LOCUS       NZ_CP041266             6173 bp    DNA     linear   CON 20-APR-2024
DEFINITION  Enterococcus faecium strain VVEswe-R plasmid pVVEswe-R6.
ACCESSION   NZ_CP041266
VERSION     NZ_CP041266.2
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12138936
            Assembly: GCF_007917035.2
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Wagner,T.M., Janice,J., Sivertsen,A., Sjogren,I., Sundsfjord,A. and
            Hegstad,K.
  TITLE     Alternative vanHAX promoters and increased vanA-plasmid copy number
            resurrect silenced glycopeptide resistance in Enterococcus faecium
  JOURNAL   J Antimicrob Chemother 76 (4), 876-882 (2021)
   PUBMED   33367710
REFERENCE   2  (bases 1 to 6173)
  AUTHORS   Wagner,T.M., Janice,J., Sivertsen,A., Sjogren,I., Sundsfjord,A. and
            Hegstad,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2020) Department of Microbiology and Infection
            Control, Norwegian National Advisory Unit on Detection of
            Antimicrobial Resistance, Sykehusveien 38, Tromso 9038, Norway
  REMARK    Sequence update by submitter
REFERENCE   3  (bases 1 to 6173)
  AUTHORS   Wagner,T.M., Janice,J., Sivertsen,A., Sjogren,I., Sundsfjord,A. and
            Hegstad,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2019) Department of Microbiology and Infection
            Control, Norwegian National Advisory Unit on Detection of
            Antimicrobial Resistance, Sykehusveien 38, Tromso 9038, Norway
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JAN-2019
            Assembly Method                   :: Unicycler v. v0.4.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 305.0x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_007917035.2-RS_2024_04_20
            Annotation Date                   :: 04/20/2024 02:37:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,257
            CDSs (total)                      :: 3,164
            Genes (coding)                    :: 3,041
            CDSs (with protein)               :: 3,041
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 58 of 123
            Pseudo Genes (incomplete)         :: 63 of 123
            Pseudo Genes (internal stop)      :: 24 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP041266.2.
            On Jun 19, 2020 this sequence version replaced NZ_CP041266.1.
            Bacteria and source DNA available from KRES.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="VVEswe-R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pVVEswe-R6"
                     /geo_loc_name="Sweden:Halmstad"
                     /lat_lon="56.6744 N 12.8578 E"
                     /collection_date="2014"
                     /collected_by="KRES"
     gene            2..946
                     /locus_tag="FKW92_RS15840"
                     /old_locus_tag="FKW92_16695"
     CDS             2..946
                     /locus_tag="FKW92_RS15840"
                     /old_locus_tag="FKW92_16695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002303559.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRF
                     TFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKINGD
                     VENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFAYFS
                     KEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGYQFTF
                     KPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQIKQ
                     LEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
                     /name="replication initiation protein"
     gene            967..1500
                     /locus_tag="FKW92_RS15845"
                     /old_locus_tag="FKW92_16700"
     CDS             967..1500
                     /locus_tag="FKW92_RS15845"
                     /old_locus_tag="FKW92_16700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002363576.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLREQL
                     ALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATETMD
                     EQTKNWKKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            1515..1685
                     /locus_tag="FKW92_RS15850"
                     /old_locus_tag="FKW92_17175"
     CDS             1515..1685
                     /locus_tag="FKW92_RS15850"
                     /old_locus_tag="FKW92_17175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032491833.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNSQ
                     NEEVFVEKLDK"
                     /name="hypothetical protein"
     gene            2140..2364
                     /locus_tag="FKW92_RS15855"
                     /old_locus_tag="FKW92_16705"
     CDS             2140..2364
                     /locus_tag="FKW92_RS15855"
                     /old_locus_tag="FKW92_16705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="WP_002363584.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEKC
                     WVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
                     /name="class II bacteriocin"
     gene            2384..2671
                     /locus_tag="FKW92_RS15860"
                     /old_locus_tag="FKW92_16710"
     CDS             2384..2671
                     /locus_tag="FKW92_RS15860"
                     /old_locus_tag="FKW92_16710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_002363589.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKSV
                     GEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
                     /name="bacteriocin immunity protein"
     gene            3121..3501
                     /locus_tag="FKW92_RS15865"
                     /old_locus_tag="FKW92_16720"
     CDS             3121..3501
                     /locus_tag="FKW92_RS15865"
                     /old_locus_tag="FKW92_16720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_010782660.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPGDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3483..4397
                     /locus_tag="FKW92_RS15870"
                     /old_locus_tag="FKW92_16725"
     CDS             3483..4397
                     /locus_tag="FKW92_RS15870"
                     /old_locus_tag="FKW92_16725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_010782659.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKKKVRS
                     NKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTTEQPEI
                     RPTRRNHDQHVERGADYQQELSF"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4642..5331
                     /locus_tag="FKW92_RS15875"
                     /old_locus_tag="FKW92_16735"
     CDS             4642..5331
                     /locus_tag="FKW92_RS15875"
                     /old_locus_tag="FKW92_16735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002369882.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLPG
                     VIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQTEEA
                     CYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     BGC             1..6173
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          3..946
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3484..4394
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.421"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aatggctaat gagattgtaa aataccacca tgaattaaat acaattcctt taagaaaatt
       61 tacaccggtt gaaatgaatt tgtttttttc catcgtgtct agaatgcgtg aaaaagggga
      121 taagacagtt cgttttacgt ttgaccaact aaaagattta agtaactaca aagcaactgc
      181 aaatgtacga tttgtagaag atttaaaagc aacttatgaa aaaatcttat ctttacgctt
      241 tggtcgtaga agtgagtctg ggctttctta cgatatgttt gtaatgttta cagaatttaa
      301 aatcaacggg gacgtagaaa atccttacgt agatatacga atttacgaaa aagcactgcc
      361 tttgttaaat aatttagacg agtgggtacg ctattcttta caacaattca acgagctaca
      421 aagtggctat gcaaagacta tgtttcgtct gttaaagcaa tacagaacca aaggtttcgc
      481 atatttctcc aaagaagact ttcttgaatt attagatatt cctaaaagtt ataagcaacc
      541 tgatatagat aaacgagtta taaaacctat acgtcaagaa ctaactgcaa tcttcaaagg
      601 actaacaatc aaaaagaaat acggaaaagg acggggaaaa cctgttattg gctatcaatt
      661 tacgttcaaa cctgagatca aaaaagcaga cgactttaac aaacatgcac caaccaaaga
      721 agaccaaaat aaagcattgg ctttagctga taaacaaaaa gaatacgaag aacccgctac
      781 aacaattaac gaaaaaatca aacagaagaa acgagaactt gaactcctcc aacaaatcaa
      841 acaactagaa aaagagaaaa aagctcttga aaaacaaaca gagtccgcac tttcggaaaa
      901 agagaaaaaa agtttattat ctgatttaga taatttattc cagtagcaac ctaggaggca
      961 accagtatgc aaccacaaga aacctttaca ataaaggaat tgtcagatct ttttaagatg
     1021 agtaggcaag caatatcgaa acatatccaa aaattagatt cttccatgat agctaaaaat
     1081 gagcgtggtt ataaggttgt attgcgttct ggagtattgc aacttgctcg caacttagac
     1141 aatcaaaggt tgctagataa gttgcataaa catgaacaaa acaacgataa aaatgcttat
     1201 ataccaaaca aaaacgtggt tgcaaacaag ttgctagatc agttgcgtga agaaaatgaa
     1261 tttttacgag aacaacttgc cctcaaagag caaaataatt ctcatataca aaaactactc
     1321 gaccagcaac aacaactcac cctgcaagcc aaccaacaaa tcgaaaaact tcaggagcaa
     1381 ctgcagttga cttatacgga agagtcttca gagaatagtt caacgactct ttctgaagca
     1441 acagaaacaa tggacgaaca aacaaagaat tggaaaaaat ggtggcagct atggaaatga
     1501 agaaggaaat acttatgaca aaaaaacact tgctattcgg ttctatccta ctaggaatat
     1561 taagctttag cccaatgtta ttatcagtgg ccaacaaagg aaataaaaaa ggggaactaa
     1621 aactcaatcc taattactac aacagtcaaa atgaggaagt atttgtagaa aaacttgata
     1681 aataagaaaa gactgctcgt acgagcagtc tttctgaaag agattatcct attattaacc
     1741 ttagaaaaga ataataaatg aatatgagat atgtaaaaag cataacaaac acaaacaaaa
     1801 ataacaagta tttaaaaaat gaccgatcaa tattcacaaa gaattagaaa gaaacaaccg
     1861 cagaaaacca agccttaaaa agagctttta acctagacca ataaaaattc acaaaatcag
     1921 cgcctggatc ccttggtaca ggcgttactt ggtagtatac acattatgtc tactagctgt
     1981 ttaaataact gatcttataa ttttatttaa acgattatga attcaaaact actttttatg
     2041 acgttaatca ttgttatata ataaaatgtt atataataaa attactaaga ttatttaata
     2101 taacaagcaa ttttaaaaaa acgaaaagga aaaacaatca tgaaaaagaa agtattaaaa
     2161 cattgtgtta ttctaggaat attaggaact tgtctagctg gcatcggtac aggaataaaa
     2221 gttgatgcag ctacttacta tggaaatggt ctttattgta acaaagaaaa atgttgggta
     2281 gattggaatc aagctaaagg agaaattgga aaaattattg ttaatggttg ggttaatcat
     2341 ggtccatggg cacctagaag gtagtggtag ggaggttatt ataatggatt ttactaaaga
     2401 agaaaaactt ttaaatgcaa ttagtaaagt atacaatgaa gcaactatag atgactatcc
     2461 tgacttaaaa gaaaagctct ttctttattc taaagaaatc agtgagggaa aaagtgttgg
     2521 tgaagttagt atgaaattaa gtagttttct tggaagatat attttaaaac ataaatttgg
     2581 attacctaaa tctttaatag aattacaaga aattgttagt aaggaatctc aagtatatag
     2641 aggatgggct tctattggta tttggagtta attctttgga ataaaatgaa aatgggactg
     2701 aagaatcaat tctgattcat cagtcccata aattatagag ccgaaaaatt atcatattta
     2761 tagatttatt aatagtaaat ggcataagaa tctttgaaca acgaaaaaaa tgtagctcta
     2821 cctaattatt gatttgcaac aatttcaccg aaggtgaaac aagcgaaact ttacaaaaag
     2881 tggctagcat ttgctagtct tttttgtatt gtggagagtg ggggggaggg ggattcaagg
     2941 gggagcttgc gcccctttga caagcggtta tttgcaccaa atacgtaggt atttggctag
     3001 caaatataga gcttgccaag ccctttagtg agctttagtg agctttactt attgtacctt
     3061 tttaggtaca atggtattgc gaacgatacg tccgtaggac gtactgaaag ggtgggactt
     3121 atgagcgaac aaaatcaata tttggctagc aatcattcta aaaaatatac ctatcgatcc
     3181 gagccaaaac aaatcagttt tcgagtgagc gaatccgaat ttgcaaagct gaagcagtca
     3241 gctgaagctt tgcaaatgag tgtgccggct ttcgtgaaag ccaaggcaca aaacgcgcgt
     3301 ctggtgacac cgaaagtggc gccagacatt gctcaggcaa tcgcgcgcga tttagccaaa
     3361 gctggcggga atatcaatca aattgccaag tggtgcaaca cacaccaaca cgacgttgat
     3421 cctggcgacg cccaacgctt atccgaaaac ctaaaaatca tgcaaaagga gctacaaaag
     3481 atatggcaac agttaaagta agccgaacca cttcttgtag ccgagcgatc aattacgccg
     3541 aaccacgagc aaccgtcaaa acagggataa actgtgacat tgattatgcc aaaagcgaaa
     3601 tgaaacaaat tcgcatgctg tatggaaaag acgatcgtgt acaagcacac ttattgatcc
     3661 agtcttttcg accaggagaa attaccgctg aaaaagccaa tcaactaggg gcagagtatg
     3721 cgaaaaaaat agcccctgac caccaaatcg cgatttacac acatacagac aaagaccata
     3781 ttcataacca cattgtcatt aactccgtca atctggaaac tgggaacaaa tttcaagccc
     3841 atggtcaagc atttttaaac aaatgttatg acctaaatga cgagatttgt cttgaacatg
     3901 acttaacaat cactgaacga gggaagaaac ccgaaaaacg aaccatgtct gaaatcaaat
     3961 taaaagaaaa aaatgaacct gtttggaaag acgaaattcg gtttgcgatt gatcaaacaa
     4021 tgaaaaacaa aaaaactcga acgtataacc aattttgtga ttctcttaaa acattcggca
     4081 ttcattgctt gaatcgtggg aaaaatttta cttacgaatt gatccacaaa aagaaaaaag
     4141 tacgatccaa caaactagga aaggattacg aaaaggagac gattttacat gagctggaca
     4201 gacgagaacg agcctacaat caacaacgaa ccaacgagct acgaagagct cgagctagca
     4261 ttcgcgaaac tgcaaaagaa gtacgaagag aacaacgaac tcttacgact gaacaaccgg
     4321 aaattagacc gactcgaaga aaccatgacc aacacgttga aagaggagca gactaccaac
     4381 aagaattatc gttctaaaac gttacaagac atagaaaatg aaataaagaa acaacgtgct
     4441 tttcttgcac cttataccaa agaaagaata caaactacaa ttgattcaca agtcaaaaaa
     4501 ttccaccatg cgctattaaa ccagcgaaat aaagaacaga aaaaagtaat tatgcagcga
     4561 tcaatggata ttcttgcccc cttatgttgc ttaatcatga cactgttagc cttttggctg
     4621 gtatggggag aataaataga aatggcattc tatttaccct atctactcat ttttgccagt
     4681 attttaggaa ttatttggct tagctataag gcttatcaaa caagatattc attaaaaggc
     4741 tctaaaattc gatttaagag gtttttattg ccaggggtta tatttacatt atcaatcgtt
     4801 ttaagtggtt tattcggcgt tttagaggga aataaacgta tttcaagaag tattttatta
     4861 ggggatatca ctcaaaagta tgaagcagca tgtgataaaa agaaaaagga gcaagtgtta
     4921 acacaaaaaa tggaagagtt taccacttgt tatgaggaaa tgaacgatat ttttgtgaat
     4981 caagaaaaac gtttgacgga taaaaacatg gaaaaactca ctcgtcttta tcaaaacttg
     5041 ccagaaaaac agcaaaaaga ggttcaagac aactatgagc gaaccaaaaa agacgtgcaa
     5101 tattttaaag atacgcagac agaagaagct tgttataatt tattcagtga tacaatccct
     5161 ttttcaacta gtgaacagga acgaaaagaa cggcaacaat cagttaccta cgaacgttat
     5221 aaagcactgc ttcagcaagc aacgaacata caaaatccaa cgaaaaaaga aacggcatta
     5281 aattatctaa aaagtgtcaa ggaatggtta gatcagcaac agcaaaattg aatttgatcg
     5341 gcaatcatgt taaactaaac atgcaaatga tcaaacatta gatacacgaa agccccagag
     5401 aggtcggatt ctctggggct ttttttcagg ttgatgtcgc ctttattgat tttacttctt
     5461 gtttcggtta ttcaaccaat aactgaataa agagacacaa acttctataa gaagggggca
     5521 aatgaacaat tcaaacatta gatacacctc cttccagtcg caagactgtc ggataggcga
     5581 caagttatta tatcttaaaa aattaaactt gtgtggtgtt tttaagtatc gcttatttta
     5641 gggttgttag ggcttgccct gaccgcctgt aaggcgcttt aaagctagat aaggattttt
     5701 atccaagact gtcttaaaac ggcttagata agcattcaga accgctcaaa ggcctcttta
     5761 ccaacatgtt tttagcaaag gagcctttag gcgactgcgc agtacaatgt gagagcgtag
     5821 cgatcacacc tttttggcga ccgtaggaag caaggttttg gattggcagt tttgaaaaat
     5881 gcgcgaacct gtttaaagaa aaaagaagtg gtttttaggc gggaaaatta gcccccctct
     5941 ttttatatct ctttttaaac ctctttttaa acctctttac cgtaagctgt gtccttactc
     6001 taccaatgga tacagaatgt tatgaaggac attttgtctg tttatgaagg acattttgtc
     6061 tgtttatgaa ggacattttg tctgtttatg aaggacattt tgtctgtttg aacgtcaaat
     6121 atgaatgtct tgacatttaa aaaatgtcat tcataattgg attgaggtga tta
//
