LOCUS       NZ_CP040543            11970 bp    DNA     circular CON 08-JAN-2024
DEFINITION  Klebsiella pneumoniae strain CR-HvKP4 plasmid pCR-HvKP4-p4, complete
            sequence.
ACCESSION   NZ_CP040543
VERSION     NZ_CP040543.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN09487517
            Assembly: GCF_005854245.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Dong,N. and Chen,S.
  TITLE     Tracking microevolution events among ST11 carbapenemase-producing
            hypervirulent Klebsiella pneumoniae outbreak strain
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   Dong,N. and Chen,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUN-2018) Department of Applied Biology and Chemical
            Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon
            000000, Hong Kong
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: v0.4.4 v. v0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50x
            Sequencing Technology             :: Illumina NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/08/2024 04:37:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,884
            CDSs (total)                      :: 5,769
            Genes (coding)                    :: 5,590
            CDSs (with protein)               :: 5,590
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 84 of 179
            Pseudo Genes (incomplete)         :: 104 of 179
            Pseudo Genes (internal stop)      :: 28 of 179
            Pseudo Genes (multiple problems)  :: 36 of 179
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP040543.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="CR-HvKP4"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pCR-HvKP4-p4"
                     /geo_loc_name="China"
                     /collection_date="2016"
     gene            84..1769
                     /locus_tag="FFT48_RS29825"
                     /old_locus_tag="FFT48_29820"
     CDS             84..1769
                     /locus_tag="FFT48_RS29825"
                     /old_locus_tag="FFT48_29820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            1779..2036
                     /locus_tag="FFT48_RS29830"
                     /old_locus_tag="FFT48_29825"
     CDS             1779..2036
                     /locus_tag="FFT48_RS29830"
                     /old_locus_tag="FFT48_29825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            2121..2270
                     /locus_tag="FFT48_RS29835"
                     /old_locus_tag="FFT48_29830"
     CDS             2121..2270
                     /locus_tag="FFT48_RS29835"
                     /old_locus_tag="FFT48_29830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            3754..5718
                     /locus_tag="FFT48_RS29845"
                     /old_locus_tag="FFT48_29840"
     CDS             3754..5718
                     /locus_tag="FFT48_RS29845"
                     /old_locus_tag="FFT48_29840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            5718..6449
                     /gene="mobC"
                     /locus_tag="FFT48_RS29850"
                     /old_locus_tag="FFT48_29845"
     CDS             5718..6449
                     /gene="mobC"
                     /locus_tag="FFT48_RS29850"
                     /old_locus_tag="FFT48_29845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            6456..6986
                     /locus_tag="FFT48_RS29855"
                     /old_locus_tag="FFT48_29850"
     CDS             6456..6986
                     /locus_tag="FFT48_RS29855"
                     /old_locus_tag="FFT48_29850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(7013..7192)
                     /locus_tag="FFT48_RS29860"
                     /old_locus_tag="FFT48_29855"
     CDS             complement(7013..7192)
                     /locus_tag="FFT48_RS29860"
                     /old_locus_tag="FFT48_29855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(7218..7646)
                     /locus_tag="FFT48_RS29865"
                     /old_locus_tag="FFT48_29860"
     CDS             complement(7218..7646)
                     /locus_tag="FFT48_RS29865"
                     /old_locus_tag="FFT48_29860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(7810..8124)
                     /locus_tag="FFT48_RS29870"
                     /old_locus_tag="FFT48_29865"
     CDS             complement(7810..8124)
                     /locus_tag="FFT48_RS29870"
                     /old_locus_tag="FFT48_29865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(8112..8390)
                     /locus_tag="FFT48_RS29875"
                     /old_locus_tag="FFT48_29870"
     CDS             complement(8112..8390)
                     /locus_tag="FFT48_RS29875"
                     /old_locus_tag="FFT48_29870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(8565..8930)
                     /locus_tag="FFT48_RS31005"
                     /old_locus_tag="FFT48_29875"
     CDS             complement(8565..8930)
                     /locus_tag="FFT48_RS31005"
                     /old_locus_tag="FFT48_29875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(8927..9298)
                     /gene="tolA"
                     /locus_tag="FFT48_RS29885"
                     /old_locus_tag="FFT48_29880"
     CDS             complement(8927..9298)
                     /gene="tolA"
                     /locus_tag="FFT48_RS29885"
                     /old_locus_tag="FFT48_29880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(9572..9817)
                     /locus_tag="FFT48_RS29890"
                     /old_locus_tag="FFT48_29885"
     CDS             complement(9572..9817)
                     /locus_tag="FFT48_RS29890"
                     /old_locus_tag="FFT48_29885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            10462..11949
                     /gene="ltrA"
                     /locus_tag="FFT48_RS29900"
                     /old_locus_tag="FFT48_29895"
     CDS             10462..11949
                     /gene="ltrA"
                     /locus_tag="FFT48_RS29900"
                     /old_locus_tag="FFT48_29895"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     BGC             1..6769
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(2374..2952)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5719..6446
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 gcggggggta tccggtaacg ggtatcccta ccgcgacatg ttggctttaa tttattgttt
       61 taaatgtcaa agaggaaaac ggtatgagtg gtggagacgg tcgaggtccg ggtaattcag
      121 gtctgggaca taatggtggt caggccagtg ggaatgtgaa cggtacgtct ggtaaaggtg
      181 gcccttcatc aggtggtggt acggatccaa acagcgggcc gggctggggt acgacgcata
      241 cgccgaatgg agatattcat aactataatc cgggggagtt tggtaacggc gggagtaaac
      301 ccggtggtaa tggtggtaac agcggcaatc atagcggtag ctctggtggc ggacagtctt
      361 cggccactgc gatggccttc ggtctgcctg ccctggctac tccgggggct gaaggactgg
      421 ctttatcagt ttccggcgat gcgttgtcgg ccgccgttgc tgatgtgctg gctgccctga
      481 aggggccgtt taagtttggt ctgtggggga ttgcgatcta cggtgtgctg ccttctgaga
      541 ttgcaaaaga tgatccgaaa atgatgtcaa aaattatgac gtcattaccg gccgatgcgg
      601 tgacggagac tccggcaagt actttaccac tggaccaggc gacggttcgt gtcagacaac
      661 gggttgtgga tgtggtgaag gatgagcggc agcatattgc ggttgtcgca ggtcggccaa
      721 tgagtgtccc tgtggtggat gcgaaaccga caaaacgtcc gggggtattc agtgtgtcga
      781 ttccgggtct cccgtctctg caggtgagcg taccgaaagg tgttccgaca gcgaaagccc
      841 cgccaaaagg cattgttgct gaaaaaggtg attcacgtcc ggctggtttt acagccggtg
      901 gtaactcccg tgaggccgtt attcgtttcc cgaaagagac cggacagaag ccggtttatg
      961 tgtcggtgac agatgttctt accccggcac aggtaaaaca gcgtcaggag gaagaaaagc
     1021 gtcgccagca ggcatgggac gccgctcatc cggaagaggg gctgaaaaga gactatgata
     1081 aagcgaaagc tgagctggat gccgaagata aaaatattgc gaccttaaac agccgcattg
     1141 catcgacaga gaaggcgctc cccggtgcaa gggctgctgt tcaggaagcc gataaaaagg
     1201 taaaagaggc agaggcaaat aaagatgatt ttgtgactta taacccgcct catgaatatg
     1261 gctccgggtg gcaggatcag gttcgctatc ttgataagga tattcagaat cagaatgaga
     1321 aattaaaagc ggctcaggca tctttaaacg caatgaatga atccttatcc agagataagg
     1381 ctgcacttac cggggcgatg gagagccgga aacaaaagga gaaaaaagcg aaggatgcag
     1441 aaaataagtt aaatgaggaa aagaacaagc ctcgcaaggg gactaaagac tacggccatg
     1501 attatcatcc agtacctaaa acagaagaca taaagggact tggagagtta aagcgaggtg
     1561 atcctaaaac acctaaacaa ggtggtggtg gtaagcgggc ccgatggtat ggcgataaaa
     1621 aacgtaaaat ttatgaatgg gattcccagc acggtgagct tgaagggtat cgtgccagtg
     1681 atggcgaaca cctaggcgca tttgatccca aaacgggtaa gcaggttaaa gggccagatc
     1741 caaaacggaa cattaaaaaa tatctttgag aggtaaatat ggggcttaaa ttacgtttga
     1801 catggtttga taagaaaact gaagagttta aaggggaaga gtattctaaa gactttggtg
     1861 acgatggttc tgtcattgaa agcttgggaa tgcctttaaa ggataatatt aataatggtg
     1921 agtttgattt gaagaaagaa tgggttcctt tactacagcc attttttaaa aatcaaataa
     1981 aacctgatgt ggataattgt tatatagctt ttgattatcg ggacggtaac tggtaacaaa
     2041 aaattatcaa ggatgatttg aaatactgac ttccggtgtt cagggatgaa tgccggagtc
     2101 tttaaataca gggaaagtat atgaaaaagg taaagactat ttttcttttt atattgattg
     2161 ttgctggctt tcttctggcc gcatgtcagg caaattatat ccgggatgtt cagggcggaa
     2221 cggtggcacc atcgtcctcc tctgaactga cggggatcgc ggttcagtag aaaagatcaa
     2281 aggatcttct tgagatcctt tttttctgcg cgtaatcttt tgccctgtaa acgaaaaaac
     2341 cacctgggga ggtggtttga tcgaaggtta agtcagttgg ggaactgctt aaccgtggta
     2401 actggctttc gcagagcaca gcaaccaaat ctgtccttcc agtgtagccg gactttggcg
     2461 cacacttcaa gagcaaccgc gtgtttagct aaacaaatcc tctgcgaact cccagttacc
     2521 aatggctgct gccagtggcg ttttgcgtgc tttcccgggt tggactcaag tgaacagtta
     2581 ccggataagg cgcagcagtc gggctgaacg gggagttctt gcttacagcc cagcttggag
     2641 cgaacgacct acaccgagcc gagataccag tgtgtgagct atgagaaagc gccacacttc
     2701 ccgcaaggga gaacggcgga caggtatccg gtaaacggca gggtcggaac aggagagcgc
     2761 aagagggagc gacctaccgg aaacggtggg gatctttaag tcctgtcggg tttcgcccgt
     2821 actgtcagat tcatggttga gcctcacggc tcccacagat gcaccgaaaa agcgtctgtt
     2881 tatgtgaact ctggcaggag ggcggagcct atggaaaaac gccaccggcg cggccctgcc
     2941 gttttgcctc acatgttagt cccctgctta tccacggaat ctgtgggtaa ctttgtatgt
     3001 gtccgcagcg cccgccgcag tctcacgccc ggagcgtagc gaccgagtga gcgaggaggc
     3061 gcaatttccc cggcacctgc aagacggtca ccggacgcgc tgatgtggga tattgtcccc
     3121 tgattaagtc tttctattcc ctgacgatca attatcagga taggtgaacc atgtcggttt
     3181 ttcgctgaac attttacgtt aacctatctc tgttgctttt ttctccttgc ggagaaatgc
     3241 aagtcatccc cgctggggcg tggctgactt caggggaaaa gaccttgctt acgctggctg
     3301 gacgccaccg acgcacggtc ttttcccctc tttctggctc tgtccggctg gacgccggcc
     3361 acgccagaaa ttcacccggc ccccgtcagg ctcacgcctg accacccaca aaaatccacg
     3421 gtgctggacg cacctgacga agcagcaaag ctgctcctga ttttgcgggt ggtcagccgc
     3481 tcgctatggc gaactctgac gggggccgga acaccggaaa agaccgcact ggggtgccgc
     3541 cttttcctct cacacgctgg ggcgtgctcg ttgttcctcc ggggctgcgc cttttccgca
     3601 tggggcggaa agaggctggc ctccgcggtg aaagctgaac gcaatcacca cggacgtgac
     3661 cgccgccgcc atgccttggg gcatgtccgc ggcttgtgca gcgggggctg cgcatcacgc
     3721 cggtaaaccc gtcgtctgaa ggagagagat tgcatgttta atacggattc gcttgcctgg
     3781 cagtggcata tcccggagct ggcccggcag atttacccgt gggtcagctg gctgtaccgg
     3841 gagtattaca gtctggccgg tttttttctg gttatcggcc tgtttttcgg tctggctgaa
     3901 ctctgggccg gccgaatgcg ttttgtgggg ctgagccata aggcgcggcg ggagtatgcc
     3961 gatcgctata agcgcgataa taaccggact gtgccatttt cggtgctggc ctcgctggat
     4021 tcacccgggc ggatggtcag ggcacggcga catgtctgga actacgcgta ctggctgacc
     4081 ggcgctgccg gtctggccct tctggggggg ctggttgtcg ccgtgtttat cgagagctgg
     4141 ggagaaaccg tcctgcgccc ggacctctat gcgaacgcag attattacct gctgcagccg
     4201 gctaacctgt tcgggatagc ctggtgtgcc ggagggcttg tcgccggcgt ccttctggcg
     4261 cggctgctgg cgttcctgct gcttgatggt cattttgcgg ccgctgacga ggcggtgaac
     4321 gcgaaactga atcaggagag ccggcgcagc agccagcgga ccggagaaat gacagacgtc
     4381 cgccacctgc atttcgggga gccggtaccg gttaacgcgc tggccgattt ttccacggag
     4441 caggcccgga aacagcaggc ggtgtttctg ggtaaggatg agcagggcca gccggtgctg
     4501 gtccctcgtg atacctggcg caagaccaat attcagatcc tcggcctgcc cgggagcggc
     4561 aagagcgcga tggccaccaa cgcgctcatt cgctgcgtac gcgatttcgg ggacgctgtg
     4621 gtgtacttcg accccaaagg ggacgcgtgg gcgccgcacg tcttccgggc gcactgcccg
     4681 gacttcaccc tgctggatct gcggccgggg aaaccggcgc agctgaatct gttccgcgat
     4741 ctcgaccagt acgccctgaa gaatctgctg gttgccgggt ttaacctgag tgagaccggc
     4801 gacgtggcgg accactaccg catcagcgag cagaaagcgg cgaagctgat tgcggagcag
     4861 ttcccgcagg gggcaaacat tcagcaggtg ctggcggcgg cgtacgcgtt gccggaggcc
     4921 ctgaaaaagg acgtgaaggg gctgatcacc aagctggaga acgtggccga cctgagcgtc
     4981 ctgcagacgg acagcggtat cgatgtggcg gggataatca acggcggcgg ctgcctgtac
     5041 gtcatcggct cgatggacga tgaggcggtg atccgggtgc agaagatgct gtttgcccgc
     5101 tgcgcgcaaa tcatcattgc gcgggatgag ttccggaggt ggccgcacgc gagcatcatg
     5161 ctcgatgaga ttaaatatct gctgtcgaag tacgtgctga acgcgctggg gacgctgcgc
     5221 agccgggact gcaacctgct gctcgcgcac cagtcgctgg gcgacttcgg tcagtgcggg
     5281 caggatctgc ccgctgattt tgtgaaaacc acggtactgg ataacacgcc gatccgctgg
     5341 ttctatcgcg cggccagcca ggagtccgcg cagtgggcgg cgggccagac cggggagatc
     5401 cgggtggacg tggagcgccg ccgggccagc cgggaggcgg ggaacgtgga gcacatcagc
     5461 ggcgacagct tcatccagaa ggaggcgcgg ccgctgtttg acgtgaacac cctgcaacac
     5521 ctgcctgacg ggttcgcggt gatgaccggg ctgggcgtgg cgcggctggc gttcagcagc
     5581 ccgctgcggg ttgagcgccg ggaaatttcg ctgaaaacct tcccggtgct ggcgaaaacg
     5641 gacccgctgg cggagtacct gcctccggcc gacggcggcg gaccaaaagc ggatgatgat
     5701 tttgcggggc tttactgatg acactggagc agtacaacgt cagccacgcg aagcggcagg
     5761 cgcggaacgc ggagaaaaca cggctgaccc tgcgctggct gcgggaagag ctgtgttcca
     5821 cggcggagct ggttgcgcga cggctgggga ttgcggcggt acagccggtt taccgcttcc
     5881 tggacagtct ggtggcgaaa gggctgctgg tccgggcgaa atacccggtt gacggccgtc
     5941 aggtcagcgt gtgggggctg acgccgcacg gggtggcgtt ctcgttcgac gaggacgagc
     6001 cgctgacgga cgttattccg tttcagccgt caagggtgtc ggcggcgcag ctgccgcacc
     6061 ggctggccgt gcagtccctg cgcctctcca tggagggccg gggggccacc ggctggcggt
     6121 acctgcaccg tatggccctg aaagggatga aagtgccgga cgcgctggcg gagctggacg
     6181 gcagaacggt ggcatttgag gtagagcgca ccgtcaagag ccgcaggcgg tatcaggaag
     6241 tggtgtccgg gtatctgttc aaccgccggg cgaacggcat cgatgaaatc tggtacatct
     6301 gccctgaccg gccgacgctt gtccgggtac agcgggcgat tctggcggtg gatgaaatcg
     6361 tgaatccaca gaccggcgag gcccgcaaaa cggtggagct ggaccgggag aggctgttcg
     6421 cgtgctttaa atttatgaca acggagtgag ggattatggc gctgtacatg gtatttattg
     6481 aggacgtgga agacaaagac ggcgagcgct gcattatgcc aaccagcgag catctggaag
     6541 acatggtggt gctcgcctgg cagaccgggg cgtatgtttt ctgttttgtc gaccgggccc
     6601 agcatctggc gatgatgaaa tccatcggga tgtatgacga gcggctcaac agcgacgaaa
     6661 aagccacgta ctttttcacc ggtgaaaatc cgcaatggga acgggacaaa cccacaggaa
     6721 tgaagctgtg tgattacctc tatcgaaagg tggagcggtt cgccatcgag aacggtgtgc
     6781 aggtggttga tgccgggaag tgctcaccgg agcagattaa cgcgatgatt gaggtggtca
     6841 gcgagcggca cggggaaagc gcggtgaacg agaagctgaa agcggagctg atgcgggaat
     6901 ataacagccg ctattttaac gtgccggtgc ctgaactgcc ggaggacaaa cccgcccgcc
     6961 ggtggtggcc gttcggcaaa aaataacgcc agcaccggca tggccggtgc tgttattctt
     7021 ccccgagctt atcccgcatc acctgccaga gtgtttccgc atcctcgtga agccgttcgc
     7081 acatgtcggc cggggtatcc atgtccagct cgttcagctt ttccagcaac agcagcgact
     7141 gatccttaat gaatttagcc atattgaggg cagttttttc ctgcttcgtc atgttctcca
     7201 ttcccttcgg taagttctca gctaaaccag ccggaccaga agcgtttttt cttctgtgtc
     7261 agttctgttg tcaatctgtc cacttcggcc tggagtttcc ggcgctcatc atcgtgctgg
     7321 cggcgttctt cttcccggga tgttttatct tccagcatca gggtgacagc ctgccggaga
     7381 tcggccagtt ccttttgcat tgccagaagc tgttcctgtg ggaaattttt ttgtgtattt
     7441 tcacggtgtg aaatgccggg aatttgtttc tcagggatat tcacatttcc gtatacacgg
     7501 atcagttctg acacatccac gacagggttg tttttcccgt cacgtgacac ggtaaccttt
     7561 ccctgtttta catgattgta tagcgttctt ctggtaatgc ccgcggcttg tgctgcctgt
     7621 gaaaggttga gcaatgtttt cgccactgta tacccctgat tttatcgggt gtgaaaggtg
     7681 tgtaatttct cacggtaaat gtatcgctga attttacccg tttttccgtt caggatgtgg
     7741 ttttctgacg cgctgcgctt gtcaaaccgg cgacggccac gcaatgaatt gcgcccccgt
     7801 catcggttat tagaggctgt ctgcccccgg atacccggat tctttctgat gcctgacggt
     7861 gacgatcaca atctggtcca tttcccggtc gtggcggtag agcatcacat agccactgtc
     7921 gccaaacccg atcaccagtt ccttatattc cagcggcaga aacggcaccg ggcggccaat
     7981 gtctggcaat gttttcagtt gctggatagc ccggacgatc acctcacctc cttttctggc
     8041 tgccagccga tttttggttt tgagaaagtc ctgaaggcgt tgtaaatcgc gctgtgccag
     8101 cgcggaaatc gttacctgtg gcatggcgga gcatcctgct cgttctcagt tccccaggta
     8161 cttatccagg cttccgcttc ttccgcagta aggtgcagac ccgtttcctg atagtgctgc
     8221 catgcggcct gtccgtcccg caggtactgg ctgcgcttct cttcccggtc gatgtattcg
     8281 gtgattgcct ccagcattag cgcgtgcgct gagcggtgcc ggtcatcggc cagcgttttc
     8341 agccggtcct taagttcttc atcgagcctg atggatgttg ctgatgacat aaaagcccct
     8401 ttgtagtcat atgtattaca catgactact ttaatccgtg gcggcgctat ttgccagaaa
     8461 tacggctttt gctgcccatt tttcagcttc gcttccacta atcagggctg ctacaggctc
     8521 actcatcatc caggactatt gcagcctgcg ggttaaagga cctgttactg gcggcaacca
     8581 tcatcattga gcttttgggt ggtgactggt cgcggatcct gtaagccgtc atttttcaga
     8641 ctgaattcaa aatttataac ctgtcgcatt gccgggtgcc ctgcgggata tagccagtgc
     8701 ctcattgccg tcagcgtttc ccgaaaaaaa aggtgacagc cggtaagcga cacgttgtcc
     8761 actcgtccat cgtccagaat atcaaacgtg gcggtcacct gaccttcgat gcgttttgct
     8821 gcggcctgta ccgggtaggt tggctgcagt gtggttacgg gttttagtgg tatcgcatgt
     8881 accggagtga ggaacatggc cgccagcact gtcagagtaa aagctttcat ggtctgaaat
     8941 ccagaggggc gtttttgaat atttgccagg tattttcatc cggtgccggc ggaattttgg
     9001 cgtgggtaac ggctgatatt gctgctttac agagtttcgg atctcctcct tcagcaatgg
     9061 cgctgttcag agtgccatca cgttgtatgg atatccgcac ggtgcacatt tttcccgcat
     9121 aggcctctgg ttcaaacatc tgcatttcaa cagcggtctt gatggccgca gcgtagctac
     9181 tgattttaga tgacttatcc agagtataaa ttttcggcgg ctgggtggtg gaatgcttac
     9241 aggccgtcag gagtaagggg ctgcacaatg ccagcaggat aaagggtatt aaacgcatcg
     9301 ggttgatgac ctgtcagtaa aaaatgttac atgtatattt gtcactcttt acggccacgt
     9361 tcatttacat aaacacggct tatcgtatcg tttttacacc aaacggcccg ccatgcagtt
     9421 gcgtgcatag gcatgcagcg ggttacaatt taccgggcgc gtttccggcg gttttccggg
     9481 tggtttgttg cctgttttat cccgtagccg ccggaaacgc cctcagaccg tctgagcggt
     9541 gcgggcaatg cgacgttatg gtaaataccc cttaccggcg gcgctgtggc tgccaggtga
     9601 aggtggtttc cgtagcctcg tatttctccc gacgcatttc ctgcgcccgc ctggcttcac
     9661 gttcgtcctg aatggcacga agcacccttt cccgcacctg ttgttttacc gcctttggca
     9721 gcgttttgtt ccctttcagg cccatggcca gtgcctgagc gacaaaggcg tccgccaggc
     9781 gcttttcttc agcgggagtc agtttcaggt gcgtcatagt ggcctccggg cgtctcaggt
     9841 ttcccgaaca gtttaaccgc aggtcctctc ctcatgccag ttagctttca gatgtgtcta
     9901 taccatctga aatgtaagtg tgatgaatgt cttaaataat aaaatttgtt gccatgagaa
     9961 attttcggga aaagacgcga tctgcttaca ggtggctccg ggcgcttttt tgtgcctgaa
    10021 gatgagaact aatctcatat attgacacct gaaaacggga ggagtaaggt aataattata
    10081 ctgtgtatat gtacagtgtg cgccgggaga ccggttagag atcaaggggt gaaagtcccc
    10141 gaccattgaa ggaccagcaa tccacaaggt ccccgagtca tgcgttgcat accgcgaggt
    10201 atggggcgaa gcgttgacag gggtgttgac aggccagcca ttgagccacg aaatgtatat
    10261 taaattaccg ggtgccgacg ttgtactgtt aacggaaggc aacatcatag ggtgcgatac
    10321 tgcgagtgcc acatggaccc ggcggggtct gagaccctgg catgtcaata cgatctctac
    10381 gcgggaaccg ggagatctcc cctctgacca tctgccagtg tcggagatgg cccgcaccgg
    10441 gaagacgagg agtcatagcc ggtgatgtac ggagaggaga agtcggactc gctcatagta
    10501 gcggcgaagc aggcgaacaa cccgaaagga gcggagtcag tggagcgaag gagcggggcc
    10561 aaggggaacg cggaacagcc acacatgcgc cggacacaga gccgggaaag catgtcacag
    10621 aggctgtcac gcgtgcggga agctgcgaag cagcggaaga aagaacggtt tacagcattg
    10681 ttccacctgc tgacagtcga agcactggaa gccgcattcc tctccctgag caggaaagcg
    10741 gccgccggag tggatggcat caggtggatg gactacgccg gaaacatgaa gaacaacata
    10801 acagatctgc accggaggct acatcagggc agctacaggg cgcagcccgg caggcgtcac
    10861 tacatcccaa aagcggatgg aaaacaacgc ccgctcggca tcgcctcgct ggaggacaag
    10921 atcgtccagt atgcgctggt gaaaatcctg aacgcagtct atgaaaacga ctttatgggg
    10981 ttctcatacg ggttcagacc cgggcgaagc cagcacgatg cactggacgc actggccaca
    11041 gggctggtac gcactaacgt aaactgggta ctggatgccg acatcagtca gttcttcgac
    11101 agggtgagcc acgaatggct gatcaggttc acagagcatc ggatcggcga ccggagggta
    11161 atcaggctca tacgtaagtg gctcacagcc gggacgtcgg aggagggtca atggcgagca
    11221 acggaggaag gcaccccaca gggtgcggtc atctcaccgc tgctggcaaa catatacctc
    11281 cactacgtct tcgatctgtg ggcgcatcag tggcgacgtc gctatgccac aggcaatgtg
    11341 gtaatggtca gatacgccga tgacatcgtc atcgggttcg acaaacgata cgatgcccgg
    11401 cgcttccgta tagccatgca gcgcagactg agggagttcg gactcacggt tcacccggag
    11461 aaaacccgtc tgatggagtt cggccgcttc gctgccgaaa accgtgccat caggggaaaa
    11521 ggcaaaccag aaacgttcaa cttcctcggg ttcacgcaca tcagcgggaa agatcgcaac
    11581 ggcaggttca tgctgatacg aaagacccgc cgggatcgga tgacggcaac tctgaaagcc
    11641 atcaaagacg gtctgcgaag gcgctggcat tactcaatcc ccgaacaggg aaaatggctc
    11701 aggagagtgg ttcagggata cctgaactat cactcggtac cgggcaactt ccccaccatg
    11761 cagaagttca ggacacacgt aacaaacctc tggcgccggg cgctcaggcg caggagccag
    11821 aaggatgata cgacctggac gaaagcaaac aaactggcag ccgcatggct accaagggtt
    11881 cgggttcttc atccatggcc tgtggagcgg ttcaccgcca gacacccgag gcaggagccc
    11941 ggtgcgtaaa tcgcgcacgc cgggatctgt
//
