcttcgccc gggtctgaac
     8161 ccgtatgaga accccataaa gtcgttttca tagactgcgt tcaggatttt caccagcgca
     8221 tactggacga tcttgtcctc cagcgaggcg atgccgagcg ggcgttgttt tccatccgct
     8281 tttgggatgt agtgacgcct gccgggctgc gccctgtagc tgccctgatg tagcctccgg
     8341 tgcagatctg ttatgttgtt cttcatgttt ccggcgtagt ccatccacct gatgccatcc
     8401 actccggcgg ccgctttcct gctcagggag aggaatgcgg cttccagtgc ttcgactgtc
     8461 agcaggtgga acaatgctgt aaaccgttct ttcttccgct gcttcgcagc ttcccgcacg
     8521 cgtgacagcc tctgtgacat gctttcccgg ctctgtgtcc ggcgcatgtg tggctgttcc
     8581 gcgttcccct tggccccgct ccttcgctcc actgactccg ctcctttcgg gttgttcgcc
     8641 tgcttcgccg ctactatgag cgagtccgac ttctcctctc cgtacatcac cggctatgac
     8701 tcctcgtctt cccggtgcgg gccatctccg acactggcag atggtcagag gggagatctc
     8761 ccggttcccg cgtagagatc gtattgacat gccagggtct cagaccccgc cgggtccatg
     8821 tggcactcgc agtgtcgcac cctatgatgt tgccttccgt taacagtaca acgtcggcac
     8881 ccggtaattt aatatacatt tcgtggctca atggctggcc tgtcaacacc cctgtcaacg
     8941 cttcgcccca tacctcgcgg tatgcaacgc atgactcggg gaccttgtgg attgctggtc
     9001 cttcaatggt cggggacttt caccccttga tctctaaccg gtctcccggc gcacactgta
     9061 catatacaca gtataattat taccttactc ctcccgtttt caggtgtcaa tatatgagat
     9121 tagttctcat cttcaggcac aaaaaagcgc ccggagccac ctgtaagcag atcgcgtctt
     9181 ttcccgaaaa tttctcatgg caacaaattt tattatttaa gacattcatc acacttacat
     9241 ttcagatggt atagacacat ctgaaagcta actggcatga ggagaggacc tgcggttaaa
     9301 ctgttcggga aacctgagac gcccggaggc cactatgacg cacctgaaac tgactcccgc
     9361 tgaagaaaag cgcctggcgg acgcctttgt cgctcaggca ctggccatgg gcctgaaagg
     9421 gaacaaaacg ctgccaaagg cggtaaaaca acaggtgcgg gaaagggtgc ttcgtgccat
     9481 tcaggacgaa cgtgaagcca ggcgggcgca ggaaatgcgt cgggagaaat acgaggctac
     9541 ggaaaccacc ttcacctggc agccacagcg ccgccggtaa ggggtattta ccataacgtc
     9601 gcattgcccg caccgctcag acggtctgag ggcgtttccg gcggctacgg gataaaacag
     9661 gcaacaaacc acccggaaaa ccgccggaaa cgcgcccggt aaattgtaac ccgctgcatg
     9721 cctatgcacg caactgcatg gcgggccgtt tggtgtaaaa acgatacgat aagccgtgtt
     9781 tatgtaaatg aacgtggccg taaagagtga caaatataca tgtaacattt tttactgaca
     9841 ggtcatcaac ccgatgcgtt taataccctt tatcctgctg gcattgtgca gccccttact
     9901 cctgacggcc tgtaagcatt ccaccaccca gccgccgaaa atttatactc tggataagtc
     9961 atctaaaatc agtagctacg ctgcggccat caagaccgct gttgaaatgc agatgtttga
    10021 accagaggcc tatgcgggaa aaatgtgcac cgtgcggata tccatacaac gtgatggcac
    10081 tctgaacagc gccattgctg aaggaggaga tccgaaactc tgtaaagcag caatatcagc
    10141 cgttacccac gccaaaattc cgccggcacc ggatgaaaat acctggcaaa tattcaaaaa
    10201 cgcccctctg gatttcagac catgaaagct tttactctga cagtgctggc ggccatgttc
    10261 ctcactccgg tacatgcgat accactaaaa cccgtaacca cactgcagcc aacctacccg
    10321 gtacaggccg cagcaaaacg catcgaaggt caggtgaccg ccacgtttga tattctggac
    10381 gatggacgag tggacaacgt gtcgcttacc ggctgtcacc ttttttttcg ggaaacgctg
    10441 acggcaatga ggcactggct atatcccgca gggcacccgg caatgcgaca ggttataaat
    10501 tttgaattca gtctgaaaaa tgacggctta caggatccgc gaccagtcac cacccaaaag
    10561 ctcaatgatg atggttgccg ccagtaacag gtcctttaac ccgcaggctg caatagtcct
    10621 ggatgatgag tgagcctgta gcagccctga ttagtggaag cgaagctgaa aaatgggcag
    10681 caaaagccgt atttctggca aatagcgccg ccacggatta aagtagtcat gtgtaataca
    10741 tatgactaca aaggggcttt tatgtcatca gcaacatcca tcaggctcga tgaagaactt
    10801 aaggaccggc tgaaaacgct ggccgatgac cggcaccgct cagcgcacgc gctaatgctg
    10861 gaggcaatca ccgaatacat cgaccgggaa gagaagcgca gccagtacct gcgggacgga
    10921 caggccgcat ggcagcacta tcaggaaacg ggtctgcacc ttactgcgga agaagcggaa
    10981 gcctggataa gtacctgggg aactgagaac gagcaggatg ctccgccatg ccacaggtaa
    11041 cgatttccgc gctggcacag cgcgatttac aacgccttca ggactttctc aaaaccaaaa
    11101 atcggctggc agccagaaaa ggaggtgagg tgatcgtccg ggctatccag caactgaaaa
    11161 cattgccaga cattggccgc ccggtgccgt ttctgccgct ggaatataag gaactggtga
    11221 tcgggtttgg cgacagtggc tatgtgatgc tctaccgcca cgaccgggaa atggaccaga
    11281 ttgtgatcgt caccgtcagg catcagaaag aatccgggta tccgggggca gacagcctct
    11341 aataaccgat gacgggggcg caattcattg cgtggccgtc gccggtttga caagcgcagc
    11401 gcgtcagaaa accacatcct gaacggaaaa acgggtaaaa ttcagcgata catttaccgt
    11461 gagaaattac acacctttca cacccgataa aatcaggggt atacagtggc gaaaacattg
    11521 ctcaaccttt cacaggcagc acaagccgcg ggcattacca gaagaacgct atacaatcat
    11581 gtaaaacagg gaaaggttac cgtgtcacgt gacgggaaaa acaaccctgt cgtggatgtg
    11641 tcagaactga tccgtgtata cggaaatgtg aatatccctg agaaacaaat tcccggcatt
    11701 tcacaccgtg aaaatacaca aaaaaatttc ccacaggaac agcttctggc aatgcaaaag
    11761 gaactggccg atctccggca ggctgtcacc ctgatgctgg aagataaaac atcccgggaa
    11821 gaagaacgcc gccagcacga tgatgagcgc cggaaactcc aggccgaagt ggacagattg
    11881 acaacagaac tgacacagaa gaaaaaacgc ttctggtccg gctggtttag ctgagaactt
    11941 accgaaggga atggagaaca tgacgaagca
//
