LOCUS       NZ_CP040537            11970 bp    DNA     circular CON 08-JAN-2024
DEFINITION  Klebsiella pneumoniae strain CR-HvKP1 plasmid pCR-HvKP1-p4, complete
            sequence.
ACCESSION   NZ_CP040537
VERSION     NZ_CP040537.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN09487516
            Assembly: GCF_005853785.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Dong,N. and Chen,S.
  TITLE     Tracking microevolution events among ST11 carbapenemase-producing
            hypervirulent Klebsiella pneumoniae outbreak strain
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   Dong,N. and Chen,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUN-2018) Department of Applied Biology and Chemical
            Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon
            000000, Hong Kong
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: v0.4.4 v. v0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50x
            Sequencing Technology             :: Illumina NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/08/2024 05:11:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,876
            CDSs (total)                      :: 5,770
            Genes (coding)                    :: 5,594
            CDSs (with protein)               :: 5,594
            Genes (RNA)                       :: 106
            rRNAs                             :: 8, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 76 of 176
            Pseudo Genes (incomplete)         :: 111 of 176
            Pseudo Genes (internal stop)      :: 29 of 176
            Pseudo Genes (multiple problems)  :: 38 of 176
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP040537.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="CR-HvKP1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pCR-HvKP1-p4"
                     /geo_loc_name="China"
                     /collection_date="2016"
     gene            102..1787
                     /locus_tag="FFT47_RS29775"
                     /old_locus_tag="FFT47_29770"
     CDS             102..1787
                     /locus_tag="FFT47_RS29775"
                     /old_locus_tag="FFT47_29770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            1797..2054
                     /locus_tag="FFT47_RS29780"
                     /old_locus_tag="FFT47_29775"
     CDS             1797..2054
                     /locus_tag="FFT47_RS29780"
                     /old_locus_tag="FFT47_29775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            2139..2288
                     /locus_tag="FFT47_RS29785"
                     /old_locus_tag="FFT47_29780"
     CDS             2139..2288
                     /locus_tag="FFT47_RS29785"
                     /old_locus_tag="FFT47_29780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            3772..5736
                     /locus_tag="FFT47_RS29795"
                     /old_locus_tag="FFT47_29790"
     CDS             3772..5736
                     /locus_tag="FFT47_RS29795"
                     /old_locus_tag="FFT47_29790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            5736..6467
                     /gene="mobC"
                     /locus_tag="FFT47_RS29800"
                     /old_locus_tag="FFT47_29795"
     CDS             5736..6467
                     /gene="mobC"
                     /locus_tag="FFT47_RS29800"
                     /old_locus_tag="FFT47_29795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            6474..7004
                     /locus_tag="FFT47_RS29805"
                     /old_locus_tag="FFT47_29800"
     CDS             6474..7004
                     /locus_tag="FFT47_RS29805"
                     /old_locus_tag="FFT47_29800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(7031..7210)
                     /locus_tag="FFT47_RS29810"
                     /old_locus_tag="FFT47_29805"
     CDS             complement(7031..7210)
                     /locus_tag="FFT47_RS29810"
                     /old_locus_tag="FFT47_29805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(7236..7664)
                     /locus_tag="FFT47_RS29815"
                     /old_locus_tag="FFT47_29810"
     CDS             complement(7236..7664)
                     /locus_tag="FFT47_RS29815"
                     /old_locus_tag="FFT47_29810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(7828..8142)
                     /locus_tag="FFT47_RS29820"
                     /old_locus_tag="FFT47_29815"
     CDS             complement(7828..8142)
                     /locus_tag="FFT47_RS29820"
                     /old_locus_tag="FFT47_29815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(8130..8408)
                     /locus_tag="FFT47_RS29825"
                     /old_locus_tag="FFT47_29820"
     CDS             complement(8130..8408)
                     /locus_tag="FFT47_RS29825"
                     /old_locus_tag="FFT47_29820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(8583..8948)
                     /locus_tag="FFT47_RS30990"
                     /old_locus_tag="FFT47_29825"
     CDS             complement(8583..8948)
                     /locus_tag="FFT47_RS30990"
                     /old_locus_tag="FFT47_29825"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(8945..9316)
                     /gene="tolA"
                     /locus_tag="FFT47_RS29835"
                     /old_locus_tag="FFT47_29830"
     CDS             complement(8945..9316)
                     /gene="tolA"
                     /locus_tag="FFT47_RS29835"
                     /old_locus_tag="FFT47_29830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(9590..9835)
                     /locus_tag="FFT47_RS29840"
                     /old_locus_tag="FFT47_29835"
     CDS             complement(9590..9835)
                     /locus_tag="FFT47_RS29840"
                     /old_locus_tag="FFT47_29835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            10480..11967
                     /gene="ltrA"
                     /locus_tag="FFT47_RS29850"
                     /old_locus_tag="FFT47_29845"
     CDS             10480..11967
                     /gene="ltrA"
                     /locus_tag="FFT47_RS29850"
                     /old_locus_tag="FFT47_29845"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     BGC             1..6787
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(2392..2970)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5737..6464
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 gcgcacgccg ggatctgtgc ggggggtatc cggtaacggg tatccctacc gcgacatgtt
       61 ggctttaatt tattgtttta aatgtcaaag aggaaaacgg tatgagtggt ggagacggtc
      121 gaggtccggg taattcaggt ctgggacata atggtggtca ggccagtggg aatgtgaacg
      181 gtacgtctgg taaaggtggc ccttcatcag gtggtggtac ggatccaaac agcgggccgg
      241 gctggggtac gacgcatacg ccgaatggag atattcataa ctataatccg ggggagtttg
      301 gtaacggcgg gagtaaaccc ggtggtaatg gtggtaacag cggcaatcat agcggtagct
      361 ctggtggcgg acagtcttcg gccactgcga tggccttcgg tctgcctgcc ctggctactc
      421 cgggggctga aggactggct ttatcagttt ccggcgatgc gttgtcggcc gccgttgctg
      481 atgtgctggc tgccctgaag gggccgttta agtttggtct gtgggggatt gcgatctacg
      541 gtgtgctgcc ttctgagatt gcaaaagatg atccgaaaat gatgtcaaaa attatgacgt
      601 cattaccggc cgatgcggtg acggagactc cggcaagtac tttaccactg gaccaggcga
      661 cggttcgtgt cagacaacgg gttgtggatg tggtgaagga tgagcggcag catattgcgg
      721 ttgtcgcagg tcggccaatg agtgtccctg tggtggatgc gaaaccgaca aaacgtccgg
      781 gggtattcag tgtgtcgatt ccgggtctcc cgtctctgca ggtgagcgta ccgaaaggtg
      841 ttccgacagc gaaagccccg ccaaaaggca ttgttgctga aaaaggtgat tcacgtccgg
      901 ctggttttac agccggtggt aactcccgtg aggccgttat tcgtttcccg aaagagaccg
      961 gacagaagcc ggtttatgtg tcggtgacag atgttcttac cccggcacag gtaaaacagc
     1021 gtcaggagga agaaaagcgt cgccagcagg catgggacgc cgctcatccg gaagaggggc
     1081 tgaaaagaga ctatgataaa gcgaaagctg agctggatgc cgaagataaa aatattgcga
     1141 ccttaaacag ccgcattgca tcgacagaga aggcgctccc cggtgcaagg gctgctgttc
     1201 aggaagccga taaaaaggta aaagaggcag aggcaaataa agatgatttt gtgacttata
     1261 acccgcctca tgaatatggc tccgggtggc aggatcaggt tcgctatctt gataaggata
     1321 ttcagaatca gaatgagaaa ttaaaagcgg ctcaggcatc tttaaacgca atgaatgaat
     1381 ccttatccag agataaggct gcacttaccg gggcgatgga gagccggaaa caaaaggaga
     1441 aaaaagcgaa ggatgcagaa aataagttaa atgaggaaaa gaacaagcct cgcaagggga
     1501 ctaaagacta cggccatgat tatcatccag tacctaaaac agaagacata aagggacttg
     1561 gagagttaaa gcgaggtgat cctaaaacac ctaaacaagg tggtggtggt aagcgggccc
     1621 gatggtatgg cgataaaaaa cgtaaaattt atgaatggga ttcccagcac ggtgagcttg
     1681 aagggtatcg tgccagtgat ggcgaacacc taggcgcatt tgatcccaaa acgggtaagc
     1741 aggttaaagg gccagatcca aaacggaaca ttaaaaaata tctttgagag gtaaatatgg
     1801 ggcttaaatt acgtttgaca tggtttgata agaaaactga agagtttaaa ggggaagagt
     1861 attctaaaga ctttggtgac gatggttctg tcattgaaag cttgggaatg cctttaaagg
     1921 ataatattaa taatggtgag tttgatttga agaaagaatg ggttccttta ctacagccat
     1981 tttttaaaaa tcaaataaaa cctgatgtgg ataattgtta tatagctttt gattatcggg
     2041 acggtaactg gtaacaaaaa attatcaagg atgatttgaa atactgactt ccggtgttca
     2101 gggatgaatg ccggagtctt taaatacagg gaaagtatat gaaaaaggta aagactattt
     2161 ttctttttat attgattgtt gctggctttc ttctggccgc atgtcaggca aattatatcc
     2221 gggatgttca gggcggaacg gtggcaccat cgtcctcctc tgaactgacg gggatcgcgg
     2281 ttcagtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatcttttg
     2341 ccctgtaaac gaaaaaacca cctggggagg tggtttgatc gaaggttaag tcagttgggg
     2401 aactgcttaa ccgtggtaac tggctttcgc agagcacagc aaccaaatct gtccttccag
     2461 tgtagccgga ctttggcgca cacttcaaga gcaaccgcgt gtttagctaa acaaatcctc
     2521 tgcgaactcc cagttaccaa tggctgctgc cagtggcgtt ttgcgtgctt tcccgggttg
     2581 gactcaagtg aacagttacc ggataaggcg cagcagtcgg gctgaacggg gagttcttgc
     2641 ttacagccca gcttggagcg aacgacctac accgagccga gataccagtg tgtgagctat
     2701 gagaaagcgc cacacttccc gcaagggaga acggcggaca ggtatccggt aaacggcagg
     2761 gtcggaacag gagagcgcaa gagggagcga cctaccggaa acggtgggga tctttaagtc
     2821 ctgtcgggtt tcgcccgtac tgtcagattc atggttgagc ctcacggctc ccacagatgc
     2881 accgaaaaag cgtctgttta tgtgaactct ggcaggaggg cggagcctat ggaaaaacgc
     2941 caccggcgcg gccctgccgt tttgcctcac atgttagtcc cctgcttatc cacggaatct
     3001 gtgggtaact ttgtatgtgt ccgcagcgcc cgccgcagtc tcacgcccgg agcgtagcga
     3061 ccgagtgagc gaggaggcgc aatttccccg gcacctgcaa gacggtcacc ggacgcgctg
     3121 atgtgggata ttgtcccctg attaagtctt tctattccct gacgatcaat tatcaggata
     3181 ggtgaaccat gtcggttttt cgctgaacat tttacgttaa cctatctctg ttgctttttt
     3241 ctccttgcgg agaaatgcaa gtcatccccg ctggggcgtg gctgacttca ggggaaaaga
     3301 ccttgcttac gctggctgga cgccaccgac gcacggtctt ttcccctctt tctggctctg
     3361 tccggctgga cgccggccac gccagaaatt cacccggccc ccgtcaggct cacgcctgac
     3421 cacccacaaa aatccacggt gctggacgca cctgacgaag cagcaaagct gctcctgatt
     3481 ttgcgggtgg tcagccgctc gctatggcga actctgacgg gggccggaac accggaaaag
     3541 accgcactgg ggtgccgcct tttcctctca cacgctgggg cgtgctcgtt gttcctccgg
     3601 ggctgcgcct tttccgcatg gggcggaaag aggctggcct ccgcggtgaa agctgaacgc
     3661 aatcaccacg gacgtgaccg ccgccgccat gccttggggc atgtccgcgg cttgtgcagc
     3721 gggggctgcg catcacgccg gtaaacccgt cgtctgaagg agagagattg catgtttaat
     3781 acggattcgc ttgcctggca gtggcatatc ccggagctgg cccggcagat ttacccgtgg
     3841 gtcagctggc tgtaccggga gtattacagt ctggccggtt tttttctggt tatcggcctg
     3901 tttttcggtc tggctgaact ctgggccggc cgaatgcgtt ttgtggggct gagccataag
     3961 gcgcggcggg agtatgccga tcgctataag cgcgataata accggactgt gccattttcg
     4021 gtgctggcct cgctggattc acccgggcgg atggtcaggg cacggcgaca tgtctggaac
     4081 tacgcgtact ggctgaccgg cgctgccggt ctggcccttc tgggggggct ggttgtcgcc
     4141 gtgtttatcg agagctgggg agaaaccgtc ctgcgcccgg acctctatgc gaacgcagat
     4201 tattacctgc tgcagccggc taacctgttc gggatagcct ggtgtgccgg agggcttgtc
     4261 gccggcgtcc ttctggcgcg gctgctggcg ttcctgctgc ttgatggtca ttttgcggcc
     4321 gctgacgagg cggtgaacgc gaaactgaat caggagagcc ggcgcagcag ccagcggacc
     4381 ggagaaatga cagacgtccg ccacctgcat ttcggggagc cggtaccggt taacgcgctg
     4441 gccgattttt ccacggagca ggcccggaaa cagcaggcgg tgtttctggg taaggatgag
     4501 cagggccagc cggtgctggt ccctcgtgat acctggcgca agaccaatat tcagatcctc
     4561 ggcctgcccg ggagcggcaa gagcgcgatg gccaccaacg cgctcattcg ctgcgtacgc
     4621 gatttcgggg acgctgtggt gtacttcgac cccaaagggg acgcgtgggc gccgcacgtc
     4681 ttccgggcgc actgcccgga cttcaccctg ctggatctgc ggccggggaa accggcgcag
     4741 ctgaatctgt tccgcgatct cgaccagtac gccctgaaga atctgctggt tgccgggttt
     4801 aacctgagtg agaccggcga cgtggcggac cactaccgca tcagcgagca gaaagcggcg
     4861 aagctgattg cggagcagtt cccgcagggg gcaaacattc agcaggtgct ggcggcggcg
     4921 tacgcgttgc cggaggccct gaaaaaggac gtgaaggggc tgatcaccaa gctggagaac
     4981 gtggccgacc tgagcgtcct gcagacggac agcggtatcg atgtggcggg gataatcaac
     5041 ggcggcggct gcctgtacgt catcggctcg atggacgatg aggcggtgat ccgggtgcag
     5101 aagatgctgt ttgcccgctg cgcgcaaatc atcattgcgc gggatgagtt ccggaggtgg
     5161 ccgcacgcga gcatcatgct cgatgagatt aaatatctgc tgtcgaagta cgtgctgaac
     5221 gcgctgggga cgctgcgcag ccgggactgc aacctgctgc tcgcgcacca gtcgctgggc
     5281 gacttcggtc agtgcgggca ggatctgccc gctgattttg tgaaaaccac ggtactggat
     5341 aacacgccga tccgctggtt ctatcgcgcg gccagccagg agtccgcgca gtgggcggcg
     5401 ggccagaccg gggagatccg ggtggacgtg gagcgccgcc gggccagccg ggaggcgggg
     5461 aacgtggagc acatcagcgg cgacagcttc atccagaagg aggcgcggcc gctgtttgac
     5521 gtgaacaccc tgcaacacct gcctgacggg ttcgcggtga tgaccgggct gggcgtggcg
     5581 cggctggcgt tcagcagccc gctgcgggtt gagcgccggg aaatttcgct gaaaaccttc
     5641 ccggtgctgg cgaaaacgga cccgctggcg gagtacctgc ctccggccga cggcggcgga
     5701 ccaaaagcgg atgatgattt tgcggggctt tactgatgac actggagcag tacaacgtca
     5761 gccacgcgaa gcggcaggcg cggaacgcgg agaaaacacg gctgaccctg cgctggctgc
     5821 gggaagagct gtgttccacg gcggagctgg ttgcgcgacg gctggggatt gcggcggtac
     5881 agccggttta ccgcttcctg gacagtctgg tggcgaaagg gctgctggtc cgggcgaaat
     5941 acccggttga cggccgtcag gtcagcgtgt gggggctgac gccgcacggg gtggcgttct
     6001 cgttcgacga ggacgagccg ctgacggacg ttattccgtt tcagccgtca agggtgtcgg
     6061 cggcgcagct gccgcaccgg ctggccgtgc agtccctgcg cctctccatg gagggccggg
     6121 gggccaccgg ctggcggtac ctgcaccgta tggccctgaa agggatgaaa gtgccggacg
     6181 cgctggcgga gctggacggc agaacggtgg catttgaggt agagcgcacc gtcaagagcc
     6241 gcaggcggta tcaggaagtg gtgtccgggt atctgttcaa ccgccgggcg aacggcatcg
     6301 atgaaatctg gtacatctgc cctgaccggc cgacgcttgt ccgggtacag cgggcgattc
     6361 tggcggtgga tgaaatcgtg aatccacaga ccggcgaggc ccgcaaaacg gtggagctgg
     6421 accgggagag gctgttcgcg tgctttaaat ttatgacaac ggagtgaggg attatggcgc
     6481 tgtacatggt atttattgag gacgtggaag acaaagacgg cgagcgctgc attatgccaa
     6541 ccagcgagca tctggaagac atggtggtgc tcgcctggca gaccggggcg tatgttttct
     6601 gttttgtcga ccgggcccag catctggcga tgatgaaatc catcgggatg tatgacgagc
     6661 ggctcaacag cgacgaaaaa gccacgtact ttttcaccgg tgaaaatccg caatgggaac
     6721 gggacaaacc cacaggaatg aagctgtgtg attacctcta tcgaaaggtg gagcggttcg
     6781 ccatcgagaa cggtgtgcag gtggttgatg ccgggaagtg ctcaccggag cagattaacg
     6841 cgatgattga ggtggtcagc gagcggcacg gggaaagcgc ggtgaacgag aagctgaaag
     6901 cggagctgat gcgggaatat aacagccgct attttaacgt gccggtgcct gaactgccgg
     6961 aggacaaacc cgcccgccgg tggtggccgt tcggcaaaaa ataacgccag caccggcatg
     7021 gccggtgctg ttattcttcc ccgagcttat cccgcatcac ctgccagagt gtttccgcat
     7081 cctcgtgaag ccgttcgcac atgtcggccg gggtatccat gtccagctcg ttcagctttt
     7141 ccagcaacag cagcgactga tccttaatga atttagccat attgagggca gttttttcct
     7201 gcttcgtcat gttctccatt cccttcggta agttctcagc taaaccagcc ggaccagaag
     7261 cgttttttct tctgtgtcag ttctgttgtc aatctgtcca cttcggcctg gagtttccgg
     7321 cgctcatcat cgtgctggcg gcgttcttct tcccgggatg ttttatcttc cagcatcagg
     7381 gtgacagcct gccggagatc ggccagttcc ttttgcattg ccagaagctg ttcctgtggg
     7441 aaattttttt gtgtattttc acggtgtgaa atgccgggaa tttgtttctc agggatattc
     7501 acatttccgt atacacggat cagttctgac acatccacga cagggttgtt tttcccgtca
     7561 cgtgacacgg taacctttcc ctgttttaca tgattgtata gcgttcttct ggtaatgccc
     7621 gcggcttgtg ctgcctgtga aaggttgagc aatgttttcg ccactgtata cccctgattt
     7681 tatcgggtgt gaaaggtgtg taatttctca cggtaaatgt atcgctgaat tttacccgtt
     7741 tttccgttca ggatgtggtt ttctgacgcg ctgcgcttgt caaaccggcg acggccacgc
     7801 aatgaattgc gcccccgtca tcggttatta gaggctgtct gcccccggat acccggattc
     7861 tttctgatgc ctgacggtga cgatcacaat ctggtccatt tcccggtcgt ggcggtagag
     7921 catcacatag ccactgtcgc caaacccgat caccagttcc ttatattcca gcggcagaaa
     7981 cggcaccggg cggccaatgt ctggcaatgt tttcagttgc tggatagccc ggacgatcac
     8041 ctcacctcct tttctggctg ccagccgatt tttggttttg agaaagtcct gaaggcgttg
     8101 taaatcgcgc tgtgccagcg cggaaatcgt tacctgtggc atggcggagc atcctgctcg
     8161 ttctcagttc cccaggtact tatccaggct tccgcttctt ccgcagtaag gtgcagaccc
     8221 gtttcctgat agtgctgcca tgcggcctgt ccgtcccgca ggtactggct gcgcttctct
     8281 tcccggtcga tgtattcggt gattgcctcc agcattagcg cgtgcgctga gcggtgccgg
     8341 tcatcggcca gcgttttcag ccggtcctta agttcttcat cgagcctgat ggatgttgct
     8401 gatgacataa aagccccttt gtagtcatat gtattacaca tgactacttt aatccgtggc
     8461 ggcgctattt gccagaaata cggcttttgc tgcccatttt tcagcttcgc ttccactaat
     8521 cagggctgct acaggctcac tcatcatcca ggactattgc agcctgcggg ttaaaggacc
     8581 tgttactggc ggcaaccatc atcattgagc ttttgggtgg tgactggtcg cggatcctgt
     8641 aagccgtcat ttttcagact gaattcaaaa tttataacct gtcgcattgc cgggtgccct
     8701 gcgggatata gccagtgcct cattgccgtc agcgtttccc gaaaaaaaag gtgacagccg
     8761 gtaagcgaca cgttgtccac tcgtccatcg tccagaatat caaacgtggc ggtcacctga
     8821 ccttcgatgc gttttgctgc ggcctgtacc gggtaggttg gctgcagtgt ggttacgggt
     8881 tttagtggta tcgcatgtac cggagtgagg aacatggccg ccagcactgt cagagtaaaa
     8941 gctttcatgg tctgaaatcc agaggggcgt ttttgaatat ttgccaggta ttttcatccg
     9001 gtgccggcgg aattttggcg tgggtaacgg ctgatattgc tgctttacag agtttcggat
     9061 ctcctccttc agcaatggcg ctgttcagag tgccatcacg ttgtatggat atccgcacgg
     9121 tgcacatttt tcccgcatag gcctctggtt caaacatctg catttcaaca gcggtcttga
     9181 tggccgcagc gtagctactg attttagatg acttatccag agtataaatt ttcggcggct
     9241 gggtggtgga atgcttacag gccgtcagga gtaaggggct gcacaatgcc agcaggataa
     9301 agggtattaa acgcatcggg ttgatgacct gtcagtaaaa aatgttacat gtatatttgt
     9361 cactctttac ggccacgttc atttacataa acacggctta tcgtatcgtt tttacaccaa
     9421 acggcccgcc atgcagttgc gtgcataggc atgcagcggg ttacaattta ccgggcgcgt
     9481 ttccggcggt tttccgggtg gtttgttgcc tgttttatcc cgtagccgcc ggaaacgccc
     9541 tcagaccgtc tgagcggtgc gggcaatgcg acgttatggt aaatacccct taccggcggc
     9601 gctgtggctg ccaggtgaag gtggtttccg tagcctcgta tttctcccga cgcatttcct
     9661 gcgcccgcct ggcttcacgt tcgtcctgaa tggcacgaag caccctttcc cgcacctgtt
     9721 gttttaccgc ctttggcagc gttttgttcc ctttcaggcc catggccagt gcctgagcga
     9781 caaaggcgtc cgccaggcgc ttttcttcag cgggagtcag tttcaggtgc gtcatagtgg
     9841 cctccgggcg tctcaggttt cccgaacagt ttaaccgcag gtcctctcct catgccagtt
     9901 agctttcaga tgtgtctata ccatctgaaa tgtaagtgtg atgaatgtct taaataataa
     9961 aatttgttgc catgagaaat tttcgggaaa agacgcgatc tgcttacagg tggctccggg
    10021 cgcttttttg tgcctgaaga tgagaactaa tctcatatat tgacacctga aaacgggagg
    10081 agtaaggtaa taattatact gtgtatatgt acagtgtgcg ccgggagacc ggttagagat
    10141 caaggggtga aagtccccga ccattgaagg accagcaatc cacaaggtcc ccgagtcatg
    10201 cgttgcatac cgcgaggtat ggggcgaagc gttgacaggg gtgttgacag gccagccatt
    10261 gagccacgaa atgtatatta aattaccggg tgccgacgtt gtactgttaa cggaaggcaa
    10321 catcataggg tgcgatactg cgagtgccac atggacccgg cggggtctga gaccctggca
    10381 tgtcaatacg atctctacgc gggaaccggg agatctcccc tctgaccatc tgccagtgtc
    10441 ggagatggcc cgcaccggga agacgaggag tcatagccgg tgatgtacgg agaggagaag
    10501 tcggactcgc tcatagtagc ggcgaagcag gcgaacaacc cgaaaggagc ggagtcagtg
    10561 gagcgaagga gcggggccaa ggggaacgcg gaacagccac acatgcgccg gacacagagc
    10621 cgggaaagca tgtcacagag gctgtcacgc gtgcgggaag ctgcgaagca gcggaagaaa
    10681 gaacggttta cagcattgtt ccacctgctg acagtcgaag cactggaagc cgcattcctc
    10741 tccctgagca ggaaagcggc cgccggagtg gatggcatca ggtggatgga ctacgccgga
    10801 aacatgaaga acaacataac agatctgcac cggaggctac atcagggcag ctacagggcg
    10861 cagcccggca ggcgtcacta catcccaaaa gcggatggaa aacaacgccc gctcggcatc
    10921 gcctcgctgg aggacaagat cgtccagtat gcgctggtga aaatcctgaa cgcagtctat
    10981 gaaaacgact ttatggggtt ctcatacggg ttcagacccg ggcgaagcca gcacgatgca
    11041 ctggacgcac tggccacagg gctggtacgc actaacgtaa actgggtact ggatgccgac
    11101 atcagtcagt tcttcgacag ggtgagccac gaatggctga tcaggttcac agagcatcgg
    11161 atcggcgacc ggagggtaat caggctcata cgtaagtggc tcacagccgg gacgtcggag
    11221 gagggtcaat ggcgagcaac ggaggaaggc accccacagg gtgcggtcat ctcaccgctg
    11281 ctggcaaaca tatacctcca ctacgtcttc gatctgtggg cgcatcagtg gcgacgtcgc
    11341 tatgccacag gcaatgtggt aatggtcaga tacgccgatg acatcgtcat cgggttcgac
    11401 aaacgatacg atgcccggcg cttccgtata gccatgcagc gcagactgag ggagttcgga
    11461 ctcacggttc acccggagaa aacccgtctg atggagttcg gccgcttcgc tgccgaaaac
    11521 cgtgccatca ggggaaaagg caaaccagaa acgttcaact tcctcgggtt cacgcacatc
    11581 agcgggaaag atcgcaacgg caggttcatg ctgatacgaa agacccgccg ggatcggatg
    11641 acggcaactc tgaaagccat caaagacggt ctgcgaaggc gctggcatta ctcaatcccc
    11701 gaacagggaa aatggctcag gagagtggtt cagggatacc tgaactatca ctcggtaccg
    11761 ggcaacttcc ccaccatgca gaagttcagg acacacgtaa caaacctctg gcgccgggcg
    11821 ctcaggcgca ggagccagaa ggatgatacg acctggacga aagcaaacaa actggcagcc
    11881 gcatggctac caagggttcg ggttcttcat ccatggcctg tggagcggtt caccgccaga
    11941 cacccgaggc aggagcccgg tgcgtaaatc
//
