LOCUS       NZ_CP040596             9571 bp    DNA     circular CON 08-JAN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain KpvST15_NDM plasmid
            unnamed2, complete sequence.
ACCESSION   NZ_CP040596
VERSION     NZ_CP040596.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN11793565
            Assembly: GCF_005885775.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9571)
  AUTHORS   Turton,J.F., Davies,F., Turton,J.A., Perry,C.L., Payne,Z. and
            Pike,R.
  TITLE     Hybrid resistance and virulence plasmids in high-risk clones of
            Klebsiella pneumoniae, including those carrying blaNDM-5
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9571)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2019) Healthcare Associated Infection &
            Antimicrobial Resistance Division, Public Health England, 61
            Colindale Avenue, London, London NW9 5EQ, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 10-MAY-2019
            Assembly Method :: miniasm v. 0.2-r168-dirty; racon correction (3
            iterations) v. 1.3.1; racon correction with Illumina reads v. 1.3.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/08/2024 05:09:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,771
            CDSs (total)                      :: 5,646
            Genes (coding)                    :: 5,328
            CDSs (with protein)               :: 5,328
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 318
            CDSs (without protein)            :: 318
            Pseudo Genes (ambiguous residues) :: 0 of 318
            Pseudo Genes (frameshifted)       :: 200 of 318
            Pseudo Genes (incomplete)         :: 128 of 318
            Pseudo Genes (internal stop)      :: 35 of 318
            Pseudo Genes (multiple problems)  :: 42 of 318
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP040596.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9571
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KpvST15_NDM"
                     /isolation_source="throat"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="unnamed2"
                     /geo_loc_name="United Kingdom"
                     /lat_lon="51.55 N 0.04 W"
                     /collection_date="2016-12-19"
                     /collected_by="Hospital in London"
                     /genotype="ST15"
     gene            32..211
                     /locus_tag="FFY44_RS27815"
                     /old_locus_tag="FFY44_27815"
     CDS             32..211
                     /locus_tag="FFY44_RS27815"
                     /old_locus_tag="FFY44_27815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_021515244.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGKE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(239..769)
                     /locus_tag="FFY44_RS27820"
                     /old_locus_tag="FFY44_27820"
     CDS             complement(239..769)
                     /locus_tag="FFY44_RS27820"
                     /old_locus_tag="FFY44_27820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938511.1"
                     /translation="MALYMVFIEDAEDRDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERDKPAGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQISAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(776..1507)
                     /gene="mobC"
                     /locus_tag="FFY44_RS27825"
                     /old_locus_tag="FFY44_27825"
     CDS             complement(776..1507)
                     /gene="mobC"
                     /locus_tag="FFY44_RS27825"
                     /old_locus_tag="FFY44_27825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071653262.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGASGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(1507..3468)
                     /locus_tag="FFY44_RS27830"
                     /old_locus_tag="FFY44_27830"
     CDS             complement(1507..3468)
                     /locus_tag="FFY44_RS27830"
                     /old_locus_tag="FFY44_27830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_063938523.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIVWCAGGLV
                     AGILLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMGTNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEY
                     QPEAGRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(5054..7243)
                     /locus_tag="FFY44_RS27850"
                     /old_locus_tag="FFY44_27850"
     CDS             complement(5054..7243)
                     /locus_tag="FFY44_RS27850"
                     /old_locus_tag="FFY44_27850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_function="GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="WP_063938521.1"
                     /translation="MNKYLGLSIVYMMYTSSVMADDSGAVSKDKNISPEETLIVHGTAV
                     SESTTEGTDSYTTQSANTASKMNLSFRDTPQSTTVITRRRMDDVAAKNIIDVVKTAPGL
                     YVSTAEGSGRPNFMSRGFNANVMYEGFSLPWNSYIPSSQTSSAIFDRVEIVRGATGLVQ
                     GAGDPSAAINMIHKRPTRQYQASTNIGAGRWNDYRGGVDVSGPLNDSATLRGRAVVDGE
                     NSETFRDSEKRRNNVIYGIMEADLTDNTLLTIGGYQQYEYNNHWWYDLPISGTGHHLNL
                     PRSTFTGNDWEFERSKSKTAFATLEQKFSNSWKLKFSTLQNWRNADLLGTATYRHKDTR
                     QSMYQQVWGSLREYNSKSYDLSLNGDYALFGRVHQFVAGTTQQKYTDKDTGYTYSPIYI
                     ENVNIFDHGSHSSKKPMKMSGDASYDVTEQKSAYAATHFLLSDNWKILLGGRLDWYEYT
                     SKGTSINTGYKVTRNITKYAGSTYNLDDHHTIYGSYTDIFNPQSSTDLNGKVIKPIVGK
                     NYETGIKGEYFNGLLNASLAVFQVDQENRARLLPDQTGCVTNGNPQCYEASGKVRTKGI
                     DFELQGMVTPLWEIGVGYTYADTRYIKDGDRSNEGKRFNTAVSPQNLFKISSMYTFDGK
                     LTNWRAGVNTYWQSKIYANITDTRDGNPVINKQKAYSTTDIIIGYTPMPQLDLQLNITN
                     VFDKVYYTSIGESIGYGTETYGEPRNVKISATYRF"
                     /name="TonB-dependent siderophore receptor"
     gene            complement(7240..8325)
                     /locus_tag="FFY44_RS27855"
                     /old_locus_tag="FFY44_27855"
     CDS             complement(7240..8325)
                     /locus_tag="FFY44_RS27855"
                     /old_locus_tag="FFY44_27855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006327147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="WP_063938519.1"
                     /translation="MAITIKSAVNVRSRVLIYSLLLFTGSAYAADIAESTFLTRDIGNG
                     LYEMSFDGKNNSLWAASSSSFDRDKGHGIVYQLEPSTLKTKSAVKIARLGFATALDEEH
                     ERLYIGNTLDGSVTVIDTLTGKELSVIPLTEGQDEAHFRPTREMVIDKKNQRLYVSSAA
                     DKGALWVIDMAVGKKIKTIDGLGHAVTGIALNKNGDNIYLVNGDGEIINLDASSYEIKK
                     RFVVEPEKKHFFLNVSLDEKNGRAFITDPDIPGVLVVDVNSGKILHRIDVINSLAILFN
                     PLRNEIYITHRNAKQVSIVDSNSYYVKTTIKTRLMPNSLSLSHDGENLYVSVKQEKKEM
                     STMKDYILKIELSKIAMEPEE"
                     /name="YncE family protein"
     gene            8702..8965
                     /locus_tag="FFY44_RS27860"
                     /old_locus_tag="FFY44_27860"
     CDS             8702..8965
                     /locus_tag="FFY44_RS27860"
                     /old_locus_tag="FFY44_27860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938517.1"
                     /translation="MSNSTHYENANFLRELAERLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            8955..9254
                     /locus_tag="FFY44_RS27865"
                     /old_locus_tag="FFY44_27865"
     CDS             8955..9254
                     /locus_tag="FFY44_RS27865"
                     /old_locus_tag="FFY44_27865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009365129.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_063938515.1"
                     /translation="MPYEVEWKQGAIEDVTALFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHTVR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(9316..>9570)
                     /locus_tag="FFY44_RS30205"
                     /old_locus_tag="FFY44_27870"
                     /pseudo=""
     CDS             complement(9316..>9570)
                     /locus_tag="FFY44_RS30205"
                     /old_locus_tag="FFY44_27870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /name="entry exclusion protein 1"
     Typing          4269..4849
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.625"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(780..1507)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.885"
                     /coverage="100.0"
                     /evalue="3.84e-144"
ORIGIN
        1 gaagcacacc acacccggaa ggacagaaaa catgactaag caggaaaaaa ctgccctcaa
       61 tatggctaaa tttattaagg accagtcgct gctgttactc gagaaactga acgagctgga
      121 tatggatacc ccggccgaca tgtgcgagcg gctgcacgag gatgcggaaa cactctggca
      181 ggtgatgcgg gataagctcg ggaaggaata acagcaccgg catggccggt gctgccgttt
      241 attttttgcc gaacggccac caccggcggg cgggtttgtc ctccggcagt tcaggcaccg
      301 gcacgttaaa atagcggctg ttatattccc gcatcagctc cgctttcagc ttctcgttca
      361 ccgcgctttc cccgtgccgc tcgctgacca cctcaatcat cgcgctaatc tgctccggtg
      421 agcacttccc ggcatcaacc acctgcacac cgttctcgat ggcgaaccgc tccaccttgc
      481 gatagaggta atcacacagc ttcattcctg cgggtttgtc ccgttcccat tgcggatttt
      541 taccggtgaa aaagtacgtg gctttttcgt cgctgttgag ccgctcgtca tacatcccga
      601 tggatttcat catcgccaga tgctgggccc ggtcgacaaa acagaaaaca tacgccccgg
      661 tctgccaggc gagcaccacc atatcttcaa gatgctcgct ggtcggcatg atgcagcgct
      721 cgccgtcccg gtcctccgcg tcttcaataa ataccatgta cagcgccata atccctcact
      781 ccgttgtcat aaatttaaag cacgcaaaca gccgctcccg gtccagctcc gccgttttgc
      841 gggcctcgcc ggtctgtgga ttcacgattt catccaccga caggatcgcc cgctgcaccc
      901 tgacctgcgt cgcccggtcc gggcagatgt accagatttc atcgatgccg ttcgctttgc
      961 gattgaacag atacccggac accacctcct gatagcgcct gcggctcttc acggtgcgct
     1021 ccacctcaaa cgccaccgtt ctgccgtcca gctccgccag cgcgtccggc accttcatcc
     1081 ccttcagggc catacggtgc aggtaccgcc agccggaggc cccccgggcc tccatggcga
     1141 gacgcaggga ctgcacggcc agccggtgcg gcagctgcgc cgccgacacc cttgacggct
     1201 gaaacggaat aacgtccgtc agcggttcgt cctcgtcgaa cgagaacgcc accccgtgcg
     1261 gcgtcagtcc ccacacgctg acctgacggc cgtcaaccgg gtatttcgcc cggaccagca
     1321 gccctttcgc caccaggctg tccaggaagc ggtaaaccgg ctgcaccgcc gcaatcccca
     1381 gccgtcgcgc aaccagctcc gccgtggaac acagctcttc ccgcagccag cgcagggtca
     1441 gccgggtttt ctccgcgttc cgcgcctgcc gcttcgcgtg gctgacgttg tatcgctcca
     1501 gtgccatcag tacagccccg caaaatcatc atcacccggc cgctgccgcc cggcctccgg
     1561 ctggtactcc gccagcgggt ccgttttcgc cagcaccggg aagcgctgca gcgggatttc
     1621 ccggcgttca acccgcagcg ggctgctgaa cgccatccgg gccacgccca gcccggtcat
     1681 caccgcaaac ccgtcaggca ggtgctgcag ggtgttcacg tcaaacagcg gccgcgcctc
     1741 cttctgaatg aagctgtccc cgctgatatg ctccacgttc cccgcctccc ggctggcccg
     1801 gcggcgctcc acgtccaccc ggatttcccc ggtctggccc gccgcccact gcgcggactc
     1861 ctggctggcc gcccggtaga accagcggat cggcgtgtta tccagcaccg tggttttcac
     1921 aaactcagcg ggcagatcct ggccgcactg cccgaagtcg cccagcgact ggtgcgcgag
     1981 cagcaggttg cagtcccgac tgcgcagcgt ccccagcgcg ttcagcacgt acttcgacag
     2041 caggtattta atctcatcga gcatgatgct cgcgtgcggc cacctccgga actcatcccg
     2101 cgcaatgatg atttgcgcgc agcgggcaaa cagcatcttc tgcacccgga tcaccgcctc
     2161 atcgtccatc gagccgatga cgtacaggca gccgccgccg ttgattatcc ccgccacgtc
     2221 gatgccgctg tccgtctgca ggacgctcag gtcggccacg ttctccagct tggtgatcag
     2281 ccccttcacg tcctttttca gggcctccgg cagcgcgtat gccgccgcca gcacctgctg
     2341 aatgtttgcc ccctgcggga actgctccgc aatcagcttc gccgctttct gctcgctgat
     2401 gcggtagtgg tccgccacgt cgccggtctc actcaggtta aacccggcaa ccagcagatt
     2461 cttcagggcg tactggtcga gatcgcgaaa cagattcagc tgcgccggtt tccccggccg
     2521 cagatccagc agggtgaagt ccgggcagtg cgcccggaag acgtgcggcg cccacgcgtc
     2581 ccctttgggg tcgaagtaca ccaccgcgtc cccgaaatcg cgtacgcagc gaatgagcgc
     2641 gttggtgccc atcacgctct tgccgctccc gggcaggccg aggatctgaa tattggtctt
     2701 gcgccaggta tcacgcggga ccagcaccgg ctggccctgc tcatccttac ccagaaacac
     2761 cgcctgctgt ttccgggcct gctctgtgga aaaatcggcc agcgcgttaa ccggtaccgg
     2821 ctccccgaaa tgcaggtggc ggacgtcggt catttctccg gtccgctggc tgctgcgccg
     2881 gctctcctgg ttcagtttcg cattcaccgc ctcgtcagcg gccgcaaaat gaccgtcaag
     2941 cagcagaaat gccagcagcc gcgccagaag gatgccggcg acaagccctc cggcacacca
     3001 gacaatcccg aacaggttag ccggctgcag caggtaataa tctgcgttcg cgtagaggtc
     3061 cggacgcagg acggtttctc cccagctctc gataaacacg gcgacaacca gcccccccag
     3121 aagggccaga ccggcagcgc cggtcagcca gtacgcgtag ttccagacat gccgccgtgc
     3181 cctgaccatc cgcccgggcg aatccagcga agccagcacc gaaaatggcg cagtccggtt
     3241 attatcgcgc ttatagcgat cggcatactc ccgtcgctcc ttatgactca gccccacaaa
     3301 acgcatccgt ccggcccaga gttcagccag accgaaaaac aggccgataa ccaggaaagc
     3361 cccggccaga ccgtaatact ccctgtacag ccagctgacc cacgggtaaa tctgccgggc
     3421 cagctccggg atatgccact gccaggcaag cgaatccgta ttaaacatgc aatctctctc
     3481 cttcagacga cgggtttacc ggcgtggtgt gcagcccctg ctgcacaagc cgcggacatg
     3541 ccccgaggca tggcggcggc ggtcacgtcc gtggtgattg cattcagctt tcaccacgga
     3601 ggccagcctc tttccgcccc aagcggaaaa ggcgcagccc cggaggaaca acgagcacgc
     3661 cccagcgtgt gagaggaaaa ggcggcaccc cagtgcggtc ttttccggtg ttccggcccc
     3721 cgtcagagtt cgccatagcg agcggctgac cacccgcaaa atcaggagca gctttgctgc
     3781 ttcgtcaggt gcgttcagca ccgtgggttt ttgtgggtgg tcaggcgtga gcctgacggg
     3841 ggccgggtga atttctggcg tggccggcgt ccagccgagc tgagccagaa agagggggaa
     3901 agaccgtgcg tcggtggcgt ccagccagcg taagcagggg atttcctcct gaagtcagcc
     3961 acgccccagc ggggatgacc tgcatttctc cgcaaggaga aaaaagcaac agagataggt
     4021 taacgtaaaa tattcagcag aaaaccgaca gacttcacct atctgaatga tttatcggca
     4081 gggaagggaa aggcggtaac acggtgcaat atgtcacatc cgcgcgtccg gtggccgtct
     4141 gcgggagggg ggatattgcg cctcctccct cactcagtcg ctacgctccg gccgtgagac
     4201 tgcggcgggc gctgctaact ctcgcaaagt tacccacaga ttccgtggat aagcagggga
     4261 ctaacatgtg aggcaaaacg gcagggccgc gccggtggcg tttttccata ggcttcgccc
     4321 tcctgccaga attcacataa acagacgctt tttcggtgca tctgtgggag ccgtgaggct
     4381 caaccatgaa tctgacagta cgggcgaaac ccgacaggac ttaaagatcc ccaccgtttc
     4441 cggcgggtcg ctccctcttg cgctctcctg ttccgaccct gccgtttacc ggatacctgt
     4501 tccgcctttc tcccttacgg gaagtgtggc gctttctcat agctcacaca ctggtatctc
     4561 ggctcggtgt aggtcgttcg ctccaagctg ggctgtaagc aagaactccc cgttcagccc
     4621 gactgctgcg ccttatccgg taactgttca cttgagtcca acccggaaag acacgcaaaa
     4681 acgccactgg cagcagccat tggtaactgg gagttcgcag aggatttgtt tagctaaaca
     4741 cgcggttgct cttgaagtgt gcgccaaagt ccggctacac tggaaggaca gatttggttg
     4801 ctgtgctctg cgaaagccag ttaccacggt taagcagttc cccaactgac ttaatcttcg
     4861 atcaaaccac ctccccaggt ggttttttcg tttacagggc aaaagattac gacgaaccat
     4921 ggaggatctc aagaagatcc tctacatgat ttgattaacc atcaactgaa gtggcaaaca
     4981 gggggttgta cgccggaacc acaaaagacg gatttatagg ggcaaatccg aggagaagac
     5041 ccaatccgca tggttagaat ctataggttg cactaatttt aacatttctt ggttctccat
     5101 atgtttccgt gccatatcca atcgattcac ctatggatgt ataataaacc ttatcaaaga
     5161 cattagttat attgagctgt aaatccagtt gtggcattgg agtatagcca ataataatgt
     5221 cagttgttga ataagccttt tgtttattaa taaccggatt gccatccctt gtgtctgtta
     5281 tgttagcata tattttactt tgccagtacg tgtttactcc tgctcgccag tttgttaatt
     5341 ttccgtcaaa ggtgtacatg ctcgaaatct taaataaatt ttgtggcgac acggcagtat
     5401 tgaatcgctt gccttcatta ctacgatctc catcttttat atatcgcgta tctgcatatg
     5461 tatatcccac cccaatttcc cagagtggcg taaccatgcc ctgaagttcg aaatctatcc
     5521 cttttgtgcg tactttaccg ctagcctcat agcattgagg attgccatta gtaacacacc
     5581 ctgtctgatc gggaagcagc ctggctcgat tttcctggtc tacctgaaat accgccagag
     5641 aggcattaag caagccgtta aaatactcac ccttaatacc tgtctcataa ttcttaccaa
     5701 caataggctt gatcacttta ccattgaggt cggtgcttga ctgtggattg aatatatcgg
     5761 tatagctccc ataaatggta tgatgatcgt caagattgta tgttgaacct gcatatttgg
     5821 tgatatttct ggtaacttta taacctgtat taatggatgt acctttactt gtatattcat
     5881 accagtcaag acggccaccc agcaatattt tccagttatc actaagtaga aaatgagttg
     5941 ctgcgtaggc gcttttttgt tcagtgacgt cgtaggatgc atcacctgac atcttcatgg
     6001 gttttttcga agaatgtgaa ccatgatcaa atatattaac attctcaata tatattgggg
     6061 aatatgtata acctgtatct ttatcggtgt atttttgctg agttgttcca gccacgaatt
     6121 gatgaacacg accaaataaa gcatagtccc cattaagcga caggtcatag cttttggaat
     6181 tatattccct cagtgatccc catacttgct gatacatact ttgtcgggta tctttatgcc
     6241 ggtatgttgc cgtccctaac aggtcagcat ttctccagtt ttgcagcgtt gaaaatttca
     6301 gcttccagct attactgaac ttctgctcaa gagttgcaaa tgcagtttta cttttacttc
     6361 tttcaaactc ccagtcattg cctgtaaacg tcgagcgagg aagattaagg tggtgtccgg
     6421 taccagaaat aggtaagtca taccaccaat gattgttata ttcatactgc tgatatcccc
     6481 caatcgtaag tagggtatta tcagtcagat ctgcctccat aattccgtaa atgacgttat
     6541 tcctgcgttt ctcactatcc ctgaaggtct cactgttttc gccatccaca actgcccttc
     6601 cgcgtaaggt cgcgctgtca ttcagtggtc ctgatacatc aaccccaccc cggtagtcat
     6661 tccaccgtcc ggcaccgatg ttagtgctgg cctgatattg ccgggtcgga cgcttgtgta
     6721 tcatatttat cgcggctgaa gggtctccgg caccctgaac aagacctgtt gccccgcgga
     6781 ctatctccac ccggtcaaaa atagctgaac tagtttgtga acttggaatg tatgaattcc
     6841 acggcaggct aaatccttca tacatgacat tagcattaaa tcctctggac ataaaattcg
     6901 gtcttccgga accttctgct gtactgacat ataatccagg agcggtttta accacatcaa
     6961 taatattttt tgctgctaca tcatccattc ttcgtcgcgt aatgactgta gttgactgag
     7021 gtgtatcccg gaatgacaga ttcatctttg atgctgtatt ggctgactgg gttgtgtatg
     7081 aatctgttcc ttctgttgtg ctttcagaaa cagcggtccc atgaacaatt aatgtttctt
     7141 ctggtgaaat atttttatct ttactaactg cgccagaatc atcagccata acagatgatg
     7201 tatacatcat atatactatg ctcagcccta aatatttatt cattcttctg gctccatagc
     7261 aattttagat aattcgattt tgagtatgta atccttcata gtactcatct cttttttttc
     7321 ttgttttaca ctaacgtaca gattttcacc gtcatgtgat aaagacagac tgtttggcat
     7381 caggcgggtt tttatcgttg tttttacgta ataagaatta ctgtccacaa tgctgacctg
     7441 ttttgcatta cggtgcgtga tatagatttc gttccgtaaa ggattaaata aaatcgccag
     7501 tgaatttata acatcaattc tgtgtagtat ttttcccgag tttacatcaa caaccagcac
     7561 cccgggaata tccgggtcgg taataaatgc acgtccgttt ttttcatcca gacttacatt
     7621 aaggaaaaag tgttttttct caggctctac aacaaaccgt ttttttattt catagctact
     7681 ggcatccagg ttaataatct caccatcccc gttaaccaga taaatattat ccccattttt
     7741 atttaacgca attccagtaa cagcgtgccc cagtccatca atggttttaa ttttcttccc
     7801 gacagccatg tcaataaccc acagagcccc tttatctgcg gcgctggaga cataaagacg
     7861 ctgatttttt ttgtcaatga ccatttctct ggtcggtctg aaatgagcct catcctgtcc
     7921 ctcagttaac ggaattactg aaagttcttt tccggttaga gtatcaatga ccgtcactga
     7981 gccgtccaga gtattgccga tataaagtcg ctcatgttcc tcatccagtg ctgtggcaaa
     8041 cccaagacgt gcgattttaa cggcagattt tgttttcaga gtggatggtt ctaactgata
     8101 aacaattccg tggcccttat ctctgtcgaa tgaagacgat gatgctgccc agagactatt
     8161 atttttgcca tcaaaagaca tttcatacag accattgccg atatcacgag tcaggaatgt
     8221 tgattcagct atatcagccg catacgctga accagtaaac agtagtaaag agtatattaa
     8281 tactctgcta cggacattaa cggcggattt aatcgtgatt gccatactta catttcctta
     8341 caataaaaga aacaagaata tagcaaaagt gataatgatt atcactacca ttaatcacat
     8401 gatttaccat aacgtcgcat tacgcgcacc gctcagacgg tctgagggca tttccggcgg
     8461 ctacgggata aaaccggcaa caaaccaccc ggaaaaccgc cggaaacgcg cctgataaat
     8521 tttaacccgc tgcatggcta tgcacgcaac tgcatgtcgt gcagtttgac gtaaaatggg
     8581 tgtcgtaagc ccttttttac cagctttcgt cttcatcagc ctgactgaca cgagcatatt
     8641 ctttcagacc gccccctgct aaactccttc atatcgacgt atcagcccgg gaggctccac
     8701 aatgagcaac agtacacact atgaaaacgc caatttcctg cgggaactgg ccgagagact
     8761 gccccgcata ctgcctgaag gtggcccgga caaagctgca ctgttgcagc gcctggctaa
     8821 cgaagaactg gcccaggcag aatatgaaga tcaggttcgc gctaaagtga ccgccgcccg
     8881 cgctgacacg cgcccgggca tgaccacgga acaactccgc cagagactgc atggccgcta
     8941 tcaggaacta cgcgatgccg tatgaggtgg aatggaagca gggagccatc gaggatgtga
     9001 cagcattgtt tgactacatt gcggaaaatt catccctgtg ggatgcgcag aacgttaccg
     9061 atcgagttct cgctgcagcg gacaaactgg ccgacttccc gcgcctatat gaagtggact
     9121 cacgctatgg cgaggacgtt cgccggatca gtctgatggg acagcatgtc ctctatgatg
     9181 ttgatgacca gaccaggaaa gtccgggttc tggccgtggt tggccagcgc cagaatcccc
     9241 ataccgttcg ctgataaccg gtgatggggg cgcaattcat tgcgtggccg tcgccggttg
     9301 gacaagcaca gcgcgtcagc gttttcccca ctgccaccat cggcgcgatg ttccggccgg
     9361 tgtctctgct gtctctggtt tgtgttccag cagtctcatg gcctgcattt cctgccgcag
     9421 tccggccacc tcctccctga gtgcttttat ctccgcgatc aacgcgtcgg aggccatttt
     9481 ttcagtctca tccgacagtc tcaacccgtc ccgctcgtga gtctcaatct gtcttaattc
     9541 accgtacacc cggataagtt cgctggtatc c
//
