LOCUS       NZ_CP040716             5596 bp    DNA     circular CON 25-JUN-2023
DEFINITION  Klebsiella pneumoniae strain 16HN-35 plasmid p16HN-35_5, complete
            sequence.
ACCESSION   NZ_CP040716
VERSION     NZ_CP040716.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN11867826
            Assembly: GCF_030323825.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Yang,X.
  TITLE     complete sequence of Klebsiella pneumoniae strain 16HN-35
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Yang,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2019) The Department of Applied Biology and
            Chemical Technology, The Hong Kong Polytechnic University, Hung Hom,
            Kowloon, Hong Kong 999999, Hong Kong
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/23/2023 17:46:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,828
            CDSs (total)                      :: 5,708
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 252
            CDSs (without protein)            :: 252
            Pseudo Genes (ambiguous residues) :: 0 of 252
            Pseudo Genes (frameshifted)       :: 159 of 252
            Pseudo Genes (incomplete)         :: 118 of 252
            Pseudo Genes (internal stop)      :: 30 of 252
            Pseudo Genes (multiple problems)  :: 54 of 252
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP040716.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="16HN-35"
                     /isolation_source="sputum"
                     /db_xref="taxon:573"
                     /plasmid="p16HN-35_5"
                     /geo_loc_name="China"
                     /collection_date="2016"
     gene            join(5219..5596,1..132)
                     /locus_tag="FGL96_RS29095"
     CDS             join(5219..5596,1..132)
                     /locus_tag="FGL96_RS29095"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            139..351
                     /locus_tag="FGL96_RS29100"
                     /old_locus_tag="FGL96_29485"
     CDS             139..351
                     /locus_tag="FGL96_RS29100"
                     /old_locus_tag="FGL96_29485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1018..1242
                     /locus_tag="FGL96_RS29105"
                     /old_locus_tag="FGL96_29490"
     CDS             1018..1242
                     /locus_tag="FGL96_RS29105"
                     /old_locus_tag="FGL96_29490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            1226..1492
                     /locus_tag="FGL96_RS29110"
                     /old_locus_tag="FGL96_29495"
     CDS             1226..1492
                     /locus_tag="FGL96_RS29110"
                     /old_locus_tag="FGL96_29495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1562..1912)
                     /locus_tag="FGL96_RS29115"
                     /old_locus_tag="FGL96_29500"
     CDS             complement(1562..1912)
                     /locus_tag="FGL96_RS29115"
                     /old_locus_tag="FGL96_29500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(1978..2523)
                     /locus_tag="FGL96_RS29120"
                     /old_locus_tag="FGL96_29505"
     CDS             complement(1978..2523)
                     /locus_tag="FGL96_RS29120"
                     /old_locus_tag="FGL96_29505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(2549..2875)
                     /locus_tag="FGL96_RS29125"
                     /old_locus_tag="FGL96_29510"
     CDS             complement(2549..2875)
                     /locus_tag="FGL96_RS29125"
                     /old_locus_tag="FGL96_29510"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            4031..4207
                     /locus_tag="FGL96_RS29130"
     CDS             4031..4207
                     /locus_tag="FGL96_RS29130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            4197..4541
                     /locus_tag="FGL96_RS29135"
                     /old_locus_tag="FGL96_29520"
     CDS             4197..4541
                     /locus_tag="FGL96_RS29135"
                     /old_locus_tag="FGL96_29520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(4723..4968)
                     /locus_tag="FGL96_RS29140"
     CDS             complement(4723..4968)
                     /locus_tag="FGL96_RS29140"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032433971.1"
                     /translation="MQPQSPVHHGLPRIDARTVCPVVVRLETPAGQQFRVRYQKIQFQP
                     PLVPVFNPQHAVLVFIESGHQNPLEARHQLFTLAGR"
                     /name="hypothetical protein"
     Typing          3866..3945
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(3283..3680)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          4..467
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="4.25e-73"
     Typing          4532..5595
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.718"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gtgacgtacc tggaagacag caacggacgt tttctggtgc tgccgaaggg gacggccgta
       61 gacaggacgc agagctggac ggtcgggaac gggcggtcga agcagaacgc gctgagactg
      121 gtgaaggagt aagtgccgat gaccgagctg gaaaaacagt tgctgagcgc attagagcag
      181 ctacagcagg actactcgaa aaggctggac gagtgggaga gcgccttcgg ggaatggcgg
      241 aaaatgtcag ggcttatgca acgggagaac gcggcgctga gcgagcgcgt gacgcgcttg
      301 agtcagcagg tggaacgctt gagcgggcaa ctgcagcgtt tgagccggta gtgcagcgtc
      361 atgagatggc cgtggccgca gaacgggcgc acgagcaacg gcagcacgaa aaggaactga
      421 cggaagcgcg ttcccggcag cgaagctatg acggtccgtc gctgggctga aatgcaggaa
      481 aacagggttc tcatcgggca tttacgctga aaccggatgt cattcacctg gctgcatggc
      541 tatgcagcca ggtaaaattt atctgtcgcg aacggggcgg tttatcgggt ggtttgttgc
      601 cggttttacc tgactgccac cccgttcgcg gcgaacccgt ccggggcggt gcgggcaacg
      661 ggtgttatgt ttaatcagcg tgaacggcag atacaaaaaa gccccggagg gcttttaact
      721 actgacttat ttttttgaag atttgctggg tttcaatttg tcagtatttc ccggcgtttt
      781 tttattgtca cgctggcgtt tatcaggttc ccctgttgaa tcggcaattc gtttcggacc
      841 cggcttaaga cccataatcg ttatcctttc aatagttaag aggaaagccc tcaagctaga
      901 tatattgacc gcgttacgga ttatcaagag gggctaacaa gattattttt tgttctttta
      961 ggttgattgt ggtactgttg atatacatgt atagcaaatg gtgttcggga ggtttatatg
     1021 cttgctatcc ggttatctga tgagattgag tcccgtctgg actcgctggc gaagcaaacc
     1081 ggcagaacaa agacgtttta tgcgcgggaa gcaatactgg cgcatctgga agacctggag
     1141 gattattatc tttcagcaga aactgctgca cgcgttcgcc gtggtgatga agcagtgcat
     1201 tcgtctgaag acgtgaggaa gtcacttggt ctggacgatt aactattccg atcgggcgct
     1261 caaatcgtta cgcaagatgg acaaacagaa cgcacgacgg attgtggatt ttatgagttt
     1321 acgcattgca gttgctgccg atcctcgcca gtcagggaag ccgctcaaag gtgagctggg
     1381 cgagttctgg cgctatcggg tgggagatta tcgcgttctg tgtgagatcc gagatgacga
     1441 gcttgttatc cttgccgcca cgattggaca tcgccgcgaa gtttacgact gaagcccgct
     1501 atgcgggctt ttttgatgcc gacaacaggt attatggtaa atactcgact aactacgtgt
     1561 tctactgttt gtcggtttta gttaaccagc aggttgtctt aacgataatg tagccgttat
     1621 ttttaactaa taaggtcata ttgttaacgt caggcataat aaaagacatt atggcctcag
     1681 agttatcatc agaagcagct ctctgcatta ctacttcgat tcccttttct ttgaatctat
     1741 aagtatccag ttgcctatta aacttagaaa gacctacaac catacttagt ttgttatctt
     1801 taacagtaag agatgcttcc tgagtaccat tacatcggta gtaagcggtg taaccgtcag
     1861 caaaagatcg accggggaat ataaatgcta ggaacgcaat taaccaatac atagttgaga
     1921 atcttggtat tacacatttc actatcctct ctttcatttt tcgttcacca tactttgtta
     1981 tctgattttt aatttttcta ttttataaac caagtctcta ccaaaaaggc gaaaattatc
     2041 ctcaaataat aataattgtt taagaacatc catgaaagca gctgcttcac cctcagttat
     2101 gggagctact ctatataaca tataacctga gttaagggtt tcttttatac tttcttcaat
     2161 gctcattata aatattagtg gattatcatt tttcttcccc catgggtttc taattaatcc
     2221 ttcgatgtac tcaggtcctt tgcgtcctat tgcctgcgca atatttttta tttcatctgc
     2281 gtgagggctt tgttgtacag ctttgtttat gcctgcttga gtgattttca gagactctac
     2341 accgtgtatg ttgtatgaga taaaatgtaa taaattaaga tgcaatgcaa ttaatccatt
     2401 tttgtttaca tctgagtatt tggataaaaa acgtaatgaa gtgttaactc tcccttgaag
     2461 cctaacaatt gggagtgctg ccttgtatat ttccttctcc gttaaatacc gtttaaaaaa
     2521 cataattctc cgcctccgta tagcgtgcct attttatttc gtcgtaaaac ttagctctga
     2581 aatattcctt tagaaccatc atgtcattaa taattgaatc tatctcatcc attttttctt
     2641 ggtattcttt taatggtgaa gggagcggta actcaatatt tgaaaagtga ttggcgagat
     2701 agggatcaag gtttctcatc cattcaggct tttcgccaat gcgttcttca actgaaagtg
     2761 aacctgataa tctctggtta ataagatcac ttagactcat tttggtttta ttcgaaagcc
     2821 gttgtaagga ttgctttaat cctatatcta gcatgatatt tgttctttct ctcattaccc
     2881 ccccttgcta ttgcgttttt tgttgcgcac aaagctacca ttgcgcatta agaaacgcaa
     2941 taaaaaagga gggagggagt ggtttgtaca tgatgtgcag tgcgctgagg gcatcagtgg
     3001 cgggtgtttt tccgtgggat ggatggagct ccggggataa cagaactgac gatcatttaa
     3061 aggatcttct tgagatcctt tttttctgcg cgtaatctct tgcactgtaa acgaaaaaac
     3121 cgcctgggga ggcggtttga tcgaaggtta agtcagttgg ggaactgctt aaccgggtaa
     3181 ctgggtatac gagaccgcag ataccaaaac tgttctttca gtgtagccgc agttaggcac
     3241 ccacttcaag acctctcaat atctctctcg tatatatcca gttaccaatg gctgctgcca
     3301 gtggcgtttt tgcgtgtccg tccgggttgg actcaagatg atagttaccg gaaagggcgc
     3361 agcagtcggg ctgaacgggg ggttcgtgca tacagcccag cttggagcga acgacctaaa
     3421 ccgagccgag ataccagcag cgtgagctat gagaaagcgc cacgcttccc gaaagggaaa
     3481 aaggcggaac aggtatccgg taaacggcag ggtcggaaca ggagagcgca cgagggagcc
     3541 gccggagaga aatcaccggc atctttaagt cctgtcgggt ttcgtcctcc tctgacttga
     3601 gcgtccattt ctgtgatgct cgtcagggga ggcggagcct atggaaaaac ggcaacgccg
     3661 catccttttc tccggccctt tctgatttga gcgcctttgc tttatcccgt tatccgtgag
     3721 tcggtcacgc ccgccgcagc cgaacggcag gagcgcagct accgagtgag cgaggaggcg
     3781 tcatttcatc tgtccgtgca tggtattgcg gacgctcctg agccacatat cacacctgcc
     3841 acatgaggca ctttcatgaa tcgtgctctc atgccaacat agtaagccag tatacacagc
     3901 gtaaacgctg tgactggttc agggctgcgc cccgaaaccc gctaacacca ctgacgcgct
     3961 ggcgcttgtc caacgccgga gcgacccgga aaacttttcc gtgccgtccg gcgtttgcga
     4021 aggagggata atgcagaagc cgaagaagaa gccgctcgtc gtaaaaaaaa gatcctggca
     4081 tccggcagtg atggcaaatc tgatgcgtca ggcacaggag gaagattacc gccgggagca
     4141 gatggcagag cttgagcgtt cagcccggtc acattttgcg gatatcggag gagaggatgg
     4201 cggttaagcg tacccgtttt ttaggtatcc gggtgaccga tggggagtac cagcagctgc
     4261 tggagcgctg caatggaagg caactggcgg tgtggatgcg ggaaacctgt ctcgatacgc
     4321 gcccggcgcg gtcgttgcgg ctgccgtcca tcgatccggt tttgctgcgc cagcttgccg
     4381 ggatggggaa caacctcaac cagatcgccc ggaaaattaa cggcggccag tggtcaggcg
     4441 ctgaccgggt tcaggtggtg gccgcgctga tggccattga tgccgaactc gagcggctgc
     4501 ggcatgccgt gctggaaaag ggggcagacg atgatcgtta agtttcatcc gcgagggcga
     4561 ggcggcggtg ccgggccggt ggattatctg ctggggaaag accgccagcg cgacggggcc
     4621 atcgtcctgc agggaaagcc ggaggaggtc cgggagctta tcgacgcctc gccctacgcc
     4681 aaaaagtaca cctccggcgt cctgtctttt gctgaacagg atttaccgcc cggccagcgt
     4741 gaaaagctga tggcgagctt cgagcgggtt ctgatgcccg gactcgataa agaccagtac
     4801 agcgtgctgt gggttgaaca ccgggacaag gggcggctgg aactgaattt tctggtaccg
     4861 aacacggaac tgctgaccgg caggcgtctc cagccgtact acgaccgggc agaccgtccg
     4921 cgcatcgatg cgtggcagac cgtggtgaac gggcgactgg ggctgcacga cccgaacgca
     4981 ccggagaacc ggcgcgcgct ggtgacgcca tcttcgttac ccaaaacgaa gcaggaagcc
     5041 gcagaggcta ttacgcgggg cttactggcc ctcgcctcgt caggggagct taaaacgcgt
     5101 caggacgtca ctgaggcgct ggaaagcgca ggttttgagg tcgtgcgcac cacaaagagc
     5161 agcatcagca ttgccgaccc ggacggaggg cgaaacatcc gacttaaggg agccatctat
     5221 gaacagtctt ttaacgctgg cgaaggactt agagcagaaa tcgaaagcgc agcagcagag
     5281 taccggcgaa atgctgaaag ccgcattcag cgagcacgag aagtctgtca gagcggaact
     5341 gagcgaaagc gggaagagaa tcagcgccgc catccgcgcc cacgagcagg gtatgagcgc
     5401 ggccatgcag tcgaaccgcc tgagcgtgat gcgtatggtc gggcggacat ggctgaccat
     5461 cacgatggtg tcaggactgc tgttcgccag cctgagcggg gtgttgtggt atcaggggag
     5521 cttgatagcg tcaaatctgg ccgaaatcga ccggcagaac gcagcgctgt cgaagctgaa
     5581 cgcgaagacc tggggc
//
