LOCUS       NZ_CP040715            11970 bp    DNA     circular CON 25-JUN-2023
DEFINITION  Klebsiella pneumoniae strain 16HN-35 plasmid p16HN-35_4, complete
            sequence.
ACCESSION   NZ_CP040715
VERSION     NZ_CP040715.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN11867826
            Assembly: GCF_030323825.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Yang,X.
  TITLE     complete sequence of Klebsiella pneumoniae strain 16HN-35
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   Yang,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2019) The Department of Applied Biology and
            Chemical Technology, The Hong Kong Polytechnic University, Hung Hom,
            Kowloon, Hong Kong 999999, Hong Kong
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/23/2023 17:46:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,828
            CDSs (total)                      :: 5,708
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 252
            CDSs (without protein)            :: 252
            Pseudo Genes (ambiguous residues) :: 0 of 252
            Pseudo Genes (frameshifted)       :: 159 of 252
            Pseudo Genes (incomplete)         :: 118 of 252
            Pseudo Genes (internal stop)      :: 30 of 252
            Pseudo Genes (multiple problems)  :: 54 of 252
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP040715.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="16HN-35"
                     /isolation_source="sputum"
                     /db_xref="taxon:573"
                     /plasmid="p16HN-35_4"
                     /geo_loc_name="China"
                     /collection_date="2016"
     gene            277..522
                     /locus_tag="FGL96_RS29025"
                     /old_locus_tag="FGL96_29400"
     CDS             277..522
                     /locus_tag="FGL96_RS29025"
                     /old_locus_tag="FGL96_29400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            796..1167
                     /gene="tolA"
                     /locus_tag="FGL96_RS29030"
                     /old_locus_tag="FGL96_29405"
     CDS             796..1167
                     /gene="tolA"
                     /locus_tag="FGL96_RS29030"
                     /old_locus_tag="FGL96_29405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            1164..1529
                     /locus_tag="FGL96_RS29035"
     CDS             1164..1529
                     /locus_tag="FGL96_RS29035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            1704..1982
                     /locus_tag="FGL96_RS29040"
                     /old_locus_tag="FGL96_29415"
     CDS             1704..1982
                     /locus_tag="FGL96_RS29040"
                     /old_locus_tag="FGL96_29415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            1970..2284
                     /locus_tag="FGL96_RS29045"
                     /old_locus_tag="FGL96_29420"
     CDS             1970..2284
                     /locus_tag="FGL96_RS29045"
                     /old_locus_tag="FGL96_29420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            2448..2876
                     /locus_tag="FGL96_RS29050"
                     /old_locus_tag="FGL96_29425"
     CDS             2448..2876
                     /locus_tag="FGL96_RS29050"
                     /old_locus_tag="FGL96_29425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            2902..3081
                     /locus_tag="FGL96_RS29055"
                     /old_locus_tag="FGL96_29430"
     CDS             2902..3081
                     /locus_tag="FGL96_RS29055"
                     /old_locus_tag="FGL96_29430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(3108..3638)
                     /locus_tag="FGL96_RS29060"
                     /old_locus_tag="FGL96_29435"
     CDS             complement(3108..3638)
                     /locus_tag="FGL96_RS29060"
                     /old_locus_tag="FGL96_29435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(3645..4376)
                     /gene="mobC"
                     /locus_tag="FGL96_RS29065"
                     /old_locus_tag="FGL96_29440"
     CDS             complement(3645..4376)
                     /gene="mobC"
                     /locus_tag="FGL96_RS29065"
                     /old_locus_tag="FGL96_29440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(4376..6340)
                     /locus_tag="FGL96_RS29070"
                     /old_locus_tag="FGL96_29445"
     CDS             complement(4376..6340)
                     /locus_tag="FGL96_RS29070"
                     /old_locus_tag="FGL96_29445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(7824..7973)
                     /locus_tag="FGL96_RS29075"
                     /old_locus_tag="FGL96_29455"
     CDS             complement(7824..7973)
                     /locus_tag="FGL96_RS29075"
                     /old_locus_tag="FGL96_29455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(8058..8315)
                     /locus_tag="FGL96_RS29080"
                     /old_locus_tag="FGL96_29460"
     CDS             complement(8058..8315)
                     /locus_tag="FGL96_RS29080"
                     /old_locus_tag="FGL96_29460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(8325..10010)
                     /locus_tag="FGL96_RS29085"
                     /old_locus_tag="FGL96_29465"
     CDS             complement(8325..10010)
                     /locus_tag="FGL96_RS29085"
                     /old_locus_tag="FGL96_29465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            complement(10115..11602)
                     /gene="ltrA"
                     /locus_tag="FGL96_RS29090"
                     /old_locus_tag="FGL96_29470"
     CDS             complement(10115..11602)
                     /gene="ltrA"
                     /locus_tag="FGL96_RS29090"
                     /old_locus_tag="FGL96_29470"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     BGC             3325..11970
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          7143..7721
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3649..4376)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 tacatataca cagtataatt attaccttac tcctcccgtt ttcaggtgtc aatatatgag
       61 attagttctc atcttcaggc acaaaaaagc gcccggagcc acctgtaagc agatcgcgtc
      121 ttttcccgaa aatttctcat ggcaacaaat tttattattt aagacattca tcacacttac
      181 atttcagatg gtatagacac atctgaaagc taactggcat gaggagagga cctgcggtta
      241 aactgttcgg gaaacctgag acgcccggag gccactatga cgcacctgaa actgactccc
      301 gctgaagaaa agcgcctggc ggacgccttt gtcgctcagg cactggccat gggcctgaaa
      361 gggaacaaaa cgctgccaaa ggcggtaaaa caacaggtgc gggaaagggt gcttcgtgcc
      421 attcaggacg aacgtgaagc caggcgggcg caggaaatgc gtcgggagaa atacgaggct
      481 acggaaacca ccttcacctg gcagccacag cgccgccggt aaggggtatt taccataacg
      541 tcgcattgcc cgcaccgctc agacggtctg agggcgtttc cggcggctac gggataaaac
      601 aggcaacaaa ccacccggaa aaccgccgga aacgcgcccg gtaaattgta acccgctgca
      661 tgcctatgca cgcaactgca tggcgggccg tttggtgtaa aaacgatacg ataagccgtg
      721 tttatgtaaa tgaacgtggc cgtaaagagt gacaaatata catgtaacat tttttactga
      781 caggtcatca acccgatgcg tttaataccc tttatcctgc tggcattgtg cagcccctta
      841 ctcctgacgg cctgtaagca ttccaccacc cagccgccga aaatttatac tctggataag
      901 tcatctaaaa tcagtagcta cgctgcggcc atcaagaccg ctgttgaaat gcagatgttt
      961 gaaccagagg cctatgcggg aaaaatgtgc accgtgcgga tatccataca acgtgatggc
     1021 actctgaaca gcgccattgc tgaaggagga gatccgaaac tctgtaaagc agcaatatca
     1081 gccgttaccc acgccaaaat tccgccggca ccggatgaaa atacctggca aatattcaaa
     1141 aacgcccctc tggatttcag accatgaaag cttttactct gacagtgctg gcggccatgt
     1201 tcctcactcc ggtacatgcg ataccactaa aacccgtaac cacactgcag ccaacctacc
     1261 cggtacaggc cgcagcaaaa cgcatcgaag gtcaggtgac cgccacgttt gatattctgg
     1321 acgatggacg agtggacaac gtgtcgctta ccggctgtca cctttttttt cgggaaacgc
     1381 tgacggcaat gaggcactgg ctatatcccg cagggcaccc ggcaatgcga caggttataa
     1441 attttgaatt cagtctgaaa aatgacggct tacaggatcc gcgaccagtc accacccaaa
     1501 agctcaatga tgatggttgc cgccagtaac aggtccttta acccgcaggc tgcaatagtc
     1561 ctggatgatg agtgagcctg tagcagccct gattagtgga agcgaagctg aaaaatgggc
     1621 agcaaaagcc gtatttctgg caaatagcgc cgccacggat taaagtagtc atgtgtaata
     1681 catatgacta caaaggggct tttatgtcat cagcaacatc catcaggctc gatgaagaac
     1741 ttaaggaccg gctgaaaacg ctggccgatg accggcaccg ctcagcgcac gcgctaatgc
     1801 tggaggcaat caccgaatac atcgaccggg aagagaagcg cagccagtac ctgcgggacg
     1861 gacaggccgc atggcagcac tatcaggaaa cgggtctgca ccttactgcg gaagaagcgg
     1921 aagcctggat aagtacctgg ggaactgaga acgagcagga tgctccgcca tgccacaggt
     1981 aacgatttcc gcgctggcac agcgcgattt acaacgcctt caggactttc tcaaaaccaa
     2041 aaatcggctg gcagccagaa aaggaggtga ggtgatcgtc cgggctatcc agcaactgaa
     2101 aacattgcca gacattggcc gcccggtgcc gtttctgccg ctggaatata aggaactggt
     2161 gatcgggttt ggcgacagtg gctatgtgat gctctaccgc cacgaccggg aaatggacca
     2221 gattgtgatc gtcaccgtca ggcatcagaa agaatccggg tatccggggg cagacagcct
     2281 ctaataaccg atgacggggg cgcaattcat tgcgtggccg tcgccggttt gacaagcgca
     2341 gcgcgtcaga aaaccacatc ctgaacggaa aaacgggtaa aattcagcga tacatttacc
     2401 gtgagaaatt acacaccttt cacacccgat aaaatcaggg gtatacagtg gcgaaaacat
     2461 tgctcaacct ttcacaggca gcacaagccg cgggcattac cagaagaacg ctatacaatc
     2521 atgtaaaaca gggaaaggtt accgtgtcac gtgacgggaa aaacaaccct gtcgtggatg
     2581 tgtcagaact gatccgtgta tacggaaatg tgaatatccc tgagaaacaa attcccggca
     2641 tttcacaccg tgaaaataca caaaaaaatt tcccacagga acagcttctg gcaatgcaaa
     2701 aggaactggc cgatctccgg caggctgtca ccctgatgct ggaagataaa acatcccggg
     2761 aagaagaacg ccgccagcac gatgatgagc gccggaaact ccaggccgaa gtggacagat
     2821 tgacaacaga actgacacag aagaaaaaac gcttctggtc cggctggttt agctgagaac
     2881 ttaccgaagg gaatggagaa catgacgaag caggaaaaaa ctgccctcaa tatggctaaa
     2941 ttcattaagg atcagtcgct gctgttgctg gaaaagctga acgagctgga catggatacc
     3001 ccggccgaca tgtgcgaacg gcttcacgag gatgcggaaa cactctggca ggtgatgcgg
     3061 gataagctcg gggaagaata acagcaccgg ccatgccggt gctggcgtta ttttttgccg
     3121 aacggccacc accggcgggc gggtttgtcc tccggcagtt caggcaccgg cacgttaaaa
     3181 tagcggctgt tatattcccg catcagctcc gctttcagct tctcgttcac cgcgctttcc
     3241 ccgtgccgct cgctgaccac ctcaatcatc gcgttaatct gctccggtga gcacttcccg
     3301 gcatcaacca cctgcacacc gttctcgatg gcgaaccgct ccacctttcg atagaggtaa
     3361 tcacacagct tcattcctgt gggtttgtcc cgttcccatt gcggattttc accggtgaaa
     3421 aagtacgtgg ctttttcgtc gctgttgagc cgctcgtcat acatcccgat ggatttcatc
     3481 atcgccagat gctgggcccg gtcgacaaaa cagaaaacat acgccccggt ctgccaggcg
     3541 agcaccacca tgtcttccag atgctcgctg gttggcataa tgcagcgctc gccgtctttg
     3601 tcttccacgt cctcaataaa taccatgtac agcgccataa tccctcactc cgttgtcata
     3661 aatttaaagc acgcgaacag cctctcccgg tccagctcca ccgttttgcg ggcctcgccg
     3721 gtctgtggat tcacgatttc atccaccgcc agaatcgccc gctgtacccg gacaagcgtc
     3781 ggccggtcag ggcagatgta ccagatttca tcgatgccgt tcgcccggcg gttgaacaga
     3841 tacccggaca ccacttcctg ataccgcctg cggctcttga cggtgcgctc tacctcaaat
     3901 gccaccgttc tgccgtccag ctccgccagc gcgtccggca ctttcatccc tttcagggcc
     3961 atacggtgca ggtaccgcca gccggtggcc ccccggccct ccatggagag gcgcagggac
     4021 tgcacggcca gccggtgcgg cagctgcgcc gccgacaccc ttgacggctg aaacggaata
     4081 acgtccgtca gcggctcgtc ctcgtcgaac gagaacgcca ccccgtgcgg cgtcagcccc
     4141 cacacgctga cctgacggcc gtcaaccggg tatttcgccc ggaccagcag ccctttcgcc
     4201 accagactgt ccaggaagcg gtaaaccggc tgtaccgccg caatccccag ccgtcgcgca
     4261 accagctccg ccgtggaaca cagctcttcc cgcagccagc gcagggtcag ccgtgttttc
     4321 tccgcgttcc gcgcctgccg cttcgcgtgg ctgacgttgt actgctccag tgtcatcagt
     4381 aaagccccgc aaaatcatca tccgcttttg gtccgccgcc gtcggccgga ggcaggtact
     4441 ccgccagcgg gtccgttttc gccagcaccg ggaaggtttt cagcgaaatt tcccggcgct
     4501 caacccgcag cgggctgctg aacgccagcc gcgccacgcc cagcccggtc atcaccgcga
     4561 acccgtcagg caggtgttgc agggtgttca cgtcaaacag cggccgcgcc tccttctgga
     4621 tgaagctgtc gccgctgatg tgctccacgt tccccgcctc ccggctggcc cggcggcgct
     4681 ccacgtccac ccggatctcc ccggtctggc ccgccgccca ctgcgcggac tcctggctgg
     4741 ccgcgcgata gaaccagcgg atcggcgtgt tatccagtac cgtggttttc acaaaatcag
     4801 cgggcagatc ctgcccgcac tgaccgaagt cgcccagcga ctggtgcgcg agcagcaggt
     4861 tgcagtcccg gctgcgcagc gtccccagcg cgttcagcac gtacttcgac agcagatatt
     4921 taatctcatc gagcatgatg ctcgcgtgcg gccacctccg gaactcatcc cgcgcaatga
     4981 tgatttgcgc gcagcgggca aacagcatct tctgcacccg gatcaccgcc tcatcgtcca
     5041 tcgagccgat gacgtacagg cagccgccgc cgttgattat ccccgccaca tcgataccgc
     5101 tgtccgtctg caggacgctc aggtcggcca cgttctccag cttggtgatc agccccttca
     5161 cgtccttttt cagggcctcc ggcaacgcgt acgccgccgc cagcacctgc tgaatgtttg
     5221 ccccctgcgg gaactgctcc gcaatcagct tcgccgcttt ctgctcgctg atgcggtagt
     5281 ggtccgccac gtcgccggtc tcactcaggt taaacccggc aaccagcaga ttcttcaggg
     5341 cgtactggtc gagatcgcgg aacagattca gctgcgccgg tttccccggc cgcagatcca
     5401 gcagggtgaa gtccgggcag tgcgcccgga agacgtgcgg cgcccacgcg tcccctttgg
     5461 ggtcgaagta caccacagcg tccccgaaat cgcgtacgca gcgaatgagc gcgttggtgg
     5521 ccatcgcgct cttgccgctc ccgggcaggc cgaggatctg aatattggtc ttgcgccagg
     5581 tatcacgagg gaccagcacc ggctggccct gctcatcctt acccagaaac accgcctgct
     5641 gtttccgggc ctgctccgtg gaaaaatcgg ccagcgcgtt aaccggtacc ggctccccga
     5701 aatgcaggtg gcggacgtct gtcatttctc cggtccgctg gctgctgcgc cggctctcct
     5761 gattcagttt cgcgttcacc gcctcgtcag cggccgcaaa atgaccatca agcagcagga
     5821 acgccagcag ccgcgccaga aggacgccgg cgacaagccc tccggcacac caggctatcc
     5881 cgaacaggtt agccggctgc agcaggtaat aatctgcgtt cgcatagagg tccgggcgca
     5941 ggacggtttc tccccagctc tcgataaaca cggcgacaac cagccccccc agaagggcca
     6001 gaccggcagc gccggtcagc cagtacgcgt agttccagac atgtcgccgt gccctgacca
     6061 tccgcccggg tgaatccagc gaggccagca ccgaaaatgg cacagtccgg ttattatcgc
     6121 gcttatagcg atcggcatac tcccgccgcg ccttatggct cagccccaca aaacgcattc
     6181 ggccggccca gagttcagcc agaccgaaaa acaggccgat aaccagaaaa aaaccggcca
     6241 gactgtaata ctcccggtac agccagctga cccacgggta aatctgccgg gccagctccg
     6301 ggatatgcca ctgccaggca agcgaatccg tattaaacat gcaatctctc tccttcagac
     6361 gacgggttta ccggcgtgat gcgcagcccc cgctgcacaa gccgcggaca tgccccaagg
     6421 catggcggcg gcggtcacgt ccgtggtgat tgcgttcagc tttcaccgcg gaggccagcc
     6481 tctttccgcc ccatgcggaa aaggcgcagc cccggaggaa caacgagcac gccccagcgt
     6541 gtgagaggaa aaggcggcac cccagtgcgg tcttttccgg tgttccggcc cccgtcagag
     6601 ttcgccatag cgagcggctg accacccgca aaatcaggag cagctttgct gcttcgtcag
     6661 gtgcgtccag caccgtggat ttttgtgggt ggtcaggcgt gagcctgacg ggggccgggt
     6721 gaatttctgg cgtggccggc gtccagccgg acagagccag aaagagggga aaagaccgtg
     6781 cgtcggtggc gtccagccag cgtaagcaag gtcttttccc ctgaagtcag ccacgcccca
     6841 gcggggatga cttgcatttc tccgcaagga gaaaaaagca acagagatag gttaacgtaa
     6901 aatgttcagc gaaaaaccga catggttcac ctatcctgat aattgatcgt cagggaatag
     6961 aaagacttaa tcaggggaca atatcccaca tcagcgcgtc cggtgaccgt cttgcaggtg
     7021 ccggggaaat tgcgcctcct cgctcactcg gtcgctacgc tccgggcgtg agactgcggc
     7081 gggcgctgcg gacacataca aagttaccca cagattccgt ggataagcag gggactaaca
     7141 tgtgaggcaa aacggcaggg ccgcgccggt ggcgtttttc cataggctcc gccctcctgc
     7201 cagagttcac ataaacagac gctttttcgg tgcatctgtg ggagccgtga ggctcaacca
     7261 tgaatctgac agtacgggcg aaacccgaca ggacttaaag atccccaccg tttccggtag
     7321 gtcgctccct cttgcgctct cctgttccga ccctgccgtt taccggatac ctgtccgccg
     7381 ttctcccttg cgggaagtgt ggcgctttct catagctcac acactggtat ctcggctcgg
     7441 tgtaggtcgt tcgctccaag ctgggctgta agcaagaact ccccgttcag cccgactgct
     7501 gcgccttatc cggtaactgt tcacttgagt ccaacccggg aaagcacgca aaacgccact
     7561 ggcagcagcc attggtaact gggagttcgc agaggatttg tttagctaaa cacgcggttg
     7621 ctcttgaagt gtgcgccaaa gtccggctac actggaagga cagatttggt tgctgtgctc
     7681 tgcgaaagcc agttaccacg gttaagcagt tccccaactg acttaacctt cgatcaaacc
     7741 acctccccag gtggtttttt cgtttacagg gcaaaagatt acgcgcagaa aaaaaggatc
     7801 tcaagaagat cctttgatct tttctactga accgcgatcc ccgtcagttc agaggaggac
     7861 gatggtgcca ccgttccgcc ctgaacatcc cggatataat ttgcctgaca tgcggccaga
     7921 agaaagccag caacaatcaa tataaaaaga aaaatagtct ttaccttttt catatacttt
     7981 ccctgtattt aaagactccg gcattcatcc ctgaacaccg gaagtcagta tttcaaatca
     8041 tccttgataa ttttttgtta ccagttaccg tcccgataat caaaagctat ataacaatta
     8101 tccacatcag gttttatttg atttttaaaa aatggctgta gtaaaggaac ccattctttc
     8161 ttcaaatcaa actcaccatt attaatatta tcctttaaag gcattcccaa gctttcaatg
     8221 acagaaccat cgtcaccaaa gtctttagaa tactcttccc ctttaaactc ttcagttttc
     8281 ttatcaaacc atgtcaaacg taatttaagc cccatattta cctctcaaag atatttttta
     8341 atgttccgtt ttggatctgg ccctttaacc tgcttacccg ttttgggatc aaatgcgcct
     8401 aggtgttcgc catcactggc acgataccct tcaagctcac cgtgctggga atcccattca
     8461 taaattttac gttttttatc gccataccat cgggcccgct taccaccacc accttgttta
     8521 ggtgttttag gatcacctcg ctttaactct ccaagtccct ttatgtcttc tgttttaggt
     8581 actggatgat aatcatggcc gtagtcttta gtccccttgc gaggcttgtt cttttcctca
     8641 tttaacttat tttctgcatc cttcgctttt ttctcctttt gtttccggct ctccatcgcc
     8701 ccggtaagtg cagccttatc tctggataag gattcattca ttgcgtttaa agatgcctga
     8761 gccgctttta atttctcatt ctgattctga atatccttat caagatagcg aacctgatcc
     8821 tgccacccgg agccatattc atgaggcggg ttataagtca caaaatcatc tttatttgcc
     8881 tctgcctctt ttaccttttt atcggcttcc tgaacagcag cccttgcacc ggggagcgcc
     8941 ttctctgtcg atgcaatgcg gctgtttaag gtcgcaatat ttttatcttc ggcatccagc
     9001 tcagctttcg ctttatcata gtctcttttc agcccctctt ccggatgagc ggcgtcccat
     9061 gcctgctggc gacgcttttc ttcctcctga cgctgtttta cctgtgccgg ggtaagaaca
     9121 tctgtcaccg acacataaac cggcttctgt ccggtctctt tcgggaaacg aataacggcc
     9181 tcacgggagt taccaccggc tgtaaaacca gccggacgtg aatcaccttt ttcagcaaca
     9241 atgccttttg gcggggcttt cgctgtcgga acacctttcg gtacgctcac ctgcagagac
     9301 gggagacccg gaatcgacac actgaatacc cccggacgtt ttgtcggttt cgcatccacc
     9361 acagggacac tcattggccg acctgcgaca accgcaatat gctgccgctc atccttcacc
     9421 acatccacaa cccgttgtct gacacgaacc gtcgcctggt ccagtggtaa agtacttgcc
     9481 ggagtctccg tcaccgcatc ggccggtaat gacgtcataa tttttgacat cattttcgga
     9541 tcatcttttg caatctcaga aggcagcaca ccgtagatcg caatccccca cagaccaaac
     9601 ttaaacggcc ccttcagggc agccagcaca tcagcaacgg cggccgacaa cgcatcgccg
     9661 gaaactgata aagccagtcc ttcagccccc ggagtagcca gggcaggcag accgaaggcc
     9721 atcgcagtgg ccgaagactg tccgccacca gagctaccgc tatgattgcc gctgttacca
     9781 ccattaccac cgggtttact cccgccgtta ccaaactccc ccggattata gttatgaata
     9841 tctccattcg gcgtatgcgt cgtaccccag cccggcccgc tgtttggatc cgtaccacca
     9901 cctgatgaag ggccaccttt accagacgta ccgttcacat tcccactggc ctgaccacca
     9961 ttatgtccca gacctgaatt acccggacct cgaccgtctc caccactcat accgttttcc
    10021 tctttgacat ttaaaacaat aaattaaagc caacatgtcg cggtagggat acccgttacc
    10081 ggataccccc cgcacagatc ccggcgtgcg cgatttacgc accgggctcc tgcctcgggt
    10141 gtctggcggt gaaccgctcc acaggccatg gatgaagaac ccgaaccctt ggtagccatg
    10201 cggctgccag tttgtttgct ttcgtccagg tcgtatcatc cttctggctc ctgcgcctga
    10261 gcgcccggcg ccagaggttt gttacgtgtg tcctgaactt ctgcatggtg gggaagttgc
    10321 ccggtaccga gtgatagttc aggtatccct gaaccactct cctgagccat tttccctgtt
    10381 cggggattga gtaatgccag cgccttcgca gaccgtcttt gatggctttc agagttgccg
    10441 tcatccgatc ccggcgggtc tttcgtatca gcatgaacct gccgttgcga tctttcccgc
    10501 tgatgtgcgt gaacccgagg aagttgaacg tttctggttt gccttttccc ctgatggcac
    10561 ggttttcggc agcgaagcgg ccgaactcca tcagacgggt tttctccggg tgaaccgtga
    10621 gtccgaactc cctcagtctg cgctgcatgg ctatacggaa gcgccgggca tcgtatcgtt
    10681 tgtcgaaccc gatgacgatg tcatcggcgt atctgaccat taccacattg cctgtggcat
    10741 agcgacgtcg ccactgatgc gcccacagat cgaagacgta gtggaggtat atgtttgcca
    10801 gcagcggtga gatgaccgca ccctgtgggg tgccttcctc cgttgctcgc cattgaccct
    10861 cctccgacgt cccggctgtg agccacttac gtatgagcct gattaccctc cggtcgccga
    10921 tccgatgctc tgtgaacctg atcagccatt cgtggctcac cctgtcgaag aactgactga
    10981 tgtcggcatc cagtacccag tttacgttag tgcgtaccag ccctgtggcc agtgcgtcca
    11041 gtgcatcgtg ctggcttcgc ccgggtctga acccgtatga gaaccccata aagtcgtttt
    11101 catagactgc gttcaggatt ttcaccagcg catactggac gatcttgtcc tccagcgagg
    11161 cgatgccgag cgggcgttgt tttccatccg cttttgggat gtagtgacgc ctgccgggct
    11221 gcgccctgta gctgccctga tgtagcctcc ggtgcagatc tgttatgttg ttcttcatgt
    11281 ttccggcgta gtccatccac ctgatgccat ccactccggc ggccgctttc ctgctcaggg
    11341 agaggaatgc ggcttccagt gcttcgactg tcagcaggtg gaacaatgct gtaaaccgtt
    11401 ctttcttccg ctgcttcgca gcttcccgca cgcgtgacag cctctgtgac atgctttccc
    11461 ggctctgtgt ccggcgcatg tgtggctgtt ccgcgttccc cttggccccg ctccttcgct
    11521 ccactgactc cgctcctttc gggttgttcg cctgcttcgc cgctactatg agcgagtccg
    11581 acttctcctc tccgtacatc accggctatg actcctcgtc ttcccggtgc gggccatctc
    11641 cgacactggc agatggtcag aggggagatc tcccggttcc cgcgtagaga tcgtattgac
    11701 atgccagggt ctcagacccc gccgggtcca tgtggcactc gcagtatcgc accctatgat
    11761 gttgccttcc gttaacagta caacgtcggc acccggtaat ttaatataca tttcgtggct
    11821 caatggctgg cctgtcaaca cccctgtcaa cgcttcgccc catacctcgc ggtatgcaac
    11881 gcatgactcg gggaccttgt ggattgctgg tccttcaatg gtcggggact ttcacccctt
    11941 gatctctaac cggtctcccg gcgcacactg
//
