LOCUS       NZ_CP040632             5595 bp    DNA     circular CON 07-AUG-2023
DEFINITION  Bacteroides sp. PHL 2737 plasmid unnamed2, complete sequence.
ACCESSION   NZ_CP040632
VERSION     NZ_CP040632.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN08875372
            Assembly: GCF_004115325.2
KEYWORDS    RefSeq.
SOURCE      Bacteroides sp. PHL 2737
  ORGANISM  Bacteroides sp. PHL 2737
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 5595)
  AUTHORS   Eshaghi,A., Graham,C., Zitterman,S.I., Kus,J.V., Soares,D. and
            Patel,S.N.
  TITLE     Molecular Characterization of a multi-drug resistant invasive
            Bacteroides spp. isolate
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5595)
  AUTHORS   Eshaghi,A., Graham,C., Zitterman,S.I., Kus,J.V., Soares,D. and
            Patel,S.N.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2019) Molecular Research, Public Health Ontario
            Laborato, 661 University Avenue, Toronto, ON M5G 1M1, Canada
REFERENCE   3  (bases 1 to 5595)
  AUTHORS   Eshaghi,A., Graham,C., Kus,J.V., Soares,D. and Patel,S.N.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2018) Molecular Research, Public Health Ontario
            Laborato, 661 University Avenue, Toronto, ON M5G 1M1, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1.0x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/06/2023 01:38:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,652
            CDSs (total)                      :: 4,559
            Genes (coding)                    :: 4,488
            CDSs (with protein)               :: 4,488
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 27 of 71
            Pseudo Genes (incomplete)         :: 42 of 71
            Pseudo Genes (internal stop)      :: 17 of 71
            Pseudo Genes (multiple problems)  :: 13 of 71
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP040632.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5595
                     /organism="Bacteroides sp. PHL 2737"
                     /mol_type="genomic DNA"
                     /strain="PHL 2737"
                     /isolation_source="Abdominal ascites fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:2162637"
                     /plasmid="unnamed2"
                     /geo_loc_name="Canada: Ontario"
                     /lat_lon="43.6532 N 79.3832 W"
                     /collection_date="2018-01-01"
                     /collected_by="Public Health Ontario laboratory"
     gene            113..361
                     /locus_tag="DBK98_RS22980"
                     /old_locus_tag="DBK98_024305"
     CDS             113..361
                     /locus_tag="DBK98_RS22980"
                     /old_locus_tag="DBK98_024305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="WP_005812783.1"
                     /translation="MVTNIIQIGNSKGIILPSEVLKQLRLSLKSAVSISLDGNNIVIKA
                     QPRQGWAEAAKRAHENGDDELLIPDVFEDEKFEDWTW"
                     /name="AbrB/MazE/SpoVT family DNA-binding domain-containing
                     protein"
     gene            355..675
                     /locus_tag="DBK98_RS22985"
                     /old_locus_tag="DBK98_024310"
     CDS             355..675
                     /locus_tag="DBK98_RS22985"
                     /old_locus_tag="DBK98_024310"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799262.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003723 - RNA binding [Evidence IEA]; GO:0004521 - RNA
                     endonuclease activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="WP_005799262.1"
                     /translation="MVEQYEVYWVELDPTRGGEMAKTRPCVVVTPSDLNMYLTTVVIVP
                     ITSTIRNYPYRVLCSVAGREGEIATDQIRTVDKSRLKRKIGDLNFSEIKRLREVFQQMF
                     CE"
                     /name="type II toxin-antitoxin system PemK/MazF family
                     toxin"
     gene            712..1110
                     /locus_tag="DBK98_RS22990"
                     /old_locus_tag="DBK98_024315"
     CDS             712..1110
                     /locus_tag="DBK98_RS22990"
                     /old_locus_tag="DBK98_024315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-poly-alpha-D-galacturonosidase"
                     /protein_id="WP_128900058.1"
                     /translation="MKPTDYIEWDNLKDIPFFLCQVVEDREKQDLDIYYLGKRVLHDYY
                     HVGHYLRTAVILFRRVKSRTADWVNLRNLWTLRNCVRENYNHGIGMNDLIFGENFDGDN
                     LDTLTPLTKKRFDFLCKRIKELDPYATI"
                     /name="exo-poly-alpha-D-galacturonosidase"
     gene            1753..2655
                     /locus_tag="DBK98_RS22995"
                     /old_locus_tag="DBK98_024325"
     CDS             1753..2655
                     /locus_tag="DBK98_RS22995"
                     /old_locus_tag="DBK98_024325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_225538865.1"
                     /translation="MKAYMDAHVCSNVYTVQGGKAHSHYCHRAFCPICQRIQTAQNVKK
                     FLPVMKYLASEGREFYFVTLTLQNCVTDDARVLRDFMRRCNRMWADGIRTKHKFRSLGI
                     SGVIKKECTYHVVKKDLFSFHYHFHIIVDSLEAAKYVVAQWKKLHGSTVADARFQKYKK
                     IEDFENAAIEVFKYASKASVSKSKGRDGKKKIQINYKALDMIYTAMHGMQRMSSFGQFR
                     TMIGDEALNDFRDEDLVLNIEGLNVEDGCYTWYMSVKDKVADWYNMETGEALCCYKVTR
                     KDELLQDIYLDEDIFPNSG"
                     /name="protein rep"
     gene            complement(2659..3120)
                     /locus_tag="DBK98_RS23000"
                     /old_locus_tag="DBK98_024330"
     CDS             complement(2659..3120)
                     /locus_tag="DBK98_RS23000"
                     /old_locus_tag="DBK98_024330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005812785.1"
                     /translation="MNRIIHISLLISTVSLFLISNTGCVEKQGYYNHGEESIISLICDI
                     TWAGKKTTDENGSVWQGTYKFNKNGTYTRTNIEIDKQGNKKEANIYGQWSFGDPSFSTI
                     YFGGEHYWDIDELTKNKFSFYDRSGKFGDPFMNREYIELTPYQENNTTN"
                     /name="hypothetical protein"
     gene            3212..3982
                     /locus_tag="DBK98_RS23005"
     CDS             3212..3982
                     /locus_tag="DBK98_RS23005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016274503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005799241.1"
                     /translation="MKKVMFMFISLLTVLGVCSCNDNEVIEPVVSDSVSDMEVLSRFVD
                     VNEITNEYYFNENKKTRALSYVTGSDWQDLEKVSPLSIEKYKNNLQVLNAQVASAISNP
                     NTAYVVFSVNGKTLVKKVKEDANFDFSVFRDVVTETRAVLPSLSINGGSQSTTGVFYDS
                     SRTLKMQVDLNASIQNNYYFFEVLNPNAKPSPDDNITTPESVAFSGTGPLWSNTFTWTS
                     YWDANVPGQGFKWEFKGKGTTPSFGFIANCTFSR"
                     /name="hypothetical protein"
     gene            complement(4052..5302)
                     /locus_tag="DBK98_RS23010"
                     /old_locus_tag="DBK98_024340"
     CDS             complement(4052..5302)
                     /locus_tag="DBK98_RS23010"
                     /old_locus_tag="DBK98_024340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBU/pMV158-type mobilization protein"
                     /protein_id="WP_032535836.1"
                     /translation="MAKTCIRVEACNIGSSERHNLRSKELDYIRPELTHRNEQWVECSI
                     AEVHRDITEKYKEATGQGLQKKATPIREGVIVISEETTIQQLQDLAEKLEERFGVHAFQ
                     IYTHKDEGANVWDGKEEAWKPNYHAHMIFDWTDGHTGKTVKLNRHDMAEMQTITAECLN
                     MERGVSSDKKHLSAMQYKNKMETEKAEQLQKDIEQLNRAYTAGTEKITTVQKKLSTAQK
                     ELNSMKTDIHINEAKEAAAKAGKTVFNAVTSVFNVGEVKRQAKEIETLKKENYNLSFKN
                     QNLEGQLRTNNIEMRRVQETTDRQIKAEASKLKPITNLFPEMENAQENIEELQTMGIQN
                     KDIRQLLIGKEIYYTGNLYDKDKRKSYQVKNVKIDISKSQNGITTIWLNNIHFKNFLKE
                     LWNTLQKTLDNGNWLHR"
                     /name="NBU/pMV158-type mobilization protein"
     Typing          1607..2655
                     /name="rep_cluster_870"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.714"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4056..5302)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.76"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agccattgac agggttctcg gtgaaaatgg aaaaaagttg tttttccttt tgttattgtt
       61 ataactttac atatatttgt atcgtgataa tcaattataa agttataaca ttatggtaac
      121 gaatattatt cagattggaa acagcaaggg gataatactt ccttccgaag tattgaaaca
      181 gttgcgtttg tccttgaaat cggctgtgag catttctttg gacgggaaca atattgtgat
      241 aaaggcacaa ccacggcagg gatgggcgga agctgccaaa cgtgcccatg agaatgggga
      301 tgatgagttg cttatccctg atgtgtttga ggatgaaaag tttgaggatt ggacatggta
      361 gaacagtatg aagtgtattg ggtagagtta gaccctaccc gtgggggtga aatggcaaag
      421 acacggccat gtgtagttgt cactccttca gatttgaata tgtaccttac aacagtagtc
      481 attgttccga taacctcaac gataagaaat tatccttacc gggttctgtg ttctgtagcc
      541 gggcgtgaag gagagatagc cactgatcaa attcgcacgg tggataaaag ccggctgaaa
      601 agaaagattg gagatttgaa tttcagtgag ataaaaaggt tacgtgaagt gtttcagcaa
      661 atgttctgtg aataatggaa agtacggaaa gaaaaatgca aataagacat tatgaaacca
      721 actgactaca tcgaatggga taacctgaag gatattccat tcttcctctg tcaagtagtc
      781 gaggacagag aaaaacaaga tttggatatt tactatttgg gcaaacgtgt cttgcacgat
      841 tactaccatg tggggcacta tttgcggact gccgttatct tgttccgcag ggtaaagagc
      901 cgcacagcag actgggtaaa cctgcgtaac ctttggacgc tgcgcaactg tgtgcgtgag
      961 aactacaatc acggcatagg aatgaacgac cttatttttg gggagaattt cgatggagat
     1021 aatctcgata cgcttacacc acttacaaag aaacgctttg attttttgtg taagcggatt
     1081 aaggaacttg acccatatgc aactatttaa cactccattg agtgaaggca gggcttgtct
     1141 ttgtgtgctc cccctttggg ggacgggagg gggataagaa aaaaaatacc accggagatg
     1201 tcggatatcc gtcaattcac tccggtgttt tttttgcaac tctcccgtat cgctcggcaa
     1261 gggttgtctt tgtgtacggc aaagatagtt tttttttccg gaagtatctt tgtctgttgt
     1321 ggatttagta gtgtatttgt gtcagaattg cgcagccggg ggaatggggg cgagccccca
     1381 tactagataa actagaaagt ttcgtggtaa atgctttttg acttgttata tgtaggtttt
     1441 ttgaatgatg tttggcggtt tggaatgaat tacctacttt tgggctgaga aaaaacgaaa
     1501 gcccgaaggc agtaaccaac gggctttgta ttttaagaat tagcaatcgt ttgtgttcgt
     1561 tcttggcggg gcggatacca aacgaaagcc acatcaaaat ttcatatgtg caaagataga
     1621 acaaatataa ccaactgcaa agaaagtccg ttaaaaaacg gttctgatag tcttaaaaaa
     1681 aaggctaaag taaaaatcat atcccataat tttactcgaa aactttcgga aaagaatccg
     1741 gatagtaagc tgatgaaagc atatatggat gcccatgtgt gttcaaatgt ttatactgtg
     1801 cagggaggta aggcgcattc ccattattgt catcgggctt tttgtcctat ctgtcagcgt
     1861 attcaaacgg cacaaaatgt aaagaagttc cttccggtta tgaagtattt ggcttcggaa
     1921 ggcagagagt tttattttgt gacgcttacc ttgcaaaatt gtgtaaccga tgatgcaagg
     1981 gtattgcgag atttcatgcg tagatgtaac cggatgtggg ctgatggtat aaggacgaaa
     2041 cacaagtttc gttcacttgg tatttcgggg gtgattaaga aggagtgtac ctatcatgta
     2101 gtaaaaaagg atttgttttc gttccattat cattttcata ttattgttga ttcattggaa
     2161 gctgcaaaat atgtagtagc ccaatggaag aaattgcatg gcagtacggt tgctgatgcc
     2221 cgttttcaga aatataagaa gatagaggat tttgaaaatg cagcgataga ggtttttaag
     2281 tatgcttcaa aggcttctgt ctcaaaatcg aaaggtcgtg acggtaaaaa aaagattcag
     2341 ataaattata aggctttgga tatgatttat actgccatgc atgggatgca gcgcatgagt
     2401 agtttcggac agttccgcac catgatcgga gatgaagcgt tgaatgattt tagagatgag
     2461 gatttggttc ttaatattga gggattgaat gttgaagatg gttgttatac ttggtatatg
     2521 tctgtgaaag ataaggtggc tgattggtat aatatggaaa ctggggaggc attgtgttgt
     2581 tataaggtca cccggaagga tgaacttttg caggatattt atttagatga ggatattttt
     2641 cctaatagtg ggtagttgtt agtttgttgt attgttttct tggtatggtg tgagttctat
     2701 gtattctcta ttcatgaaag gatcgccaaa ttttccactt ctgtcataga aggaaaattt
     2761 gtttttagta agctcgtcta tgtcccagta atgttctcca ccaaaataaa tggtactgaa
     2821 acttggatca ccgaatgacc attgtccgta gatattggct tcttttttat ttccttgttt
     2881 gtcgatttct atatttgtac gggtatatgt tccgttttta ttgaatttgt atgtaccttg
     2941 ccagacactg ccgttttcgt ctgttgtttt tttaccagcc catgtaatat cacaaattaa
     3001 tgagattatg ctttcttctc catggttgta gtatccttgt ttttcaacac atccggtgtt
     3061 tgaaataaga aaaagtgata ctgttgaaat aagtaaagag atatgtatta ttctattcat
     3121 agttaaatat ttaatattca atgcaaaaat aggagaaatt ttatattttt gggcgaaacc
     3181 tttatttatt aactttaaaa aaggaggatt tatgaaaaaa gtaatgttta tgttcatcag
     3241 tctactgact gttttgggtg tttgttcgtg taatgataat gaagtaatag aacctgtggt
     3301 tagtgattct gtatctgata tggaggtctt atctcgtttt gttgatgtaa atgagattac
     3361 aaatgaatac tatttcaatg agaataagaa aacgagggcg ttgtcttatg taacaggttc
     3421 ggattggcag gatttagaaa aagttagtcc tttgagtatt gagaaatata agaataacct
     3481 gcaggtttta aatgctcaag tggcaagtgc tatttcaaat cctaataccg cttatgttgt
     3541 tttttctgta aatggaaaga ctttggttaa aaaggtgaag gaggatgcta attttgattt
     3601 ttcagtattt agggatgttg taacggaaac gagggctgtt ctcccttctt tgagtattaa
     3661 tggaggttct caaagcacta ccggagtttt ttacgattcc agtcgtactt taaagatgca
     3721 agttgatttg aatgcttcaa tacagaataa ttattatttc tttgaagtat taaatcctaa
     3781 tgctaaacca agtccggatg ataatataac gacacctgaa agtgttgctt ttagtggaac
     3841 tggaccgtta tggagtaaca ctttcacttg gacttcgtat tgggatgcaa atgtacctgg
     3901 tcaaggtttt aaatgggagt ttaaaggaaa aggtactact ccttcttttg gttttatagc
     3961 taattgcacc tttagtcgtt aaaaaatgaa cgggatttaa tttgaattgc acttctcaaa
     4021 aaagagaagt gcaattcttt ttagtgtctt tctatcggtg tagccagtta ccgttatcta
     4081 aagtcttttg tagtgtattc caaagttctt taagaaagtt tttaaagtgg atgttattta
     4141 gccaaattgt agtaatacca ttctgacttt tggagatatc tattttcacg tttttaactt
     4201 ggtatgattt tctcttgtct ttatcgtaaa ggtttccagt gtagtatatt tcctttccaa
     4261 taagaagttg tcggatatct ttattttgta ttcccatagt ttggagttcc tctatatttt
     4321 cctgtgcatt ttccatttcc ggaaaaaggt tagttatggg ttttagtttg gatgcttctg
     4381 cctttatctg tctgtcggtt gtttcctgta cccttcgcat ctcgatgttg ttggttctta
     4441 attgtccttc taaattttga ttcttgaatg agaggttata attttctttt ttgagggttt
     4501 caatttcttt ggcttgtcgt tttacttccc ctacattaaa gacgcttgta acggcattaa
     4561 atacggtttt tccggctttt gcggcagctt ctttggcttc gttgatgtgt atgtcggttt
     4621 tcatgctgtt tagctctttt tgtgccgtac taagtttctt ttggactgtg gtgattttct
     4681 cggttccggc agtgtatgcc ctgtttagtt gctcgatgtc tttttgaagt tgttcagctt
     4741 tctctgtttc cattttgttt ttgtattgca tggccgacaa gtgtttcttg tctgaagaaa
     4801 ctccacgttc catgttgaga cactctgctg ttatagtctg catctccgcc atgtcgtgtc
     4861 tgttcagctt tacagttttt cctgtatggc cgtccgtcca gtcaaagatc atgtgcgcat
     4921 gatagttcgg tttccatgct tcttcctttc cgtcccatac gtttgcgcct tcatccttat
     4981 gggtgtagat ttggaaggcg tgcactccaa agcgttcttc cagtttttcg gccaagtctt
     5041 gaagctgctg gattgtggtt tcctcgctga ttactattac cccttcccgg attggggtag
     5101 ctttcttctg taatccttgt ccggtggctt ccttgtattt ttccgttatg tcccggtgta
     5161 cttcggcaat ggaacattct acccactgtt cgttgcggtg tgtgagttcc ggacggatgt
     5221 agtcgagctc cttgcttctt aagttgtgac gttccgagct gcctatattg caggcttcca
     5281 ctctgataca tgtctttgcc atatctttag gttttaggtg tgtatgtatg tttagaagat
     5341 gtggcgtgaa acgtcacatc gcacggggtc gaggggacac ccctcgcaca caaccccttt
     5401 aggggttata gtgtgctata accttttgca aaggtaagcc ttccggacgg gatttgcaag
     5461 cgctctgcca tgtgttccgg atgtgcttgg gaacagaagc cggacaaaag ggaaaaggac
     5521 acaaggcaca ataaaaaatc atcggtcggg gagcgctgca cggcaagggg gtgcaccaac
     5581 actcccgacg agtgc
//
