LOCUS       NZ_CP040550             5596 bp    DNA     circular CON 08-JAN-2024
DEFINITION  Klebsiella pneumoniae strain CR-HvKP5 plasmid pCR-HvKP5-p5, complete
            sequence.
ACCESSION   NZ_CP040550
VERSION     NZ_CP040550.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN09487518
            Assembly: GCF_005854025.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Dong,N. and Chen,S.
  TITLE     Tracking microevolution events among ST11 carbapenemase-producing
            hypervirulent Klebsiella pneumoniae outbreak strain
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Dong,N. and Chen,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUN-2018) Department of Applied Biology and Chemical
            Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon
            000000, Hong Kong
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: v0.4.4 v. v0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50x
            Sequencing Technology             :: Illumina NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/08/2024 04:38:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,894
            CDSs (total)                      :: 5,785
            Genes (coding)                    :: 5,599
            CDSs (with protein)               :: 5,599
            Genes (RNA)                       :: 109
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 82 of 186
            Pseudo Genes (incomplete)         :: 118 of 186
            Pseudo Genes (internal stop)      :: 28 of 186
            Pseudo Genes (multiple problems)  :: 39 of 186
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP040550.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="CR-HvKP5"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pCR-HvKP5-p5"
                     /geo_loc_name="China"
                     /collection_date="2016"
     gene            529..687
                     /locus_tag="FFT49_RS31280"
     CDS             529..687
                     /locus_tag="FFT49_RS31280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            1045..1371
                     /locus_tag="FFT49_RS29955"
                     /old_locus_tag="FFT49_29955"
     CDS             1045..1371
                     /locus_tag="FFT49_RS29955"
                     /old_locus_tag="FFT49_29955"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            1397..1942
                     /locus_tag="FFT49_RS29960"
                     /old_locus_tag="FFT49_29960"
     CDS             1397..1942
                     /locus_tag="FFT49_RS29960"
                     /old_locus_tag="FFT49_29960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            2008..2358
                     /locus_tag="FFT49_RS29965"
                     /old_locus_tag="FFT49_29965"
     CDS             2008..2358
                     /locus_tag="FFT49_RS29965"
                     /old_locus_tag="FFT49_29965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(2428..2694)
                     /locus_tag="FFT49_RS29970"
                     /old_locus_tag="FFT49_29970"
     CDS             complement(2428..2694)
                     /locus_tag="FFT49_RS29970"
                     /old_locus_tag="FFT49_29970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2678..2902)
                     /locus_tag="FFT49_RS29975"
                     /old_locus_tag="FFT49_29975"
     CDS             complement(2678..2902)
                     /locus_tag="FFT49_RS29975"
                     /old_locus_tag="FFT49_29975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            complement(3569..3781)
                     /locus_tag="FFT49_RS29980"
                     /old_locus_tag="FFT49_29980"
     CDS             complement(3569..3781)
                     /locus_tag="FFT49_RS29980"
                     /old_locus_tag="FFT49_29980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3788..4297)
                     /locus_tag="FFT49_RS29985"
                     /old_locus_tag="FFT49_29985"
     CDS             complement(3788..4297)
                     /locus_tag="FFT49_RS29985"
                     /old_locus_tag="FFT49_29985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<4332..5036)
                     /locus_tag="FFT49_RS31285"
                     /old_locus_tag="FFT49_29990"
                     /pseudo=""
     CDS             complement(<4332..5036)
                     /locus_tag="FFT49_RS31285"
                     /old_locus_tag="FFT49_29990"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4975..5319)
                     /locus_tag="FFT49_RS29995"
                     /old_locus_tag="FFT49_29995"
     CDS             complement(4975..5319)
                     /locus_tag="FFT49_RS29995"
                     /old_locus_tag="FFT49_29995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(5309..5485)
                     /locus_tag="FFT49_RS30665"
     CDS             complement(5309..5485)
                     /locus_tag="FFT49_RS30665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     Typing          241..638
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          complement(3454..4985)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaccagtcac agcgtttacg ctgtgtatac tggcttacta tgttggcatg agagcacgat
       61 tcatgaaagt gcctcatgtg gcaggtgtga tatgtggctc aggagcgtcc gcaataccat
      121 gcacggacag atgaaatgac gcctcctcgc tcactcggta gctgcgctcc tgccgttcgg
      181 ctgcggcggg cgtgaccgac tcacggataa cgggataaag caaaggcgct caaatcagaa
      241 agggccggag aaaaggatgc ggcgttgccg tttttccata ggctccgcct cccctgacga
      301 gcatcacaga aatggacgct caagtcagag gaggacgaaa cccgacagga cttaaagatg
      361 ccggtgattt ctctccggcg gctccctcgt gcgctctcct gttccgaccc tgccgtttac
      421 cggatacctg ttccgccttt ttccctttcg ggaagcgtgg cgctttctca tagctcacgc
      481 tgctggtatc tcggctcggt ttaggtcgtt cgctccaagc tgggctgtat gcacgaaccc
      541 cccgttcagc ccgactgctg cgccctttcc ggtaactatc atcttgagtc caacccggac
      601 ggacacgcaa aaacgccact ggcagcagcc attggtaact ggatatatac gagagagata
      661 ttgagaggtc ttgaagtggg tgcctaactg cggctacact gaaagaacag ttttggtatc
      721 tgcggtctcg tatacccagt tacccggtta agcagttccc caactgactt aaccttcgat
      781 caaaccgcct ccccaggcgg ttttttcgtt tacagtgcaa gagattacgc gcagaaaaaa
      841 aggatctcaa gaagatcctt taaatgatcg tcagttctgt tatccccgga gctccatcca
      901 tcccacggaa aaacacccgc cactgatgcc ctcagcgcac tgcacatcat gtacaaacca
      961 ctccctccct ccttttttat tgcgtttctt aatgcgcaat ggtagctttg tgcgcaacaa
     1021 aaaacgcaat agcaaggggg ggtaatgaga gaaagaacaa atatcatgct agatatagga
     1081 ttaaagcaat ccttacaacg gctttcgaat aaaaccaaaa tgagtctaag tgatcttatt
     1141 aaccagagat tatcaggttc actttcagtt gaagaacgca ttggcgaaaa gcctgaatgg
     1201 atgagaaacc ttgatcccta tctcgccaat cacttttcaa atattgagtt accgctccct
     1261 tcaccattaa aagaatacca agaaaaaatg gatgagatag attcaattat taatgacatg
     1321 atggttctaa aggaatattt cagagctaag ttttacgacg aaataaaata ggcacgctat
     1381 acggaggcgg agaattatgt tttttaaacg gtatttaacg gagaaggaaa tatacaaggc
     1441 agcactccca attgttaggc ttcaagggag agttaacact tcattacgtt ttttatccaa
     1501 atactcagat gtaaacaaaa atggattaat tgcattgcat cttaatttat tacattttat
     1561 ctcatacaac atacacggtg tagagtctct gaaaatcact caagcaggca taaacaaagc
     1621 tgtacaacaa agccctcacg cagatgaaat aaaaaatatt gcgcaggcaa taggacgcaa
     1681 aggacctgag tacatcgaag gattaattag aaacccatgg gggaagaaaa atgataatcc
     1741 actaatattt ataatgagca ttgaagaaag tataaaagaa acccttaact caggttatat
     1801 gttatataga gtagctccca taactgaggg tgaagcagct gctttcatgg atgttcttaa
     1861 acaattatta ttatttgagg ataattttcg cctttttggt agagacttgg tttataaaat
     1921 agaaaaatta aaaatcagat aacaaagtat ggtgaacgaa aaatgaaaga gaggatagtg
     1981 aaatgtgtaa taccaagatt ctcaactatg tattggttaa ttgcgttcct agcatttata
     2041 ttccccggtc gatcttttgc tgacggttac accgcttact accgatgtaa tggtactcag
     2101 gaagcatctc ttactgttaa agataacaaa ctaagtatgg ttgtaggtct ttctaagttt
     2161 aataggcaac tggatactta tagattcaaa gaaaagggaa tcgaagtagt aatgcagaga
     2221 gctgcttctg atgataactc tgaggccata atgtctttta ttatgcctga cgttaacaat
     2281 atgaccttat tagttaaaaa taacggctac attatcgtta agacaacctg ctggttaact
     2341 aaaaccgaca aacagtagaa cacgtagtta gtcgagtatt taccataata cctgttgtcg
     2401 gcatcaaaaa agcccgcata gcgggcttca gtcgtaaact tcgcggcgat gtccaatcgt
     2461 ggcggcaagg ataacaagct cgtcatctcg gatctcacac agaacgcgat aatctcccac
     2521 ccgatagcgc cagaactcgc ccagctcacc tttgagcggc ttccctgact ggcgaggatc
     2581 ggcagcaact gcaatgcgta aactcataaa atccacaatc cgtcgtgcgt tctgtttgtc
     2641 catcttgcgt aacgatttga gcgcccgatc ggaatagtta atcgtccaga ccaagtgact
     2701 tcctcacgtc ttcagacgaa tgcactgctt catcaccacg gcgaacgcgt gcagcagttt
     2761 ctgctgaaag ataataatcc tccaggtctt ccagatgcgc cagtattgct tcccgcgcat
     2821 aaaacgtctt tgttctgccg gtttgcttcg ccagcgagtc cagacgggac tcaatctcat
     2881 cagataaccg gatagcaagc atataaacct cccgaacacc atttgctata catgtatatc
     2941 aacagtacca caatcaacct aaaagaacaa aaaataatct tgttagcccc tcttgataat
     3001 ccgtaacgcg gtcaatatat ctagcttgag ggctttcctc ttaactattg aaaggataac
     3061 gattatgggt cttaagccgg gtccgaaacg aattgccgat tcaacagggg aacctgataa
     3121 acgccagcgt gacaataaaa aaacgccggg aaatactgac aaattgaaac ccagcaaatc
     3181 ttcaaaaaaa taagtcagta gttaaaagcc ctccggggct tttttgtatc tgccgttcac
     3241 gctgattaaa cataacaccc gttgcccgca ccgccccgga cgggttcgcc gcgaacgggg
     3301 tggcagtcag gtaaaaccgg caacaaacca cccgataaac cgccccgttc gcgacagata
     3361 aattttacct ggctgcatag ccatgcagcc aggtgaatga catccggttt cagcgtaaat
     3421 gcccgatgag aaccctgttt tcctgcattt cagcccagcg acggaccgtc atagcttcgc
     3481 tgccgggaac gcgcttccgt cagttccttt tcgtgctgcc gttgctcgtg cgcccgttct
     3541 gcggccacgg ccatctcatg acgctgcact accggctcaa acgctgcagt tgcccgctca
     3601 agcgttccac ctgctgactc aagcgcgtca cgcgctcgct cagcgccgcg ttctcccgtt
     3661 gcataagccc tgacattttc cgccattccc cgaaggcgct ctcccactcg tccagccttt
     3721 tcgagtagtc ctgctgtagc tgctctaatg cgctcagcaa ctgtttttcc agctcggtca
     3781 tcggcactta ctccttcacc agtctcagcg cgttctgctt cgaccgcccg ttcccgaccg
     3841 tccagctctg cgtcctgtct acggccgtcc ccttcggcag caccagaaaa cgtccgttgc
     3901 tgtcttccag gtacgtcacg ccccaggtct tcgcgttcag cttcgacagc gctgcgttct
     3961 gccggtcgat ttcggccaga tttgacgcta tcaagctccc ctgataccac aacaccccgc
     4021 tcaggctggc gaacagcagt cctgacacca tcgtgatggt cagccatgtc cgcccgacca
     4081 tacgcatcac gctcaggcgg ttcgactgca tggccgcgct cataccctgc tcgtgggcgc
     4141 ggatggcggc gctgattctc ttcccgcttt cgctcagttc cgctctgaca gacttctcgt
     4201 gctcgctgaa tgcggctttc agcatttcgc cggtactctg ctgctgcgct ttcgatttct
     4261 gctctaagtc cttcgccagc gttaaaagac tgttcataga tggctccctt aagtcggatg
     4321 tttcgccctc cgtccgggtc ggcaatgctg atgctgctct ttgtggtgcg cacgacctca
     4381 aaacctgcgc tttccagcgc ctcagtgacg tcctgacgcg ttttaagctc ccctgacgag
     4441 gcgagggcca gtaagccccg cgtaatagcc tctgcggctt cctgcttcgt tttgggtaac
     4501 gaagatggcg tcaccagcgc gcgccggttc tccggtgcgt tcgggtcgtg cagccccagt
     4561 cgcccgttca ccacggtctg ccacgcatcg atgcgcggac ggtctgcccg gtcgtagtac
     4621 ggctggagac gcctgccggt cagcagttcc gtgttcggta ccagaaaatt cagttccagc
     4681 cgccccttgt cccggtgttc aacccacagc acgctgtact ggtctttatc gagtccgggc
     4741 atcagaaccc gctcgaagct cgccatcagc ttttcacgct ggccgggcgg taaatcctgt
     4801 tcagcaaaag acaggacgcc ggaggtgtac tttttggcgt agggcgaggc gtcgataagc
     4861 tcccggacct cctccggctt tccctgcagg acgatggccc cgtcgcgctg gcggtctttc
     4921 cccagcagat aatccaccgg cccggcaccg ccgcctcgcc ctcgcggatg aaacttaacg
     4981 atcatcgtct gccccctttt ccagcacggc atgccgcagc cgctcgagtt cggcatcaat
     5041 ggccatcagc gcggccacca cctgaacccg gtcagcgcct gaccactggc cgccgttaat
     5101 tttccgggcg atctggttga ggttgttccc catcccggca agctggcgca gcaaaaccgg
     5161 atcgatggac ggcagccgca acgaccgcgc cgggcgcgta tcgagacagg tttcccgcat
     5221 ccacaccgcc agttgccttc cattgcagcg ctccagcagc tgctggtact ccccatcggt
     5281 cacccggata cctaaaaaac gggtacgctt aaccgccatc ctctcctccg atatccgcaa
     5341 aatgtgaccg ggctgaacgc tcaagctctg ccatctgctc ccggcggtaa tcttcctcct
     5401 gtgcctgacg catcagattt gccatcactg ccggatgcca ggatcttttt tttacgacga
     5461 gcggcttctt cttcggcttc tgcattatcc ctccttcgca aacgccggac ggcacggaaa
     5521 agttttccgg gtcgctccgg cgttggacaa gcgccagcgc gtcagtggtg ttagcgggtt
     5581 tcggggcgca gccctg
//
