LOCUS       NZ_CP040549            11970 bp    DNA     circular CON 08-JAN-2024
DEFINITION  Klebsiella pneumoniae strain CR-HvKP5 plasmid pCR-HvKP5-p4, complete
            sequence.
ACCESSION   NZ_CP040549
VERSION     NZ_CP040549.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN09487518
            Assembly: GCF_005854025.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Dong,N. and Chen,S.
  TITLE     Tracking microevolution events among ST11 carbapenemase-producing
            hypervirulent Klebsiella pneumoniae outbreak strain
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   Dong,N. and Chen,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUN-2018) Department of Applied Biology and Chemical
            Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon
            000000, Hong Kong
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: v0.4.4 v. v0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50x
            Sequencing Technology             :: Illumina NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/08/2024 04:38:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,894
            CDSs (total)                      :: 5,785
            Genes (coding)                    :: 5,599
            CDSs (with protein)               :: 5,599
            Genes (RNA)                       :: 109
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 82 of 186
            Pseudo Genes (incomplete)         :: 118 of 186
            Pseudo Genes (internal stop)      :: 28 of 186
            Pseudo Genes (multiple problems)  :: 39 of 186
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP040549.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="CR-HvKP5"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pCR-HvKP5-p4"
                     /geo_loc_name="China"
                     /collection_date="2016"
     gene            95..1780
                     /locus_tag="FFT49_RS29870"
                     /old_locus_tag="FFT49_29870"
     CDS             95..1780
                     /locus_tag="FFT49_RS29870"
                     /old_locus_tag="FFT49_29870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            1790..2047
                     /locus_tag="FFT49_RS29875"
                     /old_locus_tag="FFT49_29875"
     CDS             1790..2047
                     /locus_tag="FFT49_RS29875"
                     /old_locus_tag="FFT49_29875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            2132..2281
                     /locus_tag="FFT49_RS29880"
                     /old_locus_tag="FFT49_29880"
     CDS             2132..2281
                     /locus_tag="FFT49_RS29880"
                     /old_locus_tag="FFT49_29880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            3765..5729
                     /locus_tag="FFT49_RS29890"
                     /old_locus_tag="FFT49_29890"
     CDS             3765..5729
                     /locus_tag="FFT49_RS29890"
                     /old_locus_tag="FFT49_29890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            5729..6460
                     /gene="mobC"
                     /locus_tag="FFT49_RS29895"
                     /old_locus_tag="FFT49_29895"
     CDS             5729..6460
                     /gene="mobC"
                     /locus_tag="FFT49_RS29895"
                     /old_locus_tag="FFT49_29895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            6467..6997
                     /locus_tag="FFT49_RS29900"
                     /old_locus_tag="FFT49_29900"
     CDS             6467..6997
                     /locus_tag="FFT49_RS29900"
                     /old_locus_tag="FFT49_29900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(7024..7203)
                     /locus_tag="FFT49_RS29905"
                     /old_locus_tag="FFT49_29905"
     CDS             complement(7024..7203)
                     /locus_tag="FFT49_RS29905"
                     /old_locus_tag="FFT49_29905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(7229..7657)
                     /locus_tag="FFT49_RS29910"
                     /old_locus_tag="FFT49_29910"
     CDS             complement(7229..7657)
                     /locus_tag="FFT49_RS29910"
                     /old_locus_tag="FFT49_29910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(7821..8135)
                     /locus_tag="FFT49_RS29915"
                     /old_locus_tag="FFT49_29915"
     CDS             complement(7821..8135)
                     /locus_tag="FFT49_RS29915"
                     /old_locus_tag="FFT49_29915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(8123..8401)
                     /locus_tag="FFT49_RS29920"
                     /old_locus_tag="FFT49_29920"
     CDS             complement(8123..8401)
                     /locus_tag="FFT49_RS29920"
                     /old_locus_tag="FFT49_29920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(8576..8941)
                     /locus_tag="FFT49_RS31040"
                     /old_locus_tag="FFT49_29925"
     CDS             complement(8576..8941)
                     /locus_tag="FFT49_RS31040"
                     /old_locus_tag="FFT49_29925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(8938..9309)
                     /gene="tolA"
                     /locus_tag="FFT49_RS29930"
                     /old_locus_tag="FFT49_29930"
     CDS             complement(8938..9309)
                     /gene="tolA"
                     /locus_tag="FFT49_RS29930"
                     /old_locus_tag="FFT49_29930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(9583..9828)
                     /locus_tag="FFT49_RS29935"
                     /old_locus_tag="FFT49_29935"
     CDS             complement(9583..9828)
                     /locus_tag="FFT49_RS29935"
                     /old_locus_tag="FFT49_29935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            10473..11960
                     /gene="ltrA"
                     /locus_tag="FFT49_RS29945"
                     /old_locus_tag="FFT49_29945"
     CDS             10473..11960
                     /gene="ltrA"
                     /locus_tag="FFT49_RS29945"
                     /old_locus_tag="FFT49_29945"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     BGC             1..6780
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(2385..2963)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5730..6457
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 ccgggatctg tgcggggggt atccggtaac gggtatccct accgcgacat gttggcttta
       61 atttattgtt ttaaatgtca aagaggaaaa cggtatgagt ggtggagacg gtcgaggtcc
      121 gggtaattca ggtctgggac ataatggtgg tcaggccagt gggaatgtga acggtacgtc
      181 tggtaaaggt ggcccttcat caggtggtgg tacggatcca aacagcgggc cgggctgggg
      241 tacgacgcat acgccgaatg gagatattca taactataat ccgggggagt ttggtaacgg
      301 cgggagtaaa cccggtggta atggtggtaa cagcggcaat catagcggta gctctggtgg
      361 cggacagtct tcggccactg cgatggcctt cggtctgcct gccctggcta ctccgggggc
      421 tgaaggactg gctttatcag tttccggcga tgcgttgtcg gccgccgttg ctgatgtgct
      481 ggctgccctg aaggggccgt ttaagtttgg tctgtggggg attgcgatct acggtgtgct
      541 gccttctgag attgcaaaag atgatccgaa aatgatgtca aaaattatga cgtcattacc
      601 ggccgatgcg gtgacggaga ctccggcaag tactttacca ctggaccagg cgacggttcg
      661 tgtcagacaa cgggttgtgg atgtggtgaa ggatgagcgg cagcatattg cggttgtcgc
      721 aggtcggcca atgagtgtcc ctgtggtgga tgcgaaaccg acaaaacgtc cgggggtatt
      781 cagtgtgtcg attccgggtc tcccgtctct gcaggtgagc gtaccgaaag gtgttccgac
      841 agcgaaagcc ccgccaaaag gcattgttgc tgaaaaaggt gattcacgtc cggctggttt
      901 tacagccggt ggtaactccc gtgaggccgt tattcgtttc ccgaaagaga ccggacagaa
      961 gccggtttat gtgtcggtga cagatgttct taccccggca caggtaaaac agcgtcagga
     1021 ggaagaaaag cgtcgccagc aggcatggga cgccgctcat ccggaagagg ggctgaaaag
     1081 agactatgat aaagcgaaag ctgagctgga tgccgaagat aaaaatattg cgaccttaaa
     1141 cagccgcatt gcatcgacag agaaggcgct ccccggtgca agggctgctg ttcaggaagc
     1201 cgataaaaag gtaaaagagg cagaggcaaa taaagatgat tttgtgactt ataacccgcc
     1261 tcatgaatat ggctccgggt ggcaggatca ggttcgctat cttgataagg atattcagaa
     1321 tcagaatgag aaattaaaag cggctcaggc atctttaaac gcaatgaatg aatccttatc
     1381 cagagataag gctgcactta ccggggcgat ggagagccgg aaacaaaagg agaaaaaagc
     1441 gaaggatgca gaaaataagt taaatgagga aaagaacaag cctcgcaagg ggactaaaga
     1501 ctacggccat gattatcatc cagtacctaa aacagaagac ataaagggac ttggagagtt
     1561 aaagcgaggt gatcctaaaa cacctaaaca aggtggtggt ggtaagcggg cccgatggta
     1621 tggcgataaa aaacgtaaaa tttatgaatg ggattcccag cacggtgagc ttgaagggta
     1681 tcgtgccagt gatggcgaac acctaggcgc atttgatccc aaaacgggta agcaggttaa
     1741 agggccagat ccaaaacgga acattaaaaa atatctttga gaggtaaata tggggcttaa
     1801 attacgtttg acatggtttg ataagaaaac tgaagagttt aaaggggaag agtattctaa
     1861 agactttggt gacgatggtt ctgtcattga aagcttggga atgcctttaa aggataatat
     1921 taataatggt gagtttgatt tgaagaaaga atgggttcct ttactacagc cattttttaa
     1981 aaatcaaata aaacctgatg tggataattg ttatatagct tttgattatc gggacggtaa
     2041 ctggtaacaa aaaattatca aggatgattt gaaatactga cttccggtgt tcagggatga
     2101 atgccggagt ctttaaatac agggaaagta tatgaaaaag gtaaagacta tttttctttt
     2161 tatattgatt gttgctggct ttcttctggc cgcatgtcag gcaaattata tccgggatgt
     2221 tcagggcgga acggtggcac catcgtcctc ctctgaactg acggggatcg cggttcagta
     2281 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctt ttgccctgta
     2341 aacgaaaaaa ccacctgggg aggtggtttg atcgaaggtt aagtcagttg gggaactgct
     2401 taaccgtggt aactggcttt cgcagagcac agcaaccaaa tctgtccttc cagtgtagcc
     2461 ggactttggc gcacacttca agagcaaccg cgtgtttagc taaacaaatc ctctgcgaac
     2521 tcccagttac caatggctgc tgccagtggc gttttgcgtg ctttcccggg ttggactcaa
     2581 gtgaacagtt accggataag gcgcagcagt cgggctgaac ggggagttct tgcttacagc
     2641 ccagcttgga gcgaacgacc tacaccgagc cgagatacca gtgtgtgagc tatgagaaag
     2701 cgccacactt cccgcaaggg agaacggcgg acaggtatcc ggtaaacggc agggtcggaa
     2761 caggagagcg caagagggag cgacctaccg gaaacggtgg ggatctttaa gtcctgtcgg
     2821 gtttcgcccg tactgtcaga ttcatggttg agcctcacgg ctcccacaga tgcaccgaaa
     2881 aagcgtctgt ttatgtgaac tctggcagga gggcggagcc tatggaaaaa cgccaccggc
     2941 gcggccctgc cgttttgcct cacatgttag tcccctgctt atccacggaa tctgtgggta
     3001 actttgtatg tgtccgcagc gcccgccgca gtctcacgcc cggagcgtag cgaccgagtg
     3061 agcgaggagg cgcaatttcc ccggcacctg caagacggtc accggacgcg ctgatgtggg
     3121 atattgtccc ctgattaagt ctttctattc cctgacgatc aattatcagg ataggtgaac
     3181 catgtcggtt tttcgctgaa cattttacgt taacctatct ctgttgcttt tttctccttg
     3241 cggagaaatg caagtcatcc ccgctggggc gtggctgact tcaggggaaa agaccttgct
     3301 tacgctggct ggacgccacc gacgcacggt cttttcccct ctttctggct ctgtccggct
     3361 ggacgccggc cacgccagaa attcacccgg cccccgtcag gctcacgcct gaccacccac
     3421 aaaaatccac ggtgctggac gcacctgacg aagcagcaaa gctgctcctg attttgcggg
     3481 tggtcagccg ctcgctatgg cgaactctga cgggggccgg aacaccggaa aagaccgcac
     3541 tggggtgccg ccttttcctc tcacacgctg gggcgtgctc gttgttcctc cggggctgcg
     3601 ccttttccgc atggggcgga aagaggctgg cctccgcggt gaaagctgaa cgcaatcacc
     3661 acggacgtga ccgccgccgc catgccttgg ggcatgtccg cggcttgtgc agcgggggct
     3721 gcgcatcacg ccggtaaacc cgtcgtctga aggagagaga ttgcatgttt aatacggatt
     3781 cgcttgcctg gcagtggcat atcccggagc tggcccggca gatttacccg tgggtcagct
     3841 ggctgtaccg ggagtattac agtctggccg gtttttttct ggttatcggc ctgtttttcg
     3901 gtctggctga actctgggcc ggccgaatgc gttttgtggg gctgagccat aaggcgcggc
     3961 gggagtatgc cgatcgctat aagcgcgata ataaccggac tgtgccattt tcggtgctgg
     4021 cctcgctgga ttcacccggg cggatggtca gggcacggcg acatgtctgg aactacgcgt
     4081 actggctgac cggcgctgcc ggtctggccc ttctgggggg gctggttgtc gccgtgttta
     4141 tcgagagctg gggagaaacc gtcctgcgcc cggacctcta tgcgaacgca gattattacc
     4201 tgctgcagcc ggctaacctg ttcgggatag cctggtgtgc cggagggctt gtcgccggcg
     4261 tccttctggc gcggctgctg gcgttcctgc tgcttgatgg tcattttgcg gccgctgacg
     4321 aggcggtgaa cgcgaaactg aatcaggaga gccggcgcag cagccagcgg accggagaaa
     4381 tgacagacgt ccgccacctg catttcgggg agccggtacc ggttaacgcg ctggccgatt
     4441 tttccacgga gcaggcccgg aaacagcagg cggtgtttct gggtaaggat gagcagggcc
     4501 agccggtgct ggtccctcgt gatacctggc gcaagaccaa tattcagatc ctcggcctgc
     4561 ccgggagcgg caagagcgcg atggccacca acgcgctcat tcgctgcgta cgcgatttcg
     4621 gggacgctgt ggtgtacttc gaccccaaag gggacgcgtg ggcgccgcac gtcttccggg
     4681 cgcactgccc ggacttcacc ctgctggatc tgcggccggg gaaaccggcg cagctgaatc
     4741 tgttccgcga tctcgaccag tacgccctga agaatctgct ggttgccggg tttaacctga
     4801 gtgagaccgg cgacgtggcg gaccactacc gcatcagcga gcagaaagcg gcgaagctga
     4861 ttgcggagca gttcccgcag ggggcaaaca ttcagcaggt gctggcggcg gcgtacgcgt
     4921 tgccggaggc cctgaaaaag gacgtgaagg ggctgatcac caagctggag aacgtggccg
     4981 acctgagcgt cctgcagacg gacagcggta tcgatgtggc ggggataatc aacggcggcg
     5041 gctgcctgta cgtcatcggc tcgatggacg atgaggcggt gatccgggtg cagaagatgc
     5101 tgtttgcccg ctgcgcgcaa atcatcattg cgcgggatga gttccggagg tggccgcacg
     5161 cgagcatcat gctcgatgag attaaatatc tgctgtcgaa gtacgtgctg aacgcgctgg
     5221 ggacgctgcg cagccgggac tgcaacctgc tgctcgcgca ccagtcgctg ggcgacttcg
     5281 gtcagtgcgg gcaggatctg cccgctgatt ttgtgaaaac cacggtactg gataacacgc
     5341 cgatccgctg gttctatcgc gcggccagcc aggagtccgc gcagtgggcg gcgggccaga
     5401 ccggggagat ccgggtggac gtggagcgcc gccgggccag ccgggaggcg gggaacgtgg
     5461 agcacatcag cggcgacagc ttcatccaga aggaggcgcg gccgctgttt gacgtgaaca
     5521 ccctgcaaca cctgcctgac gggttcgcgg tgatgaccgg gctgggcgtg gcgcggctgg
     5581 cgttcagcag cccgctgcgg gttgagcgcc gggaaatttc gctgaaaacc ttcccggtgc
     5641 tggcgaaaac ggacccgctg gcggagtacc tgcctccggc cgacggcggc ggaccaaaag
     5701 cggatgatga ttttgcgggg ctttactgat gacactggag cagtacaacg tcagccacgc
     5761 gaagcggcag gcgcggaacg cggagaaaac acggctgacc ctgcgctggc tgcgggaaga
     5821 gctgtgttcc acggcggagc tggttgcgcg acggctgggg attgcggcgg tacagccggt
     5881 ttaccgcttc ctggacagtc tggtggcgaa agggctgctg gtccgggcga aatacccggt
     5941 tgacggccgt caggtcagcg tgtgggggct gacgccgcac ggggtggcgt tctcgttcga
     6001 cgaggacgag ccgctgacgg acgttattcc gtttcagccg tcaagggtgt cggcggcgca
     6061 gctgccgcac cggctggccg tgcagtccct gcgcctctcc atggagggcc ggggggccac
     6121 cggctggcgg tacctgcacc gtatggccct gaaagggatg aaagtgccgg acgcgctggc
     6181 ggagctggac ggcagaacgg tggcatttga ggtagagcgc accgtcaaga gccgcaggcg
     6241 gtatcaggaa gtggtgtccg ggtatctgtt caaccgccgg gcgaacggca tcgatgaaat
     6301 ctggtacatc tgccctgacc ggccgacgct tgtccgggta cagcgggcga ttctggcggt
     6361 ggatgaaatc gtgaatccac agaccggcga ggcccgcaaa acggtggagc tggaccggga
     6421 gaggctgttc gcgtgcttta aatttatgac aacggagtga gggattatgg cgctgtacat
     6481 ggtatttatt gaggacgtgg aagacaaaga cggcgagcgc tgcattatgc caaccagcga
     6541 gcatctggaa gacatggtgg tgctcgcctg gcagaccggg gcgtatgttt tctgttttgt
     6601 cgaccgggcc cagcatctgg cgatgatgaa atccatcggg atgtatgacg agcggctcaa
     6661 cagcgacgaa aaagccacgt actttttcac cggtgaaaat ccgcaatggg aacgggacaa
     6721 acccacagga atgaagctgt gtgattacct ctatcgaaag gtggagcggt tcgccatcga
     6781 gaacggtgtg caggtggttg atgccgggaa gtgctcaccg gagcagatta acgcgatgat
     6841 tgaggtggtc agcgagcggc acggggaaag cgcggtgaac gagaagctga aagcggagct
     6901 gatgcgggaa tataacagcc gctattttaa cgtgccggtg cctgaactgc cggaggacaa
     6961 acccgcccgc cggtggtggc cgttcggcaa aaaataacgc cagcaccggc atggccggtg
     7021 ctgttattct tccccgagct tatcccgcat cacctgccag agtgtttccg catcctcgtg
     7081 aagccgttcg cacatgtcgg ccggggtatc catgtccagc tcgttcagct tttccagcaa
     7141 cagcagcgac tgatccttaa tgaatttagc catattgagg gcagtttttt cctgcttcgt
     7201 catgttctcc attcccttcg gtaagttctc agctaaacca gccggaccag aagcgttttt
     7261 tcttctgtgt cagttctgtt gtcaatctgt ccacttcggc ctggagtttc cggcgctcat
     7321 catcgtgctg gcggcgttct tcttcccggg atgttttatc ttccagcatc agggtgacag
     7381 cctgccggag atcggccagt tccttttgca ttgccagaag ctgttcctgt gggaaatttt
     7441 tttgtgtatt ttcacggtgt gaaatgccgg gaatttgttt ctcagggata ttcacatttc
     7501 cgtatacacg gatcagttct gacacatcca cgacagggtt gtttttcccg tcacgtgaca
     7561 cggtaacctt tccctgtttt acatgattgt atagcgttct tctggtaatg cccgcggctt
     7621 gtgctgcctg tgaaaggttg agcaatgttt tcgccactgt atacccctga ttttatcggg
     7681 tgtgaaaggt gtgtaatttc tcacggtaaa tgtatcgctg aattttaccc gtttttccgt
     7741 tcaggatgtg gttttctgac gcgctgcgct tgtcaaaccg gcgacggcca cgcaatgaat
     7801 tgcgcccccg tcatcggtta ttagaggctg tctgcccccg gatacccgga ttctttctga
     7861 tgcctgacgg tgacgatcac aatctggtcc atttcccggt cgtggcggta gagcatcaca
     7921 tagccactgt cgccaaaccc gatcaccagt tccttatatt ccagcggcag aaacggcacc
     7981 gggcggccaa tgtctggcaa tgttttcagt tgctggatag cccggacgat cacctcacct
     8041 ccttttctgg ctgccagccg atttttggtt ttgagaaagt cctgaaggcg ttgtaaatcg
     8101 cgctgtgcca gcgcggaaat cgttacctgt ggcatggcgg agcatcctgc tcgttctcag
     8161 ttccccaggt acttatccag gcttccgctt cttccgcagt aaggtgcaga cccgtttcct
     8221 gatagtgctg ccatgcggcc tgtccgtccc gcaggtactg gctgcgcttc tcttcccggt
     8281 cgatgtattc ggtgattgcc tccagcatta gcgcgtgcgc tgagcggtgc cggtcatcgg
     8341 ccagcgtttt cagccggtcc ttaagttctt catcgagcct gatggatgtt gctgatgaca
     8401 taaaagcccc tttgtagtca tatgtattac acatgactac tttaatccgt ggcggcgcta
     8461 tttgccagaa atacggcttt tgctgcccat ttttcagctt cgcttccact aatcagggct
     8521 gctacaggct cactcatcat ccaggactat tgcagcctgc gggttaaagg acctgttact
     8581 ggcggcaacc atcatcattg agcttttggg tggtgactgg tcgcggatcc tgtaagccgt
     8641 catttttcag actgaattca aaatttataa cctgtcgcat tgccgggtgc cctgcgggat
     8701 atagccagtg cctcattgcc gtcagcgttt cccgaaaaaa aaggtgacag ccggtaagcg
     8761 acacgttgtc cactcgtcca tcgtccagaa tatcaaacgt ggcggtcacc tgaccttcga
     8821 tgcgttttgc tgcggcctgt accgggtagg ttggctgcag tgtggttacg ggttttagtg
     8881 gtatcgcatg taccggagtg aggaacatgg ccgccagcac tgtcagagta aaagctttca
     8941 tggtctgaaa tccagagggg cgtttttgaa tatttgccag gtattttcat ccggtgccgg
     9001 cggaattttg gcgtgggtaa cggctgatat tgctgcttta cagagtttcg gatctcctcc
     9061 ttcagcaatg gcgctgttca gagtgccatc acgttgtatg gatatccgca cggtgcacat
     9121 ttttcccgca taggcctctg gttcaaacat ctgcatttca acagcggtct tgatggccgc
     9181 agcgtagcta ctgattttag atgacttatc cagagtataa attttcggcg gctgggtggt
     9241 ggaatgctta caggccgtca ggagtaaggg gctgcacaat gccagcagga taaagggtat
     9301 taaacgcatc gggttgatga cctgtcagta aaaaatgtta catgtatatt tgtcactctt
     9361 tacggccacg ttcatttaca taaacacggc ttatcgtatc gtttttacac caaacggccc
     9421 gccatgcagt tgcgtgcata ggcatgcagc gggttacaat ttaccgggcg cgtttccggc
     9481 ggttttccgg gtggtttgtt gcctgtttta tcccgtagcc gccggaaacg ccctcagacc
     9541 gtctgagcgg tgcgggcaat gcgacgttat ggtaaatacc ccttaccggc ggcgctgtgg
     9601 ctgccaggtg aaggtggttt ccgtagcctc gtatttctcc cgacgcattt cctgcgcccg
     9661 cctggcttca cgttcgtcct gaatggcacg aagcaccctt tcccgcacct gttgttttac
     9721 cgcctttggc agcgttttgt tccctttcag gcccatggcc agtgcctgag cgacaaaggc
     9781 gtccgccagg cgcttttctt cagcgggagt cagtttcagg tgcgtcatag tggcctccgg
     9841 gcgtctcagg tttcccgaac agtttaaccg caggtcctct cctcatgcca gttagctttc
     9901 agatgtgtct ataccatctg aaatgtaagt gtgatgaatg tcttaaataa taaaatttgt
     9961 tgccatgaga aattttcggg aaaagacgcg atctgcttac aggtggctcc gggcgctttt
    10021 ttgtgcctga agatgagaac taatctcata tattgacacc tgaaaacggg aggagtaagg
    10081 taataattat actgtgtata tgtacagtgt gcgccgggag accggttaga gatcaagggg
    10141 tgaaagtccc cgaccattga aggaccagca atccacaagg tccccgagtc atgcgttgca
    10201 taccgcgagg tatggggcga agcgttgaca ggggtgttga caggccagcc attgagccac
    10261 gaaatgtata ttaaattacc gggtgccgac gttgtactgt taacggaagg caacatcata
    10321 gggtgcgata ctgcgagtgc cacatggacc cggcggggtc tgagaccctg gcatgtcaat
    10381 acgatctcta cgcgggaacc gggagatctc ccctctgacc atctgccagt gtcggagatg
    10441 gcccgcaccg ggaagacgag gagtcatagc cggtgatgta cggagaggag aagtcggact
    10501 cgctcatagt agcggcgaag caggcgaaca acccgaaagg agcggagtca gtggagcgaa
    10561 ggagcggggc caaggggaac gcggaacagc cacacatgcg ccggacacag agccgggaaa
    10621 gcatgtcaca gaggctgtca cgcgtgcggg aagctgcgaa gcagcggaag aaagaacggt
    10681 ttacagcatt gttccacctg ctgacagtcg aagcactgga agccgcattc ctctccctga
    10741 gcaggaaagc ggccgccgga gtggatggca tcaggtggat ggactacgcc ggaaacatga
    10801 agaacaacat aacagatctg caccggaggc tacatcaggg cagctacagg gcgcagcccg
    10861 gcaggcgtca ctacatccca aaagcggatg gaaaacaacg cccgctcggc atcgcctcgc
    10921 tggaggacaa gatcgtccag tatgcgctgg tgaaaatcct gaacgcagtc tatgaaaacg
    10981 actttatggg gttctcatac gggttcagac ccgggcgaag ccagcacgat gcactggacg
    11041 cactggccac agggctggta cgcactaacg taaactgggt actggatgcc gacatcagtc
    11101 agttcttcga cagggtgagc cacgaatggc tgatcaggtt cacagagcat cggatcggcg
    11161 accggagggt aatcaggctc atacgtaagt ggctcacagc cgggacgtcg gaggagggtc
    11221 aatggcgagc aacggaggaa ggcaccccac agggtgcggt catctcaccg ctgctggcaa
    11281 acatatacct ccactacgtc ttcgatctgt gggcgcatca gtggcgacgt cgctatgcca
    11341 caggcaatgt ggtaatggtc agatacgccg atgacatcgt catcgggttc gacaaacgat
    11401 acgatgcccg gcgcttccgt atagccatgc agcgcagact gagggagttc ggactcacgg
    11461 ttcacccgga gaaaacccgt ctgatggagt tcggccgctt cgctgccgaa aaccgtgcca
    11521 tcaggggaaa aggcaaacca gaaacgttca acttcctcgg gttcacgcac atcagcggga
    11581 aagatcgcaa cggcaggttc atgctgatac gaaagacccg ccgggatcgg atgacggcaa
    11641 ctctgaaagc catcaaagac ggtctgcgaa ggcgctggca ttactcaatc cccgaacagg
    11701 gaaaatggct caggagagtg gttcagggat acctgaacta tcactcggta ccgggcaact
    11761 tccccaccat gcagaagttc aggacacacg taacaaacct ctggcgccgg gcgctcaggc
    11821 gcaggagcca gaaggatgat acgacctgga cgaaagcaaa caaactggca gccgcatggc
    11881 taccaagggt tcgggttctt catccatggc ctgtggagcg gttcaccgcc agacacccga
    11941 ggcaggagcc cggtgcgtaa atcgcgcacg
//
