LOCUS       NZ_CP040544             5596 bp    DNA     circular CON 08-JAN-2024
DEFINITION  Klebsiella pneumoniae strain CR-HvKP4 plasmid pCR-HvKP4-p5, complete
            sequence.
ACCESSION   NZ_CP040544
VERSION     NZ_CP040544.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN09487517
            Assembly: GCF_005854245.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Dong,N. and Chen,S.
  TITLE     Tracking microevolution events among ST11 carbapenemase-producing
            hypervirulent Klebsiella pneumoniae outbreak strain
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Dong,N. and Chen,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUN-2018) Department of Applied Biology and Chemical
            Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon
            000000, Hong Kong
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: v0.4.4 v. v0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50x
            Sequencing Technology             :: Illumina NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/08/2024 04:37:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,884
            CDSs (total)                      :: 5,769
            Genes (coding)                    :: 5,590
            CDSs (with protein)               :: 5,590
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 104 of 179
            Pseudo Genes (internal stop)      :: 28 of 179
            Pseudo Genes (multiple problems)  :: 36 of 179
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP040544.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="CR-HvKP4"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pCR-HvKP4-p5"
                     /geo_loc_name="China"
                     /collection_date="2016"
     gene            join(5379..5596,1..127)
                     /locus_tag="FFT48_RS29905"
                     /old_locus_tag="FFT48_29900"
     CDS             join(5379..5596,1..127)
                     /locus_tag="FFT48_RS29905"
                     /old_locus_tag="FFT48_29900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            336..>770
                     /locus_tag="FFT48_RS31085"
                     /old_locus_tag="FFT48_29905"
                     /pseudo=""
     CDS             336..>770
                     /locus_tag="FFT48_RS31085"
                     /old_locus_tag="FFT48_29905"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            805..1314
                     /locus_tag="FFT48_RS29915"
                     /old_locus_tag="FFT48_29910"
     CDS             805..1314
                     /locus_tag="FFT48_RS29915"
                     /old_locus_tag="FFT48_29910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            1321..1533
                     /locus_tag="FFT48_RS29920"
                     /old_locus_tag="FFT48_29915"
     CDS             1321..1533
                     /locus_tag="FFT48_RS29920"
                     /old_locus_tag="FFT48_29915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            2200..2424
                     /locus_tag="FFT48_RS29925"
                     /old_locus_tag="FFT48_29920"
     CDS             2200..2424
                     /locus_tag="FFT48_RS29925"
                     /old_locus_tag="FFT48_29920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            2408..2674
                     /locus_tag="FFT48_RS29930"
                     /old_locus_tag="FFT48_29925"
     CDS             2408..2674
                     /locus_tag="FFT48_RS29930"
                     /old_locus_tag="FFT48_29925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2744..3094)
                     /locus_tag="FFT48_RS29935"
                     /old_locus_tag="FFT48_29930"
     CDS             complement(2744..3094)
                     /locus_tag="FFT48_RS29935"
                     /old_locus_tag="FFT48_29930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(3160..3705)
                     /locus_tag="FFT48_RS29940"
                     /old_locus_tag="FFT48_29935"
     CDS             complement(3160..3705)
                     /locus_tag="FFT48_RS29940"
                     /old_locus_tag="FFT48_29935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(3731..4057)
                     /locus_tag="FFT48_RS29945"
                     /old_locus_tag="FFT48_29940"
     CDS             complement(3731..4057)
                     /locus_tag="FFT48_RS29945"
                     /old_locus_tag="FFT48_29940"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            complement(4415..4573)
                     /locus_tag="FFT48_RS31215"
     CDS             complement(4415..4573)
                     /locus_tag="FFT48_RS31215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            5213..5389
                     /locus_tag="FFT48_RS30635"
     CDS             5213..5389
                     /locus_tag="FFT48_RS30635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     Typing          5048..5127
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(4465..4862)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          118..1649
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aattaacggc ggccagtggt caggcgctga ccgggttcag gtggtggccg cgctgatggc
       61 cattgatgcc gaactcgagc ggctgcggca tgccgtgctg gaaaaggggg cagacgatga
      121 tcgttaagtt tcatccgcga gggcgaggcg gcggtgccgg gccggtggat tatctgctgg
      181 ggaaagaccg ccagcgcgac ggggccatcg tcctgcaggg aaagccggag gaggtccggg
      241 agcttatcga cgcctcgccc tacgccaaaa agtacacctc cggcgtcctg tcttttgctg
      301 aacaggattt accgcccggc cagcgtgaaa agctgatggc gagcttcgag cgggttctga
      361 tgcccggact cgataaagac cagtacagcg tgctgtgggt tgaacaccgg gacaaggggc
      421 ggctggaact gaattttctg gtaccgaaca cggaactgct gaccggcagg cgtctccagc
      481 cgtactacga ccgggcagac cgtccgcgca tcgatgcgtg gcagaccgtg gtgaacgggc
      541 gactggggct gcacgacccg aacgcaccgg agaaccggcg cgcgctggtg acgccatctt
      601 cgttacccaa aacgaagcag gaagccgcag aggctattac gcggggctta ctggccctcg
      661 cctcgtcagg ggagcttaaa acgcgtcagg acgtcactga ggcgctggaa agcgcaggtt
      721 ttgaggtcgt gcgcaccaca aagagcagca tcagcattgc cgacccggac ggagggcgaa
      781 acatccgact taagggagcc atctatgaac agtcttttaa cgctggcgaa ggacttagag
      841 cagaaatcga aagcgcagca gcagagtacc ggcgaaatgc tgaaagccgc attcagcgag
      901 cacgagaagt ctgtcagagc ggaactgagc gaaagcggga agagaatcag cgccgccatc
      961 cgcgcccacg agcagggtat gagcgcggcc atgcagtcga accgcctgag cgtgatgcgt
     1021 atggtcgggc ggacatggct gaccatcacg atggtgtcag gactgctgtt cgccagcctg
     1081 agcggggtgt tgtggtatca ggggagcttg atagcgtcaa atctggccga aatcgaccgg
     1141 cagaacgcag cgctgtcgaa gctgaacgcg aagacctggg gcgtgacgta cctggaagac
     1201 agcaacggac gttttctggt gctgccgaag gggacggccg tagacaggac gcagagctgg
     1261 acggtcggga acgggcggtc gaagcagaac gcgctgagac tggtgaagga gtaagtgccg
     1321 atgaccgagc tggaaaaaca gttgctgagc gcattagagc agctacagca ggactactcg
     1381 aaaaggctgg acgagtggga gagcgccttc ggggaatggc ggaaaatgtc agggcttatg
     1441 caacgggaga acgcggcgct gagcgagcgc gtgacgcgct tgagtcagca ggtggaacgc
     1501 ttgagcgggc aactgcagcg tttgagccgg tagtgcagcg tcatgagatg gccgtggccg
     1561 cagaacgggc gcacgagcaa cggcagcacg aaaaggaact gacggaagcg cgttcccggc
     1621 agcgaagcta tgacggtccg tcgctgggct gaaatgcagg aaaacagggt tctcatcggg
     1681 catttacgct gaaaccggat gtcattcacc tggctgcatg gctatgcagc caggtaaaat
     1741 ttatctgtcg cgaacggggc ggtttatcgg gtggtttgtt gccggtttta cctgactgcc
     1801 accccgttcg cggcgaaccc gtccggggcg gtgcgggcaa cgggtgttat gtttaatcag
     1861 cgtgaacggc agatacaaaa aagccccgga gggcttttaa ctactgactt atttttttga
     1921 agatttgctg ggtttcaatt tgtcagtatt tcccggcgtt tttttattgt cacgctggcg
     1981 tttatcaggt tcccctgttg aatcggcaat tcgtttcgga cccggcttaa gacccataat
     2041 cgttatcctt tcaatagtta agaggaaagc cctcaagcta gatatattga ccgcgttacg
     2101 gattatcaag aggggctaac aagattattt tttgttcttt taggttgatt gtggtactgt
     2161 tgatatacat gtatagcaaa tggtgttcgg gaggtttata tgcttgctat ccggttatct
     2221 gatgagattg agtcccgtct ggactcgctg gcgaagcaaa ccggcagaac aaagacgttt
     2281 tatgcgcggg aagcaatact ggcgcatctg gaagacctgg aggattatta tctttcagca
     2341 gaaactgctg cacgcgttcg ccgtggtgat gaagcagtgc attcgtctga agacgtgagg
     2401 aagtcacttg gtctggacga ttaactattc cgatcgggcg ctcaaatcgt tacgcaagat
     2461 ggacaaacag aacgcacgac ggattgtgga ttttatgagt ttacgcattg cagttgctgc
     2521 cgatcctcgc cagtcaggga agccgctcaa aggtgagctg ggcgagttct ggcgctatcg
     2581 ggtgggagat tatcgcgttc tgtgtgagat ccgagatgac gagcttgtta tccttgccgc
     2641 cacgattgga catcgccgcg aagtttacga ctgaagcccg ctatgcgggc ttttttgatg
     2701 ccgacaacag gtattatggt aaatactcga ctaactacgt gttctactgt ttgtcggttt
     2761 tagttaacca gcaggttgtc ttaacgataa tgtagccgtt atttttaact aataaggtca
     2821 tattgttaac gtcaggcata ataaaagaca ttatggcctc agagttatca tcagaagcag
     2881 ctctctgcat tactacttcg attccctttt ctttgaatct ataagtatcc agttgcctat
     2941 taaacttaga aagacctaca accatactta gtttgttatc tttaacagta agagatgctt
     3001 cctgagtacc attacatcgg tagtaagcgg tgtaaccgtc agcaaaagat cgaccgggga
     3061 atataaatgc taggaacgca attaaccaat acatagttga gaatcttggt attacacatt
     3121 tcactatcct ctctttcatt tttcgttcac catactttgt tatctgattt ttaatttttc
     3181 tattttataa accaagtctc taccaaaaag gcgaaaatta tcctcaaata ataataattg
     3241 tttaagaaca tccatgaaag cagctgcttc accctcagtt atgggagcta ctctatataa
     3301 catataacct gagttaaggg tttcttttat actttcttca atgctcatta taaatattag
     3361 tggattatca tttttcttcc cccatgggtt tctaattaat ccttcgatgt actcaggtcc
     3421 tttgcgtcct attgcctgcg caatattttt tatttcatct gcgtgagggc tttgttgtac
     3481 agctttgttt atgcctgctt gagtgatttt cagagactct acaccgtgta tgttgtatga
     3541 gataaaatgt aataaattaa gatgcaatgc aattaatcca tttttgttta catctgagta
     3601 tttggataaa aaacgtaatg aagtgttaac tctcccttga agcctaacaa ttgggagtgc
     3661 tgccttgtat atttccttct ccgttaaata ccgtttaaaa aacataattc tccgcctccg
     3721 tatagcgtgc ctattttatt tcgtcgtaaa acttagctct gaaatattcc tttagaacca
     3781 tcatgtcatt aataattgaa tctatctcat ccattttttc ttggtattct tttaatggtg
     3841 aagggagcgg taactcaata tttgaaaagt gattggcgag atagggatca aggtttctca
     3901 tccattcagg cttttcgcca atgcgttctt caactgaaag tgaacctgat aatctctggt
     3961 taataagatc acttagactc attttggttt tattcgaaag ccgttgtaag gattgcttta
     4021 atcctatatc tagcatgata tttgttcttt ctctcattac ccccccttgc tattgcgttt
     4081 tttgttgcgc acaaagctac cattgcgcat taagaaacgc aataaaaaag gagggaggga
     4141 gtggtttgta catgatgtgc agtgcgctga gggcatcagt ggcgggtgtt tttccgtggg
     4201 atggatggag ctccggggat aacagaactg acgatcattt aaaggatctt cttgagatcc
     4261 tttttttctg cgcgtaatct cttgcactgt aaacgaaaaa accgcctggg gaggcggttt
     4321 gatcgaaggt taagtcagtt ggggaactgc ttaaccgggt aactgggtat acgagaccgc
     4381 agataccaaa actgttcttt cagtgtagcc gcagttaggc acccacttca agacctctca
     4441 atatctctct cgtatatatc cagttaccaa tggctgctgc cagtggcgtt tttgcgtgtc
     4501 cgtccgggtt ggactcaaga tgatagttac cggaaagggc gcagcagtcg ggctgaacgg
     4561 ggggttcgtg catacagccc agcttggagc gaacgaccta aaccgagccg agataccagc
     4621 agcgtgagct atgagaaagc gccacgcttc ccgaaaggga aaaaggcgga acaggtatcc
     4681 ggtaaacggc agggtcggaa caggagagcg cacgagggag ccgccggaga gaaatcaccg
     4741 gcatctttaa gtcctgtcgg gtttcgtcct cctctgactt gagcgtccat ttctgtgatg
     4801 ctcgtcaggg gaggcggagc ctatggaaaa acggcaacgc cgcatccttt tctccggccc
     4861 tttctgattt gagcgccttt gctttatccc gttatccgtg agtcggtcac gcccgccgca
     4921 gccgaacggc aggagcgcag ctaccgagtg agcgaggagg cgtcatttca tctgtccgtg
     4981 catggtattg cggacgctcc tgagccacat atcacacctg ccacatgagg cactttcatg
     5041 aatcgtgctc tcatgccaac atagtaagcc agtatacaca gcgtaaacgc tgtgactggt
     5101 tcagggctgc gccccgaaac ccgctaacac cactgacgcg ctggcgcttg tccaacgccg
     5161 gagcgacccg gaaaactttt ccgtgccgtc cggcgtttgc gaaggaggga taatgcagaa
     5221 gccgaagaag aagccgctcg tcgtaaaaaa aagatcctgg catccggcag tgatggcaaa
     5281 tctgatgcgt caggcacagg aggaagatta ccgccgggag cagatggcag agcttgagcg
     5341 ttcagcccgg tcacattttg cggatatcgg aggagaggat ggcggttaag cgtacccgtt
     5401 ttttaggtat ccgggtgacc gatggggagt accagcagct gctggagcgc tgcaatggaa
     5461 ggcaactggc ggtgtggatg cgggaaacct gtctcgatac gcgcccggcg cggtcgttgc
     5521 ggctgccgtc catcgatccg gttttgctgc gccagcttgc cgggatgggg aacaacctca
     5581 accagatcgc ccggaa
//
