LOCUS       NZ_CP041089             3541 bp    DNA     circular CON 08-JAN-2024
DEFINITION  Klebsiella pneumoniae strain Kp202 plasmid pKp202_7, complete
            sequence.
ACCESSION   NZ_CP041089
VERSION     NZ_CP041089.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN12082929
            Assembly: GCF_006459145.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Chen,L.
  TITLE     Genomic sequencing of K. pneumoniae strain Kp202
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Chen,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUN-2019) HMH-CDI, Hackensack-Meridian Health Center
            for Discovery and Innovation, 340 Kingsland Street, Building 102,
            Nutley, NJ 07110, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2019
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/08/2024 05:17:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,931
            CDSs (total)                      :: 5,813
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 180
            CDSs (without protein)            :: 180
            Pseudo Genes (ambiguous residues) :: 0 of 180
            Pseudo Genes (frameshifted)       :: 71 of 180
            Pseudo Genes (incomplete)         :: 104 of 180
            Pseudo Genes (internal stop)      :: 29 of 180
            Pseudo Genes (multiple problems)  :: 20 of 180
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP041089.1.
            Bacteria and source DNA available from HMH-CDI.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="Kp202"
                     /db_xref="taxon:573"
                     /plasmid="pKp202_7"
                     /collected_by="CDI"
     gene            complement(join(3488..3541,1..348))
                     /locus_tag="FJV42_RS29955"
                     /old_locus_tag="FJV42_29950"
     CDS             complement(join(3488..3541,1..348))
                     /locus_tag="FJV42_RS29955"
                     /old_locus_tag="FJV42_29950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_009310048.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(348..539)
                     /locus_tag="FJV42_RS29960"
                     /old_locus_tag="FJV42_29955"
     CDS             complement(348..539)
                     /locus_tag="FJV42_RS29960"
                     /old_locus_tag="FJV42_29955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            953..1066
                     /locus_tag="FJV42_RS30940"
                     /old_locus_tag="FJV42_29965"
     CDS             953..1066
                     /locus_tag="FJV42_RS30940"
                     /old_locus_tag="FJV42_29965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072032591.1"
                     /translation="MHEPPVQSDCCALSGNYHLESNPKRHDKAPLAAATGN"
                     /name="replication initiation protein"
     gene            1364..2257
                     /locus_tag="FJV42_RS29975"
                     /old_locus_tag="FJV42_29970"
     CDS             1364..2257
                     /locus_tag="FJV42_RS29975"
                     /old_locus_tag="FJV42_29970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="WP_032419557.1"
                     /translation="MELNLCWWNIGISPPVKGVKKDKAEAIRLAKHYIKKMSQEKTLDF
                     LGICEISEDEAIDFKPLADKLHLEYLNLSGTVGRIIMDVSIMYEASKLEYIDYKYIIKI
                     QPDDRTLRIAVRVVFKDAVTGNLITFFLSHWPSIRSADEKIREDAAESLRNNVNKILDD
                     HGIESQIICMGDYNTNPYSVAINEKLYATRDYHRIKNKRKLLFNPFWYLLSDKKTNNLG
                     TYHYKSGASNRWFVFDQMLFSSSFIYGGKDCLKLDMNSLDFHRILSDDNQCLDQRFFNT
                     FDHYPIFCRIYHDNQK"
                     /name="endonuclease"
     gene            2241..2678
                     /locus_tag="FJV42_RS29980"
                     /old_locus_tag="FJV42_29975"
     CDS             2241..2678
                     /locus_tag="FJV42_RS29980"
                     /old_locus_tag="FJV42_29975"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032419556.1"
                     /translation="MTTKNEEFQERLKASLKKANETLILKEKIESDISSILSMLKGITS
                     DSIDFDIFQNSSKIPKYKDCKKIVRIKKNSSMAYPKGFILFGFSINESTGYPVQVETED
                     EAAECTDVDNLKESIVYIIEKRSIEIMKLISEQQEDDDIPF"
                     /name="hypothetical protein"
     Typing          661..1066
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.643"
                     /coverage="99.0"
                     /evalue="4.5799999999999997e-172"
ORIGIN
        1 ctggtgattt tcaagaagga gcatggcctg tttcaggctg tcaatgtgcg aatccttgga
       61 caaaagtaag gtattcaggt gttcaatctc ctgtttcaga tgacgaatca cctgttcact
      121 ctggtcagca gaggctgtat cacgctgttc agagacctgt ccagaatggt gttcaccctt
      181 cggttgttca aagggaccaa agacacgtaa aagctctgaa gtatctacca tttttgcatt
      241 tcgttcaccg ctacaggtgg acaggtcacc tgctttaatt aacctgtgca gtgttgttct
      301 actcttaccc gtcagtcggg cagcctcact aatagacact ctggccatca gttaccatcc
      361 tgtaatgcat ccagtctcga ggacagtgta cggaaaagat gctccgcatc atcgtggagt
      421 ttctcacaaa ggtcggcctc gacatcaaga tcaagctcgt taagcttctc cagcaggagc
      481 agagactgat tctggatgaa tttagccatg ttcagcgctg ttttctgttg tttcgtcata
      541 agaagcctca gagcgtcagt gctcagtgta agaacagtct cttttccgct tcctcgctca
      601 ctcagtcgct acgctccggt cgttcggctg cggcgagcgg caggagcatg cgtataaaag
      661 ctcgagaaga ccgagcggtc aaatggggaa gaaggaaagg ccgcaaagcg gccgtttttc
      721 cataggctcc gcccccctga caagcatcac cgaagcggac gctcaaatca gtggtggcga
      781 aacccgacag gactataaag ataccaggcg tttccccctg atggctccct cgtgcgctct
      841 cctgttcctg ccattcggtt taccggtgtc attccgctgt tatggccgcg tttttctcat
      901 tccacgcctg acactcagtt ccgggtaggc agttcgctcc aagctggact gtatgcacga
      961 accccccgtt cagtccgact gctgcgcctt atccggtaac tatcatcttg agtccaaccc
     1021 gaaaagacac gacaaagcgc cactggcagc agccactggt aactgattta gaggagttag
     1081 tcttgaagtt atgggtcagt taaggctaca ctgaaagaac aagttttggt gactgcgctc
     1141 ctctactagc cagttaccta ggttcaaaag acctgcaagt tatttgaacc cttgaaaaaa
     1201 ccgccctgca aggcggtttt ttcatttcca gccaagaaga taacgcctta aaaggggtag
     1261 cgcaacacca tttatcaaca aacagataaa taaaatgttt aacccccaca tcaaaagcaa
     1321 tacaataaac ccaatgaata caatgcatta agaaagaaac accatggaat tgaatttatg
     1381 ttggtggaat attggaatat caccacctgt aaaaggcgtg aagaaagaca aggctgaggc
     1441 tattagattg gccaagcact atataaaaaa aatgtctcaa gaaaaaacat tggatttttt
     1501 gggtatctgt gaaatatcag aagatgaggc tatagatttt aaacctttgg ctgataaact
     1561 acatcttgaa tacttgaatt tatcagggac tgttggtcgg ataattatgg atgtatccat
     1621 aatgtacgaa gcatcaaaac ttgagtacat agattataaa tatataatta aaatacaacc
     1681 agatgataga actttgcgta ttgctgtcag agttgttttt aaggatgcag taacaggaaa
     1741 tctaataaca ttctttttat cacactggcc atccataaga agcgcagatg aaaagattcg
     1801 agaggacgct gccgagtcac tgcgcaacaa tgtgaataaa atactagatg accacggcat
     1861 tgagtcccaa ataatatgca tgggtgatta caacacaaac ccttactctg ttgcaataaa
     1921 cgaaaaactt tacgccacta gagattacca tcgaataaag aataaacgaa aacttctttt
     1981 taaccctttt tggtatttac tttcagacaa aaagaccaat aacttaggaa cttatcatta
     2041 taaatcagga gcatcgaata gatggtttgt ttttgatcaa atgttgtttt catcatcatt
     2101 tatatacgga ggaaaagatt gtcttaagct agacatgaac agccttgatt ttcatcgtat
     2161 acttagtgat gataaccaat gtttagatca aagatttttt aacacttttg atcattaccc
     2221 aatattttgt aggatttacc atgacaacca aaaatgaaga gttccaagaa agactaaagg
     2281 catcactaaa aaaagccaat gaaacactaa ttttaaaaga aaaaatagag tcagatattt
     2341 catcaatttt gtcaatgcta aagggtataa catccgactc aattgacttt gatatttttc
     2401 agaatagttc gaaaattccc aaatataaag attgtaaaaa aattgtcaga ataaagaaaa
     2461 actctagcat ggcataccct aaaggtttca ttttatttgg atttagcata aatgagtcta
     2521 ccggatatcc tgtacaggta gaaacggaag acgaagccgc agagtgtaca gatgtggata
     2581 acttaaaaga atcaattgtt tacataatag aaaaaagatc tatagagata atgaaattaa
     2641 tatccgagca acaagaagac gatgacattc ctttttaaaa aggtagcatg taatgccatt
     2701 atcaacggca ctttttcata gaaggtgcca tatatcttag aatcaactaa tttaacataa
     2761 gaccataatt gtacgcaccg ctaaaacggc ctcagggcgt ttctgacggg aaaccggtaa
     2821 aacaggcaac aaaccacccg aaaacccgcc agaaacgcgc aggctctgtt tttacggcat
     2881 gcatgagtat gcacgcaagt gcatagcgcg aaaattcacg ccattcagcc agggtaagtc
     2941 ctgtttccgg cgttcttccc atccgaaaac ggtgaacggg gacgcaattt attgcgtggc
     3001 cgtgaagcgt tgccggcgca gccggaaaac cggttttcag cacgttcagg aatcgggttc
     3061 gtcgacggcc ggcaatcacg ttatggcaag tcgccggacg actcaccaca cgattcaccg
     3121 gcgactcttc actagagtcg tacccagagt cgccgcgtct aaaaaacaac caaacgggga
     3181 gtcgtgtcca gagtcgggtc gttattaact cattgttttt atagtcctga gtcgttaccg
     3241 actctatgac gactcgccgg ggactctatg acgagtcgtc atagagtcgg atctatgtaa
     3301 ataaaattta aaaaacgaaa tttcaggaaa gtaaacaact gatattaaat acaaatctgc
     3361 aaaattttaa ttttgcgtag tgtgtgggta tttttagtcg tgaggagcgt tcagagatct
     3421 ggcgattttt gggaaggttc aggaactccc cgggatggcc ggagaggaaa actcaagcat
     3481 ggcaggatca ggattttttc cagaatttcc accatggtgc cggggattcc gttaattttg
     3541 c
//
