LOCUS       NZ_CP040727             3843 bp    DNA     circular CON 08-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KpvST147B_SE1_1_NDM plasmid
            pKpvST147B_4, complete sequence.
ACCESSION   NZ_CP040727
VERSION     NZ_CP040727.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN11835544
            Assembly: GCF_005944305.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Turton,J.F., Davies,F., Turton,J.A., Perry,C.L., Payne,Z. and
            Pike,R.
  TITLE     Hybrid resistance and virulence plasmids in high-risk clones of
            Klebsiella pneumoniae, including those carrying blaNDM-5
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2019) Healthcare Associated Infection &
            Antimicrobial Resistance Division, Public Health England, 61
            Colindale Avenue, London NW9 5EQ, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 21-MAR-2019
            Assembly Method :: miniasm v. 0.2-r168-dirty; racon correction (3
            iterations) v. 1.3.1; racon correction with Illumina reads v. 1.3.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 243.0x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/08/2024 05:30:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,078
            CDSs (total)                      :: 5,954
            Genes (coding)                    :: 5,634
            CDSs (with protein)               :: 5,634
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 220 of 320
            Pseudo Genes (incomplete)         :: 118 of 320
            Pseudo Genes (internal stop)      :: 40 of 320
            Pseudo Genes (multiple problems)  :: 52 of 320
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP040727.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KpvST147B_SE1_1_NDM"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKpvST147B_4"
                     /geo_loc_name="United Kingdom"
                     /lat_lon="51.38 N 0.18 W"
                     /collection_date="2019-01-14"
                     /collected_by="Hospital in South East England"
                     /genotype="ST147"
     gene            179..370
                     /locus_tag="FGF03_RS28955"
                     /old_locus_tag="FGF03_28955"
     CDS             179..370
                     /locus_tag="FGF03_RS28955"
                     /old_locus_tag="FGF03_28955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            370..771
                     /locus_tag="FGF03_RS28960"
                     /old_locus_tag="FGF03_28960"
     CDS             370..771
                     /locus_tag="FGF03_RS28960"
                     /old_locus_tag="FGF03_28960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1117..1359)
                     /locus_tag="FGF03_RS31280"
     CDS             complement(1117..1359)
                     /locus_tag="FGF03_RS31280"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098963735.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(1725..2357)
                     /locus_tag="FGF03_RS28970"
                     /old_locus_tag="FGF03_28970"
     CDS             complement(1725..2357)
                     /locus_tag="FGF03_RS28970"
                     /old_locus_tag="FGF03_28970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="WP_012579086.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="DUF3226 domain-containing protein"
     gene            complement(2357..3472)
                     /locus_tag="FGF03_RS28975"
                     /old_locus_tag="FGF03_28975"
     CDS             complement(2357..3472)
                     /locus_tag="FGF03_RS28975"
                     /old_locus_tag="FGF03_28975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_012579087.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="ATP-binding protein"
ORIGIN
        1 acggccgctg tgcggccttt ccttctttcc tccatgcgat tactcggttt tctcgagcgt
       61 ttctacgcat gctcctgccg ctcgccgcag ccgaacggca ggagcgtagc gactgagtga
      121 gcgaggaagc ggaaaagagg agatccctac actgaccact gacgctctga ggcctcttat
      181 gacgaaacaa cagaaaacag cgctgaacat ggctaaattc atccagaatc agtctctgct
      241 cctgctggag aagcttaacg agcttgatct cgatgtcgag gccgaccttt gtgagaaact
      301 ccacgatgat gcggagcatc ttttccgtac actgtcctcg agactggatg cattacagga
      361 tggtaactga tggccagagt gtctattagt gaggctgccc gactgacggg taagagtaga
      421 acaacactgc acaggttaat taaagcaggt gacctgtcca cctgtagcgg tgagcgcaat
      481 gcaaaaatgg tagatacttc agagctttta cgtgtctttg gcccctttga gcaaccgaag
      541 ggtgaacacc attctggaca ggtctctgga cagcgtgata cagcctctgc tgaccagagt
      601 gaacaggtga tccgtcatct gaaacaggag attgaacacc tgaacacctt acttttgtcc
      661 aaggattcgc acattgacag cctgaaacag gccatgctcc ttcttgaaaa tcaccaggca
      721 aaattaactg aatccccggc gccatggtgg aaattctgga aaaaatcctg accccgtcat
      781 gcttcagttt tcctctccgg ccatcccagg gagttcctga accttcccaa aaatcgtcag
      841 atctctgaac gctcctcacc actaaaaata cccacacccc acgcaaaatt aaaattttgc
      901 agattcctta tttacaacaa tgggttaatg aaaaataaat gtgcggtgat gtgttacaaa
      961 gtgcagatcc aagtcgtgga cgactctatg gcgaatcgct gacgaattgc ccacgattca
     1021 tatgtgaatc acgctttatt atcatattgt tatgatgaat cgactcttga ttcaattcat
     1081 cactttgggt gttttttgct catggtgact cttgattcaa ttcacgattc gaatcgccgg
     1141 tgaatcgtgt ggtgagtcgt ccggcgactt gccataacgt gattgccggc cgtcgacgaa
     1201 cccgattcct gaaggtgctg aaaaccggtt ttccggctgc gccggcaacg cttcacggcc
     1261 acgcaataaa ttgcgtcccc gttcaccgtt ttcggaggag aagaacgccg gaaacaggac
     1321 ttcccctggc tgaatggtgt gaatttccgc gctatgcact tgcgcgcata ctcatgcatg
     1381 ccgtaaaaac agagcctgcg cgtttctggc gggttttcgg gtggtttgtt gcctgtttta
     1441 ccggtttccc gtcagaaacg tcctgagtcc gttttagcgg tgcgtacaat tatgatttta
     1501 tgttaaattg ttttaagtga atagagagaa tataacttaa gggagcatct tgtgctgggt
     1561 cttttacatt agatctgcac aatcattgtt ttggcatctg gtgatgtctt taggacgttt
     1621 gagattgctg ataactaaag tgtatgatgc tttctatacg ttatccggcg tttaataaca
     1681 ccctggtgat aagccagccc gtgaagggct ggcttgattt ggatttatat ttttgaaata
     1741 aaatctttta tttcatcaaa gcaattgtct gctgtgttta ttattccatt tttcatggat
     1801 attcctattc ttgagtcgga ttctctattg cagctaagat agacttgtac cttagctttt
     1861 ttttcgtttg ttggataagt gtactctttg tcactcgctg agatgtcatc tttattattt
     1921 ttaatttttt cgaaaaattc tattatgtat tcatataacg ctcgagtgct tgcgtgttca
     1981 attatcagat cttcaatggc accttgcttg tcttttcctg gcattataaa gaaagaaatt
     2041 tttgtattgc cattcatagc tatctcgtta tgcgagctgg gggaggagat ttgtagttct
     2101 cgcaatgtgg caattattga tctttctgtg tcagaaaatg atgtgtcagc atctaatata
     2161 ataaaaatac tttctttttc atcgaaagaa tcaatgtttt tgaatgctct aagtgcttgt
     2221 tttaattttg attttccatt tattttttcg acaaaaacat cggtaatgcc ttgtatttca
     2281 agtaggttgc atagaaaatg aaaatcatca tcaccctcaa cgaaaatgat ctttttgcta
     2341 ttatttatgt ctttcattat cttacctccc agtcgttctc aactgagtag gagaaatcat
     2401 ccatattgaa ttgtacggct gttggtttta gggagttctt ttctcttcct agtctaatat
     2461 aagcgatatc ttcatttttg ctatctattt cgtttattgc tcgtatcaca tcttgactat
     2521 gagttgttgc aaatatttga ttgttgtttt gttttgcaga tgaaattaat gattttatta
     2581 tgtctttaat tactgagtaa tgtattccgt tctcgatttc atcaataaga ataattgagt
     2641 tttcctgtac cagcactgag ctgataaaag ataatgctcg actgataccc tcgccaagca
     2701 ttgatatttc atttagctca ctaaatccaa tgtctaaata tatttcctta ttgttaccta
     2761 tcgcagatat tgctatatct acaattctgt catctatttt ttttaagttt tctagtatgt
     2821 cttttttcct tttttgtgtt agtaggctag atacatttgt aattgttgcc tctttgttta
     2881 tctttctgga tgttgttatt attgttgctg tcttaaattt ataattaatc ccatcccttt
     2941 cgttttttat ttcggatttt atttctacgc catcttcttt caattgcata gagaaaagct
     3001 tattggaaac cctgtttttc gcattgggtg tctcggtcac atttattttt aaagagttta
     3061 ctgtggcctg agaaccgata gtttgacgag gtatgttttg gttagatagt acggtgtcaa
     3121 cgtttagaga caaacttgat tctaatctct gatttgtttc tgtttcatat gtttgtgtta
     3181 cttcagagcg ttcatctcga atgactatgg acatcttttg tgatgtgtcc atatcatgaa
     3241 agtaactaac ccaaaatggc ttgtttttgt ttatttctgc catttctttt ctgaatgctt
     3301 gaatgtttaa caaaacagcc gggtttttta catcataaaa acaaaaaata gcatccagta
     3361 tagatgtttt cccgtagttg tttttcccag ctatgatatt taaagtcttt aattgcttta
     3421 gatatagctc attgaagcat ttgaagttct ttaaacttat ttcctttatc atgatagcct
     3481 ctgttcgtgt ttttgttata tgccatacga tttgttagta ccttaataaa atgtacagct
     3541 ttcattgtga ttagctagac tttatgtcaa agattaactt tgattgcttg tgttttaatc
     3601 gatttttttt actttcttct tgagattatc tggcagcagc cgtaagcttc cggctgtgaa
     3661 cgaaaaaaac cgccttggcg ggcggttttt tcgaaaggtt cagtagagtt ggcgctctat
     3721 gaaccgaggt aactggctta aagggagcgc agtcaccaaa actgttcgtt tagtttagct
     3781 ttaactggcg tataacttca agactaactc cctaaatcag ttaccagtgg ctgctgccag
     3841 tgg
//
