LOCUS       NZ_CP041252             3336 bp    DNA     circular CON 03-DEC-2023
DEFINITION  Klebsiella electrica strain DSM 102253 plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP041252
VERSION     NZ_CP041252.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12125603
            Assembly: GCF_006711645.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella electrica
  ORGANISM  Klebsiella electrica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Thiel,S., Bunk,B., Sproeer,C., Overmann,J., Jahn,D. and
            Biedendieck,R.
  TITLE     Complete genome sequence of Raoultella electrica 1GB (DSM 102253T),
            isolated from anodic biofilms of a glucose-fed microbial fuel cell
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Thiel,S., Bunk,B., Sproeer,C., Overmann,J., Jahn,D. and
            Biedendieck,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JUN-2019) Bioinformatics, Leibniz Institute DSMZ,
            Inhoffenstr. 7B, Braunschweig 38124, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP SMRTPortal v. 2.3.0
            Assembly Name :: Raoultella_electrica_DSM 102253_HGAP3_10Mb_1SC
            Genome Coverage                   :: 130x
            Sequencing Technology             :: PacBio RSII; Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/03/2023 13:06:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,597
            CDSs (total)                      :: 5,481
            Genes (coding)                    :: 5,297
            CDSs (with protein)               :: 5,297
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 66 of 184
            Pseudo Genes (incomplete)         :: 141 of 184
            Pseudo Genes (internal stop)      :: 27 of 184
            Pseudo Genes (multiple problems)  :: 45 of 184
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP041252.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Klebsiella electrica"
                     /mol_type="genomic DNA"
                     /strain="DSM 102253"
                     /isolation_source="anodic biofilm of glucose-fed microbial
                     fuel cell originally inoculated with sewage sludge"
                     /culture_collection="DSM:102253"
                     /type_material="type strain of Raoultella electrica"
                     /db_xref="taxon:1259973"
                     /plasmid="unnamed5"
                     /geo_loc_name="Japan"
                     /collection_date="2013"
     gene            join(3146..3336,1)
                     /locus_tag="Electrica_RS28310"
     CDS             join(3146..3336,1)
                     /locus_tag="Electrica_RS28310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_023302508.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1..423
                     /locus_tag="Electrica_RS28315"
     CDS             1..423
                     /locus_tag="Electrica_RS28315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_142256031.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTSINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLPGHKREPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1171..>1419)
                     /locus_tag="Electrica_RS28325"
                     /pseudo=""
     CDS             complement(1171..>1419)
                     /locus_tag="Electrica_RS28325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178052.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            <1418..1901
                     /locus_tag="Electrica_RS28330"
                     /old_locus_tag="electrica_05669"
                     /pseudo=""
     CDS             <1418..1901
                     /locus_tag="Electrica_RS28330"
                     /old_locus_tag="electrica_05669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178051.1"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
                     /name="S-type pyocin domain-containing protein"
     gene            1904..2161
                     /locus_tag="Electrica_RS28335"
                     /old_locus_tag="electrica_05670"
     CDS             1904..2161
                     /locus_tag="Electrica_RS28335"
                     /old_locus_tag="electrica_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_025714833.1"
                     /translation="MAGNKISDFTEEEFTAFVSKIIAADFPTDKENDDAIYSFAQLTEH
                     PAGWNLIFKPKVGEDSSAKGIVKTVKEWRAANGKPGFKKA"
                     /name="bacteriocin immunity protein"
     gene            2269..2424
                     /locus_tag="Electrica_RS28340"
     CDS             2269..2424
                     /locus_tag="Electrica_RS28340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008323621.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_071561715.1"
                     /translation="MKKLLFLGVVTVSVVLAACQVNNVRDTGGGAVSPSSTVTGVTLDS
                     GTNGNP"
                     /name="colicin release lysis protein"
     gene            complement(2628..2741)
                     /locus_tag="Electrica_RS29240"
                     /pseudo=""
     CDS             complement(2628..2741)
                     /locus_tag="Electrica_RS29240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     Typing          complement(2629..3098)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.664"
                     /coverage="92.0"
                     /evalue="5.84e-177"
ORIGIN
        1 atggccagag tgtctattag tgaagcggca cggcttgtta aggttagccg tcccacaatt
       61 tacaagatga ttaacagtgg gaagttaagt tacacctctg ttgtaaagca cgggaaggct
      121 attaaagtta ttgatacttc agagcttatt agagtgtttg gttctcttga tggtgtcatc
      181 gatactgtaa aatatgatgt aaaaagtgac gcagaatcta caagtattaa cagcgttggt
      241 ttacatgatt tacaacatag aatcgctcta cttgaagctg aaaacgatgg tcttaaaggg
      301 gctgttaagg ctcgagatga gcatattgat tcccttcgtc aggctatgca gcttcctggg
      361 cacaagcgcg agccttcttc accaccccat tccccgtggt ggaaattctg gaaaaaatcc
      421 tgatcctgcc atgcttgagt tttcctctcc ggccatccca gggagttcct gaaccttccc
      481 aaaaatcgcc agatctctga acgctcctca cgactaaaaa tacccacaca ctacgcaaaa
      541 ttaaaatttt gcagattcct tatttaaaac aattggttaa tgaaaaatta atgtgcagtg
      601 atgtgttaca aagtgcagat ccaagtcgtg gacgactcta tggcgaatcg tttacgaatt
      661 gcccacgatt catatgtgaa tcacgcttta ttatcatgtt gttatgatga atcgactctt
      721 gattcaattc atcaatttgg gcgttttttg ttcatggtga ctcttgattc aattcacgat
      781 tcgaatcgcc ggtgaatcgt gtggtgagtc gtccggcgac ttgccataac gtgattgccg
      841 gccgtcgatg aacccgattc ctgaacgtgc tgaaaaccgg ttttccggct gcgccggcaa
      901 cgcctcacgg ccacgcaata aattgcgtcc ccgttcaccg ttttcggatc agaagaacgc
      961 cggaaacagg acttaccctg gctgaatggc gtgaatttcc gcgctatgca cttgcgcgca
     1021 tactcatgca tgccgtaaaa acagagcctg cgcgtttctg gcgggttttc gggtggtttg
     1081 ttgcctgttt taccggtttc ccgtcagaaa cgtcctgagg ccgttttagc ggtgcgtaca
     1141 attaagggat tatggtaaat ggtgacttgg ttaatcataa acatcctggg ggatggcata
     1201 caccagttgg atatccggtg catcgttgag ccatcgacct gattttttca ttaagtagcc
     1261 accaatttct tcggtagtca gccgggcatg aaacaatgct tcatcattga accagcagtc
     1321 agggtgatag atgagatcca gtgggtttgc cttgaaatta atctccagta actggagtgc
     1381 atagctgtgg tcagatgcac taccttctgc ttttcgtatg gcggatgacc tcgattttga
     1441 cgatattatt ctggtgtttc ctccggaatc cggcctaaaa ccgctgtatg tgatgtaccg
     1501 tagccctcgt aacatgcccg gtacagtgag cggcaaaggg cagaacgttg gcaataactg
     1561 gatggggggg gccagtaccg gggatggtgc tcctgttcct tcccagattg cggataaatt
     1621 acgcgggaag actttcggta gttttgattc tttccgtcgg gcgttctgga aagcggttgc
     1681 tgacgattct gcactgagca agcaacaatt agtgaagctg atattaatca gatgaaagct
     1741 ggaagggcac ctactgcgga ctttttagaa tcagtaggaa acgtgttaaa attgaattac
     1801 accacgaaaa agaaatactc caaggagggg ctgttatgga tgtcgataac attaaggcgc
     1861 taacacctaa aaatcatatt gagacacata aaggaaaata aaaatggctg gtaataaaat
     1921 ttcagatttt acggaagaag aatttactgc ttttgttagt aaaataatcg ctgctgattt
     1981 tccaactgac aaagagaacg atgatgctat ttattcgttc gcccaactaa cagagcatcc
     2041 tgctggttgg aacctcatct tcaagcctaa ggtaggcgaa gattcttcgg cgaaaggaat
     2101 tgttaaaacc gtaaaggaat ggcgagctgc taacggtaag ccaggtttta aaaaagcgtg
     2161 attgaatttg cgggttcaag ttatgagccc gctcatttgt aactctggat ttgaagaata
     2221 attactagat atccatttgc ctagaataat aaaaataaaa ggtgatatat gaaaaaatta
     2281 cttttcctag gggttgttac cgtttctgtg gttctggcag catgtcaggt caataatgtt
     2341 cgagacaccg gaggtggggc tgtttcacct tcatcgacgg tgaccggagt cacgcttgat
     2401 agcggtacga acggcaatcc gtaaaggatc ttcttgagat cccatttgga tcgtcgtaat
     2461 ctcttgctca gtaaacgaaa aaaccgcccg cagggcggtt ttctcgaagg ttctcagagc
     2521 agcaactctt tgaaccgagg taactggctt agaggagcgc agtaaccaaa ttcgttcttt
     2581 cagtttagcc ttaactggca cataacttca agactaactc ctctaaatca gttaccagtg
     2641 gctgctgcca gtggcgcttt gtcgtgtctt tccgggttgg actcaagacg atagttaccg
     2701 gattaggcgc agcagtcgga ctgaacgggg ggttcgtgca tacagtccag cttggagcga
     2761 actgccttcc cggaactgag tgtcaggcgt ggaatgagac aaacgcggcc ataacagcgg
     2821 aatgacaccg gtaaaccgaa aggcaggaac aggagagcgc acgagggagc catcaggggg
     2881 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac cactgatttg agcgtcaaat
     2941 tctgtgatgc ttgtcagggg ggcggagcct atggaaaaac ggccgctgtg ctgccttctc
     3001 cctctccgag ctgtctgctc tatgtcgtta tgtgcgcgtt gcgaccgctc gccgcagccg
     3061 aacggcagga gcgtagcgac tgagtgagcg aggaagcgga aaagagatct gtctgacatt
     3121 gagcactgac gctctgaggc cctttatgac gaaacaacag aaaacagcgc tgaacatggc
     3181 taaattcatc cagaatcagt ctctgctcct gctggagaag cttaacgagc ttgatctcga
     3241 tgttgaggcc gacctttgtg agaaactcca cgatgatgcg gagcatcttt tccgtacact
     3301 gtcctcgaga ctggatgaat tacaggatgg taattg
//
