LOCUS       NZ_CP041265            14136 bp    DNA     circular CON 20-APR-2024
DEFINITION  Enterococcus faecium strain VVEswe-R plasmid pVVEswe-R5, complete
            sequence.
ACCESSION   NZ_CP041265
VERSION     NZ_CP041265.2
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12138936
            Assembly: GCF_007917035.2
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14136)
  AUTHORS   Wagner,T.M., Janice,J., Sivertsen,A., Sjogren,I., Sundsfjord,A. and
            Hegstad,K.
  TITLE     Alternative vanHAX promoters and increased vanA-plasmid copy number
            resurrect silenced glycopeptide resistance in Enterococcus faecium
  JOURNAL   J Antimicrob Chemother 76 (4), 876-882 (2021)
   PUBMED   33367710
REFERENCE   2  (bases 1 to 14136)
  AUTHORS   Wagner,T.M., Janice,J., Sivertsen,A., Sjogren,I., Sundsfjord,A. and
            Hegstad,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2020) Department of Microbiology and Infection
            Control, Norwegian National Advisory Unit on Detection of
            Antimicrobial Resistance, Sykehusveien 38, Tromso 9038, Norway
  REMARK    Sequence update by submitter
REFERENCE   3  (bases 1 to 14136)
  AUTHORS   Wagner,T.M., Janice,J., Sivertsen,A., Sjogren,I., Sundsfjord,A. and
            Hegstad,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2019) Department of Microbiology and Infection
            Control, Norwegian National Advisory Unit on Detection of
            Antimicrobial Resistance, Sykehusveien 38, Tromso 9038, Norway
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JAN-2019
            Assembly Method                   :: Unicycler v. v0.4.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 305.0x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_007917035.2-RS_2024_04_20
            Annotation Date                   :: 04/20/2024 02:37:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,257
            CDSs (total)                      :: 3,164
            Genes (coding)                    :: 3,041
            CDSs (with protein)               :: 3,041
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 58 of 123
            Pseudo Genes (incomplete)         :: 63 of 123
            Pseudo Genes (internal stop)      :: 24 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP041265.2.
            On Jun 19, 2020 this sequence version replaced NZ_CP041265.1.
            Bacteria and source DNA available from KRES.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..14136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="VVEswe-R"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pVVEswe-R5"
                     /geo_loc_name="Sweden:Halmstad"
                     /lat_lon="56.6744 N 12.8578 E"
                     /collection_date="2014"
                     /collected_by="KRES"
     gene            complement(74..482)
                     /locus_tag="FKW92_RS15765"
                     /old_locus_tag="FKW92_16595"
                     /pseudo=""
     CDS             complement(74..482)
                     /locus_tag="FKW92_RS15765"
                     /old_locus_tag="FKW92_16595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(484..764)
                     /locus_tag="FKW92_RS15770"
                     /old_locus_tag="FKW92_16600"
                     /pseudo=""
     CDS             complement(484..764)
                     /locus_tag="FKW92_RS15770"
                     /old_locus_tag="FKW92_16600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(1140..1664)
                     /locus_tag="FKW92_RS15775"
                     /old_locus_tag="FKW92_16605"
     CDS             complement(1140..1664)
                     /locus_tag="FKW92_RS15775"
                     /old_locus_tag="FKW92_16605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_060800956.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINET
                     GQKAIKSMFFNENENSNQQLVGEKTKTETHKNANQVPELEVLSLLEQQLNQKDMQLQEK
                     DEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQS
                     KTEKKWWQFWQ"
                     /name="DUF536 domain-containing protein"
     gene            complement(1681..2649)
                     /locus_tag="FKW92_RS15780"
                     /old_locus_tag="FKW92_16610"
     CDS             complement(1681..2649)
                     /locus_tag="FKW92_RS15780"
                     /old_locus_tag="FKW92_16610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002347614.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQF
                     TFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKIDGK
                     SDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFAYFS
                     KEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGYQFTF
                     KPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELLQQLEK
                     LEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
                     /name="replication initiation protein"
     gene            complement(3819..4262)
                     /locus_tag="FKW92_RS15785"
                     /old_locus_tag="FKW92_16620"
     CDS             complement(3819..4262)
                     /locus_tag="FKW92_RS15785"
                     /old_locus_tag="FKW92_16620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002318191.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGT
                     WQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLT
                     LRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
                     /name="hypothetical protein"
     gene            complement(4235..4735)
                     /locus_tag="FKW92_RS15790"
                     /old_locus_tag="FKW92_16625"
     CDS             complement(4235..4735)
                     /locus_tag="FKW92_RS15790"
                     /old_locus_tag="FKW92_16625"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_060800863.1"
                     /translation="MNYNKNLLSHIVYFNYIIIGVYHLNLTQRRGYFETQKENLSRSPL
                     FLDDWNSQIESKSLNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGY
                     GNYSNSRHALALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTI
                     TNW"
                     /name="hypothetical protein"
     gene            complement(5277..5693)
                     /locus_tag="FKW92_RS15795"
                     /old_locus_tag="FKW92_16630"
     CDS             complement(5277..5693)
                     /locus_tag="FKW92_RS15795"
                     /old_locus_tag="FKW92_16630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002305824.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSYE
                     PDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKIEN
                     QLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
                     /name="hypothetical protein"
     gene            complement(5695..5976)
                     /locus_tag="FKW92_RS15800"
                     /old_locus_tag="FKW92_16635"
     CDS             complement(5695..5976)
                     /locus_tag="FKW92_RS15800"
                     /old_locus_tag="FKW92_16635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002347613.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESDF
                     DRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
                     /name="hypothetical protein"
     gene            complement(6349..6882)
                     /locus_tag="FKW92_RS15805"
                     /old_locus_tag="FKW92_16640"
                     /pseudo=""
     CDS             complement(6349..6882)
                     /locus_tag="FKW92_RS15805"
                     /old_locus_tag="FKW92_16640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /name="DUF536 domain-containing protein"
     gene            complement(7948..8154)
                     /locus_tag="FKW92_RS16325"
     CDS             complement(7948..8154)
                     /locus_tag="FKW92_RS16325"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_033794861.1"
                     /translation="MGTAGYGNYSNSRHALALFGWVNQGGYQTYYVWNPWYNYPTVISA
                     NAGPVTIPVPGGGFTWDNTITNW"
                     /name="hypothetical protein"
     gene            complement(8995..9401)
                     /locus_tag="FKW92_RS15815"
                     /old_locus_tag="FKW92_16655"
                     /pseudo=""
     CDS             complement(8995..9401)
                     /locus_tag="FKW92_RS15815"
                     /old_locus_tag="FKW92_16655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(9403..9685)
                     /locus_tag="FKW92_RS16330"
                     /old_locus_tag="FKW92_16660"
                     /pseudo=""
     CDS             complement(9403..9685)
                     /locus_tag="FKW92_RS16330"
                     /old_locus_tag="FKW92_16660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(10058..>10255)
                     /locus_tag="FKW92_RS16335"
                     /pseudo=""
     CDS             complement(10058..>10255)
                     /locus_tag="FKW92_RS16335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /name="DUF536 domain-containing protein"
     gene            complement(10458..10580)
                     /locus_tag="FKW92_RS16400"
     CDS             complement(10458..10580)
                     /locus_tag="FKW92_RS16400"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_275552314.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKFLTKIENFGFRKSETSL"
                     /name="hypothetical protein"
     gene            complement(10597..11566)
                     /locus_tag="FKW92_RS15825"
                     /old_locus_tag="FKW92_16670"
                     /pseudo=""
     CDS             complement(10597..11566)
                     /locus_tag="FKW92_RS15825"
                     /old_locus_tag="FKW92_16670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(12737..13186)
                     /locus_tag="FKW92_RS15830"
                     /old_locus_tag="FKW92_16685"
     CDS             complement(12737..13186)
                     /locus_tag="FKW92_RS15830"
                     /old_locus_tag="FKW92_16685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_254700834.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGT
                     WQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLT
                     LRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFLKLIDE"
                     /name="hypothetical protein"
     gene            complement(13159..13365)
                     /locus_tag="FKW92_RS16340"
     CDS             complement(13159..13365)
                     /locus_tag="FKW92_RS16340"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_033794861.1"
                     /translation="MGTAGYGNYSNSRHALALFGWVNQGGYQTYYVWNPWYNYPTVISA
                     NAGPVTIPVPGGGFTWDNTITNW"
                     /name="hypothetical protein"
     Typing          complement(1686..2649)
                     /name="rep_cluster_1093"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.782"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(10602..11566)
                     /name="rep_cluster_1093"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.465"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gtctttactg gaagactgat ccctaaaaag aaatcgagga attttttagg gatcggtttc
       61 ttttttttat cttttagaaa gtttgttggg tcaaaatttc cagaagtaaa ggtttgattg
      121 gtttcttttt gacgattttt gggttgattt cgtcgggaag gattgagttg attttcaatt
      181 tttctttcac ttcttgatac actttagaat ttgtaccgat aagaacacca gtatctttta
      241 gatataaatc agcaaattct gccatgattt ctgtcatgat agaaatttct tttcccatgg
      301 cattgagttt tgcttggtta ctggttgtgg ttgtattggt atcaggttca taactcatac
      361 ataaaaacga atggcttctg aataattgtt gacgtctgga tttttttttc gatgatggaa
      421 tctaaaacgt caacgtgact gggataaagg gttacgtgtt tttgaacaga agtttttctc
      481 atgttatatt cctccgataa gtgtttcaat gagttggttg tttgtgtgaa catagtcatt
      541 cagtatgttc gtattttctg tttgaatggc aataaatttt tcgagtaata aatcgaattg
      601 atctttttgt aattcataag ttgacggtca aaatcacttt cgattagaac ttttagatat
      661 ttatttcgac tccatttttt gcccgttttg gttgtgagtt cctgacaacg tttatctatt
      721 cttgcaataa ttttgggatt gatatttcga actaaaatgt ccatggaaat tttcctttcg
      781 tatcagcgta taatttatgt ataccataca aatatagtat ttgtatagct atttttcgcc
      841 aaaacactat acatagccta gccacaatgg caaaaaagtg atagcaaatt tttttctgag
      901 aaacgtgata ggcacagcct atctagcacc tttcgtaaga aagtgatacc acgtttcctt
      961 atgctctttc aaaattcaga aaaatcagct gtaaaacgag tgcagtccga gtaagattca
     1021 acgaaaaaac ggggattggg gcgccaccaa aatatcgcaa catgggtcca tgttgctctg
     1081 ctcgcaaaag caaaaagaag aataaaacca gcaattttta ttaatttaaa ttgctggttt
     1141 tattgccaga attgccacca ttttttctcc gtctttgatt gtttttctat cgattctgtt
     1201 tcttcagaaa gagtcgttga tttattttcc gaagattctt ccgtataagt caactgtagt
     1261 tgttcctgca gtttctcgat ttgttggttg gcttgcaggg tgagttgttg ttggtggtcg
     1321 agtaattttt gcatttgttt tatttgctcg tctttttctt gtagttgcat atctttttga
     1381 tttaactgtt gttctaggag agaaaggact tctaattcgg gaacttggtt ggcgtttttg
     1441 tgggtttcgg ttttcgtttt ttcgccaact agttgttgat ttgagttttc gttttcgtta
     1501 aaaaacattg attttatagc cttttgtccg gtttcattaa tcacaaactt gtttccgatt
     1561 ttcgaaaacc aaagttttcg attttcgtca gaaacttttt tgttaattgc tgttttacta
     1621 actcctaatt cttcagcaag ttctttgatt gttttttctg tcataaaatc taaaaacccc
     1681 tcaatctttg aataagtttt gaagattgtc taataagtct ttttttagtt ctgaaggaat
     1741 atcggaattt atctctgatt tttgtgcatt attttcttta ttttgggtat tttgttctct
     1801 ttctaacttt tctaactgtt gtaaaagttc caattttcgc ttttgttctt tgattttttc
     1861 ttgtaatgaa gtaggtgctt tttcagattg aacttgttgg ttagctagtt ctaatgcttt
     1921 gttttgttct tcttttgttg gggcatgttt gttaaagtcg tctgattttt ttatctcagg
     1981 tttaaatgta aattgatagc caatgacagg ctttccccgt ccttttccgt atttcttttt
     2041 gattgttaaa cctttaaaga ttgcggttag ttcttgtcgt attggattga acacacgttg
     2101 atcaatcgta ccttggttgt aacttttagg aatatcaagt aattcatgaa agtcttcttt
     2161 tgaaaaataa gcgaaacctt tggttctgta ttgttttagt aatcgaaaca ttgtttttga
     2221 ataactgctt tctaattcat tgaattgttg gagagaatag cgcacccatt cttcaagatt
     2281 attaagtaag ggtaaggctt tttcatgaac ttgtatttca gcaaaaggaa tatcagattt
     2341 tccgtcgatt ttaaattgat taaataaaac aaaacgttca cgttgcaaac cgtcagcact
     2401 tcttctacca aaacgtaaat ccattaattt gtcatacgtt gtttctaaat catctatgaa
     2461 tcgaacattt gcagttgctt tataattgct taaatctttt aattgctcga atgtaaattg
     2521 tactttttta tcacctacat cacgcattct tgatacgata gaaaagaaca aattcatttc
     2581 gactggtgta aattttcgca acggaattgt atttaattca tgatgatact ttacgatttc
     2641 attagacata tttaaaatag ctcctttctt actcgcacaa gagatacacg ttatgaaata
     2701 caaaatgcct gtttatctac atcttatatt aacatcgtcg atgtactaat tcaatgtaca
     2761 aacagacaaa acgatgtaga taaacagaca aaacgatgta gataaacaga caaaacgatg
     2821 tagataaaca gacaaaacga tgtagataaa agcgtcattc ccttgggaga gtaaggatat
     2881 agccgacggt aaagaggttt aaaaagaggt ttaaaaagag attataaaag agggggacaa
     2941 aatttcccgc ctaaaaaacc acttcttttt tctccaaaca gattcgcgca tttttctcaa
     3001 ctgggaatct tactccctac agtcgcttaa aaaggtgtga tcgctctgct ctcacattat
     3061 actgcgcagt cgcttccggc tcctttgcta aaaatgttca taaggaactc tttgagaagc
     3121 tatgagcctt cgtttgagac gttttaagac agtcttagta taaatccctt atcttgttct
     3181 taaagcgcct tacaggcggt cagggcaagc cctaacaacc ctaaagttgt ctgaatgccc
     3241 tctaaaattc gatttaagca ctcttaaacc tactcttata tatttatata cttagacgtt
     3301 tcaaacgtct taaaacgcaa attagagctt gataaagata tttgctcgtg tgccgacttt
     3361 tttaaaagtc taggcacacg ccatgacaaa gctgaaataa actgcttcag ctttgtcatg
     3421 gcggattcgc tcacttgaaa aagtgttcat ttcgctcgat tattagcata tattttttaa
     3481 ctttttttgc tagccaaatt ttgcattagt tcgctcataa atcccaccct ttcagtacgt
     3541 cctacggacg tatcgttcgt aatgtgaaag tacctttaaa ggtactttct acaaaactca
     3601 ctaaagtgtt tggcaagctc tatatttgct agccaaatac ctacgtattt ggtgcaaata
     3661 accgcttgtc aaaggggcgc aagctccccc ttgaatcccc ctcaaaaacc acgctccaca
     3721 atacaaaaaa ggctagcaaa tgctagccac tttttgtaaa gtttcgcttg tttcaccttc
     3781 ggtgaaatta ttgttttgta atatcaaaca actattcatc aatcagcttt aaaaaattca
     3841 attggtttag ctgtcccttc tgtcccatca ttcgttgtca aaataaatac gagctttttt
     3901 ggagaatcca aactaaaagt gtaatctaat tgttttttat tagagcgcag tgttagtaaa
     3961 ttatttgttg ctttgtaaat gaaaacttgt tcatttatat ttaaattttt atcaatgatt
     4021 tgaattttat gattgttctc ttttactaag atggtatatt gttctatatt ttttgaagta
     4081 tttaaattag tgattactag acgttctgaa tgatctttag tttgccaagt tcctagaaaa
     4141 ggaatcagat cagtcttttg tatttcttgt ttttttgaac aactagttaa gaaaagaact
     4201 aacactagac ccataactaa agcaatgggt ttatttacca atttgtaatg gtattgtccc
     4261 aagtaaaacc acctccaggt acaggaattg taactggtcc tgcatttgca cttattacag
     4321 ttgggtagtt ataccaagga ttccacacat agtaagtttg ataaccacct tgattgaccc
     4381 acccaaataa tgcaagtgca tgacggctgt tactataatt tccataacca gcagttccca
     4441 tataaatggc atttcctttt cttagttgtg tttggacctc tgaaaggttt agtattcgtg
     4501 ctactttttt aggataaaca ccacagtaat ttgcatattt tattatttga tcttgattaa
     4561 gactttttga ttcaatttga ctattccaat catcaaggaa aagaggagac ctagataaat
     4621 tttctttttg ggtttcaaaa taaccacgcc tttgtgttaa atttaagtgg tatacaccta
     4681 taattatata attaaaatat acaatatgcg ataacaaatt tttgttatag ttcataaaat
     4741 attgttttgg gaacataatc gttatttatc gagctagtta acataatgta cactatgcca
     4801 agtagcccct ctaccaatgg atagagacgc tcactttgtg atttttaatt ggtctagcta
     4861 aaagcaactt tctaaagctt gatttttcgc ggttatctat atctaattct ttgtgcattt
     4921 ttatctgtca tttttttaag acttgttatt tttgagagga tttgatatgc tttttttgtc
     4981 tttctctagt tcttcacttt gtaatatttt tatgttgatg aataggataa tctcattttg
     5041 ggctgctcgt gagagcagtc tttttttgtt tcagggtacg tcctattctt tggatttccg
     5101 cgctattaaa taaccgatac ttggggattg gggtagcgcc ccaaaataca aaaagaagaa
     5161 gttgcataag cggatttttc ttttttttcg caagttggta ccaacttgca aatcttgctg
     5221 gaagactgat ccctaaaaag aaatcgagga atttttttag ggatcggttt ctttttttat
     5281 ctttttagaa agtttgttgg gtcaaaattt ccagaagtaa aggtttgatt ggtttctttt
     5341 tgacgatttt tgggttgatt tcgtcgggaa ggattgagtt gattttcaat tttttctttc
     5401 acttcttgat acactttaga atttgtaccg ataagaacac cagtatcttt tagatataaa
     5461 tcagcaaatt ctgccatgat ttctgtcatg atagaaattt cttttcccat ggcattgagt
     5521 tttgcttggt tactggttgt ggttgtattg gtatcaggtt cataactcat acataaaaaa
     5581 cgaatggctt ctgaataatt gttgacgtct ggattttttt cgatgatgga atctaaaacg
     5641 tcaacgtgac tgggataaag ggttacgtgt ttttgaacag aagtttttct catgttatat
     5701 tcctccgata agtgtttcaa tgagttggtt gtttgtgtga acatagtcat tcagtatgtt
     5761 cgtattttct gtttgaatgg caataaattt ttcgagtaat aaatcgaatt gatctttttt
     5821 gtaattcata agttgacggt caaaatcact ttcgattaga acttttagat atttatttcg
     5881 actccatttt ttgcccgttt tggttgtgag ttcctgacaa cgtttatcta ttcttgcaat
     5941 aattttggga ttgatatttc gaactaaaat gtccatggaa attttccttt cgtatcagcg
     6001 tataatttat gtataccata caaatatagt atttgtatag ctatttttcg ccaaaacact
     6061 atacatagcc tagccacaat ggcaaaaaag tgatagcaaa tttttttctg agaaacgtga
     6121 taggcacagc ctatctagca cctttcgtaa gaaagtgata ccacgtttcc ttatgctctt
     6181 tcaaaattca gaaaaatcag ctgtaaaacg agtgcagtcc gagtaagatt caacgaaaaa
     6241 cggggattgg ggcgccacca aaatatcgca acatgggtcc atgttgctct gctcctaaaa
     6301 gcaaaagaag aataaaacca gcaattttat taatttaaat tgctggtttt attgccagaa
     6361 ttgccaccat tttttctccg tctttgattg ttttctatcg attctgtttc ttcagaaaga
     6421 gtcgttgatt tattttccga agattcttcc gtataagtca actgtagttg ttcctgcagt
     6481 ttctcgattt gttggttggc ttgcagggtg agttgttgtt ggtggtcgag taatttttgc
     6541 atttgtttta tttgctcgtc ttttcttgta gttgcatatc tttttgattt aactgttgtt
     6601 ctaggagaga aaaggacttc taattcggga acttggttgg cgtttttgtg ggtttcggtt
     6661 ttcgtttttc gccaactagt tgttgatttg agttttcgtt ttcgttaaaa acattgattt
     6721 tatagccttt tgtccggttt cattaatcac aaacttgttt ccgattttcg aaaaccaaag
     6781 ttttcgattt tcgtcagaaa cttttttgtt aattgctgtt ttactaactc ctaattcttc
     6841 agcaagttct ttgattgttt ttctgtcata aaatctacta attcaatctt tgaataagac
     6901 aaaagattgt agataaacag tcttttagtt ctagataaac atcaaattta tctctgataa
     6961 aaagacattt ttctttattt tgtatttaaa agcgtcattc ctttgttgga aagttcaatt
     7021 tttttgttct ttgatttttt tggtgaaaag aggtttaaaa aggcatgttt taaaaaaaga
     7081 gattaggttt aaatgtaaat tgaggccaac aaaatttcca gcctaaaaaa agttaaacag
     7141 acaaaagatt tttctccaaa cagattcgcg tttttttaat tcaaaaagtc gtctttaaaa
     7201 gaaaataaag cggggggtta acagtcgctt gaattttttt ttaatcgctc tgctctcgct
     7261 aacccattat actgcgcagt cgcttttccg tattttgcta aaaatgttca taaggaactc
     7321 tttgagaagc tatgagcctt cgtttgagac gttttaagac gtatttggtg caaataactg
     7381 cttgtcaaag gggcgcaagc tccccttgaa tcccctcaaa aaccacgctc cataatacaa
     7441 aaaggctagc aaatgctagc cactttttgt aaagtttcgc tttgtttcac cttcggtgaa
     7501 attattgttt tgtaatatca aacaactatt catcaatcag cttttaaaaa ttcaattggt
     7561 ttagctgttt cccttctgtc ccatcattcg ttgtcaaaat aaatacgagc tttttggaga
     7621 atccaaacta aaagtgtaat ctaattgttt ttattagagc gcagtgttag taaattattt
     7681 gttgctttgt aaatgaaaac ttgttcattt atatttaaat ttttatcaat gatttgaatt
     7741 ttatgattgt tctcttttac taagatggta tattgttcta tatatttttt gaagtattta
     7801 aattagtgat tactagacgt tctgaatgat ctttagtttg ccaagttcct agaaaaggaa
     7861 tcagatcagt cttttgtatt tttttttttg aacaactagt taagaaaaga actaacacta
     7921 gacccataac taaagcaatg ggtttattta ccaatttgta atggtattgt cccaagtaaa
     7981 accacctcca ggtacaggaa ttgtaactgg tcctgcattt gcacttatta cagttgggta
     8041 gttataccaa ggattccaca catagtaagt ttgataacca ccttgattga cccacccaaa
     8101 taatgcaagt gcatgacggc tgttactata atttccataa ccagcagttc ccatataaat
     8161 ggcatttcct tttcttagtt gtgtttggac ctctgaaagg tttagtattc gtgctacttt
     8221 ttaggataaa caccacagta atttgcatat tttattattt gatcttgatt aagacttttt
     8281 gattcaattt gactattcca atcatcaagg aaaagaggag acctagataa attttctttt
     8341 tgggtttcaa aataaccacg cctttgtgtt aaatttaagt ggtatacacc tataattata
     8401 taattaaaat atacaatatg cgataacaaa tttttgttat agttcataaa atattgtttt
     8461 gggaacataa tcgttattta tcgagctagt taacataatg tacactatgc caagtagccc
     8521 ctctaccaat ggatagagac gctcactttg tgatttttaa ttggtctagc taaaagcaac
     8581 tttctaaagc ttgatttttc gcggttatct atatctaatt ctttgtgcat ttttatctgt
     8641 cattttttaa gacttgttat ttttgagagg atttgatatg cttttttttt gtctttctct
     8701 agttcttcac tttgtaatat ttttatgttg atgaatagga taatctcatt ttgggctgct
     8761 cgtgagagca gtcttttttg tttcagggta cgtcctattc ttggatttcc gcgctattaa
     8821 ataaccgata cttggggatt ggggtagcgc cccaaaatac aaaaaaagaa gaagttgcat
     8881 aagcggattt tttttttttt ttcgcaagtt ggtaccaact tgcaaatctt gctggaagac
     8941 tgatccctaa aaagaaatcg aggaattttt tagggatcgg gtttttttat ctttttagaa
     9001 agtttgttgg gtcaaaattt ccagaagtaa aggtttgatt ggtttctttt tgacgatttt
     9061 tgggttgatt tcgtcgggaa ggattgagtt gattttcaat ttttctttca cttcttgata
     9121 cactttagaa tttgtaccga taagaacacc agtatctttt agatataaat cagcaaattc
     9181 tgccatgatt tctgtcatga tagaaatttc ttttcccatg gcattgagtt ttgcttggtt
     9241 actggttgtg gttgtattgg tatcaggttc ataactcata cataaaaacg aatggcttct
     9301 gaataattgt tgacgtctgg atttttttcg atgatggaat ctaaaacgtc aacgtgactg
     9361 ggataaaggg ttacgtgttt ttgaacagaa gtttttctca tgttatattc ctccgataag
     9421 tgtttcaatg agttggttgt ttgtgtgaac atagtcattc agtatgttcg tattttctgt
     9481 ttgaatggca ataaattttt cgagtaataa atcgaattga tctttttgta attcataagt
     9541 tgacggtcaa aatcactttc gattagaact tttagatatt tatttcgact ccattttttt
     9601 tgcccgtttt ggttgtgagt tcctgacaac gtttatctat tcttgcaata attttgggat
     9661 tgatatttcg aactaaaatg tccatggaaa ttttcctttc gtatcagcgt ataatttatg
     9721 tataccatac aaatatagta tttgtatagc tatttttcgc caaaacacta tacatagcct
     9781 agccacaatg gcaaaaagtg atagcaaatt ttttctgaga aacgtgatag gcacagccta
     9841 tctagcacct ttcgtaagaa agtgatacca cgtttcctta tgctctttca aaattcagaa
     9901 aaatcagctg taaaacgagt gcagtccgag taagattcaa cgaaaaacgg ggattggggc
     9961 gccaccaaaa tatcgcaaca tgggtccatg ttgctctgct cgcaaaaagc aaaagaagaa
    10021 taaaaccagc aatttttatt aatttaaatt gctggtttta ttgccagaat tgccaccatt
    10081 ttttccaatt ctttgtttgt tcgtccattg tttctgttgc ttcagaaaga gtcgttgaac
    10141 tattctctga agactcttcc gtataagtca actgcagttg ctcctgaagt ttttcgattt
    10201 gttggttggc ttgcagggtg agttgttgtt gctggtcgag tagtttttgt atatgtttta
    10261 tttgctcgtc tttttcttgt agttgcatat ctttttgatt taactgttgt tctaggagag
    10321 aaaggacttc taattcggga acttggttgg cgtttttgtg ggtttcggtt ttcgtttttt
    10381 cgccaactag ttgttgattt gagttttcgt tttcgttaaa aacattgatt ttatagcctt
    10441 ttgtccggtt tcattaatca caaacttgtt tccgattttc gaaaaccaaa gttttcgatt
    10501 ttcgtcagaa actttttgtt aattgctgtt ttactaactc ctaattcttc agcaagttct
    10561 ttgattgttt tttctgtcat aaaatctaaa aacccctcaa tctttgaata agttttgaag
    10621 attgtctaat aaaatttttt ttagttctga aggaatatcg gaatttatct ctgatttttg
    10681 tgcattattt tctttatttt gggtattttg ttctctttct aacttttcta actgttgtaa
    10741 aagttccaat tttcgctttt gttctttgat ttttttcttg taatgaagta ggtgcttttt
    10801 cagattgaac ttgttggtta gctagttcta atgctttatt ttggtcttct ttggttggtg
    10861 catgtttgtt aaagtcgtct gcttttttga tctcaggttt gaacgtaaat tgatagccaa
    10921 taacaggttt tccccgtcct tttccgtatt tctttttgat tgttagtcct ttgaagattg
    10981 cagttagttc ttgacgtata ggttttataa ctcgtttatc tatatcaggt tgcttataac
    11041 ttttaggaat atctaataat tcaagaaagt cttctttgga gaaatatgcg aaacctttgg
    11101 ttctgtattg ctttaacaga cgaaacatag tctttgcata gccactttgt agctcgttga
    11161 attgttgtaa agaatagcgt acccactcgt ctaaattatt aagtaagggt aaggcttttt
    11221 catgaacttg tatttcagca aaaggaatat cagattttcc gtcgatttta aattgattaa
    11281 ataaaacaaa acgttcacgt tgcaaaccgt cagcacttct acgaccaaag cgtaaagata
    11341 agattttttc ataagttgct tttaaatctt ctacaaatcg tacatttgca gttgctttgt
    11401 agttacttaa atcttttagt tggtcaaacg taaaacgaac tgtcttatcc cctttttcac
    11461 gcattctaga cacgatggaa aaaaacaaat tcatttcaac cggtgtaaat tttcttaaag
    11521 gaattgtatt taattcatgg tggtatttta caatctcatt agccatattt aaaatagctc
    11581 ctttcttact cgcacaagag atacacgtta tgaaatacaa aatgcctgtt tatctacatc
    11641 ttatattaac atcgtcgatg tactaattca atgttcaaac agacaaaatg tccttcataa
    11701 acagacaaaa tgtccttcat aaacagacaa aatgtccttc ataaacagac aaaatgtcct
    11761 tcataacatt ctgtatccat tggtagagta aggacacagc ttacggtaaa gaggtttaaa
    11821 aagaggttta aaaagagata taaaaagagg ggggctaatt ttcccgccta aaaaccactt
    11881 cttttttctt taaacaggtt cgcgcatttt tcaaaactgc caatccaaaa ccttgcttcc
    11941 tacggtcgcc aaaaaggtgt gatcgctacg ctctcacatt gtactgcgca gtcgcctaaa
    12001 ggctcctttg ctaaaaacat gttggtaaag aggcctttga gcggttctga atgcttatct
    12061 aagccgtttt aagacagtct tggataaaaa tccttatcta gctttaaagc gccttacagg
    12121 cggtcagggc aagccctaac aaccctaaag ttgtctgaat gccctctaaa attcgattta
    12181 agcactctta aacctactct tatatattta tatacttaga cgtttcaaac gtcttaaaac
    12241 gcaaattaga gcttgataaa gatatttgct cgtgtgccga cttttttaaa agtctaggca
    12301 cacgccatga caaagctgaa ataaactgct tcagctttgt catggcggat tcgctcactt
    12361 gaaaaagtgt tcatttcgct cgattattag catatatttt ttaacttttt ttgctagcca
    12421 aattttgcat tagttcgctc ataaatccca ccctttcagt acgtcctacg gacgtatcgt
    12481 tcgtaatgtg aaagtacctt taaaggtact ttctacaaaa ctcactaaag tgtttggcaa
    12541 gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct tgtcaaaggg
    12601 gcgcaagctc cccttgaatc cccctcaaaa ccacgctcca caatacaaaa aaggctagca
    12661 aatgctagcc actttttttg taaaagtttc gcttgtttca ccttcggtga aattattgtt
    12721 ttgtaatatc aaacaactat tcatcaatca gctttaaaaa ttcaattggt ttagctgtcc
    12781 cttctgtccc atcattcgtt gtcaaaataa atacgagctt ttttggagaa tccaaactaa
    12841 aagtgtaatc taattgtttt ttattagagc gcagtgttag taaattattt gttgctttgt
    12901 aaatgaaaac ttgttcattt atatttaaat ttttatcaat gatttgaatt ttatgattgt
    12961 tctcttttac taagatggta tattgttcta tattttttga agtatttaaa ttagtgatta
    13021 ctagacgttc tgaatgatct ttagtttgcc aagttcctag aaaaggaatc agatcagtct
    13081 tttgtatttc ttgttttttt gaacaactag ttaagaaaag aactaacact agacccataa
    13141 ctaaagcaat gggtttattt accaatttgt aatggtattg tcccaagtaa aaccacctcc
    13201 aggtacagga attgtaactg gtcctgcatt tgcacttatt acagttgggt agttatacca
    13261 aggattccac acatagtaag tttgataacc accttgattg acccacccaa ataatgcaag
    13321 tgcatgacgg ctgttactat aatttccata accagcagtt cccatataaa tggcatttcc
    13381 ttttcttagt tgtgtttgga cctctgaaag gtttagtatt cgtgctactt tttaggataa
    13441 acaccacagt aatttgcata ttttattatt tgatcttgat taagactttt tgattcaatt
    13501 tgactattcc aatcatcaag gaaaagagga gacctagata aattttcttt ttgggtttca
    13561 aaataaccac gcctttgtgt taaatttaag tggtatacac ctataattat ataattaaaa
    13621 tatacaatat gcgataacaa atttttgtta tagttcataa aatattgttt tgggaacata
    13681 atcgttattt atcgagctag ttaacataat gtacactatg ccaagtagcc cctctaccaa
    13741 tggatagaga cgctcacttt gtgattttta attggtctag ctaaaagcaa ctttctaaag
    13801 cttgattttt tcgcggttat ctatatctaa ttctttgtgc atttttatct gtcatttttt
    13861 taagacttgt tatttttgag aggatttgat atgctttttg tctttctcta gttcttcact
    13921 ttgtaatatt tttatgttga tgaataggat aatctcattt tgggctgctc gtgagagcag
    13981 tcttttttgt ttcagggtac gtcctattct ttggatttcc gcgctattaa ataaccgata
    14041 cttggggatt ggggtagcgc cccaaaatat aaaaaagaag aagttgcata agcggatttt
    14101 tttttttttt tttcgcaagt tggtaccaac ttgcaa
//
