LOCUS       NZ_CP040538             5596 bp    DNA     circular CON 08-JAN-2024
DEFINITION  Klebsiella pneumoniae strain CR-HvKP1 plasmid pCR-HvKP1-p5, complete
            sequence.
ACCESSION   NZ_CP040538
VERSION     NZ_CP040538.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN09487516
            Assembly: GCF_005853785.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Dong,N. and Chen,S.
  TITLE     Tracking microevolution events among ST11 carbapenemase-producing
            hypervirulent Klebsiella pneumoniae outbreak strain
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Dong,N. and Chen,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUN-2018) Department of Applied Biology and Chemical
            Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon
            000000, Hong Kong
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: v0.4.4 v. v0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50x
            Sequencing Technology             :: Illumina NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/08/2024 05:11:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,876
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,594
            CDSs (with protein)               :: 5,594
            Genes (RNA)                       :: 106
            rRNAs                             :: 8, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 76 of 176
            Pseudo Genes (incomplete)         :: 111 of 176
            Pseudo Genes (internal stop)      :: 29 of 176
            Pseudo Genes (multiple problems)  :: 38 of 176
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP040538.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="CR-HvKP1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pCR-HvKP1-p5"
                     /geo_loc_name="China"
                     /collection_date="2016"
     gene            join(5188..5596,1..101)
                     /locus_tag="FFT47_RS30995"
     CDS             join(5188..5596,1..101)
                     /locus_tag="FFT47_RS30995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            108..320
                     /locus_tag="FFT47_RS29855"
                     /old_locus_tag="FFT47_29850"
     CDS             108..320
                     /locus_tag="FFT47_RS29855"
                     /old_locus_tag="FFT47_29850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            987..1211
                     /locus_tag="FFT47_RS29860"
                     /old_locus_tag="FFT47_29855"
     CDS             987..1211
                     /locus_tag="FFT47_RS29860"
                     /old_locus_tag="FFT47_29855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            1195..1461
                     /locus_tag="FFT47_RS29865"
                     /old_locus_tag="FFT47_29860"
     CDS             1195..1461
                     /locus_tag="FFT47_RS29865"
                     /old_locus_tag="FFT47_29860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1531..1881)
                     /locus_tag="FFT47_RS29870"
                     /old_locus_tag="FFT47_29865"
     CDS             complement(1531..1881)
                     /locus_tag="FFT47_RS29870"
                     /old_locus_tag="FFT47_29865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(1947..2492)
                     /locus_tag="FFT47_RS29875"
                     /old_locus_tag="FFT47_29870"
     CDS             complement(1947..2492)
                     /locus_tag="FFT47_RS29875"
                     /old_locus_tag="FFT47_29870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(2518..2844)
                     /locus_tag="FFT47_RS29880"
                     /old_locus_tag="FFT47_29875"
     CDS             complement(2518..2844)
                     /locus_tag="FFT47_RS29880"
                     /old_locus_tag="FFT47_29875"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            complement(3202..3360)
                     /locus_tag="FFT47_RS31235"
     CDS             complement(3202..3360)
                     /locus_tag="FFT47_RS31235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            4000..4176
                     /locus_tag="FFT47_RS30595"
     CDS             4000..4176
                     /locus_tag="FFT47_RS30595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            4166..4510
                     /locus_tag="FFT47_RS29890"
                     /old_locus_tag="FFT47_29885"
     CDS             4166..4510
                     /locus_tag="FFT47_RS29890"
                     /old_locus_tag="FFT47_29885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4719..>5153
                     /locus_tag="FFT47_RS31095"
                     /pseudo=""
     CDS             4719..>5153
                     /locus_tag="FFT47_RS31095"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          3835..3914
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(3252..3649)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          3..436
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="1.21e-65"
     Typing          4501..5594
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.726"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ttctggtgct gccgaagggg acggccgtag acaggacgca gagctggacg gtcgggaacg
       61 ggcggtcgaa gcagaacgcg ctgagactgg tgaaggagta agtgccgatg accgagctgg
      121 aaaaacagtt gctgagcgca ttagagcagc tacagcagga ctactcgaaa aggctggacg
      181 agtgggagag cgccttcggg gaatggcgga aaatgtcagg gcttatgcaa cgggagaacg
      241 cggcgctgag cgagcgcgtg acgcgcttga gtcagcaggt ggaacgcttg agcgggcaac
      301 tgcagcgttt gagccggtag tgcagcgtca tgagatggcc gtggccgcag aacgggcgca
      361 cgagcaacgg cagcacgaaa aggaactgac ggaagcgcgt tcccggcagc gaagctatga
      421 cggtccgtcg ctgggctgaa atgcaggaaa acagggttct catcgggcat ttacgctgaa
      481 accggatgtc attcacctgg ctgcatggct atgcagccag gtaaaattta tctgtcgcga
      541 acggggcggt ttatcgggtg gtttgttgcc ggttttacct gactgccacc ccgttcgcgg
      601 cgaacccgtc cggggcggtg cgggcaacgg gtgttatgtt taatcagcgt gaacggcaga
      661 tacaaaaaag ccccggaggg cttttaacta ctgacttatt tttttgaaga tttgctgggt
      721 ttcaatttgt cagtatttcc cggcgttttt ttattgtcac gctggcgttt atcaggttcc
      781 cctgttgaat cggcaattcg tttcggaccc ggcttaagac ccataatcgt tatcctttca
      841 atagttaaga ggaaagccct caagctagat atattgaccg cgttacggat tatcaagagg
      901 ggctaacaag attatttttt gttcttttag gttgattgtg gtactgttga tatacatgta
      961 tagcaaatgg tgttcgggag gtttatatgc ttgctatccg gttatctgat gagattgagt
     1021 cccgtctgga ctcgctggcg aagcaaaccg gcagaacaaa gacgttttat gcgcgggaag
     1081 caatactggc gcatctggaa gacctggagg attattatct ttcagcagaa actgctgcac
     1141 gcgttcgccg tggtgatgaa gcagtgcatt cgtctgaaga cgtgaggaag tcacttggtc
     1201 tggacgatta actattccga tcgggcgctc aaatcgttac gcaagatgga caaacagaac
     1261 gcacgacgga ttgtggattt tatgagttta cgcattgcag ttgctgccga tcctcgccag
     1321 tcagggaagc cgctcaaagg tgagctgggc gagttctggc gctatcgggt gggagattat
     1381 cgcgttctgt gtgagatccg agatgacgag cttgttatcc ttgccgccac gattggacat
     1441 cgccgcgaag tttacgactg aagcccgcta tgcgggcttt tttgatgccg acaacaggta
     1501 ttatggtaaa tactcgacta actacgtgtt ctactgtttg tcggttttag ttaaccagca
     1561 ggttgtctta acgataatgt agccgttatt tttaactaat aaggtcatat tgttaacgtc
     1621 aggcataata aaagacatta tggcctcaga gttatcatca gaagcagctc tctgcattac
     1681 tacttcgatt cccttttctt tgaatctata agtatccagt tgcctattaa acttagaaag
     1741 acctacaacc atacttagtt tgttatcttt aacagtaaga gatgcttcct gagtaccatt
     1801 acatcggtag taagcggtgt aaccgtcagc aaaagatcga ccggggaata taaatgctag
     1861 gaacgcaatt aaccaataca tagttgagaa tcttggtatt acacatttca ctatcctctc
     1921 tttcattttt cgttcaccat actttgttat ctgattttta atttttctat tttataaacc
     1981 aagtctctac caaaaaggcg aaaattatcc tcaaataata ataattgttt aagaacatcc
     2041 atgaaagcag ctgcttcacc ctcagttatg ggagctactc tatataacat ataacctgag
     2101 ttaagggttt cttttatact ttcttcaatg ctcattataa atattagtgg attatcattt
     2161 ttcttccccc atgggtttct aattaatcct tcgatgtact caggtccttt gcgtcctatt
     2221 gcctgcgcaa tattttttat ttcatctgcg tgagggcttt gttgtacagc tttgtttatg
     2281 cctgcttgag tgattttcag agactctaca ccgtgtatgt tgtatgagat aaaatgtaat
     2341 aaattaagat gcaatgcaat taatccattt ttgtttacat ctgagtattt ggataaaaaa
     2401 cgtaatgaag tgttaactct cccttgaagc ctaacaattg ggagtgctgc cttgtatatt
     2461 tccttctccg ttaaataccg tttaaaaaac ataattctcc gcctccgtat agcgtgccta
     2521 ttttatttcg tcgtaaaact tagctctgaa atattccttt agaaccatca tgtcattaat
     2581 aattgaatct atctcatcca ttttttcttg gtattctttt aatggtgaag ggagcggtaa
     2641 ctcaatattt gaaaagtgat tggcgagata gggatcaagg tttctcatcc attcaggctt
     2701 ttcgccaatg cgttcttcaa ctgaaagtga acctgataat ctctggttaa taagatcact
     2761 tagactcatt ttggttttat tcgaaagccg ttgtaaggat tgctttaatc ctatatctag
     2821 catgatattt gttctttctc tcattacccc cccttgctat tgcgtttttt gttgcgcaca
     2881 aagctaccat tgcgcattaa gaaacgcaat aaaaaaggag ggagggagtg gtttgtacat
     2941 gatgtgcagt gcgctgaggg catcagtggc gggtgttttt ccgtgggatg gatggagctc
     3001 cggggataac agaactgacg atcatttaaa ggatcttctt gagatccttt ttttctgcgc
     3061 gtaatctctt gcactgtaaa cgaaaaaacc gcctggggag gcggtttgat cgaaggttaa
     3121 gtcagttggg gaactgctta accgggtaac tgggtatacg agaccgcaga taccaaaact
     3181 gttctttcag tgtagccgca gttaggcacc cacttcaaga cctctcaata tctctctcgt
     3241 atatatccag ttaccaatgg ctgctgccag tggcgttttt gcgtgtccgt ccgggttgga
     3301 ctcaagatga tagttaccgg aaagggcgca gcagtcgggc tgaacggggg gttcgtgcat
     3361 acagcccagc ttggagcgaa cgacctaaac cgagccgaga taccagcagc gtgagctatg
     3421 agaaagcgcc acgcttcccg aaagggaaaa aggcggaaca ggtatccggt aaacggcagg
     3481 gtcggaacag gagagcgcac gagggagccg ccggagagaa atcaccggca tctttaagtc
     3541 ctgtcgggtt tcgtcctcct ctgacttgag cgtccatttc tgtgatgctc gtcaggggag
     3601 gcggagccta tggaaaaacg gcaacgccgc atccttttct ccggcccttt ctgatttgag
     3661 cgcctttgct ttatcccgtt atccgtgagt cggtcacgcc cgccgcagcc gaacggcagg
     3721 agcgcagcta ccgagtgagc gaggaggcgt catttcatct gtccgtgcat ggtattgcgg
     3781 acgctcctga gccacatatc acacctgcca catgaggcac tttcatgaat cgtgctctca
     3841 tgccaacata gtaagccagt atacacagcg taaacgctgt gactggttca gggctgcgcc
     3901 ccgaaacccg ctaacaccac tgacgcgctg gcgcttgtcc aacgccggag cgacccggaa
     3961 aacttttccg tgccgtccgg cgtttgcgaa ggagggataa tgcagaagcc gaagaagaag
     4021 ccgctcgtcg taaaaaaaag atcctggcat ccggcagtga tggcaaatct gatgcgtcag
     4081 gcacaggagg aagattaccg ccgggagcag atggcagagc ttgagcgttc agcccggtca
     4141 cattttgcgg atatcggagg agaggatggc ggttaagcgt acccgttttt taggtatccg
     4201 ggtgaccgat ggggagtacc agcagctgct ggagcgctgc aatggaaggc aactggcggt
     4261 gtggatgcgg gaaacctgtc tcgatacgcg cccggcgcgg tcgttgcggc tgccgtccat
     4321 cgatccggtt ttgctgcgcc agcttgccgg gatggggaac aacctcaacc agatcgcccg
     4381 gaaaattaac ggcggccagt ggtcaggcgc tgaccgggtt caggtggtgg ccgcgctgat
     4441 ggccattgat gccgaactcg agcggctgcg gcatgccgtg ctggaaaagg gggcagacga
     4501 tgatcgttaa gtttcatccg cgagggcgag gcggcggtgc cgggccggtg gattatctgc
     4561 tggggaaaga ccgccagcgc gacggggcca tcgtcctgca gggaaagccg gaggaggtcc
     4621 gggagcttat cgacgcctcg ccctacgcca aaaagtacac ctccggcgtc ctgtcttttg
     4681 ctgaacagga tttaccgccc ggccagcgtg aaaagctgat ggcgagcttc gagcgggttc
     4741 tgatgcccgg actcgataaa gaccagtaca gcgtgctgtg ggttgaacac cgggacaagg
     4801 ggcggctgga actgaatttt ctggtaccga acacggaact gctgaccggc aggcgtctcc
     4861 agccgtacta cgaccgggca gaccgtccgc gcatcgatgc gtggcagacc gtggtgaacg
     4921 ggcgactggg gctgcacgac ccgaacgcac cggagaaccg gcgcgcgctg gtgacgccat
     4981 cttcgttacc caaaacgaag caggaagccg cagaggctat tacgcggggc ttactggccc
     5041 tcgcctcgtc aggggagctt aaaacgcgtc aggacgtcac tgaggcgctg gaaagcgcag
     5101 gttttgaggt cgtgcgcacc acaaagagca gcatcagcat tgccgacccg gacggagggc
     5161 gaaacatccg acttaaggga gccatctatg aacagtcttt taacgctggc gaaggactta
     5221 gagcagaaat cgaaagcgca gcagcagagt accggcgaaa tgctgaaagc cgcattcagc
     5281 gagcacgaga agtctgtcag agcggaactg agcgaaagcg ggaagagaat cagcgccgcc
     5341 atccgcgccc acgagcaggg tatgagcgcg gccatgcagt cgaaccgcct gagcgtgatg
     5401 cgtatggtcg ggcggacatg gctgaccatc acgatggtgt caggactgct gttcgccagc
     5461 ctgagcgggg tgttgtggta tcaggggagc ttgatagcgt caaatctggc cgaaatcgac
     5521 cggcagaacg cagcgctgtc gaagctgaac gcgaagacct ggggcgtgac gtacctggaa
     5581 gacagcaacg gacgtt
//
