LOCUS       NZ_CP040891             9782 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Leclercia adecarboxylata strain Z96-1 plasmid pMCR-Z96-1, complete
            sequence.
ACCESSION   NZ_CP040891
VERSION     NZ_CP040891.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN11950933
            Assembly: GCF_006171285.1
KEYWORDS    RefSeq.
SOURCE      Leclercia adecarboxylata
  ORGANISM  Leclercia adecarboxylata
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Leclercia.
REFERENCE   1  (bases 1 to 9782)
  AUTHORS   Sun,Q., Wang,H., Shu,L., Dong,N., Yang,F., Zhou,H., Chen,S. and
            Zhang,R.
  TITLE     Intestinal colonization of mcr-4.6 and blaIMP-4 bearing Leclercia
            adcarboxglata in an inpatient in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9782)
  AUTHORS   Sun,Q., Wang,H., Shu,L., Dong,N., Yang,F., Zhou,H., Chen,S. and
            Zhang,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JUN-2019) Clinical Laboratory, Second Affiliated
            Hospital of Zhejiang University, School of Medicine, 88 Jiefang
            Road, Hangzhou, Zhejiang 310009, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.3.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 14:18:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,565
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,267
            CDSs (with protein)               :: 5,267
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 74 of 177
            Pseudo Genes (incomplete)         :: 106 of 177
            Pseudo Genes (internal stop)      :: 31 of 177
            Pseudo Genes (multiple problems)  :: 31 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP040891.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9782
                     /organism="Leclercia adecarboxylata"
                     /mol_type="genomic DNA"
                     /strain="Z96-1"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:83655"
                     /plasmid="pMCR-Z96-1"
                     /geo_loc_name="China: Hangzhou"
                     /collection_date="2018-11"
     gene            1..951
                     /locus_tag="FHN83_RS26225"
                     /old_locus_tag="FHN83_26225"
     CDS             1..951
                     /locus_tag="FHN83_RS26225"
                     /old_locus_tag="FHN83_26225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946646.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_034167667.1"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSHLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
                     /name="replication initiation protein"
     gene            1031..2118
                     /locus_tag="FHN83_RS26230"
                     /old_locus_tag="FHN83_26230"
     CDS             join(1031..1283,1283..2118)
                     /locus_tag="FHN83_RS26230"
                     /old_locus_tag="FHN83_26230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611587.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="WP_088569307.1"
                     /translation="MKKSRFTDSQIMTILKQAEAGTPVPELCREHGFSSASFYKWRSKF
                     GGMDASLMARLKELEDENRRLKKMYAEERLKAEVIQEAMFKKVVRPSQRRQMARHAVST
                     QQISIRLACQIFSVSETCYRYLPRLSVENQRIAGWLLRITGSQRNWGFGLCFLYLRNVK
                     GFRWNHKRVYRIYCELSLNMRIKPKKRLKRDKPEPLAVPEYSNECWSMDFMHDQLSDGR
                     SVRLLNIIDDFNREALAIEVDFSLPSSRVKRTLEQIIEWRGKPAAIRCDNGPEYTSHEL
                     INWAEDNGIKLNFIQPGNPQQNAYIERYNRTVRYDWLGQYLFCSLDELQRYATEWQWFY
                     NHERPNMALGGYTPRQHELRTA"
                     /name="IS3 family transposase"
     gene            2160..2573
                     /locus_tag="FHN83_RS26235"
                     /old_locus_tag="FHN83_26235"
     CDS             2160..2573
                     /locus_tag="FHN83_RS26235"
                     /old_locus_tag="FHN83_26235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001229901.1"
                     /translation="MALVSISEASRLTNKSRTTVHRYISKGKLSICTDENGVKKIDTSE
                     LLRVFGAFKAVHHEQIDNVTDEQHVTPNRTGETSKTKQLEHEIEHLRQLVTAQQSHIDS
                     LKQAMLLIESKLPTTREPVTPPVGKKSWQFWKK"
                     /name="hypothetical protein"
     gene            2820..3137
                     /locus_tag="FHN83_RS26240"
                     /old_locus_tag="FHN83_26240"
     CDS             2820..3137
                     /locus_tag="FHN83_RS26240"
                     /old_locus_tag="FHN83_26240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004142563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_040113353.1"
                     /translation="MFNVILHDDVPNELAGLPPVVRAKMIRLIDKLKTNATALREPDSK
                     PLRDGLFELRTMGTDIARGLYVYQKGRNIYLLRVFIKKTQKTPSGEISMAFERLKEMLN
                     E"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            3130..3417
                     /locus_tag="FHN83_RS26245"
                     /old_locus_tag="FHN83_26245"
     CDS             3130..3417
                     /locus_tag="FHN83_RS26245"
                     /old_locus_tag="FHN83_26245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012104592.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_172691904.1"
                     /translation="MSKVKGIAWEHVRNELMSNPEVQAAYEAEERKERLQAMLAEWRHH
                     AGLTRAQVAERMGVTPPTVSRMESNVIKASIETLARYARACGVKHPQITL"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(3755..5380)
                     /locus_tag="FHN83_RS26250"
                     /old_locus_tag="FHN83_26250"
     CDS             complement(3755..5380)
                     /locus_tag="FHN83_RS26250"
                     /old_locus_tag="FHN83_26250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006081694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine--lipid A transferase MCR-4.3"
                     /protein_id="WP_011638903.1"
                     /translation="MISRFKTLSVNQFTFITALFYVAIFNLPLFGIVRKGIEKQPEVDP
                     LFIASMPLFLTFALSFLFSIFTVKYLLKPFFIVLTLLSSSVFFAAYQYNVVFDYGMIEN
                     TFQTHPAEALMYVNLASITNLLLTGLLPSYLIYKADIHYQPFFKELLHKLAFMLLMFVG
                     IGIVAFFYYQDYAAFGRNNSELRRYIVPTYFVSSASKYLNEHYLQTPMEYQQLGLDAKN
                     ASRNPNTKPNLLVFVVGETARSMSYQYYGYNKPTNAHTQNQGLIAFNDTSSCGTATAVS
                     LPCMFSRMGRADYDPRRANAQDTVIDVLSHSGIKVQWFDNDSGCKGVCDQVENLTIDLK
                     SDPKLCSGQYCFDQVLLNKLDKILAVAPSQDTVIFLHIIGSHGPTYYLRYPPEHRKFIP
                     DCPRSDIQNCSQEELINTYDNTILYTDFILSEVVNKLKGKQDMFDTAMLYLSDHGESLG
                     EKGMYLHGAPYSIAPKEQTSVPMLAWVSNDFSQDNQLNMTCVAQRAEQGGFSHDNLFDS
                     LLGLMNVKTTVYQSQLDIFAPCRY"
                     /name="phosphoethanolamine--lipid A transferase MCR-4.3"
     gene            5604..>5915
                     /locus_tag="FHN83_RS26255"
                     /old_locus_tag="FHN83_26255"
                     /pseudo=""
     CDS             5604..>5915
                     /locus_tag="FHN83_RS26255"
                     /old_locus_tag="FHN83_26255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019445.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /name="IS5/IS1182 family transposase"
     gene            complement(<5888..5962)
                     /locus_tag="FHN83_RS26260"
                     /old_locus_tag="FHN83_26260"
                     /pseudo=""
     CDS             complement(<5888..5962)
                     /locus_tag="FHN83_RS26260"
                     /old_locus_tag="FHN83_26260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014654019.1"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /name="IS1 family transposase"
     gene            6260..6823
                     /locus_tag="FHN83_RS26270"
                     /old_locus_tag="FHN83_26270"
     CDS             6260..6823
                     /locus_tag="FHN83_RS26270"
                     /old_locus_tag="FHN83_26270"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_255296771.1"
                     /translation="MHHWPSNPIKDYSLHIDPSVIINQKITAAPTIGKRWYVYDNAILN
                     QLEHRLVKTLEGFMPKLRARYDDIYVLRNDEQSTRFKLTEFGGVRGFMPDFIMILTRHA
                     DNAYWQVFLEPKGDDRLLDDAWKERMLETLNDRERIVIDENEDVRLVGIKFFANSQMDA
                     FVSDMQNRLNEGESLETASLSLPL"
                     /name="hypothetical protein"
     gene            complement(6862..7446)
                     /locus_tag="FHN83_RS26275"
                     /old_locus_tag="FHN83_26275"
     CDS             complement(6862..7446)
                     /locus_tag="FHN83_RS26275"
                     /old_locus_tag="FHN83_26275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobX"
                     /protein_id="WP_040126191.1"
                     /translation="MSDDIKQIAALIGHHQALEKRVTSLTEQFQDASAQLQQQSDTLSR
                     LIRELDSASGNMTETVRQSVHAALIQVEKELKQAGLAQQKPATEALNQATDTAKAMIHE
                     MRREMSRYTWKSAIYLVLTIFFVMVACVTAFTWFMNDGYSQIAEMQRMEAVWQKKAPLA
                     DISRCDGKPCVKVDTRATYGDKENTWMIIKK"
                     /name="mobilization protein MobX"
     gene            complement(7459..8556)
                     /gene="mobA"
                     /locus_tag="FHN83_RS26280"
                     /old_locus_tag="FHN83_26280"
     CDS             complement(7459..8556)
                     /gene="mobA"
                     /locus_tag="FHN83_RS26280"
                     /old_locus_tag="FHN83_26280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobA"
                     /protein_id="WP_040113349.1"
                     /translation="MASYHLSVKTGGKGSASPHADYISREGKYAREKDSDLEHKESGNM
                     PAWAAHKPTEFWKAADTFERANGCTYREIEIALPRELKPEQRLELVRDFVRQEIGDRHA
                     YQFAIHNPKAAIAGGEQPHAHIMFSERMNDGIDRDPQQYFKRANSKAPERGGAKKARFG
                     ETPTERKEHLVAQRERWADLQNKHLERYQHTDRVDARSLKAQGIEREPERHLGAGQVQR
                     FDTAQLQAILERRDAERQAEQSRDERDSVIDVTTSLREAISERDNLTIKQEQKPEPAQE
                     DTSGRVFDFEKEPEKLNALVSDALKDVQDEIDLQSLVNETMAEFQGIHQEMERLAEKQR
                     QQEKEQQRLAEQTRQKPDKGWSFSR"
                     /name="mobA"
     gene            8721..9188
                     /gene="traD"
                     /locus_tag="FHN83_RS26285"
                     /old_locus_tag="FHN83_26285"
     CDS             8721..9188
                     /gene="traD"
                     /locus_tag="FHN83_RS26285"
                     /old_locus_tag="FHN83_26285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_064645523.1"
                     /translation="MNKSEKQEKWAADRVQYIRGLKSPNEQQKLMLILTDKADKTAQDI
                     KTLSLLMKAEQAAEKAQEARAKVMNLIQAEKRAEARAARKARDHALYQSAGLLILAGLV
                     DSQTGKPVDDTAALLGALASLNDLSRDNPKWSDWKIRGQELLNSKKSDSSA"
                     /name="traD"
     ARG             complement(3759..5380)
                     /name="mcr-4.3"
                     /gene="mcr-4.3"
                     /gene_name="phosphoethanolamine--lipid A transferase
                     MCR-4.3"
                     /tool="amrfinderplus"
                     /drug_class="COLISTIN"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          776..951
                     /name="ColE10"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.01e-87"
     Typing          complement(7463..8556)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgtctgaat tagttgtttt caaagcaaat gaactagcga ttagtcgcta tgacttaacg
       61 gagcatgaaa ccaagctaat tttatgctgt gtggcactac tcaaccccac gattgaaaac
      121 cctacaagga aagaacggac ggtatcgttc acttataacc aatacgctca gatgatgaac
      181 atcagtaggg aaaatgctta tggtgtatta gctaaagcaa ccagagagct gatgacgaga
      241 actgtggaaa tcaggaatcc tttggttaaa ggctttgaga ttttccagtg gacaaactat
      301 gccaagttct caagcgaaaa attagaatta gtttttagtg aagagatatt gccttatctt
      361 ttccagttaa aaaaattcat aaaatataat ctggaacatg ttaagtcttt tgaaaacaaa
      421 tactctatga ggatttatga gtggttatta aaagaactaa cacaaaagaa aactcacaag
      481 gcaaatatag agattagcct tgatgaattt aagttcatgt taatgcttga aaataactac
      541 catgagttta aaaggcttaa ccaatgggtt ttgaaaccaa taagtaaaga tttaaacact
      601 tacagcaata tgaaattggt ggttgataag cgaggccgcc cgactgatac gttgattttc
      661 caagttgaac tagatagaca aatggatctc gtaaccgaac ttgagaacaa ccagataaaa
      721 atgaatggtg acaaaatacc aacaaccatt acatcagatt cccacctaca taacggacta
      781 agaaaaacac tacacgatgc tttaactgca aaaattcagc tcaccagttt tgaggcaaaa
      841 tttttgagtg acatgcaaag taagtatgat ctcaatggtt cgttctcatg gctcacgcaa
      901 aaacaacgaa ccacactaga gaacatactg gctaaatacg gaaggatctg aggttcttat
      961 ggtaatcctc ccgaaaaagc agtgtgttca taagtagaat tttctcgtaa tctcaactga
     1021 ggagatctct atgaaaaaat cacgtttcac cgacagccaa atcatgacca ttctgaaaca
     1081 ggctgaagcc ggaacaccgg tccctgagct gtgccgggaa catggcttca gcagcgccag
     1141 cttctataaa tggcggtcaa agtttggcgg gatggatgcc tctctgatgg cgcgtctcaa
     1201 agaactggaa gatgaaaatc ggcgtcttaa gaaaatgtat gccgaagagc gacttaaagc
     1261 tgaagttatt caggaagcta tgtcaaaaaa gtggtgaggc catctcaacg ccgacagatg
     1321 gccagacacg ccgtcagcac gcagcaaatc agtatccgcc tggcctgcca gattttttct
     1381 gtcagtgaaa cgtgctatcg atatctgcca cgtctttctg tagaaaatca gcgtattgct
     1441 ggctggctac tgcgcattac aggcagccag cggaactggg gttttggttt gtgctttttg
     1501 tacctgcgta atgtgaaagg ttttcgctgg aaccataaac gcgtttatcg aatttactgc
     1561 gaattatcac tgaatatgcg gatcaaacct aaaaagaggc tgaagcgtga taagccagag
     1621 ccgcttgcgg tgccggaata cagcaacgaa tgctggtcaa tggacttcat gcatgatcag
     1681 ctttcagatg gccgttcagt tcggcttttg aacattattg atgacttcaa ccgggaagcc
     1741 ctggcaatag aagtggactt ctcgctgccg tccagtcggg taaaacgcac acttgagcag
     1801 atcattgaat ggcgaggtaa acccgccgca atcagatgtg acaacggccc ggaatacacg
     1861 agccacgaat taataaactg ggcggaggat aacggaataa aactcaactt tattcagccg
     1921 ggaaatccgc aacaaaacgc ttatattgag cgctataacc ggacagtgcg ttatgactgg
     1981 ctggggcagt atttattttg ttcgctggat gaattacaac gctacgcgac agaatggcag
     2041 tggttttata atcacgaaag gccgaatatg gcactgggtg gttatacgcc aagacaacat
     2101 gagctgcgca cagcctaagt tctactattg acctctgcta aaaatggggg gattacctta
     2161 tggctcttgt atctatcagt gaagcatcaa gactaacaaa caaaagtaga acaactgttc
     2221 accgttacat atcaaaggga aaactgtcca tatgcacaga tgaaaacggt gtaaaaaaga
     2281 tagatacatc agagctttta cgagtttttg gtgcattcaa agctgttcac catgaacaga
     2341 tcgacaatgt aacagatgaa cagcatgtaa cacctaatag aacaggtgaa accagtaaaa
     2401 caaagcaact agaacatgaa attgaacacc tgagacaact tgttacagct caacagtcac
     2461 acatagacag cctgaaacag gcgatgctgc ttatcgaatc aaagctgccg acaacacggg
     2521 agccagtgac gcctcccgtg gggaaaaaat catggcaatt ctggaagaaa tagcgttttc
     2581 agccggcaaa ccgactgaaa ccggatctgc gattctgata acaagtcggc aacaccaaaa
     2641 cagccagttt gcgagcggaa aaaaacgcac ttttggacgt tccggcgggt ttcaggggtg
     2701 agtaccaggt cagtggtgcg cgcaagatcg cttatgttaa atacagcaga aaaaccataa
     2761 aattacccta taacgcaaat tgctttttat gctaatattt cgcaatggag ggcgaaccag
     2821 tgtttaacgt catattgcac gatgatgtac caaatgagct ggctggctta ccgccagtag
     2881 tcagagcgaa gatgattcgc ctgattgata aacttaaaac caacgctact gcattacgag
     2941 agccagatag caaaccactc cgggatggat tatttgagtt aaggacgatg gggacggata
     3001 ttgccagagg tctttatgtt tatcagaaag gcaggaatat ctatctactt agagtcttta
     3061 ttaaaaagac tcagaaaacc ccatcgggtg aaatatctat ggcctttgaa cgtcttaagg
     3121 agatgctaaa tgagtaaagt taaaggtatc gcttgggagc atgttaggaa tgaattgatg
     3181 tctaatcctg aggttcaagc agcctatgaa gcagaggagc gcaaggaaag acttcaagct
     3241 atgctcgctg aatggcgtca tcacgcagga ttaacaagag cccaggtggc agaacgcatg
     3301 ggtgttactc cacctaccgt ttctcgcatg gaatctaatg tgattaaggc tagcattgaa
     3361 acactggctc gatacgcaag agcatgcggt gttaagcacc ctcagatcac gttgtaattt
     3421 tcaaggccgc tttaagcagc cttttgatgc gtgcaagatc gcttatgttg aacaaggagt
     3481 ttttttgcaa aaaaccatca tttcacacat atgagataat cctaagggat ataaataggc
     3541 taattttgcc caacgcgcca aaaatgtaaa gttagcattt acagcttgta agccattgtt
     3601 ctagtttcac aacggcaaat tgatgttaag tctaaaagac cgtgataact cattgtggaa
     3661 gattgagagc taaatgtaac aatagaggac attgttagtc caagatttaa ggggtgaata
     3721 tgtcacccct aagataaaac atttacagcc atatctaata cctgcaaggt gcaaaaatat
     3781 cgagttggct ctgatagacg gtggttttta cattcataag tcctagcaaa ctgtcgaaca
     3841 aattgtcgtg ggaaaagccg ccctgttctg ctcgctgtgc aacacaagtc atgttcaact
     3901 gattatcttg gctaaagtca ttagataccc aagccagcat tggtacgcta gtttgttctt
     3961 tcggtgcaat actatagggc gcaccatgta aatacatgcc cttttcaccc aaagactcac
     4021 catggtcaga gagatacagc attgcagtat cgaacatatc ctgcttacct tttaatttat
     4081 tcaccacttc actgagaata aaatccgtat atagaatagt gttgtcgtag gtgttaatca
     4141 gttcttcttg actgcaattt tgaatatcac tgcgcggaca atccggtata aatttacgat
     4201 gctctggcgg gtatctaaga taataagttg gtccatgact accaatgata tgcaaaaaaa
     4261 ttactgtatc ttgacttggt gctactgcca gaattttatc taatttgttg agcaatactt
     4321 ggtcaaaaca atattggcca gaacacagct tcggatcact cttcaaatct atcgtgagat
     4381 tttcaacctg atcacacaca cctttacagc cagaatcatt atcaaaccac tgtactttta
     4441 taccactatg acttaacaca tcaatcactg tgtcttgagc attagcacgg cgaggatcat
     4501 agtctgcccg ccccattcgt gaaaacatac agggtagaga caccgccgtg gccgtgccgc
     4561 atgagctagt atcgttaaac gcaatcagcc cctgattttg ggtatgagca ttggttggct
     4621 tgttatatcc ataatattga tagctcattg agcgcgcagt ttcacccaca acaaacacta
     4681 ataagttagg tttagtgttc gggttacgac tggcattctt cgcatctagg ccaagttgtt
     4741 ggtattccat gggcgtctgc aaatagtgct cattgagata tttagatgca ctactgacaa
     4801 aataggtagg gacaatgtaa cgccttaact cactgttgtt tcgaccaaat gcagcataat
     4861 cttgatagta aaaaaaggcg actatcccaa tgccaacgaa cattagcagc ataaaggcta
     4921 atttatgcaa taactcctta aaaaagggct gataatgaat atcggcctta taaataagat
     4981 atgacggtaa tagcccagtc agcagtagat tggtaattga tgcaagattt acatacatca
     5041 atgcttcagc aggatgtgtt tgaaacgtgt tttctatcat gccgtagtca aacacgacat
     5101 tgtattgata ggctgcaaaa aatacacttg aggaaagtaa cgtcaatacg ataaaaaagg
     5161 gcttcagcag gtatttgacg gtaaaaattg aaaacaaaaa actcagcgca aatgttaaaa
     5221 atagcggcat agatgcgatg aaaaggggat caacttctgg ttgtttttca attccttttc
     5281 gcactatacc aaagagcggt agattgaaaa tggcaacata aaacaacgca gtgatgaaag
     5341 tgaattggtt aaccgataac gtcttaaatc tagaaatcac aaaaacccct tttttgctaa
     5401 aaaaaacaat actagcttga cctcattaaa ataaccttat gattaaaagg ttattttaat
     5461 gagtcaccaa caaacttaac agataagacc gttttaggtg aattgagccc aattcaccta
     5521 aaagttacat gtaaaggaag gtgcgaacaa gtccctgata tgagatcatg tttgtcatct
     5581 ggagccatgg aacagggttc atcatgagtc atcaacttac cttcgccgac agtgaattca
     5641 gcagtaagcg ccgtcagacc agaaaagaga ttttcttgtc ccgcatggag cagattctgc
     5701 catggcaaaa catggtggaa gtcatcgagc cgttttaccc caaggctggt aatggccggc
     5761 gaccttatcc gctggaaacc atgctacgca ttcactgcat gcagcattgg tacaacctga
     5821 gcgatggcgc gatggaagat gctctgtacg aaatcgcctc catgcgtctg tttgcccggt
     5881 tatccctgga tagcgccttg ccggaccgca ccaccccgtc agtagctgaa caggagggac
     5941 agctgataga aacagaagcc actggagcac ctcaaaaaca ccatcataca ctaaatcagt
     6001 aagttggcag catcacccat gtccgaaagt aagcaaaacc attaacggac agccggatca
     6061 tttcgtacat cccttacggt caggggttcg cgctcaatgg aacgaaatca cccgttagct
     6121 taaaacgctt aacgtaggat aatttccttt ttccaagcgg acccgtccct cagccccacc
     6181 atgacgactg aagtggctgt aaacgcagta cctatcaatt tcaatataaa caatgaacta
     6241 acgctgttag cgtggttttt tgcatcattg gccctcaaac cccattaaag actacagcct
     6301 gcacattgac ccgtcggtca ttattaacca gaaaatcacc gccgcaccca ccatcggtaa
     6361 aagatggtac gtgtacgata acgcgatact gaatcagctt gagcaccgtc tggtgaaaac
     6421 gctggaaggt tttatgccga agctcagagc acgttacgac gatatttacg tgctgcgtaa
     6481 cgacgagcag tcaacgcgct ttaagctcac ggagttcggc ggcgtgcgcg gctttatgcc
     6541 ggacttcatc atgatactga cgcgccatgc ggataatgct tactggcagg tcttccttga
     6601 gccgaaaggg gatgaccgtc tgctggatga cgcatggaaa gagcggatgc tcgaaaccct
     6661 gaatgacagg gagcgcattg tgattgatga aaatgaggac gtcaggctgg tcggaattaa
     6721 attctttgcc aacagtcaga tggacgcctt cgtcagcgac atgcaaaacc ggctgaatga
     6781 gggcgaatcg ctggaaaccg cttcgctctc cctgccgctg taattcacga caacaataac
     6841 ggcgcaatgt gcgccgttat gttatttttt gataatcatc caggtattct ctttatcacc
     6901 atacgtagct cgggtgtcca ctttaacgca gggctttccg tcacaacgcg atatatcagc
     6961 cagcggggct ttcttctgcc agaccgcttc catccgttgc atttcagcaa tctggctgta
     7021 cccgtcattc ataaaccacg taaatgccgt cacgcaggca accatgacga aaaatatcgt
     7081 cagcacgaga taaatcgcgg atttccacgt atagcgcgac atctcgcgtc gcatttcatg
     7141 gatcatcgcc ttcgccgtat cggtagcctg attaagcgct tctgtcgccg gtttctgctg
     7201 tgctaatccg gcttgtttca gctctttctc aacctgaatg agcgcggcat gaacagattg
     7261 cctgacggtt tcagtcatat taccggacgc gctgtccagc tcacgaatga gcctgctcag
     7321 cgtgtcactc tgctgctgta attgtgctga ggcgtcctga aactgctcag tcagagaggt
     7381 aacacgcttt tccagcgcct gatgatgccc gatgagggca gcgatttgtt taatgtcgtc
     7441 gctcatgaaa aatcctgtct atcgtgaaaa tgaccagcct ttatccggct tctgtcgtgt
     7501 ctgttcggcg agtctctgct gttctttctc ctgctgacgc tgtttttctg ccagtcgctc
     7561 catttcctga tgtatcccct gaaactccgc cattgtctcg ttcacaaggg actgaaggtc
     7621 gatttcgtcc tgtacgtcct tcagcgcatc actgaccagt gcgttcagct tctccggctc
     7681 tttctcaaaa tcgaaaaccc tgccggaagt gtcttcctgc gcaggttcag gcttctgctc
     7741 ttgttttatc gtcaggttat cccgctcgct gatggcttcc cgtagcgagg tggtcacgtc
     7801 aatcacactg tcacgttcat cacgagactg ctccgcctgc ctttcagcgt ccctgcgctc
     7861 aaggatggcc tgtaactgcg cagtatcgaa ccgctgaacc tgtcccgcgc caagatgccg
     7921 ctcaggctca cgctcaatgc cctgagcttt gagtgagcgt gcatcaacgc ggtcggtatg
     7981 ctgataccgt tcaaggtgct tattctgtag gtcagcccag cgttcccgct gggcgacgag
     8041 gtgctctttg cgttctgtcg gcgtttcccc gaaacgtgcc tttttcgcac cgccgcgctc
     8101 aggggctttg ctgttagctc gcttaaaata ctgctgcggg tcacggtcga taccgtcatt
     8161 catccgctca gagaacatga tatgcgcgtg cggctgctcg ccgccggcta tcgccgcttt
     8221 cggattatga atggcgaact gatacgcatg acggtcgccg atttcctgcc ggacaaaatc
     8281 gcggaccagt tcaaggcgtt gttcgggttt cagctcacgc ggcagggcaa tctcgatttc
     8341 acggtaggta cagccgttgg cacgttcaaa cgtgtcagcg gctttccaga actccgtcgg
     8401 tttatgcgct gcccaggcag gcatattgcc ggactccttg tgctcaaggt cggagtcttt
     8461 ttcacgggca tactttccct cacgcgaaat ataatcggca tggggagagg cactgccttt
     8521 cccgccggtt tttacgctga ggtgatagga ggccatcgtg tttttatccc gctgaaggcg
     8581 cgcaccgttt ctgaacgaag tgaagaaacg tctaagtgcg cactgataat aaaaagctta
     8641 tcagtgattc tacctgcatt tgacctcggc cgccattcct gaccataatt tccacatcaa
     8701 cattcagagg ataaatgggc atgaataaaa gcgaaaaaca ggaaaaatgg gcagcagaca
     8761 gagtgcagta cattcgcggc ttaaagtcgc cgaatgaaca gcagaaactc atgctgatac
     8821 tgacggataa agcggataaa acagcgcagg atatcaaaac gctgtccttg ctgatgaagg
     8881 ctgaacaggc agcagaaaaa gcgcaggaag ccagagcaaa agtcatgaat ctgatacagg
     8941 ccgaaaaacg ggcagaagcc agagccgccc gtaaagcccg tgaccatgcc ctgtaccagt
     9001 cagccggact gcttatcctg gcgggtctgg ttgacagcca gacggggaag cctgttgatg
     9061 ataccgctgc cttgctgggt gcattagcca gtctgaatga cctgtcgagg gataatccga
     9121 agtggtcaga ctggaaaatc agaggtcagg aactgctgaa cagcaagaag tctgacagct
     9181 ctgcatagca gacctgccat aaaacgccct gagaagcccg tgacgggctt ttcttgtgtc
     9241 atgggtagat ttcctcgtgt gagtccatga aaagcgcctg tagcatcatt tacccccatt
     9301 ccctgccaga gccgtgagcg tagcgaactg aatgtcacga aaaagacagc gactcaggtg
     9361 tctgatggtc ggagacgaaa ggaatattca gcgatttgcc cgagcttgcg agggtgctac
     9421 ttaagccttt agggttttaa ggtctgtttt gtagaggagc aaacagcgtt tgcgacatcc
     9481 ttttgtaata ctgcggaact gactaaagta gtgagttata cacagggctg ggatctattc
     9541 tttttatctt tttttattct ttctttattc tataaattat aaccacttga atataaacaa
     9601 aaaaaacaca caaaggtcta gcggaattta cagagggtct agcagaattt acaagttttc
     9661 cagcaaaggt ctagcagaat ttacagatac ccacaactca aaggaaaagg actagtaatt
     9721 atcattgact agcccatctc aattggtata gtgattaaaa tcacctagac caattgagat
     9781 gt
//
