LOCUS       NZ_CP040633             2750 bp    DNA     circular CON 07-AUG-2023
DEFINITION  Bacteroides sp. PHL 2737 plasmid unnamed3, complete sequence.
ACCESSION   NZ_CP040633
VERSION     NZ_CP040633.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN08875372
            Assembly: GCF_004115325.2
KEYWORDS    RefSeq.
SOURCE      Bacteroides sp. PHL 2737
  ORGANISM  Bacteroides sp. PHL 2737
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Eshaghi,A., Graham,C., Zitterman,S.I., Kus,J.V., Soares,D. and
            Patel,S.N.
  TITLE     Molecular Characterization of a multi-drug resistant invasive
            Bacteroides spp. isolate
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Eshaghi,A., Graham,C., Zitterman,S.I., Kus,J.V., Soares,D. and
            Patel,S.N.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2019) Molecular Research, Public Health Ontario
            Laborato, 661 University Avenue, Toronto, ON M5G 1M1, Canada
REFERENCE   3  (bases 1 to 2750)
  AUTHORS   Eshaghi,A., Graham,C., Kus,J.V., Soares,D. and Patel,S.N.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2018) Molecular Research, Public Health Ontario
            Laborato, 661 University Avenue, Toronto, ON M5G 1M1, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1.0x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/06/2023 01:38:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,652
            CDSs (total)                      :: 4,559
            Genes (coding)                    :: 4,488
            CDSs (with protein)               :: 4,488
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 42 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP040633.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Bacteroides sp. PHL 2737"
                     /mol_type="genomic DNA"
                     /strain="PHL 2737"
                     /isolation_source="Abdominal ascites fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:2162637"
                     /plasmid="unnamed3"
                     /geo_loc_name="Canada: Ontario"
                     /lat_lon="43.6532 N 79.3832 W"
                     /collection_date="2018-01-01"
                     /collected_by="Public Health Ontario laboratory"
     gene            64..654
                     /locus_tag="DBK98_RS23015"
                     /old_locus_tag="DBK98_024345"
     CDS             64..654
                     /locus_tag="DBK98_RS23015"
                     /old_locus_tag="DBK98_024345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295513.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_004295513.1"
                     /translation="MENKKAVKLTDFQKNEENPFMKQAIEGIENHVVKKYKSNSGGDKR
                     AVVALADTETGEVFKTSFIRQIEVDEEQFTKLYLSNFAAFFDLSQAAIRVFGYFMTCMK
                     PKNDLIIFNRKKCLEYTKYKTDKAVYKGLAELVKAEIIARGPADNLWFINPLIVFNGDR
                     VTFAKTYVRKKTLAAQKKEEAEKRQLSLGFDEQ"
                     /name="replication/maintenance protein RepL"
     gene            1272..2375
                     /locus_tag="DBK98_RS23020"
                     /old_locus_tag="DBK98_024360"
     CDS             1272..2375
                     /locus_tag="DBK98_RS23020"
                     /old_locus_tag="DBK98_024360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_007219893.1"
                     /translation="MGATSIHVQAVKPGSEIHNFREKELDYVRPELSHLNESWVGDSIS
                     HRLESAKQRYLDTVGQKMQAKAAPIREGVIVIKQETTMQELQQFATVCKERFGIEAFQI
                     HIHKDEGYMNAKQWTPNLHAHVVFDWTQPNGKSVRLSRDDMAELQTIASETLGMERGVS
                     SDRKHLSAMQYKTECAKEQLQELSNDISSALDKHKDVQNQLLQLQKELRSIETKKNVQK
                     LISKASEKFYGLIGKTVNDREKDALKAKVKALEGENEQLSDRLGKAILEKEQNGTKAFK
                     AENDKEYYRQQMDNARTTSNRLRTENQELKTETKELKKELGKMKDLFNSEQLEALRHHF
                     PNISKAMEEGKDLLKQITRSRGFGMGM"
                     /name="plasmid recombination protein"
     Typing          32..644
                     /name="rep_cluster_1868"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.701"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1273..2372
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.275"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttgtttttat ggaaaataat agtttacttt gtggagaata atatttccca aaaacacatc
       61 aaaatggaaa ataaaaaagc agttaagtta accgattttc aaaagaacga agaaaatcct
      121 tttatgaaac aagctataga aggtattgaa aatcatgttg ttaaaaagta taagagtaat
      181 agtggtggcg ataagagagc cgtagtagct ttagccgaca ctgaaactgg agaagtgttt
      241 aagacttcgt ttatccgtca aatagaagta gatgaagaac aattcactaa attgtatctt
      301 tctaactttg ctgcattctt tgacctatca caagcagcta ttcgggtttt tggttacttt
      361 atgacctgca tgaaacccaa aaatgattta atcatcttca atagaaaaaa atgcctagaa
      421 tataccaaat acaaaacaga caaagccgtt tataaaggac ttgcagaact tgtaaaagct
      481 gaaatcatag cccgaggacc agccgataat ctttggttta ttaatcctct gatagtattc
      541 aatggtgacc gagtgacatt tgctaaaaca tacgttcgga aaaagacttt agctgcccaa
      601 aagaaagaag aagcagagaa acgacaatta tcacttggct ttgatgaaca gtaacactcc
      661 attgagtgaa gctgccgttt ggtcgctccc cctttggggg cgggggggga tagataaagt
      721 tcctctatgt aaagttataa tgggggatga aaggcaaggt tcgctaacct taccccgatt
      781 gtttatgctt cccagcattc aatctatccc cgtttatggg tttctctttc accctacaaa
      841 gataacctca tgggggaaaa atgtccaaga atatggggta aaaactatca agtcggtaga
      901 aaataagtat ctttgaagta catttttagt agaggtactg gtatgcctag acgaagcaga
      961 taggcaaaaa taaagccgct ggagatgtcg gatgtccgtc aattaactcc agcggcttgt
     1021 attttggtaa ctctcccgta tcggtcggct accgtttggt cagcaacaaa ggtagtactt
     1081 tattgtcgca aatccaaatg ttttcccctt tatttgtgca tttacagaac atttgctaac
     1141 ttcgcaccta tgcagtatgg tgatagcaca ctagcaccct aaagggtgcg tgtgtaaggg
     1201 gacaagcccc cttaaccccc tgtcagccgg ctgtcaccgg ctgttatcga ttgtcacaaa
     1261 aatgttaact tatgggagca acaagtattc atgtacaagc agtgaagccg gggagtgaga
     1321 ttcacaactt tagggaaaaa gagttggact atgttcgtcc cgaacttagt catttgaatg
     1381 aaagctgggt tggagatagc atctcccatc ggctggaaag tgcgaaacaa agatatctcg
     1441 atacggttgg gcagaagatg caggctaaag ccgcacccat acgagaggga gtaatagtaa
     1501 tcaaacaaga aaccaccatg caggaactcc agcagtttgc cacggtctgc aaagaacgtt
     1561 tcggtatcga agcatttcaa atccatatac acaaggacga aggatacatg aacgcaaagc
     1621 aatggacacc taaccttcat gcccatgtag ttttcgattg gacgcagccg aatgggaaaa
     1681 gtgtgcgttt atcgcgtgat gacatggcag aactccaaac catagcatct gaaacactgg
     1741 gcatggaacg tggcgtttct tctgaccgca aacacttatc ggctatgcag tacaagaccg
     1801 aatgtgcgaa agaacagctt caggaactat caaacgatat atccagtgca ttagacaaac
     1861 acaaggacgt acaaaaccag cttcttcagc ttcagaaaga actacgttcc attgaaacaa
     1921 agaagaacgt ccaaaaactc atttctaagg cttcagagaa gttttacggc ttgattggaa
     1981 aaacagtcaa cgatagggaa aaggatgcct taaaagccaa agtaaaggct ttagaggggg
     2041 aaaatgaaca gctatccgat agactgggaa aggctatact tgaaaaagag caaaacggca
     2101 caaaggcatt caaagccgag aatgacaagg agtattatag acaacaaatg gacaacgcaa
     2161 ggacaaccag taaccgatta aggacggaga accaagaact aaagaccgaa accaaggaat
     2221 tgaaaaaaga acttggtaag atgaaagact tgttcaattc cgaacaactg gaggctttaa
     2281 ggcatcattt cccaaacata tcaaaagcta tggaagaggg gaaagaccta ctcaagcaaa
     2341 tcaccagaag cagaggtttc ggtatgggta tgtaaccccc ccccgccccc aaagggggag
     2401 cgaccaaacg gcagcctctc tcaatggagt gttacgtcgt tcggattcgg aagaaatttt
     2461 gcagggttcg gaaaacggtt gtgtgcttat tctaaaaaaa gtttatggtg tggcgctcgt
     2521 cgggacgggt ggtcccgccc cttgccgctg ggcgctcccc gaccgatgat ttttaagtga
     2581 ctgattttgt gctgttttgg gggtatatta agaatagaag aaatagaata agttaagtac
     2641 ttgatacaca atatagggca ttttccatat tggaaattct cattttccaa tctagaaaat
     2701 actgatttcc taatataata ctaaacagga aaataatatt tcccttaata
//
