LOCUS       NZ_CP040703            10664 bp    DNA     circular CON 25-JUL-2023
DEFINITION  Enterococcus faecium strain HOU503 plasmid p3, complete sequence.
ACCESSION   NZ_CP040703
VERSION     NZ_CP040703.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN11848047
            Assembly: GCF_005952885.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10664)
  AUTHORS   Shamoo,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAY-2019) Biosciences, Rice University, 6100 Main St.,
            Houston, TX 77005, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. Mar-2017
            Assembly Name                     :: HOU503F_del_liaR
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 10.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/25/2023 04:46:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,942
            CDSs (total)                      :: 2,851
            Genes (coding)                    :: 2,745
            CDSs (with protein)               :: 2,745
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 106
            CDSs (without protein)            :: 106
            Pseudo Genes (ambiguous residues) :: 0 of 106
            Pseudo Genes (frameshifted)       :: 38 of 106
            Pseudo Genes (incomplete)         :: 59 of 106
            Pseudo Genes (internal stop)      :: 27 of 106
            Pseudo Genes (multiple problems)  :: 15 of 106
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP040703.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="HOU503"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="p3"
                     /geo_loc_name="USA: Houston"
                     /collection_date="2015"
                     /note="liaR deleted isolate"
     gene            455..541
                     /locus_tag="FGF98_RS00005"
                     /old_locus_tag="FGF98_00005"
     CDS             455..541
                     /locus_tag="FGF98_RS00005"
                     /old_locus_tag="FGF98_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_110300541.1"
                     /translation="MFELFICPLLVGVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(662..1351)
                     /locus_tag="FGF98_RS00010"
                     /old_locus_tag="FGF98_00010"
     CDS             complement(662..1351)
                     /locus_tag="FGF98_RS00010"
                     /old_locus_tag="FGF98_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298772.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFLG
                     CIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKIEEA
                     CSDLFGDTNPWFASEEEKKEKQQSVTYERYEILLQQATNMQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(1591..2505)
                     /locus_tag="FGF98_RS00020"
                     /old_locus_tag="FGF98_00020"
     CDS             complement(1591..2505)
                     /locus_tag="FGF98_RS00020"
                     /old_locus_tag="FGF98_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002340563.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQTAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2487..2867)
                     /gene="mobC"
                     /locus_tag="FGF98_RS00025"
                     /old_locus_tag="FGF98_00025"
     CDS             complement(2487..2867)
                     /gene="mobC"
                     /locus_tag="FGF98_RS00025"
                     /old_locus_tag="FGF98_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_002317440.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASGDA
                     QRLSENLKIMQKELQKIWQQLR"
                     /name="mobC"
     gene            complement(3309..3446)
                     /locus_tag="FGF98_RS15585"
     CDS             complement(3309..3446)
                     /locus_tag="FGF98_RS15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002303562.1"
                     /translation="MILLKKEYEKFYDRLIYSLQLPKKLNRQLQKTYETKSTEEWESYC
                     "
                     /name="hypothetical protein"
     gene            complement(3560..4180)
                     /locus_tag="FGF98_RS00030"
                     /old_locus_tag="FGF98_00030"
     CDS             complement(3560..4180)
                     /locus_tag="FGF98_RS00030"
                     /old_locus_tag="FGF98_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_002303561.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTLL
                     NNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAKKF
                     NSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEPTGN
                     LDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
                     /name="ABC transporter ATP-binding protein"
     gene            complement(4177..5217)
                     /locus_tag="FGF98_RS00035"
                     /old_locus_tag="FGF98_00035"
     CDS             complement(4177..5217)
                     /locus_tag="FGF98_RS00035"
                     /old_locus_tag="FGF98_00035"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_080341602.1"
                     /translation="MKKASKFVYKIQKNYKTFIIAPYNYATIQENNKEFFIGQKVYPNF
                     EDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTTYILVPKKYKEYAKMIE
                     KNYLEDIQFRLEDSPPQAPYKNPNLKNLKIKLIFVNNNQKYFSFNTLYGKAKDNYTITD
                     PIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQINPYIKEFNLENIINFSY
                     SVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIFVSFTINSKKIALKKIFGYS
                     PISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIFMIFLINLITSIIIYKKNSFKY
                     FKENIL"
                     /name="hypothetical protein"
     gene            complement(5282..6460)
                     /locus_tag="FGF98_RS00040"
                     /old_locus_tag="FGF98_00040"
     CDS             complement(5282..6460)
                     /locus_tag="FGF98_RS00040"
                     /old_locus_tag="FGF98_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="WP_000997695.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDYE
                     KYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLETTI
                     IHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAIFMD
                     ATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRGVQEV
                     LLFITDGLKGMKDTIHQIYPKAKYQHCCIHVSRNIAHKVRVKDRKEICDDFKAVYQANS
                     KEEANTFLSGMIEKWKKNYPKVTQSLIENQDLLTFYDFPPSIRRTIYSTNLIESFNKQI
                     KRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLVSMFTE"
                     /name="IS256-like element ISEf1 family transposase"
     gene            complement(6558..7649)
                     /locus_tag="FGF98_RS00045"
                     /old_locus_tag="FGF98_00045"
     CDS             complement(6558..7649)
                     /locus_tag="FGF98_RS00045"
                     /old_locus_tag="FGF98_00045"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_254698536.1"
                     /translation="MLKKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQY
                     KDDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILARN
                     DSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLKKYG
                     TPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNSFDTI
                     KDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIVTLSIY
                     HLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINTHNEAENNSLKYWERTT
                     NLYKTNITNQLNRNNTVEENKRV"
                     /name="hypothetical protein"
     gene            complement(7703..8077)
                     /locus_tag="FGF98_RS00050"
                     /old_locus_tag="FGF98_00050"
     CDS             complement(7703..8077)
                     /locus_tag="FGF98_RS00050"
                     /old_locus_tag="FGF98_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_002305242.1"
                     /translation="MKKFLCLSALMGVLLTSGGIASATEALNLDVPEDHTAIYGGGMEA
                     TEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKAGV
                     RAYATCNATLTGNTGWWNVY"
                     /name="lactococcin 972 family bacteriocin"
     gene            8210..8671
                     /locus_tag="FGF98_RS00055"
                     /old_locus_tag="FGF98_00055"
     CDS             8210..8671
                     /locus_tag="FGF98_RS00055"
                     /old_locus_tag="FGF98_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_002305240.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPDI
                     ETLKKICEFYSTNINSILNEDNSKNKKNNLLVLTAIIFNIFVTILGLFELIQMNFFSFT
                     HGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTMFFPLVILIILLS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(8983..9153)
                     /locus_tag="FGF98_RS15590"
     CDS             complement(8983..9153)
                     /locus_tag="FGF98_RS15590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002292472.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(9169..9708)
                     /locus_tag="FGF98_RS00060"
                     /old_locus_tag="FGF98_00060"
     CDS             complement(9169..9708)
                     /locus_tag="FGF98_RS00060"
                     /old_locus_tag="FGF98_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002289340.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKVV
                     LRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLREQL
                     SLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEETESI
                     EKHTKKKKQKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            complement(9736..10473)
                     /locus_tag="FGF98_RS00065"
                     /old_locus_tag="FGF98_00065"
     CDS             complement(9736..10473)
                     /locus_tag="FGF98_RS00065"
                     /old_locus_tag="FGF98_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002305239.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEIF
                     TFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDKVN
                     ETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKGHWF
                     VKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAYKGNK
                     IGSLQIYFEENIPSITLDNWLEK"
                     /name="replication initiation protein"
     BGC             2703..10664
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(9737..10473)
                     /name="rep_cluster_1742"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1595..2505)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.342"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 taatccctaa cagttattat taacagtata tctaacagta tatctaacag tatatcttaa
       61 gagcacattg aatttcccgc ctaaaaaact cctacttttt cctccaaaaa ggttcgcgaa
      121 tttttctaaa ctggaaaact tgctccctac ggtcgctaaa aaggtgtgat cgctatgctc
      181 tcatattata ctgcgcagtc gcctccggct cctttgctaa aaccgttgtt caaagaggag
      241 gtgtgagact ctgaaagctt gtctaagccg ttctaagaca attcttgatt aaatttccct
      301 taatctagtc gagaagcgcc ttacaggcgg tcagggcaag ccctaacaac cctaaaacaa
      361 gtgatactta aaaacaccac acaaatttaa ttttttaaga tataataact tgtcgcctat
      421 ccgacagtct tgcgactgga aggaggtgta tctaatgttt gaactgttca tttgccccct
      481 tcttgtagga gtttgtgtct ctttattcag ttattggttg aataaccgaa acaagaagta
      541 aaatcaataa aggcgacatc aacctgaaaa aaagccccag agaatccgac ctctctgggg
      601 ctttcgtgta tctaatgttt gatcatttgc acattcagtt taacatgatt ttaaatcaaa
      661 ttcaattttg ttgttgctga tctaaccatt ccttgacact ttttagatag tttaaggcgg
      721 tttctttttt tgtcggattt tgcatgttag ttgcttgttg aagtaagatt tcatagcgtt
      781 catatgtgac agattgttgt ttttcttttt tttcttcttc actggcaaac caagggtttg
      841 tgtctccaaa tagatcacta caggcttctt ctatctttgt atcttttaca tattgcacgt
      901 cttttttggt tcgctcatag ttgtcttgaa cctctttttg ctgtttttct ggcaagtttt
      961 gataaagacg agtgagtttt tccatgtttt tatccgtcaa acgtttttct tgattcacaa
     1021 aaatatcgtt catttcctca taacaagtgg taaactcttc cattttttgt gctaatgctt
     1081 gctctttttt ctttttatca cgcgctgatt catacttttg agtgacattt cctaataaaa
     1141 tacttctaga aacgcgctta tttccctcta aaacgccgaa taagccgctt gaaacgatta
     1201 tcaaggaaaa tatacagcca agaaagaaaa atcgcttaaa tcgaatttta gagcctttta
     1261 atgaatatct tgtttgaaaa attttataga tgagccaaat acttcctgaa atactaacaa
     1321 aaatgagtag atagggtaaa taaaatgcca ttttagcctc cccatactaa ccaaaatgcg
     1381 aacagtgtca ggactaagca acataagggg gcaagaaaat ccaatgatcg ttgaataagc
     1441 acctttttct gttccttatt tcgctggtct aatagctctt gatggagttt tttaacttgt
     1501 aaatcaatcg tagtttgtat cctttcttgc gtataaggaa cgagaaaagc acgttgtttc
     1561 tttatttcgt tttctatgtc ttttaacgtt ttagagcgat aattcttgtt ggtggtctgt
     1621 tcctctttca acgtgttgtt catggtttct tcgagccggt ctaatttccg gttgttcagg
     1681 cgtaagagtt cgttgttctc ttcgtacttc ttctgcagtt tcgcgaatgc tagctcgagc
     1741 tcttcgtagc tcgttggttc gctgttggtt ataggttcgt tctcgtctgt ccagctcatg
     1801 taaaatcgtc tccttttcgt agtcctttcc tagtctgttg gatcgtactt ttttcttttt
     1861 gtggatcaat tcgtaagtga aatttttccc gcgattgaag caatgaatcc ccaatgtttt
     1921 gagggaatta caaaattggt cataggttct ggtttttttg tttttcattg tttgatcgat
     1981 cgcaatccga atttcgtctt tccaaacagg ttcatttttt tcttttaatt ttatttcaga
     2041 catggttcgt ttttctggtt tctttcctcg ctcagtgatt ggtaagttat gtgcaagaca
     2101 aatctcgtca tttaggtcat aacatttgtt tagaaatgct tgaccatgag cttgaaattt
     2161 ttttccagtc tccagattaa cggagttaat gacaatgtga ttatgaatat ggtctttgtc
     2221 tgtatgtgtg taaatagctg tttggtgatc gggggctatt ttttttgcat actctgcccc
     2281 tagttgatta gctttttcag cggtaatttc tcctggtcga aaagattgaa tcaataagtg
     2341 tgcctgtaca cggtcgtctt ttccatacag catgcgaatt tgtttcattt cacttttggc
     2401 ataatcgacg tcacagttta ttcctgtttt aatggttgct cgtggttctg cgtaattaat
     2461 tgcacgacta caagaagtgg ttcggcttac cttaattgtt gccatatctt ttgtagctcc
     2521 ttttgcatga tttttaggtt ttcggataaa cgttgagcgt caccagaagc aacgtcatgt
     2581 tggtgtgtat tgcaccactt agcaatttga ttgatattcc ctccagcttt ggctaaatcg
     2641 cgcgcgattg cctgggcaat gtctggcgcc actttcggtg tcaccagacg cgcgttttgt
     2701 gccttggctt tcacgaaagc cggcacactc atttgcaaag cttcagccga ttgcttcagc
     2761 tttgcaaatt cggattcgct cactcgaaaa ctgatttgtt ttggctcgga tcgataggta
     2821 tattttttag aagaattgct agccaaattt tgattttgtt cgctcataag tcccaccctt
     2881 tcagtacgtc ctacggacgt atcgttcgca ataccattgt acctaaaaag gtacaatgag
     2941 taaagctcac taaaagggtt tggcaagctc tatatttgct agccaaatac ctacgtattt
     3001 agtgcaaata accgcttgtc aaaggggcgc aagctccccc ttgaatcccc ctcaaaagcc
     3061 acgctccaca atacaaaaaa agctagcaaa tgctagccac tttttgtaaa gtttcgctta
     3121 tttcaccgaa gatgaaattg ttactttgca acaaaaaaca taaatgcttt tttgtgtaaa
     3181 ctactaacta tttagctaaa actgatagtt ttattaaaca ttcagcaata tttttagcca
     3241 attttttatc agactttcgg cgagtcatta tgataatatg tccacttttt gttacttcta
     3301 ccaaaaatct agcaataact ctcccattct tcagttgatt tagtttcata agtcttttgt
     3361 aattgacggt ttaatttttt tggcaattgc aaagaataaa taagtctatc ataaaatttt
     3421 tcatactctt ttttcaataa aatcactagg gtgcctcctt tttattaagt gattatttaa
     3481 attttgggat aggagagatg gaatataaaa taaatacccg tggtgctagt tttaaatcag
     3541 ctagcatcac gggtaaaaat tataattcta taatattagt tccatagttt agaacattta
     3601 agtcatgtgt tactagtaca acggcttttt tttctttttc tgttaaattt ttcagaaagt
     3661 tcattataaa agttgagttt tctttatcta aatttcctgt aggttcatct gcaaataata
     3721 tatcaaatgg ttttaacata tttctaatta atgctattct ttgttgttct ccaccgctta
     3781 agagaaagat tttttctttt aagatatctt ttggcaaaga aaaatctgtt aataaattat
     3841 aaatatgatc attggaaata ttactgttaa acttttttgc taattcgaaa ttgtattgta
     3901 ctgtttgatt ttcaagaaga gcatagtttt gaaataaata gctaatatga tttcttcgta
     3961 gacgttgtat atcatgttta gaaactttct tttcaaaata gaataagtta ccggtatatt
     4021 gtttatctat taggcttaaa ttatttaaca gagtagtttt tccactacca ctttttcctg
     4081 ttactataat aaaatctttt tctgatattg aaaagttcca attttgtttg atcttttttt
     4141 tattaattga cagggtgaaa ttctcaactg ataatatcat aaaatattct ccttaaaata
     4201 tttaaaacta ttttttttat aaataataat agaagtaatt agatttatta aaaagatcat
     4261 aaaaattata aaatacaagt aagtgttttt tgaaaatatc aattgaaaac acatagtagc
     4321 aaataactct attgtaaaag ggagtaaaca taaatctaaa acttgactta ttggtgaata
     4381 tccaaatatt ttttttaaag ctattttttt tgaatttata gtaaaactaa caaaaatcat
     4441 ttgaaaatta attattataa ataatcctaa tattaaaaga tattgtagat atgcatttaa
     4501 tttatttact tttttttgag ataataattc accagtttct tgaaaaacag aataagaaaa
     4561 atttatgata ttttctaggt taaattcttt tatatacgga ttaatttgat taaaaaagtt
     4621 ttggtttgaa gtatgtttct gatcaaaaaa taatccacca tcactagtca atatatttcc
     4681 ccaaaataat gattctgtta tttctggatt aataactcta actatgggat cagttattgt
     4741 atagttatct tttgcctttc catataaggt attaaaacta aaatactttt ggttattatt
     4801 aacaaatatt agttttattt ttaaattttt caaattagga tttttataag gagcttgagg
     4861 aggagagtcc tcaagtctaa actgtatgtc ttctaaataa tttttttcaa tcatttttgc
     4921 atattcttta tattttttag gtactagaat gtaagtagtt agtggatcag agtttattaa
     4981 atttaagtca gtagattctt caaaagaaat aggattgtaa tattttagat agtttaaatc
     5041 aacacatatt cctgcaccag caggttgact aacatagtct tcaaaatttg gataaacttt
     5101 ttgtccaata aaaaattctt tattattctc ttggatagtt gcgtaattgt atggcgcgat
     5161 aataaaagtt ttataattct tctgtatttt ataaacaaat ttactagctt ttttcaagta
     5221 gttattttcg ggagcgtcaa taattttgtg taaataaatt gtcctcctgc aaaataatta
     5281 gttactcagt aaacattgaa actaatgtat cggttacctg ttgaaaacct ttatggcttc
     5341 tgtttagaaa tttttgattg tatgtatcaa aaatgctgac tagaaagcgt tctagtgatt
     5401 cttcattttg aaactgctct tttctacggc tgtatctttt aatttgctta ttgaaagact
     5461 cgattagatt ggttgagtaa atggttctac gaatgctagg tggaaaatca taaaaagtta
     5521 ataagtcttg gttttctatg agtgactgcg tcactttagg atagtttttc ttccatttct
     5581 caatcatgcc ggataagaag gtattcgctt cttcttttga gttagcttga taaacagcct
     5641 taaagtcatc acagatttct tttcggtctt tgacacgtac tttatgagcg atgttacgag
     5701 atacatggat acaacaatgc tgatattttg ctttaggata aatttgatgg atagtatctt
     5761 tcatgccttt taagccgtcc gtaataaaaa gcaagacttc ttgaactcct ctggagttaa
     5821 tatcctgtag cagctcattc caaacgtatg ttgattcagt tggagcaatc gcataactca
     5881 gtacttcttt agtgccgtct tctcgtatac caatggcaat ataaatcgct tctttggata
     5941 cggtttgacg ttttagtgga atgtaagtag cgtccataaa aatagcgaca tacttatcat
     6001 ttaaggctct ggatttaaag gcatttactt cttcagtcag aactttagtc atgttggaca
     6061 tggtttgtgg agtatagtga tgaccgtaca ttttttcgat caaatcagca atttcagaca
     6121 tcgtaacacc tttttcgaat aaatggataa tagtggtttc caatgtatcg tttgttcttt
     6181 tgtaggctgg taaagtttgt tgtttaaact caccattacg atctctaggt atttccaatg
     6241 ttaattcacc atattcggtt ttgattgatc gaaagtaaga accgtttctc gaattacctg
     6301 aattaaaacc agtgcgatca tatttttcgt aatctaaaaa agccgttaat tcagtccgta
     6361 ggagtgtgtt tatcgctttt tctaagtgcg aacggaataa ttcatttaaa tcgcctttag
     6421 tgactagagt ttgcacaatt tctgtagtaa aatcattcat agggaagtcc tcttttctgt
     6481 gaattggttg tcgttaactt tattctacag aagggacttc cttttttgta tggatttttt
     6541 catttacaca aaatatttta tactctctta ttttcttcta cggtgttgtt tctatttaat
     6601 tgattagtaa tatttgtttt gtataaatta gtagttcttt cccaatattt aagagaatta
     6661 ttttcggctt cattatgggt atttattact tttgtagaag ctattaacag aaggatcatg
     6721 cttaagaata gacagatatt taatatacaa agatttaact taagtggtcg tgaatttttt
     6781 agataatttt ttatactaga taaatgatag atacttagag taactatgat aaataaaaaa
     6841 ctaattataa aatgaaaaat actacataga agaaatgata ggaaaaaatt ttcgttactg
     6901 taatatatgc tcaaaaaaat agataaaatg aaagttataa tcaaagaaca aaataagagg
     6961 tttaactctt taatagaatc ttttatagta tcaaaagagt tatagcctaa catttttttt
     7021 agagtgattt ctttcatttt tgaaaaagca aatataattg tagtaattac aataaatata
     7081 ctaagatagc agataacaag aacaatatta gtattaattt gaaaagtatc tgattgattg
     7141 atattggaaa tagtaggtgt tccatatttt ttcagtacag gaattaattt gtttgagttt
     7201 ccttgtacat ataatatttc acttaatcca atattttcca catgagaaaa aggataaata
     7261 tttatattta aaaaattatt tggaagtttt aattggtact tagaattttt gttattagta
     7321 taatggactt tagaatcgtt tcttgctaat atattttttt tagaaaattt ccagtgtttg
     7381 ttagcgttag ggtttgtagc aagaatagtt agttggttaa cagcagtaaa actatattga
     7441 gaaatattaa tattttcttg tgtagatatt tcatacaaag cgtttatata actgttaaag
     7501 tccatatcat ctttatattg catatgaatt tcagcggtat ttttatatag cgcttcagaa
     7561 gtctttatac catttataac attagtagaa aaaaatagta ttgtaatgca gacaaaaagt
     7621 gataaaacag cgtttatttt ttttaacata ttttgaattt cctttcaaat gtaaataaaa
     7681 aagagagttt aaactctctt ttttaataaa cattccacca accagtattt ccagtaagtg
     7741 tggcattgca tgttgcataa gctcttactc cagctttctg aactgatcgt acttgaccgc
     7801 ctcgtccatt tttagctgtt gcactatgtt tagctctagc atggtagtaa tttgcataaa
     7861 ctttaaatcc attaacccca cagttgaaat ccccgccgcc agcagcatat ctcaatttac
     7921 tagaagaaaa tccttcttct gtagcttcca ttcctcctcc gtaaatggcg gtatggtctt
     7981 cgggtacatc tagatttaaa gcttctgtgg cactagctat tcctccacta gttaataaga
     8041 cacccattaa agcagaaaga cataaaaatt ttttcataac ttatttctcc tatctttttt
     8101 actagaatta ttttatttta gtgtgtttta taaaataaag aacttttttg gcgcgttagt
     8161 atttttatga taaaatttta tgtaaataat cgtttacata ggagaaaaaa tggaggattt
     8221 aggatctaag ctaagagaat taagagtgaa aaaagggtat actcaagaat atgtggcaag
     8281 aaaatttaac gttacacgtc aaactatttc aaagtgggaa aataacaaaa caacaccaga
     8341 tatagagaca ctaaaaaaaa tttgtgaatt ttatagtact aacataaatt ctattttaaa
     8401 tgaagataat tcaaaaaata aaaaaaataa tttactggta ttgacagcta ttatttttaa
     8461 tatatttgtt actatattag gtttatttga attgattcaa atgaattttt ttagctttac
     8521 acatggaata tttacttggt ttgctctatc tggattaata gtaatagata tcttaaatta
     8581 tttttatatt tactattctt atttaaaaaa tgaaaaattt ttttatacaa tgtttttccc
     8641 cctagttatt ttgattattt tattaagcta atatgaagta gttagttatt taattgcata
     8701 gttgacataa tgtacactat gccaagtagc ccctctacca atggatagag acgctcactt
     8761 tgtgattttt aattggtcta gctaaaagca actttctaaa gcttgatttt tcgcggttat
     8821 ctatatctaa ttctttgtgc atttttatct gtcatttttt taagacttgt tattttttgt
     8881 aaaatatggt aaatttttgg tagcgcttca taagcgcgtg ctttttcaag aataaacact
     8941 cattattttt taactggctg ctcggaagag cagtcttttt tattatttat ccgtctcttt
     9001 tacaaacaat tcttcgttag gactgttgta ataatttgga ttcaatttca attcaccatg
     9061 tttattccct ttattgatta ctgacaataa catcggactg aagcctagaa ttcctagtaa
     9121 aatagaaccg aatagaagat tttttcttgt catgaatgtt ccctccagtt atttccaaag
     9181 ttgccaccat ttttgcttct tcttttttgt gtgtttttct atcgattctg tttcttcaga
     9241 aagaatagta gttgattcat cttctggagt ttcttccgta taagctaatt gtaactgttc
     9301 ttgcagtttt tcgatctgtt gatttgcctg tagggtgagc tgttgttgct ggtcgagtaa
     9361 tttttgcata ttagaattat tttgctcttt gagggaaagt tgttctcgta aaaattcatt
     9421 ttcttcacgc aacttatcta gcaactcgtt tacaatcacg tttttgcttg atatataaac
     9481 gtttttatcg ttttcttgtt catactcatg caacctatat agtaaccttt ggttacctaa
     9541 gttgctagca agttgcaata ctccagaacg taatacaacc ttataaccac gctcattttt
     9601 agctaataag gacgggtcta atttatcaac atgtttagag attgcttgcc tacttatttc
     9661 aaataattca gataattcct ttattgtaaa ggtttctttg ggttgcatga taattgcctc
     9721 ctaaattgtt aggtattatt tctctaacca attatctaag gtaattgaag gtatattttc
     9781 ttcaaaataa atttgcagag atcctatctt atttcctttg taggctttaa tttttttgat
     9841 ttcaaaagtt ttaaaaatag cagggtgagt tgaattaacg tttaaaaatt cttttttcgc
     9901 ttgggcaaaa atttgtttat caatatctct catgcgataa ctttctggta ttgctaatag
     9961 agctctaaaa tcttctattt ttacaaacca gtgacctttt ttagatcttc tgtctttatg
    10021 agccataagt tggcgatata actctttaac gtagctactc tttaagtttg tcatattttc
    10081 taattcgaaa cgagtaaaat tagttccgat tgaattaagt atatattcaa attcttcaga
    10141 aacctttatt aataatgttt cattcacttt atcgatttta aatttttgaa acagatttaa
    10201 ttgttcatat gttttttcgt cttcataaat aaaagacaaa gtaatgattt tttttgacat
    10261 ttttattaaa tcattagcaa aacggtcgcc acgtgcattt atattgtaat caattaattc
    10321 ttttatttgt ttaaatgtaa aaatttcttc ttttgtacct tttcttttca ttttagaaca
    10381 tattccccaa aataaatcca tatctatagg ggtaaatttt cttaaaggga cagaatttaa
    10441 tccattatta tatcgaacta tatcgttact caataaaaat cacctcaata aattatgtta
    10501 tcatagacat aagaggatgt aaaacacaat ttatccccca tagtgtgaca atttatcccc
    10561 catagtgtga caatttatcc cccatagtgt gacaatttat cccccatagt gtgacaattt
    10621 atcccccact acctatgtca atacttactc tcccataagg ttgc
//
