LOCUS       NZ_CP040705             8748 bp    DNA     circular CON 25-JUL-2023
DEFINITION  Enterococcus faecium strain HOU503 plasmid p4, complete sequence.
ACCESSION   NZ_CP040705
VERSION     NZ_CP040705.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN11848047
            Assembly: GCF_005952885.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8748)
  AUTHORS   Shamoo,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAY-2019) Biosciences, Rice University, 6100 Main St.,
            Houston, TX 77005, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. Mar-2017
            Assembly Name                     :: HOU503F_del_liaR
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 10.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/25/2023 04:46:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,745
            CDSs (with protein)               :: 2,745
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 106
            CDSs (without protein)            :: 106
            Pseudo Genes (ambiguous residues) :: 0 of 106
            Pseudo Genes (frameshifted)       :: 38 of 106
            Pseudo Genes (incomplete)         :: 59 of 106
            Pseudo Genes (internal stop)      :: 27 of 106
            Pseudo Genes (multiple problems)  :: 15 of 106
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP040705.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="HOU503"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="p4"
                     /geo_loc_name="USA: Houston"
                     /collection_date="2015"
                     /note="liaR deleted isolate"
     gene            complement(join(7918..8748,1..138))
                     /locus_tag="FGF98_RS01280"
                     /old_locus_tag="FGF98_01280"
     CDS             complement(join(7918..8748,1..138))
                     /locus_tag="FGF98_RS01280"
                     /old_locus_tag="FGF98_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_002298422.1"
                     /translation="MSKNGIIVNEWENLTALSNRRLNVQNMYTPLKMQWSIDRITIVGK
                     LKENIYYHTQNDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQYEENIAYIEILKWQD
                     GKGRIDFNPNKIGHFLASSMKNFIHDLFLEPHFSRADIACDIIDVPDEFITQYRVVDPV
                     SFKPIYGRSGKLETAYWGSRASERQIRLYNKKLEQETKRKIVPPEIETWWRLELQLRRG
                     KATDWHAMVHESLNSFASPHFLPLDVKTNDKVMIFGLIANPEMWGFLERRMKYKLRDIL
                     KRESQNDELTNHLRETFSESADVLKKELDTWLQGLDVTEEE"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            complement(585..1991)
                     /locus_tag="FGF98_RS01285"
                     /old_locus_tag="FGF98_01285"
     CDS             complement(585..1991)
                     /locus_tag="FGF98_RS01285"
                     /old_locus_tag="FGF98_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_002317172.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDENI
                     YLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIGIL
                     DDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDEISP
                     HLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIERLMN
                     DYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYISRQREENERDK
                     ALMASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDFVDLYQR
                     ATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQVENKKLKE
                     ENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFYRQNVSQRK
                     LERSEDEIEW"
                     /name="plasmid recombination protein"
     gene            complement(2213..2575)
                     /locus_tag="FGF98_RS01290"
                     /old_locus_tag="FGF98_01290"
     CDS             complement(2213..2575)
                     /locus_tag="FGF98_RS01290"
                     /old_locus_tag="FGF98_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298418.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKRF
                     GFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKKSQ
                     MSESKFTSIFNDVNLR"
                     /name="hypothetical protein"
     gene            complement(2579..2887)
                     /locus_tag="FGF98_RS01295"
                     /old_locus_tag="FGF98_01295"
     CDS             complement(2579..2887)
                     /locus_tag="FGF98_RS01295"
                     /old_locus_tag="FGF98_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298419.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERMMDVLN
                     SVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM"
                     /name="hypothetical protein"
     gene            complement(2896..3315)
                     /locus_tag="FGF98_RS01300"
                     /old_locus_tag="FGF98_01300"
     CDS             complement(2896..3315)
                     /locus_tag="FGF98_RS01300"
                     /old_locus_tag="FGF98_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298420.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVIM
                     LDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELTEM
                     IRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
                     /name="hypothetical protein"
     gene            complement(3308..3541)
                     /locus_tag="FGF98_RS01305"
                     /old_locus_tag="FGF98_01305"
     CDS             complement(3308..3541)
                     /locus_tag="FGF98_RS01305"
                     /old_locus_tag="FGF98_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298421.1"
                     /translation="MAEKQKHFGKKNWLVNLTGDNVVVVFLDSKKMSGKLLDVTQYELI
                     LKNNKIDSPIIIPKHAVKYVYGVAESNGEDND"
                     /name="hypothetical protein"
     gene            complement(3544..4512)
                     /locus_tag="FGF98_RS01310"
                     /old_locus_tag="FGF98_01310"
     CDS             complement(3544..4512)
                     /locus_tag="FGF98_RS01310"
                     /old_locus_tag="FGF98_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_002298422.1"
                     /translation="MSKNGIIVNEWENLTALSNRRLNVQNMYTPLKMQWSIDRITIVGK
                     LKENIYYHTQNDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQYEENIAYIEILKWQD
                     GKGRIDFNPNKIGHFLASSMKNFIHDLFLEPHFSRADIACDIIDVPDEFITQYRVVDPV
                     SFKPIYGRSGKLETAYWGSRASERQIRLYNKKLEQETKRKIVPPEIETWWRLELQLRRG
                     KATDWHAMVHESLNSFASPHFLPLDVKTNDKVMIFGLIANPEMWGFLERRMKYKLRDIL
                     KRESQNDELTNHLRETFSESADVLKKELDTWLQGLDVTEEE"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            complement(4959..6365)
                     /locus_tag="FGF98_RS01315"
                     /old_locus_tag="FGF98_01315"
     CDS             complement(4959..6365)
                     /locus_tag="FGF98_RS01315"
                     /old_locus_tag="FGF98_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_002317172.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDENI
                     YLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIGIL
                     DDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDEISP
                     HLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIERLMN
                     DYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYISRQREENERDK
                     ALMASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDFVDLYQR
                     ATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQVENKKLKE
                     ENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFYRQNVSQRK
                     LERSEDEIEW"
                     /name="plasmid recombination protein"
     gene            complement(6587..6949)
                     /locus_tag="FGF98_RS01320"
                     /old_locus_tag="FGF98_01320"
     CDS             complement(6587..6949)
                     /locus_tag="FGF98_RS01320"
                     /old_locus_tag="FGF98_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298418.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKRF
                     GFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKKSQ
                     MSESKFTSIFNDVNLR"
                     /name="hypothetical protein"
     gene            complement(6953..7261)
                     /locus_tag="FGF98_RS01325"
                     /old_locus_tag="FGF98_01325"
     CDS             complement(6953..7261)
                     /locus_tag="FGF98_RS01325"
                     /old_locus_tag="FGF98_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298419.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERMMDVLN
                     SVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM"
                     /name="hypothetical protein"
     gene            complement(7270..7689)
                     /locus_tag="FGF98_RS01330"
                     /old_locus_tag="FGF98_01330"
     CDS             complement(7270..7689)
                     /locus_tag="FGF98_RS01330"
                     /old_locus_tag="FGF98_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298420.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVIM
                     LDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELTEM
                     IRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
                     /name="hypothetical protein"
     gene            complement(7682..7915)
                     /locus_tag="FGF98_RS01335"
                     /old_locus_tag="FGF98_01335"
     CDS             complement(7682..7915)
                     /locus_tag="FGF98_RS01335"
                     /old_locus_tag="FGF98_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298421.1"
                     /translation="MAEKQKHFGKKNWLVNLTGDNVVVVFLDSKKMSGKLLDVTQYELI
                     LKNNKIDSPIIIPKHAVKYVYGVAESNGEDND"
                     /name="hypothetical protein"
     Typing          complement(3552..4429)
                     /name="rep_cluster_943"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="83.973"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          complement(7926..8745)
                     /name="rep_cluster_943"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.058"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          complement(589..1991)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.88"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4963..6365)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.88"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cagttttcca acgatagtaa ttcggtcaat tgaccactgc atttttaagg gggtgtacat
       61 attttgtaca tttaaccgcc tgttagatag ggctgttaaa ttttcccact catttacgat
      121 tatgccattt ttactcaaat tttgttcctc ctcaaagaaa aagagacttc tggaaagaat
      181 tttcaaaagt tcacgtaact cttaaaaatt cattaccaaa aaatctctag tcttgaagaa
      241 atctaaaaat attcgtataa tgattaagaa gttacggagc tagtgcgatt agtttccgta
      301 aaggtcgaga acgttggtag cgttgctcga ctattttttt actctttttt cagttcattt
      361 gtttgctaac tcaataatag cacttctctt tagctatgca aatatccaaa aaataaaacc
      421 gcaccctttg aaaagggcgc ccccaaactt cgctaacgct aggttggcca aaaaatggac
      481 tggccatttt ttagcaaatg tcagcttaat aggctagtgg tttagccgtc aactccttat
      541 cctcggcaca aaccgcctcc ggtaccgttg actgcacatc gtaactacca ctcgatctca
      601 tcttcagaac gctctaattt acgctgtgag acgttttgac gataaaataa ggttttctcc
      661 tgctctaaca cttctaattc ctcagaaagc cccatatcct tttctgcaag accaaaacgg
      721 atagacaaac gagtgatcga tttttgaaat gcgtttttta atcgctctaa acgctggcgt
      781 aaagagcgat tttcttcttt cagcttttta ttttccactt gtaaatctgc taattgtttt
      841 ctaccagaca aatgacgctc ttcttccaat gtagctacat actcatttaa ttctgtattt
      901 tgctcaagca tctgtaaata ttgctcactc atgttatcag catttctctt ggcatttgtt
      961 gctctcgtag ctcgttgata caaatcaaca aaatcattcc cagataaatg atacgaacca
     1021 tcaggaagcc tcttgaaact ttctaaagga accatatctt tttggaaaat gaccgccatc
     1081 tcttttttta cattcagcat atttttcttc tcttcttcaa tcttcgctct ctcagaagcc
     1141 atcagagcct tatcacgctc gttttcttct ctttgcctag aaatatacct caactcatca
     1201 gaaacgctct cacgctcttt tacagccgtt tggcgcaatt cagaaatttc actcatgatt
     1261 tctttgtatt cgtgaatatc tttaattttg ttggtaccga caatatccct tttccaaccg
     1321 taatcattca ttaatcgttc tatagagtca acttcttgat ttctaaaatt attgaaaagc
     1381 actcgaccat tatctttatc agcgtgtatc ccttgttgaa ccagtgcttt attaaagctc
     1441 ggctgctttt gaacaccccg tttataacct tcagcaaccg gcacaatatt caagtgcagg
     1501 tgcggactta tttcgtcatt atgaataaca gcattataaa cagccaaatt agggtttctc
     1561 tcttgaaact ccttgtaata ctgaaccaaa attttattgg caatcactcg attttgttca
     1621 gcttgttgtt tcgatatgcc agtagaagaa ccatctgctc gatttgtctg gaaatcatct
     1681 aaaatgccta tttgcacaat aaattctcgt tgaggttcca actttttatc cttgcgcact
     1741 ttcgctaaat agtcctcgat ttttctatcg gctctttttt gtttcgcatt atatttttct
     1801 accacttcgc caaacaattc ctcatattta tctgctagat tcgtctgctc taaataaata
     1861 ttttcatccg atttcgtgaa gtcaatatgc ttatgccaat cattggtttt ctggcgatca
     1921 gttaactcac gattattatg agaaatactt gtattcttcg ttcctttttt aaatgaaata
     1981 ctaaacccca agttaaaacc ctccttcgcg ctgtcgctaa taaaaagatt gtaaaccttt
     2041 ctgttactct ttgtcaaaga gtaacatttt cctaattgtg acatggctgt cacaattcca
     2101 aatgctctcc gagggtattc gctgtcacaa gtgacaacga aaaaaggagc cgaaagcgtg
     2161 tcacgctctc aactcctttt acgctttatt ttgcttcacg caaaataatc gcttatcgta
     2221 aattcacgtc attaaatatt gacgtaaatt tactctcact catctgactt ttcttattcg
     2281 gtatcactcc gaataagccg tcagattcat cgggataatc tcgttcgctt ggcgctgatt
     2341 tttcatcagc aagccactca ttatccactg atttttcgtg ttgctgatcg tcagaaatcg
     2401 acgatagcca ccgctcaaaa tcagcaaaag tttcaaaccc aaaacgcttg cgaagagcct
     2461 tgcccattgc agcattttct ttctcaatca agcgtttttt ctgttggcga agtttcgctt
     2521 gttctttttt taatttttct tcttgcgctt taatatctgc taaagtcatt cccataaact
     2581 acatctcctt ttctttggaa ttaataacgg aaatttggtt taactgcata acatgactca
     2641 ttaatttcaa ccaaaattct ttttctgatt catcagttgt ttgcgctaaa tgcttatgaa
     2701 caaattcatt agtcaaaact gaagattttg ctagatcagc attgataacc gagttgagaa
     2761 catccatcat tcgttcaagc atttcactat aattatcttc atccggcaaa ttcaaataat
     2821 cagctaaccg ttcatacatc aattcaattc tatcgactgt ttcttctctc aaatcaattt
     2881 tatccatgtc acacctcact tccagaattg ccaccatttt cgatttttta attcttgatt
     2941 ttctagtaac aactgatcgt ttttttgttg ctgaagcaaa gtcaattttt gttggtcacg
     3001 aatcatttcc gttaattcag aaatatgcct atctttttcc gctaattgtt cacgcaaaaa
     3061 ttcattttct ttttctaacg ggaaatcatt gcctgcatca ttgaccggta aatcaccagt
     3121 caattgacca ccttttgacc gataaattga ccgcacaaca ccaatcaatt ccttgtctaa
     3181 catgataacc ccattatctt tataccgagc aaggtcataa cctgcattga ccagttgatt
     3241 gcgcactgta tttttagata tacccaactc atcagcaagg ttttttatgg tcattttttc
     3301 tggcaaatca gtcattatct tctccgttac tttcagctac cccataaaca tatttaacag
     3361 catgcttagg gataatgatt ggggaatcaa ttttattatt tttcaaaatc aactcatact
     3421 gcgttacatc aagtaactta ccggacattt ttttgctatc caaaaaaaca acgacaacgt
     3481 tatcacctgt taaattcacc aaccaatttt tcttaccaaa atgcttttgt ttttctgcca
     3541 ttcttattcc tcctccgtta catctaaccc ttgaagccaa gtgtctaatt cttttttcaa
     3601 aacatctgct gattccgaaa atgtttctcg taaatgattc gtcaactcat cattttgact
     3661 ttctcttttc aaaatatctc tcaatttgta tttcattcta cgctctaaaa acccccacat
     3721 ttcagggtta gcaatcagtc caaaaatcat caccttatca tttgttttta catcaagtgg
     3781 tagaaaatga gggctagcaa aactatttaa actttcatga accattgcat gccaatctgt
     3841 tgctttccca cgccttaact gcaattccag tcgccaccaa gtttctattt ctggtggcac
     3901 aattttacgc ttggtttcct gttccaattt tttgttgtat agccgaatct ggcgttcgct
     3961 ggcacgactg ccccaataag ctgtttccag cttcccagac cgcccataaa ttggcttgaa
     4021 agaaacagga tcaaccactc gatattgtgt aataaactca tcaggcacat cgataatatc
     4081 gcaagcaata tccgcacgtg agaaatgtgg ttctagaaat aaatcatgaa tgaaattttt
     4141 cattgaacta gctaaaaaat gaccaatctt attcgggtta aaatcaatcc gccctttgcc
     4201 atcttgccat ttcaaaatct caatataagc aatattttct tcgtattggt ctaataattg
     4261 ccagccgtta ttcccaaccg cttttaaata gccattgcct tcattcaccc gcattaattg
     4321 ctcaaaatct aaaatcaaca catcattttg agtgtgataa taaatatttt ccttcagttt
     4381 tccaacgata gtaattcggt caattgacca ctgcattttt aagggggtgt acatattttg
     4441 tacatttaac cgcctgttag atagggctgt taaattttcc cactcattta cgattatgcc
     4501 atttttactc aaattttgtt cctcctcaaa gaaaaagaga cttctggaaa gaattttcaa
     4561 aagttcacgt aactcttaaa aattcattac caaaaaatct ctagtcttga agaaatctaa
     4621 aaatattcgt ataatgatta agaagttacg gagctagtgc gattagtttc cgtaaaggtc
     4681 gagaacgttg gtagcgttgc tcgactattt ttttactctt ttttcagttc atttgtttgc
     4741 taactcaata atagcacttc tctttagcta tgcaaatatc caaaaaataa aaccgcaccc
     4801 tttgaaaagg gcgcccccaa acttcgctaa cgctaggttg gccaaaaaat ggactggcca
     4861 ttttttagca aatgtcagct taataggcta gtggtttagc cgtcaactcc ttatcctcgg
     4921 cacaaaccgc ctccggtacc gttgactgca catcgtaact accactcgat ctcatcttca
     4981 gaacgctcta atttacgctg tgagacgttt tgacgataaa ataaggtttt ctcctgctct
     5041 aacacttcta attcctcaga aagccccata tccttttctg caagaccaaa acggatagac
     5101 aaacgagtga tcgatttttg aaatgcgttt tttaatcgct ctaaacgctg gcgtaaagag
     5161 cgattttctt ctttcagctt tttattttcc acttgtaaat ctgctaattg ttttctacca
     5221 gacaaatgac gctcttcttc caatgtagct acatactcat ttaattctgt attttgctca
     5281 agcatctgta aatattgctc actcatgtta tcagcatttc tcttggcatt tgttgctctc
     5341 gtagctcgtt gatacaaatc aacaaaatca ttcccagata aatgatacga accatcagga
     5401 agcctcttga aactttctaa aggaaccata tctttttgga aaatgaccgc catctctttt
     5461 tttacattca gcatattttt cttctcttct tcaatcttcg ctctctcaga agccatcaga
     5521 gccttatcac gctcgttttc ttctctttgc ctagaaatat acctcaactc atcagaaacg
     5581 ctctcacgct cttttacagc cgtttggcgc aattcagaaa tttcactcat gatttctttg
     5641 tattcgtgaa tatctttaat tttgttggta ccgacaatat cccttttcca accgtaatca
     5701 ttcattaatc gttctataga gtcaacttct tgatttctaa aattattgaa aagcactcga
     5761 ccattatctt tatcagcgtg tatcccttgt tgaaccagtg ctttattaaa gctcggctgc
     5821 ttttgaacac cccgtttata accttcagca accggcacaa tattcaagtg caggtgcgga
     5881 cttatttcgt cattatgaat aacagcatta taaacagcca aattagggtt tctctcttga
     5941 aactccttgt aatactgaac caaaatttta ttggcaatca ctcgattttg ttcagcttgt
     6001 tgtttcgata tgccagtaga agaaccatct gctcgatttg tctggaaatc atctaaaatg
     6061 cctatttgca caataaattc tcgttgaggt tccaactttt tatccttgcg cactttcgct
     6121 aaatagtcct cgatttttct atcggctctt ttttgtttcg cattatattt ttctaccact
     6181 tcgccaaaca attcctcata tttatctgct agattcgtct gctctaaata aatattttca
     6241 tccgatttcg tgaagtcaat atgcttatgc caatcattgg ttttctggcg atcagttaac
     6301 tcacgattat tatgagaaat acttgtattc ttcgttcctt ttttaaatga aatactaaac
     6361 cccaagttaa aaccctcctt cgcgctgtcg ctaataaaaa gattgtaaac ctttctgtta
     6421 ctctttgtca aagagtaaca ttttcctaat tgtgacatgg ctgtcacaat tccaaatgct
     6481 ctccgagggt attcgctgtc acaagtgaca acgaaaaaag gagccgaaag cgtgtcacgc
     6541 tctcaactcc ttttacgctt tattttgctt cacgcaaaat aatcgcttat cgtaaattca
     6601 cgtcattaaa tattgacgta aatttactct cactcatctg acttttctta ttcggtatca
     6661 ctccgaataa gccgtcagat tcatcgggat aatctcgttc gcttggcgct gatttttcat
     6721 cagcaagcca ctcattatcc actgattttt cgtgttgctg atcgtcagaa atcgacgata
     6781 gccaccgctc aaaatcagca aaagtttcaa acccaaaacg cttgcgaaga gccttgccca
     6841 ttgcagcatt ttctttctca atcaagcgtt ttttctgttg gcgaagtttc gcttgttctt
     6901 tttttaattt ttcttcttgc gctttaatat ctgctaaagt cattcccata aactacatct
     6961 ccttttcttt ggaattaata acggaaattt ggtttaactg cataacatga ctcattaatt
     7021 tcaaccaaaa ttctttttct gattcatcag ttgtttgcgc taaatgctta tgaacaaatt
     7081 cattagtcaa aactgaagat tttgctagat cagcattgat aaccgagttg agaacatcca
     7141 tcattcgttc aagcatttca ctataattat cttcatccgg caaattcaaa taatcagcta
     7201 accgttcata catcaattca attctatcga ctgtttcttc tctcaaatca attttatcca
     7261 tgtcacacct cacttccaga attgccacca ttttcgattt tttaattctt gattttctag
     7321 taacaactga tcgttttttt gttgctgaag caaagtcaat ttttgttggt cacgaatcat
     7381 ttccgttaat tcagaaatat gcctatcttt ttccgctaat tgttcacgca aaaattcatt
     7441 ttctttttct aacgggaaat cattgcctgc atcattgacc ggtaaatcac cagtcaattg
     7501 accacctttt gaccgataaa ttgaccgcac aacaccaatc aattccttgt ctaacatgat
     7561 aaccccatta tctttatacc gagcaaggtc ataacctgca ttgaccagtt gattgcgcac
     7621 tgtattttta gatataccca actcatcagc aaggtttttt atggtcattt tttctggcaa
     7681 atcagtcatt atcttctccg ttactttcag ctaccccata aacatattta acagcatgct
     7741 tagggataat gattggggaa tcaattttat tatttttcaa aatcaactca tactgcgtta
     7801 catcaagtaa cttaccggac atttttttgc tatccaaaaa aacaacgaca acgttatcac
     7861 ctgttaaatt caccaaccaa tttttcttac caaaatgctt ttgtttttct gccattctta
     7921 ttcctcctcc gttacatcta acccttgaag ccaagtgtct aattcttttt tcaaaacatc
     7981 tgctgattcc gaaaatgttt ctcgtaaatg attcgtcaac tcatcatttt gactttctct
     8041 tttcaaaata tctctcaatt tgtatttcat tctacgctct aaaaaccccc acatttcagg
     8101 gttagcaatc agtccaaaaa tcatcacctt atcatttgtt tttacatcaa gtggtagaaa
     8161 atgagggcta gcaaaactat ttaaactttc atgaaccatt gcatgccaat ctgttgcttt
     8221 cccacgcctt aactgcaatt ccagtcgcca ccaagtttct atttctggtg gcacaatttt
     8281 acgcttggtt tcctgttcca attttttgtt gtatagccga atctggcgtt cgctggcacg
     8341 actgccccaa taagctgttt ccagcttccc agaccgccca taaattggct tgaaagaaac
     8401 aggatcaacc actcgatatt gtgtaataaa ctcatcaggc acatcgataa tatcgcaagc
     8461 aatatccgca cgtgagaaat gtggttctag aaataaatca tgaatgaaat ttttcattga
     8521 actagctaaa aaatgaccaa tcttattcgg gttaaaatca atccgccctt tgccatcttg
     8581 ccatttcaaa atctcaatat aagcaatatt ttcttcgtat tggtctaata attgccagcc
     8641 gttattccca accgctttta aatagccatt gccttcattc acccgcatta attgctcaaa
     8701 atctaaaatc aacacatcat tttgagtgtg ataataaata ttttcctt
//
