LOCUS       NZ_CP040599            11025 bp    DNA     circular CON 08-JAN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain KpvST15_NDM plasmid
            unnamed4, complete sequence.
ACCESSION   NZ_CP040599
VERSION     NZ_CP040599.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN11793565
            Assembly: GCF_005885775.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11025)
  AUTHORS   Turton,J.F., Davies,F., Turton,J.A., Perry,C.L., Payne,Z. and
            Pike,R.
  TITLE     Hybrid resistance and virulence plasmids in high-risk clones of
            Klebsiella pneumoniae, including those carrying blaNDM-5
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11025)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2019) Healthcare Associated Infection &
            Antimicrobial Resistance Division, Public Health England, 61
            Colindale Avenue, London, London NW9 5EQ, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 10-MAY-2019
            Assembly Method :: miniasm v. 0.2-r168-dirty; racon correction (3
            iterations) v. 1.3.1; racon correction with Illumina reads v. 1.3.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/08/2024 05:09:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,771
            CDSs (total)                      :: 5,646
            Genes (coding)                    :: 5,328
            CDSs (with protein)               :: 5,328
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 318
            CDSs (without protein)            :: 318
            Pseudo Genes (ambiguous residues) :: 0 of 318
            Pseudo Genes (frameshifted)       :: 200 of 318
            Pseudo Genes (incomplete)         :: 128 of 318
            Pseudo Genes (internal stop)      :: 35 of 318
            Pseudo Genes (multiple problems)  :: 42 of 318
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP040599.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11025
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KpvST15_NDM"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="unnamed4"
                     /geo_loc_name="United Kingdom"
                     /lat_lon="51.55 N 0.04 W"
                     /collection_date="2016-12-19"
                     /collected_by="Hospital in London"
                     /genotype="ST15"
     gene            complement(join(10204..11025,1..27))
                     /locus_tag="FFY44_RS29140"
                     /old_locus_tag="FFY44_29140"
     CDS             complement(join(10204..11025,1..27))
                     /locus_tag="FFY44_RS29140"
                     /old_locus_tag="FFY44_29140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005325933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="WP_138663974.1"
                     /translation="MQRSCNAGSLRSVGTVRNYEQGLTRVAEWMKDNRINHGDLRTLTP
                     AQAITYLEQRGEQVGQKTLDMERQAIQAMMIHVTQTLPAGEHLPVVKSEQAQILSSRAY
                     SPDQVALIAAAQQPANALATAIALAAGLRAHELLTLAPRQERAPDTRPAADSKFAGREG
                     VIYTVQGKGGLVREVLIPSPLAERLEHVRLASPVRVTDRGVFYQSQYAIGGGQRWSNAF
                     SAASMRTLGWSRGAHGVRHSYAQQRMQELQKLGLVRDMALRTVSQEMGHFRPEITETYL
                     R"
                     /name="site-specific integrase"
     gene            complement(join(10946..11025,1..160))
                     /locus_tag="FFY44_RS29145"
                     /old_locus_tag="FFY44_29145"
     CDS             complement(join(10946..11025,1..160))
                     /locus_tag="FFY44_RS29145"
                     /old_locus_tag="FFY44_29145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010848996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_138663975.1"
                     /translation="MQFDTAAELAALQAQTRRIRQVRYRPSRLDRYTGELLSLYQAGAS
                     AAELQRWLPALCRHSPELRAGPDPRRRMDERQPD"
                     /name="hypothetical protein"
     gene            740..946
                     /locus_tag="FFY44_RS29150"
                     /old_locus_tag="FFY44_29150"
     CDS             740..946
                     /locus_tag="FFY44_RS29150"
                     /old_locus_tag="FFY44_29150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016162092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_047359279.1"
                     /translation="MKLITVDESVVNAIVEYYAQGSEEAHAEGNRMFLRYLHELTGTQR
                     VAVEAARKQSLAAINKGGALPCD"
                     /name="hypothetical protein"
     gene            complement(1893..2577)
                     /locus_tag="FFY44_RS29160"
                     /old_locus_tag="FFY44_29160"
                     /pseudo=""
     CDS             complement(1893..2577)
                     /locus_tag="FFY44_RS29160"
                     /old_locus_tag="FFY44_29160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /name="IS1 family transposase"
     gene            3385..4608
                     /locus_tag="FFY44_RS29165"
                     /old_locus_tag="FFY44_29165"
     CDS             3385..4608
                     /locus_tag="FFY44_RS29165"
                     /old_locus_tag="FFY44_29165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013512451.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="WP_227513120.1"
                     /translation="MMAEKLVSIIGLVFINSYMAKYIGPTDFGKITFATTIFTFVQTMA
                     WFGGQNILFKRMSEKRKSGIALALHTQKQRRVIFALSSLIALFYLFFFTDTLTFVFGVA
                     NCIATYYIVMDVFAVYNNTQLISRINALTNGAGLCIAFIVRFLISYLELPVYTFSIPII
                     LIPAIPYVIRYIYFHNTIEDTAVLKSKTKKYNKYLFSAGGSLLLSTLSIVIYTQVSNIF
                     LAKITSYSDLGIYNVALTLGSAYSFIPVALITSFFSKIYQEKDFEKMSRLLTLINRIVI
                     LITIFILFGFYFLGPYIISMLYGSAYKLAVELVPIIILATMFSSLGTICYRFIIKYSGY
                     KYLSYKMTLTSLLSIPFSYVMINRYDVFGAAYCFLVVEVLSCTLLNYFFRNGMILRIHL
                     NVIGLMRR"
                     /name="oligosaccharide flippase family protein"
     gene            4889..5767
                     /locus_tag="FFY44_RS29170"
                     /old_locus_tag="FFY44_29170"
     CDS             4889..5767
                     /locus_tag="FFY44_RS29170"
                     /old_locus_tag="FFY44_29170"
                     /inference="COORDINATES: protein motif:HMM:NF025663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp fold amidoligase family protein"
                     /protein_id="WP_158236426.1"
                     /translation="MIKFFFGKAYRLLLENLSTTNRVKLRYFRVYKKLPNIKKPKTFNE
                     KVISRILGEQKDIYSTLADKYLVRRFVSERVGEEYLIPLHLATSNPSDLLKIDDWSGLV
                     IKPNHAAGMIYISDDNPSKEEKDNVVTTAKTWLKTDFSSFCDEMHYSKIKPMLLVERKI
                     TKRGEIPRDYKFHCFKQKNGDINFVLQLVDGRFGQESRSYFLNSFENMVWSHGAGNHLL
                     SNNEVAVLNEVILLNKRLILNDFNYVRIDWYILGDRIYFGEITFTPGAGIGNEFGPALE
                     KQMAEWWEDEY"
                     /name="ATP-grasp fold amidoligase family protein"
     gene            complement(6176..>7143)
                     /locus_tag="FFY44_RS29175"
                     /old_locus_tag="FFY44_29175"
                     /pseudo=""
     CDS             complement(6176..>7143)
                     /locus_tag="FFY44_RS29175"
                     /old_locus_tag="FFY44_29175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341074.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /name="IS3 family transposase"
     gene            complement(<7309..7931)
                     /locus_tag="FFY44_RS29180"
                     /old_locus_tag="FFY44_29180"
                     /pseudo=""
     CDS             complement(<7309..7931)
                     /locus_tag="FFY44_RS29180"
                     /old_locus_tag="FFY44_29180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011073174.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /name="IS5 family transposase"
     gene            complement(8383..8496)
                     /locus_tag="FFY44_RS30210"
                     /old_locus_tag="FFY44_29190"
     CDS             complement(8383..8496)
                     /locus_tag="FFY44_RS30210"
                     /old_locus_tag="FFY44_29190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032634168.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAATGN"
                     /name="hypothetical protein"
     gene            8907..9098
                     /locus_tag="FFY44_RS29200"
                     /old_locus_tag="FFY44_29200"
     CDS             8907..9098
                     /locus_tag="FFY44_RS29200"
                     /old_locus_tag="FFY44_29200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_016947206.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSLRLDALQEGD"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            9098..9502
                     /locus_tag="FFY44_RS29205"
                     /old_locus_tag="FFY44_29205"
     CDS             9098..9502
                     /locus_tag="FFY44_RS29205"
                     /old_locus_tag="FFY44_29205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016162088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_064179686.1"
                     /translation="MAKVSISEAARLTGKSRTTLHRLIKAGELSTCRGERNAKMLDTSE
                     LLRVFGAFVPVSVEQEGEQVSGQRDTVTSDRNEQVIYILKQEIEHLNTLLASRDSHIDS
                     LKQAMLLLENREEKRQNTTRPRAWWRFWQS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(9509..10207)
                     /locus_tag="FFY44_RS29210"
                     /old_locus_tag="FFY44_29210"
     CDS             complement(9509..10207)
                     /locus_tag="FFY44_RS29210"
                     /old_locus_tag="FFY44_29210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016162089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_064179687.1"
                     /translation="MTRTFILLCTGVFAIAYALYELKHMGVYYPDASNPQLFTGWKAVT
                     ELGPAILATGIAATAFSLWLFILFFRGSESRRIQREISGYQQALREAEEAHRARQNTLI
                     QNHNLETAALRSHYSGAEDVLREAFAKKENRWRQALKNAREEARQELEEQTEALNQRAS
                     GLLVQEQTLKARMEEVRKYVAGERHNAMRARLEAEEAEKRRQNASATAERRRRKLEKLQ
                     ATDGTAGQKH"
                     /name="hypothetical protein"
     Typing          complement(8384..8859)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.286"
                     /coverage="92.0"
                     /evalue="0.0"
ORIGIN
        1 ggagccagcg ttgcagctcc gctgcactgg ccccggcctg gtacaggctc agcagttcgc
       61 cggtatagcg gtcaaggcgg gagggacggt agcggacctg gcggatacgg cgggtctgcg
      121 cctgcagggc ggccagttcg gcagcggtat caaattgcat ctgtgctccg gattgggtgt
      181 aagaacatca gtggccgtta cgttaacggt cactgcggca tgcgccgatc gccctcgttc
      241 gaacctctcg tggttcagcc cggactgctc gcggctgata acgtccctta agccagggaa
      301 tggcctgacc tgattgccgg tctcgcagat tttagctgtt ttctcaggat tctcggattg
      361 ggctgcccgg tacagtatcc ccggagggac ccggctgttt tacctgctgc cggtaggcag
      421 gtgcgcgtca gagtgcgcct cctcatggtt tgactaaata ctagccaggt ttttcgtgcg
      481 tgtgaatgtt gttttttaaa tgttgcgcct gactccgctc aatagcgaca cccgctatgt
      541 acaggcgcat tttatgcgtc gtacatagcg ggtgtcgcag gtgctgattt ggcggaataa
      601 aaaccgccgg gactgaccgc cccggacccc gccaacggtg accggacaga caaaaaattt
      661 gcctgcccgg tcaccgtttt cacgccagga tgaccgtcgt gatgccagga tggtgtcaca
      721 gagaaagcgg agagacagaa tgaagctgat tacggtggat gaatccgtgg tgaacgcgat
      781 agtggaatac tacgcacagg gcagtgagga ggcgcatgca gagggaaaca ggatgttcct
      841 gcgttatctg cacgagttaa cgggaacaca gcgtgtggcg gttgaggcgg ccagaaagca
      901 gtctctggcc gcgataaata aggggggggc gcttccctgt gattaaaatc gcatttatgg
      961 acggctgatg tctgtgcact ggcccggaaa tatttttctc cggtgagggg ctgatatgag
     1021 ctaccttttt cttgtcgccg gcaccgcacc aggtgcgacg gtccttttac agagttgtcc
     1081 tcatttgacg ggcctggatg gcccgttctt ttatgctttt aacgtccggg gcccttttct
     1141 cggccgaaat caccggctat gcggtggtgt gcatagccat gcatatgcgt aaaataaatc
     1201 aggcgcgttt ctggcgggtt ttcgggtggt ttgttgcctg ttttaccggt ttcctgccag
     1261 aaacgccctg aggccgtttt cgcggtgcgc gtaagaaggg attatggtaa atagggcgct
     1321 atcgcctaaa tacacggctg attgattttt tttctctgaa gtatgagatt cctgagtaga
     1381 cagctacaac aagtagagca atggcgatag caagtaaaat ccagtccata aattactgat
     1441 taatttaaca aaaacagcct gttatgattt cataaagata atatcatctt ttttttagca
     1501 tgatactgtc gttacttatt gtaatgagat gaagcataac cgctgtttgt gtgtgtgttt
     1561 gttaaggctg ctaagattat tcctagttat gttttttgta catacaggaa aaacaaaaaa
     1621 accgtgcatg agttatgggc atgttccccc ccctgcttgt ctgcaaccac tgccgtaaaa
     1681 cgcggcagcg gcagttaact tacactgcct attggggcgt tacccaacgc tcatccggag
     1741 catccggatt gaactgtcgc tggagcctgt tgggcgacac gatactggcc tcgcctttac
     1801 gtgcccgcgg gatccggtat ccggaccgta cttctttatc caggcgtaaa ggctgtgggt
     1861 ggtgatatcg agacgtgggt aatgactcca acttactgat agtgttttat gttcagataa
     1921 tgcccgatga ccttgtcatg ctgctccacc gattttgaga acgacagtga cttccgtccc
     1981 agccttgcca gatgttgtct cagattcagg ttatgtcgct caatgcgctg agtgtaacgc
     2041 ttgctgttaa cgtgcagctt tcccttcagg cgtgattcat acagcggcca gccatccgtc
     2101 atccatacca cgacctcaaa ggccgacagc actccagtgt ggccagagtg cgttcaccga
     2161 agacgtgcgc cacaaccgtc ctccgtatcc tgtcatacgc gtaaaacagc cagcgctgac
     2221 gtgatttagc accgacgtag ccccactgtt cgtccatttc agcgcagaca atcacatcac
     2281 tgcccggttg tatgcgcgag gttaccgact gcggcctgag ttttttaagt gtcttaaaat
     2341 cgtgttgagg ccaacgccca taatgcgtgc actggcgcga catccgacgc cattcatggc
     2401 catatcaatg attttctggt gcgtaccggg ctgagaggcg gtgtaagtga actgtagttg
     2461 ccatgtttta cggcaatgag agcagagata gcgctgatgt ccggcagtgc ttttgccgtt
     2521 acgcaccacg ccttcagtag cggagcagga aggacatctg atggaaatgg aagccacgca
     2581 agcaccttaa aatcaccatc atacactaaa ttagtaagtt ggcagcatta ccttaaatta
     2641 tgtgttccat aggctctgtg atcaggtttt caacctgatt caggtcatgt acattcgcag
     2701 cggtggccac cacttttttg ttaattctgt aattgcatca acggcgatgt tagtccactg
     2761 agtcagtata ctgataagtg taaaggaatc ggaatttgca gaaaatggca agtctcgtga
     2821 aagttccatg ataagaaaac gttacgccat cataagcaag ggtgaaatcg gtccttcctg
     2881 aagttatcgt gtttattgtt aagttactta atgatttgac ctcaacccaa atgttaaaga
     2941 tagtcgttag cgttagtgca gtggataggt agctgaagag cctagggcgg tagcgtgcct
     3001 aaattgttgt tttttatagg ttttattcaa tttataagat gtagaagata tgtcaaaaga
     3061 gagcttttag gtggattctt tgcagtagtt agaccacgac aaaaatcagc ttttgaatcg
     3121 ccacggataa tctagacact tccgagccgt tgataatact ggttttcata ttctgtcggt
     3181 gacatctgat cgctggaacc atgccgacgc tggtaatgat accaacttgt tgatttcgta
     3241 ttttcactga cactaggatt atcctgatgt tacaaggatt gaatgactac agattaaaat
     3301 agttatcaac aagttgacag cattaccaaa atttctatgg gattaatttt ttgcgagtga
     3361 gtaatgtatt ctaatgcctt ttggatgatg gctgaaaaat tagtcagcat catcggtctt
     3421 gtgtttataa actcctatat ggcaaaatat ataggaccta ctgattttgg taaaattact
     3481 tttgctacga caatatttac gtttgtacag accatggctt ggtttggtgg gcaaaacata
     3541 ctttttaaaa ggatgagtga gaaaagaaaa tcaggtattg ctcttgcatt acatactcaa
     3601 aagcagcgca gggttatttt tgcactctct tcattgattg ccttgttcta tttgtttttt
     3661 tttacagata ccctgacatt tgtttttggg gtggctaact gcattgccac ttattatata
     3721 gtgatggatg ttttcgctgt atataataat acgcagttaa tatctagaat taatgcatta
     3781 acaaatggtg cagggttgtg tattgctttt atagtccgct ttttaatttc atacttggag
     3841 ttgccagtat atacatttag cattcctatt atattaatcc ctgcaatacc ttatgttatt
     3901 agatatatat actttcacaa taccattgaa gacactgcgg tattaaaatc gaaaacgaag
     3961 aaatataata agtatttgtt ttcagcggga gggtcgttgt tactatcaac cctgtccatt
     4021 gtgatttaca cacaggtatc aaatattttt ttggctaaaa taacctcata ttctgatttg
     4081 ggtatttata atgtggcgct gacgcttggc agtgcgtatt catttattcc agttgcgtta
     4141 atcactagtt ttttttctaa aatctatcaa gagaaagatt ttgaaaaaat gtctaggctt
     4201 ctaactttaa taaatagaat tgtgatcttg ataacaattt ttatattatt tggtttttat
     4261 tttcttgggc cgtacattat cagcatgctc tatggttcgg cctataaact ggctgttgag
     4321 ttggtgccaa tcattattct agcgacaatg ttttcatcct tgggaactat ttgttataga
     4381 ttcataataa aatactctgg ctacaaatac ctttcatata aaatgacgtt aactagcttg
     4441 ctcagcatcc cattctcata tgttatgatt aaccgttatg atgttttcgg tgctgcgtat
     4501 tgttttcttg tggtcgaagt tctgtcatgc acattattaa attatttctt taggaatgga
     4561 atgattctaa ggattcattt aaatgttatt gggttgatga ggcgttgatg gtgggggggt
     4621 agatttattt atggattgtg ttttattgat tttcttatga aatttaaaat atgatgatta
     4681 gttctggtgt gttgggggtg agtttttgaa ttttattgtt acttatgatt ttttattatt
     4741 tatctgtatg tttaataaga ttgagtgtgt agactgagtt ccgtggaacc cttatctgca
     4801 gtgcatcaag aagccaaggc gcatgaaact tggagtgtct cgagtataca tgctttctga
     4861 tttaacagat taaaaagaga gaattgaagt gattaagttt ttttttggta aggcttacag
     4921 gttgcttttg gagaatttgt ctacaaccaa tagagttaaa ctaagatatt ttagagtgta
     4981 taaaaagctt ccaaatataa aaaaacctaa aacctttaat gaaaaggtta ttagtagaat
     5041 actaggagaa caaaaggata tatactccac attggctgat aaatatcttg tgcggcgttt
     5101 cgtatccgag cgggttggag aggagtatct tattcctctc catttggcta ctagcaaccc
     5161 tagtgactta ttaaaaatag atgattggtc tggattggtt atcaaaccaa atcatgcggc
     5221 aggcatgatt tatatatccg atgacaatcc ctctaaagag gagaaagaca atgttgttac
     5281 cactgcgaaa acctggttga aaactgactt ttcctctttt tgcgatgaaa tgcattactc
     5341 caagataaag ccaatgcttt tagtggagag gaaaataaca aaacgtggtg aaatccctcg
     5401 tgattacaag tttcattgtt tcaaacaaaa aaatggtgat attaattttg tgttgcagtt
     5461 agtggacggt cgctttgggc aggagtcacg aagctatttt ttaaattctt ttgaaaatat
     5521 ggtttggagt catggcgctg gcaatcattt attaagtaat aatgaggttg ctgttttaaa
     5581 tgaagtgatt ttattaaata aaagacttat ccttaatgat tttaattacg tacgtattga
     5641 ttggtatatt ttaggtgacc gaatatattt tggtgagatt acatttactc caggggctgg
     5701 aattggaaat gaatttggac ctgccttgga aaaacaaatg gctgagtggt gggaggatga
     5761 atattaattc atgcactacg ctcgtggtat ttgcaagttg catgcgctat ttcttcttga
     5821 ttatcgcctt tggctgaagg tactttttac aagcaaggtg atcttttgat gatgttctta
     5881 cctactttcg aaacctgatc gtttttcctt gagattggag tcactgcatc gtaatctaag
     5941 tagcagaaaa gaaaaaacca cctggggtgg tggttttttc gatgggtcag taagttgggg
     6001 aacttctgaa ccgtggtaac agggtgtaca agaccgctgc aacctaattt gtccttacag
     6061 tttagccgca gtaaggcttt aacttcaaga actctgagtc agtaaatctt gtacacccgt
     6121 taccagtggc gatgtatcgt gtccgtaagg gttggactga atcgccacag ataatctaga
     6181 cacttccgag ccgttgataa tactgttttt catattctgt cggtgacatc tgttcgctag
     6241 aaccatgccg acgcttactg ttataaaaca tttcgatgta atcaaaaata tcactgcggg
     6301 cttcttctcg cgttccgtag atctttttct ttatccgttc acgtttcaac aactggaaaa
     6361 aactttctgc aaccgcatta tcatggcagt taccgcgacg gctcatgcta ccctccaggc
     6421 cgtgtgattt caggaacgac tgccactcat ggcttgtgta ctgactgccc tgatccgaat
     6481 gaaccagcac ctgtttttcg ggattacgcc gccatacagc catcagcagt gcgttcagga
     6541 caatgtcctt tgtcatccgg gattgcatgg accagccgat aattttgcgt gagaacagat
     6601 caacaacaac ggcaagatac agccagcctt cgtgggtcct gatgtaggtt atgtccgtta
     6661 cccaacgctc atcaggagca tccggattga actgtcgctg gagcctgttg ggtgacacga
     6721 tactggcctc gcctttacgt gcccgcgggc ttcggtatcc gacctgagcc tttattccga
     6781 cacgtttcat cagtctccag actctgttta ttccgcactg ttgcccgctg tcacgcagat
     6841 ccagatggat tttgcgataa ccatagacgc atcccgattc cagccagaac tgtttaatct
     6901 gtcctgtcag tctcaggtct gcctgatggc gttgtgaatg cggctgctga agccaggcgt
     6961 aaaaaccact gggatgaaca tccagcaccc gacagagcag gcgaacaggc cagcaacagg
     7021 agttgtcacg gataaaggcg taccttagtc ggacagtttt gcgaagtacg ccgcggcttt
     7081 ttttaatatg tcccgttcat cggtaacccg tttcagctct ttctggagac ggcggatctc
     7141 ggcctgagtg atcttgaccc gccatctccg gacagctttt gtatcttaag ttaacgatgc
     7201 ccgcagttgc cggtattctc tcggagagtg atatcccagc gcgctgggcg ggtggttttc
     7261 attgtaatgc gtgaacgctg ctgcaaggtt tcgcagggct gttcccacac ccggccagta
     7321 gcccgctccc tttcggggag gaatgagcgc gctgattttc ttgcgacgca gttcgtcatg
     7381 acatcgctgc gtatcgtaag cgccgtccgc cgccgctgac ctgatttttc ggtgggtctg
     7441 ccggataagc cccgggaaag cttcagcgtc cgtgacgttg ttcagtgaca ggtccgagca
     7501 gatggcttca tgcctgctcg cgtcaacagc cagatgcagt tttcgccaga tacgacggcg
     7561 ctctttgccg tgctttttga ctttccactc accttcaccg aaggctttca gccctgtgga
     7621 atcaatcacc agatgcgaaa tttcaccccg ggtgggcgtt tgaaactgac gttaaccgac
     7681 tttgcccgct tgctgacact ggtgtaatcc ggacagcgca gcgggacatc catcagggca
     7741 aaaatggaat caataaagcc ctgagcggtt cgcagggtca gccggaatac acgtttaatc
     7801 accaggaagg ttgtgatggc aggggctgta tagcgctgag gttgtccccg cgatgcaggc
     7861 gttgctgact cataccaggc cagagtcgcc tcatcatcaa cccagaaagt gatggagtca
     7921 cgattaatca gggccttgtt gtagttgcgt cagttggtga ttttgaattt ctgctttgcc
     7981 acggaactgc ctgcatagtc aggataatgc gtgatctgat tcttcaggtt accaaaattt
     8041 cgatttattc aacaaagcca ctgattgtgt aaaatcttac tctatattca ttttttttaa
     8101 taagatcaaa tgcttttgtt ttctgttagt tcactggcgt cgctggtttc ccttcagggg
     8161 aaggtatatt gaacaggatc ctcttgagat cctttctttg gcatcgtaat ctcttgctct
     8221 gtaaacgaaa aaaccacccg gcaaggtggt tttttcgaag gttcagcaac cggcaagttt
     8281 ctgaaccgag gtaactggct aagatagagg agcgcagtca ccaaaactgt tcgtttagtt
     8341 tagccttaac tggcacatga cttcaagtcc atctcctcta aatcagttac cagtggctgc
     8401 tgccagtggc gcttttgcat gtctttccgg gttggactca agacgatagt taccggataa
     8461 ggcgcagcag tcggactgaa cggggggttc gtgcatacag tccagcttgg agcgaactgc
     8521 cttcccggaa ctgagtgtca ggcgtggaat gagataaacg cggccataac agcggaatga
     8581 caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccacca gggggaaacg
     8641 cctggtatct ttaagtcctg tcgggtttcg ccaccgctga tttgagcgtc cgtttctgtg
     8701 atgcttgtca ggggggcgga gcctatggaa aaacggcaac gccgtggctt tttccacagg
     8761 ctcttttctt cccggttatc gcgatgactc ttctgtgtgt cccgtccgct cgccgcagcc
     8821 gaacggcagg agcgtagcga ctgagtgagc gaggaagcgg aaaagagacc ggttttacac
     8881 tgagcactga cgccctgagg ccccttatga cgaaacaaca gaaaactgcc ctgaacatgg
     8941 ctaaattcat ccagaatcag tccctgctcc tgctggagaa gcttaacgag cttgatctcg
     9001 atgcggaggc tgacctttgt gagaaactcc acgatgatgc ggagcatctt ttccgtacac
     9061 tgtccctcag actggatgca ctacaggagg gtgactgatg gcgaaagtgt cgatcagtga
     9121 ggctgcacgc ctgacaggta agagcaggac aaccctgcac aggttaatta aagccggtga
     9181 actgtccacc tgtcggggtg aacggaatgc gaaaatgctt gatacttcag agcttttgcg
     9241 ggtgtttggt gcctttgtgc cggtatccgt tgaacaggag ggagaacagg tcagtggaca
     9301 gcgtgacaca gtcacttctg accgtaatga acaggtcatt tacattctga aacaggaaat
     9361 tgaacacctg aacaccctcc ttgcctccag agattcacat atcgacagtc tgaaacaggc
     9421 gatgttgttg ctggagaacc gggaagagaa gcggcagaac acgacccggc cccgtgcctg
     9481 gtggcgtttc tggcagtcct gaaatgcctc agtgtttttg ccctgctgtt ccgtcagtgg
     9541 cctggagttt ttccagtttc cgtctgcgcc gttccgctgt tgcactggca ttttgtcgcc
     9601 ttttctctgc ttcctctgcc tccagacggg cccgcatggc gttatgccgc tctccggcaa
     9661 catactttct gacttcctcc attctggctt tcagtgtctg ctcctgtacc agcaggccgg
     9721 acgcgcgctg attcagtgcc tccgtctgtt cttccagctc ctgcctggct tcttcccggg
     9781 catttttcaa tgcctgacgc cagcggtttt cttttttcgc aaacgcttcc cgcagtacat
     9841 cctcagcacc actgtagtgg cttcgcaggg ccgctgtctc caggttgtga ttctgaataa
     9901 gggtattttg tctggctctg tgagcctctt ctgcctccct tagtgcctgc tgatatccgc
     9961 ttatctcgcg ctgtatccga cgggactctg agcctctgaa aaacaaaatg aacagccaca
    10021 gcgagaacgc tgtggctgcg atccccgtag ccaggattgc gggaccgagt tcggtgaccg
    10081 ccttccagcc agtgaacagc tgcggattgc tggcatccgg atagtaaacg cccatatgtt
    10141 tcagctcata cagggcgtac gcgatggcga aaacaccggt acacaataaa ataaacgttc
    10201 ttgtcatcgc aggtatgtct ccgtgatttc cggacgaaaa tggcccattt cctggctcac
    10261 ggtacgcagg gccatatcac gaaccagacc gagtttttgc agctcctgca tccgttgctg
    10321 ggcgtagctg tgacgcacgc cgtgtgcccc ccgtgaccag cccagggtac gcatggaggc
    10381 ggcactgaag gcgttcgacc agcgctgtcc tccgccaatg gcgtactgac tctggtaaaa
    10441 cacgccgcga tcggtcaccc ggaccgggct cgccagcctg acatgctcga gacgttcagc
    10501 caggggggac ggtatcagga cttccctgac caggccgcct ttgccctgaa cggtgtaaat
    10561 cacaccctcc cgaccggcaa atttactgtc cgctgccgga cgggtatccg gtgcacgctc
    10621 ctggcggggc gcgagggtca gcagttcatg ggcgcgtaat ccggcggcca gagcaatcgc
    10681 ggtggccaga gcgttggcgg gctgttgtgc tgcggcaatc agggctacct ggtccgggct
    10741 gtaggcccgg ctgctcagga tctgtgcctg ctccgatttc accaccggca ggtgttcgcc
    10801 ggcagggagg gtctgcgtca cgtggatcat catggcctgg atggcctggc gttccatatc
    10861 gagcgttttc tggccgacct gctccccgcg ctgctccagg taggtgatgg cctgcgccgg
    10921 tgtcagtgtg cgtaaatcgc cgtgattaat ccggttgtct ttcatccatt ctgcgacgcg
    10981 ggtcaggccc tgctcgtaat tccggactgt gccgacagag cgcag
//
