LOCUS       NZ_CP040836            10060 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain SH12 plasmid pSH12_3, complete
            sequence.
ACCESSION   NZ_CP040836
VERSION     NZ_CP040836.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN11963759
            Assembly: GCF_019444635.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Yang,X.
  TITLE     complete sequence of Klebsiella pneumoniae strain SH12
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Yang,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JUN-2019) The Department of Applied Biology and
            Chemical Technology, The Hong Kong Polytechnic University, Hung Hom,
            Kowloon, Hong Kong 999999, Hong Kong
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina NextSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 03:49:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,944
            CDSs (total)                      :: 5,823
            Genes (coding)                    :: 5,667
            CDSs (with protein)               :: 5,667
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 67 of 156
            Pseudo Genes (incomplete)         :: 96 of 156
            Pseudo Genes (internal stop)      :: 20 of 156
            Pseudo Genes (multiple problems)  :: 26 of 156
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP040836.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SH12"
                     /isolation_source="sputum"
                     /db_xref="taxon:573"
                     /plasmid="pSH12_3"
                     /geo_loc_name="China"
                     /collection_date="2016"
     gene            join(10056..10060,1..1681)
                     /locus_tag="FHY43_RS29355"
                     /old_locus_tag="FHY43_29950"
     CDS             join(10056..10060,1..1681)
                     /locus_tag="FHY43_RS29355"
                     /old_locus_tag="FHY43_29950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            1691..1948
                     /locus_tag="FHY43_RS29360"
                     /old_locus_tag="FHY43_29955"
     CDS             1691..1948
                     /locus_tag="FHY43_RS29360"
                     /old_locus_tag="FHY43_29955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            2033..2182
                     /locus_tag="FHY43_RS29365"
                     /old_locus_tag="FHY43_29960"
     CDS             2033..2182
                     /locus_tag="FHY43_RS29365"
                     /old_locus_tag="FHY43_29960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            3666..5630
                     /locus_tag="FHY43_RS29370"
                     /old_locus_tag="FHY43_29970"
     CDS             3666..5630
                     /locus_tag="FHY43_RS29370"
                     /old_locus_tag="FHY43_29970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            5630..6361
                     /gene="mobC"
                     /locus_tag="FHY43_RS29375"
                     /old_locus_tag="FHY43_29975"
     CDS             5630..6361
                     /gene="mobC"
                     /locus_tag="FHY43_RS29375"
                     /old_locus_tag="FHY43_29975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            6368..6898
                     /locus_tag="FHY43_RS29380"
                     /old_locus_tag="FHY43_29980"
     CDS             6368..6898
                     /locus_tag="FHY43_RS29380"
                     /old_locus_tag="FHY43_29980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(6925..7104)
                     /locus_tag="FHY43_RS29385"
                     /old_locus_tag="FHY43_29985"
     CDS             complement(6925..7104)
                     /locus_tag="FHY43_RS29385"
                     /old_locus_tag="FHY43_29985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(7130..7558)
                     /locus_tag="FHY43_RS29390"
                     /old_locus_tag="FHY43_29990"
     CDS             complement(7130..7558)
                     /locus_tag="FHY43_RS29390"
                     /old_locus_tag="FHY43_29990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(7722..8036)
                     /locus_tag="FHY43_RS29395"
                     /old_locus_tag="FHY43_29995"
     CDS             complement(7722..8036)
                     /locus_tag="FHY43_RS29395"
                     /old_locus_tag="FHY43_29995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(8024..8302)
                     /locus_tag="FHY43_RS29400"
                     /old_locus_tag="FHY43_30000"
     CDS             complement(8024..8302)
                     /locus_tag="FHY43_RS29400"
                     /old_locus_tag="FHY43_30000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(8477..8842)
                     /locus_tag="FHY43_RS29785"
                     /old_locus_tag="FHY43_30005"
     CDS             complement(8477..8842)
                     /locus_tag="FHY43_RS29785"
                     /old_locus_tag="FHY43_30005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(8839..9210)
                     /gene="tolA"
                     /locus_tag="FHY43_RS29410"
                     /old_locus_tag="FHY43_30010"
     CDS             complement(8839..9210)
                     /gene="tolA"
                     /locus_tag="FHY43_RS29410"
                     /old_locus_tag="FHY43_30010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(9484..9729)
                     /locus_tag="FHY43_RS29415"
                     /old_locus_tag="FHY43_30015"
     CDS             complement(9484..9729)
                     /locus_tag="FHY43_RS29415"
                     /old_locus_tag="FHY43_30015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     BGC             1..6681
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(2286..2864)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5631..6358
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 tggtggagac ggtcgaggtc cgggtaattc aggtctggga cataatggtg gtcaggccag
       61 tgggaatgtg aacggtacgt ctggtaaagg tggcccttca tcaggtggtg gtacggatcc
      121 aaacagcggg ccgggctggg gtacgacgca tacgccgaat ggagatattc ataactataa
      181 tccgggggag tttggtaacg gcgggagtaa acccggtggt aatggtggta acagcggcaa
      241 tcatagcggt agctctggtg gcggacagtc ttcggccact gcgatggcct tcggtctgcc
      301 tgccctggct actccggggg ctgaaggact ggctttatca gtttccggcg atgcgttgtc
      361 ggccgccgtt gctgatgtgc tggctgccct gaaggggccg tttaagtttg gtctgtgggg
      421 gattgcgatc tacggtgtgc tgccttctga gattgcaaaa gatgatccga aaatgatgtc
      481 aaaaattatg acgtcattac cggccgatgc ggtgacggag actccggcaa gtactttacc
      541 actggaccag gcgacggttc gtgtcagaca acgggttgtg gatgtggtga aggatgagcg
      601 gcagcatatt gcggttgtcg caggtcggcc aatgagtgtc cctgtggtgg atgcgaaacc
      661 gacaaaacgt ccgggggtat tcagtgtgtc gattccgggt ctcccgtctc tgcaggtgag
      721 cgtaccgaaa ggtgttccga cagcgaaagc cccgccaaaa ggcattgttg ctgaaaaagg
      781 tgattcacgt ccggctggtt ttacagccgg tggtaactcc cgtgaggccg ttattcgttt
      841 cccgaaagag accggacaga agccggttta tgtgtcggtg acagatgttc ttaccccggc
      901 acaggtaaaa cagcgtcagg aggaagaaaa gcgtcgccag caggcatggg acgccgctca
      961 tccggaagag gggctgaaaa gagactatga taaagcgaaa gctgagctgg atgccgaaga
     1021 taaaaatatt gcgaccttaa acagccgcat tgcatcgaca gagaaggcgc tccccggtgc
     1081 aagggctgct gttcaggaag ccgataaaaa ggtaaaagag gcagaggcaa ataaagatga
     1141 ttttgtgact tataacccgc ctcatgaata tggctccggg tggcaggatc aggttcgcta
     1201 tcttgataag gatattcaga atcagaatga gaaattaaaa gcggctcagg catctttaaa
     1261 cgcaatgaat gaatccttat ccagagataa ggctgcactt accggggcga tggagagccg
     1321 gaaacaaaag gagaaaaaag cgaaggatgc agaaaataag ttaaatgagg aaaagaacaa
     1381 gcctcgcaag gggactaaag actacggcca tgattatcat ccagtaccta aaacagaaga
     1441 cataaaggga cttggagagt taaagcgagg tgatcctaaa acacctaaac aaggtggtgg
     1501 tggtaagcgg gcccgatggt atggcgataa aaaacgtaaa atttatgaat gggattccca
     1561 gcacggtgag cttgaagggt atcgtgccag tgatggcgaa cacctaggcg catttgatcc
     1621 caaaacgggt aagcaggtta aagggccaga tccaaaacgg aacattaaaa aatatctttg
     1681 agaggtaaat atggggctta aattacgttt gacatggttt gataagaaaa ctgaagagtt
     1741 taaaggggaa gagtattcta aagactttgg tgacgatggt tctgtcattg aaagcttggg
     1801 aatgccttta aaggataata ttaataatgg tgagtttgat ttgaagaaag aatgggttcc
     1861 tttactacag ccatttttta aaaatcaaat aaaacctgat gtggataatt gttatatagc
     1921 ttttgattat cgggacggta actggtaaca aaaaattatc aaggatgatt tgaaatactg
     1981 acttccggtg ttcagggatg aatgccggag tctttaaata cagggaaagt atatgaaaaa
     2041 ggtaaagact atttttcttt ttatattgat tgttgctggc tttcttctgg ccgcatgtca
     2101 ggcaaattat atccgggatg ttcagggcgg aacggtggca ccatcgtcct cctctgaact
     2161 gacggggatc gcggttcagt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     2221 cgcgtaatct tttgccctgt aaacgaaaaa accacctggg gaggtggttt gatcgaaggt
     2281 taagtcagtt ggggaactgc ttaaccgtgg taactggctt tcgcagagca cagcaaccaa
     2341 atctgtcctt ccagtgtagc cggactttgg cgcacacttc aagagcaacc gcgtgtttag
     2401 ctaaacaaat cctctgcgaa ctcccagtta ccaatggctg ctgccagtgg cgttttgcgt
     2461 gctttcccgg gttggactca agtgaacagt taccggataa ggcgcagcag tcgggctgaa
     2521 cggggagttc ttgcttacag cccagcttgg agcgaacgac ctacaccgag ccgagatacc
     2581 agtgtgtgag ctatgagaaa gcgccacact tcccgcaagg gagaacggcg gacaggtatc
     2641 cggtaaacgg cagggtcgga acaggagagc gcaagaggga gcgacctacc ggaaacggtg
     2701 gggatcttta agtcctgtcg ggtttcgccc gtactgtcag attcatggtt gagcctcacg
     2761 gctcccacag atgcaccgaa aaagcgtctg tttatgtgaa ctctggcagg agggcggagc
     2821 ctatggaaaa acgccaccgg cgcggccctg ccgttttgcc tcacatgtta gtcccctgct
     2881 tatccacgga atctgtgggt aactttgtat gtgtccgcag cgcccgccgc agtctcacgc
     2941 ccggagcgta gcgaccgagt gagcgaggag gcgcaatttc cccggcacct gcaagacggt
     3001 caccggacgc gctgatgtgg gatattgtcc cctgattaag tctttctatt ccctgacgat
     3061 caattatcag gataggtgaa ccatgtcggt ttttcgctga acattttacg ttaacctatc
     3121 tctgttgctt ttttctcctt gcggagaaat gcaagtcatc cccgctgggg cgtggctgac
     3181 ttcaggggaa aagaccttgc ttacgctggc tggacgccac cgacgcacgg tcttttcccc
     3241 tctttctggc tctgtccggc tggacgccgg ccacgccaga aattcacccg gcccccgtca
     3301 ggctcacgcc tgaccaccca caaaaatcca cggtgctgga cgcacctgac gaagcagcaa
     3361 agctgctcct gattttgcgg gtggtcagcc gctcgctatg gcgaactctg acgggggccg
     3421 gaacaccgga aaagaccgca ctggggtgcc gccttttcct ctcacacgct ggggcgtgct
     3481 cgttgttcct ccggggctgc gccttttccg catggggcgg aaagaggctg gcctccgcgg
     3541 tgaaagctga acgcaatcac cacggacgtg accgccgccg ccatgccttg gggcatgtcc
     3601 gcggcttgtg cagcgggggc tgcgcatcac gccggtaaac ccgtcgtctg aaggagagag
     3661 attgcatgtt taatacggat tcgcttgcct ggcagtggca tatcccggag ctggcccggc
     3721 agatttaccc gtgggtcagc tggctgtacc gggagtatta cagtctggcc ggtttttttc
     3781 tggttatcgg cctgtttttc ggtctggctg aactctgggc cggccgaatg cgttttgtgg
     3841 ggctgagcca taaggcgcgg cgggagtatg ccgatcgcta taagcgcgat aataaccgga
     3901 ctgtgccatt ttcggtgctg gcctcgctgg attcacccgg gcggatggtc agggcacggc
     3961 gacatgtctg gaactacgcg tactggctga ccggcgctgc cggtctggcc cttctggggg
     4021 ggctggttgt cgccgtgttt atcgagagct ggggagaaac cgtcctgcgc ccggacctct
     4081 atgcgaacgc agattattac ctgctgcagc cggctaacct gttcgggata gcctggtgtg
     4141 ccggagggct tgtcgccggc gtccttctgg cgcggctgct ggcgttcctg ctgcttgatg
     4201 gtcattttgc ggccgctgac gaggcggtga acgcgaaact gaatcaggag agccggcgca
     4261 gcagccagcg gaccggagaa atgacagacg tccgccacct gcatttcggg gagccggtac
     4321 cggttaacgc gctggccgat ttttccacgg agcaggcccg gaaacagcag gcggtgtttc
     4381 tgggtaagga tgagcagggc cagccggtgc tggtccctcg tgatacctgg cgcaagacca
     4441 atattcagat cctcggcctg cccgggagcg gcaagagcgc gatggccacc aacgcgctca
     4501 ttcgctgcgt acgcgatttc ggggacgctg tggtgtactt cgaccccaaa ggggacgcgt
     4561 gggcgccgca cgtcttccgg gcgcactgcc cggacttcac cctgctggat ctgcggccgg
     4621 ggaaaccggc gcagctgaat ctgttccgcg atctcgacca gtacgccctg aagaatctgc
     4681 tggttgccgg gtttaacctg agtgagaccg gcgacgtggc ggaccactac cgcatcagcg
     4741 agcagaaagc ggcgaagctg attgcggagc agttcccgca gggggcaaac attcagcagg
     4801 tgctggcggc ggcgtacgcg ttgccggagg ccctgaaaaa ggacgtgaag gggctgatca
     4861 ccaagctgga gaacgtggcc gacctgagcg tcctgcagac ggacagcggt atcgatgtgg
     4921 cggggataat caacggcggc ggctgcctgt acgtcatcgg ctcgatggac gatgaggcgg
     4981 tgatccgggt gcagaagatg ctgtttgccc gctgcgcgca aatcatcatt gcgcgggatg
     5041 agttccggag gtggccgcac gcgagcatca tgctcgatga gattaaatat ctgctgtcga
     5101 agtacgtgct gaacgcgctg gggacgctgc gcagccggga ctgcaacctg ctgctcgcgc
     5161 accagtcgct gggcgacttc ggtcagtgcg ggcaggatct gcccgctgat tttgtgaaaa
     5221 ccacggtact ggataacacg ccgatccgct ggttctatcg cgcggccagc caggagtccg
     5281 cgcagtgggc ggcgggccag accggggaga tccgggtgga cgtggagcgc cgccgggcca
     5341 gccgggaggc ggggaacgtg gagcacatca gcggcgacag cttcatccag aaggaggcgc
     5401 ggccgctgtt tgacgtgaac accctgcaac acctgcctga cgggttcgcg gtgatgaccg
     5461 ggctgggcgt ggcgcggctg gcgttcagca gcccgctgcg ggttgagcgc cgggaaattt
     5521 cgctgaaaac cttcccggtg ctggcgaaaa cggacccgct ggcggagtac ctgcctccgg
     5581 ccgacggcgg cggaccaaaa gcggatgatg attttgcggg gctttactga tgacactgga
     5641 gcagtacaac gtcagccacg cgaagcggca ggcgcggaac gcggagaaaa cacggctgac
     5701 cctgcgctgg ctgcgggaag agctgtgttc cacggcggag ctggttgcgc gacggctggg
     5761 gattgcggcg gtacagccgg tttaccgctt cctggacagt ctggtggcga aagggctgct
     5821 ggtccgggcg aaatacccgg ttgacggccg tcaggtcagc gtgtgggggc tgacgccgca
     5881 cggggtggcg ttctcgttcg acgaggacga gccgctgacg gacgttattc cgtttcagcc
     5941 gtcaagggtg tcggcggcgc agctgccgca ccggctggcc gtgcagtccc tgcgcctctc
     6001 catggagggc cggggggcca ccggctggcg gtacctgcac cgtatggccc tgaaagggat
     6061 gaaagtgccg gacgcgctgg cggagctgga cggcagaacg gtggcatttg aggtagagcg
     6121 caccgtcaag agccgcaggc ggtatcagga agtggtgtcc gggtatctgt tcaaccgccg
     6181 ggcgaacggc atcgatgaaa tctggtacat ctgccctgac cggccgacgc ttgtccgggt
     6241 acagcgggcg attctggcgg tggatgaaat cgtgaatcca cagaccggcg aggcccgcaa
     6301 aacggtggag ctggaccggg agaggctgtt cgcgtgcttt aaatttatga caacggagtg
     6361 agggattatg gcgctgtaca tggtatttat tgaggacgtg gaagacaaag acggcgagcg
     6421 ctgcattatg ccaaccagcg agcatctgga agacatggtg gtgctcgcct ggcagaccgg
     6481 ggcgtatgtt ttctgttttg tcgaccgggc ccagcatctg gcgatgatga aatccatcgg
     6541 gatgtatgac gagcggctca acagcgacga aaaagccacg tactttttca ccggtgaaaa
     6601 tccgcaatgg gaacgggaca aacccacagg aatgaagctg tgtgattacc tctatcgaaa
     6661 ggtggagcgg ttcgccatcg agaacggtgt gcaggtggtt gatgccggga agtgctcacc
     6721 ggagcagatt aacgcgatga ttgaggtggt cagcgagcgg cacggggaaa gcgcggtgaa
     6781 cgagaagctg aaagcggagc tgatgcggga atataacagc cgctatttta acgtgccggt
     6841 gcctgaactg ccggaggaca aacccgcccg ccggtggtgg ccgttcggca aaaaataacg
     6901 ccagcaccgg catggccggt gctgttattc ttccccgagc ttatcccgca tcacctgcca
     6961 gagtgtttcc gcatcctcgt gaagccgttc gcacatgtcg gccggggtat ccatgtccag
     7021 ctcgttcagc ttttccagca acagcagcga ctgatcctta atgaatttag ccatattgag
     7081 ggcagttttt tcctgcttcg tcatgttctc cattcccttc ggtaagttct cagctaaacc
     7141 agccggacca gaagcgtttt ttcttctgtg tcagttctgt tgtcaatctg tccacttcgg
     7201 cctggagttt ccggcgctca tcatcgtgct ggcggcgttc ttcttcccgg gatgttttat
     7261 cttccagcat cagggtgaca gcctgccgga gatcggccag ttccttttgc attgccagaa
     7321 gctgttcctg tgggaaattt ttttgtgtat tttcacggtg tgaaatgccg ggaatttgtt
     7381 tctcagggat attcacattt ccgtatacac ggatcagttc tgacacatcc acgacagggt
     7441 tgtttttccc gtcacgtgac acggtaacct ttccctgttt tacatgattg tatagcgttc
     7501 ttctggtaat gcccgcggct tgtgctgcct gtgaaaggtt gagcaatgtt ttcgccactg
     7561 tatacccctg attttatcgg gtgtgaaagg tgtgtaattt ctcacggtaa atgtatcgct
     7621 gaattttacc cgtttttccg ttcaggatgt ggttttctga cgcgctgcgc ttgtcaaacc
     7681 ggcgacggcc acgcaatgaa ttgcgccccc gtcatcggtt attagaggct gtctgccccc
     7741 ggatacccgg attctttctg atgcctgacg gtgacgatca caatctggtc catttcccgg
     7801 tcgtggcggt agagcatcac atagccactg tcgccaaacc cgatcaccag ttccttatat
     7861 tccagcggca gaaacggcac cgggcggcca atgtctggca atgttttcag ttgctggata
     7921 gcccggacga tcacctcacc tccttttctg gctgccagcc gatttttggt tttgagaaag
     7981 tcctgaaggc gttgtaaatc gcgctgtgcc agcgcggaaa tcgttacctg tggcatggcg
     8041 gagcatcctg ctcgttctca gttccccagg tacttatcca ggcttccgct tcttccgcag
     8101 taaggtgcag acccgtttcc tgatagtgct gccatgcggc ctgtccgtcc cgcaggtact
     8161 ggctgcgctt ctcttcccgg tcgatgtatt cggtgattgc ctccagcatt agcgcgtgcg
     8221 ctgagcggtg ccggtcatcg gccagcgttt tcagccggtc cttaagttct tcatcgagcc
     8281 tgatggatgt tgctgatgac ataaaagccc ctttgtagtc atatgtatta cacatgacta
     8341 ctttaatccg tggcggcgct atttgccaga aatacggctt ttgctgccca tttttcagct
     8401 tcgcttccac taatcagggc tgctacaggc tcactcatca tccaggacta ttgcagcctg
     8461 cgggttaaag gacctgttac tggcggcaac catcatcatt gagcttttgg gtggtgactg
     8521 gtcgcggatc ctgtaagccg tcatttttca gactgaattc aaaatttata acctgtcgca
     8581 ttgccgggtg ccctgcggga tatagccagt gcctcattgc cgtcagcgtt tcccgaaaaa
     8641 aaaggtgaca gccggtaagc gacacgttgt ccactcgtcc atcgtccaga atatcaaacg
     8701 tggcggtcac ctgaccttcg atgcgttttg ctgcggcctg taccgggtag gttggctgca
     8761 gtgtggttac gggttttagt ggtatcgcat gtaccggagt gaggaacatg gccgccagca
     8821 ctgtcagagt aaaagctttc atggtctgaa atccagaggg gcgtttttga atatttgcca
     8881 ggtattttca tccggtgccg gcggaatttt ggcgtgggta acggctgata ttgctgcttt
     8941 acagagtttc ggatctcctc cttcagcaat ggcgctgttc agagtgccat cacgttgtat
     9001 ggatatccgc acggtgcaca tttttcccgc ataggcctct ggttcaaaca tctgcatttc
     9061 aacagcggtc ttgatggccg cagcgtagct actgatttta gatgacttat ccagagtata
     9121 aattttcggc ggctgggtgg tggaatgctt acaggccgtc aggagtaagg ggctgcacaa
     9181 tgccagcagg ataaagggta ttaaacgcat cgggttgatg acctgtcagt aaaaaatgtt
     9241 acatgtatat ttgtcactct ttacggccac gttcatttac ataaacacgg cttatcgtat
     9301 cgtttttaca ccaaacggcc cgccatgcag ttgcgtgcat aggcatgcag cgggttacaa
     9361 tttaccgggc gcgtttccgg cggttttccg ggtggtttgt tgcctgtttt atcccgtagc
     9421 cgccggaaac gccctcagac cgtctgagcg gtgcgggcaa tgcgacgtta tggtaaatac
     9481 cccttaccgg cggcgctgtg gctgccaggt gaaggtggtt tccgtagcct cgtatttctc
     9541 ccgacgcatt tcctgcgccc gcctggcttc acgttcgtcc tgaatggcac gaagcaccct
     9601 ttcccgcacc tgttgtttta ccgcctttgg cagcgttttg ttccctttca ggcccatggc
     9661 cagtgcctga gcgacaaagg cgtccgccag gcgcttttct tcagcgggag tcagtttcag
     9721 gtgcgtcata gtggcctccg ggcgtctcag gtttcccgaa cagtttaacc gcaggtcctc
     9781 tcctcatgcc agttagcttt cagatgtgtc tataccatct gaaatgtaag tgtgatgaat
     9841 gtcttaaata ataaaatttg ttgccatgag aaattttcgg gaaaagacgc gatctgctta
     9901 caggtggctc cgggcgcttt tttgtgcctg aagatgagaa ctaatctcat atattgacac
     9961 ctgaaaacgg gaggagtaag gtaataatta tactgtgtat atgtacagta tgttggcttt
    10021 aatttattgt tttaaatgtc aaagaggaaa acggtatgag
//
